Assignment Validation Software (AVS)
(c) Hunter Moseley and Gaetano Montelione, 2004


FLAG polypeptide(L)
1.A1	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Indeterminable 	N Shifts: Indeterminable




FLAG polypeptide(L)
1.D2	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	HA :: 4.450	
	Ave H Shift Values>> 	H :: 8.33(0.57) 	HA :: 4.61(0.31) 	HB2 :: 2.74(0.29) 	HB3 :: 2.7(0.29)



FLAG polypeptide(L)
1.E3	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 8.214	 	HA :: 4.213	 	HB2 :: 2.164	 	HB3 :: 1.591	 	HG2 :: 1.789	 	HG3 :: 2.103	
	Ave H Shift Values>> 	H :: 8.34(0.61) 	HA :: 4.26(0.43) 	HB2 :: 2.04(0.22) 	HB3 :: 2.04(0.23) 	HG2 :: 2.31(0.21) 	HG3 :: 2.31(0.21)



FLAG polypeptide(L)
1.T4	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 8.208	 	HA :: 4.367	 	HB :: 4.013	 	HG21 :: 0.550	 	HG22 :: 0.550	 	HG23 :: 0.550	
	Ave H Shift Values>> 	H :: 8.27(0.62) 	HA :: 4.48(0.5) 	HB :: 4.17(0.37) 	HG1 :: 4.4(1.92) 	HG2 :: 1.16(0.28) 	HG21 :: 1.16(0.28)
			 	HG22 :: 1.16(0.28) 	HG23 :: 1.16(0.28)



FLAG polypeptide(L)
1.L5	Overall: Suspicious 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Suspicious 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 8.068	 	HA :: 3.094	 	HB2 :: 0.727	 	HB3 :: 0.863	 	HG :: -0.152(S)	 	HD11 :: -0.108	
			 	HD12 :: -0.108	 	HD13 :: -0.108	 	HD21 :: 0.122	 	HD22 :: 0.122	 	HD23 :: 0.122	
	Ave H Shift Values>> 	H :: 8.22(0.65) 	HA :: 4.32(0.47) 	HB2 :: 1.63(0.34) 	HB3 :: 1.57(0.36) 	HG :: 1.54(0.33) 	HD1 :: 0.77(0.27)
			 	HD2 :: 0.76(0.28)



FLAG polypeptide(L)
1.A6	Overall: Anomalous 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Anomalous 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 7.938	 	HA :: 2.662(S)	 	HB1 :: -1.578(A)	 	HB2 :: -1.578(A)	 	HB3 :: -1.578(A)	
	Ave H Shift Values>> 	H :: 8.2(0.6) 	HA :: 4.26(0.42) 	HB :: 1.38(0.25)



FLAG polypeptide(L)
1.E7	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 6.938	 	HA :: 3.273	 	HB2 :: 1.705	 	HB3 :: 1.757	 	HG2 :: 1.942	 	HG3 :: 1.851	
	Ave H Shift Values>> 	H :: 8.34(0.61) 	HA :: 4.26(0.43) 	HB2 :: 2.04(0.22) 	HB3 :: 2.04(0.23) 	HG2 :: 2.31(0.21) 	HG3 :: 2.31(0.21)



FLAG polypeptide(L)
1.F8	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 7.568	 	HA :: 3.752	 	HB2 :: 2.471	 	HB3 :: 2.155	 	HD1 :: 5.739	 	HD2 :: 5.740	
			 	HE1 :: 6.750	 	HE2 :: 6.751	 	HZ :: 7.466	
	Ave H Shift Values>> 	H :: 8.42(0.74) 	HA :: 4.62(0.58) 	HB2 :: 2.99(0.37) 	HB3 :: 2.97(0.38) 	HD1 :: 6.91(0.82) 	HD2 :: 6.89(0.86)
			 	HE1 :: 6.95(0.82) 	HE2 :: 6.94(0.86) 	HZ :: 6.92(0.8)



FLAG polypeptide(L)
1.H9	Overall: Anomalous 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Anomalous 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 6.505	 	HA :: 2.781(S)	 	HB2 :: 1.956	 	HB3 :: 1.861	 	HD1 :: 8.607	 	HD2 :: 1.101(A)	
			 	HE1 :: 1.309(A)	
	Ave H Shift Values>> 	H :: 8.25(0.74) 	HA :: 4.62(0.51) 	HB2 :: 3.12(0.43) 	HB3 :: 3.11(0.45) 	HD1 :: 10.14(3.5) 	HD2 :: 7.08(0.5)
			 	HE1 :: 8.08(0.57) 	HE2 :: 10.43(3.11)



FLAG polypeptide(L)
1.V10	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 8.569	 	HA :: 3.499	 	HB :: 2.272	 	HG11 :: 1.097	 	HG12 :: 1.097	 	HG13 :: 1.097	
			 	HG21 :: 1.573	 	HG22 :: 1.573	 	HG23 :: 1.573	
	Ave H Shift Values>> 	H :: 8.29(0.71) 	HA :: 4.16(0.57) 	HB :: 1.99(0.33) 	HG1 :: 0.84(0.27) 	HG2 :: 0.83(0.29)



FLAG polypeptide(L)
1.E11	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 7.185	 	HA :: 3.843	 	HB2 :: 1.851	 	HB3 :: 1.761	 	HG2 :: 2.120	 	HG3 :: 2.285	
	Ave H Shift Values>> 	H :: 8.34(0.61) 	HA :: 4.26(0.43) 	HB2 :: 2.04(0.22) 	HB3 :: 2.04(0.23) 	HG2 :: 2.31(0.21) 	HG3 :: 2.31(0.21)



FLAG polypeptide(L)
1.M12	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 6.578	 	HA :: 4.240	 	HB2 :: 1.953	 	HB3 :: 1.860	 	HG2 :: 2.196	 	HE1 :: 0.314	
			 	HE2 :: 0.314	 	HE3 :: 0.314	
	Ave H Shift Values>> 	H :: 8.26(0.6) 	HA :: 4.39(0.46) 	HB2 :: 2.03(0.38) 	HB3 :: 2.01(0.36) 	HG2 :: 2.44(0.36) 	HG3 :: 2.41(0.4)
			 	HE :: 1.86(0.47)



FLAG polypeptide(L)
1.G13	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 7.583	 	HA2 :: 4.459	 	HA3 :: 3.916	
	Ave H Shift Values>> 	H :: 8.34(0.74) 	HA2 :: 3.95(0.4) 	HA3 :: 3.93(0.4)



FLAG polypeptide(L)
1.G14	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 8.818	 	HA2 :: 4.646	 	HA3 :: 4.203	
	Ave H Shift Values>> 	H :: 8.34(0.74) 	HA2 :: 3.95(0.4) 	HA3 :: 3.93(0.4)



FLAG polypeptide(L)
1.C15	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 9.211	 	HA :: 5.103	 	HB2 :: 2.641	 	HB3 :: 2.766	
	Ave H Shift Values>> 	H :: 8.42(0.66) 	HA :: 4.73(0.57) 	HB2 :: 2.95(0.43) 	HB3 :: 2.98(0.45) 	HG :: 1.66(0.48)



FLAG polypeptide(L)
1.E16	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 9.687	 	HA :: 4.684	 	HB2 :: 2.026	 	HB3 :: 1.917	 	HG2 :: 2.370	 	HG3 :: 2.108	
	Ave H Shift Values>> 	H :: 8.34(0.61) 	HA :: 4.26(0.43) 	HB2 :: 2.04(0.22) 	HB3 :: 2.04(0.23) 	HG2 :: 2.31(0.21) 	HG3 :: 2.31(0.21)



FLAG polypeptide(L)
1.N17	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 7.564	 	HA :: 4.312	 	HB2 :: 2.663	 	HD21 :: 6.238	 	HD22 :: 5.737	
	Ave H Shift Values>> 	H :: 8.37(0.65) 	HA :: 4.7(0.39) 	HB2 :: 2.8(0.34) 	HB3 :: 2.78(0.34) 	HD21 :: 7.27(0.52) 	HD22 :: 7.2(0.52)



FLAG polypeptide(L)
1.C18	Overall: Suspicious 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Suspicious 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 6.420	 	HA :: 4.663	 	HB2 :: 0.629(S)	 	HB3 :: 2.189	
	Ave H Shift Values>> 	H :: 8.42(0.66) 	HA :: 4.73(0.57) 	HB2 :: 2.95(0.43) 	HB3 :: 2.98(0.45) 	HG :: 1.66(0.48)



FLAG polypeptide(L)
1.H19	Overall: Anomalous 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Anomalous 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 6.637	 	HA :: 3.768	 	HB2 :: 0.573(S)	 	HB3 :: 0.797(S)	 	HD1 :: 10.802	 	HD2 :: 0.139(A)	
			 	HE1 :: 0.867(A)	
	Ave H Shift Values>> 	H :: 8.25(0.74) 	HA :: 4.62(0.51) 	HB2 :: 3.12(0.43) 	HB3 :: 3.11(0.45) 	HD1 :: 10.14(3.5) 	HD2 :: 7.08(0.5)
			 	HE1 :: 8.08(0.57) 	HE2 :: 10.43(3.11)



FLAG polypeptide(L)
1.A20	Overall: Suspicious 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Suspicious 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 7.669	 	HA :: 3.596	 	HB1 :: 0.236(S)	 	HB2 :: 0.236(S)	 	HB3 :: 0.236(S)	
	Ave H Shift Values>> 	H :: 8.2(0.6) 	HA :: 4.26(0.42) 	HB :: 1.38(0.25)



FLAG polypeptide(L)
1.D21	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 9.152	 	HA :: 4.059	 	HB2 :: 2.714	 	HB3 :: 2.368	
	Ave H Shift Values>> 	H :: 8.33(0.57) 	HA :: 4.61(0.31) 	HB2 :: 2.74(0.29) 	HB3 :: 2.7(0.29)



FLAG polypeptide(L)
1.G22	Overall: Suspicious 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Suspicious 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 7.497	 	HA2 :: 3.774	 	HA3 :: 2.380(S)	
	Ave H Shift Values>> 	H :: 8.34(0.74) 	HA2 :: 3.95(0.4) 	HA3 :: 3.93(0.4)



FLAG polypeptide(L)
1.E23	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 6.896	 	HA :: 4.029	 	HB2 :: 1.473	 	HB3 :: 1.359	 	HG2 :: 1.855	 	HG3 :: 1.810	
	Ave H Shift Values>> 	H :: 8.34(0.61) 	HA :: 4.26(0.43) 	HB2 :: 2.04(0.22) 	HB3 :: 2.04(0.23) 	HG2 :: 2.31(0.21) 	HG3 :: 2.31(0.21)



FLAG polypeptide(L)
1.P24	Overall: Anomalous 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Anomalous 	N Shifts: Indeterminable


	H Shift Assignments>> 	HA :: 2.264(S)	 	HB2 :: 1.035	 	HB3 :: -0.717(A)	 	HG2 :: 1.116	 	HG3 :: -0.673(S)	 	HD2 :: 2.985	
			 	HD3 :: 1.977(S)	
	Ave H Shift Values>> 	HA :: 4.41(0.36) 	HB2 :: 2.05(0.39) 	HB3 :: 2.05(0.39) 	HG2 :: 1.93(0.36) 	HG3 :: 1.92(0.37) 	HD2 :: 3.64(0.36)
			 	HD3 :: 3.63(0.4)



FLAG polypeptide(L)
1.S25	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 8.295	 	HA :: 4.243	 	HB2 :: 4.015	
	Ave H Shift Values>> 	H :: 8.29(0.62) 	HA :: 4.51(0.43) 	HB2 :: 3.88(0.29) 	HB3 :: 3.87(0.31) 	HG :: 5.33(1.16)



FLAG polypeptide(L)
1.K26	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 8.813	 	HA :: 4.369	 	HB2 :: 1.512	 	HB3 :: 1.708	 	HG2 :: 1.879	 	HE2 :: 3.050	
	Ave H Shift Values>> 	H :: 8.22(0.64) 	HA :: 4.28(0.45) 	HB2 :: 1.79(0.25) 	HB3 :: 1.78(0.26) 	HG2 :: 1.38(0.27) 	HG3 :: 1.37(0.29)
			 	HD2 :: 1.61(0.25) 	HD3 :: 1.61(0.25) 	HE2 :: 2.93(0.2) 	HE3 :: 2.93(0.2) 	HZ :: 7.52(0.36)



FLAG polypeptide(L)
1.D27	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 7.852	 	HA :: 4.833	 	HB2 :: 3.103	 	HB3 :: 2.653	
	Ave H Shift Values>> 	H :: 8.33(0.57) 	HA :: 4.61(0.31) 	HB2 :: 2.74(0.29) 	HB3 :: 2.7(0.29)



FLAG polypeptide(L)
1.G28	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 8.560	 	HA2 :: 4.397	 	HA3 :: 4.177	
	Ave H Shift Values>> 	H :: 8.34(0.74) 	HA2 :: 3.95(0.4) 	HA3 :: 3.93(0.4)



FLAG polypeptide(L)
1.A29	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 8.375	 	HA :: 4.391	 	HB1 :: 1.607	 	HB2 :: 1.607	 	HB3 :: 1.607	
	Ave H Shift Values>> 	H :: 8.2(0.6) 	HA :: 4.26(0.42) 	HB :: 1.38(0.25)



FLAG polypeptide(L)
1.Y30	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 8.661	 	HA :: 4.014	 	HB2 :: 3.123	 	HB3 :: 2.530	 	HD1 :: 6.911	 	HD2 :: 6.912	
			 	HE1 :: 6.332	
	Ave H Shift Values>> 	H :: 8.37(0.75) 	HA :: 4.63(0.56) 	HB2 :: 2.91(0.37) 	HB3 :: 2.91(0.37) 	HD1 :: 6.87(0.65) 	HD2 :: 6.86(0.68)
			 	HE1 :: 6.64(0.58) 	HE2 :: 6.63(0.61) 	HH :: 9.25(1.34)



FLAG polypeptide(L)
1.E31	Overall: Suspicious 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Suspicious 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 9.525	 	HA :: 4.238	 	HB2 :: 2.795(S)	 	HG2 :: 3.133(S)	
	Ave H Shift Values>> 	H :: 8.34(0.61) 	HA :: 4.26(0.43) 	HB2 :: 2.04(0.22) 	HB3 :: 2.04(0.23) 	HG2 :: 2.31(0.21) 	HG3 :: 2.31(0.21)



FLAG polypeptide(L)
1.F32	Overall: Anomalous 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Anomalous 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 8.247	 	HA :: 5.401	 	HB2 :: 4.178	 	HB3 :: 3.657	 	HD1 :: 8.026(A)	 	HE1 :: 7.815	
			 	HE2 :: 7.818	 	HZ :: 7.690	
	Ave H Shift Values>> 	H :: 8.42(0.74) 	HA :: 4.62(0.58) 	HB2 :: 2.99(0.37) 	HB3 :: 2.97(0.38) 	HD1 :: 6.91(0.82) 	HD2 :: 6.89(0.86)
			 	HE1 :: 6.95(0.82) 	HE2 :: 6.94(0.86) 	HZ :: 6.92(0.8)



FLAG polypeptide(L)
1.E33	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 8.353	 	HA :: 3.763	 	HB2 :: 2.134	 	HB3 :: 2.009	 	HG2 :: 2.357	 	HG3 :: 2.334	
	Ave H Shift Values>> 	H :: 8.34(0.61) 	HA :: 4.26(0.43) 	HB2 :: 2.04(0.22) 	HB3 :: 2.04(0.23) 	HG2 :: 2.31(0.21) 	HG3 :: 2.31(0.21)



FLAG polypeptide(L)
1.Q34	Overall: Suspicious 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Suspicious 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 8.408	 	HA :: 4.288	 	HB2 :: 3.029(S)	 	HB3 :: 2.845	 	HG2 :: 2.362	 	HG3 :: 2.222	
			 	HE21 :: 6.013	 	HE22 :: 6.608	
	Ave H Shift Values>> 	H :: 8.22(0.62) 	HA :: 4.28(0.44) 	HB2 :: 2.05(0.28) 	HB3 :: 2.04(0.29) 	HG2 :: 2.32(0.29) 	HG3 :: 2.32(0.29)
			 	HE21 :: 7.17(0.46) 	HE22 :: 7.07(0.49)



FLAG polypeptide(L)
1.C35	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 10.043	 	HA :: 5.048	 	HB2 :: 3.651	 	HB3 :: 3.561	
	Ave H Shift Values>> 	H :: 8.42(0.66) 	HA :: 4.73(0.57) 	HB2 :: 2.95(0.43) 	HB3 :: 2.98(0.45) 	HG :: 1.66(0.48)



FLAG polypeptide(L)
1.Q36	Overall: Suspicious 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Suspicious 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 7.667	 	HA :: 4.122	 	HB2 :: 1.692	 	HG2 :: 2.337	 	HG3 :: 1.981	 	HE21 :: 6.207	
			 	HE22 :: 5.039(S)	
	Ave H Shift Values>> 	H :: 8.22(0.62) 	HA :: 4.28(0.44) 	HB2 :: 2.05(0.28) 	HB3 :: 2.04(0.29) 	HG2 :: 2.32(0.29) 	HG3 :: 2.32(0.29)
			 	HE21 :: 7.17(0.46) 	HE22 :: 7.07(0.49)



FLAG polypeptide(L)
1.S37	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 8.069	 	HA :: 4.104	 	HB2 :: 4.009	
	Ave H Shift Values>> 	H :: 8.29(0.62) 	HA :: 4.51(0.43) 	HB2 :: 3.88(0.29) 	HB3 :: 3.87(0.31) 	HG :: 5.33(1.16)



FLAG polypeptide(L)
1.C38	Overall: Suspicious 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Suspicious 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 6.534	 	HA :: 4.458	 	HB2 :: 1.361(S)	 	HB3 :: 2.245	
	Ave H Shift Values>> 	H :: 8.42(0.66) 	HA :: 4.73(0.57) 	HB2 :: 2.95(0.43) 	HB3 :: 2.98(0.45) 	HG :: 1.66(0.48)



FLAG polypeptide(L)
1.H39	Overall: Anomalous 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Anomalous 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 7.195	 	HA :: 3.167	 	HB2 :: 1.800	 	HB3 :: 1.717	 	HD1 :: 9.543	 	HD2 :: 1.275(A)	
			 	HE1 :: 1.553(A)	
	Ave H Shift Values>> 	H :: 8.25(0.74) 	HA :: 4.62(0.51) 	HB2 :: 3.12(0.43) 	HB3 :: 3.11(0.45) 	HD1 :: 10.14(3.5) 	HD2 :: 7.08(0.5)
			 	HE1 :: 8.08(0.57) 	HE2 :: 10.43(3.11)



FLAG polypeptide(L)
1.G40	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 7.528	 	HA2 :: 3.838	 	HA3 :: 3.336	
	Ave H Shift Values>> 	H :: 8.34(0.74) 	HA2 :: 3.95(0.4) 	HA3 :: 3.93(0.4)



FLAG polypeptide(L)
1.S41	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 7.364	 	HA :: 4.332	 	HB2 :: 3.820	 	HB3 :: 3.596	
	Ave H Shift Values>> 	H :: 8.29(0.62) 	HA :: 4.51(0.43) 	HB2 :: 3.88(0.29) 	HB3 :: 3.87(0.31) 	HG :: 5.33(1.16)



FLAG polypeptide(L)
1.L42	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 8.713	 	HA :: 3.145	 	HB2 :: 1.521	 	HB3 :: 0.630	 	HG :: 1.756	 	HD11 :: 0.944	
			 	HD12 :: 0.944	 	HD13 :: 0.944	 	HD21 :: 0.507	 	HD22 :: 0.507	 	HD23 :: 0.507	
	Ave H Shift Values>> 	H :: 8.22(0.65) 	HA :: 4.32(0.47) 	HB2 :: 1.63(0.34) 	HB3 :: 1.57(0.36) 	HG :: 1.54(0.33) 	HD1 :: 0.77(0.27)
			 	HD2 :: 0.76(0.28)



FLAG polypeptide(L)
1.A43	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 7.277	 	HA :: 3.522	 	HB1 :: 1.091	 	HB2 :: 1.091	 	HB3 :: 1.091	
	Ave H Shift Values>> 	H :: 8.2(0.6) 	HA :: 4.26(0.42) 	HB :: 1.38(0.25)



FLAG polypeptide(L)
1.E44	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 6.953	 	HA :: 4.001	 	HB2 :: 1.615	 	HG2 :: 1.924	 	HG3 :: 1.846	
	Ave H Shift Values>> 	H :: 8.34(0.61) 	HA :: 4.26(0.43) 	HB2 :: 2.04(0.22) 	HB3 :: 2.04(0.23) 	HG2 :: 2.31(0.21) 	HG3 :: 2.31(0.21)



FLAG polypeptide(L)
1.M45	Overall: Suspicious 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Suspicious 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 6.301	 	HA :: 3.491	 	HB2 :: 0.380(S)	 	HB3 :: -0.224(S)	 	HG2 :: 0.756(S)	 	HG3 :: 1.802	
			 	HE1 :: 0.524	 	HE2 :: 0.524	 	HE3 :: 0.524	
	Ave H Shift Values>> 	H :: 8.26(0.6) 	HA :: 4.39(0.46) 	HB2 :: 2.03(0.38) 	HB3 :: 2.01(0.36) 	HG2 :: 2.44(0.36) 	HG3 :: 2.41(0.4)
			 	HE :: 1.86(0.47)



FLAG polypeptide(L)
1.D46	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 6.781	 	HA :: 3.893	 	HB2 :: 2.996	 	HB3 :: 2.110	
	Ave H Shift Values>> 	H :: 8.33(0.57) 	HA :: 4.61(0.31) 	HB2 :: 2.74(0.29) 	HB3 :: 2.7(0.29)



FLAG polypeptide(L)
1.D47	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 7.863	 	HA :: 3.940	 	HB2 :: 2.606	 	HB3 :: 2.319	
	Ave H Shift Values>> 	H :: 8.33(0.57) 	HA :: 4.61(0.31) 	HB2 :: 2.74(0.29) 	HB3 :: 2.7(0.29)



FLAG polypeptide(L)
1.N48	Overall: Suspicious 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Suspicious 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 7.316	 	HA :: 4.186	 	HB2 :: 1.585(S)	 	HB3 :: 1.946	 	HD21 :: 8.353	 	HD22 :: 7.441	
	Ave H Shift Values>> 	H :: 8.37(0.65) 	HA :: 4.7(0.39) 	HB2 :: 2.8(0.34) 	HB3 :: 2.78(0.34) 	HD21 :: 7.27(0.52) 	HD22 :: 7.2(0.52)



FLAG polypeptide(L)
1.H49	Overall: Anomalous 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Anomalous 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 6.197	 	HA :: 1.562(A)	 	HB2 :: 1.125(S)	 	HB3 :: 0.926(S)	 	HD1 :: 8.777	 	HD2 :: 0.153(A)	
			 	HE1 :: 1.225(A)	
	Ave H Shift Values>> 	H :: 8.25(0.74) 	HA :: 4.62(0.51) 	HB2 :: 3.12(0.43) 	HB3 :: 3.11(0.45) 	HD1 :: 10.14(3.5) 	HD2 :: 7.08(0.5)
			 	HE1 :: 8.08(0.57) 	HE2 :: 10.43(3.11)



FLAG polypeptide(L)
1.K50	Overall: Suspicious 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Suspicious 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 7.161	 	HA :: 3.125	 	HB2 :: 0.983	 	HB3 :: 0.916(S)	 	HG2 :: 1.218	 	HG3 :: 1.162	
			 	HE2 :: 2.772	
	Ave H Shift Values>> 	H :: 8.22(0.64) 	HA :: 4.28(0.45) 	HB2 :: 1.79(0.25) 	HB3 :: 1.78(0.26) 	HG2 :: 1.38(0.27) 	HG3 :: 1.37(0.29)
			 	HD2 :: 1.61(0.25) 	HD3 :: 1.61(0.25) 	HE2 :: 2.93(0.2) 	HE3 :: 2.93(0.2) 	HZ :: 7.52(0.36)



FLAG polypeptide(L)
1.P51	Overall: Anomalous 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Anomalous 	N Shifts: Indeterminable


	H Shift Assignments>> 	HA :: 3.694	 	HB2 :: 1.695	 	HB3 :: -0.896(A)	 	HG2 :: 1.476	 	HG3 :: 0.531(S)	 	HD2 :: 1.761(S)	
			 	HD3 :: 2.768	
	Ave H Shift Values>> 	HA :: 4.41(0.36) 	HB2 :: 2.05(0.39) 	HB3 :: 2.05(0.39) 	HG2 :: 1.93(0.36) 	HG3 :: 1.92(0.37) 	HD2 :: 3.64(0.36)
			 	HD3 :: 3.63(0.4)



FLAG polypeptide(L)
1.H52	Overall: Anomalous 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Anomalous 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 6.170	 	HA :: 2.763(S)	 	HB2 :: 1.832	 	HB3 :: 1.321(S)	 	HD1 :: 6.962	 	HD2 :: 0.897(A)	
			 	HE1 :: 1.323(A)	
	Ave H Shift Values>> 	H :: 8.25(0.74) 	HA :: 4.62(0.51) 	HB2 :: 3.12(0.43) 	HB3 :: 3.11(0.45) 	HD1 :: 10.14(3.5) 	HD2 :: 7.08(0.5)
			 	HE1 :: 8.08(0.57) 	HE2 :: 10.43(3.11)



FLAG polypeptide(L)
1.D53	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 6.870	 	HA :: 4.033	 	HB2 :: 2.361	 	HB3 :: 2.024	
	Ave H Shift Values>> 	H :: 8.33(0.57) 	HA :: 4.61(0.31) 	HB2 :: 2.74(0.29) 	HB3 :: 2.7(0.29)



FLAG polypeptide(L)
1.G54	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 8.685	 	HA2 :: 4.253	 	HA3 :: 3.660	
	Ave H Shift Values>> 	H :: 8.34(0.74) 	HA2 :: 3.95(0.4) 	HA3 :: 3.93(0.4)



FLAG polypeptide(L)
1.L55	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 7.965	 	HA :: 4.636	 	HB2 :: 1.423	 	HB3 :: 1.576	 	HD11 :: 1.050	 	HD12 :: 1.050	
			 	HD13 :: 1.050	 	HD21 :: 1.090	 	HD22 :: 1.090	 	HD23 :: 1.090	
	Ave H Shift Values>> 	H :: 8.22(0.65) 	HA :: 4.32(0.47) 	HB2 :: 1.63(0.34) 	HB3 :: 1.57(0.36) 	HG :: 1.54(0.33) 	HD1 :: 0.77(0.27)
			 	HD2 :: 0.76(0.28)



FLAG polypeptide(L)
1.L56	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 7.090	 	HA :: 4.894	 	HB2 :: 1.377	 	HB3 :: 1.920	 	HG :: 1.286	 	HD11 :: 0.369	
			 	HD12 :: 0.369	 	HD13 :: 0.369	 	HD21 :: 1.176	 	HD22 :: 1.176	 	HD23 :: 1.176	
	Ave H Shift Values>> 	H :: 8.22(0.65) 	HA :: 4.32(0.47) 	HB2 :: 1.63(0.34) 	HB3 :: 1.57(0.36) 	HG :: 1.54(0.33) 	HD1 :: 0.77(0.27)
			 	HD2 :: 0.76(0.28)



FLAG polypeptide(L)
1.M57	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 9.052	 	HA :: 4.032	 	HB2 :: 1.803	 	HB3 :: 1.376	 	HG2 :: 2.456	
	Ave H Shift Values>> 	H :: 8.26(0.6) 	HA :: 4.39(0.46) 	HB2 :: 2.03(0.38) 	HB3 :: 2.01(0.36) 	HG2 :: 2.44(0.36) 	HG3 :: 2.41(0.4)
			 	HE :: 1.86(0.47)



FLAG polypeptide(L)
1.C58	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 7.800	 	HA :: 5.550	 	HB2 :: 3.600	 	HB3 :: 3.411	
	Ave H Shift Values>> 	H :: 8.42(0.66) 	HA :: 4.73(0.57) 	HB2 :: 2.95(0.43) 	HB3 :: 2.98(0.45) 	HG :: 1.66(0.48)



FLAG polypeptide(L)
1.A59	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 9.149	 	HA :: 4.072	 	HB1 :: 1.983	 	HB2 :: 1.983	 	HB3 :: 1.983	
	Ave H Shift Values>> 	H :: 8.2(0.6) 	HA :: 4.26(0.42) 	HB :: 1.38(0.25)



FLAG polypeptide(L)
1.D60	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 7.878	 	HA :: 4.543	 	HB2 :: 3.157	 	HB3 :: 2.909	
	Ave H Shift Values>> 	H :: 8.33(0.57) 	HA :: 4.61(0.31) 	HB2 :: 2.74(0.29) 	HB3 :: 2.7(0.29)



FLAG polypeptide(L)
1.C61	Overall: Suspicious 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Suspicious 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 6.557	 	HA :: 4.728	 	HB2 :: 1.858	 	HB3 :: 1.371(S)	
	Ave H Shift Values>> 	H :: 8.42(0.66) 	HA :: 4.73(0.57) 	HB2 :: 2.95(0.43) 	HB3 :: 2.98(0.45) 	HG :: 1.66(0.48)



FLAG polypeptide(L)
1.H62	Overall: Anomalous 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Anomalous 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 7.267	 	HA :: 3.031	 	HB2 :: 1.503(S)	 	HB3 :: 1.639	 	HD1 :: 9.817	 	HD2 :: 0.849(A)	
			 	HE1 :: 1.322(A)	
	Ave H Shift Values>> 	H :: 8.25(0.74) 	HA :: 4.62(0.51) 	HB2 :: 3.12(0.43) 	HB3 :: 3.11(0.45) 	HD1 :: 10.14(3.5) 	HD2 :: 7.08(0.5)
			 	HE1 :: 8.08(0.57) 	HE2 :: 10.43(3.11)



FLAG polypeptide(L)
1.A63	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 7.251	 	HA :: 4.202	 	HB1 :: 1.077	 	HB2 :: 1.077	 	HB3 :: 1.077	
	Ave H Shift Values>> 	H :: 8.2(0.6) 	HA :: 4.26(0.42) 	HB :: 1.38(0.25)



FLAG polypeptide(L)
1.P64	Overall: Suspicious 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Suspicious 	N Shifts: Indeterminable


	H Shift Assignments>> 	HA :: 3.270	 	HB2 :: -0.449(S)	 	HB3 :: 0.644(S)	 	HG2 :: 2.124	 	HG3 :: 1.502	 	HD2 :: 3.222	
			 	HD3 :: 4.075	
	Ave H Shift Values>> 	HA :: 4.41(0.36) 	HB2 :: 2.05(0.39) 	HB3 :: 2.05(0.39) 	HG2 :: 1.93(0.36) 	HG3 :: 1.92(0.37) 	HD2 :: 3.64(0.36)
			 	HD3 :: 3.63(0.4)



FLAG polypeptide(L)
1.H65	Overall: Anomalous 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Anomalous 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 7.148	 	HA :: 2.937(S)	 	HB2 :: 1.152(S)	 	HB3 :: 1.558(S)	 	HD1 :: 8.629	 	HD2 :: 0.837(A)	
			 	HE1 :: 1.160(A)	
	Ave H Shift Values>> 	H :: 8.25(0.74) 	HA :: 4.62(0.51) 	HB2 :: 3.12(0.43) 	HB3 :: 3.11(0.45) 	HD1 :: 10.14(3.5) 	HD2 :: 7.08(0.5)
			 	HE1 :: 8.08(0.57) 	HE2 :: 10.43(3.11)



FLAG polypeptide(L)
1.E66	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 7.113	 	HA :: 3.936	 	HB2 :: 1.704	 	HB3 :: 1.476	 	HG2 :: 1.809	
	Ave H Shift Values>> 	H :: 8.34(0.61) 	HA :: 4.26(0.43) 	HB2 :: 2.04(0.22) 	HB3 :: 2.04(0.23) 	HG2 :: 2.31(0.21) 	HG3 :: 2.31(0.21)



FLAG polypeptide(L)
1.A67	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 7.983	 	HA :: 3.852	 	HB1 :: 0.817	 	HB2 :: 0.817	 	HB3 :: 0.817	
	Ave H Shift Values>> 	H :: 8.2(0.6) 	HA :: 4.26(0.42) 	HB :: 1.38(0.25)



FLAG polypeptide(L)
1.K68	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 7.981	 	HA :: 4.293	 	HB2 :: 1.283	 	HB3 :: 1.226	 	HG2 :: 1.075	 	HG3 :: 1.211	
	Ave H Shift Values>> 	H :: 8.22(0.64) 	HA :: 4.28(0.45) 	HB2 :: 1.79(0.25) 	HB3 :: 1.78(0.26) 	HG2 :: 1.38(0.27) 	HG3 :: 1.37(0.29)
			 	HD2 :: 1.61(0.25) 	HD3 :: 1.61(0.25) 	HE2 :: 2.93(0.2) 	HE3 :: 2.93(0.2) 	HZ :: 7.52(0.36)



FLAG polypeptide(L)
1.V69	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 7.859	 	HA :: 3.207	 	HB :: 1.493	 	HG11 :: 0.446	 	HG12 :: 0.446	 	HG13 :: 0.446	
			 	HG21 :: 0.271	 	HG22 :: 0.271	 	HG23 :: 0.271	
	Ave H Shift Values>> 	H :: 8.29(0.71) 	HA :: 4.16(0.57) 	HB :: 1.99(0.33) 	HG1 :: 0.84(0.27) 	HG2 :: 0.83(0.29)



FLAG polypeptide(L)
1.G70	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 8.901	 	HA2 :: 4.234	 	HA3 :: 3.817	
	Ave H Shift Values>> 	H :: 8.34(0.74) 	HA2 :: 3.95(0.4) 	HA3 :: 3.93(0.4)



FLAG polypeptide(L)
1.E71	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 7.596	 	HA :: 4.437	 	HB2 :: 2.065	 	HB3 :: 1.889	
	Ave H Shift Values>> 	H :: 8.34(0.61) 	HA :: 4.26(0.43) 	HB2 :: 2.04(0.22) 	HB3 :: 2.04(0.23) 	HG2 :: 2.31(0.21) 	HG3 :: 2.31(0.21)



FLAG polypeptide(L)
1.K72	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 8.483	 	HA :: 4.571	 	HB2 :: 2.075	 	HG2 :: 1.889	
	Ave H Shift Values>> 	H :: 8.22(0.64) 	HA :: 4.28(0.45) 	HB2 :: 1.79(0.25) 	HB3 :: 1.78(0.26) 	HG2 :: 1.38(0.27) 	HG3 :: 1.37(0.29)
			 	HD2 :: 1.61(0.25) 	HD3 :: 1.61(0.25) 	HE2 :: 2.93(0.2) 	HE3 :: 2.93(0.2) 	HZ :: 7.52(0.36)



FLAG polypeptide(L)
1.P73	Overall: Anomalous 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Anomalous 	N Shifts: Indeterminable


	H Shift Assignments>> 	HA :: 4.419	 	HB2 :: 2.193	 	HB3 :: 2.336	 	HG2 :: -1.125(A)	 	HG3 :: 0.123(S)	 	HD2 :: 2.554	
			 	HD3 :: 2.456	
	Ave H Shift Values>> 	HA :: 4.41(0.36) 	HB2 :: 2.05(0.39) 	HB3 :: 2.05(0.39) 	HG2 :: 1.93(0.36) 	HG3 :: 1.92(0.37) 	HD2 :: 3.64(0.36)
			 	HD3 :: 3.63(0.4)



FLAG polypeptide(L)
1.T74	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 8.511	 	HA :: 5.336	 	HB :: 4.748	 	HG21 :: 1.512	 	HG22 :: 1.512	 	HG23 :: 1.512	
	Ave H Shift Values>> 	H :: 8.27(0.62) 	HA :: 4.48(0.5) 	HB :: 4.17(0.37) 	HG1 :: 4.4(1.92) 	HG2 :: 1.16(0.28) 	HG21 :: 1.16(0.28)
			 	HG22 :: 1.16(0.28) 	HG23 :: 1.16(0.28)



FLAG polypeptide(L)
1.C75	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 8.998	 	HA :: 5.967	 	HB2 :: 2.748	 	HB3 :: 2.688	
	Ave H Shift Values>> 	H :: 8.42(0.66) 	HA :: 4.73(0.57) 	HB2 :: 2.95(0.43) 	HB3 :: 2.98(0.45) 	HG :: 1.66(0.48)



FLAG polypeptide(L)
1.D76	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 8.504	 	HA :: 4.565	 	HB2 :: 2.725	
	Ave H Shift Values>> 	H :: 8.33(0.57) 	HA :: 4.61(0.31) 	HB2 :: 2.74(0.29) 	HB3 :: 2.7(0.29)



FLAG polypeptide(L)
1.T77	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 7.809	 	HA :: 3.983	 	HB :: 4.580	 	HG21 :: 1.581	 	HG22 :: 1.581	 	HG23 :: 1.581	
	Ave H Shift Values>> 	H :: 8.27(0.62) 	HA :: 4.48(0.5) 	HB :: 4.17(0.37) 	HG1 :: 4.4(1.92) 	HG2 :: 1.16(0.28) 	HG21 :: 1.16(0.28)
			 	HG22 :: 1.16(0.28) 	HG23 :: 1.16(0.28)



FLAG polypeptide(L)
1.C78	Overall: Suspicious 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Suspicious 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 6.634	 	HA :: 4.427	 	HB2 :: 2.432	 	HB3 :: 0.969(S)	
	Ave H Shift Values>> 	H :: 8.42(0.66) 	HA :: 4.73(0.57) 	HB2 :: 2.95(0.43) 	HB3 :: 2.98(0.45) 	HG :: 1.66(0.48)



FLAG polypeptide(L)
1.H79	Overall: Anomalous 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Anomalous 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 7.043	 	HA :: 3.437	 	HB2 :: 1.181(S)	 	HB3 :: 1.324(S)	 	HD1 :: 8.803	 	HD2 :: 0.771(A)	
			 	HE1 :: 1.221(A)	
	Ave H Shift Values>> 	H :: 8.25(0.74) 	HA :: 4.62(0.51) 	HB2 :: 3.12(0.43) 	HB3 :: 3.11(0.45) 	HD1 :: 10.14(3.5) 	HD2 :: 7.08(0.5)
			 	HE1 :: 8.08(0.57) 	HE2 :: 10.43(3.11)



FLAG polypeptide(L)
1.D80	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 7.826	 	HA :: 4.464	 	HB2 :: 2.606	 	HB3 :: 2.342	
	Ave H Shift Values>> 	H :: 8.33(0.57) 	HA :: 4.61(0.31) 	HB2 :: 2.74(0.29) 	HB3 :: 2.7(0.29)



FLAG polypeptide(L)
1.D81	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 7.139	 	HA :: 4.321	 	HB2 :: 2.826	 	HB3 :: 2.271	
	Ave H Shift Values>> 	H :: 8.33(0.57) 	HA :: 4.61(0.31) 	HB2 :: 2.74(0.29) 	HB3 :: 2.7(0.29)



FLAG polypeptide(L)
1.G82	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 8.545	 	HA2 :: 3.871	 	HA3 :: 3.534	
	Ave H Shift Values>> 	H :: 8.34(0.74) 	HA2 :: 3.95(0.4) 	HA3 :: 3.93(0.4)



FLAG polypeptide(L)
1.R83	Overall: Suspicious 	Typing: Indeterminable 	SRO: Suspicious 	C Shifts: Indeterminable 	H Shifts: Suspicious 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 6.845	 	HA :: 4.250	 	HB2 :: 0.532(S)	 	HB3 :: 1.025	 	HG2 :: -0.341(S)	 	HG3 :: 2.042	
			 	HD2 :: 1.802(S)	 	HD3 :: 1.688(S)	 	HE :: 7.574	
	Ave H Shift Values>> 	H :: 8.24(0.6) 	HA :: 4.27(0.44) 	HB2 :: 1.79(0.28) 	HB3 :: 1.78(0.28) 	HG2 :: 1.58(0.27) 	HG3 :: 1.58(0.27)
			 	HD2 :: 3.13(0.23) 	HD3 :: 3.13(0.22) 	HE :: 7.32(0.52) 	HH11 :: 6.74(0.31) 	HH12 :: 6.75(0.32) 	HH21 :: 6.72(0.32)
			 	HH22 :: 6.72(0.33)

	SRO Rule Breaks>>  	(HD2 > HG3 : 0.9983) 	(HD3 > HG3 : 0.9982)


FLAG polypeptide(L)
1.T84	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 8.063	 	HA :: 5.059	 	HB :: 4.541	 	HG21 :: 1.321	 	HG22 :: 1.321	 	HG23 :: 1.321	
	Ave H Shift Values>> 	H :: 8.27(0.62) 	HA :: 4.48(0.5) 	HB :: 4.17(0.37) 	HG1 :: 4.4(1.92) 	HG2 :: 1.16(0.28) 	HG21 :: 1.16(0.28)
			 	HG22 :: 1.16(0.28) 	HG23 :: 1.16(0.28)



FLAG polypeptide(L)
1.A85	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 8.904	 	HA :: 4.736	 	HB1 :: 1.941	 	HB2 :: 1.941	 	HB3 :: 1.941	
	Ave H Shift Values>> 	H :: 8.2(0.6) 	HA :: 4.26(0.42) 	HB :: 1.38(0.25)



FLAG polypeptide(L)
1.K86	Overall: Consistent 	Typing: Indeterminable 	SRO: Consistent 	C Shifts: Indeterminable 	H Shifts: Consistent 	N Shifts: Indeterminable


	H Shift Assignments>> 	H :: 9.487	 	HA :: 4.307	 	HB2 :: 1.803	 	HB3 :: 1.711	 	HG2 :: 1.943	
	Ave H Shift Values>> 	H :: 8.22(0.64) 	HA :: 4.28(0.45) 	HB2 :: 1.79(0.25) 	HB3 :: 1.78(0.26) 	HG2 :: 1.38(0.27) 	HG3 :: 1.37(0.29)
			 	HD2 :: 1.61(0.25) 	HD3 :: 1.61(0.25) 	HE2 :: 2.93(0.2) 	HE3 :: 2.93(0.2) 	HZ :: 7.52(0.36)



FLAG 
2.HEM_red1 Overall Status: Residue type HEM_red not supported.





###########################################################################

Overall Results:

	      #Consistent:  58      #Clerical:   0      #Suspicious:  15      #Anomalous:  13      #Mistyped:   0      #Indeterminable:   0


Typing Results:

	      #Consistent:   0      #Suspicious:   0      #Mistyped:   0      #Indeterminable:  86


Shift Relative Order (SRO) Results:

	      #Consistent:  85      #Suspicious:   1


C Shift Results:

	      #Consistent:   0      #Duplicate:   0      #Unknown:   0      #Suspicious:   0      #Anomalous:   0


H Shift Results:

	      #Consistent: 406      #Duplicate:   0      #Unknown:   0      #Suspicious:  44      #Anomalous:  24


N Shift Results:

	      #Consistent:   0      #Duplicate:   0      #Unknown:   0      #Suspicious:   0      #Anomalous:   0



Error Summary:

1.L5	HG = -0.152(S),	 Expected =  1.54, Std = 0.3300, ChiSquare = 2.9397e-07
1.A6	HA = 2.662(S),	 Expected =  4.26, Std = 0.4200, ChiSquare = 1.4194e-04
1.A6	HB1 = -1.578(A),	 Expected =  1.38, Std = 0.2500, ChiSquare = 2.6671e-32
1.A6	HB2 = -1.578(A),	 Expected =  1.38, Std = 0.2500, ChiSquare = 2.6671e-32
1.A6	HB3 = -1.578(A),	 Expected =  1.38, Std = 0.2500, ChiSquare = 2.6671e-32
1.H9	HA = 2.781(S),	 Expected =  4.62, Std = 0.5100, ChiSquare = 3.1109e-04
1.H9	HD2 = 1.101(A),	 Expected =  7.08, Std = 0.5000, ChiSquare = 5.8965e-33
1.H9	HE1 = 1.309(A),	 Expected =  8.08, Std = 0.5700, ChiSquare = 1.5227e-32
1.C18	HB2 = 0.629(S),	 Expected =  2.95, Std = 0.4300, ChiSquare = 6.7510e-08
1.H19	HB2 = 0.573(S),	 Expected =  3.12, Std = 0.4300, ChiSquare = 3.1563e-09
1.H19	HB3 = 0.797(S),	 Expected =  3.11, Std = 0.4500, ChiSquare = 2.7474e-07
1.H19	HD2 = 0.139(A),	 Expected =  7.08, Std = 0.5000, ChiSquare = 8.1439e-44
1.H19	HE1 = 0.867(A),	 Expected =  8.08, Std = 0.5700, ChiSquare = 1.0580e-36
1.A20	HB1 = 0.236(S),	 Expected =  1.38, Std = 0.2500, ChiSquare = 4.7395e-06
1.A20	HB2 = 0.236(S),	 Expected =  1.38, Std = 0.2500, ChiSquare = 4.7395e-06
1.A20	HB3 = 0.236(S),	 Expected =  1.38, Std = 0.2500, ChiSquare = 4.7395e-06
1.G22	HA3 = 2.380(S),	 Expected =  3.93, Std = 0.4000, ChiSquare = 1.0662e-04
1.P24	HA = 2.264(S),	 Expected =  4.41, Std = 0.3600, ChiSquare = 2.5053e-09
1.P24	HB3 = -0.717(A),	 Expected =  2.05, Std = 0.3900, ChiSquare = 1.2947e-12
1.P24	HG3 = -0.673(S),	 Expected =  1.92, Std = 0.3700, ChiSquare = 2.4156e-12
1.P24	HD3 = 1.977(S),	 Expected =  3.63, Std = 0.4000, ChiSquare = 3.5884e-05
1.E31	HB2 = 2.795(S),	 Expected =  2.04, Std = 0.2200, ChiSquare = 5.9955e-04
1.E31	HG2 = 3.133(S),	 Expected =  2.31, Std = 0.2100, ChiSquare = 8.8900e-05
1.F32	HD1 = 8.026(A),	 Expected =  6.91, Std = 0.8200, ChiSquare = 1.7352e-01
1.Q34	HB2 = 3.029(S),	 Expected =  2.05, Std = 0.2800, ChiSquare = 4.7153e-04
1.Q36	HE22 = 5.039(S),	 Expected =  7.07, Std = 0.4900, ChiSquare = 3.3997e-05
1.C38	HB2 = 1.361(S),	 Expected =  2.95, Std = 0.4300, ChiSquare = 2.1959e-04
1.H39	HD2 = 1.275(A),	 Expected =  7.08, Std = 0.5000, ChiSquare = 3.6662e-31
1.H39	HE1 = 1.553(A),	 Expected =  8.08, Std = 0.5700, ChiSquare = 2.3278e-30
1.M45	HB2 = 0.380(S),	 Expected =  2.03, Std = 0.3800, ChiSquare = 1.4112e-05
1.M45	HB3 = -0.224(S),	 Expected =  2.01, Std = 0.3600, ChiSquare = 5.4504e-10
1.M45	HG2 = 0.756(S),	 Expected =  2.44, Std = 0.3600, ChiSquare = 2.9000e-06
1.N48	HB2 = 1.585(S),	 Expected =  2.80, Std = 0.3400, ChiSquare = 3.5220e-04
1.H49	HA = 1.562(A),	 Expected =  4.62, Std = 0.5100, ChiSquare = 2.0214e-09
1.H49	HB2 = 1.125(S),	 Expected =  3.12, Std = 0.4300, ChiSquare = 3.4919e-06
1.H49	HB3 = 0.926(S),	 Expected =  3.11, Std = 0.4500, ChiSquare = 1.2140e-06
1.H49	HD2 = 0.153(A),	 Expected =  7.08, Std = 0.5000, ChiSquare = 1.2032e-43
1.H49	HE1 = 1.225(A),	 Expected =  8.08, Std = 0.5700, ChiSquare = 2.5844e-33
1.K50	HB3 = 0.916(S),	 Expected =  1.78, Std = 0.2600, ChiSquare = 8.9030e-04
1.P51	HB3 = -0.896(A),	 Expected =  2.05, Std = 0.3900, ChiSquare = 4.2259e-14
1.P51	HG3 = 0.531(S),	 Expected =  1.92, Std = 0.3700, ChiSquare = 1.7400e-04
1.P51	HD2 = 1.761(S),	 Expected =  3.64, Std = 0.3600, ChiSquare = 1.7946e-07
1.H52	HA = 2.763(S),	 Expected =  4.62, Std = 0.5100, ChiSquare = 2.7140e-04
1.H52	HB3 = 1.321(S),	 Expected =  3.11, Std = 0.4500, ChiSquare = 7.0215e-05
1.H52	HD2 = 0.897(A),	 Expected =  7.08, Std = 0.5000, ChiSquare = 3.9921e-35
1.H52	HE1 = 1.323(A),	 Expected =  8.08, Std = 0.5700, ChiSquare = 2.0422e-32
1.C61	HB3 = 1.371(S),	 Expected =  2.98, Std = 0.4500, ChiSquare = 3.4948e-04
1.H62	HB2 = 1.503(S),	 Expected =  3.12, Std = 0.4300, ChiSquare = 1.6960e-04
1.H62	HD2 = 0.849(A),	 Expected =  7.08, Std = 0.5000, ChiSquare = 1.2031e-35
1.H62	HE1 = 1.322(A),	 Expected =  8.08, Std = 0.5700, ChiSquare = 1.9998e-32
1.P64	HB2 = -0.449(S),	 Expected =  2.05, Std = 0.3900, ChiSquare = 1.4774e-10
1.P64	HB3 = 0.644(S),	 Expected =  2.05, Std = 0.3900, ChiSquare = 3.1200e-04
1.H65	HA = 2.937(S),	 Expected =  4.62, Std = 0.5100, ChiSquare = 9.6685e-04
1.H65	HB2 = 1.152(S),	 Expected =  3.12, Std = 0.4300, ChiSquare = 4.7227e-06
1.H65	HB3 = 1.558(S),	 Expected =  3.11, Std = 0.4500, ChiSquare = 5.6290e-04
1.H65	HD2 = 0.837(A),	 Expected =  7.08, Std = 0.5000, ChiSquare = 8.9018e-36
1.H65	HE1 = 1.160(A),	 Expected =  8.08, Std = 0.5700, ChiSquare = 6.4549e-34
1.P73	HG2 = -1.125(A),	 Expected =  1.93, Std = 0.3600, ChiSquare = 2.1367e-17
1.P73	HG3 = 0.123(S),	 Expected =  1.92, Std = 0.3700, ChiSquare = 1.1932e-06
1.C78	HB3 = 0.969(S),	 Expected =  2.98, Std = 0.4500, ChiSquare = 7.8627e-06
1.H79	HB2 = 1.181(S),	 Expected =  3.12, Std = 0.4300, ChiSquare = 6.5041e-06
1.H79	HB3 = 1.324(S),	 Expected =  3.11, Std = 0.4500, ChiSquare = 7.2209e-05
1.H79	HD2 = 0.771(A),	 Expected =  7.08, Std = 0.5000, ChiSquare = 1.6803e-36
1.H79	HE1 = 1.221(A),	 Expected =  8.08, Std = 0.5700, ChiSquare = 2.3738e-33
1.R83	HB2 = 0.532(S),	 Expected =  1.79, Std = 0.2800, ChiSquare = 7.0274e-06
1.R83	HG2 = -0.341(S),	 Expected =  1.58, Std = 0.2700, ChiSquare = 1.1206e-12
1.R83	HD2 = 1.802(S),	 Expected =  3.13, Std = 0.2300, ChiSquare = 7.7451e-09
1.R83	HD3 = 1.688(S),	 Expected =  3.13, Std = 0.2200, ChiSquare = 5.5812e-11
1.R83	SRO Rule Break>>  HD2 > HG3 : 0.998289867464728
1.R83	SRO Rule Break>>  HD3 > HG3 : 0.998244844229925