BMRB Entry 5626
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5626
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Title: 1H 13C and 15N backbone resonance assignments of the N-terminal domain of Drosophila GCM protein
Deposition date: 2002-12-18 Original release date: 2003-06-10
Authors: Shimizu, Masato
Citation: Shimizu, Masato; Hiroaki, Hidekazu; Kohda, Daisuke; Morita, Eugene; Hotta, Shigeki; Morikawa, Kosuke. "Letter to the editor: 1H 13C and 15N backbone resonance assignments of the N-terminal domain of Drosophila GCM protein" J. Biomol. NMR 26, 277-278 (2003).
Assembly members:
glial cell missing, polymer, 159 residues, Formula weight is not available
Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
glial cell missing: GPATKSRVAIDWDINDSKMP
SVGEFDDFNDWSNGHCRLIY
SVQSDEARKHASGWAMRNTN
NHNVNILKKSCLGVLLCSAK
CKLPNGASVHLRPAICDKAR
RKQQGKQCPNRNCNGRLEIQ
ACRGHCGYPVTHFWRRDGNG
IYFQAKGTHDHPRPEAKGS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 318 |
| 15N chemical shifts | 145 |
| 1H chemical shifts | 145 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | GCM monomer | 1 |
Entities:
Entity 1, GCM monomer 159 residues - Formula weight is not available
| 1 | GLY | PRO | ALA | THR | LYS | SER | ARG | VAL | ALA | ILE | ||||
| 2 | ASP | TRP | ASP | ILE | ASN | ASP | SER | LYS | MET | PRO | ||||
| 3 | SER | VAL | GLY | GLU | PHE | ASP | ASP | PHE | ASN | ASP | ||||
| 4 | TRP | SER | ASN | GLY | HIS | CYS | ARG | LEU | ILE | TYR | ||||
| 5 | SER | VAL | GLN | SER | ASP | GLU | ALA | ARG | LYS | HIS | ||||
| 6 | ALA | SER | GLY | TRP | ALA | MET | ARG | ASN | THR | ASN | ||||
| 7 | ASN | HIS | ASN | VAL | ASN | ILE | LEU | LYS | LYS | SER | ||||
| 8 | CYS | LEU | GLY | VAL | LEU | LEU | CYS | SER | ALA | LYS | ||||
| 9 | CYS | LYS | LEU | PRO | ASN | GLY | ALA | SER | VAL | HIS | ||||
| 10 | LEU | ARG | PRO | ALA | ILE | CYS | ASP | LYS | ALA | ARG | ||||
| 11 | ARG | LYS | GLN | GLN | GLY | LYS | GLN | CYS | PRO | ASN | ||||
| 12 | ARG | ASN | CYS | ASN | GLY | ARG | LEU | GLU | ILE | GLN | ||||
| 13 | ALA | CYS | ARG | GLY | HIS | CYS | GLY | TYR | PRO | VAL | ||||
| 14 | THR | HIS | PHE | TRP | ARG | ARG | ASP | GLY | ASN | GLY | ||||
| 15 | ILE | TYR | PHE | GLN | ALA | LYS | GLY | THR | HIS | ASP | ||||
| 16 | HIS | PRO | ARG | PRO | GLU | ALA | LYS | GLY | SER |
Samples:
sample_1: glial cell missing, [U-99% 13C; U-99% 15N], 0.6 mM; potasium phosptae buffer 20 mM; KCl 0.3 M; Glycerol 10%
condition_number_1: ionic strength: 0.66 M; pH*: 6.5; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N NOESY | not available | not available | not available |
| 1H-15N TOCSY | not available | not available | not available |
| HNCA | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
| CBCACONH | not available | not available | not available |
| HNCACB | not available | not available | not available |
| HN(CA)CO | not available | not available | not available |
| HNCO | not available | not available | not available |
Software:
NMRPipe vrelease 2002 -
NMR spectrometers:
- Bruker DRX 500 MHz
- Bruker DMX 600 MHz
- Bruker DMX 750 MHz
Related Database Links:
| DBJ | BAA10905 |
| GB | AAC46912 AAC47808 AAF52790 ABK30923 AGB92827 |
| REF | NP_001260292 NP_477108 XP_001969320 XP_002036350 XP_002088946 |
| SP | Q27403 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts