BMRB Entry 4721
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4721
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Title: Structure of the Central Core Domain of TFIIEbeta with a Novel Double-stranded DNA-binding Surface
Deposition date: 2000-04-14 Original release date: 2000-12-18
Authors: Okuda, M.; Watanabe, Y.; Okamura, H.; Hanaoka, F.; Ohkuma, Y.; Nishimura, Y.
Citation: Okuda, M.; Watanabe, Y.; Okamura, H.; Hanaoka, F.; Ohkuma, Y.; Nishimura, Y.. "Structure of the Central Core Domain of TFIIEbeta with a Novel Double-stranded DNA-binding Surface" EMBO J. 19, 1346-1356 (2000).
Assembly members:
general transcription factor TFIIE-BETA, polymer, 81 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
general transcription factor TFIIE-BETA: ALSGSSGYKFGVLAKIVNYM
KTRHQRGDTHPLTLDEILDE
TQHLDIGLKQKQWLMTEALV
NNPKIEVIDGKYAFKPKYNV
R
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 601 |
| 13C chemical shifts | 256 |
| 15N chemical shifts | 87 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TFIIE-BETA | 1 |
Entities:
Entity 1, TFIIE-BETA 81 residues - Formula weight is not available
| 1 | ALA | LEU | SER | GLY | SER | SER | GLY | TYR | LYS | PHE | ||||
| 2 | GLY | VAL | LEU | ALA | LYS | ILE | VAL | ASN | TYR | MET | ||||
| 3 | LYS | THR | ARG | HIS | GLN | ARG | GLY | ASP | THR | HIS | ||||
| 4 | PRO | LEU | THR | LEU | ASP | GLU | ILE | LEU | ASP | GLU | ||||
| 5 | THR | GLN | HIS | LEU | ASP | ILE | GLY | LEU | LYS | GLN | ||||
| 6 | LYS | GLN | TRP | LEU | MET | THR | GLU | ALA | LEU | VAL | ||||
| 7 | ASN | ASN | PRO | LYS | ILE | GLU | VAL | ILE | ASP | GLY | ||||
| 8 | LYS | TYR | ALA | PHE | LYS | PRO | LYS | TYR | ASN | VAL | ||||
| 9 | ARG |
Samples:
sample_1: general transcription factor TFIIE-BETA, [U-15N; U-13C], 1 – 2 mM; phosphate buffer 20 mM; NaCl 500 mM
sample_3: general transcription factor TFIIE-BETA1 – 2 mM; phosphate buffer 20 mM; NaCl 500 mM
sample_cond_1: pH: 6.0; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | not available | not available | not available |
| 3D_13C-SEPARATED_NOESY | not available | not available | not available |
| 3D_15N-SEPARATED_NOESY | not available | not available | not available |
| 4D_13C-SEPARATED_NOESY | not available | not available | not available |
| HNHA | not available | not available | not available |
| HMQC-J | not available | not available | not available |
Software:
NMRPIPE v1.6 - PROCESSING
PIPP, CAPP, STAPP v3.9 - DATA ANALYSIS
EMBOSS v5 - STRUCTURE CALCULATION
NMR spectrometers:
- Bruker AMX 500 MHz
Related Database Links:
| PDB | |
| DBJ | BAE91442 BAG73195 |
| EMBL | CAA45069 |
| GB | AAB20414 AAG39077 AAH30572 AAV38582 AAX36090 |
| PRF | 1802389A |
| REF | NP_001247856 NP_002086 XP_001168104 XP_002756966 XP_003269588 |
| SP | P29084 |
Download simulated HSQC data in one of the following formats:
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