BMRB Entry 5244
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5244
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Title: Backbone Assignment of Endonuclease V from Bacteriophage T4 with deuterium labeling PubMed: 12018491
Deposition date: 2001-12-26 Original release date: 2002-08-22
Authors: Ahn, Hee-Chul; Lee, Sung-Yun; Lee, Bong-Jin
Citation: Ahn, Hee-Chul; Lee, Sung-Yun; Lee, Bong-Jin. "Letter to the Editor: Backbone Assignments for Endonuclease V from Bacteriophage T4 with deuterium labeling " J. Biomol. NMR 22, 383-384 (2002).
Assembly members:
endonuclease V, polymer, 138 residues, Formula weight is not available
Natural source: Common Name: T4 Taxonomy ID: 10665 Superkingdom: Viruses Kingdom: not available Genus/species: T4-like phages Bacteriophage T4
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
endonuclease V: MTRINLTLVSELADQHLMAE
YRELPRVFGAVRKHVANGKR
VRDFKISPTFILGAGHVTFF
YDKLEFLRKRQIELIAECLK
RGFNIKDTTVQDISDIPQEF
RGDYIPHEASIAISQARLDE
KIAQRPTWYKYYGKAIYA
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 253 |
| 13C chemical shifts | 395 |
| 15N chemical shifts | 131 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | endonuclease V | 1 |
Entities:
Entity 1, endonuclease V 138 residues - Formula weight is not available
| 1 | MET | THR | ARG | ILE | ASN | LEU | THR | LEU | VAL | SER | ||||
| 2 | GLU | LEU | ALA | ASP | GLN | HIS | LEU | MET | ALA | GLU | ||||
| 3 | TYR | ARG | GLU | LEU | PRO | ARG | VAL | PHE | GLY | ALA | ||||
| 4 | VAL | ARG | LYS | HIS | VAL | ALA | ASN | GLY | LYS | ARG | ||||
| 5 | VAL | ARG | ASP | PHE | LYS | ILE | SER | PRO | THR | PHE | ||||
| 6 | ILE | LEU | GLY | ALA | GLY | HIS | VAL | THR | PHE | PHE | ||||
| 7 | TYR | ASP | LYS | LEU | GLU | PHE | LEU | ARG | LYS | ARG | ||||
| 8 | GLN | ILE | GLU | LEU | ILE | ALA | GLU | CYS | LEU | LYS | ||||
| 9 | ARG | GLY | PHE | ASN | ILE | LYS | ASP | THR | THR | VAL | ||||
| 10 | GLN | ASP | ILE | SER | ASP | ILE | PRO | GLN | GLU | PHE | ||||
| 11 | ARG | GLY | ASP | TYR | ILE | PRO | HIS | GLU | ALA | SER | ||||
| 12 | ILE | ALA | ILE | SER | GLN | ALA | ARG | LEU | ASP | GLU | ||||
| 13 | LYS | ILE | ALA | GLN | ARG | PRO | THR | TRP | TYR | LYS | ||||
| 14 | TYR | TYR | GLY | LYS | ALA | ILE | TYR | ALA |
Samples:
sample_1: endonuclease V, [U-15N]-Ala, Asp, Arg, Gly, Lys, Ser, Thr, Tyr, Val, 1.0 mM
sample_2: endonuclease V, [U-15N], 2.0 mM
sample_3: endonuclease V, [U-70% 2H ; U-15N], 1.5 mM
sample_4: endonuclease V, [U-90% 2H; U-13C; U-15N], 1.5 mM
Ex-cond: pH: 6.5; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | not available | not available | not available |
| 3D 1H-15N NOESY | not available | not available | not available |
| 3D 1H-15N TOCSY | not available | not available | not available |
| 3D HNHA | not available | not available | not available |
| 3D HNCA | not available | not available | not available |
| 3D HN(CO)CA | not available | not available | not available |
| 3D HNCACB | not available | not available | not available |
| 3D HN(CO)CACB | not available | not available | not available |
| 3D HNCO | not available | not available | not available |
Software:
NMRPipe - data processing
NMRDraw - data processing
NMRView - peak assignments
NMR spectrometers:
- Bruker DRX 500 MHz
- Bruker DRX 600 MHz
Related Database Links:
| PDB | |
| EMBL | CAA28215 |
| GB | AAA72721 AAC42096 AAD42563 ABX11097 ACL78337 |
| REF | NP_049733 YP_001595242 YP_002922460 YP_003734258 |
| SP | P04418 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts