data_15968 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Backbone chemical shift assignments for the UBA domain of Swa2p ; _BMRB_accession_number 15968 _BMRB_flat_file_name bmr15968.str _Entry_type original _Submission_date 2008-09-29 _Accession_date 2008-09-29 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Matta-Camacho Edna . . 2 Kozlov Guennadi . . 3 Trempe Jean-Francois . . 4 Gehring Kalle . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 46 "13C chemical shifts" 93 "15N chemical shifts" 46 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2009-03-04 update BMRB 'complete entry citation' 2008-11-05 original author 'original release' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Atypical binding of the Swa2p UBA domain to ubiquitin' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 18948116 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Matta-Camacho Edna . . 2 Kozlov Guennadi . . 3 Trempe Jean-Francois . . 4 Gehring Kalle . . stop_ _Journal_abbreviation 'J. Mol. Biol.' _Journal_volume 386 _Journal_issue 2 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 569 _Page_last 577 _Year 2009 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name Swa2p _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'polypeptide chain' $Swa2p stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_Swa2p _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common Swa2p _Molecular_mass . _Mol_thiol_state 'not present' loop_ _Biological_function 'Endocytosis, no clathrin disassembly role in budding yeast.' stop_ _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 52 _Mol_residue_sequence ; GPLGSALVDEVKDMEIARLM SLGLSIEEATEFYENDVTYE RYLEILKSKQKE ; loop_ _Residue_seq_code _Residue_label 1 GLY 2 PRO 3 LEU 4 GLY 5 SER 6 ALA 7 LEU 8 VAL 9 ASP 10 GLU 11 VAL 12 LYS 13 ASP 14 MET 15 GLU 16 ILE 17 ALA 18 ARG 19 LEU 20 MET 21 SER 22 LEU 23 GLY 24 LEU 25 SER 26 ILE 27 GLU 28 GLU 29 ALA 30 THR 31 GLU 32 PHE 33 TYR 34 GLU 35 ASN 36 ASP 37 VAL 38 THR 39 TYR 40 GLU 41 ARG 42 TYR 43 LEU 44 GLU 45 ILE 46 LEU 47 LYS 48 SER 49 LYS 50 GLN 51 LYS 52 GLU stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-03-29 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 1PGY "Solution Structure Of The Uba Domain In Saccharomyces Cerevisiae Protein, Swa2p" 90.38 47 100.00 100.00 6.00e-23 DBJ GAA22540 "K7_Swa2p [Saccharomyces cerevisiae Kyokai no. 7]" 90.38 668 100.00 100.00 3.60e-21 EMBL CAY78820 "Swa2p [Saccharomyces cerevisiae EC1118]" 90.38 668 100.00 100.00 4.02e-21 GB AAB64756 "Ydr320cp [Saccharomyces cerevisiae]" 90.38 668 100.00 100.00 3.98e-21 GB AHY75293 "Swa2p [Saccharomyces cerevisiae YJM993]" 90.38 668 100.00 100.00 3.94e-21 GB AJP38020 "Swa2p [Saccharomyces cerevisiae YJM1078]" 90.38 668 100.00 100.00 3.79e-21 GB AJU58143 "Swa2p [Saccharomyces cerevisiae YJM189]" 90.38 668 100.00 100.00 3.94e-21 GB AJU58836 "Swa2p [Saccharomyces cerevisiae YJM193]" 90.38 668 100.00 100.00 4.02e-21 REF NP_010606 "Swa2p [Saccharomyces cerevisiae S288c]" 90.38 668 100.00 100.00 3.98e-21 SP Q06677 "RecName: Full=Auxilin-like clathrin uncoating factor SWA2; AltName: Full=Bud site selection protein 24; AltName: Full=DnaJ-rela" 90.38 668 100.00 100.00 3.98e-21 TPG DAA12161 "TPA: Swa2p [Saccharomyces cerevisiae S288c]" 90.38 668 100.00 100.00 3.98e-21 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $Swa2p 'baker's yeast' 4932 Eukaryota Metazoa Saccharomyces cerevisiae stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $Swa2p 'recombinant technology' . Escherichia coli . pGEX-6P1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $Swa2p 0.5-1.0 mM '[U-13C; U-15N]' HEPES 20 mM 'natural abundance' 'sodium chloride' 50 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_XEASY _Saveframe_category software _Name XEASY _Version . loop_ _Vendor _Address _Electronic_address 'Bartels et al.' . . stop_ loop_ _Task 'data analysis' stop_ _Details . save_ save_xwinnmr _Saveframe_category software _Name xwinnmr _Version . loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . stop_ loop_ _Task collection stop_ _Details . save_ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_3D_CBCA(CO)NH_1 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $sample_1 save_ save_3D_HNCACB_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 50 . mM pH 7 . pH pressure 1 . atm temperature 303 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio water C 13 protons ppm 4.70 na indirect . . . 0.251449530 water H 1 protons ppm 4.70 internal direct . . . 1 water N 15 protons ppm 4.70 na indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '3D CBCA(CO)NH' '3D HNCACB' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'polypeptide chain' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 6 6 ALA CA C 52.4 0.20 1 2 6 6 ALA CB C 19.2 0.20 1 3 7 7 LEU H H 8.28 0.01 1 4 7 7 LEU CA C 55.5 0.20 1 5 7 7 LEU CB C 42.1 0.20 1 6 7 7 LEU N N 121.6 0.25 1 7 8 8 VAL H H 8.00 0.01 1 8 8 8 VAL CA C 62.6 0.20 1 9 8 8 VAL CB C 32.8 0.20 1 10 8 8 VAL N N 120.4 0.25 1 11 9 9 ASP H H 8.42 0.01 1 12 9 9 ASP CA C 54.3 0.20 1 13 9 9 ASP CB C 40.9 0.20 1 14 9 9 ASP N N 124.1 0.25 1 15 10 10 GLU H H 8.47 0.01 1 16 10 10 GLU CA C 58.5 0.20 1 17 10 10 GLU CB C 29.9 0.20 1 18 10 10 GLU N N 121.8 0.25 1 19 11 11 VAL H H 7.96 0.01 1 20 11 11 VAL CA C 65.4 0.20 1 21 11 11 VAL CB C 31.7 0.20 1 22 11 11 VAL N N 119.9 0.25 1 23 12 12 LYS H H 7.67 0.01 1 24 12 12 LYS CA C 59.4 0.20 1 25 12 12 LYS CB C 32.2 0.20 1 26 12 12 LYS N N 121.8 0.25 1 27 13 13 ASP H H 7.98 0.01 1 28 13 13 ASP CA C 57.4 0.20 1 29 13 13 ASP CB C 40.0 0.20 1 30 13 13 ASP N N 116.5 0.25 1 31 14 14 MET H H 7.87 0.01 1 32 14 14 MET CA C 58.4 0.20 1 33 14 14 MET CB C 32.2 0.20 1 34 14 14 MET N N 119.9 0.25 1 35 15 15 GLU H H 8.35 0.01 1 36 15 15 GLU CA C 58.7 0.20 1 37 15 15 GLU CB C 29.3 0.20 1 38 15 15 GLU N N 121.3 0.25 1 39 16 16 ILE H H 8.52 0.01 1 40 16 16 ILE CA C 65.6 0.20 1 41 16 16 ILE CB C 37.1 0.20 1 42 16 16 ILE N N 121.5 0.25 1 43 17 17 ALA H H 7.82 0.01 1 44 17 17 ALA CA C 55.3 0.20 1 45 17 17 ALA CB C 17.5 0.20 1 46 17 17 ALA N N 122.1 0.25 1 47 18 18 ARG H H 8.06 0.01 1 48 18 18 ARG CA C 60.2 0.20 1 49 18 18 ARG CB C 29.7 0.20 1 50 18 18 ARG N N 119.0 0.25 1 51 19 19 LEU H H 8.23 0.01 1 52 19 19 LEU CA C 59.1 0.20 1 53 19 19 LEU CB C 43.0 0.20 1 54 19 19 LEU N N 122.7 0.25 1 55 20 20 MET H H 8.60 0.01 1 56 20 20 MET CA C 59.3 0.20 1 57 20 20 MET CB C 32.9 0.20 1 58 20 20 MET N N 117.1 0.25 1 59 21 21 SER H H 7.92 0.01 1 60 21 21 SER CA C 61.4 0.20 1 61 21 21 SER CB C 62.7 0.20 1 62 21 21 SER N N 117.3 0.25 1 63 22 22 LEU H H 7.49 0.01 1 64 22 22 LEU CA C 54.5 0.20 1 65 22 22 LEU CB C 41.4 0.20 1 66 22 22 LEU N N 121.3 0.25 1 67 23 23 GLY H H 8.12 0.01 1 68 23 23 GLY CA C 44.9 0.20 1 69 23 23 GLY N N 107.2 0.25 1 70 24 24 LEU H H 6.60 0.01 1 71 24 24 LEU CA C 54.0 0.20 1 72 24 24 LEU CB C 43.8 0.20 1 73 24 24 LEU N N 119.9 0.25 1 74 25 25 SER H H 8.99 0.01 1 75 25 25 SER CA C 57.1 0.20 1 76 25 25 SER CB C 65.5 0.20 1 77 25 25 SER N N 117.6 0.25 1 78 26 26 ILE H H 9.17 0.01 1 79 26 26 ILE CA C 64.7 0.20 1 80 26 26 ILE CB C 37.7 0.20 1 81 26 26 ILE N N 121.3 0.25 1 82 27 27 GLU H H 8.95 0.01 1 83 27 27 GLU CA C 61.1 0.20 1 84 27 27 GLU CB C 28.2 0.20 1 85 27 27 GLU N N 124.9 0.25 1 86 28 28 GLU H H 7.76 0.01 1 87 28 28 GLU CA C 58.6 0.20 1 88 28 28 GLU CB C 31.1 0.20 1 89 28 28 GLU N N 120.7 0.25 1 90 29 29 ALA H H 9.09 0.01 1 91 29 29 ALA CA C 55.1 0.20 1 92 29 29 ALA CB C 20.6 0.20 1 93 29 29 ALA N N 120.2 0.25 1 94 30 30 THR H H 8.25 0.01 1 95 30 30 THR CA C 67.5 0.20 1 96 30 30 THR CB C 68.6 0.20 1 97 30 30 THR N N 114.0 0.25 1 98 31 31 GLU H H 7.53 0.01 1 99 31 31 GLU CA C 59.5 0.20 1 100 31 31 GLU CB C 29.2 0.20 1 101 31 31 GLU N N 121.6 0.25 1 102 32 32 PHE H H 8.26 0.01 1 103 32 32 PHE CA C 61.5 0.20 1 104 32 32 PHE CB C 36.9 0.20 1 105 32 32 PHE N N 118.5 0.25 1 106 33 33 TYR H H 8.64 0.01 1 107 33 33 TYR CA C 62.6 0.20 1 108 33 33 TYR CB C 38.8 0.20 1 109 33 33 TYR N N 121.6 0.25 1 110 34 34 GLU H H 8.60 0.01 1 111 34 34 GLU CA C 58.5 0.20 1 112 34 34 GLU CB C 29.0 0.20 1 113 34 34 GLU N N 118.7 0.25 1 114 35 35 ASN H H 7.62 0.01 1 115 35 35 ASN CA C 53.0 0.20 1 116 35 35 ASN CB C 39.1 0.20 1 117 35 35 ASN N N 117.9 0.25 1 118 36 36 ASP H H 7.83 0.01 1 119 36 36 ASP CA C 55.8 0.20 1 120 36 36 ASP CB C 39.0 0.20 1 121 36 36 ASP N N 113.9 0.25 1 122 37 37 VAL H H 8.37 0.01 1 123 37 37 VAL CA C 63.3 0.20 1 124 37 37 VAL CB C 30.8 0.20 1 125 37 37 VAL N N 122.4 0.25 1 126 38 38 THR H H 6.48 0.01 1 127 38 38 THR CA C 59.7 0.20 1 128 38 38 THR CB C 71.5 0.20 1 129 38 38 THR N N 111.1 0.25 1 130 39 39 TYR H H 9.79 0.01 1 131 39 39 TYR CA C 62.7 0.20 1 132 39 39 TYR CB C 39.4 0.20 1 133 39 39 TYR N N 121.8 0.25 1 134 40 40 GLU H H 8.91 0.01 1 135 40 40 GLU CA C 60.7 0.20 1 136 40 40 GLU CB C 28.8 0.20 1 137 40 40 GLU N N 115.9 0.25 1 138 41 41 ARG H H 7.52 0.01 1 139 41 41 ARG CA C 58.2 0.20 1 140 41 41 ARG CB C 30.2 0.20 1 141 41 41 ARG N N 119.0 0.25 1 142 42 42 TYR H H 8.99 0.01 1 143 42 42 TYR CA C 61.1 0.20 1 144 42 42 TYR CB C 37.7 0.20 1 145 42 42 TYR N N 122.4 0.25 1 146 43 43 LEU H H 8.03 0.01 1 147 43 43 LEU CA C 57.9 0.20 1 148 43 43 LEU CB C 41.5 0.20 1 149 43 43 LEU N N 117.6 0.25 1 150 44 44 GLU H H 7.19 0.01 1 151 44 44 GLU CA C 58.9 0.20 1 152 44 44 GLU CB C 29.3 0.20 1 153 44 44 GLU N N 117.6 0.25 1 154 45 45 ILE H H 7.82 0.01 1 155 45 45 ILE CA C 65.0 0.20 1 156 45 45 ILE CB C 37.7 0.20 1 157 45 45 ILE N N 122.7 0.25 1 158 46 46 LEU H H 8.08 0.01 1 159 46 46 LEU CA C 57.5 0.20 1 160 46 46 LEU CB C 41.6 0.20 1 161 46 46 LEU N N 120.7 0.25 1 162 47 47 LYS H H 7.78 0.01 1 163 47 47 LYS CA C 57.9 0.20 1 164 47 47 LYS CB C 32.2 0.20 1 165 47 47 LYS N N 118.2 0.25 1 166 48 48 SER H H 7.73 0.01 1 167 48 48 SER CA C 60.0 0.20 1 168 48 48 SER CB C 63.5 0.20 1 169 48 48 SER N N 114.0 0.25 1 170 49 49 LYS H H 7.59 0.01 1 171 49 49 LYS CA C 56.7 0.20 1 172 49 49 LYS CB C 32.6 0.20 1 173 49 49 LYS N N 121.3 0.25 1 174 50 50 GLN H H 7.91 0.01 1 175 50 50 GLN CA C 55.9 0.20 1 176 50 50 GLN CB C 29.0 0.20 1 177 50 50 GLN N N 120.1 0.25 1 178 51 51 LYS H H 8.13 0.01 1 179 51 51 LYS CA C 56.1 0.20 1 180 51 51 LYS CB C 33.1 0.20 1 181 51 51 LYS N N 122.4 0.25 1 182 52 52 GLU H H 7.96 0.01 1 183 52 52 GLU CA C 58.0 0.20 1 184 52 52 GLU CB C 31.0 0.20 1 185 52 52 GLU N N 127.2 0.25 1 stop_ save_