BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5005

Title: Backbone Sequential Resonance Assignments of Yeast iso-2 Cytochrome c, Reduced and Oxidized forms   PubMed: 11885986

Deposition date: 2001-05-01 Original release date: 2002-06-26

Authors: Benavides-Garcia, Maria; Rivera, Edna; Ramos, William; Hinck, Andrew; Nall, Barry

Citation: Benavides-Garcia, Maria; Rivera, Edna; Ramos, William; Hinck, Andrew; Nall, Barry. "Letter to the Editor: Backbone Sequential Resonance Assignments of Yeast iso-2 Cytochrome c, Reduced and Oxidized forms"  J. Biomol. NMR 22, 93-94 (2002).

Assembly members:
yeast iso-2 cytochrome c, oxidized form, polymer, 112 residues, 12958 Da.
HEC, non-polymer, 618.503 Da.

Natural source:   Common Name: Baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota Fungi

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
yeast iso-2 cytochrome c, oxidized form: AKESTGFKPGSAKKGATLFK TRCQQCHTIEEGGPNKVGPN LHGIFGRHSGQVKGYSYTDA NINKNVKWDEDSMSEYLTNP AKYIPGTKMAFAGLKKEKDR NDLITYMTKAAK

Data sets:
  • assigned_chemical_shifts
Data typeCount
1H chemical shifts360
13C chemical shifts418
15N chemical shifts104

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1yeast iso-2 cyt c1
2oxidized heme2

Entities:

Entity 1, yeast iso-2 cyt c 112 residues - 12958 Da.

1   ALALYSGLUSERTHRGLYPHELYSPROGLY
2   SERALALYSLYSGLYALATHRLEUPHELYS
3   THRARGCYSGLNGLNCYSHISTHRILEGLU
4   GLUGLYGLYPROASNLYSVALGLYPROASN
5   LEUHISGLYILEPHEGLYARGHISSERGLY
6   GLNVALLYSGLYTYRSERTYRTHRASPALA
7   ASNILEASNLYSASNVALLYSTRPASPGLU
8   ASPSERMETSERGLUTYRLEUTHRASNPRO
9   ALALYSTYRILEPROGLYTHRLYSMETALA
10   PHEALAGLYLEULYSLYSGLULYSASPARG
11   ASNASPLEUILETHRTYRMETTHRLYSALA
12   ALALYS

Entity 2, oxidized heme - C34 H34 Fe N4 O4 - 618.503 Da.

1   HEC

Samples:

sample1: yeast iso-2 cytochrome c, oxidized form, [U-13C; U-15N], 2.0 mM

condition1: pH: 6.0; temperature: 293 K; ionic strength: 0.13 M

Experiments:

NameSampleSample stateSample conditions
HSQC COSYsample1not availablecondition1
HNCACBsample1not availablecondition1
C(CO)NHsample1not availablecondition1
HNCOsample1not availablecondition1
HN(CA)COsample1not availablecondition1
HBHA(CO)NHsample1not availablecondition1

Software:

NMRPipe v1.8 - processing

NMR spectrometers:

  • Bruker AMX 500 MHz

Related Database Links:

REF NP_010875
SWISS-PROT P00045
GenBank AAD13974 AAT93051 AAB59339 AAB65003 AAA34940
EMBL CAA24606
PDB
BMRB 5003

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts