BMRB Entry 5736
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5736
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Title: Sequence specific resonance assignments of ICln, an ion channel cloned from epithelial cells PubMed: 14512740
Deposition date: 2003-03-14 Original release date: 2003-10-08
Authors: Schedlbauer, Andreas; Kontaxis, Georg; Konig, Matthias; Furst, Johannes; Botta, Guido; Paulmichl, Markus; Konrat, Robert
Citation: Schedlbauer, Andreas; Kontaxis, Georg; Konig, Matthias; Furst, Johannes; Jakab, Martin; Ritter, Markus; Garavaglia, Lisa; Botta, Guido; Meyer, Giuliano; Paulmichl, Markus; Konrat, Robert. "Letter to the Editor: Sequence-specific Resonance Assignments of ICln, an ion channel cloned from epithelial Cells " J. Biomol. NMR 27, 399-400 (2003).
Assembly members:
ICln, polymer, 244 residues, Formula weight is not available
Natural source: Common Name: Dog Taxonomy ID: 9615 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Canis familiaris
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
ICln: MHHHHHHLEMSFLKSFPPPG
SAEGLRQQQPETEAVLNGKG
LGTGTLYIAESRLSWLDGSG
LGFSLEYPTISLHAVSRDLN
AYPREHLYVMVNAKFGEESK
ESVAEEEDSDDDVEPIAEFR
FVPSDKSALEAMFTAMCECQ
ALHPDPEDEDSDDYDGEEYD
VEAHEQGQGDIPTFYTYEEG
LSHLTAEGQATLERLEGMLS
QSVSSQYNMAGVRTEDSTRD
YEDGMEVDTTPTVAGQFEDA
DVDH
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 171 |
| 1H chemical shifts | 175 |
| 13C chemical shifts | 553 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ICln monomer | 1 |
Entities:
Entity 1, ICln monomer 244 residues - Formula weight is not available
| 1 | MET | HIS | HIS | HIS | HIS | HIS | HIS | LEU | GLU | MET | ||||
| 2 | SER | PHE | LEU | LYS | SER | PHE | PRO | PRO | PRO | GLY | ||||
| 3 | SER | ALA | GLU | GLY | LEU | ARG | GLN | GLN | GLN | PRO | ||||
| 4 | GLU | THR | GLU | ALA | VAL | LEU | ASN | GLY | LYS | GLY | ||||
| 5 | LEU | GLY | THR | GLY | THR | LEU | TYR | ILE | ALA | GLU | ||||
| 6 | SER | ARG | LEU | SER | TRP | LEU | ASP | GLY | SER | GLY | ||||
| 7 | LEU | GLY | PHE | SER | LEU | GLU | TYR | PRO | THR | ILE | ||||
| 8 | SER | LEU | HIS | ALA | VAL | SER | ARG | ASP | LEU | ASN | ||||
| 9 | ALA | TYR | PRO | ARG | GLU | HIS | LEU | TYR | VAL | MET | ||||
| 10 | VAL | ASN | ALA | LYS | PHE | GLY | GLU | GLU | SER | LYS | ||||
| 11 | GLU | SER | VAL | ALA | GLU | GLU | GLU | ASP | SER | ASP | ||||
| 12 | ASP | ASP | VAL | GLU | PRO | ILE | ALA | GLU | PHE | ARG | ||||
| 13 | PHE | VAL | PRO | SER | ASP | LYS | SER | ALA | LEU | GLU | ||||
| 14 | ALA | MET | PHE | THR | ALA | MET | CYS | GLU | CYS | GLN | ||||
| 15 | ALA | LEU | HIS | PRO | ASP | PRO | GLU | ASP | GLU | ASP | ||||
| 16 | SER | ASP | ASP | TYR | ASP | GLY | GLU | GLU | TYR | ASP | ||||
| 17 | VAL | GLU | ALA | HIS | GLU | GLN | GLY | GLN | GLY | ASP | ||||
| 18 | ILE | PRO | THR | PHE | TYR | THR | TYR | GLU | GLU | GLY | ||||
| 19 | LEU | SER | HIS | LEU | THR | ALA | GLU | GLY | GLN | ALA | ||||
| 20 | THR | LEU | GLU | ARG | LEU | GLU | GLY | MET | LEU | SER | ||||
| 21 | GLN | SER | VAL | SER | SER | GLN | TYR | ASN | MET | ALA | ||||
| 22 | GLY | VAL | ARG | THR | GLU | ASP | SER | THR | ARG | ASP | ||||
| 23 | TYR | GLU | ASP | GLY | MET | GLU | VAL | ASP | THR | THR | ||||
| 24 | PRO | THR | VAL | ALA | GLY | GLN | PHE | GLU | ASP | ALA | ||||
| 25 | ASP | VAL | ASP | HIS |
Samples:
sample_1: ICln mM
sample_conditions: pH: 7.0; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | sample_1 | not available | sample_conditions |
| 3D HNCO | sample_1 | not available | sample_conditions |
| 3D HNCACB | sample_1 | not available | sample_conditions |
| 3D CBCACONH | sample_1 | not available | sample_conditions |
Software:
No software information available
NMR spectrometers:
- Varian INOVA 800 MHz
Related Database Links:
| PDB | |
| EMBL | CAA46447 |
| GB | EFB16643 |
| PRF | 1808296A |
| REF | NP_001003288 XP_002916314 XP_003992740 XP_004391878 XP_004781086 |
| SP | P35521 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts