BMRB Entry 6584
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6584
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Title: Backbone assignment of the E2 ubiquitin conjugating enzyme UbcH5alpha PubMed: 16211490
Deposition date: 2005-04-11 Original release date: 2005-10-25
Authors: Saxena, Krishna; Jacobs, Doris; Vogtherr, Martin; Grimme, Susanne; Elshorst, Bettina; Fiebig, Klaus; Schwalbe, Harald
Citation: Saxena, Krishna; Jacobs, Doris; Vogtherr, Martin; Grimme, Susanne; Elshorst, Bettina; Pescatore, Barbara; Betz, Marco; Schieborr, Ulrich; Langer, Thomas; Schwalbe, Harald; Fiebig, Klaus. "Backbone NMR Assignment of the Human E2 Ubiquitin Conjugating Enzyme UbcH5alpha (F72K,F82S) Double Mutant" J. Biomol. NMR 32, 338-338 (2005).
Assembly members:
Ubiquitin ligating enzyme, polymer, 168 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Ubiquitin ligating enzyme: MGHHHHHHHHHHSSGHIEGR
HMALKRIQKELSDLQRDPPA
HCSAGPVGDDLFHWQATIMG
PPDSAYQGGVFKLTVHFPTD
YPSKPPKIAFTTKIYHPNIN
SNGSICLDILRSQWSPALTV
SKVLLSICSLLCDPNPDDPL
VPDIAQIYKSDKEKYNRHAR
EWTQKYAM
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 268 |
| 15N chemical shifts | 140 |
| 1H chemical shifts | 138 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | UBCH5alpha | 1 |
Entities:
Entity 1, UBCH5alpha 168 residues - Formula weight is not available
| 1 | MET | GLY | HIS | HIS | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | HIS | HIS | SER | SER | GLY | HIS | ILE | GLU | GLY | ARG | ||||
| 3 | HIS | MET | ALA | LEU | LYS | ARG | ILE | GLN | LYS | GLU | ||||
| 4 | LEU | SER | ASP | LEU | GLN | ARG | ASP | PRO | PRO | ALA | ||||
| 5 | HIS | CYS | SER | ALA | GLY | PRO | VAL | GLY | ASP | ASP | ||||
| 6 | LEU | PHE | HIS | TRP | GLN | ALA | THR | ILE | MET | GLY | ||||
| 7 | PRO | PRO | ASP | SER | ALA | TYR | GLN | GLY | GLY | VAL | ||||
| 8 | PHE | LYS | LEU | THR | VAL | HIS | PHE | PRO | THR | ASP | ||||
| 9 | TYR | PRO | SER | LYS | PRO | PRO | LYS | ILE | ALA | PHE | ||||
| 10 | THR | THR | LYS | ILE | TYR | HIS | PRO | ASN | ILE | ASN | ||||
| 11 | SER | ASN | GLY | SER | ILE | CYS | LEU | ASP | ILE | LEU | ||||
| 12 | ARG | SER | GLN | TRP | SER | PRO | ALA | LEU | THR | VAL | ||||
| 13 | SER | LYS | VAL | LEU | LEU | SER | ILE | CYS | SER | LEU | ||||
| 14 | LEU | CYS | ASP | PRO | ASN | PRO | ASP | ASP | PRO | LEU | ||||
| 15 | VAL | PRO | ASP | ILE | ALA | GLN | ILE | TYR | LYS | SER | ||||
| 16 | ASP | LYS | GLU | LYS | TYR | ASN | ARG | HIS | ALA | ARG | ||||
| 17 | GLU | TRP | THR | GLN | LYS | TYR | ALA | MET |
Samples:
sample_1: Ubiquitin ligating enzyme, [U-13C; U-15N; U-60% 2H], 1.7 mM; sodium sulphate 200 mM; sodium phosphate 50 mM; DTT 5 mM
Ex-cond_1: ionic strength: 900 mM; pH: 6.0; temperature: 295 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| HNCO | not available | not available | not available |
| HN(CA)CO | not available | not available | not available |
| HNCACB | not available | not available | not available |
| HN(CO)CACB | not available | not available | not available |
Software:
XEASY - assignment
NMR spectrometers:
- Bruker DRX 800 MHz
- Bruker DRX 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAE25905 BAE28506 BAF84590 BAJ20325 |
| EMBL | CAA55019 CAC82097 CAC82177 |
| GB | AAH05980 AAH15997 AAH19464 AAI11176 AAM81086 |
| REF | NP_001033256 NP_001072142 NP_001102000 NP_001186455 NP_001191809 |
| SP | D3ZDK2 P51668 P61080 Q2TA10 |
| TPG | DAA15007 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts