BMRB Entry 6495
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR6495
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments and relaxation data for Chorismate Mutase from Bacillus subtilis in complex with inhibitor PubMed: 15865424
Deposition date: 2005-02-08 Original release date: 2011-08-11
Authors: Eletsky, Alexander; Kienhofer, Alexander; Hilvert, Donald; Pervushin, Konstantin
Citation: Eletsky, Alexander; Kienhofer, Alexander; Hilvert, Donald; Pervushin, Konstantin. "Investigation of Ligand Binding and Protein Dynamics in Bacillus subtilis Chorismate Mutase by Transverse Relaxation Optimized Spectroscopy-Nuclear Magnetic Resonance" Biochemistry 44, 6788-6799 (2005).
Assembly members:
BsCM, polymer, 127 residues, Formula weight is not available
., non-polymer, Formula weight is not available
Natural source: Common Name: Bacillus subtilis Taxonomy ID: 1423 Superkingdom: Eubacteria Kingdom: not available Genus/species: Bacillus subtilis
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
BsCM: MMIRGIRGATTVERDTEEEI
LQKTKQLLEKIIEENHTKPE
DVVQMLLSATPDLHAVFPAK
AVRELSGWQYVPVTCMQEMD
VTGGLKKCIRVMMTVQTDVP
QDQIRHVYLEKAVVLRPDLS
LTKNTEL
- assigned_chemical_shifts
- heteronucl_NOEs
- heteronucl_T1_relaxation
- heteronucl_T2_relaxation
Data type | Count |
1H chemical shifts | 221 |
13C chemical shifts | 243 |
15N chemical shifts | 119 |
T1 relaxation values | 232 |
T2 relaxation values | 230 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | BsCM polypeptide_A | 1 |
2 | BsCM polypeptide_B | 1 |
3 | BsCM polypeptide_C | 1 |
4 | TSA_A | 2 |
5 | TSA_B | 2 |
6 | TSA_C | 2 |
Entities:
Entity 1, BsCM polypeptide_A 127 residues - Formula weight is not available
1 | MET | MET | ILE | ARG | GLY | ILE | ARG | GLY | ALA | THR | ||||
2 | THR | VAL | GLU | ARG | ASP | THR | GLU | GLU | GLU | ILE | ||||
3 | LEU | GLN | LYS | THR | LYS | GLN | LEU | LEU | GLU | LYS | ||||
4 | ILE | ILE | GLU | GLU | ASN | HIS | THR | LYS | PRO | GLU | ||||
5 | ASP | VAL | VAL | GLN | MET | LEU | LEU | SER | ALA | THR | ||||
6 | PRO | ASP | LEU | HIS | ALA | VAL | PHE | PRO | ALA | LYS | ||||
7 | ALA | VAL | ARG | GLU | LEU | SER | GLY | TRP | GLN | TYR | ||||
8 | VAL | PRO | VAL | THR | CYS | MET | GLN | GLU | MET | ASP | ||||
9 | VAL | THR | GLY | GLY | LEU | LYS | LYS | CYS | ILE | ARG | ||||
10 | VAL | MET | MET | THR | VAL | GLN | THR | ASP | VAL | PRO | ||||
11 | GLN | ASP | GLN | ILE | ARG | HIS | VAL | TYR | LEU | GLU | ||||
12 | LYS | ALA | VAL | VAL | LEU | ARG | PRO | ASP | LEU | SER | ||||
13 | LEU | THR | LYS | ASN | THR | GLU | LEU |
Entity 2, TSA_A 1 residues - . - Formula weight is not available
1 | TSA |
Samples:
15N_BsCM_TSA: BsCM, [U-95% 15N], mM; . mM
DL-BsCM: BsCM, [U-50% 2H; U-95% 13C; U-95% 15N], mM; . mM
15N_BsCM: BsCM, [U-15N], mM
conditions_1: pH: 7.5; temperature: 293 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
TROSY-HNCA | not available | not available | not available |
NOESY-TROSY | not available | not available | not available |
TOCSY-TROSY | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- . . . MHz
Related Database Links:
REF | ZP_03600723 ZP_03592031 ZP_03596312 NP_390150 |
SP | P19080 |
GB | ABN13175 ABN05305 ABN05308 AAA20861 AAA22249 |
DBJ | BAI85767 |
EMBL | CAB14185 |
PDB | |
BMRB | 6494 6496 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts