==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=23-JAN-2010 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER OXIDOREDUCTASE(NAD(A)-CHOH(D)) 12-APR-89 4MDH . COMPND 2 MOLECULE: CYTOPLASMIC MALATE DEHYDROGENASE; . SOURCE 2 ORGANISM_SCIENTIFIC: SUS SCROFA; . AUTHOR J.J.BIRKTOFT,L.J.BANASZAK . 666 2 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 27798.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 453 68.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 55 8.3 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 52 7.8 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 4 0.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 42 6.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 67 10.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 214 32.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 8 1.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 2 4 0 0 0 0 2 2 1 2 1 2 2 2 0 0 0 0 0 0 2 0 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 0 4 2 1 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 4 3 4 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 4 1 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 1 A S 0 0 133 0, 0.0 32,-0.1 0, 0.0 0, 0.0 0.000 360.0 360.0 360.0 63.5 12.9 4.6 33.6 2 2 A E - 0 0 153 1,-0.0 31,-0.0 32,-0.0 30,-0.0 -0.940 360.0-100.3-143.0 124.9 11.9 7.8 31.7 3 3 A P - 0 0 35 0, 0.0 2,-0.3 0, 0.0 32,-0.3 0.425 26.8-155.6 -29.5 154.2 13.4 11.3 31.8 4 4 A I E -a 35 0A 14 30,-1.0 32,-2.1 29,-0.1 2,-0.6 -0.927 20.2-119.6-130.0 151.0 12.1 14.2 33.7 5 5 A R E -a 36 0A 54 74,-0.3 76,-2.2 -2,-0.3 77,-1.5 -0.880 29.4-171.6 -99.7 124.6 12.6 17.8 32.9 6 6 A V E -ab 37 82A 0 30,-1.8 32,-3.2 -2,-0.6 2,-0.4 -0.945 8.3-151.8-116.3 130.3 14.3 19.7 35.6 7 7 A L E -ab 38 83A 0 75,-1.7 77,-2.3 -2,-0.4 2,-0.5 -0.871 9.7-173.1-101.6 134.5 14.6 23.5 35.4 8 8 A V E -ab 39 84A 0 30,-1.8 32,-2.1 -2,-0.4 3,-0.4 -0.928 11.2-160.0-126.9 109.0 17.4 25.3 37.0 9 9 A T E S+a 40 0A 2 75,-2.5 77,-0.5 -2,-0.5 32,-0.2 -0.423 77.4 33.9 -82.4 164.5 17.1 29.1 36.9 10 10 A G S > S+ 0 0 15 30,-0.9 3,-1.1 76,-0.2 6,-0.5 0.945 76.5 164.3 57.2 43.0 20.0 31.4 37.4 11 11 A A T 3 + 0 0 3 29,-2.5 30,-0.2 -3,-0.4 -1,-0.1 0.504 64.6 56.3 -69.3 -13.3 21.9 28.6 35.6 12 12 A A T 3 S+ 0 0 18 28,-0.3 -1,-0.3 4,-0.0 2,-0.2 -0.093 96.6 87.3-107.4 25.6 24.9 30.8 34.9 13 13 A G S <> S- 0 0 32 -3,-1.1 4,-1.3 -4,-0.1 74,-0.0 -0.551 89.9 -85.3-118.4-173.6 25.5 31.8 38.5 14 14 A Q H > S+ 0 0 70 2,-0.2 4,-1.1 1,-0.2 5,-0.1 0.838 119.5 40.3 -62.7 -46.8 27.4 30.7 41.6 15 15 A I H > S+ 0 0 30 1,-0.2 4,-1.7 2,-0.2 3,-0.2 0.906 114.6 54.3 -73.1 -39.6 25.0 28.2 43.2 16 16 A A H > S+ 0 0 0 -6,-0.5 4,-2.0 1,-0.2 -2,-0.2 0.774 104.2 56.9 -54.4 -38.8 24.0 26.7 39.8 17 17 A Y H < S+ 0 0 21 -4,-1.3 4,-0.4 1,-0.2 -1,-0.2 0.843 112.1 40.1 -68.3 -33.4 27.7 26.1 39.1 18 18 A S H < S+ 0 0 24 -4,-1.1 4,-0.4 -3,-0.2 -2,-0.2 0.720 118.7 48.2 -84.3 -22.1 28.1 24.0 42.3 19 19 A L H >X S+ 0 0 1 -4,-1.7 3,-1.1 1,-0.2 4,-1.0 0.900 93.2 70.0 -88.1 -38.5 24.8 22.3 41.9 20 20 A L H 3X S+ 0 0 0 -4,-2.0 4,-2.1 1,-0.3 -1,-0.2 0.730 92.3 58.6 -54.9 -37.8 24.7 21.0 38.2 21 21 A Y H 3> S+ 0 0 54 -4,-0.4 4,-0.8 1,-0.2 -1,-0.3 0.826 108.3 48.9 -62.4 -32.8 27.4 18.3 38.6 22 22 A S H <4 S+ 0 0 13 -3,-1.1 4,-0.2 -4,-0.4 -2,-0.2 0.671 108.5 49.4 -86.1 -12.8 25.2 16.7 41.2 23 23 A I H >< S+ 0 0 2 -4,-1.0 3,-1.5 1,-0.1 5,-0.4 0.955 112.6 49.7 -80.2 -46.1 22.0 16.8 39.3 24 24 A G H 3< S+ 0 0 2 -4,-2.1 39,-0.5 1,-0.3 40,-0.3 0.609 100.4 66.9 -56.2 -25.7 23.9 15.2 36.4 25 25 A N T 3< S- 0 0 47 -4,-0.8 -1,-0.3 38,-0.1 -2,-0.1 0.486 105.6-117.0 -71.8 -24.1 25.4 12.4 38.6 26 26 A G S <> S+ 0 0 2 -3,-1.5 4,-1.8 -4,-0.2 6,-0.2 0.299 86.5 115.8 92.9 -8.7 22.2 10.5 39.4 27 27 A S T 4 S+ 0 0 49 -5,-0.2 -4,-0.1 2,-0.2 4,-0.1 0.373 76.3 40.7 -76.2 5.9 22.8 11.4 43.0 28 28 A V T 4 S+ 0 0 3 -5,-0.4 -1,-0.2 2,-0.3 229,-0.1 0.720 131.5 18.7-113.2 -43.8 19.6 13.6 43.3 29 29 A F T 4 S- 0 0 4 1,-0.3 -2,-0.2 -6,-0.3 2,-0.1 0.350 121.3 -84.7-108.4 -2.0 17.0 11.6 41.3 30 30 A G >< - 0 0 6 -4,-1.8 3,-0.8 1,-0.1 -1,-0.3 -0.293 33.5 -90.1 113.0 160.7 18.6 8.2 41.3 31 31 A K T 3 S+ 0 0 179 1,-0.2 -1,-0.1 -2,-0.1 -4,-0.1 0.747 124.7 47.2 -80.6 -21.6 21.2 6.3 39.3 32 32 A D T 3 S+ 0 0 121 -6,-0.2 -1,-0.2 -3,-0.1 -2,-0.1 0.113 92.5 97.6-107.0 26.7 18.6 4.9 36.9 33 33 A Q < - 0 0 34 -3,-0.8 -4,-0.2 -32,-0.1 2,-0.2 -0.886 59.0-151.5-121.9 109.1 16.8 8.2 36.3 34 34 A P - 0 0 11 0, 0.0 -30,-1.0 0, 0.0 2,-0.3 -0.475 10.9-156.8 -79.7 146.3 17.5 10.4 33.2 35 35 A I E -ac 4 64A 1 28,-1.7 30,-2.0 -32,-0.3 31,-0.7 -0.830 19.4-150.4-118.1 150.9 17.1 14.1 33.1 36 36 A I E -ac 5 66A 32 -32,-2.1 -30,-1.8 -2,-0.3 2,-0.6 -0.997 33.1-146.7-116.8 106.0 16.6 16.6 30.4 37 37 A L E -ac 6 67A 0 29,-3.1 31,-2.5 -2,-0.6 2,-0.5 -0.775 13.6-165.6 -87.6 117.6 18.3 19.5 32.0 38 38 A V E -ac 7 68A 0 -32,-3.2 -30,-1.8 -2,-0.6 2,-0.4 -0.877 5.4-162.8 -99.8 136.3 16.8 22.8 31.2 39 39 A L E -ac 8 69A 2 29,-4.3 31,-2.7 -2,-0.5 2,-0.4 -0.968 4.1-167.4-121.5 135.2 18.8 25.8 32.1 40 40 A L E +ac 9 70A 13 -32,-2.1 -29,-2.5 -2,-0.4 -30,-0.9 -0.953 20.4 131.8-127.5 139.5 17.6 29.4 32.4 41 41 A D - 0 0 29 29,-1.8 2,-0.3 -2,-0.4 5,-0.1 -0.842 54.3 -64.7-155.2-171.4 19.3 32.8 32.6 42 42 A I > - 0 0 104 -2,-0.2 3,-3.2 1,-0.1 4,-0.2 -0.656 51.1-105.4 -92.6 148.6 19.2 36.3 31.2 43 43 A T G > S+ 0 0 90 1,-0.3 3,-1.5 -2,-0.3 4,-0.2 0.826 118.6 56.3 -37.0 -50.9 20.0 37.0 27.6 44 44 A P G 3 S+ 0 0 102 0, 0.0 -1,-0.3 0, 0.0 3,-0.1 0.397 102.1 60.2 -66.1 1.4 23.5 38.4 28.4 45 45 A M G X> S+ 0 0 64 -3,-3.2 4,-1.9 1,-0.1 3,-0.5 0.349 72.4 98.0-112.0 6.7 24.4 35.1 30.1 46 46 A M H <> S+ 0 0 42 -3,-1.5 4,-1.1 -4,-0.2 -1,-0.1 0.693 79.4 54.6 -66.8 -28.7 24.0 32.7 27.3 47 47 A G H 3> S+ 0 0 37 2,-0.2 4,-0.8 -4,-0.2 -1,-0.2 0.764 106.7 53.0 -74.1 -32.4 27.6 32.6 26.3 48 48 A V H X> S+ 0 0 29 -3,-0.5 4,-1.6 2,-0.2 3,-1.0 0.973 109.1 49.2 -63.2 -56.1 28.5 31.6 29.9 49 49 A L H 3X S+ 0 0 1 -4,-1.9 4,-2.3 1,-0.3 5,-0.2 0.746 105.3 57.1 -51.4 -41.2 25.9 28.7 29.7 50 50 A D H 3X S+ 0 0 100 -4,-1.1 4,-1.2 2,-0.2 -1,-0.3 0.872 108.1 50.1 -58.1 -39.7 27.4 27.6 26.4 51 51 A G H < S+ 0 0 83 -4,-1.1 3,-1.1 1,-0.2 -1,-0.2 0.784 109.7 52.2 -70.8 -39.2 30.2 17.1 28.2 58 58 A D H 3< S+ 0 0 5 -4,-1.0 -1,-0.2 1,-0.2 -2,-0.2 0.669 100.9 64.4 -73.8 -15.9 33.7 16.9 29.7 59 59 A C T 3< S- 0 0 1 -4,-0.8 -1,-0.2 -3,-0.1 -2,-0.2 0.256 102.5-129.5 -92.7 6.7 32.1 15.6 32.9 60 60 A A < + 0 0 19 -3,-1.1 -3,-0.1 1,-0.2 -2,-0.1 0.914 41.5 167.6 38.9 78.1 30.8 12.4 31.3 61 61 A L > + 0 0 17 -5,-0.4 3,-1.3 1,-0.1 -1,-0.2 -0.818 13.1 174.9-123.4 85.9 27.2 12.6 32.5 62 62 A P T 3 S+ 0 0 71 0, 0.0 -1,-0.1 0, 0.0 -37,-0.1 0.747 72.3 73.4 -66.0 -20.4 25.1 10.1 30.6 63 63 A L T 3 S+ 0 0 30 -39,-0.5 2,-1.8 1,-0.2 -28,-1.7 0.875 77.1 89.2 -64.4 -25.9 22.0 10.8 32.7 64 64 A L E < +c 35 0A 23 -3,-1.3 -28,-0.2 -40,-0.3 -1,-0.2 -0.442 50.7 175.7 -73.0 81.9 21.7 14.1 30.8 65 65 A K E S- 0 0 123 -30,-2.0 2,-0.3 -2,-1.8 -29,-0.2 0.769 72.8 -2.2 -56.7 -19.8 19.6 13.1 27.8 66 66 A D E -c 36 0A 88 -31,-0.7 -29,-3.1 -3,-0.1 2,-0.4 -0.948 56.0-147.9-173.4 147.1 19.7 16.8 26.9 67 67 A V E -c 37 0A 35 -2,-0.3 2,-0.6 -31,-0.2 -29,-0.2 -0.949 18.2-173.0-131.3 106.6 20.9 20.2 28.0 68 68 A I E -c 38 0A 32 -31,-2.5 -29,-4.3 -2,-0.4 2,-0.5 -0.885 3.2-170.0-102.3 116.5 18.7 23.2 27.0 69 69 A A E +c 39 0A 36 -2,-0.6 2,-0.3 -31,-0.2 -29,-0.2 -0.871 22.7 162.1-103.4 128.7 20.0 26.7 27.7 70 70 A T E -c 40 0A 4 -31,-2.7 -29,-1.8 -2,-0.5 -2,-0.0 -0.988 47.1-167.7-149.0 161.7 17.4 29.4 27.3 71 71 A D S S+ 0 0 64 -2,-0.3 2,-0.4 -31,-0.2 -28,-0.2 0.298 78.1 87.1-116.9 -3.0 16.3 33.0 27.9 72 72 A K > - 0 0 107 1,-0.1 4,-2.0 -30,-0.1 3,-0.2 -0.736 61.4-160.6 -90.9 136.8 12.8 32.3 26.7 73 73 A E H > S+ 0 0 60 -2,-0.4 4,-1.0 2,-0.2 5,-0.2 0.540 88.8 71.0-106.0 7.2 10.4 31.1 29.3 74 74 A E H 4 S+ 0 0 99 2,-0.1 -1,-0.2 3,-0.1 -2,-0.0 0.579 114.2 37.9 -75.7 -9.1 7.9 29.6 26.9 75 75 A I H 4 S+ 0 0 36 -3,-0.2 -2,-0.2 2,-0.1 3,-0.2 0.849 115.8 46.8 -99.3 -55.6 10.9 27.4 26.6 76 76 A A H < S+ 0 0 0 -4,-2.0 -3,-0.2 1,-0.2 -2,-0.1 0.670 118.4 38.7 -64.2 -27.6 12.3 27.1 30.2 77 77 A F S >< S+ 0 0 0 -4,-1.0 3,-1.3 -5,-0.1 -1,-0.2 0.564 84.5 121.3-104.1 -14.3 9.0 26.4 32.0 78 78 A K T 3 S- 0 0 88 1,-0.2 41,-0.3 -3,-0.2 40,-0.1 -0.260 86.6 -13.3 -58.1 127.4 7.4 24.2 29.4 79 79 A D T 3 S+ 0 0 90 39,-2.4 -74,-0.3 1,-0.2 -1,-0.2 0.599 87.0 177.2 53.6 28.6 6.5 20.7 30.7 80 80 A L < - 0 0 0 -3,-1.3 40,-1.0 1,-0.1 42,-0.2 -0.144 30.6-178.7 -67.5 152.5 8.4 20.7 33.9 81 81 A D S S+ 0 0 27 -76,-2.2 43,-1.2 1,-0.5 2,-0.4 0.625 82.7 16.4-110.6 -33.7 8.5 18.0 36.5 82 82 A V E -bd 6 124A 0 -77,-1.5 -75,-1.7 41,-0.2 -1,-0.5 -0.993 66.5-165.7-141.7 137.4 10.7 19.8 38.9 83 83 A A E -bd 7 125A 0 41,-1.9 43,-2.8 -2,-0.4 2,-0.6 -0.992 2.1-165.3-126.7 128.5 11.6 23.5 38.9 84 84 A I E -bd 8 126A 2 -77,-2.3 -75,-2.5 -2,-0.4 2,-1.1 -0.937 7.8-160.5-117.1 108.7 14.5 24.8 40.9 85 85 A L E + d 0 127A 0 41,-3.7 44,-2.2 -2,-0.6 43,-1.8 -0.576 39.1 133.9 -97.9 81.7 14.4 28.6 41.3 86 86 A V + 0 0 18 -2,-1.1 -76,-0.2 -77,-0.5 -1,-0.2 0.815 35.7 116.3 -85.3 -38.7 17.7 30.0 42.2 87 87 A G + 0 0 12 -3,-0.3 2,-0.3 -47,-0.1 -77,-0.1 -0.200 47.6 78.2 -49.2 121.5 17.8 32.9 39.8 88 88 A S S S- 0 0 41 -78,-0.1 16,-0.0 -79,-0.1 -2,-0.0 -0.951 81.8 -46.2 166.8-177.7 17.9 36.3 41.5 89 89 A M - 0 0 72 -2,-0.3 2,-0.2 41,-0.2 41,-0.2 -0.663 51.2-139.1 -78.9 134.4 20.0 38.9 43.3 90 90 A P - 0 0 82 0, 0.0 2,-0.1 0, 0.0 39,-0.0 -0.618 25.4-100.4 -91.6 154.3 22.3 37.7 46.0 91 91 A R - 0 0 82 -2,-0.2 2,-0.3 10,-0.0 149,-0.1 -0.413 40.8-171.2 -69.7 140.8 22.7 39.5 49.4 92 92 A R - 0 0 178 2,-0.3 4,-0.1 -2,-0.1 143,-0.1 -0.952 41.9 -66.9-133.3 157.8 25.9 41.6 49.6 93 93 A D S S+ 0 0 64 -2,-0.3 -1,-0.0 141,-0.2 138,-0.0 -0.176 120.1 66.9 -57.0 144.5 27.4 43.3 52.6 94 94 A G S S+ 0 0 36 -3,-0.0 -2,-0.3 5,-0.0 -1,-0.2 0.643 72.0 110.6 88.0 41.1 25.1 46.0 53.6 95 95 A M S S+ 0 0 4 -4,-0.1 -2,-0.2 2,-0.1 4,-0.1 -0.017 75.0 53.9-115.4 -7.4 23.4 42.9 54.2 96 96 A E S S+ 0 0 91 2,-0.1 -3,-0.1 -4,-0.1 3,-0.0 -0.504 109.1 44.8-129.6 49.8 24.1 44.2 57.6 97 97 A R S S- 0 0 222 -3,-0.1 2,-0.3 -4,-0.0 -2,-0.1 0.140 128.4 -16.4-149.4 -62.0 22.5 47.5 57.1 98 98 A K - 0 0 167 -4,-0.2 2,-0.4 0, 0.0 -2,-0.1 -0.931 47.3-164.1-163.4 135.4 19.2 46.8 55.3 99 99 A D + 0 0 76 -2,-0.3 -4,-0.1 -4,-0.1 -8,-0.0 -0.647 39.6 134.2-122.6 62.0 17.9 43.7 53.5 100 100 A L + 0 0 103 -2,-0.4 2,-1.5 2,-0.0 -1,-0.1 -0.288 21.5 169.3-113.2 48.0 14.9 44.8 51.5 101 101 A L + 0 0 34 1,-0.2 -2,-0.1 2,-0.1 -10,-0.0 -0.535 16.9 148.2 -60.4 87.5 16.2 42.9 48.5 102 102 A K > + 0 0 86 -2,-1.5 4,-0.5 2,-0.1 -1,-0.2 -0.049 52.0 92.4-108.1 -7.2 12.6 43.7 46.9 103 103 A A T 4 S+ 0 0 62 1,-0.2 3,-0.2 2,-0.1 4,-0.1 0.851 86.7 47.5 -55.1 -36.5 14.7 43.7 43.8 104 104 A N T >> S+ 0 0 2 1,-0.2 3,-1.4 2,-0.1 4,-0.9 0.641 85.6 80.2 -83.7 -37.0 13.7 40.0 43.8 105 105 A V H >> S+ 0 0 5 1,-0.3 4,-1.9 2,-0.2 3,-0.8 0.899 87.4 58.9 -56.3 -36.9 10.0 39.8 44.3 106 106 A K H 3X S+ 0 0 104 -4,-0.5 4,-2.0 1,-0.2 -1,-0.3 0.793 99.9 59.1 -60.2 -26.9 9.0 40.6 40.7 107 107 A I H <> S+ 0 0 62 -3,-1.4 4,-1.8 2,-0.2 -1,-0.2 0.806 107.5 45.0 -74.9 -31.0 10.9 37.6 39.4 108 108 A F H X S+ 0 0 73 -4,-1.9 4,-1.4 2,-0.2 3,-0.7 0.967 115.6 43.5 -52.3 -54.6 5.6 36.6 40.7 110 110 A C H 3X S+ 0 0 78 -4,-2.0 4,-1.6 1,-0.3 -2,-0.2 0.861 112.5 52.9 -62.6 -40.0 6.4 36.5 36.9 111 111 A Q H 3X S+ 0 0 20 -4,-1.8 4,-1.6 1,-0.2 -1,-0.3 0.745 105.3 55.5 -68.8 -25.3 7.9 33.0 37.2 112 112 A G H - 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