BMRB Entry 4811
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4811
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Title: 1H and 15N Chemical Shift Assignments for Apo-Recombinant Bovine alpha-Lactalbumin PubMed: 11273708
Deposition date: 2000-08-17 Original release date: 2000-11-13
Authors: Wijesinha-Bettoni, Ramani; Dobson, Christopher; Redfield, Christina
Citation: Wijesinha-Bettoni, Ramani; Dobson, Christopher; Redfield, Christina. "Comparison of the Structural and Dynamical Properties of Holo and Apo bovine Alpha-lactalbumin by NMR Spectroscopy" J. Mol. Biol. 307, 885-898 (2001).
Assembly members:
apo bovine alpha-lactalbumin, polymer, 124 residues, Formula weight is not available
Natural source: Common Name: bovine Taxonomy ID: 9909 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bos Primigenius
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
apo bovine alpha-lactalbumin: MEQLTKCEVFRELKDLKGYG
GVSLPEWVCTTFHTSGYDTE
AIVQNNDSTEYGLFQINNKI
WCKNDQDPHSSNICNISCDK
FKDDDLTDDIVCVKKILDKV
GINYWLAHKALCSEKLDQWL
CEKL
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 115 |
| 1H chemical shifts | 217 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | apo-BLA | 1 |
Entities:
Entity 1, apo-BLA 124 residues - Formula weight is not available
| 1 | MET | GLU | GLN | LEU | THR | LYS | CYS | GLU | VAL | PHE | ||||
| 2 | ARG | GLU | LEU | LYS | ASP | LEU | LYS | GLY | TYR | GLY | ||||
| 3 | GLY | VAL | SER | LEU | PRO | GLU | TRP | VAL | CYS | THR | ||||
| 4 | THR | PHE | HIS | THR | SER | GLY | TYR | ASP | THR | GLU | ||||
| 5 | ALA | ILE | VAL | GLN | ASN | ASN | ASP | SER | THR | GLU | ||||
| 6 | TYR | GLY | LEU | PHE | GLN | ILE | ASN | ASN | LYS | ILE | ||||
| 7 | TRP | CYS | LYS | ASN | ASP | GLN | ASP | PRO | HIS | SER | ||||
| 8 | SER | ASN | ILE | CYS | ASN | ILE | SER | CYS | ASP | LYS | ||||
| 9 | PHE | LYS | ASP | ASP | ASP | LEU | THR | ASP | ASP | ILE | ||||
| 10 | VAL | CYS | VAL | LYS | LYS | ILE | LEU | ASP | LYS | VAL | ||||
| 11 | GLY | ILE | ASN | TYR | TRP | LEU | ALA | HIS | LYS | ALA | ||||
| 12 | LEU | CYS | SER | GLU | LYS | LEU | ASP | GLN | TRP | LEU | ||||
| 13 | CYS | GLU | LYS | LEU |
Samples:
apo-bla_15N_sample: apo bovine alpha-lactalbumin, [U-15N], 2.0 mM; EDTA 15 mM; NaCl 0.5 M
Ca2+_free_conditions: ionic strength: 0.5 M; pH: 7.1; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | apo-bla_15N_sample | not available | Ca2+_free_conditions |
| 3D 1H-15N NOESY-HSQC | apo-bla_15N_sample | not available | Ca2+_free_conditions |
| 2D 1H pre-TOCSY COSY | apo-bla_15N_sample | not available | Ca2+_free_conditions |
Software:
FELIX v2.30 - data processing
NMR spectrometers:
- Home-built . 750 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts