data_15907 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; 1J coupling constants related to the Ca carbons in human Ubiquitin ; _BMRB_accession_number 15907 _BMRB_flat_file_name bmr15907.str _Entry_type original _Submission_date 2008-08-04 _Accession_date 2008-08-04 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Schmidt Jurgen M. . 2 Lohr Frank . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count coupling_constants 4 stop_ loop_ _Data_type _Data_type_count "coupling constants" 265 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2009-01-29 update author 'complete entry citation' 2008-11-19 original author 'original release' stop_ loop_ _Related_BMRB_accession_number _Relationship 15410 'Backbone 1H, 13C, and 15N Chemical Shift Assignments for human ubiquitin' 15904 '1J coupling constants related to the Ca carbons in oxidised Flavodxin' 15905 '1J coupling constants related to the Ca carbons in Ribonuclease T1' 15906 '1J coupling constants related to the Ca carbons in Frataxin C-terminal domain' 15908 '1J coupling constants related to the Ca carbons in Xylanase' 15909 '1J coupling constants related to the Ca carbons in DFPase' 6466 '1H, 15N, 13C chemical-shift assignments for human Ubiquitin' 6488 '3J(HA-N) and 3J(HN-CO) coupling constants in Ubiquitin' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Variation in protein C(alpha)-related one-bond J couplings' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 18853398 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Schmidt Jurgen M. . 2 Howard Mark J. . 3 Maestre-Martinez Mitcheell . . 4 Perez Carlos S. . 5 Lohr Frank . . stop_ _Journal_abbreviation 'Magn. Reson. Chem.' _Journal_name_full 'Magnetic Resonance in Chemistry' _Journal_volume 47 _Journal_issue 1 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 16 _Page_last 30 _Year 2009 _Details . loop_ _Keyword 'data mining' 'one-bond coupling' statistics 'torsion angles' stop_ save_ ####################################### # Cited references within the entry # ####################################### save_Wang_et_al_1995 _Saveframe_category citation _Citation_full . _Citation_title 'Sequential backbone assignment of isotopically enriched proteins in D2O by deuterium-decoupled HA(CA)N and HA(CACO)N' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 7647557 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Wang A. C. . 2 Grzesiek S. . . 3 Tschudin R. . . 4 Lodi P. J. . 5 Bax A. . . stop_ _Journal_abbreviation 'J. Biomol. NMR' _Journal_name_full . _Journal_volume 5 _Journal_issue . _Journal_CSD . _Book_title . _Book_chapter_title . _Book_volume . _Book_series . _Book_publisher . _Book_publisher_city . _Book_ISBN . _Conference_title . _Conference_site . _Conference_state_province . _Conference_country . _Conference_start_date . _Conference_end_date . _Conference_abstract_number . _Thesis_institution . _Thesis_institution_city . _Thesis_institution_country . _Page_first 376 _Page_last 382 _Year 1995 _Details . loop_ _Keyword '1JCAN measurement in proline' stop_ save_ save_Salzmann_et_al_1998 _Saveframe_category citation _Citation_full . _Citation_title 'TROSY in triple-resonance experiments: New perspectives for sequential NMR assignment of large proteins' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 9811843 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Salzmann M. . . 2 Pervushin K. . . 3 Wider G. . . 4 Senn H. . . 5 Wuthrich K. . . stop_ _Journal_abbreviation 'Proc. Natl. Acad. Sci. U.S.A.' _Journal_name_full . _Journal_volume 95 _Journal_issue . _Journal_CSD . _Book_title . _Book_chapter_title . _Book_volume . _Book_series . _Book_publisher . _Book_publisher_city . _Book_ISBN . _Conference_title . _Conference_site . _Conference_state_province . _Conference_country . _Conference_start_date . _Conference_end_date . _Conference_abstract_number . _Thesis_institution . _Thesis_institution_city . _Thesis_institution_country . _Page_first 13585 _Page_last 13590 _Year 1998 _Details . loop_ _Keyword '1JCACO measurement' '1JCAHA measurement' 'TROSY method' stop_ save_ save_Lohr_et_al_1999 _Saveframe_category citation _Citation_full . _Citation_title 'Recording heteronuclear quantitative J-correlation spectra with internal reference peaks' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Lohr F. . . 2 Perez C. . . 3 Schmidt J. M. . 4 Ruterjans H. . . stop_ _Journal_abbreviation 'Bull. Magn. Reson.' _Journal_name_full . _Journal_volume 20 _Journal_issue . _Journal_CSD . _Book_title . _Book_chapter_title . _Book_volume . _Book_series . _Book_publisher . _Book_publisher_city . _Book_ISBN . _Conference_title . _Conference_site . _Conference_state_province . _Conference_country . _Conference_start_date . _Conference_end_date . _Conference_abstract_number . _Thesis_institution . _Thesis_institution_city . _Thesis_institution_country . _Page_first 9 _Page_last 14 _Year 1999 _Details . loop_ _Keyword '1JCAN measurement' 'quantitative J-correlation' stop_ save_ save_Permi_et_al_1999 _Saveframe_category citation _Citation_full . _Citation_title 'HN(a/b-COCA-J) experiment for measurement of 1JCOCa couplings from Two-Dimensional [15N,1H] Correlation Spectrum' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 10527742 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Permi P. . . 2 Sorsa T. . . 3 Kilpelainen I. . . 4 Annila A. . . stop_ _Journal_abbreviation 'J. Magn. Reson.' _Journal_name_full . _Journal_volume 141 _Journal_issue . _Journal_CSD . _Book_title . _Book_chapter_title . _Book_volume . _Book_series . _Book_publisher . _Book_publisher_city . _Book_ISBN . _Conference_title . _Conference_site . _Conference_state_province . _Conference_country . _Conference_start_date . _Conference_end_date . _Conference_abstract_number . _Thesis_institution . _Thesis_institution_city . _Thesis_institution_country . _Page_first 44 _Page_last 51 _Year 1999 _Details . loop_ _Keyword '1JCACO measurement' stop_ save_ save_Salzmann_et_al_1999 _Saveframe_category citation _Citation_full . _Citation_title 'TROSY-type triple-resonance experiments for sequential NMR assignments of large proteins' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Salzmann M. . . 2 Wider G. . . 3 Pervushin K. . . 4 Senn H. . . 5 Wuthrich K. . . stop_ _Journal_abbreviation 'J. Am. Chem. Soc.' _Journal_name_full . _Journal_volume 121 _Journal_issue . _Journal_CSD . _Book_title . _Book_chapter_title . _Book_volume . _Book_series . _Book_publisher . _Book_publisher_city . _Book_ISBN . _Conference_title . _Conference_site . _Conference_state_province . _Conference_country . _Conference_start_date . _Conference_end_date . _Conference_abstract_number . _Thesis_institution . _Thesis_institution_city . _Thesis_institution_country . _Page_first 844 _Page_last 848 _Year 1999 _Details . loop_ _Keyword '1JCACB measurement' 'TROSY method' stop_ save_ save_Yang_et_al_1999 _Saveframe_category citation _Citation_full . _Citation_title 'TROSY-based HNCO pulse sequence for the measurement of 1HN-15N, 15N-13CO, 1HN-13CO, 13CO-13Ca and 1HN-13Ca dipolar couplings in 15N, 13C, 2H-labeled proteins' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Yang D. . . 2 Venters R. A. . 3 Mueller G. A. . 4 Choy W. Y. . 5 Kay L. E. . stop_ _Journal_abbreviation 'J. Biomol. NMR' _Journal_name_full . _Journal_volume 14 _Journal_issue . _Journal_CSD . _Book_title . _Book_chapter_title . _Book_volume . _Book_series . _Book_publisher . _Book_publisher_city . _Book_ISBN . _Conference_title . _Conference_site . _Conference_state_province . _Conference_country . _Conference_start_date . _Conference_end_date . _Conference_abstract_number . _Thesis_institution . _Thesis_institution_city . _Thesis_institution_country . _Page_first 333 _Page_last 343 _Year 1999 _Details . loop_ _Keyword '1JCACO measurement' stop_ save_ save_Permi_&_Annila_2000 _Saveframe_category citation _Citation_full . _Citation_title 'Transverse relaxation optimised spin-state selective NMR experiments for measurement of residual dipolar couplings' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 10805128 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Permi P. . . 2 Annila A. . . stop_ _Journal_abbreviation 'J. Biomol. NMR' _Journal_name_full . _Journal_volume 16 _Journal_issue . _Journal_CSD . _Book_title . _Book_chapter_title . _Book_volume . _Book_series . _Book_publisher . _Book_publisher_city . _Book_ISBN . _Conference_title . _Conference_site . _Conference_state_province . _Conference_country . _Conference_start_date . _Conference_end_date . _Conference_abstract_number . _Thesis_institution . _Thesis_institution_city . _Thesis_institution_country . _Page_first 221 _Page_last 227 _Year 2000 _Details . loop_ _Keyword '1JCAN measurement' stop_ save_ save_Evenas_et_al_2001 _Saveframe_category citation _Citation_full . _Citation_title 'Measurement of 13Ca-13Cb dipolar couplings in 15N,13C,2H-labeled proteins: Application to domain orientation in maltose binding protein' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 11456973 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Evenas J. . . 2 Mittermaier A. . . 3 Yang D. . . 4 Kay L. E. . stop_ _Journal_abbreviation 'J. Am. Chem. Soc.' _Journal_name_full . _Journal_volume 123 _Journal_issue . _Journal_CSD . _Book_title . _Book_chapter_title . _Book_volume . _Book_series . _Book_publisher . _Book_publisher_city . _Book_ISBN . _Conference_title . _Conference_site . _Conference_state_province . _Conference_country . _Conference_start_date . _Conference_end_date . _Conference_abstract_number . _Thesis_institution . _Thesis_institution_city . _Thesis_institution_country . _Page_first 2858 _Page_last 2864 _Year 2001 _Details . loop_ _Keyword '1JCACB measurement' stop_ save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name Ubiquitin _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label protein $Ubiquitin stop_ _System_molecular_weight 8565.2 _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . loop_ _Biological_function 'protein degradation' stop_ _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_Ubiquitin _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common Ubiquitin _Molecular_mass 8565.2 _Mol_thiol_state 'not present' loop_ _Biological_function 'protein degradation' stop_ _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 76 _Mol_residue_sequence ; MQIFVKTLTGKTITLEVEPS DTIENVKAKIQDKEGIPPDQ QRLIFAGKQLEDGRTLSDYN IQKESTLHLVLRLRGG ; loop_ _Residue_seq_code _Residue_label 1 MET 2 GLN 3 ILE 4 PHE 5 VAL 6 LYS 7 THR 8 LEU 9 THR 10 GLY 11 LYS 12 THR 13 ILE 14 THR 15 LEU 16 GLU 17 VAL 18 GLU 19 PRO 20 SER 21 ASP 22 THR 23 ILE 24 GLU 25 ASN 26 VAL 27 LYS 28 ALA 29 LYS 30 ILE 31 GLN 32 ASP 33 LYS 34 GLU 35 GLY 36 ILE 37 PRO 38 PRO 39 ASP 40 GLN 41 GLN 42 ARG 43 LEU 44 ILE 45 PHE 46 ALA 47 GLY 48 LYS 49 GLN 50 LEU 51 GLU 52 ASP 53 GLY 54 ARG 55 THR 56 LEU 57 SER 58 ASP 59 TYR 60 ASN 61 ILE 62 GLN 63 LYS 64 GLU 65 SER 66 THR 67 LEU 68 HIS 69 LEU 70 VAL 71 LEU 72 ARG 73 LEU 74 ARG 75 GLY 76 GLY stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-11-18 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 11505 entity 100.00 76 98.68 98.68 3.54e-45 BMRB 11547 ubiquitin 100.00 76 98.68 98.68 3.54e-45 BMRB 15047 denatured_ubiquitin 100.00 76 100.00 100.00 6.54e-46 BMRB 15410 Ubi 100.00 76 100.00 100.00 6.54e-46 BMRB 15689 UBB 98.68 103 98.67 100.00 6.21e-44 BMRB 16228 ubiquitin 100.00 76 97.37 98.68 1.73e-44 BMRB 16582 Ubiquitin 100.00 76 100.00 100.00 6.54e-46 BMRB 16626 Ubiquitin 100.00 76 100.00 100.00 6.54e-46 BMRB 16895 UBB+1 98.68 103 98.67 100.00 6.21e-44 BMRB 17181 ubiquitin 100.00 76 100.00 100.00 6.54e-46 BMRB 17439 ubiquitin 100.00 76 100.00 100.00 6.54e-46 BMRB 17769 Ubiquitin 100.00 76 100.00 100.00 6.54e-46 BMRB 17919 entity 100.00 76 100.00 100.00 6.54e-46 BMRB 18582 ubiquitin 100.00 76 100.00 100.00 6.54e-46 BMRB 18583 ubiquitin 100.00 76 100.00 100.00 6.54e-46 BMRB 18584 ubiquitin 100.00 76 100.00 100.00 6.54e-46 BMRB 18610 Ubiquitin 100.00 76 100.00 100.00 6.54e-46 BMRB 18611 Ubiquitin_A_state 100.00 76 100.00 100.00 6.54e-46 BMRB 18737 UBIQUITIN 100.00 76 100.00 100.00 6.54e-46 BMRB 19406 entity 100.00 152 100.00 100.00 6.43e-45 BMRB 19412 entity 100.00 152 100.00 100.00 6.43e-45 BMRB 25070 Ubiquitin 100.00 79 100.00 100.00 6.66e-46 BMRB 25123 Ubiquitin 94.74 72 100.00 100.00 8.52e-43 BMRB 25601 entity_1 100.00 76 97.37 97.37 5.31e-44 BMRB 26604 Ubiquitin_(microcrystalline) 100.00 76 100.00 100.00 6.54e-46 BMRB 4245 ubiquitin 100.00 76 100.00 100.00 6.54e-46 BMRB 4375 Ubiquitin 100.00 76 100.00 100.00 6.54e-46 PDB 1AAR "Structure Of A Diubiquitin Conjugate And A Model For Interaction With Ubiquitin Conjugating Enzyme (E2)" 100.00 76 100.00 100.00 6.54e-46 PDB 1CMX "Structural Basis For The Specificity Of Ubiquitin C- Terminal Hydrolases" 98.68 76 100.00 100.00 4.40e-45 PDB 1D3Z "Ubiquitin Nmr Structure" 100.00 76 100.00 100.00 6.54e-46 PDB 1F9J "Structure Of A New Crystal Form Of Tetraubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 1FXT "Structure Of A Conjugating Enzyme-Ubiquitin Thiolester Complex" 100.00 76 100.00 100.00 6.54e-46 PDB 1G6J "Structure Of Recombinant Human Ubiquitin In Aot Reverse Micelles" 98.68 76 100.00 100.00 7.58e-45 PDB 1GJZ "Solution Structure Of A Dimeric N-Terminal Fragment Of Human Ubiquitin" 67.11 53 100.00 100.00 3.14e-26 PDB 1NBF "Crystal Structure Of A Ubp-Family Deubiquitinating Enzyme In Isolation And In Complex With Ubiquitin Aldehyde" 98.68 76 100.00 100.00 4.40e-45 PDB 1OGW "Synthetic Ubiquitin With Fluoro-Leu At 50 And 67" 100.00 76 97.37 97.37 2.65e-44 PDB 1P3Q "Mechanism Of Ubiquitin Recognition By The Cue Domain Of Vps9" 100.00 76 100.00 100.00 6.54e-46 PDB 1Q5W "Ubiquitin Recognition By Npl4 Zinc-Fingers" 100.00 76 100.00 100.00 6.54e-46 PDB 1S1Q "Tsg101(Uev) Domain In Complex With Ubiquitin" 98.68 76 100.00 100.00 7.58e-45 PDB 1TBE "Structure Of Tetraubiquitin Shows How Multiubiquitin Chains Can Be Formed" 100.00 76 100.00 100.00 6.54e-46 PDB 1UBI "Synthetic Structural And Biological Studies Of The Ubiquitin System. Part 1" 100.00 76 100.00 100.00 6.54e-46 PDB 1UBQ "Structure Of Ubiquitin Refined At 1.8 Angstroms Resolution" 100.00 76 100.00 100.00 6.54e-46 PDB 1UZX "A Complex Of The Vps23 Uev With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 1V80 "Solution Structures Of Ubiquitin At 30 Bar And 3 Kbar" 100.00 76 100.00 100.00 6.54e-46 PDB 1V81 "Solution Structures Of Ubiquitin At 30 Bar And 3 Kbar" 100.00 76 100.00 100.00 6.54e-46 PDB 1VX7 "Cryo-em Structure Of The Plasmodium Falciparum 80s Ribosome Bound To The Anti-protozoan Drug Emetine, Large Subunit (protein On" 100.00 128 98.68 100.00 1.38e-45 PDB 1WR6 "Crystal Structure Of Gga3 Gat Domain In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 1WRD "Crystal Structure Of Tom1 Gat Domain In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 1XD3 "Crystal Structure Of Uchl3-Ubvme Complex" 98.68 75 100.00 100.00 4.28e-45 PDB 1XQQ "Simultaneous Determination Of Protein Structure And Dynamics" 100.00 76 100.00 100.00 6.54e-46 PDB 1YD8 "Complex Of Human Gga3 Gat Domain And Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 1YIW "X-Ray Crystal Structure Of A Chemically Synthesized Ubiquitin" 100.00 76 98.68 100.00 2.84e-45 PDB 1YJ1 "X-Ray Crystal Structure Of A Chemically Synthesized [d-Gln35]ubiquitin" 100.00 76 97.37 98.68 3.36e-44 PDB 1YX5 "Solution Structure Of S5a Uim-1UBIQUITIN COMPLEX" 100.00 98 100.00 100.00 2.31e-46 PDB 1YX6 "Solution Structure Of S5a Uim-2UBIQUITIN COMPLEX" 100.00 98 100.00 100.00 2.31e-46 PDB 1ZGU "Solution Structure Of The Human Mms2-Ubiquitin Complex" 100.00 76 98.68 100.00 1.56e-45 PDB 2AYO "Structure Of Usp14 Bound To Ubquitin Aldehyde" 98.68 76 100.00 100.00 4.40e-45 PDB 2BGF "Nmr Structure Of Lys48-Linked Di-Ubiquitin Using Chemical Shift Perturbation Data Together With Rdcs And 15n- Relaxation Data" 100.00 76 100.00 100.00 6.54e-46 PDB 2C7M "Human Rabex-5 Residues 1-74 In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2C7N "Human Rabex-5 Residues 1-74 In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2D3G "Double Sided Ubiquitin Binding Of Hrs-Uim" 100.00 76 100.00 100.00 6.54e-46 PDB 2DEN "Solution Structure Of The Ubiquitin-Associated Domain Of Human Bmsc-Ubp And Its Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2DX5 "The Complex Structure Between The Mouse Eap45-Glue Domain And Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2FCM "X-Ray Crystal Structure Of A Chemically Synthesized [d-Gln35]ubiquitin With A Cubic Space Group" 100.00 76 97.37 98.68 3.36e-44 PDB 2FCN "X-Ray Crystal Structure Of A Chemically Synthesized [d-Val35]ubiquitin With A Cubic Space Group" 100.00 76 97.37 98.68 3.36e-44 PDB 2FCQ "X-Ray Crystal Structure Of A Chemically Synthesized Ubiquitin With A Cubic Space Group" 100.00 76 98.68 100.00 2.84e-45 PDB 2FCS "X-Ray Crystal Structure Of A Chemically Synthesized [l-Gln35]ubiquitin With A Cubic Space Group" 100.00 76 97.37 98.68 3.83e-44 PDB 2FID "Crystal Structure Of A Bovine Rabex-5 Fragment Complexed With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2FIF "Crystal Structure Of A Bovine Rabex-5 Fragment Complexed With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2FUH "Solution Structure Of The Ubch5cUB NON-Covalent Complex" 100.00 76 100.00 100.00 6.54e-46 PDB 2G45 "Co-Crystal Structure Of Znf Ubp Domain From The Deubiquitinating Enzyme Isopeptidase T (Isot) In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2GMI Mms2UBC13~UBIQUITIN 100.00 76 100.00 100.00 6.54e-46 PDB 2HD5 "Usp2 In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2HTH "Structural Basis For Ubiquitin Recognition By The Human Eap45ESCRT-Ii Glue Domain" 100.00 76 100.00 100.00 6.54e-46 PDB 2IBI "Covalent Ubiquitin-Usp2 Complex" 98.68 75 100.00 100.00 4.28e-45 PDB 2J7Q "Crystal Structure Of The Ubiquitin-Specific Protease Encoded By Murine Cytomegalovirus Tegument Protein M48 In Complex With A U" 98.68 75 100.00 100.00 4.28e-45 PDB 2JF5 "Crystal Structure Of Lys63-Linked Di-Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2JRI "Solution Structure Of The Josephin Domain Of Ataxin-3 In Complex With Ubiquitin Molecule." 100.00 76 100.00 100.00 6.54e-46 PDB 2JY6 "Solution Structure Of The Complex Of Ubiquitin And Ubiquilin 1 Uba Domain" 100.00 76 100.00 100.00 6.54e-46 PDB 2JZZ "Solid-State Nmr Structure Of Microcrystalline Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2K25 "Automated Nmr Structure Of The Ubb By Fapsy" 98.68 103 98.67 100.00 6.21e-44 PDB 2K39 "Recognition Dynamics Up To Microseconds Revealed From Rdc Derived Ubiquitin Ensemble In Solution" 100.00 76 100.00 100.00 6.54e-46 PDB 2K6D "Cin85 Sh3-C Domain In Complex With Ubiquitin" 98.68 76 100.00 100.00 3.99e-45 PDB 2K8B "Solution Structure Of Plaa Family Ubiquitin Binding Domain (Pfuc) Cis Isomer In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2K8C "Solution Structure Of Plaa Family Ubiquitin Binding Domain (Pfuc) Trans Isomer In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2KDE "Nmr Structure Of Major S5a (196-306):k48 Linked Diubiquitin Species" 100.00 76 100.00 100.00 6.54e-46 PDB 2KDF "Nmr Structure Of Minor S5a (196-306):k48 Linked Diubiquitin Species" 100.00 76 100.00 100.00 6.54e-46 PDB 2KHW "Solution Structure Of The Human Polymerase Iota Ubm2- Ubiquitin Complex" 100.00 79 100.00 100.00 6.66e-46 PDB 2KJH "Nmr Based Structural Model Of The Ubch8-Ubiquitin Complex" 98.68 76 100.00 100.00 3.99e-45 PDB 2KLG "Pere Nmr Structure Of Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2KN5 "A Correspondence Between Solution-State Dynamics Of An Individual Protein And The Sequence And Conformational Diversity Of Its " 100.00 76 100.00 100.00 6.54e-46 PDB 2KOX "Nmr Residual Dipolar Couplings Identify Long Range Correlated Motions In The Backbone Of The Protein Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2KTF "Solution Nmr Structure Of Human Polymerase Iota Ubm2 In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2KWU "Solution Structure Of Ubm2 Of Murine Polymerase Iota In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2KWV "Solution Structure Of Ubm1 Of Murine Polymerase Iota In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2KX0 "The Solution Structure Of Ubb+1, Frameshift Mutant Of Ubiquitin B" 98.68 103 98.67 100.00 6.21e-44 PDB 2L0F "Solution Nmr Structure Of Human Polymerase Iota Ubm2 (P692a Mutant) In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2L0T "Solution Structure Of The Complex Of Ubiquitin And The Vhs Domain Of Stam2" 100.00 76 100.00 100.00 6.54e-46 PDB 2L3Z "Proton-Detected 4d Dream Solid-State Nmr Structure Of Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2LD9 "Backbone Structure Of Ubiquitin Determined Using Backbone Amide Noes And Backbone N-H And N-C Rdcs" 100.00 77 100.00 100.00 7.92e-46 PDB 2LJ5 "Description Of The Structural Fluctuations Of Proteins From Structure- Based Calculations Of Residual Dipolar Couplings" 100.00 76 100.00 100.00 6.54e-46 PDB 2LVO "Structure Of The Gp78cue Domain Bound To Monubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2LVP "Gp78cue Domain Bound To The Distal Ubiquitin Of K48-Linked Diubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2LVQ "Gp78cue Domain Bound To The Proximal Ubiquitin Of K48-Linked Diubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2LZ6 "Distinct Ubiquitin Binding Modes Exhibited By Sh3 Domains: Molecular Determinants And Functional Implications" 100.00 76 100.00 100.00 6.54e-46 PDB 2MBB "Solution Structure Of The Human Polymerase Iota Ubm1-ubiquitin Complex" 100.00 78 100.00 100.00 5.38e-46 PDB 2MBH "Nmr Structure Of Eklf(22-40)/ubiquitin Complex" 100.00 76 100.00 100.00 6.54e-46 PDB 2MBO "K11-linked Diubiquitin Average Solution Structure At Ph 6.8, 0 Mm Nacl" 100.00 76 100.00 100.00 6.54e-46 PDB 2MBQ "K11-linked Diubiquitin Average Solution Structure At Ph 6.8, 150 Mm Nacl" 100.00 76 100.00 100.00 6.54e-46 PDB 2MCN "Distinct Ubiquitin Binding Modes Exhibited By Sh3 Domains: Molecular Determinants And Functional Implications" 100.00 76 100.00 100.00 6.54e-46 PDB 2MJ5 "Structure Of The Uba Domain Of Human Nbr1 In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2MJB "Solution Nmr Structure Of Ubiquitin Refined Against Dipolar Couplings In 4 Media" 100.00 76 100.00 100.00 6.54e-46 PDB 2MOR "A Tensor-free Method For The Structural And Dynamical Refinement Of Proteins Using Residual Dipolar Couplings" 100.00 76 100.00 100.00 6.54e-46 PDB 2MRE "Nmr Structure Of The Rad18-ubz/ubiquitin Complex" 100.00 79 100.00 100.00 6.66e-46 PDB 2MRO "Structure Of The Complex Of Ubiquitin And The Uba Domain From Dna- Damage-inducible 1 Protein (ddi1)" 100.00 76 100.00 100.00 6.54e-46 PDB 2MSG "Solid-state Nmr Structure Of Ubiquitin" 94.74 72 100.00 100.00 8.52e-43 PDB 2MUR "Solution Structure Of The Human Faap20 Ubz-ubiquitin Complex" 100.00 78 100.00 100.00 5.38e-46 PDB 2MWS "Structure Of The Complex Of Ubiquitin And The Ubiquitin-like (ubl) Domain Of Ddi1" 100.00 76 98.68 98.68 4.40e-45 PDB 2N2K "Ensemble Structure Of The Closed State Of Lys63-linked Diubiquitin In The Absence Of A Ligand" 93.42 71 100.00 100.00 6.21e-42 PDB 2NR2 "The Mumo (Minimal Under-Restraining Minimal Over- Restraining) Method For The Determination Of Native States Ensembles Of Prote" 100.00 76 100.00 100.00 6.54e-46 PDB 2O6V "Crystal Structure And Solution Nmr Studies Of Lys48-Linked Tetraubiquitin At Neutral Ph" 100.00 76 100.00 100.00 6.54e-46 PDB 2OJR "Structure Of Ubiquitin Solved By Sad Using The Lanthanide- Binding Tag" 100.00 111 100.00 100.00 2.29e-45 PDB 2OOB "Crystal Structure Of The Uba Domain From Cbl-B Ubiquitin Ligase In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2PE9 "Nmr Based Structure Of The Open Conformation Of Lys48- Linked Di-Ubiquitin Using Experimental Global Rotational Diffusion Tenso" 100.00 76 100.00 100.00 6.54e-46 PDB 2PEA "Nmr Based Structure Of The Closed Conformation Of Lys48- Linked Di-Ubiquitin Using Experimental Global Rotational Diffusion Ten" 100.00 76 100.00 100.00 6.54e-46 PDB 2QHO "Crystal Structure Of The Uba Domain From Edd Ubiquitin Ligase In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2RR9 "The Solution Structure Of The K63-Ub2:tuims Complex" 100.00 76 100.00 100.00 6.54e-46 PDB 2RSU "Alternative Structure Of Ubiquitin" 100.00 76 98.68 98.68 3.54e-45 PDB 2RU6 "The Pure Alternative State Of Ubiquitin" 100.00 76 98.68 98.68 3.54e-45 PDB 2W9N "Crystal Structure Of Linear Di-Ubiquitin" 98.68 152 100.00 100.00 5.62e-44 PDB 2WDT "Crystal Structure Of Plasmodium Falciparum Uchl3 In Complex With The Suicide Inhibitor Ubvme" 98.68 76 100.00 100.00 4.40e-45 PDB 2WWZ "Tab2 Nzf Domain In Complex With Lys63-Linked Di-Ubiquitin, P212121" 100.00 76 100.00 100.00 6.54e-46 PDB 2WX0 "Tab2 Nzf Domain In Complex With Lys63-Linked Di-Ubiquitin, P21" 100.00 76 100.00 100.00 6.54e-46 PDB 2WX1 "Tab2 Nzf Domain In Complex With Lys63-Linked Tri-Ubiquitin, P212121" 100.00 76 100.00 100.00 6.54e-46 PDB 2XBB "Nedd4 Hect:ub Complex" 100.00 76 100.00 100.00 6.54e-46 PDB 2XEW "Crystal Structure Of K11-Linked Diubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2XK5 "Crystal Structure Of K6-Linked Diubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2Y5B "Structure Of Usp21 In Complex With Linear Diubiquitin-Aldehyde" 98.68 152 100.00 100.00 4.15e-44 PDB 2Z59 "Complex Structures Of Mouse Rpn13 (22-130aa) And Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2ZCB "Crystal Structure Of Ubiquitin P37aP38A" 100.00 76 97.37 97.37 3.44e-44 PDB 2ZCC "Ubiquitin Crystallized Under High Pressure" 100.00 76 100.00 100.00 6.54e-46 PDB 2ZNV "Crystal Structure Of Human Amsh-Lp Dub Domain In Complex With Lys63-Linked Ubiquitin Dimer" 100.00 77 100.00 100.00 7.42e-46 PDB 2ZVN "Nemo Cozi Domain Incomplex With Diubiquitin In P212121 Space Group" 100.00 154 100.00 100.00 5.98e-45 PDB 2ZVO "Nemo Cozi Domain In Complex With Diubiquitin In C2 Space Group" 100.00 154 100.00 100.00 5.98e-45 PDB 3A1Q "Crystal Structure Of The Mouse Rap80 Uims In Complex With Lys63-Linked Di-Ubiquitin" 100.00 77 100.00 100.00 7.42e-46 PDB 3A33 "Ubch5b~ubiquitin Conjugate" 100.00 76 100.00 100.00 6.54e-46 PDB 3A9J "Crystal Structure Of The Mouse Tab2-Nzf In Complex With Lys63-Linked Di-Ubiquitin" 100.00 77 100.00 100.00 7.42e-46 PDB 3A9K "Crystal Structure Of The Mouse Tab3-Nzf In Complex With Lys63-Linked Di-Ubiquitin" 100.00 77 100.00 100.00 7.42e-46 PDB 3AI5 "Crystal Structure Of Yeast Enhanced Green Fluorescent Protein- Ubiquitin Fusion Protein" 97.37 307 100.00 100.00 1.64e-41 PDB 3ALB "Cyclic Lys48-Linked Tetraubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 3AUL "Crystal Structure Of Wild-Type Lys48-Linked Diubiquitin In An Open Conformation" 100.00 76 100.00 100.00 6.54e-46 PDB 3AXC "Crystal Structure Of Linear Diubiquitin" 100.00 154 100.00 100.00 5.98e-45 PDB 3B08 "Crystal Structure Of The Mouse Hoil1-l-nzf In Complex With Linear Di- Ubiquitin" 100.00 152 100.00 100.00 6.43e-45 PDB 3B0A "Crystal Structure Of The Mouse Hoil1-l-nzf In Complex With Linear Di- Ubiquitin" 100.00 152 100.00 100.00 6.43e-45 PDB 3BY4 "Structure Of Ovarian Tumor (Otu) Domain In Complex With Ubiquitin" 98.68 75 100.00 100.00 4.28e-45 PDB 3C0R "Structure Of Ovarian Tumor (Otu) Domain In Complex With Ubiquitin" 98.68 75 100.00 100.00 4.28e-45 PDB 3DVG "Crystal Structure Of K63-Specific Fab Apu.3a8 Bound To K63-Linked Di- Ubiquitin" 100.00 80 100.00 100.00 8.24e-46 PDB 3DVN "Crystal Structure Of K63-specific Fab Apu2.16 Bound To K63-linked Di- Ubiquitin" 100.00 80 100.00 100.00 8.24e-46 PDB 3EEC "X-Ray Structure Of Human Ubiquitin Cd(Ii) Adduct" 100.00 76 100.00 100.00 6.54e-46 PDB 3EFU "X-Ray Structure Of Human Ubiquitin-Hg(Ii) Adduct" 100.00 76 100.00 100.00 6.54e-46 PDB 3EHV "X-Ray Structure Of Human Ubiquitin Zn(Ii) Adduct" 100.00 76 100.00 100.00 6.54e-46 PDB 3H1U "Structure Of Ubiquitin In Complex With Cd Ions" 100.00 76 100.00 100.00 6.54e-46 PDB 3H7P "Crystal Structure Of K63-Linked Di-Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 3H7S "Crystal Structures Of K63-Linked Di- And Tri-Ubiquitin Reveal A Highly Extended Chain Architecture" 100.00 76 98.68 98.68 2.81e-43 PDB 3HM3 "The Structure And Conformation Of Lys-63 Linked Tetra-Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 3I3T "Crystal Structure Of Covalent Ubiquitin-usp21 Complex" 98.68 75 100.00 100.00 4.28e-45 PDB 3IFW "Crystal Structure Of The S18y Variant Of Ubiquitin Carboxy T Hydrolase L1 Bound To Ubiquitin Vinylmethylester." 98.68 75 100.00 100.00 4.28e-45 PDB 3IHP "Covalent Ubiquitin-Usp5 Complex" 98.68 75 100.00 100.00 4.28e-45 PDB 3JSV "Crystal Structure Of Mouse Nemo Cozi In Complex With Lys63- Linked Di-Ubiquitin" 100.00 77 100.00 100.00 7.42e-46 PDB 3JVZ E2~ubiquitin-Hect 100.00 81 100.00 100.00 7.68e-46 PDB 3JW0 E2~ubiquitin-Hect 100.00 81 100.00 100.00 7.68e-46 PDB 3K9O "The Crystal Structure Of E2-25k And Ubb+1 Complex" 98.68 96 100.00 100.00 1.27e-44 PDB 3K9P "The Crystal Structure Of E2-25k And Ubiquitin Complex" 100.00 79 100.00 100.00 6.66e-46 PDB 3KVF "Crystal Structure Of The I93m Mutant Of Ubiquitin Carboxy Te Hydrolase L1 Bound To Ubiquitin Vinylmethylester" 98.68 75 100.00 100.00 4.28e-45 PDB 3KW5 "Crystal Structure Of Ubiquitin Carboxy Terminal Hydrolase L1 Ubiquitin Vinylmethylester" 98.68 75 100.00 100.00 4.28e-45 PDB 3LDZ "Crystal Structure Of Human Stam1 Vhs Domain In Complex With Ubiquitin" 96.05 73 100.00 100.00 1.79e-43 PDB 3M3J "A New Crystal Form Of Lys48-Linked Diubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 3MHS "Structure Of The Saga Ubp8SGF11SUS1SGF73 DUB MODULE BOUND Ubiquitin Aldehyde" 98.68 76 100.00 100.00 4.40e-45 PDB 3MTN "Usp21 In Complex With A Ubiquitin-based, Usp21-specific Inhibitor" 88.16 85 98.51 98.51 1.47e-37 PDB 3N30 "Crystal Structure Of Cubic Zn3-Hub (Human Ubiquitin) Adduct" 100.00 76 100.00 100.00 6.54e-46 PDB 3N32 "The Crystal Structure Of Human Ubiquitin Adduct With Zeise's Salt" 100.00 76 100.00 100.00 6.54e-46 PDB 3NHE "High Resolution Structure (1.26a) Of Usp2a In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 3NOB "Structure Of K11-linked Di-ubiquitin" 100.00 78 100.00 100.00 5.38e-46 PDB 3NS8 "Crystal Structure Of An Open Conformation Of Lys48-Linked Diubiquitin At Ph 7.5" 100.00 76 100.00 100.00 6.54e-46 PDB 3O65 "Crystal Structure Of A Josephin-Ubiquitin Complex: Evolutionary Restraints On Ataxin-3 Deubiquitinating Activity" 98.68 76 100.00 100.00 4.40e-45 PDB 3OFI "Crystal Structure Of Human Insulin-Degrading Enzyme In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 3OJ3 "Crystal Structure Of The A20 Znf4 And Ubiquitin Complex" 100.00 79 100.00 100.00 6.66e-46 PDB 3OJ4 "Crystal Structure Of The A20 Znf4, Ubiquitin And Ubch5a Complex" 100.00 79 100.00 100.00 6.66e-46 PDB 3ONS "Crystal Structure Of Human Ubiquitin In A New Crystal Form" 94.74 72 100.00 100.00 8.52e-43 PDB 3PHD "Crystal Structure Of Human Hdac6 In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 3PHW "Otu Domain Of Crimean Congo Hemorrhagic Fever Virus In Complex With Ubiquitin" 98.68 75 100.00 100.00 4.28e-45 PDB 3PRM "Structural Analysis Of A Viral Otu Domain Protease From The Crimean- Congo Hemorrhagic Fever Virus In Complex With Human Ubiqui" 98.68 75 100.00 100.00 4.28e-45 PDB 3PRP "Structural Analysis Of A Viral Otu Domain Protease From The Crimean- Congo Hemorrhagic Fever Virus In Complex With Human Ubiqui" 98.68 75 100.00 100.00 4.28e-45 PDB 3PT2 "Structure Of A Viral Otu Domain Protease Bound To Ubiquitin" 98.68 75 100.00 100.00 4.28e-45 PDB 3PTF "X-Ray Structure Of The Non-Covalent Complex Between Ubch5a And Ubiquitin" 100.00 79 100.00 100.00 6.66e-46 PDB 3Q3F "Engineering Domain-Swapped Binding Interfaces By Mutually Exclusive Folding: Insertion Of Ubiquitin Into Position 103 Of Barnas" 98.68 189 100.00 100.00 5.35e-44 PDB 3RUL "New Strategy To Analyze Structures Of Glycopeptide-Target Complexes" 98.68 79 100.00 100.00 3.97e-45 PDB 3TBL "Structure Of Mono-ubiquitinated Pcna: Implications For Dna Polymerase Switching And Okazaki Fragment Maturation" 100.00 76 100.00 100.00 6.54e-46 PDB 3TMP "The Catalytic Domain Of Human Deubiquitinase Duba In Complex With Ubiquitin Aldehyde" 98.68 76 100.00 100.00 4.40e-45 PDB 3U30 "Crystal Structure Of A Linear-Specific Ubiquitin Fab Bound To Linear Ubiquitin" 100.00 172 100.00 100.00 6.89e-45 PDB 3UGB "Ubch5c~ubiquitin Conjugate" 100.00 76 100.00 100.00 6.54e-46 PDB 3VDZ "Tailoring Encodable Lanthanide-Binding Tags As Mri Contrast Agents: Xq-Dse3-Ubiquitin At 2.4 Angstroms" 100.00 111 100.00 100.00 1.47e-45 PDB 3VFK "The Structure Of Monodechloro-teicoplanin In Complex With Its Ligand, Using Ubiquitin As A Ligand Carrier" 98.68 79 100.00 100.00 3.97e-45 PDB 3VHT "Crystal Structure Of Gfp-Wrnip1 Ubz Domain Fusion Protein In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 3VUW "Crystal Structure Of A20 Zf7 In Complex With Linear Ubiquitin, Form I" 100.00 76 100.00 100.00 6.54e-46 PDB 3VUX "Crystal Structure Of A20 Zf7 In Complex With Linear Ubiquitin, Form Ii" 100.00 76 100.00 100.00 6.54e-46 PDB 3VUY "Crystal Structure Of A20 Zf7 In Complex With Linear Tetraubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 3WWQ "Crystal Structure Of Faap20 Ubz Domain In Complex With Lys63-linked Diubiquitin" 100.00 77 100.00 100.00 7.42e-46 PDB 3WXE "Crystal Structure Of Cyld Usp Domain (c596s) In Complex With Met1- Linked Diubiquitin" 94.74 148 100.00 100.00 9.19e-42 PDB 3WXF "Crystal Structure Of Cyld Usp Domain (c596s E674q) In Complex With Met1-linked Diubiquitin" 94.74 148 100.00 100.00 9.19e-42 PDB 3WXG "Crystal Structure Of Cyld Usp Domain (c596a) In Complex With Lys63- Linked Diubiquitin" 94.74 72 100.00 100.00 8.52e-43 PDB 3ZLZ "Lys6-linked Tri-ubiquitin" 100.00 76 98.68 100.00 1.56e-45 PDB 3ZNH "Crimean Congo Hemorrhagic Fever Virus Otu Domain In Complex With Ubiquitin-propargyl." 98.68 76 100.00 100.00 4.40e-45 PDB 3ZNI "Structure Of Phosphotyr363-cbl-b - Ubch5b-ub - Zap-70 Peptide Complex" 100.00 81 100.00 100.00 7.68e-46 PDB 3ZNZ "Crystal Structure Of Otulin Otu Domain (c129a) In Complex With Met1-di Ubiquitin" 100.00 152 100.00 100.00 6.43e-45 PDB 4A18 "T.Thermophila 60s Ribosomal Subunit In Complex With Initiation Factor 6. This File Contains 26s Rrna And Proteins Of Molecule 1" 100.00 129 97.37 98.68 2.69e-44 PDB 4A19 "T.Thermophila 60s Ribosomal Subunit In Complex With Initiation Factor 6. This File Contains 26s Rrna And Proteins Of Molecule 2" 100.00 129 97.37 98.68 2.69e-44 PDB 4A1B "T.Thermophila 60s Ribosomal Subunit In Complex With Initiation Factor 6. This File Contains 26s Rrna And Proteins Of Molecule 3" 100.00 129 97.37 98.68 2.69e-44 PDB 4A1D "T.Thermophila 60s Ribosomal Subunit In Complex With Initiation Factor 6. This File Contains 26s Rrna And Proteins Of Molecule 4" 100.00 129 97.37 98.68 2.69e-44 PDB 4ADX "The Cryo-em Structure Of The Archaeal 50s Ribosomal Subunit In Complex With Initiation Factor 6" 100.00 129 97.37 98.68 2.69e-44 PDB 4AP4 "Rnf4 - Ubch5a - Ubiquitin Heterotrimeric Complex" 100.00 80 100.00 100.00 6.85e-46 PDB 4AUQ "Structure Of Birc7-Ubch5b-Ub Complex." 100.00 81 100.00 100.00 7.68e-46 PDB 4BBN "Nedd4 Hect-ub:ub Complex" 100.00 76 100.00 100.00 6.54e-46 PDB 4BOS "Structure Of Otud2 Otu Domain In Complex With Ubiquitin K11- Linked Peptide" 100.00 76 100.00 100.00 6.54e-46 PDB 4BOZ "Structure Of Otud2 Otu Domain In Complex With K11-linked Di Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 4BVU "Structure Of Shigella Effector Ospg In Complex With Host Ubch5c-ubiquitin Conjugate" 100.00 76 100.00 100.00 6.54e-46 PDB 4CXC "Regulation Of The Mammalian Elongation Cycle By 40s Subunit Rolling: A Eukaryotic-specific Ribosome Rearrangement" 100.00 156 100.00 100.00 7.65e-46 PDB 4CXD "Regulation Of The Mammalian Elongation Cycle By 40s Subunit Rolling: A Eukaryotic-specific Ribosome Rearrangement" 100.00 128 100.00 100.00 4.55e-46 PDB 4D5L "Cryo-em Structures Of Ribosomal 80s Complexes With Termination Factors And Cricket Paralysis Virus Ires Reveal The Ires In The " 100.00 156 100.00 100.00 7.65e-46 PDB 4D61 "Cryo-em Structures Of Ribosomal 80s Complexes With Termination Factors And Cricket Paralysis Virus Ires Reveal The Ires In The " 100.00 156 100.00 100.00 7.65e-46 PDB 4DDG "Crystal Structure Of Human Otub1UBCH5B~UBUB" 100.00 76 100.00 100.00 6.54e-46 PDB 4DDI "Crystal Structure Of Human Otub1UBCH5B~UBUB" 100.00 76 100.00 100.00 6.54e-46 PDB 4DHJ "The Structure Of A Ceotub1 Ubiquitin Aldehyde Ubc13~ub Complex" 98.68 76 100.00 100.00 3.99e-45 PDB 4DHZ "The Structure Of HCEOTUB1-Ubiquitin Aldehyde-Ubc13~ub" 98.68 76 100.00 100.00 3.99e-45 PDB 4FJV "Crystal Structure Of Human Otubain2 And Ubiquitin Complex" 100.00 86 100.00 100.00 9.86e-46 PDB 4HXD "Diversity Of Ubiquitin And Isg15 Specificity Amongst Nairoviruses Viral Ovarian Tumor Domain Proteases" 98.68 75 100.00 100.00 4.28e-45 PDB 4I6N "Crystal Structure Of Trichinella Spiralis Uch37 Catalytic Domain Bound To Ubiquitin Vinyl Methyl Ester" 97.37 75 100.00 100.00 4.54e-44 PDB 4IG7 "Crystal Structure Of Trichinella Spiralis Uch37 Bound To Ubiquitin Vinyl Methyl Ester" 98.68 75 100.00 100.00 4.28e-45 PDB 4IUM "Equine Arteritis Virus Papain-like Protease 2 (plp2) Covalently Bound To Ubiquitin" 98.68 76 100.00 100.00 4.40e-45 PDB 4JIO "Bro1 V Domain And Ubiquitin" 100.00 76 98.68 98.68 4.85e-45 PDB 4JQW "Crystal Structure Of A Complex Of Nod1 Card And Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 4K1R "Crystal Structure Of Schizosaccharomyces Pombe Sst2 Catalytic Domain And Ubiquitin" 100.00 81 100.00 100.00 1.15e-45 PDB 4K7S "Crystal Structure Of Zn2-hub (human Ubiquitin) Adduct From A Solution 35 Mm Zinc Acetate/1.3 Mm Hub" 100.00 76 100.00 100.00 6.54e-46 PDB 4K7U "Crystal Structure Of Zn2.3-hub (human Ubiquitin) Adduct From A Solution 70 Mm Zinc Acetate/1.3 Mm Hub" 100.00 76 100.00 100.00 6.54e-46 PDB 4K7W "Crystal Structure Of Zn3-hub(human Ubiquitin) Adduct From A Solution 100 Mm Zinc Acetate/1.3 Mm Hub" 100.00 76 100.00 100.00 6.54e-46 PDB 4KSK "Gumby/fam105b In Complex With Ubiquitin" 100.00 80 100.00 100.00 6.85e-46 PDB 4KSL "Gumby/fam105b In Complex With Linear Di-ubiquitin" 100.00 156 100.00 100.00 6.34e-45 PDB 4KZX "Rabbit 40s Ribosomal Subunit In Complex With Eif1." 100.00 156 100.00 100.00 7.65e-46 PDB 4KZY "Rabbit 40s Ribosomal Subunit In Complex With Eif1 And Eif1a." 100.00 156 100.00 100.00 7.65e-46 PDB 4KZZ "Rabbit 40s Ribosomal Subunit In Complex With Mrna, Initiator Trna And Eif1a" 100.00 156 100.00 100.00 7.65e-46 PDB 4LCD "Structure Of An Rsp5xubxsna3 Complex: Mechanism Of Ubiquitin Ligation And Lysine Prioritization By A Hect E3" 97.37 83 100.00 100.00 1.79e-44 PDB 4LDT "The Structure Of H/ceotub1-ubiquitin Aldehyde-ubch5b~ub" 100.00 76 100.00 100.00 6.54e-46 PDB 4LJO "Structure Of An Active Ligase (hoip)/ubiquitin Transfer Complex" 100.00 76 100.00 100.00 6.54e-46 PDB 4LJP "Structure Of An Active Ligase (hoip-h889a)/ubiquitin Transfer Complex" 100.00 76 100.00 100.00 6.54e-46 PDB 4M0W "Crystal Structure Of Sars-cov Papain-like Protease C112s Mutant In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 4MDK "Cdc34-ubiquitin-cc0651 Complex" 100.00 80 100.00 100.00 6.85e-46 PDB 4MM3 "Crystal Structure Of Sars-cov Papain-like Protease Plpro In Complex With Ubiquitin Aldehyde" 98.68 76 100.00 100.00 4.40e-45 PDB 4MSM "Crystal Structure Of Schizosaccharomyces Pombe Amsh-like Protease Sst2 E286a Mutant Bound To Ubiquitin" 100.00 81 100.00 100.00 1.15e-45 PDB 4MSQ "Crystal Structure Of Schizosaccharomyces Pombe Amsh-like Protease Sst2 Catalytic Domain Bound To Ubiquitin" 100.00 81 100.00 100.00 1.15e-45 PDB 4NQK "Structure Of An Ubiquitin Complex" 100.00 79 100.00 100.00 1.20e-45 PDB 4NQL "The Crystal Structure Of The Dub Domain Of Amsh Orthologue, Sst2 From S. Pombe, In Complex With Lysine 63-linked Diubiquitin" 100.00 77 100.00 100.00 7.42e-46 PDB 4P4H "Caught-in-action Signaling Complex Of Rig-i 2card Domain And Mavs Card Domain" 100.00 79 100.00 100.00 1.20e-45 PDB 4PIG "Crystal Structure Of The Ubiquitin K11s Mutant" 100.00 76 98.68 98.68 2.81e-45 PDB 4PIH "X-ray Crystal Structure Of The K33s Mutant Of Ubiquitin" 100.00 76 98.68 98.68 2.81e-45 PDB 4PIJ "X-ray Crystal Structure Of The K11s/k63s Double Mutant Of Ubiquitin" 98.68 75 97.33 97.33 7.90e-44 PDB 4PQT "Insights Into The Mechanism Of Deubiquitination By Jamm Deubiquitinases From Co-crystal Structures Of Enzyme With Substrate And" 100.00 81 100.00 100.00 1.15e-45 PDB 4R62 "Structure Of Rad6~ub" 100.00 78 100.00 100.00 6.20e-46 PDB 4RF0 "Crystal Structure Of The Middle-east Respiratory Syndrome Coronavirus Papain-like Protease In Complex With Ubiquitin (space Gro" 98.68 75 100.00 100.00 4.28e-45 PDB 4RF1 "Crystal Structure Of The Middle-east Respiratory Syndrome Coronavirus Papain-like Protease In Complex With Ubiquitin (space Gro" 98.68 75 100.00 100.00 4.28e-45 PDB 4S1Z "Crystal Structure Of Trabid Nzf1 In Complex With K29 Linked Di- Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 4S22 "Crystal Structure Of K29 Linked Di-ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 4UEL "Uch-l5 In Complex With Ubiquitin-propargyl Bound To The Rpn13 Deubad Domain" 98.68 76 100.00 100.00 4.40e-45 PDB 4UF6 "Uch-l5 In Complex With Ubiquitin-propargyl Bound To An Activating Fragment Of Ino80g" 98.68 76 100.00 100.00 4.40e-45 PDB 4UN2 "Crystal Structure Of The Uba Domain Of Dsk2 In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 4UPX "Mammalian 80s Hcv-ires Initiation Complex With Eif5b Pre-like State" 100.00 128 100.00 100.00 4.55e-46 PDB 4UQ1 "Mammalian 80s Hcv-ires Initiation Complex With Eif5b Post-like State" 100.00 128 100.00 100.00 4.55e-46 PDB 4UQ4 "Mammalian 80s Hcv-ires Initiation Complex With Eif5b Post-like State" 100.00 156 100.00 100.00 7.65e-46 PDB 4UQ5 "Mammalian 80s Hcv-ires Initiation Complex With Eif5b Pre-like State" 100.00 156 100.00 100.00 7.65e-46 PDB 4V3K "Rnf38-ubch5b-ub Complex" 100.00 81 100.00 100.00 7.68e-46 PDB 4V3L "Rnf38-ub-ubch5b-ub Complex" 100.00 81 100.00 100.00 7.68e-46 PDB 4W20 "Structure Of The Mammalian 60s Ribosomal Subunit (this Entry Contains The Large Ribosomal Proteins)" 100.00 128 100.00 100.00 4.55e-46 PDB 4W22 "Structure Of The 80s Mammalian Ribosome Bound To Eef2 (this Entry Contains The Large Ribosomal Subunit Proteins)" 100.00 128 100.00 100.00 4.55e-46 PDB 4W23 "Structure Of The 80s Mammalian Ribosome Bound To Eef2 (this Entry Contains The Small Ribosomal Subunit)" 100.00 156 100.00 100.00 7.65e-46 PDB 4W25 "Structure Of The Idle Mammalian Ribosome-sec61 Complex (this Entry Contains The Large Ribosomal Subunit Proteins)" 100.00 128 100.00 100.00 4.55e-46 PDB 4W27 "Structure Of The Translating Mammalian Ribosome-sec61 Complex (this Entry Contains The Large Ribosomal Subunit Proteins)" 100.00 128 100.00 100.00 4.55e-46 PDB 4W28 "Structure Of The Translating Mammalian Ribosome-sec61 Complex (this Entry Contains The Small Ribosomal Subunit)" 100.00 156 100.00 100.00 7.65e-46 PDB 4WHV Rnf8/ubc13c87k~ub 100.00 83 100.00 100.00 1.61e-45 PDB 4WLR "Crystal Structure Of Much37-hrpn13 Ctd-hub Complex" 100.00 76 100.00 100.00 6.54e-46 PDB 4WUR "The Crystal Structure Of The Mers-cov Papain-like Protease (c111s) With Human Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 4WZP "Ser65 Phosphorylated Ubiquitin, Major Conformation" 100.00 76 98.68 98.68 4.64e-45 PDB 4XKL "Crystal Structure Of Ndp52 Zf2 In Complex With Mono-ubiquitin" 100.00 80 100.00 100.00 1.02e-45 PDB 4XOF "Observing The Overall Rocking Motion Of A Protein In A Crystal - Orthorhombic Ubiquitin Crystals Without Zinc." 100.00 76 100.00 100.00 6.54e-46 PDB 4XOK "Observing The Overall Rocking Motion Of A Protein In A Crystal." 100.00 76 100.00 100.00 6.54e-46 PDB 4XOL "Observing The Overall Rocking Motion Of A Protein In A Crystal - Cubic Ubiquitin Crystals." 100.00 76 100.00 100.00 6.54e-46 PDB 4XYZ "Crystal Structure Of K33 Linked Di-ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 4Y1H "Crystal Structure Of K33 Linked Tri-ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 4Z9S "Non-covalent Assembly Of Monoubiquitin That Mimics K11 Poly-ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 4ZFR "Catalytic Domain Of Sst2 F403a Mutant Bound To Ubiquitin" 100.00 81 100.00 100.00 1.15e-45 PDB 4ZFT "Catalytic Domain Of Sst2 F403w Mutant Bound To Ubiquitin" 100.00 81 100.00 100.00 1.15e-45 PDB 4ZPZ "Crystal Structure Of Semi-synthetic Ubiquitin With Phospho-ser65 And Ala46cys" 100.00 76 97.37 97.37 1.81e-44 PDB 5A5B "Structure Of The 26s Proteasome-ubp6 Complex" 98.68 76 100.00 100.00 4.40e-45 PDB 5AF4 "Structure Of Lys33-linked Diub" 100.00 76 98.68 100.00 1.56e-45 PDB 5AF5 "Structure Of Lys33-linked Triub S.g. P 212121" 96.05 73 98.63 100.00 5.42e-43 PDB 5AF6 "Structure Of Lys33-linked Diub Bound To Trabid Nzf1" 100.00 76 98.68 100.00 1.56e-45 PDB 5AIT "A Complex Of Of Rnf4-ring Domain, Ubev2, Ubc13-ub (isopeptide Crosslink)" 100.00 76 100.00 100.00 6.54e-46 PDB 5AIU "A Complex Of Rnf4-ring Domain, Ubc13-ub (isopeptide Crosslink)" 100.00 76 100.00 100.00 6.54e-46 PDB 5CAW "Structure Of Pediculus Humanus Parkin Bound To Phospho-ubiquitin" 98.68 76 98.67 98.67 2.77e-44 DBJ BAA03983 "polyubiquitin [Rattus norvegicus]" 100.00 305 100.00 100.00 3.25e-43 DBJ BAA09860 "polyubiquitin [Homo sapiens]" 100.00 611 98.68 98.68 1.53e-40 DBJ BAA11842 "ubiquitin [Cavia porcellus]" 100.00 311 100.00 100.00 3.52e-43 DBJ BAA11843 "ubiquitin extention protein [Cavia porcellus]" 100.00 156 100.00 100.00 7.65e-46 DBJ BAA23486 "polyubiquitin [Homo sapiens]" 100.00 609 98.68 98.68 8.23e-41 EMBL CAA25706 "unnamed protein product [Saccharomyces cerevisiae]" 50.00 191 100.00 100.00 4.38e-16 EMBL CAA26488 "unnamed protein product [Gallus gallus]" 100.00 157 98.68 98.68 3.90e-44 EMBL CAA28495 "ubiquitin [Homo sapiens]" 100.00 229 100.00 100.00 5.18e-44 EMBL CAA30183 "unnamed protein product [Dictyostelium discoideum]" 100.00 128 97.37 97.37 7.90e-44 EMBL CAA30815 "unnamed protein product [Cricetulus sp.]" 93.42 223 100.00 100.00 5.43e-40 GB AAA02769 "polyprotein [Bovine viral diarrhea virus 1-Osloss]" 98.68 3975 97.33 100.00 2.69e-39 GB AAA28154 "polyubiquitin [Caenorhabditis elegans]" 100.00 838 97.37 98.68 1.06e-39 GB AAA28997 "ubiquitin [Drosophila melanogaster]" 100.00 231 100.00 100.00 4.85e-44 GB AAA28998 "ubiquitin-hybrid protein precursor [Drosophila melanogaster]" 100.00 156 100.00 100.00 1.42e-45 GB AAA28999 "ubiquitin, partial [Drosophila melanogaster]" 100.00 76 100.00 100.00 6.54e-46 PIR I50437 "polyubiquitin 4 - chicken [Gallus gallus]" 100.00 305 100.00 100.00 3.25e-43 PIR I51568 "polyubiquitin - African clawed frog (fragment)" 100.00 167 100.00 100.00 1.00e-44 PIR I65237 "ubiquitin / ribosomal protein L40, cytosolic [validated] - rat" 100.00 128 100.00 100.00 4.55e-46 PIR JN0790 "ubiquitin/ribosomal protein CEP52 fusion protein - Leishmania major" 100.00 128 97.37 98.68 3.43e-45 PIR S13928 "ubiquitin precursor - chicken [Gallus gallus]" 100.00 229 100.00 100.00 5.35e-44 PRF 0412265A ubiquitin 98.68 75 98.67 98.67 1.80e-44 PRF 1101405A "ubiquitin precursor" 50.00 191 100.00 100.00 4.33e-16 PRF 1212243A "ubiquitin S1" 100.00 76 100.00 100.00 6.54e-46 PRF 1212243B "ubiquitin S5" 92.11 77 98.57 98.57 1.12e-40 PRF 1212243C "ubiquitin S3" 100.00 76 100.00 100.00 6.54e-46 REF NP_001005123 "ubiquitin-60S ribosomal protein L40 [Xenopus (Silurana) tropicalis]" 100.00 128 100.00 100.00 4.55e-46 REF NP_001006688 "ubiquitin C [Xenopus (Silurana) tropicalis]" 100.00 609 100.00 100.00 1.45e-41 REF NP_001009117 "polyubiquitin-B [Pan troglodytes]" 100.00 229 100.00 100.00 5.18e-44 REF NP_001009202 "polyubiquitin-B [Ovis aries]" 100.00 305 98.68 100.00 5.72e-43 REF NP_001009286 "ubiquitin-60S ribosomal protein L40 [Ovis aries]" 100.00 128 100.00 100.00 4.55e-46 SP P0C273 "RecName: Full=Ubiquitin-60S ribosomal protein L40; AltName: Full=Ubiquitin A-52 residue ribosomal protein fusion product 1; Con" 100.00 128 100.00 100.00 4.55e-46 SP P0C275 "RecName: Full=Ubiquitin-60S ribosomal protein L40; AltName: Full=Ubiquitin A-52 residue ribosomal protein fusion product 1; Con" 100.00 128 100.00 100.00 4.55e-46 SP P0C276 "RecName: Full=Ubiquitin-60S ribosomal protein L40; AltName: Full=Ubiquitin A-52 residue ribosomal protein fusion product 1; Con" 100.00 128 100.00 100.00 4.55e-46 SP P0CG47 "RecName: Full=Polyubiquitin-B; Contains: RecName: Full=Ubiquitin; Flags: Precursor" 100.00 229 100.00 100.00 5.18e-44 SP P0CG48 "RecName: Full=Polyubiquitin-C; Contains: RecName: Full=Ubiquitin; Flags: Precursor" 100.00 685 100.00 100.00 2.13e-41 TPD FAA00319 "TPA: polyubiquitin [Cryptococcus neoformans var. neoformans B-3501A]" 100.00 456 97.37 98.68 1.11e-40 TPE CEL68433 "TPA: ubiquitin / ribosomal protein CEP52 fusion protein, putative [Neospora caninum Liverpool]" 100.00 129 98.68 100.00 1.60e-45 TPE CEL70397 "TPA: Ubiquitin, related [Neospora caninum Liverpool]" 100.00 535 98.68 100.00 3.17e-41 TPE CEL75964 "TPA: ubiquitin / ribosomal protein CEP52 fusion protein, putative [Toxoplasma gondii VEG]" 100.00 129 98.68 100.00 1.60e-45 TPE CEL78064 "TPA: polyubiquitin, putative [Toxoplasma gondii VEG]" 100.00 307 98.68 100.00 1.16e-42 TPG DAA18802 "TPA: polyubiquitin [Bos taurus]" 100.00 305 100.00 100.00 3.43e-43 TPG DAA20663 "TPA: ubiquitin C [Bos taurus]" 98.68 314 98.67 100.00 1.30e-41 TPG DAA20672 "TPA: ubiquitin B-like [Bos taurus]" 100.00 77 98.68 98.68 4.88e-45 TPG DAA24675 "TPA: 40S ribosomal protein S27a [Bos taurus]" 100.00 156 100.00 100.00 7.65e-46 TPG DAA28295 "TPA: ubiquitin and ribosomal protein L40 [Bos taurus]" 100.00 128 100.00 100.00 4.55e-46 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $Ubiquitin Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name _Details $Ubiquitin 'recombinant technology' . Escherichia coli 'BL21 (DE3)' pET His-tagged stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_doubly_labeled_His _Saveframe_category sample _Sample_type solution _Details His-tagged loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $Ubiquitin 1 mM '[U-95% 13C; U-95% 15N]' TRIS 30 mM 'natural abundance' 'sodium chloride' 150 mM 'natural abundance' D2O 5 % [U-2H] H2O 95 % 'natural abundance' stop_ save_ save_doubly_labeled _Saveframe_category sample _Sample_type solution _Details 'purchased from Cambridge Isotope Laboratories, Andover, MA' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $Ubiquitin 0.85 mM 'natural abundance' 'potassium phosphate' 25 mM 'natural abundance' 'sodium chloride' 100 mM 'natural abundance' D2O 5 % [U-2H] H2O 95 % 'natural abundance' stop_ save_ save_triply_labeled _Saveframe_category sample _Sample_type solution _Details 'purchased from VLI Research Inc., Malvern, PA' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $Ubiquitin 1.2 mM '[U-100% 13C; U-100% 15N; 70% 2H]' 'potassium phosphate' 50 mM 'natural abundance' D2O 5 % [U-2H] H2O 95 % 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_xwinnmr _Saveframe_category software _Name xwinnmr _Version . loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . stop_ loop_ _Task collection processing stop_ _Details . save_ save_jeval _Saveframe_category software _Name jeval _Version . loop_ _Vendor _Address _Electronic_address 'JM Schmidt' 'Dept. of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, United Kingdom' j.m.schmidt@kent.ac.uk stop_ loop_ _Task 'coupling constant extraction' 'data analysis' 'multiplet simulation' stop_ _Details 'Matlab-based suite of utilities for the display, selection, and simulation of 2D multiplet projections from multi-dimensional NMR spectra' save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DRX _Field_strength 500 _Details '1H{13C,15N}-triple-resonance three-axis PFG probe' save_ save_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DRX _Field_strength 600 _Details 'cryogenically cooled triple-resonance z-gradient probe' save_ save_spectrometer_3 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 600 _Details 'room-temperature and cryogenic triple-resonance z-gradient probes' save_ save_spectrometer_4 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 800 _Details 'room-temperature and cryogenic triple-resonance z-gradient probes' save_ save_spectrometer_5 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model INOVA _Field_strength 600 _Details '1H{13C,15N}-triple-resonance z-axis PFG probe' save_ ############################# # NMR applied experiments # ############################# save_3D_Ca-coupled_[15N,1H]-TROSY-HNCO_1 _Saveframe_category NMR_applied_experiment _Experiment_name '3D Ca-coupled [15N,1H]-TROSY-HNCO' _Sample_label $doubly_labeled_His save_ save_2D_HN(COCA-J)_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D HN(COCA-J)' _Sample_label $doubly_labeled save_ save_3D_Cb-coupled_[15N,1H]-TROSY-HN(CO)CA_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D Cb-coupled [15N,1H]-TROSY-HN(CO)CA' _Sample_label $triply_labeled save_ save_2D_IPAP-type_HN(CO-a/b-NCa-J)-TROSY_4 _Saveframe_category NMR_applied_experiment _Experiment_name '2D IPAP-type HN(CO-a/b-NCa-J)-TROSY' _Sample_label $doubly_labeled_His save_ save_3D_Ha-coupled_ct-[15N,1H]-TROSY-HN(CO)CA_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D Ha-coupled ct-[15N,1H]-TROSY-HN(CO)CA' _Sample_label $doubly_labeled_His save_ save_3D_1Ha-13Ca_multiple-quantum_HCAN_quantitative_J_correlation_6 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1Ha-13Ca multiple-quantum HCAN quantitative J correlation' _Sample_label $doubly_labeled_His save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 7.6 . pH pressure 1 . atm temperature 300 . K stop_ save_ save_sample_conditions_2 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 6.4 . pH pressure 1 . atm temperature 300 . K stop_ save_ save_sample_conditions_3 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 6.0 . pH pressure 1 . atm temperature 300 . K stop_ save_ ######################## # Coupling constants # ######################## save_1JCaCO _Saveframe_category coupling_constants _Details . loop_ _Experiment_label '3D Ca-coupled [15N,1H]-TROSY-HNCO' '2D HN(COCA-J)' stop_ _Sample_conditions_label $sample_conditions_1 _Spectrometer_frequency_1H 600 _Mol_system_component_name protein _Text_data_format . _Text_data . loop_ _Coupling_constant_ID _Coupling_constant_code _Atom_one_residue_seq_code _Atom_one_residue_label _Atom_one_name _Atom_two_residue_seq_code _Atom_two_residue_label _Atom_two_name _Coupling_constant_value _Coupling_constant_min_value _Coupling_constant_max_value _Coupling_constant_value_error 1 1JCACO 1 MET CA 1 MET C 51.43 . . 0.50 2 1JCACO 2 GLN CA 2 GLN C 53.13 . . 0.50 3 1JCACO 3 ILE CA 3 ILE C 51.24 . . 0.50 4 1JCACO 4 PHE CA 4 PHE C 52.96 . . 0.50 5 1JCACO 5 VAL CA 5 VAL C 52.94 . . 0.50 6 1JCACO 6 LYS CA 6 LYS C 52.73 . . 0.50 7 1JCACO 7 THR CA 7 THR C 51.80 . . 0.50 8 1JCACO 8 LEU CA 8 LEU C 52.89 . . 0.50 9 1JCACO 9 THR CA 9 THR C 52.99 . . 0.50 10 1JCACO 10 GLY CA 10 GLY C 52.64 . . 0.50 11 1JCACO 11 LYS CA 11 LYS C 51.76 . . 0.50 12 1JCACO 12 THR CA 12 THR C 52.26 . . 0.50 13 1JCACO 13 ILE CA 13 ILE C 52.16 . . 0.50 14 1JCACO 14 THR CA 14 THR C 52.11 . . 0.50 15 1JCACO 15 LEU CA 15 LEU C 52.53 . . 0.50 16 1JCACO 16 GLU CA 16 GLU C 52.60 . . 0.50 17 1JCACO 17 VAL CA 17 VAL C 52.17 . . 0.50 18 1JCACO 19 PRO CA 19 PRO C 54.85 . . 0.50 19 1JCACO 20 SER CA 20 SER C 52.36 . . 0.50 20 1JCACO 21 ASP CA 21 ASP C 52.83 . . 0.50 21 1JCACO 22 THR CA 22 THR C 51.54 . . 0.50 22 1JCACO 24 GLU CA 24 GLU C 52.56 . . 0.50 23 1JCACO 25 ASN CA 25 ASN C 52.79 . . 0.50 24 1JCACO 26 VAL CA 26 VAL C 52.91 . . 0.50 25 1JCACO 27 LYS CA 27 LYS C 52.47 . . 0.50 26 1JCACO 28 ALA CA 28 ALA C 52.63 . . 0.50 27 1JCACO 29 LYS CA 29 LYS C 52.23 . . 0.50 28 1JCACO 30 ILE CA 30 ILE C 52.58 . . 0.50 29 1JCACO 31 GLN CA 31 GLN C 52.38 . . 0.50 30 1JCACO 32 ASP CA 32 ASP C 53.23 . . 0.50 31 1JCACO 33 LYS CA 33 LYS C 53.58 . . 0.50 32 1JCACO 34 GLU CA 34 GLU C 53.69 . . 0.50 33 1JCACO 35 GLY CA 35 GLY C 52.44 . . 0.50 34 1JCACO 38 PRO CA 38 PRO C 55.38 . . 0.50 35 1JCACO 39 ASP CA 39 ASP C 53.12 . . 0.50 36 1JCACO 40 GLN CA 40 GLN C 52.29 . . 0.50 37 1JCACO 41 GLN CA 41 GLN C 53.62 . . 0.50 38 1JCACO 42 ARG CA 42 ARG C 52.67 . . 0.50 39 1JCACO 43 LEU CA 43 LEU C 53.18 . . 0.50 40 1JCACO 44 ILE CA 44 ILE C 53.47 . . 0.50 41 1JCACO 45 PHE CA 45 PHE C 53.20 . . 0.50 42 1JCACO 46 ALA CA 46 ALA C 52.58 . . 0.50 43 1JCACO 47 GLY CA 47 GLY C 52.87 . . 0.50 44 1JCACO 48 LYS CA 48 LYS C 52.90 . . 0.50 45 1JCACO 49 GLN CA 49 GLN C 51.98 . . 0.50 46 1JCACO 50 LEU CA 50 LEU C 53.23 . . 0.50 47 1JCACO 51 GLU CA 51 GLU C 53.41 . . 0.50 48 1JCACO 53 GLY CA 53 GLY C 52.38 . . 0.50 49 1JCACO 54 ARG CA 54 ARG C 51.84 . . 0.50 50 1JCACO 55 THR CA 55 THR C 52.25 . . 0.50 51 1JCACO 56 LEU CA 56 LEU C 53.39 . . 0.50 52 1JCACO 57 SER CA 57 SER C 51.58 . . 0.50 53 1JCACO 58 ASP CA 58 ASP C 52.65 . . 0.50 54 1JCACO 59 TYR CA 59 TYR C 51.45 . . 0.50 55 1JCACO 60 ASN CA 60 ASN C 52.81 . . 0.50 56 1JCACO 61 ILE CA 61 ILE C 53.86 . . 0.50 57 1JCACO 62 GLN CA 62 GLN C 51.72 . . 0.50 58 1JCACO 63 LYS CA 63 LYS C 52.30 . . 0.50 59 1JCACO 64 GLU CA 64 GLU C 52.65 . . 0.50 60 1JCACO 65 SER CA 65 SER C 51.01 . . 0.50 61 1JCACO 66 THR CA 66 THR C 52.21 . . 0.50 62 1JCACO 67 LEU CA 67 LEU C 51.71 . . 0.50 63 1JCACO 68 HIS CA 68 HIS C 53.04 . . 0.50 64 1JCACO 69 LEU CA 69 LEU C 52.66 . . 0.50 65 1JCACO 70 VAL CA 70 VAL C 52.26 . . 0.50 66 1JCACO 71 LEU CA 71 LEU C 52.31 . . 0.50 67 1JCACO 72 ARG CA 72 ARG C 52.44 . . 0.50 68 1JCACO 73 LEU CA 73 LEU C 52.34 . . 0.50 69 1JCACO 74 ARG CA 74 ARG C 52.74 . . 0.50 70 1JCACO 75 GLY CA 75 GLY C 52.26 . . 0.50 stop_ save_ save_1JCaN_ _Saveframe_category coupling_constants _Details . loop_ _Experiment_label '2D IPAP-type HN(CO-a/b-NCa-J)-TROSY' '3D 1Ha-13Ca multiple-quantum HCAN quantitative J correlation' stop_ _Sample_conditions_label $sample_conditions_1 _Spectrometer_frequency_1H 600 _Mol_system_component_name protein _Text_data_format . _Text_data . loop_ _Coupling_constant_ID _Coupling_constant_code _Atom_one_residue_seq_code _Atom_one_residue_label _Atom_one_name _Atom_two_residue_seq_code _Atom_two_residue_label _Atom_two_name _Coupling_constant_value _Coupling_constant_min_value _Coupling_constant_max_value _Coupling_constant_value_error 1 1JCAN 2 GLN CA 2 GLN N 11.21 . . 0.50 2 1JCAN 3 ILE CA 3 ILE N 11.57 . . 0.50 3 1JCAN 4 PHE CA 4 PHE N 12.06 . . 0.50 4 1JCAN 5 VAL CA 5 VAL N 10.41 . . 0.50 5 1JCAN 6 LYS CA 6 LYS N 10.43 . . 0.50 6 1JCAN 7 THR CA 7 THR N 12.36 . . 0.50 7 1JCAN 8 LEU CA 8 LEU N 9.59 . . 0.50 8 1JCAN 9 THR CA 9 THR N 10.47 . . 0.50 9 1JCAN 10 GLY CA 10 GLY N 10.65 . . 0.50 10 1JCAN 11 LYS CA 11 LYS N 11.45 . . 0.50 11 1JCAN 12 THR CA 12 THR N 11.03 . . 0.50 12 1JCAN 13 ILE CA 13 ILE N 10.62 . . 0.50 13 1JCAN 14 THR CA 14 THR N 11.09 . . 0.50 14 1JCAN 15 LEU CA 15 LEU N 11.35 . . 0.50 15 1JCAN 16 GLU CA 16 GLU N 10.04 . . 0.50 16 1JCAN 17 VAL CA 17 VAL N 11.95 . . 0.50 17 1JCAN 18 GLU CA 18 GLU N 11.94 . . 0.50 18 1JCAN 19 PRO CA 19 PRO N 9.44 . . 0.50 19 1JCAN 20 SER CA 20 SER N 10.98 . . 0.50 20 1JCAN 21 ASP CA 21 ASP N 11.35 . . 0.50 21 1JCAN 22 THR CA 22 THR N 12.74 . . 0.50 22 1JCAN 23 ILE CA 23 ILE N 9.79 . . 0.50 23 1JCAN 25 ASN CA 25 ASN N 10.05 . . 0.50 24 1JCAN 26 VAL CA 26 VAL N 9.58 . . 0.50 25 1JCAN 27 LYS CA 27 LYS N 9.71 . . 0.50 26 1JCAN 28 ALA CA 28 ALA N 9.63 . . 0.50 27 1JCAN 29 LYS CA 29 LYS N 9.74 . . 0.50 28 1JCAN 30 ILE CA 30 ILE N 9.83 . . 0.50 29 1JCAN 31 GLN CA 31 GLN N 9.79 . . 0.50 30 1JCAN 32 ASP CA 32 ASP N 9.90 . . 0.50 31 1JCAN 33 LYS CA 33 LYS N 9.85 . . 0.50 32 1JCAN 34 GLU CA 34 GLU N 9.72 . . 0.50 33 1JCAN 35 GLY CA 35 GLY N 10.33 . . 0.50 34 1JCAN 36 ILE CA 36 ILE N 10.78 . . 0.50 35 1JCAN 37 PRO CA 37 PRO N 11.66 . . 0.50 36 1JCAN 38 PRO CA 38 PRO N 9.49 . . 0.50 37 1JCAN 39 ASP CA 39 ASP N 10.07 . . 0.50 38 1JCAN 40 GLN CA 40 GLN N 9.91 . . 0.50 39 1JCAN 41 GLN CA 41 GLN N 11.30 . . 0.50 40 1JCAN 42 ARG CA 42 ARG N 10.39 . . 0.50 41 1JCAN 43 LEU CA 43 LEU N 10.59 . . 0.50 42 1JCAN 44 ILE CA 44 ILE N 10.69 . . 0.50 43 1JCAN 45 PHE CA 45 PHE N 10.59 . . 0.50 44 1JCAN 46 ALA CA 46 ALA N 8.87 . . 0.50 45 1JCAN 47 GLY CA 47 GLY N 10.80 . . 0.50 46 1JCAN 48 LYS CA 48 LYS N 11.26 . . 0.50 47 1JCAN 49 GLN CA 49 GLN N 10.78 . . 0.50 48 1JCAN 50 LEU CA 50 LEU N 10.86 . . 0.50 49 1JCAN 51 GLU CA 51 GLU N 11.16 . . 0.50 50 1JCAN 52 ASP CA 52 ASP N 10.54 . . 0.50 51 1JCAN 54 ARG CA 54 ARG N 12.02 . . 0.50 52 1JCAN 55 THR CA 55 THR N 12.72 . . 0.50 53 1JCAN 56 LEU CA 56 LEU N 10.00 . . 0.50 54 1JCAN 57 SER CA 57 SER N 10.21 . . 0.50 55 1JCAN 58 ASP CA 58 ASP N 9.77 . . 0.50 56 1JCAN 59 TYR CA 59 TYR N 10.36 . . 0.50 57 1JCAN 60 ASN CA 60 ASN N 9.86 . . 0.50 58 1JCAN 61 ILE CA 61 ILE N 10.80 . . 0.50 59 1JCAN 62 GLN CA 62 GLN N 12.03 . . 0.50 60 1JCAN 63 LYS CA 63 LYS N 11.49 . . 0.50 61 1JCAN 64 GLU CA 64 GLU N 9.49 . . 0.50 62 1JCAN 65 SER CA 65 SER N 12.76 . . 0.50 63 1JCAN 66 THR CA 66 THR N 11.02 . . 0.50 64 1JCAN 67 LEU CA 67 LEU N 11.14 . . 0.50 65 1JCAN 68 HIS CA 68 HIS N 11.51 . . 0.50 66 1JCAN 69 LEU CA 69 LEU N 10.42 . . 0.50 67 1JCAN 70 VAL CA 70 VAL N 10.90 . . 0.50 68 1JCAN 71 LEU CA 71 LEU N 10.96 . . 0.50 69 1JCAN 72 ARG CA 72 ARG N 10.57 . . 0.50 70 1JCAN 73 LEU CA 73 LEU N 10.61 . . 0.50 71 1JCAN 74 ARG CA 74 ARG N 10.73 . . 0.50 72 1JCAN 75 GLY CA 75 GLY N 11.37 . . 0.50 73 1JCAN 76 GLY CA 76 GLY N 11.09 . . 0.50 stop_ save_ save_1JCaHa _Saveframe_category coupling_constants _Details ; 28-ms constant-time Ca evolution in 3D TROSY experiments 2D on FRXN only ; loop_ _Experiment_label '3D Ha-coupled ct-[15N,1H]-TROSY-HN(CO)CA' stop_ _Sample_conditions_label $sample_conditions_1 _Spectrometer_frequency_1H 600 _Mol_system_component_name protein _Text_data_format . _Text_data . loop_ _Coupling_constant_ID _Coupling_constant_code _Atom_one_residue_seq_code _Atom_one_residue_label _Atom_one_name _Atom_two_residue_seq_code _Atom_two_residue_label _Atom_two_name _Coupling_constant_value _Coupling_constant_min_value _Coupling_constant_max_value _Coupling_constant_value_error 1 1JCAHA 2 GLN CA 2 GLN HA 144.33 . . 0.50 2 1JCAHA 3 ILE CA 3 ILE HA 137.73 . . 0.50 3 1JCAHA 4 PHE CA 4 PHE HA 144.36 . . 0.50 4 1JCAHA 5 VAL CA 5 VAL HA 142.52 . . 0.50 5 1JCAHA 6 LYS CA 6 LYS HA 142.78 . . 0.50 6 1JCAHA 12 THR CA 12 THR HA 143.50 . . 0.50 7 1JCAHA 13 ILE CA 13 ILE HA 141.18 . . 0.50 8 1JCAHA 14 THR CA 14 THR HA 143.42 . . 0.50 9 1JCAHA 15 LEU CA 15 LEU HA 141.32 . . 0.50 10 1JCAHA 16 GLU CA 16 GLU HA 143.28 . . 0.50 11 1JCAHA 17 VAL CA 17 VAL HA 139.55 . . 0.50 12 1JCAHA 19 PRO CA 19 PRO HA 149.92 . . 0.50 13 1JCAHA 20 SER CA 20 SER HA 141.44 . . 0.50 14 1JCAHA 21 ASP CA 21 ASP HA 144.29 . . 0.50 15 1JCAHA 22 THR CA 22 THR HA 140.10 . . 0.50 16 1JCAHA 24 GLU CA 24 GLU HA 146.71 . . 0.50 17 1JCAHA 25 ASN CA 25 ASN HA 149.69 . . 0.50 18 1JCAHA 26 VAL CA 26 VAL HA 146.09 . . 0.50 19 1JCAHA 27 LYS CA 27 LYS HA 150.85 . . 0.50 20 1JCAHA 28 ALA CA 28 ALA HA 147.98 . . 0.50 21 1JCAHA 29 LYS CA 29 LYS HA 148.33 . . 0.50 22 1JCAHA 30 ILE CA 30 ILE HA 145.26 . . 0.50 23 1JCAHA 31 GLN CA 31 GLN HA 148.85 . . 0.50 24 1JCAHA 32 ASP CA 32 ASP HA 150.10 . . 0.50 25 1JCAHA 33 LYS CA 33 LYS HA 146.66 . . 0.50 26 1JCAHA 34 GLU CA 34 GLU HA 143.46 . . 0.50 27 1JCAHA 38 PRO CA 38 PRO HA 148.16 . . 0.50 28 1JCAHA 39 ASP CA 39 ASP HA 145.01 . . 0.50 29 1JCAHA 40 GLN CA 40 GLN HA 141.58 . . 0.50 30 1JCAHA 41 GLN CA 41 GLN HA 142.18 . . 0.50 31 1JCAHA 42 ARG CA 42 ARG HA 141.02 . . 0.50 32 1JCAHA 43 LEU CA 43 LEU HA 142.42 . . 0.50 33 1JCAHA 44 ILE CA 44 ILE HA 142.83 . . 0.50 34 1JCAHA 46 ALA CA 46 ALA HA 135.58 . . 0.50 35 1JCAHA 48 LYS CA 48 LYS HA 141.79 . . 0.50 36 1JCAHA 49 GLN CA 49 GLN HA 142.37 . . 0.50 37 1JCAHA 50 LEU CA 50 LEU HA 141.40 . . 0.50 38 1JCAHA 51 GLU CA 51 GLU HA 141.52 . . 0.50 39 1JCAHA 54 ARG CA 54 ARG HA 140.59 . . 0.50 40 1JCAHA 55 THR CA 55 THR HA 138.46 . . 0.50 41 1JCAHA 56 LEU CA 56 LEU HA 146.75 . . 0.50 42 1JCAHA 57 SER CA 57 SER HA 146.71 . . 0.50 43 1JCAHA 58 ASP CA 58 ASP HA 150.11 . . 0.50 44 1JCAHA 59 TYR CA 59 TYR HA 141.68 . . 0.50 45 1JCAHA 60 ASN CA 60 ASN HA 136.37 . . 0.50 46 1JCAHA 61 ILE CA 61 ILE HA 141.55 . . 0.50 47 1JCAHA 62 GLN CA 62 GLN HA 140.23 . . 0.50 48 1JCAHA 63 LYS CA 63 LYS HA 142.74 . . 0.50 49 1JCAHA 64 GLU CA 64 GLU HA 133.16 . . 0.50 50 1JCAHA 65 SER CA 65 SER HA 144.74 . . 0.50 51 1JCAHA 66 THR CA 66 THR HA 143.24 . . 0.50 52 1JCAHA 67 LEU CA 67 LEU HA 141.68 . . 0.50 53 1JCAHA 68 HIS CA 68 HIS HA 142.86 . . 0.50 54 1JCAHA 69 LEU CA 69 LEU HA 142.50 . . 0.50 55 1JCAHA 70 VAL CA 70 VAL HA 138.81 . . 0.50 56 1JCAHA 71 LEU CA 71 LEU HA 143.36 . . 0.50 57 1JCAHA 72 ARG CA 72 ARG HA 141.70 . . 0.50 stop_ save_ save_1JCaCb _Saveframe_category coupling_constants _Details 'Ca evolution time typically 70-90ms' loop_ _Experiment_label '3D Cb-coupled [15N,1H]-TROSY-HN(CO)CA' stop_ _Sample_conditions_label $sample_conditions_1 _Spectrometer_frequency_1H 800 _Mol_system_component_name protein _Text_data_format . _Text_data . loop_ _Coupling_constant_ID _Coupling_constant_code _Atom_one_residue_seq_code _Atom_one_residue_label _Atom_one_name _Atom_two_residue_seq_code _Atom_two_residue_label _Atom_two_name _Coupling_constant_value _Coupling_constant_min_value _Coupling_constant_max_value _Coupling_constant_value_error 1 1JCACB 1 MET CA 1 MET CB 32.39 . . 0.50 2 1JCACB 2 GLN CA 2 GLN CB 34.70 . . 0.50 3 1JCACB 3 ILE CA 3 ILE CB 34.29 . . 0.50 4 1JCACB 4 PHE CA 4 PHE CB 33.07 . . 0.50 5 1JCACB 5 VAL CA 5 VAL CB 35.24 . . 0.50 6 1JCACB 6 LYS CA 6 LYS CB 34.88 . . 0.50 7 1JCACB 7 THR CA 7 THR CB 37.77 . . 0.50 8 1JCACB 8 LEU CA 8 LEU CB 33.49 . . 0.50 9 1JCACB 9 THR CA 9 THR CB 39.89 . . 0.50 10 1JCACB 11 LYS CA 11 LYS CB 33.72 . . 0.50 11 1JCACB 12 THR CA 12 THR CB 38.70 . . 0.50 12 1JCACB 13 ILE CA 13 ILE CB 34.49 . . 0.50 13 1JCACB 14 THR CA 14 THR CB 38.44 . . 0.50 14 1JCACB 15 LEU CA 15 LEU CB 32.67 . . 0.50 15 1JCACB 16 GLU CA 16 GLU CB 36.12 . . 0.50 16 1JCACB 17 VAL CA 17 VAL CB 32.91 . . 0.50 17 1JCACB 19 PRO CA 19 PRO CB 31.85 . . 0.50 18 1JCACB 20 SER CA 20 SER CB 37.65 . . 0.50 19 1JCACB 21 ASP CA 21 ASP CB 36.10 . . 0.50 20 1JCACB 22 THR CA 22 THR CB 37.17 . . 0.50 21 1JCACB 24 GLU CA 24 GLU CB 33.26 . . 0.50 22 1JCACB 25 ASN CA 25 ASN CB 35.46 . . 0.50 23 1JCACB 26 VAL CA 26 VAL CB 33.23 . . 0.50 24 1JCACB 27 LYS CA 27 LYS CB 33.07 . . 0.50 25 1JCACB 28 ALA CA 28 ALA CB 33.08 . . 0.50 26 1JCACB 29 LYS CA 29 LYS CB 33.28 . . 0.50 27 1JCACB 30 ILE CA 30 ILE CB 33.41 . . 0.50 28 1JCACB 31 GLN CA 31 GLN CB 33.34 . . 0.50 29 1JCACB 32 ASP CA 32 ASP CB 34.47 . . 0.50 30 1JCACB 33 LYS CA 33 LYS CB 32.85 . . 0.50 31 1JCACB 34 GLU CA 34 GLU CB 34.46 . . 0.50 32 1JCACB 38 PRO CA 38 PRO CB 31.49 . . 0.50 33 1JCACB 39 ASP CA 39 ASP CB 35.11 . . 0.50 34 1JCACB 40 GLN CA 40 GLN CB 35.95 . . 0.50 35 1JCACB 41 GLN CA 41 GLN CB 34.60 . . 0.50 36 1JCACB 42 ARG CA 42 ARG CB 35.48 . . 0.50 37 1JCACB 43 LEU CA 43 LEU CB 34.92 . . 0.50 38 1JCACB 44 ILE CA 44 ILE CB 33.65 . . 0.50 39 1JCACB 45 PHE CA 45 PHE CB 34.18 . . 0.50 40 1JCACB 46 ALA CA 46 ALA CB 38.53 . . 0.50 41 1JCACB 48 LYS CA 48 LYS CB 33.89 . . 0.50 42 1JCACB 49 GLN CA 49 GLN CB 35.31 . . 0.50 43 1JCACB 50 LEU CA 50 LEU CB 34.47 . . 0.50 44 1JCACB 51 GLU CA 51 GLU CB 33.80 . . 0.50 45 1JCACB 54 ARG CA 54 ARG CB 34.09 . . 0.50 46 1JCACB 55 THR CA 55 THR CB 37.49 . . 0.50 47 1JCACB 56 LEU CA 56 LEU CB 33.27 . . 0.50 48 1JCACB 57 SER CA 57 SER CB 37.76 . . 0.50 49 1JCACB 58 ASP CA 58 ASP CB 35.03 . . 0.50 50 1JCACB 59 TYR CA 59 TYR CB 35.29 . . 0.50 51 1JCACB 60 ASN CA 60 ASN CB 41.31 . . 0.50 52 1JCACB 61 ILE CA 61 ILE CB 35.41 . . 0.50 53 1JCACB 62 GLN CA 62 GLN CB 34.02 . . 0.50 54 1JCACB 63 LYS CA 63 LYS CB 33.18 . . 0.50 55 1JCACB 64 GLU CA 64 GLU CB 37.64 . . 0.50 56 1JCACB 65 SER CA 65 SER CB 35.77 . . 0.50 57 1JCACB 66 THR CA 66 THR CB 38.26 . . 0.50 58 1JCACB 67 LEU CA 67 LEU CB 34.45 . . 0.50 59 1JCACB 68 HIS CA 68 HIS CB 34.53 . . 0.50 60 1JCACB 69 LEU CA 69 LEU CB 34.84 . . 0.50 61 1JCACB 70 VAL CA 70 VAL CB 34.11 . . 0.50 62 1JCACB 71 LEU CA 71 LEU CB 34.04 . . 0.50 63 1JCACB 72 ARG CA 72 ARG CB 35.20 . . 0.50 64 1JCACB 73 LEU CA 73 LEU CB 34.03 . . 0.50 65 1JCACB 74 ARG CA 74 ARG CB 34.56 . . 0.50 stop_ save_