BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6100

Title: Backbone 1H, 13C and 15N chemical shift assignents of the 18.5kDa isoform of murine myelin basic protein (MBP)   PubMed: 15243191

Deposition date: 2004-02-12 Original release date: 2007-10-19

Authors: Libich, David; Robertson, Valerie; Monette, Martine; Harauz, George

Citation: Libich, David; Robertson, Valerie; Monette, Martine; Harauz, George. "Letter to the Editor: Backbone resonance assignments of the 18.5kDa isoform of murine myelin basic protein (MBP)"  J. Biomol. NMR 29, 545-546 (2004).

Assembly members:
myelin basic protein, polymer, 176 residues, 19421.5 Da.

Natural source:   Common Name: house mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
myelin basic protein: ASQKRPSQRSKYLATASTMD HARHGFLPRHRDTGILDSIG RFFSGDRGAPKRGSGKDSHT RTTHYGSLPQKSQHGRTQDE NPVVHFFKNIVTPRTPPPSQ GKGRGLSLSRFSWGAEGQRP GFGYGGRASDYKSAHKGFKG AYDAQGTLSKIFKLGGRDSR SGSPMARRLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts436
15N chemical shifts154
1H chemical shifts516

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1rmMBP1

Entities:

Entity 1, rmMBP 176 residues - 19421.5 Da.

1   ALASERGLNLYSARGPROSERGLNARGSER
2   LYSTYRLEUALATHRALASERTHRMETASP
3   HISALAARGHISGLYPHELEUPROARGHIS
4   ARGASPTHRGLYILELEUASPSERILEGLY
5   ARGPHEPHESERGLYASPARGGLYALAPRO
6   LYSARGGLYSERGLYLYSASPSERHISTHR
7   ARGTHRTHRHISTYRGLYSERLEUPROGLN
8   LYSSERGLNHISGLYARGTHRGLNASPGLU
9   ASNPROVALVALHISPHEPHELYSASNILE
10   VALTHRPROARGTHRPROPROPROSERGLN
11   GLYLYSGLYARGGLYLEUSERLEUSERARG
12   PHESERTRPGLYALAGLUGLYGLNARGPRO
13   GLYPHEGLYTYRGLYGLYARGALASERASP
14   TYRLYSSERALAHISLYSGLYPHELYSGLY
15   ALATYRASPALAGLNGLYTHRLEUSERLYS
16   ILEPHELYSLEUGLYGLYARGASPSERARG
17   SERGLYSERPROMETALAARGARGLEUGLU
18   HISHISHISHISHISHIS

Samples:

sample_1: myelin basic protein, [U-15N; U-13C], 2 mM; 2,2,2-trifluoroethanol-d2, [2H], 30%

sample_cond_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N-HSQCsample_1not availablesample_cond_1
CBCANHsample_1not availablesample_cond_1
CBCA(CO)NHsample_1not availablesample_cond_1
HCC(CO)NHsample_1not availablesample_cond_1
HNCOsample_1not availablesample_cond_1

Software:

No software information available

NMR spectrometers:

  • Bruker AV 600 MHz

Related Database Links:

BMRB 15131
GB AAA37720 AAA39496 AAA39501 EDL09385
REF NP_001020426 NP_001020463 NP_034907 XP_006526519
SP P04370

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts