BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4961

Title: 1H, 15N and 13C assignments of the N-terminal domain of Yersinia outer protein H in its apo form and in complex with a phosphotyrosine peptide representing the putative binding site on host protein target p130Cas

Deposition date: 2001-02-22 Original release date: 2001-09-27

Authors: Khandelwal, Purnima; Keliikuli, Kai; Smith, Craig; Saper, Mark; Zuiderweg, Erik

Citation: Khandelwal, Purnima; Keliikuli, Kai; Smith, Craig; Saper, Mark; Zuiderweg, Erik. "Letter to the Editor: 1H, 15N and 13C assignments of the N-terminal domain of Yersinia outer protein H in its apo form and in complex with a phosphotyrosine peptide"  J. Biomol. NMR 21, 69-70 (2001).

Assembly members:
Yersinia outer protein H, polymer, 136 residues, 15000 Da.
DEpYDDPF, polymer, 7 residues, Formula weight is not available

Natural source:   Common Name: Yersinia pestis   Taxonomy ID: 632   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Yersinia pestis

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Yersinia outer protein H: MNLSLSDLHRQVSRLVQQES GDCTGKLRGNVAANKETTFQ GLTIASGARESEKVFAQTVL SHVANVVLTQEDTAKLLQST VKHNLNNYDLRSVGNGNSVL VSLRSDQMTLQDAKVLLEAA LRQESGARGSHHHHHH
DEpYDDPF: DEXDDPF

Data sets:
Data typeCount
13C chemical shifts479
15N chemical shifts123
1H chemical shifts647

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1YopH subunit 11
2phosphotyrosine peptide2

Entities:

Entity 1, YopH subunit 1 136 residues - 15000 Da.

1   METASNLEUSERLEUSERASPLEUHISARG
2   GLNVALSERARGLEUVALGLNGLNGLUSER
3   GLYASPCYSTHRGLYLYSLEUARGGLYASN
4   VALALAALAASNLYSGLUTHRTHRPHEGLN
5   GLYLEUTHRILEALASERGLYALAARGGLU
6   SERGLULYSVALPHEALAGLNTHRVALLEU
7   SERHISVALALAASNVALVALLEUTHRGLN
8   GLUASPTHRALALYSLEULEUGLNSERTHR
9   VALLYSHISASNLEUASNASNTYRASPLEU
10   ARGSERVALGLYASNGLYASNSERVALLEU
11   VALSERLEUARGSERASPGLNMETTHRLEU
12   GLNASPALALYSVALLEULEUGLUALAALA
13   LEUARGGLNGLUSERGLYALAARGGLYSER
14   HISHISHISHISHISHIS

Entity 2, phosphotyrosine peptide 7 residues - Formula weight is not available

1   ASPGLUPTRASPASPPROPHE

Samples:

sample_1: Yersinia outer protein H, [U-13C; U-15N], 0.6 – 0.9 mM; DEpYDDPF0.6 – 0.9 mM

cond_set_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCAsample_1not availablecond_set_1
HN(CO)CAsample_1not availablecond_set_1
HNCOsample_1not availablecond_set_1
HN(CA)COsample_1not availablecond_set_1
HNCACBsample_1not availablecond_set_1
HN(CA)HAsample_1not availablecond_set_1
(H)CCH TOCSYsample_1not availablecond_set_1
HCCH TOCSYsample_1not availablecond_set_1
13C NOESYsample_1not availablecond_set_1
15N NOESYsample_1not availablecond_set_1

Software:

NMRPipe -

NMRView -

XEASY -

NMR spectrometers:

  • Bruker AMX 500 MHz
  • Varian Inova 800 MHz

Related Database Links:

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