BMRB Entry 5970

Name: Solution Structure of Ribosomal Protein L18 from Bacillus stearothermophilus
Published: 2004-02-13

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5970

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Solution Structure of Ribosomal Protein L18 from Bacillus stearothermophilus PubMed: 14687565

Deposition date: 2003-10-14 Original release date: 2004-02-13

Authors: Turner, C.; Moore, P.

Citation: Turner, C.; Moore, P.. "The Solution Structure of Ribosomal Protein L18 from Bacillus stearothermophilus " J. Mol. Biol. 335, 679-684 (2004).

Assembly members:
Bacillus stearothermophius L18, polymer, 120 residues, Formula weight is not available

Natural source: Common Name: Geobacillus stearothermophilus Taxonomy ID: 1422 Superkingdom: Eubacteria Kingdom: not available Genus/species: Bacillus stearothermophilus

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
Bacillus stearothermophius L18: MITKVDRNAVRKKRHARIRK KIFGTTERPRLSVFRSNKHI YAQIIDDTKSATIVSASTLD KEFGLDSTNNIEAAKKVGEL VAKRALEKGIKQVVFDRGGY LYHGRVKALADAAREAGLEF

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
1H chemical shifts	597
13C chemical shifts	245
15N chemical shifts	114

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	50S Ribosomal Protein L18	1

Entities:

Entity 1, 50S Ribosomal Protein L18 120 residues - Formula weight is not available

1	MET	ILE	THR	LYS	VAL	ASP	ARG	ASN	ALA	VAL
2	ARG	LYS	LYS	ARG	HIS	ALA	ARG	ILE	ARG	LYS
3	LYS	ILE	PHE	GLY	THR	THR	GLU	ARG	PRO	ARG
4	LEU	SER	VAL	PHE	ARG	SER	ASN	LYS	HIS	ILE
5	TYR	ALA	GLN	ILE	ILE	ASP	ASP	THR	LYS	SER
6	ALA	THR	ILE	VAL	SER	ALA	SER	THR	LEU	ASP
7	LYS	GLU	PHE	GLY	LEU	ASP	SER	THR	ASN	ASN
8	ILE	GLU	ALA	ALA	LYS	LYS	VAL	GLY	GLU	LEU
9	VAL	ALA	LYS	ARG	ALA	LEU	GLU	LYS	GLY	ILE
10	LYS	GLN	VAL	VAL	PHE	ASP	ARG	GLY	GLY	TYR
11	LEU	TYR	HIS	GLY	ARG	VAL	LYS	ALA	LEU	ALA
12	ASP	ALA	ALA	ARG	GLU	ALA	GLY	LEU	GLU	PHE

Samples:

sample_1: Bacillus stearothermophius L18, [U-15N], 1.5 mM; phosphate buffer 20 mM; NaCl 200 mM; MgCl2 5 mM; H2O 90%; D2O 10%

sample_2: Bacillus stearothermophius L18, [U-15N; U-13C], 1.5 mM; phosphate buffer 20 mM; NaCl 200 mM; MgCl2 5 mM; H2O 90%; D2O 10%

sample_3: Bacillus stearothermophius L18 1 mM; phosphate buffer 20 mM; NaCl 200 mM; MgCl2 5 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 6.0 na; temperature: 298 K; ionic strength: 225 mM; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	not available	not available	not available
HNCA	not available	not available	not available
HNCACB	not available	not available	not available
HNCO	not available	not available	not available
HNCACO	not available	not available	not available
HCCH-TOCSY	not available	not available	not available
15N TOCSY-HSQC	not available	not available	not available
15N NOESY-HSQC	not available	not available	not available
2D NOESY	not available	not available	not available
13C NOESY-HSQC	not available	not available	not available

Software:

FELIX v97.0 - processing

Sparky v3.93 - data analysis

CNS v1.0 - structure solution, refinement

ARIA v1.2 - structure solution, refinement

NMR spectrometers:

Varian UNITY 600 MHz

PDB	1OVY
DBJ	BAD74407 GAD13888 GAJ56889
GB	AAA22702 ACX76823 ADI25196 AEV17466 AGE20738
REF	WP_011229635 WP_033008681 YP_003251305 YP_003669773 YP_004980566
SP	P09415 Q5L3S3

Biological Magnetic Resonance Data Bank