data_15698 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Solution Structure of the Apo C terminal domain of Lethocerus troponin C isoform F1 ; _BMRB_accession_number 15698 _BMRB_flat_file_name bmr15698.str _Entry_type original _Submission_date 2008-03-29 _Accession_date 2008-03-29 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 'De Nicola' Gian Felice . 2 Kelly Geoff . . 3 Bullard Belinda . . 4 McCormick John . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 371 "13C chemical shifts" 198 "15N chemical shifts" 71 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2010-05-26 update BMRB 'update entity name' 2010-03-19 update BMRB 'completed entry citation' 2010-02-01 original author 'original release' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Solution structure of the Apo C-terminal domain of the Lethocerus F1 troponin C isoform.' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 20104876 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 'De Nicola' 'Gian Felice' . . 2 Martin Stephen . . 3 Bullard Belinda . . 4 Pastore Annalisa . . stop_ _Journal_abbreviation Biochemistry _Journal_name_full Biochemistry _Journal_volume 49 _Journal_issue 8 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 1719 _Page_last 1726 _Year 2010 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'Lethocerus F1 troponin C isoform' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label entity_1 $entity entity_2 $entity entity_3 $entity entity_4 $entity entity_5 $entity entity_6 $entity entity_7 $entity entity_8 $entity entity_9 $entity entity_10 $entity stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_entity _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common 'Lethocerus F1 troponin C isoform' _Molecular_mass 7849.534 _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 70 _Mol_residue_sequence ; MQQELREAFRLYDKEGNGYI STDVMREILAELDETLSSED LDAMIDEIDADGSGTVDFEE FMGVMTGGDE ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 89 MET 2 90 GLN 3 91 GLN 4 92 GLU 5 93 LEU 6 94 ARG 7 95 GLU 8 96 ALA 9 97 PHE 10 98 ARG 11 99 LEU 12 100 TYR 13 101 ASP 14 102 LYS 15 103 GLU 16 104 GLY 17 105 ASN 18 106 GLY 19 107 TYR 20 108 ILE 21 109 SER 22 110 THR 23 111 ASP 24 112 VAL 25 113 MET 26 114 ARG 27 115 GLU 28 116 ILE 29 117 LEU 30 118 ALA 31 119 GLU 32 120 LEU 33 121 ASP 34 122 GLU 35 123 THR 36 124 LEU 37 125 SER 38 126 SER 39 127 GLU 40 128 ASP 41 129 LEU 42 130 ASP 43 131 ALA 44 132 MET 45 133 ILE 46 134 ASP 47 135 GLU 48 136 ILE 49 137 ASP 50 138 ALA 51 139 ASP 52 140 GLY 53 141 SER 54 142 GLY 55 143 THR 56 144 VAL 57 145 ASP 58 146 PHE 59 147 GLU 60 148 GLU 61 149 PHE 62 150 MET 63 151 GLY 64 152 VAL 65 153 MET 66 154 THR 67 155 GLY 68 156 GLY 69 157 ASP 70 158 GLU stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2014-05-12 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 2K2A "Solution Structure Of The Apo C Terminal Domain Of Lethoceru C Isoform F1" 100.00 70 100.00 100.00 9.27e-40 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $entity 'Lethocerus indicus' 212017 Eukaryota Metazoa Lethocerus indicus stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $entity 'recombinant technology' . Escherichia coli . petM11 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity 0.4 mM '[U-99% 15N]' 'sodium chloride' 100 mM 'natural abundance' TRIS 20 mM 'natural abundance' stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity 0.4 mM '[U-99% 15N]' 'sodium chloride' 100 mM 'natural abundance' TRIS 20 mM 'natural abundance' stop_ save_ save_sample_3 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity 0.4 mM '[U-99% 13C; U-99% 15N]' 'sodium chloride' 100 mM 'natural abundance' TRIS 20 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_ARIA _Saveframe_category software _Name ARIA _Version 1.2 loop_ _Vendor _Address _Electronic_address 'Linge, O'Donoghue and Nilges' . . stop_ loop_ _Task refinement 'structure solution' stop_ _Details . save_ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task 'peak picking' processing stop_ _Details . save_ save_XEASY _Saveframe_category software _Name XEASY _Version . loop_ _Vendor _Address _Electronic_address 'Bartels et al.' . . stop_ loop_ _Task 'chemical shift assignment' 'peak picking' stop_ _Details . save_ save_VNMR _Saveframe_category software _Name VNMR _Version . loop_ _Vendor _Address _Electronic_address Varian . . stop_ loop_ _Task collection stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model INOVA _Field_strength 800 _Details . save_ save_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model INOVA _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_2D_1H-13C_HSQC_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HSQC' _Sample_label $sample_3 save_ save_3D_1H-15N_NOESY_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-15N NOESY' _Sample_label $sample_1 save_ save_3D_1H-15N_NOESY_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-15N NOESY' _Sample_label $sample_2 save_ save_2D_1H-15N_HSQC_5 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_2 save_ save_3D_HCCH-TOCSY_6 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HCCH-TOCSY' _Sample_label $sample_3 save_ save_3D_1H-13C_NOESY_7 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-13C NOESY' _Sample_label $sample_3 save_ save_3D_CBCA(CO)NH_8 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $sample_3 save_ save_3D_HN(CO)CA_9 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(CO)CA' _Sample_label $sample_3 save_ save_3D_HNCACB_10 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_3 save_ save_3D_1H-15N_TOCSY_11 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-15N TOCSY' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.1 . M pH 6.8 . pH pressure 1 . atm temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.00 . indirect . . . 0.251449530 DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 DSS N 15 'methyl protons' ppm 0.00 . indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Software_label $XEASY stop_ loop_ _Experiment_label '2D 1H-15N HSQC' '3D 1H-15N NOESY' '3D HCCH-TOCSY' '3D 1H-15N TOCSY' stop_ loop_ _Sample_label $sample_1 $sample_3 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name entity_1 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 89 1 MET H H 8.467 0.000 . 2 89 1 MET N N 120.250 0.000 . 3 90 2 GLN H H 8.276 0.000 . 4 90 2 GLN HE21 H 6.930 0.000 . 5 90 2 GLN HE22 H 7.486 0.000 . 6 90 2 GLN N N 118.747 0.000 . 7 90 2 GLN NE2 N 111.405 0.000 . 8 91 3 GLN H H 8.199 0.000 . 9 91 3 GLN HE21 H 6.862 0.000 . 10 91 3 GLN HE22 H 7.703 0.000 . 11 91 3 GLN N N 120.090 0.000 . 12 91 3 GLN NE2 N 112.217 0.000 . 13 92 4 GLU H H 8.454 0.000 . 14 92 4 GLU N N 120.250 0.000 . 15 93 5 LEU H H 8.410 0.000 . 16 93 5 LEU HA H 4.255 0.012 . 17 93 5 LEU HB2 H 2.054 0.009 . 18 93 5 LEU HB3 H 1.636 0.009 . 19 93 5 LEU HD1 H 0.828 0.005 . 20 93 5 LEU HD2 H 0.865 0.005 . 21 93 5 LEU HG H 1.853 0.009 . 22 93 5 LEU CA C 58.103 0.000 . 23 93 5 LEU CB C 42.309 0.000 . 24 93 5 LEU CD1 C 26.468 0.000 . 25 93 5 LEU CD2 C 24.125 0.000 . 26 93 5 LEU CG C 27.471 0.000 . 27 93 5 LEU N N 121.261 0.000 . 28 94 6 ARG H H 8.345 0.000 . 29 94 6 ARG HA H 4.061 0.006 . 30 94 6 ARG HB2 H 1.983 0.001 . 31 94 6 ARG HB3 H 1.931 0.002 . 32 94 6 ARG HD2 H 3.075 0.005 . 33 94 6 ARG HD3 H 3.075 0.005 . 34 94 6 ARG HE H 7.190 0.000 . 35 94 6 ARG HG2 H 1.692 0.010 . 36 94 6 ARG HG3 H 1.526 0.008 . 37 94 6 ARG CA C 60.241 0.000 . 38 94 6 ARG CB C 30.080 0.000 . 39 94 6 ARG CD C 43.450 0.000 . 40 94 6 ARG CG C 28.449 0.000 . 41 94 6 ARG N N 119.790 0.000 . 42 95 7 GLU H H 8.010 0.000 . 43 95 7 GLU HA H 4.122 0.003 . 44 95 7 GLU HB2 H 2.127 0.003 . 45 95 7 GLU HB3 H 2.127 0.003 . 46 95 7 GLU HG2 H 2.361 0.000 . 47 95 7 GLU HG3 H 2.361 0.000 . 48 95 7 GLU CA C 59.861 0.000 . 49 95 7 GLU CB C 28.812 0.000 . 50 95 7 GLU N N 118.474 0.000 . 51 96 8 ALA H H 8.052 0.000 . 52 96 8 ALA HA H 4.199 0.008 . 53 96 8 ALA HB H 1.670 0.004 . 54 96 8 ALA CA C 55.174 0.000 . 55 96 8 ALA CB C 18.502 0.000 . 56 96 8 ALA N N 122.505 0.000 . 57 97 9 PHE H H 8.432 0.000 . 58 97 9 PHE HA H 3.803 0.003 . 59 97 9 PHE HB2 H 2.999 0.009 . 60 97 9 PHE HB3 H 2.913 0.008 . 61 97 9 PHE HD1 H 6.519 0.009 . 62 97 9 PHE HD2 H 6.519 0.009 . 63 97 9 PHE HE1 H 7.170 0.001 . 64 97 9 PHE HE2 H 7.170 0.001 . 65 97 9 PHE HZ H 7.051 0.003 . 66 97 9 PHE CA C 61.732 0.000 . 67 97 9 PHE CB C 39.537 0.000 . 68 97 9 PHE CD1 C 132.156 0.000 . 69 97 9 PHE CE1 C 131.339 0.000 . 70 97 9 PHE CZ C 129.229 0.000 . 71 97 9 PHE N N 117.510 0.000 . 72 98 10 ARG H H 7.840 0.000 . 73 98 10 ARG HA H 3.955 0.004 . 74 98 10 ARG HB2 H 1.924 0.007 . 75 98 10 ARG HB3 H 1.924 0.007 . 76 98 10 ARG HD2 H 3.196 0.008 . 77 98 10 ARG HD3 H 3.196 0.008 . 78 98 10 ARG HG2 H 1.832 0.003 . 79 98 10 ARG HG3 H 1.694 0.003 . 80 98 10 ARG CA C 59.109 0.000 . 81 98 10 ARG CB C 30.243 0.000 . 82 98 10 ARG CD C 43.457 0.000 . 83 98 10 ARG CG C 28.123 0.000 . 84 98 10 ARG N N 116.256 0.000 . 85 99 11 LEU H H 7.578 0.000 . 86 99 11 LEU HA H 3.958 0.005 . 87 99 11 LEU HB2 H 1.629 0.008 . 88 99 11 LEU HB3 H 1.369 0.005 . 89 99 11 LEU HD1 H 0.735 0.006 . 90 99 11 LEU HD2 H 0.823 0.002 . 91 99 11 LEU HG H 1.488 0.006 . 92 99 11 LEU CA C 57.310 0.000 . 93 99 11 LEU CB C 41.820 0.000 . 94 99 11 LEU CD1 C 23.883 0.000 . 95 99 11 LEU CD2 C 24.862 0.000 . 96 99 11 LEU CG C 26.981 0.000 . 97 99 11 LEU N N 118.446 0.000 . 98 100 12 TYR H H 7.523 0.000 . 99 100 12 TYR HA H 4.369 0.007 . 100 100 12 TYR HB2 H 3.021 0.005 . 101 100 12 TYR HB3 H 2.556 0.005 . 102 100 12 TYR HD1 H 7.141 0.003 . 103 100 12 TYR HD2 H 7.141 0.003 . 104 100 12 TYR HE1 H 6.803 0.000 . 105 100 12 TYR HE2 H 6.803 0.000 . 106 100 12 TYR CA C 59.861 0.000 . 107 100 12 TYR CB C 39.700 0.000 . 108 100 12 TYR CD1 C 133.773 0.000 . 109 100 12 TYR CE1 C 118.294 0.000 . 110 100 12 TYR N N 116.280 0.000 . 111 101 13 ASP H H 7.945 0.000 . 112 101 13 ASP HA H 4.787 0.005 . 113 101 13 ASP HB2 H 2.557 0.002 . 114 101 13 ASP HB3 H 1.936 0.004 . 115 101 13 ASP CA C 52.245 0.000 . 116 101 13 ASP CB C 39.537 0.000 . 117 101 13 ASP N N 121.756 0.000 . 118 102 14 LYS H H 7.858 0.000 . 119 102 14 LYS HA H 4.033 0.008 . 120 102 14 LYS HB2 H 1.837 0.006 . 121 102 14 LYS HB3 H 1.837 0.006 . 122 102 14 LYS HD2 H 1.697 0.014 . 123 102 14 LYS HD3 H 1.697 0.014 . 124 102 14 LYS HE2 H 3.056 0.012 . 125 102 14 LYS HE3 H 3.056 0.012 . 126 102 14 LYS HG2 H 1.478 0.004 . 127 102 14 LYS HG3 H 1.478 0.004 . 128 102 14 LYS CA C 59.269 0.000 . 129 102 14 LYS CB C 32.688 0.000 . 130 102 14 LYS CD C 28.449 0.000 . 131 102 14 LYS CE C 42.472 0.000 . 132 102 14 LYS CG C 25.188 0.000 . 133 102 14 LYS N N 123.213 0.000 . 134 103 15 GLU H H 8.640 0.000 . 135 103 15 GLU HA H 4.218 0.005 . 136 103 15 GLU HB2 H 1.938 0.011 . 137 103 15 GLU HB3 H 2.121 0.004 . 138 103 15 GLU HG2 H 2.290 0.003 . 139 103 15 GLU HG3 H 2.290 0.003 . 140 103 15 GLU CA C 56.981 0.000 . 141 103 15 GLU CB C 29.753 0.000 . 142 103 15 GLU CG C 37.091 0.000 . 143 103 15 GLU N N 116.301 0.000 . 144 104 16 GLY H H 7.824 0.000 . 145 104 16 GLY HA2 H 3.724 0.003 . 146 104 16 GLY HA3 H 3.855 0.005 . 147 104 16 GLY CA C 47.201 0.000 . 148 104 16 GLY N N 109.341 0.000 . 149 105 17 ASN H H 9.620 0.000 . 150 105 17 ASN HA H 4.562 0.009 . 151 105 17 ASN HB2 H 2.414 0.004 . 152 105 17 ASN HB3 H 2.295 0.003 . 153 105 17 ASN HD21 H 7.013 0.000 . 154 105 17 ASN HD22 H 7.636 0.000 . 155 105 17 ASN CA C 54.588 0.000 . 156 105 17 ASN CB C 39.374 0.000 . 157 105 17 ASN N N 120.357 0.000 . 158 105 17 ASN ND2 N 113.929 0.000 . 159 106 18 GLY H H 10.621 0.000 . 160 106 18 GLY HA2 H 3.650 0.006 . 161 106 18 GLY HA3 H 4.265 0.004 . 162 106 18 GLY CA C 45.733 0.000 . 163 106 18 GLY N N 111.026 0.000 . 164 107 19 TYR H H 7.300 0.000 . 165 107 19 TYR HA H 5.472 0.008 . 166 107 19 TYR HB2 H 3.049 0.007 . 167 107 19 TYR HB3 H 2.857 0.005 . 168 107 19 TYR HD1 H 6.782 0.003 . 169 107 19 TYR HD2 H 6.782 0.003 . 170 107 19 TYR HE1 H 6.800 0.003 . 171 107 19 TYR HE2 H 6.800 0.003 . 172 107 19 TYR CA C 56.346 0.000 . 173 107 19 TYR CB C 40.841 0.000 . 174 107 19 TYR CD1 C 134.108 0.000 . 175 107 19 TYR CE1 C 118.294 0.000 . 176 107 19 TYR N N 115.597 0.000 . 177 108 20 ILE H H 8.866 0.000 . 178 108 20 ILE HA H 4.778 0.000 . 179 108 20 ILE HB H 1.753 0.008 . 180 108 20 ILE HD1 H 0.508 0.008 . 181 108 20 ILE HG12 H 1.342 0.003 . 182 108 20 ILE HG13 H 1.029 0.007 . 183 108 20 ILE HG2 H 0.445 0.012 . 184 108 20 ILE CA C 59.275 0.000 . 185 108 20 ILE CB C 42.635 0.000 . 186 108 20 ILE CD1 C 14.263 0.000 . 187 108 20 ILE CG1 C 25.677 0.000 . 188 108 20 ILE CG2 C 17.524 0.000 . 189 108 20 ILE N N 113.436 0.000 . 190 109 21 SER H H 8.620 0.000 . 191 109 21 SER HA H 5.058 0.003 . 192 109 21 SER HB2 H 4.496 0.013 . 193 109 21 SER HB3 H 4.092 0.007 . 194 109 21 SER CA C 57.756 0.000 . 195 109 21 SER CB C 63.883 0.000 . 196 109 21 SER N N 117.553 0.000 . 197 110 22 THR H H 8.354 0.000 . 198 110 22 THR HA H 3.801 0.004 . 199 110 22 THR HB H 4.245 0.006 . 200 110 22 THR HG2 H 1.101 0.002 . 201 110 22 THR CA C 65.633 0.000 . 202 110 22 THR CB C 67.909 0.000 . 203 110 22 THR CG2 C 24.209 0.000 . 204 110 22 THR N N 114.232 0.000 . 205 111 23 ASP H H 8.177 0.000 . 206 111 23 ASP HA H 4.392 0.005 . 207 111 23 ASP HB2 H 2.702 0.008 . 208 111 23 ASP HB3 H 2.595 0.001 . 209 111 23 ASP CA C 57.779 0.000 . 210 111 23 ASP CB C 40.352 0.000 . 211 111 23 ASP N N 120.992 0.000 . 212 112 24 VAL H H 7.504 0.000 . 213 112 24 VAL HA H 3.744 0.006 . 214 112 24 VAL HB H 2.095 0.004 . 215 112 24 VAL HG1 H 0.710 0.005 . 216 112 24 VAL HG2 H 1.033 0.003 . 217 112 24 VAL CA C 65.719 0.000 . 218 112 24 VAL CB C 31.741 0.000 . 219 112 24 VAL CG1 C 21.922 0.000 . 220 112 24 VAL CG2 C 23.353 0.000 . 221 112 24 VAL N N 122.123 0.000 . 222 113 25 MET H H 7.984 0.000 . 223 113 25 MET HA H 4.069 0.003 . 224 113 25 MET HB2 H 2.258 0.001 . 225 113 25 MET HB3 H 1.818 0.002 . 226 113 25 MET HE H 1.922 0.000 . 227 113 25 MET HG2 H 2.468 0.004 . 228 113 25 MET HG3 H 2.352 0.001 . 229 113 25 MET CA C 58.552 0.000 . 230 113 25 MET CB C 33.504 0.000 . 231 113 25 MET CE C 19.155 0.000 . 232 113 25 MET CG C 32.688 0.000 . 233 113 25 MET N N 119.521 0.000 . 234 114 26 ARG H H 8.190 0.000 . 235 114 26 ARG HA H 3.745 0.006 . 236 114 26 ARG HB2 H 1.640 0.008 . 237 114 26 ARG HB3 H 1.640 0.008 . 238 114 26 ARG HD2 H 3.256 0.005 . 239 114 26 ARG HD3 H 3.256 0.005 . 240 114 26 ARG HE H 7.460 0.000 . 241 114 26 ARG HG2 H 1.935 0.008 . 242 114 26 ARG HG3 H 1.935 0.008 . 243 114 26 ARG CA C 60.747 0.000 . 244 114 26 ARG CB C 29.398 0.000 . 245 114 26 ARG CD C 43.457 0.000 . 246 114 26 ARG CG C 30.895 0.000 . 247 114 26 ARG N N 117.832 0.000 . 248 115 27 GLU H H 7.265 0.000 . 249 115 27 GLU HA H 4.010 0.000 . 250 115 27 GLU HB2 H 2.257 0.000 . 251 115 27 GLU HB3 H 2.257 0.000 . 252 115 27 GLU HG2 H 2.480 0.000 . 253 115 27 GLU HG3 H 2.480 0.000 . 254 115 27 GLU CA C 59.435 0.000 . 255 115 27 GLU N N 117.949 0.000 . 256 116 28 ILE H H 8.074 0.000 . 257 116 28 ILE HA H 3.622 0.009 . 258 116 28 ILE HB H 1.875 0.006 . 259 116 28 ILE HD1 H 0.407 0.003 . 260 116 28 ILE HG12 H 1.500 0.004 . 261 116 28 ILE HG13 H 0.662 0.005 . 262 116 28 ILE HG2 H 0.811 0.006 . 263 116 28 ILE CA C 65.633 0.000 . 264 116 28 ILE CB C 38.771 0.000 . 265 116 28 ILE CD1 C 14.752 0.000 . 266 116 28 ILE CG1 C 28.726 0.000 . 267 116 28 ILE CG2 C 17.361 0.000 . 268 116 28 ILE N N 120.582 0.000 . 269 117 29 LEU H H 8.183 0.000 . 270 117 29 LEU HA H 3.901 0.008 . 271 117 29 LEU HB2 H 1.875 0.009 . 272 117 29 LEU HB3 H 1.265 0.009 . 273 117 29 LEU HD1 H 0.843 0.002 . 274 117 29 LEU HD2 H 0.827 0.005 . 275 117 29 LEU HG H 1.855 0.001 . 276 117 29 LEU CA C 58.103 0.000 . 277 117 29 LEU CB C 42.286 0.000 . 278 117 29 LEU CD1 C 25.883 0.000 . 279 117 29 LEU CD2 C 23.068 0.000 . 280 117 29 LEU CG C 27.308 0.000 . 281 117 29 LEU N N 118.919 0.000 . 282 118 30 ALA H H 7.920 0.000 . 283 118 30 ALA HA H 4.131 0.002 . 284 118 30 ALA HB H 1.452 0.008 . 285 118 30 ALA CA C 54.588 0.000 . 286 118 30 ALA CB C 18.992 0.000 . 287 118 30 ALA N N 119.224 0.000 . 288 119 31 GLU H H 7.553 0.000 . 289 119 31 GLU HA H 4.103 0.001 . 290 119 31 GLU HB2 H 2.145 0.000 . 291 119 31 GLU HB3 H 2.145 0.000 . 292 119 31 GLU HG2 H 2.288 0.000 . 293 119 31 GLU HG3 H 2.421 0.000 . 294 119 31 GLU CA C 58.126 0.000 . 295 119 31 GLU CB C 30.080 0.000 . 296 119 31 GLU N N 116.043 0.000 . 297 120 32 LEU H H 7.774 0.000 . 298 120 32 LEU HA H 4.195 0.011 . 299 120 32 LEU HB2 H 1.625 0.009 . 300 120 32 LEU HB3 H 1.833 0.000 . 301 120 32 LEU HD1 H 0.877 0.006 . 302 120 32 LEU HD2 H 0.844 0.003 . 303 120 32 LEU HG H 1.846 0.002 . 304 120 32 LEU CA C 56.946 0.000 . 305 120 32 LEU CB C 42.961 0.000 . 306 120 32 LEU CD1 C 23.720 0.000 . 307 120 32 LEU CD2 C 25.840 0.000 . 308 120 32 LEU CG C 27.144 0.000 . 309 120 32 LEU N N 119.210 0.000 . 310 121 33 ASP H H 7.796 0.000 . 311 121 33 ASP HA H 4.735 0.003 . 312 121 33 ASP HB2 H 2.934 0.002 . 313 121 33 ASP HB3 H 2.391 0.006 . 314 121 33 ASP CA C 53.409 0.011 . 315 121 33 ASP CB C 41.168 0.000 . 316 121 33 ASP N N 117.407 0.000 . 317 122 34 GLU H H 8.884 0.000 . 318 122 34 GLU HA H 4.463 0.007 . 319 122 34 GLU HB2 H 2.139 0.003 . 320 122 34 GLU HB3 H 2.189 0.006 . 321 122 34 GLU HG2 H 2.256 0.002 . 322 122 34 GLU HG3 H 2.367 0.014 . 323 122 34 GLU CA C 57.967 0.000 . 324 122 34 GLU CB C 29.753 0.000 . 325 122 34 GLU CG C 36.113 0.000 . 326 122 34 GLU N N 123.975 0.000 . 327 123 35 THR H H 8.608 0.000 . 328 123 35 THR HA H 4.347 0.006 . 329 123 35 THR HB H 4.350 0.000 . 330 123 35 THR HG2 H 1.253 0.000 . 331 123 35 THR CA C 62.804 0.000 . 332 123 35 THR CB C 69.406 0.000 . 333 123 35 THR CG2 C 22.090 0.000 . 334 123 35 THR N N 111.220 0.000 . 335 124 36 LEU H H 7.104 0.000 . 336 124 36 LEU HA H 4.461 0.006 . 337 124 36 LEU HB2 H 1.832 0.010 . 338 124 36 LEU HB3 H 1.364 0.002 . 339 124 36 LEU HD1 H 0.820 0.007 . 340 124 36 LEU HD2 H 0.941 0.006 . 341 124 36 LEU HG H 1.657 0.006 . 342 124 36 LEU CA C 55.174 0.000 . 343 124 36 LEU CB C 42.635 0.000 . 344 124 36 LEU CD1 C 23.539 0.000 . 345 124 36 LEU CD2 C 26.822 0.000 . 346 124 36 LEU CG C 26.818 0.000 . 347 124 36 LEU N N 122.774 0.000 . 348 125 37 SER H H 9.468 0.000 . 349 125 37 SER HA H 4.594 0.007 . 350 125 37 SER HB2 H 4.397 0.006 . 351 125 37 SER HB3 H 4.108 0.004 . 352 125 37 SER CA C 56.932 0.000 . 353 125 37 SER CB C 66.119 0.000 . 354 125 37 SER N N 121.762 0.000 . 355 126 38 SER H H 8.970 0.000 . 356 126 38 SER HA H 3.925 0.005 . 357 126 38 SER HB2 H 4.080 0.005 . 358 126 38 SER HB3 H 4.080 0.005 . 359 126 38 SER CA C 63.004 0.000 . 360 126 38 SER CB C 62.364 0.000 . 361 126 38 SER N N 116.391 0.000 . 362 127 39 GLU H H 8.468 0.000 . 363 127 39 GLU HA H 4.125 0.005 . 364 127 39 GLU HB2 H 2.130 0.005 . 365 127 39 GLU HB3 H 2.008 0.012 . 366 127 39 GLU HG2 H 2.325 0.000 . 367 127 39 GLU HG3 H 2.410 0.000 . 368 127 39 GLU CA C 60.082 0.000 . 369 127 39 GLU CB C 29.427 0.000 . 370 127 39 GLU CG C 36.928 0.000 . 371 127 39 GLU N N 119.790 0.000 . 372 128 40 ASP H H 7.981 0.000 . 373 128 40 ASP HA H 4.414 0.007 . 374 128 40 ASP HB2 H 2.986 0.006 . 375 128 40 ASP HB3 H 2.637 0.004 . 376 128 40 ASP CA C 57.473 0.000 . 377 128 40 ASP CB C 41.331 0.000 . 378 128 40 ASP N N 122.463 0.000 . 379 129 41 LEU H H 8.289 0.000 . 380 129 41 LEU HA H 4.019 0.006 . 381 129 41 LEU HB2 H 2.003 0.008 . 382 129 41 LEU HB3 H 1.354 0.003 . 383 129 41 LEU HD1 H 0.905 0.001 . 384 129 41 LEU HD2 H 0.881 0.004 . 385 129 41 LEU HG H 1.658 0.006 . 386 129 41 LEU CA C 58.603 0.000 . 387 129 41 LEU CB C 42.146 0.000 . 388 129 41 LEU CD1 C 25.514 0.000 . 389 129 41 LEU CD2 C 26.166 0.000 . 390 129 41 LEU CG C 27.471 0.000 . 391 129 41 LEU N N 121.708 0.000 . 392 130 42 ASP H H 8.353 0.000 . 393 130 42 ASP HA H 4.225 0.004 . 394 130 42 ASP HB2 H 2.830 0.008 . 395 130 42 ASP HB3 H 2.731 0.005 . 396 130 42 ASP CA C 57.797 0.000 . 397 130 42 ASP CB C 40.352 0.000 . 398 130 42 ASP N N 119.133 0.000 . 399 131 43 ALA H H 7.675 0.000 . 400 131 43 ALA HA H 4.196 0.004 . 401 131 43 ALA HB H 1.543 0.003 . 402 131 43 ALA CA C 55.002 0.000 . 403 131 43 ALA CB C 18.267 0.000 . 404 131 43 ALA N N 121.142 0.000 . 405 132 44 MET H H 8.039 0.000 . 406 132 44 MET HA H 4.153 0.006 . 407 132 44 MET HB2 H 2.217 0.008 . 408 132 44 MET HB3 H 2.035 0.008 . 409 132 44 MET HE H 2.067 0.003 . 410 132 44 MET HG2 H 2.819 0.005 . 411 132 44 MET HG3 H 2.500 0.005 . 412 132 44 MET CA C 59.435 0.000 . 413 132 44 MET CB C 33.830 0.000 . 414 132 44 MET CE C 17.850 0.000 . 415 132 44 MET CG C 33.015 0.000 . 416 132 44 MET N N 118.616 0.000 . 417 133 45 ILE H H 8.322 0.000 . 418 133 45 ILE HA H 3.718 0.004 . 419 133 45 ILE HB H 2.202 0.005 . 420 133 45 ILE HD1 H 0.669 0.005 . 421 133 45 ILE HG12 H 1.689 0.005 . 422 133 45 ILE HG13 H 1.420 0.006 . 423 133 45 ILE HG2 H 0.948 0.007 . 424 133 45 ILE CA C 62.804 0.000 . 425 133 45 ILE CB C 35.787 0.000 . 426 133 45 ILE CD1 C 10.512 0.000 . 427 133 45 ILE CG1 C 28.123 0.000 . 428 133 45 ILE CG2 C 18.267 0.000 . 429 133 45 ILE N N 119.464 0.000 . 430 134 46 ASP H H 7.995 0.000 . 431 134 46 ASP HA H 4.340 0.000 . 432 134 46 ASP HB2 H 2.758 0.004 . 433 134 46 ASP HB3 H 2.633 0.001 . 434 134 46 ASP CA C 57.317 0.000 . 435 134 46 ASP CB C 41.005 0.000 . 436 135 47 GLU H H 7.371 0.000 . 437 135 47 GLU HA H 4.106 0.004 . 438 135 47 GLU HB2 H 2.134 0.004 . 439 135 47 GLU HB3 H 2.134 0.004 . 440 135 47 GLU HG2 H 2.413 0.017 . 441 135 47 GLU HG3 H 2.251 0.010 . 442 135 47 GLU CA C 58.543 0.000 . 443 135 47 GLU CB C 29.916 0.000 . 444 135 47 GLU CG C 36.602 0.000 . 445 135 47 GLU N N 116.443 0.000 . 446 136 48 ILE H H 8.523 0.000 . 447 136 48 ILE HA H 4.000 0.000 . 448 136 48 ILE HB H 1.815 0.005 . 449 136 48 ILE HD1 H 0.828 0.002 . 450 136 48 ILE HG12 H 1.195 0.006 . 451 136 48 ILE HG13 H 1.762 0.021 . 452 136 48 ILE HG2 H 0.938 0.006 . 453 136 48 ILE CA C 63.676 0.000 . 454 136 48 ILE CB C 39.357 0.000 . 455 136 48 ILE CD1 C 14.952 0.000 . 456 136 48 ILE CG1 C 28.775 0.000 . 457 136 48 ILE CG2 C 18.267 0.000 . 458 136 48 ILE N N 121.250 0.000 . 459 137 49 ASP H H 7.840 0.000 . 460 137 49 ASP HA H 5.106 0.006 . 461 137 49 ASP HB2 H 2.151 0.005 . 462 137 49 ASP HB3 H 2.846 0.005 . 463 137 49 ASP CA C 52.581 0.000 . 464 137 49 ASP CB C 40.189 0.000 . 465 137 49 ASP N N 117.820 0.000 . 466 138 50 ALA H H 7.846 0.000 . 467 138 50 ALA HA H 4.181 0.005 . 468 138 50 ALA HB H 1.487 0.009 . 469 138 50 ALA CA C 55.346 0.000 . 470 138 50 ALA CB C 19.318 0.000 . 471 138 50 ALA N N 125.638 0.000 . 472 139 51 ASP H H 8.485 0.000 . 473 139 51 ASP HA H 4.653 0.007 . 474 139 51 ASP HB2 H 2.680 0.002 . 475 139 51 ASP HB3 H 2.847 0.002 . 476 139 51 ASP CB C 40.841 0.000 . 477 139 51 ASP N N 113.885 0.000 . 478 140 52 GLY H H 7.900 0.000 . 479 140 52 GLY HA2 H 3.845 0.001 . 480 140 52 GLY HA3 H 3.845 0.001 . 481 140 52 GLY CA C 47.527 0.000 . 482 140 52 GLY N N 113.060 0.000 . 483 141 53 SER H H 9.150 0.000 . 484 141 53 SER HA H 4.227 0.005 . 485 141 53 SER HB2 H 3.993 0.007 . 486 141 53 SER HB3 H 3.875 0.004 . 487 141 53 SER CA C 60.461 0.000 . 488 141 53 SER CB C 64.645 0.000 . 489 141 53 SER N N 117.771 0.000 . 490 142 54 GLY H H 10.554 0.000 . 491 142 54 GLY HA2 H 3.781 0.003 . 492 142 54 GLY HA3 H 4.210 0.004 . 493 142 54 GLY CA C 46.222 0.000 . 494 142 54 GLY N N 115.432 0.000 . 495 143 55 THR H H 7.940 0.000 . 496 143 55 THR HA H 5.081 0.001 . 497 143 55 THR HB H 3.852 0.002 . 498 143 55 THR HG2 H 0.820 0.003 . 499 143 55 THR CA C 59.861 0.000 . 500 143 55 THR CB C 72.835 0.000 . 501 143 55 THR CG2 C 22.090 0.000 . 502 143 55 THR N N 110.471 0.000 . 503 144 56 VAL H H 9.090 0.000 . 504 144 56 VAL HA H 4.553 0.004 . 505 144 56 VAL HB H 2.052 0.006 . 506 144 56 VAL HG1 H 1.044 0.008 . 507 144 56 VAL HG2 H 0.816 0.003 . 508 144 56 VAL CA C 61.032 0.000 . 509 144 56 VAL CB C 35.256 0.000 . 510 144 56 VAL CG1 C 21.796 0.000 . 511 144 56 VAL CG2 C 21.274 0.000 . 512 144 56 VAL N N 119.565 0.000 . 513 145 57 ASP H H 8.870 0.000 . 514 145 57 ASP HA H 5.088 0.012 . 515 145 57 ASP HB2 H 3.001 0.010 . 516 145 57 ASP HB3 H 2.836 0.007 . 517 145 57 ASP CA C 53.231 0.000 . 518 145 57 ASP CB C 42.146 0.000 . 519 145 57 ASP N N 126.198 0.000 . 520 146 58 PHE H H 8.223 0.000 . 521 146 58 PHE HA H 3.538 0.005 . 522 146 58 PHE HB2 H 2.633 0.004 . 523 146 58 PHE HB3 H 2.502 0.004 . 524 146 58 PHE HD1 H 6.533 0.005 . 525 146 58 PHE HD2 H 6.533 0.005 . 526 146 58 PHE HE1 H 7.000 0.002 . 527 146 58 PHE HE2 H 7.000 0.002 . 528 146 58 PHE HZ H 7.214 0.011 . 529 146 58 PHE CA C 61.718 0.000 . 530 146 58 PHE CB C 39.211 0.000 . 531 146 58 PHE CD1 C 132.156 0.000 . 532 146 58 PHE CE1 C 131.339 0.000 . 533 146 58 PHE CZ C 130.035 0.000 . 534 147 59 GLU H H 8.347 0.000 . 535 147 59 GLU HA H 3.746 0.005 . 536 147 59 GLU HB2 H 2.013 0.008 . 537 147 59 GLU HB3 H 2.074 0.007 . 538 147 59 GLU HG2 H 2.334 0.008 . 539 147 59 GLU HG3 H 2.334 0.008 . 540 147 59 GLU CA C 60.061 0.000 . 541 147 59 GLU CB C 29.101 0.000 . 542 147 59 GLU CG C 37.091 0.000 . 543 147 59 GLU N N 118.710 0.000 . 544 148 60 GLU H H 8.380 0.000 . 545 148 60 GLU HA H 4.037 0.000 . 546 148 60 GLU HB2 H 1.990 0.000 . 547 148 60 GLU HB3 H 2.326 0.000 . 548 148 60 GLU HG2 H 2.501 0.000 . 549 148 60 GLU HG3 H 2.501 0.000 . 550 148 60 GLU CA C 59.920 0.000 . 551 148 60 GLU CB C 30.080 0.000 . 552 148 60 GLU N N 121.774 0.000 . 553 149 61 PHE H H 8.421 0.000 . 554 149 61 PHE HA H 4.083 0.001 . 555 149 61 PHE HB2 H 3.196 0.003 . 556 149 61 PHE HB3 H 3.068 0.004 . 557 149 61 PHE HD1 H 7.078 0.000 . 558 149 61 PHE HD2 H 7.078 0.000 . 559 149 61 PHE HE1 H 7.366 0.005 . 560 149 61 PHE HE2 H 7.366 0.005 . 561 149 61 PHE HZ H 7.300 0.011 . 562 149 61 PHE CA C 61.232 0.000 . 563 149 61 PHE CB C 39.863 0.000 . 564 149 61 PHE CD1 C 132.480 0.000 . 565 149 61 PHE CE1 C 131.826 0.000 . 566 149 61 PHE CZ C 129.545 0.000 . 567 149 61 PHE N N 121.039 0.000 . 568 150 62 MET H H 8.446 0.000 . 569 150 62 MET HA H 3.857 0.001 . 570 150 62 MET HB2 H 1.726 0.017 . 571 150 62 MET HB3 H 1.778 0.017 . 572 150 62 MET HE H 2.099 0.007 . 573 150 62 MET HG2 H 1.960 0.002 . 574 150 62 MET HG3 H 2.121 0.008 . 575 150 62 MET CA C 57.310 0.000 . 576 150 62 MET CB C 31.221 0.000 . 577 150 62 MET CE C 17.361 0.000 . 578 150 62 MET CG C 32.327 0.000 . 579 150 62 MET N N 117.673 0.000 . 580 151 63 GLY H H 7.889 0.000 . 581 151 63 GLY HA2 H 3.774 0.006 . 582 151 63 GLY HA3 H 3.871 0.003 . 583 151 63 GLY CA C 47.038 0.000 . 584 151 63 GLY N N 107.497 0.000 . 585 152 64 VAL H H 7.571 0.000 . 586 152 64 VAL HA H 3.937 0.010 . 587 152 64 VAL HB H 2.037 0.006 . 588 152 64 VAL HG1 H 0.877 0.007 . 589 152 64 VAL HG2 H 0.996 0.002 . 590 152 64 VAL CA C 64.547 0.000 . 591 152 64 VAL CB C 32.362 0.000 . 592 152 64 VAL CG1 C 21.600 0.000 . 593 152 64 VAL CG2 C 22.368 0.000 . 594 152 64 VAL N N 119.340 0.000 . 595 153 65 MET H H 7.773 0.000 . 596 153 65 MET HA H 4.367 0.004 . 597 153 65 MET HB2 H 1.716 0.009 . 598 153 65 MET HB3 H 1.866 0.010 . 599 153 65 MET HE H 1.862 0.000 . 600 153 65 MET HG2 H 2.041 0.004 . 601 153 65 MET HG3 H 2.041 0.004 . 602 153 65 MET CA C 55.874 0.000 . 603 153 65 MET CB C 32.525 0.000 . 604 153 65 MET CE C 17.198 0.000 . 605 153 65 MET CG C 32.036 0.000 . 606 153 65 MET N N 117.775 0.000 . 607 154 66 THR H H 7.789 0.000 . 608 154 66 THR HA H 4.403 0.004 . 609 154 66 THR HB H 4.318 0.005 . 610 154 66 THR HG2 H 1.169 0.002 . 611 154 66 THR CA C 62.204 0.000 . 612 154 66 THR CB C 70.577 0.000 . 613 154 66 THR CG2 C 21.764 0.000 . 614 154 66 THR N N 110.197 0.000 . 615 155 67 GLY H H 7.961 0.000 . 616 155 67 GLY HA2 H 4.017 0.002 . 617 155 67 GLY HA3 H 4.017 0.002 . 618 155 67 GLY CA C 45.896 0.000 . 619 155 67 GLY N N 110.658 0.000 . 620 156 68 GLY H H 8.313 0.000 . 621 156 68 GLY HA2 H 3.990 0.001 . 622 156 68 GLY HA3 H 3.990 0.001 . 623 156 68 GLY CA C 45.407 0.000 . 624 156 68 GLY N N 108.792 0.000 . 625 157 69 ASP H H 8.290 0.000 . 626 157 69 ASP HA H 4.666 0.005 . 627 157 69 ASP HB2 H 2.736 0.004 . 628 157 69 ASP HB3 H 2.583 0.005 . 629 157 69 ASP CA C 54.588 0.000 . 630 157 69 ASP CB C 41.700 0.000 . 631 157 69 ASP N N 120.766 0.000 . 632 158 70 GLU H H 7.988 0.000 . 633 158 70 GLU HA H 4.111 0.000 . 634 158 70 GLU HB2 H 1.955 0.000 . 635 158 70 GLU HB3 H 1.955 0.000 . 636 158 70 GLU HG2 H 2.211 0.000 . 637 158 70 GLU HG3 H 2.211 0.000 . 638 158 70 GLU CA C 58.040 0.000 . 639 158 70 GLU CB C 31.270 0.000 . 640 158 70 GLU N N 125.726 0.000 . stop_ save_