# 1 "/Bruker/35/exp/stan/nmr/lists/pp/ty_CNOESY_wide"
;ko_CHSQC	2D 1H-13C HSQC experiment (DRX version)
;			T. Kigawa 960605	
;			S. Koshiba 010404
;2D 1H-13C HSQC experiment for 13C/15N-labelled samples 
;dissolved in H2O.
;With 13CO CPD and a 15N 180 pulse
;during t1(13C) evolution period.
;Two spin locking pulses (x,y) immediately after acqu. -> removed
;A 1H 90 degree pulse is added immediately prior to acqu. 
;in order to rotate any residual H2O resonances to the z axis.
;
;F1: 1H		[p1(90deg,pl1)]
;F2: 15N	[p21(90deg,pl4)]
;F3: 13C	[p13(90deg,pl2), PCPD2(90deg,pl12,CDP,GARP)]
;               [p14(90deg,pl7,SHAPE,SQ)]
;With 6 gradient pulses: 
;(400u,+30)*2, (2.5m,+50), (4.0m,-50), (400u,+30)*2

# 1 "/Bruker/35/exp/stan/nmr/lists/pp/Avance.incl" 1
;Avance2.incl
;   for 1
;
;avance-version (03/02/17)





















































































































;$Id: Avance2.incl,v 1.7.2.3 2003/02/25 14:48:48 ber Exp $
# 19 "/Bruker/35/exp/stan/nmr/lists/pp/ty_CNOESY_wide" 2

# 1 "/Bruker/35/exp/stan/nmr/lists/pp/Grad.incl" 1
;Grad2.incl  -  include file for Gradient Spectroscopy
;   for 1
;
;avance-version (02/05/31)



















define list<gradient> EA=<EA>



;$Id: Grad2.incl,v 1.7 2002/06/12 09:04:22 ber Exp $
# 20 "/Bruker/35/exp/stan/nmr/lists/pp/ty_CNOESY_wide" 2

# 1 "/Bruker/35/exp/stan/nmr/lists/pp/De.incl" 1
;De.incl
;
;avance-version (02/05/31)


define delay rde1
define delay rde2
define delay rderx
define delay rdeadc
define delay rdepa


"rde1=de-de1"
"rde2=de-de2"
"rderx=de-derx"
"rdeadc=de-deadc"
"rdepa=de-depa"













;$Id: De.incl,v 1.8 2002/06/12 09:04:22 ber Exp $
# 21 "/Bruker/35/exp/stan/nmr/lists/pp/ty_CNOESY_wide" 2



;"p14=p24/2.0"


"d5=p23/2-p1"
"d15=p23/2-p21"
;"d8=p14-p1"
;"d9=p14-p21"
"d6=(d3-p9-p16-400u-p10-p16-400u)/10-100u-p16"
;

;for gradient pulses
; "p15=400u"
; "p16=2.5m"
; "p17=4.0m"

;for 13C
"d4=1.4m"
"d2=d4-p15-100u"
"d21=d4-p15-5u+p1*2/3.14"
;for AQ
;for t1 evolution
;"d0=in0*3.0-p13*2.0/3.14-p14"
"d0=in0/2-p1*2/3.14"
"d10=in10/2-p13*2.0/3.14-p1"
;"d0=4u"
"l1=td1/2"
"l2=td2/2"


aqseq 312

;pulse sequence.

  30m do:f2
  30m
1 ze
2 75m do:f2
3 30m
4 30m
5 30m
6 20u pl5:f1
  (p7 ph0):f1	; spin lock x
  (p8 ph7):f1	; spin lock y
  20u pl1:f1
  20u pl3:f3
  d1 
  (d5 p1 ph6 d0 d0 p1 ph0 d5):f1 (d0 p23:sp2 ph10):f2 (d0 d15 p21*2 ph10):f3
  50u setnmr3|0 setnmr0|34|32|33
11 50u
  p16:gp0
  50u
  d6
  lo to 11 times 10
  20u pl9:f1
  (p9 ph0):f1
  5u
  p16:gp5
  400u pl11:f1
  (p10 ph0):f1
  5u
  p16:gp6
  400u pl1:f1
;********** INEPT 1H-15N.
  (p1 ph0):f1
;========== 1st gradient pulse.
	50u
	p15:gp1		; 500u +30%
	50u
;========== 
  d2 pl2:f2			; 13C power -> pl2
  (d5 p1*2.0 ph0):f1 (p23:sp2 ph10):f2
;========== 2nd gradient pulse.
	50u
	p15:gp1		; 500u +30%
	50u
;==========
  d2 pl3:f3			; 15N power -> pl3
  (p1 ph1):f1
;========== 3rd gradient pulse (homo-spoil).
	50u
	p16:gp2		; 2.5m	+50%
	50u
;========== t1 (15N) evolution.
  100u pl2:f2
  (p13 ph11):f2
  d10 
  (p1*2.0 ph0):f1
  d10 
  (p13 ph10):f2
;========== 4th gradient pulse (homo-spoil).
	50u
	p17:gp3		; 4.0m -50%
	50u
;==========
  200u
  (p1 ph3):f1
;========== 5th gradient pulse.
	50u
	p15:gp4		; 500u +30%
	50u
;==========
  d2
  (d5 p1*2.0 ph0):f1 (p23:sp2 ph10):f2
;========== 6th gradient pulse (water-gate 2).
	5u
	p15:gp4		; 500u +30%
        d21 setnmr3^0 setnmr0^34^32^33 	; optimal for swh=11160.71,de=6
;=========
;********** 
;  (p1 ph5):f1
;------------------------
;  go=2 ph31 cpd2:f2
  (de1 rde1 adc ph31 syrec) (de2 rde2 ph30:r):f1 (derx rderx setrtp1|1) (deadc rdeadc setrtp1|0|8) (depa rdepa setrtp1|2) (de) ; ph31 for adc, ph30 for f1
;  DE1 DE2 (de) ;(derx rderx setrtp1|1) (deadc rdeadc setrtp1|0|8) (depa rdepa setrtp1|2)
  20u  pl2:f2 cpdngs30:f1
  (p13 ph10 5u p13 ph13):f2
  5u
  vd
  (p23:sp2 ph10):f2
  vd
  vd
  (p23:sp2 ph10):f2
  vd
  20u pl10:f2
  4u cpds2:f2
  aq
  4u do:f2
  4u ivd
  rcyc=2
  60m do:f2 wr #0 if #0 zd
  15m ip6
  lo to 3 times 2
  15m id0
  15m ip31*2
  lo to 4 times l1
  15m rd0
  15m ip11
  lo to 5 times 2
  15m id10
  15m ip31*2
  lo to 6 times l2
;**********
  30m setnmr3^0 setnmr0^34^32^33
  30m
exit

;phases for 1H pulses.
ph0=0
ph1=1 1 3 3
ph3=0
ph5=0 0 0 0 2 2 2 2
ph6=0 0 0 0 2 2 2 2
ph7=1
;phases for X pulses.
ph10=0
ph11=0 2
ph13=0 0 2 2
;phases for y pulse.
ph20=0
;phases for receiver.
ph30=(360) 0
ph31=0 2 2 0 2 0 0 2
;
;pl1 : 1H high power for p1
;pl5 : 1H purge pulse (~25us for 90deg)
;pl3 : 15N high power for p21
;pl2 : 13C high power for p13
;pl7 : 13C power for p14,p24 (sp1)
;pl12: 13C CPD pulse for pcpd2 (~50us at 800MHz)
;pcpd2: 13C CPD pulse (~50us for 90deg at 800MHz)
;
;p1  : 1H 90 deg. (pl1)
;p7  : 1H 8ms (pl5)
;p8  : 1H 5ms (pl5)
;p21  : 15N 90 deg. (pl4)
;p13  : 13C 90 deg. (pl2)
;p14  : 13C 90 deg. (pl7,sp1) for 13CO decoupling
;p24 : 13C 180 deg. (pl7)
;d3 : mixing time 
;gp0 : very very weak grad
;p9 : 2ms (pl9)
;p10 : 2.8ms (pl11)