BMRB Entry 5774
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5774
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Title: Recognition of GU-rich polyadenylation regulatory elements by human CstF-64 protein PubMed: 12773396
Deposition date: 2003-04-15 Original release date: 2003-08-01
Authors: Perez-Canadillas, Jose; Varani, Gabriele
Citation: Perez-Canadillas, Jose; Varani, Gabriele. "Recognition of GU-rich Polyadenylation Regulatory Elements by Human CstF-64 Protein" EMBO J. 22, 2821-2830 (2003).
Assembly members:
Cleavage stimulation Factor 64, RRM domain, polymer, 111 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9660 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Cleavage stimulation Factor 64, RRM domain: MAGLTVRDPAVDRSLRSVFV
GNIPYEATEEQLKDIFSEVG
PVVSFRLVYDRETGKPKGYG
FCEYQDQETALSAMRNLNGR
EFSGRALRVDNAASEKNKEE
LKSLGTGAPVI
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 385 |
| 15N chemical shifts | 112 |
| 1H chemical shifts | 603 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CstF64 RRM domain | 1 |
Entities:
Entity 1, CstF64 RRM domain 111 residues - Formula weight is not available
| 1 | MET | ALA | GLY | LEU | THR | VAL | ARG | ASP | PRO | ALA | ||||
| 2 | VAL | ASP | ARG | SER | LEU | ARG | SER | VAL | PHE | VAL | ||||
| 3 | GLY | ASN | ILE | PRO | TYR | GLU | ALA | THR | GLU | GLU | ||||
| 4 | GLN | LEU | LYS | ASP | ILE | PHE | SER | GLU | VAL | GLY | ||||
| 5 | PRO | VAL | VAL | SER | PHE | ARG | LEU | VAL | TYR | ASP | ||||
| 6 | ARG | GLU | THR | GLY | LYS | PRO | LYS | GLY | TYR | GLY | ||||
| 7 | PHE | CYS | GLU | TYR | GLN | ASP | GLN | GLU | THR | ALA | ||||
| 8 | LEU | SER | ALA | MET | ARG | ASN | LEU | ASN | GLY | ARG | ||||
| 9 | GLU | PHE | SER | GLY | ARG | ALA | LEU | ARG | VAL | ASP | ||||
| 10 | ASN | ALA | ALA | SER | GLU | LYS | ASN | LYS | GLU | GLU | ||||
| 11 | LEU | LYS | SER | LEU | GLY | THR | GLY | ALA | PRO | VAL | ||||
| 12 | ILE |
Samples:
sample_1: Cleavage stimulation Factor 64, RRM domain, [U-99% 13C; U-99% 15N], 1.0 mM
cond_set_1: ionic strength: 0.02 M; pH: 6.0; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | not available | not available | not available |
| 1H-15N HSQC-NOESY | not available | not available | not available |
| 1H-13C HSQC-NOESY | not available | not available | not available |
| 15N HSQC | not available | not available | not available |
| 13C HSQC | not available | not available | not available |
| HNCA | not available | not available | not available |
| HNCO | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
| CBCA(CO)HN | not available | not available | not available |
| HCCH-COSY | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- unknown unknown 0 MHz
Related Database Links:
| PDB | |
| DBJ | BAC28037 BAC40243 BAG62297 BAI46582 |
| EMBL | CAG09844 CAG31323 CAH90254 CDQ70066 CDQ72626 |
| GB | AAA35724 AAB50269 AAG31814 AAH17712 AAH33135 |
| REF | NP_001006433 NP_001080179 NP_001123707 NP_001124486 NP_001125111 |
| SP | P33240 Q5RDA3 Q8BIQ5 Q8HXM1 |
| TPG | DAA13112 |
Download simulated HSQC data in one of the following formats:
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