BMRB Entry 5090
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5090
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Title: Letter to the Editor: 1H(N), 15N, 13CO, 13C[agr], 13C[bgr] Assignment and Secondary Structure of a 20 kDa [agr]-L-fucosidase from Pea using TROSY PubMed: 11991358
Deposition date: 2001-07-19 Original release date: 2002-08-22
Authors: Codina, Anna; Gairi, Margarida; Tarrago, Teresa; Viguera, Ana Rosa; Feliz, Miguel; Ludevid, Dolors; Giralt, Ernest
Citation: Codina, Anna; Gairi, Margarida; Tarrago, Teresa; Viguera, Ana Rosa; Feliz, Miguel; Ludevid, Dolors; Giralt, Ernest. "Letter to the Editor: 1H(N), 15N, 13CO, 13C[agr], 13C[bgr] Assignment and Secondary Structure of a 20 kDa [agr]-L-fucosidase from Pea using TROSY" J. Biomol. NMR 22, 295-296 (2002).
Assembly members:
pea alfa-L-fucosidase, polymer, 195 residues, Formula weight is not available
Natural source: Common Name: Pea Taxonomy ID: 3888 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Pisum sativum
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
pea alfa-L-fucosidase: GSHMEDVEQVLDINGNPIFP
GVQYFILPAIRGPAGGGVRL
GRTGDLTCPVTVLQDRQEVK
YGLPVKFVIPEISPGIIFTG
TPIEIEYAKKPNCAKSSKWL
VFVDNVIQKACVGIGGPENY
PGVQTLSGLFKIEKHESGFG
YKLGFCVKGSPTCLDVGRFD
NDEDGRRLNLTEHESFQVVF
IQAEANDAEFIKSVV
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 156 |
| 13C chemical shifts | 505 |
| 15N chemical shifts | 156 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | alfa-L-fucosidase | 1 |
Entities:
Entity 1, alfa-L-fucosidase 195 residues - Formula weight is not available
| 1 | GLY | SER | HIS | MET | GLU | ASP | VAL | GLU | GLN | VAL | ||||
| 2 | LEU | ASP | ILE | ASN | GLY | ASN | PRO | ILE | PHE | PRO | ||||
| 3 | GLY | VAL | GLN | TYR | PHE | ILE | LEU | PRO | ALA | ILE | ||||
| 4 | ARG | GLY | PRO | ALA | GLY | GLY | GLY | VAL | ARG | LEU | ||||
| 5 | GLY | ARG | THR | GLY | ASP | LEU | THR | CYS | PRO | VAL | ||||
| 6 | THR | VAL | LEU | GLN | ASP | ARG | GLN | GLU | VAL | LYS | ||||
| 7 | TYR | GLY | LEU | PRO | VAL | LYS | PHE | VAL | ILE | PRO | ||||
| 8 | GLU | ILE | SER | PRO | GLY | ILE | ILE | PHE | THR | GLY | ||||
| 9 | THR | PRO | ILE | GLU | ILE | GLU | TYR | ALA | LYS | LYS | ||||
| 10 | PRO | ASN | CYS | ALA | LYS | SER | SER | LYS | TRP | LEU | ||||
| 11 | VAL | PHE | VAL | ASP | ASN | VAL | ILE | GLN | LYS | ALA | ||||
| 12 | CYS | VAL | GLY | ILE | GLY | GLY | PRO | GLU | ASN | TYR | ||||
| 13 | PRO | GLY | VAL | GLN | THR | LEU | SER | GLY | LEU | PHE | ||||
| 14 | LYS | ILE | GLU | LYS | HIS | GLU | SER | GLY | PHE | GLY | ||||
| 15 | TYR | LYS | LEU | GLY | PHE | CYS | VAL | LYS | GLY | SER | ||||
| 16 | PRO | THR | CYS | LEU | ASP | VAL | GLY | ARG | PHE | ASP | ||||
| 17 | ASN | ASP | GLU | ASP | GLY | ARG | ARG | LEU | ASN | LEU | ||||
| 18 | THR | GLU | HIS | GLU | SER | PHE | GLN | VAL | VAL | PHE | ||||
| 19 | ILE | GLN | ALA | GLU | ALA | ASN | ASP | ALA | GLU | PHE | ||||
| 20 | ILE | LYS | SER | VAL | VAL |
Samples:
sample_1: pea alfa-L-fucosidase, [U-99% 13C; U-99% 15N; U-85% 2H], 0.8 – 1.0 mM
cond_1: pH: 7.7; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
- Bruker Avance 800 MHz
Download simulated HSQC data in one of the following formats:
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