==== Secondary Structure Definition by the program DSSP, updated CMBI version by ElmK / April 1,2000 ==== DATE=10-MAR-2010 . REFERENCE W. KABSCH AND C.SANDER, BIOPOLYMERS 22 (1983) 2577-2637 . HEADER HYDROLASE 15-DEC-08 2W5V . COMPND 2 MOLECULE: ALKALINE PHOSPHATASE; . SOURCE 2 ORGANISM_SCIENTIFIC: ANTARCTIC BACTERIUM TAB5; . AUTHOR D.KOUTSIOULIS,A.LYSKOWSKI,S.MAKI,E.GUTHRIE,G.FELLER, . 692 2 0 0 0 TOTAL NUMBER OF RESIDUES, NUMBER OF CHAINS, NUMBER OF SS-BRIDGES(TOTAL,INTRACHAIN,INTERCHAIN) . 23888.0 ACCESSIBLE SURFACE OF PROTEIN (ANGSTROM**2) . 485 70.1 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(J) , SAME NUMBER PER 100 RESIDUES . 84 12.1 TOTAL NUMBER OF HYDROGEN BONDS IN PARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 56 8.1 TOTAL NUMBER OF HYDROGEN BONDS IN ANTIPARALLEL BRIDGES, SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-5), SAME NUMBER PER 100 RESIDUES . 4 0.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-4), SAME NUMBER PER 100 RESIDUES . 2 0.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-3), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-2), SAME NUMBER PER 100 RESIDUES . 2 0.3 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I-1), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+0), SAME NUMBER PER 100 RESIDUES . 0 0.0 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+1), SAME NUMBER PER 100 RESIDUES . 40 5.8 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+2), SAME NUMBER PER 100 RESIDUES . 91 13.2 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+3), SAME NUMBER PER 100 RESIDUES . 163 23.6 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+4), SAME NUMBER PER 100 RESIDUES . 10 1.4 TOTAL NUMBER OF HYDROGEN BONDS OF TYPE O(I)-->H-N(I+5), SAME NUMBER PER 100 RESIDUES . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 *** HISTOGRAMS OF *** . 0 0 0 5 0 4 3 2 0 5 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 RESIDUES PER ALPHA HELIX . 0 0 0 8 0 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARALLEL BRIDGES PER LADDER . 8 2 2 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ANTIPARALLEL BRIDGES PER LADDER . 2 2 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LADDERS PER SHEET . # RESIDUE AA STRUCTURE BP1 BP2 ACC N-H-->O O-->H-N N-H-->O O-->H-N TCO KAPPA ALPHA PHI PSI X-CA Y-CA Z-CA 1 30 A Q 0 0 237 0, 0.0 2,-0.1 0, 0.0 320,-0.0 0.000 360.0 360.0 360.0 93.4 -3.9 39.2 -17.1 2 31 A L - 0 0 56 1,-0.2 2,-0.1 319,-0.1 0, 0.0 -0.261 360.0 -87.4 -80.3 177.7 -5.0 38.5 -13.5 3 32 A K - 0 0 135 316,-0.1 -1,-0.2 -2,-0.1 261,-0.0 -0.349 34.6-115.0 -85.4 171.3 -2.9 39.5 -10.5 4 33 A T - 0 0 49 -2,-0.1 2,-0.2 338,-0.1 338,-0.2 -0.927 24.4-145.3-111.5 112.9 -0.1 37.5 -8.9 5 34 A P - 0 0 2 0, 0.0 260,-0.2 0, 0.0 259,-0.1 -0.557 6.0-157.1 -76.7 141.7 -0.7 36.4 -5.3 6 35 A K S S+ 0 0 69 258,-1.7 2,-0.3 -2,-0.2 259,-0.2 0.873 79.4 32.9 -75.1 -42.8 2.3 36.2 -3.0 7 36 A N E -a 265 0A 5 257,-2.2 259,-2.7 212,-0.2 2,-0.4 -0.817 66.7-156.3-118.8 154.0 0.7 33.7 -0.6 8 37 A V E -ab 266 221A 0 212,-2.3 214,-2.0 -2,-0.3 2,-0.5 -0.997 5.0-171.1-133.8 133.4 -1.8 30.8 -1.1 9 38 A I E -ab 267 222A 0 257,-2.8 259,-3.0 -2,-0.4 2,-0.6 -0.988 7.7-168.8-120.2 118.9 -4.2 29.2 1.4 10 39 A L E -ab 268 223A 1 212,-3.3 214,-2.0 -2,-0.5 2,-0.5 -0.939 2.6-167.8-110.6 114.7 -5.9 26.1 0.3 11 40 A L E -ab 269 224A 0 257,-3.0 259,-2.3 -2,-0.6 2,-0.5 -0.904 4.1-164.9-106.3 125.0 -8.7 24.9 2.5 12 41 A I E -ab 270 225A 0 212,-2.4 214,-2.4 -2,-0.5 2,-0.8 -0.939 11.0-162.2-116.4 120.7 -10.0 21.4 1.9 13 42 A S E > - b 0 226A 1 257,-2.3 3,-1.5 -2,-0.5 259,-0.4 -0.871 25.8-136.6 -98.8 105.8 -13.3 20.1 3.2 14 43 A D T 3 S- 0 0 1 212,-1.5 216,-0.6 -2,-0.8 217,-0.1 -0.459 80.9 -0.2 -70.7 119.6 -13.0 16.4 3.0 15 44 A G T 3 S+ 0 0 1 258,-0.5 260,-1.5 -2,-0.4 2,-0.9 0.570 89.9 140.5 86.3 10.9 -16.2 14.8 1.6 16 45 A A < + 0 0 0 -3,-1.5 -1,-0.2 258,-0.2 -3,-0.1 -0.776 23.9 178.3 -95.7 104.5 -18.2 18.0 1.3 17 46 A G > - 0 0 1 -2,-0.9 4,-2.4 258,-0.2 3,-0.3 -0.320 53.6 -85.1 -83.1-178.3 -20.4 18.2 -1.9 18 47 A L H > S+ 0 0 7 294,-0.4 4,-2.7 1,-0.2 641,-0.4 0.839 131.0 57.4 -61.1 -31.7 -22.6 21.2 -2.6 19 48 A S H > S+ 0 0 18 2,-0.2 4,-1.0 1,-0.2 -1,-0.2 0.921 107.3 47.7 -63.3 -42.1 -25.4 19.6 -0.4 20 49 A Q H >> S+ 0 0 0 -3,-0.3 3,-0.9 2,-0.2 4,-0.6 0.945 112.3 49.0 -62.2 -48.8 -22.9 19.5 2.6 21 50 A I H >< S+ 0 0 1 -4,-2.4 3,-1.7 1,-0.2 4,-0.4 0.927 105.6 59.2 -52.1 -46.7 -21.9 23.1 1.9 22 51 A S H >< S+ 0 0 0 -4,-2.7 3,-1.4 1,-0.3 4,-0.4 0.749 88.7 73.2 -58.8 -25.7 -25.6 24.1 1.7 23 52 A S H XX S+ 0 0 0 -4,-1.0 4,-3.0 -3,-0.9 3,-1.7 0.854 87.4 62.4 -61.1 -30.4 -26.1 22.8 5.3 24 53 A T H - 0 0 5 0, 0.0 3,-1.5 0, 0.0 4,-0.1 -0.331 17.2-125.9 -63.9 145.0 -21.8 31.4 6.0 33 62 A N G > S+ 0 0 36 1,-0.3 3,-2.1 2,-0.2 4,-0.3 0.709 103.2 81.7 -66.5 -18.0 -18.4 32.7 5.0 34 63 A Y G > S+ 0 0 0 1,-0.3 3,-1.9 2,-0.2 -1,-0.3 0.846 78.5 69.1 -48.6 -36.2 -19.0 30.9 1.7 35 64 A T G < S+ 0 0 43 -3,-1.5 640,-0.3 1,-0.3 -1,-0.3 0.605 82.3 72.4 -66.2 -9.7 -21.0 34.0 0.7 36 65 A Q G < S+ 0 0 52 -3,-2.1 2,-0.3 -4,-0.1 -1,-0.3 0.702 81.7 93.3 -75.2 -16.9 -17.9 36.1 0.5 37 66 A F < + 0 0 0 -3,-1.9 638,-0.4 -4,-0.3 281,-0.1 -0.568 38.8 167.0 -83.4 137.1 -16.8 34.3 -2.7 38 67 A K + 0 0 116 279,-0.4 636,-1.8 -2,-0.3 2,-0.6 0.738 64.0 72.2-105.8 -44.7 -17.6 35.5 -6.2 39 68 A N E +C 317 0A 22 278,-0.7 278,-2.2 634,-0.2 2,-0.4 -0.628 64.1 171.1 -82.0 118.2 -15.4 33.3 -8.3 40 69 A I E +C 316 0A 1 -2,-0.6 287,-0.7 347,-0.4 347,-0.4 -0.977 7.5 169.5-131.1 136.8 -16.7 29.8 -8.4 41 70 A G E -Cd 315 327A 0 274,-2.8 274,-2.7 -2,-0.4 2,-0.4 -0.736 23.8-127.7-130.9-177.1 -15.7 26.8 -10.5 42 71 A L E -Cd 314 328A 1 285,-2.4 287,-2.3 272,-0.3 2,-0.4 -1.000 15.6-155.4-137.9 138.8 -16.4 23.1 -10.5 43 72 A I E -Cd 313 329A 0 270,-2.6 270,-2.1 -2,-0.4 2,-0.5 -0.911 14.1-136.7-116.4 141.9 -14.0 20.1 -10.6 44 73 A K E - d 0 330A 43 285,-2.0 287,-2.7 -2,-0.4 288,-0.2 -0.823 19.7-165.1 -89.3 132.3 -14.4 16.5 -11.8 45 74 A T + 0 0 0 -2,-0.5 -1,-0.1 265,-0.3 16,-0.1 0.399 35.1 131.8-110.0 1.0 -12.9 14.2 -9.2 46 75 A S - 0 0 10 264,-0.2 5,-0.2 7,-0.1 285,-0.1 -0.159 49.8-130.4 -56.0 149.1 -12.5 10.8 -10.8 47 76 A S - 0 0 14 3,-2.4 22,-0.2 20,-0.1 20,-0.1 -0.156 32.5 -90.3 -87.9-171.1 -9.1 9.1 -10.3 48 77 A S S S+ 0 0 48 20,-1.9 21,-0.2 1,-0.2 3,-0.1 0.712 125.3 17.5 -72.7 -19.8 -6.9 7.6 -13.0 49 78 A R S S+ 0 0 139 19,-0.4 2,-0.4 1,-0.2 -1,-0.2 0.488 119.7 59.8-127.8 -11.4 -8.6 4.2 -12.5 50 79 A E - 0 0 36 1,-0.1 -3,-2.4 4,-0.0 594,-0.2 -0.983 56.8-149.7-134.4 132.7 -11.8 4.8 -10.7 51 80 A D S S+ 0 0 59 593,-1.6 2,-0.6 -2,-0.4 593,-0.2 0.879 98.3 53.6 -61.0 -39.2 -15.0 6.8 -11.4 52 81 A V S S- 0 0 0 591,-2.7 -1,-0.2 -6,-0.1 258,-0.1 -0.887 87.2-148.7-101.8 117.6 -15.3 7.3 -7.6 53 82 A T - 0 0 0 -2,-0.6 2,-0.2 256,-0.2 255,-0.1 -0.355 1.9-143.0 -82.8 159.0 -12.2 8.8 -6.0 54 83 A D > - 0 0 16 -2,-0.1 4,-2.1 -4,-0.1 5,-0.2 -0.623 36.1 -96.8-106.0-179.4 -10.8 8.2 -2.5 55 84 A X H > S+ 0 0 18 -2,-0.2 4,-2.0 1,-0.2 5,-0.2 0.783 117.7 56.4 -68.2 -31.1 -9.1 10.8 -0.4 56 85 A A H > S+ 0 0 0 2,-0.2 4,-2.0 1,-0.2 18,-0.3 0.956 114.2 34.8 -69.7 -53.4 -5.5 9.8 -1.4 57 86 A S H > S+ 0 0 2 2,-0.2 4,-2.0 1,-0.2 -2,-0.2 0.891 117.2 56.5 -68.6 -38.4 -5.9 10.2 -5.2 58 87 A G H X S+ 0 0 0 -4,-2.1 4,-1.0 1,-0.2 -2,-0.2 0.910 113.0 39.3 -57.3 -45.0 -8.2 13.1 -4.7 59 88 A A H X S+ 0 0 0 -4,-2.0 4,-2.9 2,-0.2 5,-0.2 0.832 111.0 57.1 -77.4 -35.4 -5.6 15.1 -2.7 60 89 A T H X>S+ 0 0 0 -4,-2.0 4,-2.6 12,-0.3 6,-0.6 0.833 100.3 60.3 -64.1 -31.6 -2.7 14.0 -4.7 61 90 A A H X5S+ 0 0 1 -4,-2.0 4,-1.2 3,-0.2 274,-0.5 0.927 112.3 39.0 -58.0 -43.9 -4.5 15.5 -7.7 62 91 A F H <5S+ 0 0 3 -4,-1.0 -2,-0.2 2,-0.2 -1,-0.2 0.930 121.9 40.9 -69.3 -47.9 -4.3 18.8 -5.8 63 92 A S H <5S+ 0 0 0 -4,-2.9 22,-2.5 1,-0.2 -3,-0.2 0.791 132.1 20.9 -75.3 -30.2 -0.8 18.5 -4.3 64 93 A C H <5S- 0 0 7 -4,-2.6 -3,-0.2 20,-0.2 -1,-0.2 0.479 95.0-124.0-122.9 -9.2 0.9 17.0 -7.4 65 94 A G S < - 0 0 30 -2,-0.3 3,-2.5 7,-0.2 6,-0.1 -0.443 56.2 -89.5 -80.9 147.4 -4.9 5.5 -8.5 70 99 A N T 3 S+ 0 0 23 1,-0.3 -1,-0.1 -2,-0.1 3,-0.1 -0.281 116.5 25.9 -50.8 134.4 -7.1 5.4 -5.3 71 100 A A T 3 S+ 0 0 16 1,-0.3 48,-1.7 47,-0.1 -1,-0.3 0.128 89.9 131.9 90.6 -16.5 -4.8 5.0 -2.3 72 101 A A < - 0 0 0 -3,-2.5 2,-0.5 46,-0.2 -1,-0.3 -0.424 41.4-158.6 -69.3 142.0 -1.8 6.6 -3.9 73 102 A I B S-J 116 0B 0 43,-2.0 43,-2.2 -2,-0.1 -16,-0.1 -0.939 71.5 -28.4-124.5 106.9 0.0 9.3 -1.9 74 103 A G S S+ 0 0 0 -2,-0.5 8,-1.8 -18,-0.3 2,-0.3 0.766 111.1 110.0 60.8 27.4 2.2 11.7 -3.9 75 104 A V B -K 81 0C 4 6,-0.2 6,-0.2 41,-0.2 -1,-0.2 -0.963 65.4-116.8-130.7 153.9 2.8 9.2 -6.7 76 105 A A > - 0 0 16 4,-2.6 3,-2.3 -2,-0.3 -7,-0.2 -0.109 42.5 -89.5 -78.9 179.3 1.7 8.9 -10.3 77 106 A D T 3 S+ 0 0 58 -10,-0.6 -9,-0.1 1,-0.3 -1,-0.1 0.804 131.5 51.9 -64.4 -27.9 -0.5 6.2 -11.8 78 107 A D T 3 S- 0 0 108 2,-0.1 -1,-0.3 -9,-0.0 -10,-0.0 0.280 121.3-111.2 -88.2 9.6 2.7 4.2 -12.6 79 108 A S S < S+ 0 0 49 -3,-2.3 2,-0.3 1,-0.3 -2,-0.1 0.471 70.1 141.5 77.8 5.0 3.8 4.7 -8.9 80 109 A T - 0 0 71 1,-0.1 -4,-2.6 -5,-0.1 -1,-0.3 -0.611 59.0-106.6 -79.4 136.3 6.7 7.0 -9.6 81 110 A A B -K 75 0C 69 -2,-0.3 2,-0.4 -6,-0.2 -6,-0.2 -0.302 37.1-160.9 -59.2 141.4 7.1 9.8 -7.1 82 111 A V - 0 0 30 -8,-1.8 2,-0.1 -3,-0.0 -17,-0.1 -0.969 25.6 -99.1-128.0 145.6 6.1 13.3 -8.3 83 112 A K - 0 0 84 -2,-0.4 2,-0.2 -19,-0.1 -9,-0.0 -0.377 39.6-143.9 -65.4 132.6 7.0 16.7 -7.0 84 113 A S > - 0 0 1 -2,-0.1 4,-2.8 1,-0.1 -20,-0.2 -0.500 25.8-107.5 -93.5 167.3 4.3 18.2 -4.8 85 114 A I H > S+ 0 0 2 -22,-2.5 4,-3.0 1,-0.2 5,-0.2 0.841 122.0 56.9 -61.8 -32.0 3.3 21.9 -4.5 86 115 A V H > S+ 0 0 0 26,-0.3 4,-2.4 2,-0.2 -1,-0.2 0.915 108.9 44.2 -64.4 -44.5 5.1 21.8 -1.1 87 116 A E H > S+ 0 0 42 2,-0.2 4,-2.0 1,-0.2 -2,-0.2 0.905 115.6 48.1 -65.4 -43.0 8.4 20.7 -2.6 88 117 A I H X S+ 0 0 0 -4,-2.8 4,-1.4 2,-0.2 -2,-0.2 0.927 110.7 51.5 -63.9 -45.0 8.0 23.2 -5.5 89 118 A A H ><>S+ 0 0 0 -4,-3.0 5,-2.4 1,-0.2 3,-0.9 0.952 107.8 52.2 -56.2 -50.6 7.2 26.0 -3.0 90 119 A A H ><5S+ 0 0 32 -4,-2.4 3,-1.9 1,-0.3 -1,-0.2 0.902 106.1 54.2 -52.6 -45.0 10.3 25.2 -0.9 91 120 A L H 3<5S+ 0 0 118 -4,-2.0 -1,-0.3 1,-0.3 -2,-0.2 0.812 108.5 50.4 -58.5 -31.2 12.4 25.4 -4.1 92 121 A N T <<5S- 0 0 48 -4,-1.4 -1,-0.3 -3,-0.9 -2,-0.2 0.130 123.4-104.7 -95.1 17.4 11.0 28.9 -4.7 93 122 A N T < 5S+ 0 0 140 -3,-1.9 126,-0.4 1,-0.2 2,-0.4 0.816 72.4 146.6 66.3 31.2 11.7 30.0 -1.2 94 123 A I < - 0 0 15 -5,-2.4 126,-0.2 -6,-0.1 -1,-0.2 -0.830 47.1-127.6 -99.2 137.3 8.1 29.8 -0.0 95 124 A K E -e 220 0A 57 124,-3.0 126,-2.4 -2,-0.4 2,-0.3 -0.576 29.5-152.0 -80.9 149.7 7.4 28.7 3.6 96 125 A T E +e 221 0A 0 37,-0.3 39,-1.8 124,-0.2 40,-1.3 -0.944 18.3 175.8-132.4 149.9 4.9 25.8 4.0 97 126 A G E -ef 222 136A 0 124,-1.8 126,-2.1 -2,-0.3 2,-0.4 -0.995 11.2-162.5-148.6 148.2 2.3 24.5 6.5 98 127 A V E -ef 223 137A 0 38,-1.8 40,-1.5 -2,-0.3 2,-0.4 -1.000 10.9-178.7-132.8 131.5 -0.3 21.7 6.8 99 128 A V E +ef 224 138A 0 124,-2.6 126,-2.3 -2,-0.4 2,-0.3 -0.989 8.4 164.4-130.0 138.4 -3.1 21.6 9.3 100 129 A A E -e 225 0A 0 38,-2.4 40,-0.2 -2,-0.4 126,-0.1 -0.994 40.8-142.0-150.5 156.7 -5.7 18.9 9.7 101 130 A T E S+ 0 0 0 124,-0.8 90,-1.8 -2,-0.3 2,-0.1 0.471 92.3 66.3 -93.9 -5.8 -8.4 17.7 12.2 102 131 A S E S- 0 0 0 1,-0.2 38,-2.4 88,-0.2 -2,-0.2 -0.317 115.5 -60.2 -89.6-174.1 -7.4 14.1 11.4 103 132 A S E > - f 0 140A 7 36,-0.2 3,-1.9 1,-0.1 38,-0.3 -0.464 50.6-121.4 -58.1 145.3 -4.1 12.5 12.2 104 133 A I T 3 S+ 0 0 0 36,-2.4 -1,-0.1 1,-0.3 37,-0.1 0.626 114.2 60.4 -66.9 -11.8 -1.5 14.5 10.4 105 134 A T T 3 S+ 0 0 0 4,-0.1 -1,-0.3 35,-0.1 20,-0.1 0.455 80.1 117.2 -91.9 -4.5 -0.6 11.2 8.7 106 135 A D S <> S- 0 0 19 -3,-1.9 4,-2.6 1,-0.1 5,-0.2 -0.149 83.9 -94.0 -64.2 165.0 -4.1 10.8 7.1 107 136 A A H > S+ 0 0 1 1,-0.2 4,-1.8 2,-0.2 -1,-0.1 0.808 116.9 51.7 -53.5 -43.2 -4.4 10.8 3.3 108 137 A T H 4 S+ 0 0 0 2,-0.2 4,-0.4 1,-0.2 3,-0.3 0.981 117.8 32.7 -66.6 -59.5 -5.2 14.5 2.8 109 138 A P H >4 S+ 0 0 0 0, 0.0 3,-1.9 0, 0.0 4,-0.4 0.904 115.9 60.9 -61.9 -37.6 -2.3 16.1 4.7 110 139 A A H >X S+ 0 0 0 -4,-2.6 4,-2.6 1,-0.3 3,-1.6 0.858 93.9 62.9 -56.8 -38.2 -0.0 13.2 3.8 111 140 A S T 3< S+ 0 0 1 -4,-1.8 -1,-0.3 -3,-0.3 -3,-0.1 0.700 100.4 55.4 -62.3 -18.1 -0.4 13.9 0.1 112 141 A F T <4 S+ 0 0 0 -3,-1.9 -1,-0.3 -4,-0.4 -26,-0.3 0.561 124.6 15.3 -93.3 -6.9 1.2 17.3 0.6 113 142 A Y T <4 S+ 0 0 6 -3,-1.6 2,-0.3 -4,-0.4 -2,-0.2 0.503 114.2 60.6-141.2 -9.1 4.5 16.1 2.2 114 143 A A < - 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