BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5802

Title: 1H, 13C and 15N Backbone Resonance assignments for the water soluble domain of ferric rat liver outer mitochondrial membrane cytochrome b5   PubMed: 15981997

Deposition date: 2003-05-19 Original release date: 2007-03-22

Authors: Massiah, Michael; Altuve, Adriana; Eastman, Margaret; Caignan, Gregori; Rivera, Mario

Citation: Simeonov, M.; Altuve, Adriana; Massiah, Michael; Wang, A.; Eastman, Margaret; Benson, D.; Rivera, Mario. "Mitochondrial and microsomal ferric b5 cytochromes exhibit divergent conformational plasticity in the context of a common fold"  Biochemistry 44, 9308-9319 (2005).

Assembly members:
membrane of mitochondria cytochrome b5, polymer, 93 residues, 11000 Da.
HEM, non-polymer, 616.487 Da.

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
membrane of mitochondria cytochrome b5: MDGQGSDPAVTYYRLEEVAK RNTAEETWMVIHGRVYDITR FLSEHPGGEEVLLEQAGADA TESFEDVGHSPDAREMLKQY YIGDVHPNDLKPK

Data sets:
Data typeCount
1H chemical shifts242
13C chemical shifts359
15N chemical shifts120

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1omb5 isomer 11
2omb5 isomer 21
3Heme2

Entities:

Entity 1, omb5 isomer 1 93 residues - 11000 Da.

1   METASPGLYGLNGLYSERASPPROALAVAL
2   THRTYRTYRARGLEUGLUGLUVALALALYS
3   ARGASNTHRALAGLUGLUTHRTRPMETVAL
4   ILEHISGLYARGVALTYRASPILETHRARG
5   PHELEUSERGLUHISPROGLYGLYGLUGLU
6   VALLEULEUGLUGLNALAGLYALAASPALA
7   THRGLUSERPHEGLUASPVALGLYHISSER
8   PROASPALAARGGLUMETLEULYSGLNTYR
9   TYRILEGLYASPVALHISPROASNASPLEU
10   LYSPROLYS

Entity 2, Heme - C34 H32 Fe N4 O4 - 616.487 Da.

1   HEM

Samples:

sample_1: membrane of mitochondria cytochrome b5, [U-99% 15N; U-99% 13C], 1.0 mM; PROTOPORPHYRIN IX CONTAINING FE 1.0 mM; Na2HPO4 0.0268 M; NaH2PO4 0.0226 M

cond_1: pH: 7.0; temperature: 303 K; ionic strength: 0.1 M; pressure: 760 mmHg

Experiments:

NameSampleSample stateSample conditions
2D-1H-15N HSQCsample_1not availablecond_1
3D-1H-15N NOESY-HSQCsample_1not availablecond_1
3D-1H-15N HMQC-NOESY-HSQCsample_1not availablecond_1
HNCAsample_1not availablecond_1
CBCACONHsample_1not availablecond_1
3D-HNHAsample_1not availablecond_1
HNCOsample_1not availablecond_1
HNCACBsample_1not availablecond_1

Software:

NMRpipe - data processing

Sparky v3.0 - data analysis

NMR spectrometers:

  • Varian Inova 600 MHz

Related Database Links:

PDB
DBJ BAE38844
EMBL CAA65256 CAA73117
GB AAH72535 EDL92471
REF NP_085075
SP P04166

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts