BMRB Entry 5853
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5853
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Title: Sequential 1H, 15N and 13C NMR assignment of human Calbindin D28k PubMed: 14752267
Deposition date: 2003-06-30 Original release date: 2004-02-11
Authors: Helgstrand, Magnus; Vanbelle, Christophe; Thulin, Eva; Linse, Sara; Akke, Mikael
Citation: Helgstrand, Magnus; Vanbelle, Christophe; Thulin, Eva; Linse, Sara; Akke, Mikael. "Letter to the Editor: Sequential 1H, 15N and 13C NMR assignment of human Calbindin D28k" J. Biomol. NMR 28, 305-306 (2004).
Assembly members:
Calbindin D28k, polymer, 261 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Calbindin D28k: MEASHLQSSLITASQFFEIW
LHFDADGSGYLEGKELQNLI
QELQQARKKAGLELSPEMKT
FVDQYGQRDDGKIGIVELAH
VLPTEENFLLLFRSQQLKSS
EEFMKTWRKYDTDHSGFIET
EELKNFLKDLLEKANKTVDD
TKLAEYTDLMLKLFDSNNDG
KLELTEMARLLPVQENFLLK
FEGIKMSGKEFNKAFELYDQ
DGDGYIDENELDALLKDLSE
KNKQDLDINNITTYKKNIMA
LSDGGKLYRTDLALILSAGD
N
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 706 |
| 15N chemical shifts | 240 |
| 1H chemical shifts | 240 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Calbindin D28k | 1 |
| 2 | CALCIUM (II) ION, 1 | 2 |
| 3 | CALCIUM (II) ION, 2 | 2 |
| 4 | CALCIUM (II) ION, 3 | 2 |
| 5 | CALCIUM (II) ION, 4 | 2 |
Entities:
Entity 1, Calbindin D28k 261 residues - Formula weight is not available
| 1 | MET | GLU | ALA | SER | HIS | LEU | GLN | SER | SER | LEU | ||||
| 2 | ILE | THR | ALA | SER | GLN | PHE | PHE | GLU | ILE | TRP | ||||
| 3 | LEU | HIS | PHE | ASP | ALA | ASP | GLY | SER | GLY | TYR | ||||
| 4 | LEU | GLU | GLY | LYS | GLU | LEU | GLN | ASN | LEU | ILE | ||||
| 5 | GLN | GLU | LEU | GLN | GLN | ALA | ARG | LYS | LYS | ALA | ||||
| 6 | GLY | LEU | GLU | LEU | SER | PRO | GLU | MET | LYS | THR | ||||
| 7 | PHE | VAL | ASP | GLN | TYR | GLY | GLN | ARG | ASP | ASP | ||||
| 8 | GLY | LYS | ILE | GLY | ILE | VAL | GLU | LEU | ALA | HIS | ||||
| 9 | VAL | LEU | PRO | THR | GLU | GLU | ASN | PHE | LEU | LEU | ||||
| 10 | LEU | PHE | ARG | SER | GLN | GLN | LEU | LYS | SER | SER | ||||
| 11 | GLU | GLU | PHE | MET | LYS | THR | TRP | ARG | LYS | TYR | ||||
| 12 | ASP | THR | ASP | HIS | SER | GLY | PHE | ILE | GLU | THR | ||||
| 13 | GLU | GLU | LEU | LYS | ASN | PHE | LEU | LYS | ASP | LEU | ||||
| 14 | LEU | GLU | LYS | ALA | ASN | LYS | THR | VAL | ASP | ASP | ||||
| 15 | THR | LYS | LEU | ALA | GLU | TYR | THR | ASP | LEU | MET | ||||
| 16 | LEU | LYS | LEU | PHE | ASP | SER | ASN | ASN | ASP | GLY | ||||
| 17 | LYS | LEU | GLU | LEU | THR | GLU | MET | ALA | ARG | LEU | ||||
| 18 | LEU | PRO | VAL | GLN | GLU | ASN | PHE | LEU | LEU | LYS | ||||
| 19 | PHE | GLU | GLY | ILE | LYS | MET | SER | GLY | LYS | GLU | ||||
| 20 | PHE | ASN | LYS | ALA | PHE | GLU | LEU | TYR | ASP | GLN | ||||
| 21 | ASP | GLY | ASP | GLY | TYR | ILE | ASP | GLU | ASN | GLU | ||||
| 22 | LEU | ASP | ALA | LEU | LEU | LYS | ASP | LEU | SER | GLU | ||||
| 23 | LYS | ASN | LYS | GLN | ASP | LEU | ASP | ILE | ASN | ASN | ||||
| 24 | ILE | THR | THR | TYR | LYS | LYS | ASN | ILE | MET | ALA | ||||
| 25 | LEU | SER | ASP | GLY | GLY | LYS | LEU | TYR | ARG | THR | ||||
| 26 | ASP | LEU | ALA | LEU | ILE | LEU | SER | ALA | GLY | ASP | ||||
| 27 | ASN |
Entity 2, CALCIUM (II) ION, 1 - Ca - 40.078 Da.
| 1 | CA |
Samples:
sample_1: Calbindin D28k, [U-13C; U-15N], 1.0 mM; CaCl2 11.0 mM; Mes-buffer 20.0 mM; EDTA 1.0 mM; NaN3 0.02%
sample_2: Calbindin D28k, [U-15N], 2.0 mM; Mes-buffer 20.0 mM; CaCl2 11.0 mM; NaN3 0.02%
Exp_condition_1: pH: 6.5; temperature: 323 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | Exp_condition_1 |
| HNCA | not available | not available | Exp_condition_1 |
| HNCACB | not available | not available | Exp_condition_1 |
| CBCA(CO)NH | not available | not available | Exp_condition_1 |
| HNCO | not available | not available | Exp_condition_1 |
| (HA)CO(CA)NH | not available | not available | Exp_condition_1 |
| C(CO)NH-TOCSY | not available | not available | Exp_condition_1 |
| H(CCO)NH-TOCSY | not available | not available | Exp_condition_1 |
| 3D 1H-15N NOESY | not available | not available | Exp_condition_1 |
Software:
NMRPipe - data processing
Ansig for Windows - assignment
NMR spectrometers:
- Varian Inova 600 MHz
- Varian Inova 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts