data_15833 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; NMR CHEMICAL SHIFT ASSIGNMENTS OF PUTATIVE UNCHARACTERIZED PROTEIN. FROM METHANOCALDOCOCCUS JANNASCHII, NORTHEAST STRUCTURAL GENOMICS CONSORTIUM (NESG) TARGET GsR195 ; _BMRB_accession_number 15833 _BMRB_flat_file_name bmr15833.str _Entry_type original _Submission_date 2008-06-27 _Accession_date 2008-06-27 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 LIU GAOHUA . . 2 ZHAO LI . COLL 3 CICCOSANTI COLLEEN . . 4 JIANG MEI . . 5 XIAO RONG . . 6 SWAPNA G.V.T . . 7 NAIR RAJESH . . 8 EVERETT JOHN K. . 9 ACTON THOMAS B. . 10 ROST BURKHARD . . 11 Montelione GAETANO T. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 375 "13C chemical shifts" 207 "15N chemical shifts" 77 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2010-05-27 update BMRB 'update entity/assembly name' 2009-06-09 update BMRB 'added time domain data' 2009-02-11 update BMRB 'correct residue numbers in data tables' 2008-08-22 original author 'original release' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title N.A. _Citation_status 'in preparation' _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 LIU GAOHUA . . 2 XIAO RONG . . 3 Montelione GAETANO . . stop_ _Journal_abbreviation 'Not known' _Journal_volume . _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first . _Page_last . _Year . _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name GsR195 _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label GsR195 $entity stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_entity _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common GsR195 _Molecular_mass 16006.114 _Mol_thiol_state 'all free' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 146 _Mol_residue_sequence ; MTQKFTKDMTFAQALQTHPG VAGVLRSYNLGCIGCMGAQN ESLEQGANAHGLNVEDILRD LNALALEHHHHHHMTQKFTK DMTFAQALQTHPGVAGVLRS YNLGCIGCMGAQNESLEQGA NAHGLNVEDILRDLNALALE HHHHHH ; loop_ _Residue_seq_code _Residue_label 1 MET 2 THR 3 GLN 4 LYS 5 PHE 6 THR 7 LYS 8 ASP 9 MET 10 THR 11 PHE 12 ALA 13 GLN 14 ALA 15 LEU 16 GLN 17 THR 18 HIS 19 PRO 20 GLY 21 VAL 22 ALA 23 GLY 24 VAL 25 LEU 26 ARG 27 SER 28 TYR 29 ASN 30 LEU 31 GLY 32 CYS 33 ILE 34 GLY 35 CYS 36 MET 37 GLY 38 ALA 39 GLN 40 ASN 41 GLU 42 SER 43 LEU 44 GLU 45 GLN 46 GLY 47 ALA 48 ASN 49 ALA 50 HIS 51 GLY 52 LEU 53 ASN 54 VAL 55 GLU 56 ASP 57 ILE 58 LEU 59 ARG 60 ASP 61 LEU 62 ASN 63 ALA 64 LEU 65 ALA 66 LEU 67 GLU 68 HIS 69 HIS 70 HIS 71 HIS 72 HIS 73 HIS 74 MET 75 THR 76 GLN 77 LYS 78 PHE 79 THR 80 LYS 81 ASP 82 MET 83 THR 84 PHE 85 ALA 86 GLN 87 ALA 88 LEU 89 GLN 90 THR 91 HIS 92 PRO 93 GLY 94 VAL 95 ALA 96 GLY 97 VAL 98 LEU 99 ARG 100 SER 101 TYR 102 ASN 103 LEU 104 GLY 105 CYS 106 ILE 107 GLY 108 CYS 109 MET 110 GLY 111 ALA 112 GLN 113 ASN 114 GLU 115 SER 116 LEU 117 GLU 118 GLN 119 GLY 120 ALA 121 ASN 122 ALA 123 HIS 124 GLY 125 LEU 126 ASN 127 VAL 128 GLU 129 ASP 130 ILE 131 LEU 132 ARG 133 ASP 134 LEU 135 ASN 136 ALA 137 LEU 138 ALA 139 LEU 140 GLU 141 HIS 142 HIS 143 HIS 144 HIS 145 HIS 146 HIS stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2014-06-12 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 2K5E "Solution Structure Of Putative Uncharacterized Protein Gsu1278 From Methanocaldococcus Jannaschii, Northeast Structural Genomic" 50.00 73 100.00 100.00 5.13e-44 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $entity 'Methanococcus jannaschii' 2190 Archaea . Methanococcus jannaschii stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $entity 'recombinant technology' . Escherichia coli . 'pET 21-23C' stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details ; 10% D2O, 50 uM DSS and protease inhibitors added NMR buffer pH 4.5 ; loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity 1.11 mM '[U-100% 13C; U-100% 15N]' DSS 50 uM 'natural abundance' H2O 90 % 'natural abundance' D2O 10 % '[U-100% 2H]' stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type solution _Details 'NMR buffer pH 4.5,10% D2O, 50 uM DSS, 1x protease inhibitors.' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity 1.48 mM '[U-10% 13C; U-100% 15N]' DSS 50 uM 'natural abundance' H2O 90 % 'natural abundance' D2O 10 % '[U-100% 2H]' stop_ save_ ############################ # Computer software used # ############################ save_CNS _Saveframe_category software _Name CNS _Version 1.2 loop_ _Vendor _Address _Electronic_address 'Brunger, Adams, Clore, Gros, Nilges and Read' . . stop_ loop_ _Task refinement stop_ _Details . save_ save_CYANA _Saveframe_category software _Name CYANA _Version 2.1 loop_ _Vendor _Address _Electronic_address 'Guntert, Mumenthaler and Wuthrich' . . stop_ loop_ _Task 'geometry optimization' refinement 'structure solution' stop_ _Details . save_ save_AutoStructure _Saveframe_category software _Name AutoStruct _Version 2.2.1 loop_ _Vendor _Address _Electronic_address 'Huang, Tejero, Powers and Montelione' . . stop_ loop_ _Task 'data analysis' 'peak picking' stop_ _Details . save_ save_AutoAssign _Saveframe_category software _Name AutoAssign _Version 2.3.0 loop_ _Vendor _Address _Electronic_address 'Zimmerman, Moseley, Kulikowski and Montelione' . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version 2.3 loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ save_XEASY _Saveframe_category software _Name XEASY _Version 1.3.1 loop_ _Vendor _Address _Electronic_address 'Bartels et al.' . . stop_ loop_ _Task 'data analysis' stop_ _Details . save_ save_TOPSPIN _Saveframe_category software _Name TOPSPIN _Version . loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . stop_ loop_ _Task collection stop_ _Details . save_ save_VNMRJ _Saveframe_category software _Name VNMRJ _Version . loop_ _Vendor _Address _Electronic_address Varian . . stop_ loop_ _Task collection stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 800 _Details . save_ save_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model INOVA _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_2D_1H-13C_HSQC_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HSQC' _Sample_label $sample_1 save_ save_2D_1H-13C_HSQC_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HSQC' _Sample_label $sample_2 save_ save_3D_SIMULTANEOUS_CN-NOESY_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D SIMULTANEOUS CN-NOESY' _Sample_label $sample_1 save_ save_4,3D_GFT_CABCACONHN_5 _Saveframe_category NMR_applied_experiment _Experiment_name '4,3D GFT CABCACONHN' _Sample_label $sample_1 save_ save_4,3D_GFT_HNNCABCA_6 _Saveframe_category NMR_applied_experiment _Experiment_name '4,3D GFT HNNCABCA' _Sample_label $sample_1 save_ save_4,3D_GFT_HABCABCONHN_7 _Saveframe_category NMR_applied_experiment _Experiment_name '4,3D GFT HABCABCONHN' _Sample_label $sample_1 save_ save_3D_HNCO_8 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO' _Sample_label $sample_1 save_ save_3D_CCH-COSY_9 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CCH-COSY' _Sample_label $sample_1 save_ save_NMR_spectrometer_expt_1 _Saveframe_category NMR_applied_experiment _Experiment_name '3D SIMULTANEOUS CN-NOESY' _BMRB_pulse_sequence_accession_number . _Details . save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 4.5 . pH pressure 1 . atm temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio _Indirect_shift_ratio_citation_label _Correction_value_citation_label DSS C 13 'methyl protons' ppm 0.00 . indirect . . . 0.251449530 $entry_citation $entry_citation DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 $entry_citation $entry_citation DSS N 15 'methyl protons' ppm 0.00 . indirect . . . 0.101329118 $entry_citation $entry_citation stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Software_label $CYANA stop_ loop_ _Experiment_label '3D SIMULTANEOUS CN-NOESY' '4,3D GFT CABCACONHN' '4,3D GFT HNNCABCA' '4,3D GFT HABCABCONHN' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name GsR195 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 2 2 THR HA H 3.83 . 1 2 2 2 THR HB H 4.04 . 1 3 2 2 THR HG2 H 1.26 . 1 4 2 2 THR CA C 61.3 . 1 5 2 2 THR CB C 68.9 . 1 6 2 2 THR CG2 C 21.4 . 1 7 3 3 GLN H H 8.74 . 1 8 3 3 GLN HA H 4.31 . 1 9 3 3 GLN HB2 H 2.00 . 2 10 3 3 GLN HB3 H 1.86 . 2 11 3 3 GLN HE21 H 6.86 . 2 12 3 3 GLN HE22 H 7.50 . 2 13 3 3 GLN HG2 H 2.25 . 2 14 3 3 GLN HG3 H 2.25 . 2 15 3 3 GLN CA C 55.6 . 1 16 3 3 GLN CB C 29.2 . 1 17 3 3 GLN CG C 33.5 . 1 18 3 3 GLN N N 124.8 . 1 19 3 3 GLN NE2 N 112.4 . 1 20 4 4 LYS H H 8.27 . 1 21 4 4 LYS HA H 4.05 . 1 22 4 4 LYS HB2 H 1.63 . 2 23 4 4 LYS HB3 H 1.53 . 2 24 4 4 LYS HD2 H 1.56 . 2 25 4 4 LYS HD3 H 1.56 . 2 26 4 4 LYS HE2 H 2.90 . 2 27 4 4 LYS HE3 H 2.90 . 2 28 4 4 LYS HG2 H 1.21 . 2 29 4 4 LYS HG3 H 1.25 . 2 30 4 4 LYS CA C 56.5 . 1 31 4 4 LYS CB C 33.1 . 1 32 4 4 LYS CD C 28.5 . 1 33 4 4 LYS CE C 41.8 . 1 34 4 4 LYS CG C 24.5 . 1 35 4 4 LYS N N 122.2 . 1 36 5 5 PHE H H 8.91 . 1 37 5 5 PHE HA H 4.79 . 1 38 5 5 PHE HB2 H 2.61 . 2 39 5 5 PHE HB3 H 2.51 . 2 40 5 5 PHE HD1 H 7.18 . 3 41 5 5 PHE HD2 H 7.18 . 3 42 5 5 PHE HE1 H 7.28 . 3 43 5 5 PHE HE2 H 7.28 . 3 44 5 5 PHE HZ H 6.85 . 1 45 5 5 PHE CA C 57.4 . 1 46 5 5 PHE CB C 39.6 . 1 47 5 5 PHE CD1 C 132.5 . 1 48 5 5 PHE CE1 C 131.0 . 1 49 5 5 PHE CZ C 129.1 . 1 50 5 5 PHE N N 117.9 . 1 51 6 6 THR H H 7.65 . 1 52 6 6 THR HA H 4.65 . 1 53 6 6 THR HB H 4.40 . 1 54 6 6 THR HG2 H 1.06 . 1 55 6 6 THR CA C 59.2 . 1 56 6 6 THR CB C 71.6 . 1 57 6 6 THR CG2 C 21.6 . 1 58 6 6 THR N N 112.8 . 1 59 7 7 LYS H H 8.99 . 1 60 7 7 LYS HA H 3.78 . 1 61 7 7 LYS HB2 H 1.76 . 2 62 7 7 LYS HB3 H 1.68 . 2 63 7 7 LYS HD2 H 1.58 . 2 64 7 7 LYS HD3 H 1.44 . 2 65 7 7 LYS HE2 H 2.90 . 2 66 7 7 LYS HE3 H 2.96 . 2 67 7 7 LYS HG2 H 1.31 . 2 68 7 7 LYS HG3 H 1.37 . 2 69 7 7 LYS CA C 58.6 . 1 70 7 7 LYS CB C 32.8 . 1 71 7 7 LYS CD C 29.3 . 1 72 7 7 LYS CE C 41.7 . 1 73 7 7 LYS CG C 23.3 . 1 74 7 7 LYS N N 117.9 . 1 75 8 8 ASP H H 7.92 . 1 76 8 8 ASP HA H 4.64 . 1 77 8 8 ASP HB2 H 2.56 . 2 78 8 8 ASP HB3 H 2.71 . 2 79 8 8 ASP CA C 53.8 . 1 80 8 8 ASP CB C 41.0 . 1 81 8 8 ASP N N 113.6 . 1 82 9 9 MET H H 7.24 . 1 83 9 9 MET HA H 4.28 . 1 84 9 9 MET HB2 H 1.98 . 2 85 9 9 MET HB3 H 2.25 . 2 86 9 9 MET HE H 1.88 . 1 87 9 9 MET HG2 H 2.67 . 2 88 9 9 MET HG3 H 2.72 . 2 89 9 9 MET CA C 55.7 . 1 90 9 9 MET CB C 33.8 . 1 91 9 9 MET CE C 15.5 . 1 92 9 9 MET CG C 30.4 . 1 93 9 9 MET N N 121.6 . 1 94 10 10 THR H H 8.41 . 1 95 10 10 THR HA H 4.91 . 1 96 10 10 THR HB H 4.81 . 1 97 10 10 THR HG1 H 5.78 . 1 98 10 10 THR HG2 H 1.14 . 1 99 10 10 THR CA C 60.3 . 1 100 10 10 THR CB C 70.8 . 1 101 10 10 THR CG2 C 21.6 . 1 102 10 10 THR N N 110.9 . 1 103 11 11 PHE H H 8.25 . 1 104 11 11 PHE HA H 3.87 . 1 105 11 11 PHE HB2 H 2.86 . 2 106 11 11 PHE HB3 H 3.29 . 2 107 11 11 PHE HD1 H 7.26 . 3 108 11 11 PHE HD2 H 7.26 . 3 109 11 11 PHE HE1 H 7.19 . 3 110 11 11 PHE HE2 H 7.19 . 3 111 11 11 PHE HZ H 6.99 . 1 112 11 11 PHE CA C 63.1 . 1 113 11 11 PHE CB C 39.2 . 1 114 11 11 PHE CD1 C 131.6 . 1 115 11 11 PHE CE1 C 131.4 . 1 116 11 11 PHE CZ C 127.8 . 1 117 11 11 PHE N N 119.2 . 1 118 12 12 ALA H H 8.56 . 1 119 12 12 ALA HA H 4.10 . 1 120 12 12 ALA HB H 1.37 . 1 121 12 12 ALA CA C 55.3 . 1 122 12 12 ALA CB C 18.2 . 1 123 12 12 ALA N N 119.1 . 1 124 13 13 GLN H H 7.94 . 1 125 13 13 GLN HA H 3.91 . 1 126 13 13 GLN HB2 H 2.28 . 2 127 13 13 GLN HB3 H 1.89 . 2 128 13 13 GLN HE21 H 7.46 . 2 129 13 13 GLN HE22 H 6.82 . 2 130 13 13 GLN HG2 H 2.34 . 2 131 13 13 GLN HG3 H 2.39 . 2 132 13 13 GLN CA C 58.1 . 1 133 13 13 GLN CB C 28.4 . 1 134 13 13 GLN CG C 33.9 . 1 135 13 13 GLN N N 117.2 . 1 136 13 13 GLN NE2 N 111.2 . 1 137 14 14 ALA H H 8.90 . 1 138 14 14 ALA HA H 4.23 . 1 139 14 14 ALA HB H 1.58 . 1 140 14 14 ALA CA C 56.0 . 1 141 14 14 ALA CB C 17.3 . 1 142 14 14 ALA N N 123.9 . 1 143 15 15 LEU H H 8.83 . 1 144 15 15 LEU HA H 3.91 . 1 145 15 15 LEU HB2 H 1.54 . 2 146 15 15 LEU HB3 H 1.97 . 2 147 15 15 LEU HD1 H 0.90 . 1 148 15 15 LEU HD2 H 0.95 . 1 149 15 15 LEU HG H 1.71 . 1 150 15 15 LEU CA C 58.0 . 1 151 15 15 LEU CB C 42.2 . 1 152 15 15 LEU CD1 C 25.3 . 1 153 15 15 LEU CD2 C 23.3 . 1 154 15 15 LEU CG C 27.7 . 1 155 15 15 LEU N N 118.4 . 1 156 16 16 GLN H H 7.24 . 1 157 16 16 GLN HA H 4.25 . 1 158 16 16 GLN HB2 H 2.09 . 2 159 16 16 GLN HB3 H 2.16 . 2 160 16 16 GLN HE21 H 6.77 . 2 161 16 16 GLN HE22 H 7.34 . 2 162 16 16 GLN HG2 H 2.48 . 2 163 16 16 GLN HG3 H 2.48 . 2 164 16 16 GLN CA C 56.0 . 1 165 16 16 GLN CB C 29.0 . 1 166 16 16 GLN CG C 33.6 . 1 167 16 16 GLN N N 113.3 . 1 168 16 16 GLN NE2 N 111.5 . 1 169 17 17 THR H H 7.96 . 1 170 17 17 THR HA H 3.92 . 1 171 17 17 THR HB H 4.44 . 1 172 17 17 THR HG2 H 0.76 . 1 173 17 17 THR CA C 66.4 . 1 174 17 17 THR CB C 68.8 . 1 175 17 17 THR CG2 C 21.4 . 1 176 17 17 THR N N 116.1 . 1 177 18 18 HIS H H 7.15 . 1 178 18 18 HIS HA H 4.88 . 1 179 18 18 HIS HB2 H 2.97 . 2 180 18 18 HIS HB3 H 3.25 . 2 181 18 18 HIS HD2 H 7.24 . 1 182 18 18 HIS CA C 55.5 . 1 183 18 18 HIS CB C 34.0 . 1 184 18 18 HIS CD2 C 118.5 . 1 185 18 18 HIS CE1 C 135.8 . 1 186 18 18 HIS N N 119.2 . 1 187 19 19 PRO HA H 4.59 . 1 188 19 19 PRO HB2 H 1.97 . 2 189 19 19 PRO HB3 H 2.33 . 2 190 19 19 PRO HD2 H 2.43 . 2 191 19 19 PRO HD3 H 3.48 . 2 192 19 19 PRO HG2 H 1.75 . 2 193 19 19 PRO HG3 H 1.83 . 2 194 19 19 PRO CA C 64.6 . 1 195 19 19 PRO CB C 31.8 . 1 196 19 19 PRO CD C 50.8 . 1 197 19 19 PRO CG C 27.0 . 1 198 20 20 GLY H H 8.46 . 1 199 20 20 GLY HA2 H 3.89 . 2 200 20 20 GLY HA3 H 4.29 . 2 201 20 20 GLY CA C 45.4 . 1 202 20 20 GLY N N 112.1 . 1 203 21 21 VAL H H 7.88 . 1 204 21 21 VAL HA H 3.19 . 1 205 21 21 VAL HB H 2.40 . 1 206 21 21 VAL HG1 H 0.72 . 1 207 21 21 VAL HG2 H 1.31 . 1 208 21 21 VAL CA C 66.7 . 1 209 21 21 VAL CB C 31.3 . 1 210 21 21 VAL CG1 C 20.2 . 1 211 21 21 VAL CG2 C 24.8 . 1 212 21 21 VAL N N 122.8 . 1 213 22 22 ALA H H 8.42 . 1 214 22 22 ALA HA H 3.83 . 1 215 22 22 ALA HB H 1.42 . 1 216 22 22 ALA CA C 55.9 . 1 217 22 22 ALA CB C 17.3 . 1 218 22 22 ALA N N 121.9 . 1 219 23 23 GLY H H 8.46 . 1 220 23 23 GLY HA2 H 3.72 . 2 221 23 23 GLY HA3 H 3.89 . 2 222 23 23 GLY CA C 46.6 . 1 223 23 23 GLY N N 105.3 . 1 224 24 24 VAL H H 7.41 . 1 225 24 24 VAL HA H 3.79 . 1 226 24 24 VAL HB H 2.07 . 1 227 24 24 VAL HG1 H 0.89 . 1 228 24 24 VAL HG2 H 0.93 . 1 229 24 24 VAL CA C 66.0 . 1 230 24 24 VAL CB C 31.5 . 1 231 24 24 VAL CG1 C 21.8 . 1 232 24 24 VAL CG2 C 22.3 . 1 233 24 24 VAL N N 123.1 . 1 234 25 25 LEU H H 8.63 . 1 235 25 25 LEU HA H 3.98 . 1 236 25 25 LEU HB2 H 1.36 . 2 237 25 25 LEU HB3 H 1.92 . 2 238 25 25 LEU HD1 H 0.85 . 1 239 25 25 LEU HD2 H 0.62 . 1 240 25 25 LEU HG H 1.86 . 1 241 25 25 LEU CA C 58.4 . 1 242 25 25 LEU CB C 39.3 . 1 243 25 25 LEU CD1 C 26.1 . 1 244 25 25 LEU CD2 C 21.2 . 1 245 25 25 LEU CG C 26.0 . 1 246 25 25 LEU N N 118.6 . 1 247 26 26 ARG H H 8.45 . 1 248 26 26 ARG HA H 4.08 . 1 249 26 26 ARG HB2 H 1.91 . 2 250 26 26 ARG HB3 H 1.95 . 2 251 26 26 ARG HD2 H 3.17 . 2 252 26 26 ARG HD3 H 3.21 . 2 253 26 26 ARG HE H 7.26 . 1 254 26 26 ARG HG2 H 1.78 . 2 255 26 26 ARG HG3 H 1.59 . 2 256 26 26 ARG CA C 59.6 . 1 257 26 26 ARG CB C 29.8 . 1 258 26 26 ARG CD C 43.2 . 1 259 26 26 ARG CG C 27.8 . 1 260 26 26 ARG N N 119.5 . 1 261 26 26 ARG NE N 84.2 . 1 262 27 27 SER H H 7.78 . 1 263 27 27 SER HA H 4.20 . 1 264 27 27 SER HB2 H 3.89 . 2 265 27 27 SER HB3 H 3.85 . 2 266 27 27 SER CA C 61.3 . 1 267 27 27 SER CB C 62.4 . 1 268 27 27 SER N N 118.0 . 1 269 28 28 TYR H H 7.24 . 1 270 28 28 TYR HA H 4.35 . 1 271 28 28 TYR HB2 H 3.34 . 2 272 28 28 TYR HB3 H 2.43 . 2 273 28 28 TYR HD1 H 7.18 . 3 274 28 28 TYR HD2 H 7.18 . 3 275 28 28 TYR HE1 H 6.72 . 3 276 28 28 TYR HE2 H 6.72 . 3 277 28 28 TYR CA C 59.5 . 1 278 28 28 TYR CB C 39.0 . 1 279 28 28 TYR CD1 C 133.4 . 1 280 28 28 TYR CE1 C 117.2 . 1 281 28 28 TYR N N 119.5 . 1 282 29 29 ASN H H 8.14 . 1 283 29 29 ASN HA H 4.45 . 1 284 29 29 ASN HB2 H 2.80 . 2 285 29 29 ASN HB3 H 3.12 . 2 286 29 29 ASN HD21 H 6.85 . 2 287 29 29 ASN HD22 H 7.56 . 2 288 29 29 ASN CA C 54.1 . 1 289 29 29 ASN CB C 36.9 . 1 290 29 29 ASN N N 115.2 . 1 291 29 29 ASN ND2 N 112.7 . 1 292 30 30 LEU H H 7.71 . 1 293 30 30 LEU HA H 4.15 . 1 294 30 30 LEU HB2 H 1.42 . 2 295 30 30 LEU HB3 H 1.47 . 2 296 30 30 LEU HD1 H 0.68 . 1 297 30 30 LEU HD2 H 0.37 . 1 298 30 30 LEU HG H 1.43 . 1 299 30 30 LEU CA C 53.4 . 1 300 30 30 LEU CB C 42.6 . 1 301 30 30 LEU CD1 C 25.8 . 1 302 30 30 LEU CD2 C 22.5 . 1 303 30 30 LEU CG C 25.6 . 1 304 30 30 LEU N N 116.7 . 1 305 31 31 GLY H H 8.13 . 1 306 31 31 GLY HA2 H 3.72 . 2 307 31 31 GLY HA3 H 3.78 . 2 308 31 31 GLY CA C 45.4 . 1 309 31 31 GLY N N 107.7 . 1 310 32 32 CYS H H 8.09 . 1 311 32 32 CYS HA H 4.40 . 1 312 32 32 CYS HB2 H 2.77 . 2 313 32 32 CYS HB3 H 2.77 . 2 314 32 32 CYS CA C 57.9 . 1 315 32 32 CYS CB C 27.4 . 1 316 32 32 CYS N N 117.5 . 1 317 33 33 ILE H H 7.81 . 1 318 33 33 ILE HA H 3.88 . 1 319 33 33 ILE HB H 1.65 . 1 320 33 33 ILE HD1 H 0.58 . 1 321 33 33 ILE HG12 H 1.20 . 2 322 33 33 ILE HG13 H 1.00 . 2 323 33 33 ILE HG2 H 0.61 . 1 324 33 33 ILE CA C 61.7 . 1 325 33 33 ILE CB C 37.6 . 1 326 33 33 ILE CD1 C 12.6 . 1 327 33 33 ILE CG1 C 27.1 . 1 328 33 33 ILE CG2 C 17.4 . 1 329 33 33 ILE N N 122.3 . 1 330 34 34 GLY H H 8.40 . 1 331 34 34 GLY HA2 H 3.82 . 2 332 34 34 GLY HA3 H 3.90 . 2 333 34 34 GLY CA C 45.5 . 1 334 34 34 GLY N N 111.3 . 1 335 35 35 CYS H H 7.93 . 1 336 35 35 CYS HA H 4.48 . 1 337 35 35 CYS HB2 H 2.89 . 2 338 35 35 CYS HB3 H 2.89 . 2 339 35 35 CYS CA C 58.0 . 1 340 35 35 CYS CB C 27.7 . 1 341 35 35 CYS N N 118.0 . 1 342 36 36 MET H H 8.40 . 1 343 36 36 MET HA H 4.35 . 1 344 36 36 MET HB2 H 2.58 . 2 345 36 36 MET HB3 H 2.01 . 2 346 36 36 MET HE H 1.99 . 1 347 36 36 MET HG2 H 2.06 . 2 348 36 36 MET HG3 H 2.49 . 2 349 36 36 MET CA C 56.4 . 1 350 36 36 MET CB C 31.9 . 1 351 36 36 MET CE C 17.0 . 1 352 36 36 MET CG C 31.9 . 1 353 36 36 MET N N 121.5 . 1 354 37 37 GLY H H 8.34 . 1 355 37 37 GLY HA2 H 3.85 . 2 356 37 37 GLY HA3 H 3.92 . 2 357 37 37 GLY CA C 45.8 . 1 358 37 37 GLY N N 109.1 . 1 359 38 38 ALA H H 7.89 . 1 360 38 38 ALA HA H 4.31 . 1 361 38 38 ALA HB H 1.30 . 1 362 38 38 ALA CA C 52.8 . 1 363 38 38 ALA CB C 18.7 . 1 364 38 38 ALA N N 123.3 . 1 365 39 39 GLN H H 8.32 . 1 366 39 39 GLN HA H 4.16 . 1 367 39 39 GLN HB2 H 2.02 . 2 368 39 39 GLN HB3 H 2.12 . 2 369 39 39 GLN HE21 H 7.36 . 2 370 39 39 GLN HE22 H 6.69 . 2 371 39 39 GLN HG2 H 2.33 . 2 372 39 39 GLN HG3 H 2.39 . 2 373 39 39 GLN CA C 57.5 . 1 374 39 39 GLN CB C 28.2 . 1 375 39 39 GLN CG C 33.4 . 1 376 39 39 GLN N N 116.8 . 1 377 39 39 GLN NE2 N 111.2 . 1 378 40 40 ASN H H 8.12 . 1 379 40 40 ASN HA H 4.91 . 1 380 40 40 ASN HB2 H 2.75 . 2 381 40 40 ASN HB3 H 2.96 . 2 382 40 40 ASN HD21 H 7.63 . 2 383 40 40 ASN HD22 H 6.89 . 2 384 40 40 ASN CA C 52.7 . 1 385 40 40 ASN CB C 38.8 . 1 386 40 40 ASN N N 115.5 . 1 387 40 40 ASN ND2 N 113.3 . 1 388 41 41 GLU H H 7.70 . 1 389 41 41 GLU HA H 4.53 . 1 390 41 41 GLU HB2 H 2.15 . 2 391 41 41 GLU HB3 H 2.15 . 2 392 41 41 GLU HG2 H 2.59 . 2 393 41 41 GLU HG3 H 2.54 . 2 394 41 41 GLU CA C 55.4 . 1 395 41 41 GLU CB C 30.0 . 1 396 41 41 GLU CG C 33.6 . 1 397 41 41 GLU N N 120.5 . 1 398 42 42 SER H H 8.68 . 1 399 42 42 SER HA H 4.71 . 1 400 42 42 SER HB2 H 3.89 . 2 401 42 42 SER HB3 H 4.38 . 2 402 42 42 SER CA C 56.6 . 1 403 42 42 SER CB C 65.6 . 1 404 42 42 SER N N 117.3 . 1 405 43 43 LEU H H 8.42 . 1 406 43 43 LEU HA H 3.54 . 1 407 43 43 LEU HB2 H 1.65 . 2 408 43 43 LEU HB3 H 0.73 . 2 409 43 43 LEU HD1 H 0.50 . 1 410 43 43 LEU HD2 H -0.15 . 1 411 43 43 LEU HG H 1.42 . 1 412 43 43 LEU CA C 57.7 . 1 413 43 43 LEU CB C 41.3 . 1 414 43 43 LEU CD1 C 25.8 . 1 415 43 43 LEU CD2 C 21.8 . 1 416 43 43 LEU CG C 26.6 . 1 417 43 43 LEU N N 119.9 . 1 418 44 44 GLU H H 8.26 . 1 419 44 44 GLU HA H 3.69 . 1 420 44 44 GLU HB2 H 1.86 . 2 421 44 44 GLU HB3 H 1.94 . 2 422 44 44 GLU HG2 H 2.23 . 2 423 44 44 GLU HG3 H 2.10 . 2 424 44 44 GLU CA C 59.6 . 1 425 44 44 GLU CB C 29.6 . 1 426 44 44 GLU CG C 35.4 . 1 427 44 44 GLU N N 116.8 . 1 428 45 45 GLN H H 8.16 . 1 429 45 45 GLN HA H 4.05 . 1 430 45 45 GLN HB2 H 2.07 . 2 431 45 45 GLN HB3 H 2.37 . 2 432 45 45 GLN HE21 H 7.51 . 2 433 45 45 GLN HE22 H 6.86 . 2 434 45 45 GLN HG2 H 2.44 . 2 435 45 45 GLN HG3 H 2.47 . 2 436 45 45 GLN CA C 58.9 . 1 437 45 45 GLN CB C 28.2 . 1 438 45 45 GLN CG C 34.1 . 1 439 45 45 GLN N N 119.9 . 1 440 45 45 GLN NE2 N 111.5 . 1 441 46 46 GLY H H 8.89 . 1 442 46 46 GLY HA2 H 3.82 . 2 443 46 46 GLY HA3 H 4.12 . 2 444 46 46 GLY CA C 47.2 . 1 445 46 46 GLY N N 107.0 . 1 446 47 47 ALA H H 8.89 . 1 447 47 47 ALA HA H 3.95 . 1 448 47 47 ALA HB H 1.28 . 1 449 47 47 ALA CA C 55.8 . 1 450 47 47 ALA CB C 17.4 . 1 451 47 47 ALA N N 124.4 . 1 452 48 48 ASN H H 8.73 . 1 453 48 48 ASN HA H 4.62 . 1 454 48 48 ASN HB2 H 2.84 . 2 455 48 48 ASN HB3 H 2.93 . 2 456 48 48 ASN HD21 H 7.02 . 2 457 48 48 ASN HD22 H 7.61 . 2 458 48 48 ASN CA C 55.8 . 1 459 48 48 ASN CB C 37.6 . 1 460 48 48 ASN N N 118.1 . 1 461 48 48 ASN ND2 N 111.1 . 1 462 49 49 ALA H H 8.12 . 1 463 49 49 ALA HA H 4.17 . 1 464 49 49 ALA HB H 1.46 . 1 465 49 49 ALA CA C 53.9 . 1 466 49 49 ALA CB C 18.3 . 1 467 49 49 ALA N N 121.7 . 1 468 50 50 HIS H H 7.51 . 1 469 50 50 HIS HA H 4.75 . 1 470 50 50 HIS HB2 H 2.78 . 2 471 50 50 HIS HB3 H 3.77 . 2 472 50 50 HIS HD2 H 7.58 . 1 473 50 50 HIS CA C 55.1 . 1 474 50 50 HIS CB C 29.5 . 1 475 50 50 HIS CD2 C 120.5 . 1 476 50 50 HIS CE1 C 136.3 . 1 477 50 50 HIS N N 112.7 . 1 478 51 51 GLY H H 7.82 . 1 479 51 51 GLY HA2 H 3.99 . 2 480 51 51 GLY HA3 H 3.99 . 2 481 51 51 GLY CA C 47.0 . 1 482 51 51 GLY N N 109.4 . 1 483 52 52 LEU H H 8.16 . 1 484 52 52 LEU HA H 4.60 . 1 485 52 52 LEU HB2 H 1.36 . 2 486 52 52 LEU HB3 H 1.50 . 2 487 52 52 LEU HD1 H 1.06 . 1 488 52 52 LEU HD2 H 1.07 . 1 489 52 52 LEU HG H 1.67 . 1 490 52 52 LEU CA C 52.7 . 1 491 52 52 LEU CB C 44.5 . 1 492 52 52 LEU CD1 C 26.8 . 1 493 52 52 LEU CD2 C 22.3 . 1 494 52 52 LEU CG C 26.4 . 1 495 52 52 LEU N N 118.7 . 1 496 53 53 ASN H H 8.81 . 1 497 53 53 ASN HA H 4.81 . 1 498 53 53 ASN HB2 H 3.12 . 2 499 53 53 ASN HB3 H 2.73 . 2 500 53 53 ASN HD21 H 6.99 . 2 501 53 53 ASN HD22 H 7.59 . 2 502 53 53 ASN CA C 51.3 . 1 503 53 53 ASN CB C 38.0 . 1 504 53 53 ASN N N 119.3 . 1 505 53 53 ASN ND2 N 112.4 . 1 506 54 54 VAL H H 9.34 . 1 507 54 54 VAL HA H 3.48 . 1 508 54 54 VAL HB H 2.08 . 1 509 54 54 VAL HG1 H 0.90 . 1 510 54 54 VAL HG2 H 0.72 . 1 511 54 54 VAL CA C 64.2 . 1 512 54 54 VAL CB C 31.5 . 1 513 54 54 VAL CG1 C 20.0 . 1 514 54 54 VAL CG2 C 21.8 . 1 515 54 54 VAL N N 127.6 . 1 516 55 55 GLU H H 8.25 . 1 517 55 55 GLU HA H 3.86 . 1 518 55 55 GLU HB2 H 1.85 . 2 519 55 55 GLU HB3 H 1.97 . 2 520 55 55 GLU HG2 H 2.22 . 2 521 55 55 GLU HG3 H 2.37 . 2 522 55 55 GLU CA C 58.8 . 1 523 55 55 GLU CB C 28.4 . 1 524 55 55 GLU CG C 35.6 . 1 525 55 55 GLU N N 119.3 . 1 526 56 56 ASP H H 7.42 . 1 527 56 56 ASP HA H 4.25 . 1 528 56 56 ASP HB2 H 2.79 . 2 529 56 56 ASP HB3 H 3.00 . 2 530 56 56 ASP CA C 57.2 . 1 531 56 56 ASP CB C 39.3 . 1 532 56 56 ASP N N 120.7 . 1 533 57 57 ILE H H 6.35 . 1 534 57 57 ILE HA H 3.05 . 1 535 57 57 ILE HB H 1.39 . 1 536 57 57 ILE HD1 H 0.33 . 1 537 57 57 ILE HG12 H -0.52 . 2 538 57 57 ILE HG13 H 0.78 . 2 539 57 57 ILE HG2 H 0.31 . 1 540 57 57 ILE CA C 64.6 . 1 541 57 57 ILE CB C 37.7 . 1 542 57 57 ILE CD1 C 14.2 . 1 543 57 57 ILE CG1 C 26.2 . 1 544 57 57 ILE CG2 C 16.0 . 1 545 57 57 ILE N N 118.2 . 1 546 58 58 LEU H H 8.35 . 1 547 58 58 LEU HA H 3.56 . 1 548 58 58 LEU HB2 H 1.73 . 2 549 58 58 LEU HB3 H 1.18 . 2 550 58 58 LEU HD1 H 0.70 . 1 551 58 58 LEU HD2 H 0.37 . 1 552 58 58 LEU HG H 1.59 . 1 553 58 58 LEU CA C 57.4 . 1 554 58 58 LEU CB C 41.0 . 1 555 58 58 LEU CD1 C 26.0 . 1 556 58 58 LEU CD2 C 22.4 . 1 557 58 58 LEU CG C 25.7 . 1 558 58 58 LEU N N 116.8 . 1 559 59 59 ARG H H 7.72 . 1 560 59 59 ARG HA H 3.94 . 1 561 59 59 ARG HB2 H 1.94 . 2 562 59 59 ARG HB3 H 2.00 . 2 563 59 59 ARG HD2 H 3.21 . 2 564 59 59 ARG HD3 H 3.21 . 2 565 59 59 ARG HE H 7.01 . 1 566 59 59 ARG HG2 H 1.39 . 2 567 59 59 ARG HG3 H 1.74 . 2 568 59 59 ARG CA C 59.4 . 1 569 59 59 ARG CB C 29.3 . 1 570 59 59 ARG CD C 43.5 . 1 571 59 59 ARG CG C 27.1 . 1 572 59 59 ARG N N 120.4 . 1 573 59 59 ARG NE N 84.5 . 1 574 60 60 ASP H H 7.28 . 1 575 60 60 ASP HA H 4.38 . 1 576 60 60 ASP HB2 H 2.70 . 2 577 60 60 ASP HB3 H 2.58 . 2 578 60 60 ASP CA C 56.8 . 1 579 60 60 ASP CB C 40.6 . 1 580 60 60 ASP N N 119.5 . 1 581 61 61 LEU H H 8.29 . 1 582 61 61 LEU HA H 3.59 . 1 583 61 61 LEU HB2 H -0.50 . 2 584 61 61 LEU HB3 H 1.06 . 2 585 61 61 LEU HD1 H 0.27 . 1 586 61 61 LEU HD2 H 0.34 . 1 587 61 61 LEU HG H 1.58 . 1 588 61 61 LEU CA C 56.7 . 1 589 61 61 LEU CB C 39.4 . 1 590 61 61 LEU CD1 C 26.4 . 1 591 61 61 LEU CD2 C 24.3 . 1 592 61 61 LEU CG C 25.4 . 1 593 61 61 LEU N N 120.2 . 1 594 62 62 ASN H H 8.80 . 1 595 62 62 ASN HA H 4.71 . 1 596 62 62 ASN HB2 H 2.77 . 2 597 62 62 ASN HB3 H 2.82 . 2 598 62 62 ASN HD21 H 7.05 . 2 599 62 62 ASN HD22 H 7.50 . 2 600 62 62 ASN CA C 56.0 . 1 601 62 62 ASN CB C 37.7 . 1 602 62 62 ASN N N 115.7 . 1 603 62 62 ASN ND2 N 114.4 . 1 604 63 63 ALA H H 7.42 . 1 605 63 63 ALA HA H 4.23 . 1 606 63 63 ALA HB H 1.45 . 1 607 63 63 ALA CA C 53.3 . 1 608 63 63 ALA CB C 18.2 . 1 609 63 63 ALA N N 121.3 . 1 610 64 64 LEU H H 7.33 . 1 611 64 64 LEU HA H 3.85 . 1 612 64 64 LEU HB2 H 0.32 . 2 613 64 64 LEU HB3 H 1.56 . 2 614 64 64 LEU HD1 H 0.52 . 1 615 64 64 LEU HD2 H 0.64 . 1 616 64 64 LEU HG H 1.70 . 1 617 64 64 LEU CA C 56.6 . 1 618 64 64 LEU CB C 40.5 . 1 619 64 64 LEU CD1 C 25.4 . 1 620 64 64 LEU CD2 C 22.3 . 1 621 64 64 LEU CG C 25.6 . 1 622 64 64 LEU N N 118.9 . 1 623 65 65 ALA H H 7.71 . 1 624 65 65 ALA HA H 4.29 . 1 625 65 65 ALA HB H 1.45 . 1 626 65 65 ALA CA C 52.2 . 1 627 65 65 ALA CB C 19.1 . 1 628 65 65 ALA N N 119.4 . 1 629 66 66 LEU H H 7.62 . 1 630 66 66 LEU HA H 4.27 . 1 631 66 66 LEU HB2 H 1.63 . 2 632 66 66 LEU HB3 H 1.50 . 2 633 66 66 LEU HD1 H 0.89 . 1 634 66 66 LEU HD2 H 0.83 . 1 635 66 66 LEU HG H 1.64 . 1 636 66 66 LEU CA C 54.9 . 1 637 66 66 LEU CB C 42.2 . 1 638 66 66 LEU CD1 C 24.8 . 1 639 66 66 LEU CD2 C 23.0 . 1 640 66 66 LEU CG C 26.8 . 1 641 66 66 LEU N N 120.1 . 1 642 67 67 GLU H H 8.10 . 1 643 67 67 GLU HA H 4.15 . 1 644 67 67 GLU HB2 H 1.89 . 2 645 67 67 GLU HB3 H 1.89 . 2 646 67 67 GLU HG2 H 2.16 . 2 647 67 67 GLU HG3 H 2.23 . 2 648 67 67 GLU CA C 56.3 . 1 649 67 67 GLU CB C 29.7 . 1 650 67 67 GLU CG C 35.6 . 1 651 67 67 GLU N N 120.9 . 1 652 68 68 HIS H H 8.55 . 1 653 68 68 HIS HA H 4.63 . 1 654 68 68 HIS HB2 H 3.16 . 2 655 68 68 HIS HB3 H 3.06 . 2 656 68 68 HIS HD2 H 7.21 . 1 657 68 68 HIS CA C 55.1 . 1 658 68 68 HIS CB C 28.8 . 1 659 68 68 HIS N N 119.5 . 1 stop_ save_