BMRB Entry 6494
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR6494
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Chorismate Mutase PubMed: 15865424
Deposition date: 2005-02-08 Original release date: 2011-08-11
Authors: Eletsky, Alexander; Kienhofer, Alexander; Hilvert, Donald; Pervushin, Konstantin
Citation: Eletsky, Alexander; Kienhofer, Alexander; Hilvert, Donald; Pervushin, Konstantin. "Investigation of Ligand Binding and Protein Dynamics in Bacillus subtilis Chorismate Mutase by Transverse Relaxation Optimized Spectroscopy-Nuclear Magnetic Resonance" Biochemistry 44, 6788-6799 (2005).
Assembly members:
BsCM, polymer, 127 residues, Formula weight is not available
Natural source: Common Name: Bacillus subtilis Taxonomy ID: 1423 Superkingdom: Eubacteria Kingdom: not available Genus/species: Bacillus subtilis
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
BsCM: MMIRGIRGATTVERDTEEEI
LQKTKQLLEKIIEENHTKPE
DVVQMLLSATPDLHAVFPAK
AVRELSGWQYVPVTCMQEMD
VTGGLKKCIRVMMTVQTDVP
QDQIRHVYLEKAVVLRPDLS
LTKNTEL
- assigned_chemical_shifts
- heteronucl_NOEs
- heteronucl_T1_relaxation
- heteronucl_T2_relaxation
| Data type | Count |
| 1H chemical shifts | 245 |
| 13C chemical shifts | 354 |
| 15N chemical shifts | 118 |
| T1 relaxation values | 234 |
| T2 relaxation values | 231 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | BsCM polypeptide_A | 1 |
| 2 | BsCM polypeptide_B | 1 |
| 3 | BsCM polypeptide_C | 1 |
Entities:
Entity 1, BsCM polypeptide_A 127 residues - Formula weight is not available
| 1 | MET | MET | ILE | ARG | GLY | ILE | ARG | GLY | ALA | THR | ||||
| 2 | THR | VAL | GLU | ARG | ASP | THR | GLU | GLU | GLU | ILE | ||||
| 3 | LEU | GLN | LYS | THR | LYS | GLN | LEU | LEU | GLU | LYS | ||||
| 4 | ILE | ILE | GLU | GLU | ASN | HIS | THR | LYS | PRO | GLU | ||||
| 5 | ASP | VAL | VAL | GLN | MET | LEU | LEU | SER | ALA | THR | ||||
| 6 | PRO | ASP | LEU | HIS | ALA | VAL | PHE | PRO | ALA | LYS | ||||
| 7 | ALA | VAL | ARG | GLU | LEU | SER | GLY | TRP | GLN | TYR | ||||
| 8 | VAL | PRO | VAL | THR | CYS | MET | GLN | GLU | MET | ASP | ||||
| 9 | VAL | THR | GLY | GLY | LEU | LYS | LYS | CYS | ILE | ARG | ||||
| 10 | VAL | MET | MET | THR | VAL | GLN | THR | ASP | VAL | PRO | ||||
| 11 | GLN | ASP | GLN | ILE | ARG | HIS | VAL | TYR | LEU | GLU | ||||
| 12 | LYS | ALA | VAL | VAL | LEU | ARG | PRO | ASP | LEU | SER | ||||
| 13 | LEU | THR | LYS | ASN | THR | GLU | LEU |
Samples:
sample_1: BsCM, [U-13C; U-15N; U-2H], mM
sample_2: BsCM, [U-13C; U-15N; U-35% 2H], mM
sample_3: BsCM, [U-15N], mM
conditions_1: pH: 7.5; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| TROSY-CT-HNCA | not available | not available | not available |
| TROSY-HNCACB | not available | not available | not available |
| TROSY-HNCA | not available | not available | not available |
| TROSY-CBCACONH | not available | not available | not available |
| NOESY-TROSY | not available | not available | not available |
| TOCSY-TROSY | not available | not available | not available |
| MQ-HACACO | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- . . . MHz
Related Database Links:
| REF | ZP_03600723 ZP_03592031 ZP_03596312 NP_390150 |
| SP | P19080 |
| GB | ABN13175 ABN05305 ABN05308 AAA20861 AAA22249 |
| DBJ | BAI85767 |
| EMBL | CAB14185 |
| PDB | |
| BMRB | 6495 6496 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts