BMRB Entry 5863
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5863
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Title: Backbone HN, N, Ca, C and Cb chemical shift assignments and Secondary Structure of FkpA, a 245-residue peptidyl- prolyl cis/trans isomerase with chaperone activity PubMed: 14872134
Deposition date: 2003-07-08 Original release date: 2004-02-13
Authors: Hu, Kaifeng; Pluckthun, Andreas; Pervushin, Konstantin
Citation: Hu, Kaifeng; Pluckthun, Andreas; Pervushin, Konstantin. "Letter to the Editor: Backbone HN, N, CA, C' and CB chemical shift assignments and Secondary Structure of FkpA, a 245-residue peptidyl- prolyl cis/trans isomerase with chaperone activity " J. Biomol. NMR 28, 405-406 (2004).
Assembly members:
FkpA, polymer, 245 residues, 26223 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
FkpA: AEAAKPATAADSKAAFKNDD
QKSAYALGASLGRYMENSLK
EQEKLGIKLDKDQLIAGVQD
AFADKSKLSDQEIEQTLQAF
EARVKSSAQAKMEKDAADNE
AKGKEYREKFAKEKGVKTSS
TGLVYQVVEAGKGEAPKDSD
TVVVNYKGTLIDGKEFDNSY
TRGEPLSFRLDGVIPGWTEG
LKNIKKGGKIKLVIPPELAY
GKAGVPGIPPNSTLVFDVEL
LDVKPAPKADAKPEADAKAA
DSAKK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 219 |
| 13C chemical shifts | 685 |
| 15N chemical shifts | 219 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | FkpA, chain 1 | 1 |
| 2 | FkpA, chain 2 | 1 |
Entities:
Entity 1, FkpA, chain 1 245 residues - 26223 Da.
| 1 | ALA | GLU | ALA | ALA | LYS | PRO | ALA | THR | ALA | ALA | ||||
| 2 | ASP | SER | LYS | ALA | ALA | PHE | LYS | ASN | ASP | ASP | ||||
| 3 | GLN | LYS | SER | ALA | TYR | ALA | LEU | GLY | ALA | SER | ||||
| 4 | LEU | GLY | ARG | TYR | MET | GLU | ASN | SER | LEU | LYS | ||||
| 5 | GLU | GLN | GLU | LYS | LEU | GLY | ILE | LYS | LEU | ASP | ||||
| 6 | LYS | ASP | GLN | LEU | ILE | ALA | GLY | VAL | GLN | ASP | ||||
| 7 | ALA | PHE | ALA | ASP | LYS | SER | LYS | LEU | SER | ASP | ||||
| 8 | GLN | GLU | ILE | GLU | GLN | THR | LEU | GLN | ALA | PHE | ||||
| 9 | GLU | ALA | ARG | VAL | LYS | SER | SER | ALA | GLN | ALA | ||||
| 10 | LYS | MET | GLU | LYS | ASP | ALA | ALA | ASP | ASN | GLU | ||||
| 11 | ALA | LYS | GLY | LYS | GLU | TYR | ARG | GLU | LYS | PHE | ||||
| 12 | ALA | LYS | GLU | LYS | GLY | VAL | LYS | THR | SER | SER | ||||
| 13 | THR | GLY | LEU | VAL | TYR | GLN | VAL | VAL | GLU | ALA | ||||
| 14 | GLY | LYS | GLY | GLU | ALA | PRO | LYS | ASP | SER | ASP | ||||
| 15 | THR | VAL | VAL | VAL | ASN | TYR | LYS | GLY | THR | LEU | ||||
| 16 | ILE | ASP | GLY | LYS | GLU | PHE | ASP | ASN | SER | TYR | ||||
| 17 | THR | ARG | GLY | GLU | PRO | LEU | SER | PHE | ARG | LEU | ||||
| 18 | ASP | GLY | VAL | ILE | PRO | GLY | TRP | THR | GLU | GLY | ||||
| 19 | LEU | LYS | ASN | ILE | LYS | LYS | GLY | GLY | LYS | ILE | ||||
| 20 | LYS | LEU | VAL | ILE | PRO | PRO | GLU | LEU | ALA | TYR | ||||
| 21 | GLY | LYS | ALA | GLY | VAL | PRO | GLY | ILE | PRO | PRO | ||||
| 22 | ASN | SER | THR | LEU | VAL | PHE | ASP | VAL | GLU | LEU | ||||
| 23 | LEU | ASP | VAL | LYS | PRO | ALA | PRO | LYS | ALA | ASP | ||||
| 24 | ALA | LYS | PRO | GLU | ALA | ASP | ALA | LYS | ALA | ALA | ||||
| 25 | ASP | SER | ALA | LYS | LYS |
Samples:
sample_1: FkpA, [U-99% 13C; U-99% 15N; U-98% 2H], 0.6 mM; Mes buffer 20 mM; NaCl 50 mM
sample_2: FkpA, [U-99% 13C; U-99% 15N; U-98% 2H], 0.5 mM; Mes buffer 20 mM; NaCl 20 mM
Ex-cond_1: pH: 6.0; temperature: 310 K; ionic strength: 0.02 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| Bruker Avance 600 MHz spectrometer equipped with a TXI cryogenic probehead. | not available | not available | Ex-cond_1 |
| 3D TROSY-versions of HNCA, HNCACB, HNCO and HN(CA)CO run in the high | not available | not available | Ex-cond_1 |
| resolution mode, utilizing both N->C and C->N polarization transfer periods to | not available | not available | Ex-cond_1 |
| maximize spectral resolution along the 15N dimension. | not available | not available | Ex-cond_1 |
Software:
XEASY -
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz
- Bruker Avance 900 MHz
Related Database Links:
| PDB | |
| DBJ | BAB37621 BAE77944 BAG79132 BAI27605 BAI32775 |
| EMBL | CAP77799 CAQ33666 CAR00285 CAR04951 CAR10001 |
| GB | AAA58144 AAC41459 AAC76372 AAG58454 AAN44828 |
| REF | NP_289894 NP_312225 NP_417806 NP_709121 NP_755985 |
| SP | P45523 P65764 P65765 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts