BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5658

Title: Sequence specific backbone assignment of 13C, 2H, 15N labeled ISG15

Deposition date: 2003-01-13 Original release date: 2003-02-25

Authors: Yin, Cuifeng; Cort, John; Swapna, G.; Krug, Robert; Kennedy, Michael; Montelione, Gaetano

Citation: Yin, Cuifeng; Aramini, J.; Ma, L.-C.; Cort, John; Swapna, G.; Krug, Robert; Montelione, Gaetano. "Backbone and Ile-delta1, Leu, Val methyl 1H, 13C, and 15N NMR chemical shift assignments for human interferon-stimulated gene 15 protein"  Biomol. NMR Assign. ., .-..

Assembly members:
ISG15, polymer, 164 residues, 18055.6 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
ISG15: SHHHHHHMGWDLTVKMLAGN EFQVSLSSSMSVSELKAQIT QKIGVHAFQQRLAVHPSGVA LQDRVPLASQGLGPGSTVLL VVDKCDEPLSILVRNNKGRS STYEVRLTQTVAHLKQQVSG LEGVQDDLFWLTFEGKPLED QLPLGEYGLKPLSTVFMNLR LRGG

Data sets:
Data typeCount
1H chemical shifts399
13C chemical shifts530
15N chemical shifts162

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ISG15 monomer1

Entities:

Entity 1, ISG15 monomer 164 residues - 18055.6 Da.

1   SERHISHISHISHISHISHISMETGLYTRP
2   ASPLEUTHRVALLYSMETLEUALAGLYASN
3   GLUPHEGLNVALSERLEUSERSERSERMET
4   SERVALSERGLULEULYSALAGLNILETHR
5   GLNLYSILEGLYVALHISALAPHEGLNGLN
6   ARGLEUALAVALHISPROSERGLYVALALA
7   LEUGLNASPARGVALPROLEUALASERGLN
8   GLYLEUGLYPROGLYSERTHRVALLEULEU
9   VALVALASPLYSCYSASPGLUPROLEUSER
10   ILELEUVALARGASNASNLYSGLYARGSER
11   SERTHRTYRGLUVALARGLEUTHRGLNTHR
12   VALALAHISLEULYSGLNGLNVALSERGLY
13   LEUGLUGLYVALGLNASPASPLEUPHETRP
14   LEUTHRPHEGLUGLYLYSPROLEUGLUASP
15   GLNLEUPROLEUGLYGLUTYRGLYLEULYS
16   PROLEUSERTHRVALPHEMETASNLEUARG
17   LEUARGGLYGLY

Samples:

sample_1: Interferon-Stimulated gene 15 product, [U-70% 2H; U-100% 13C; U-100% 15N], 0.7 mM; ammonium citrate 50 mM; CaCl2 5 mM; DTT 10 mM

condition_1: pH: 6.5; temperature: 291 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYsample_1not availablecondition_1
HNCAsample_1not availablecondition_1
HN(CO)CAsample_1not availablecondition_1
HNCOsample_1not availablecondition_1
HN(CA)COsample_1not availablecondition_1
HNCACBsample_1not availablecondition_1
HN(CO)CACBsample_1not availablecondition_1
HSQCsample_1not availablecondition_1

Software:

AUTOASSIGN v1.10.0 - automated peak assignments

NMR spectrometers:

  • Varian INOVA 750 MHz

Related Database Links:

PDB
DBJ BAJ20347
GB AAA36038 AAA36128 AAH09507 AAN86983 AAP35961
REF NP_005092 XP_003806031 XP_009452953 XP_520842
SP P05161

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts