BMRB Entry 6193
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6193
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Title: 1H, 13C, 15N PSD-95 PDZ3 NMR Assignments, pH 7, 20C PubMed: 15452444
Deposition date: 2004-04-26 Original release date: 2004-10-01
Authors: Ukpabi, Nkoli; Sharma, Sudhir; Spaller, Mark; Tsang, Pearl
Citation: Ukpabi, Nkoli; Sharma, Sudhir; Spaller, Mark; Tsang, Pearl. "Letter to the Editor: 1H, 15N and 13C Backbone and Side Chain Assignments of PSD-95 PDZ3 protein" J. Biomol. NMR 30, 111-112 (2004).
Assembly members:
PSD-95 PDZ3, polymer, 109 residues, Formula weight is not available
Natural source: Common Name: Norway rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
PSD-95 PDZ3: GSPEFLGEEDIPREPRRIVI
HRGSTGLGFNIVGGEDGEGI
FISFILAGGPADLSGELRKG
DQILSVNGVDLRNASHEQAA
IALKNAGQTVTIIAQYKPEE
YSRFEANSR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 328 |
| 15N chemical shifts | 118 |
| 1H chemical shifts | 580 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PSD-95 PDZ3 | 1 |
Entities:
Entity 1, PSD-95 PDZ3 109 residues - Formula weight is not available
| 1 | GLY | SER | PRO | GLU | PHE | LEU | GLY | GLU | GLU | ASP | ||||
| 2 | ILE | PRO | ARG | GLU | PRO | ARG | ARG | ILE | VAL | ILE | ||||
| 3 | HIS | ARG | GLY | SER | THR | GLY | LEU | GLY | PHE | ASN | ||||
| 4 | ILE | VAL | GLY | GLY | GLU | ASP | GLY | GLU | GLY | ILE | ||||
| 5 | PHE | ILE | SER | PHE | ILE | LEU | ALA | GLY | GLY | PRO | ||||
| 6 | ALA | ASP | LEU | SER | GLY | GLU | LEU | ARG | LYS | GLY | ||||
| 7 | ASP | GLN | ILE | LEU | SER | VAL | ASN | GLY | VAL | ASP | ||||
| 8 | LEU | ARG | ASN | ALA | SER | HIS | GLU | GLN | ALA | ALA | ||||
| 9 | ILE | ALA | LEU | LYS | ASN | ALA | GLY | GLN | THR | VAL | ||||
| 10 | THR | ILE | ILE | ALA | GLN | TYR | LYS | PRO | GLU | GLU | ||||
| 11 | TYR | SER | ARG | PHE | GLU | ALA | ASN | SER | ARG |
Samples:
sample_1: PSD-95 PDZ3, [U-95% 13C; U-95% 15N], 0.562 – 0.562 mM
Neutral_pH_20C: pH: 7.0; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCA | sample_1 | not available | Neutral_pH_20C |
| HN(CO)CA | sample_1 | not available | Neutral_pH_20C |
| HNCO | sample_1 | not available | Neutral_pH_20C |
| HCCH-TOCSY | sample_1 | not available | Neutral_pH_20C |
| TOCSY-HSQC | sample_1 | not available | Neutral_pH_20C |
| NOESY-HSQC | sample_1 | not available | Neutral_pH_20C |
| CBCACONH | sample_1 | not available | Neutral_pH_20C |
| HNHBHA | sample_1 | not available | Neutral_pH_20C |
Software:
NMRPipe v2.2, Rev 2002.365.14.32 - Processing of 2D & 3D data
NMR spectrometers:
- Bruker DMX-Avance 500 MHz
- Varian INOVA 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts