BMRB Entry 5619
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5619
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Title: Resonance assignments for the methionine sulfoxide reductase B from Bacillus subtilis, Northeast Structural Genomics Consortium target SR10
Deposition date: 2002-12-09 Original release date: 2002-12-27
Authors: Zheng, Deyou; Cort, John; Chiang, Yiwen; Kennedy, Michael; Montelione, Gaetano
Citation: Zheng, Deyou; Cort, John; Chiang, Yiwen; Acton, Thomas; Kennedy, Michael; Montelione, Gaetano. "Letter to the Editor: 1H, 13C and 15N resonance assignments for methionine sulfoxide reductase B from Bacillus subtilis" J. Biomol. NMR 27, 183-184 (2003).
Assembly members:
methionine sulfoxide reductase msrB, polymer, 151 residues, Formula weight is not available
Natural source: Common Name: Bacillus subtilis Taxonomy ID: 1423 Superkingdom: Eubacteria Kingdom: not available Genus/species: Bacillus subtilis
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
methionine sulfoxide reductase msrB: MAYNKEEKIKSLNRMQYEVT
QNNGTEPPFQNEYWDHKEEG
LYVDIVSGKPLFTSKDKFDS
QCGWPSFTKPIEEEVEEKLD
TSHGMIRTEVRSRTADSHLG
HVFNDGPGPNGLRYCINSAA
LRFVPKHKLKEEGYESYLHL
FNKLEHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 259 |
| 13C chemical shifts | 447 |
| 15N chemical shifts | 136 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Methionine sulfoxide reductase | 1 |
Entities:
Entity 1, Methionine sulfoxide reductase 151 residues - Formula weight is not available
| 1 | MET | ALA | TYR | ASN | LYS | GLU | GLU | LYS | ILE | LYS | ||||
| 2 | SER | LEU | ASN | ARG | MET | GLN | TYR | GLU | VAL | THR | ||||
| 3 | GLN | ASN | ASN | GLY | THR | GLU | PRO | PRO | PHE | GLN | ||||
| 4 | ASN | GLU | TYR | TRP | ASP | HIS | LYS | GLU | GLU | GLY | ||||
| 5 | LEU | TYR | VAL | ASP | ILE | VAL | SER | GLY | LYS | PRO | ||||
| 6 | LEU | PHE | THR | SER | LYS | ASP | LYS | PHE | ASP | SER | ||||
| 7 | GLN | CYS | GLY | TRP | PRO | SER | PHE | THR | LYS | PRO | ||||
| 8 | ILE | GLU | GLU | GLU | VAL | GLU | GLU | LYS | LEU | ASP | ||||
| 9 | THR | SER | HIS | GLY | MET | ILE | ARG | THR | GLU | VAL | ||||
| 10 | ARG | SER | ARG | THR | ALA | ASP | SER | HIS | LEU | GLY | ||||
| 11 | HIS | VAL | PHE | ASN | ASP | GLY | PRO | GLY | PRO | ASN | ||||
| 12 | GLY | LEU | ARG | TYR | CYS | ILE | ASN | SER | ALA | ALA | ||||
| 13 | LEU | ARG | PHE | VAL | PRO | LYS | HIS | LYS | LEU | LYS | ||||
| 14 | GLU | GLU | GLY | TYR | GLU | SER | TYR | LEU | HIS | LEU | ||||
| 15 | PHE | ASN | LYS | LEU | GLU | HIS | HIS | HIS | HIS | HIS | ||||
| 16 | HIS |
Samples:
sample_1: methionine sulfoxide reductase msrB, [U-13C; U-15N; U-2H] with Val, Leu, Ile(delta) methyl protonated, 1.0 mM
Ex-cond_1: pH: 6.5 na; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| 1H-13C HSQC | not available | not available | not available |
| HNCO | not available | not available | not available |
| HN(CA)CO | not available | not available | not available |
| HNCACB | not available | not available | not available |
| HN(CO)CACB | not available | not available | not available |
| H(CCCO)NH-TOCSY | not available | not available | not available |
| (H)CC(CO)NH-TOCSY | not available | not available | not available |
Software:
FELIX - process
AutoAssign v1.9 - automated backbone assignments
NMR spectrometers:
- Varian INOVA 600 MHz
- Varian INOVA 750 MHz
Related Database Links:
| BMRB | 17008 |
| PDB | |
| DBJ | BAI85662 BAM52650 BAM58225 GAK79338 |
| EMBL | CAB14086 CCU58667 CEI57380 CEJ77805 |
| GB | AAA96648 ADV92894 AEP91184 AFQ58114 AGA23472 |
| REF | NP_390051 WP_003230813 WP_014477116 WP_014480061 WP_021481498 |
| SP | P54155 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts