data_15993 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; 1H-15N Backbone Chemical shifts Of the C-terminal part of the Phosphoprotein (XD) of Measle Virus ; _BMRB_accession_number 15993 _BMRB_flat_file_name bmr15993.str _Entry_type original _Submission_date 2008-10-20 _Accession_date 2008-10-20 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details '1H-15N Backbone Chemical shifts Of the C-terminal part of the Phosphoprotein (XD) of Measle Virus' loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Bernard Cedric . . 2 Bourhis 'Jean Marie' . . 3 Gely Stephane . . 4 Morelli Xavier . . 5 Longhi Sonia . . 6 Darbon Herv . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 43 "15N chemical shifts" 43 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2009-04-03 original author . stop_ loop_ _Related_BMRB_accession_number _Relationship 15994 'XD Complex with C-Terminal Part of the Nucloeprotein.' stop_ _Original_release_date 2009-04-03 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Interaction between the C-terminal Domains Of N and P Proteins of measles virus investigated by NMR' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 19275899 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Bernard Cedric . . 2 Gely Stephane . . 3 Bourhis Jean-Marie . . 4 Morelli Xavier . . 5 Longhi Sonia . . 6 Darbon Herv . . stop_ _Journal_abbreviation 'FEBS Lett.' _Journal_name_full 'FEBS Lettets' _Journal_volume 583 _Journal_issue 7 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 1084 _Page_last 1089 _Year 2009 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name XD _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label XD $XD stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_XD _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common XD _Molecular_mass . _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 44 _Mol_residue_sequence ; VIRSIIKSSRLEEDRKRYLM TLLDDIKGANDLAKFHQMLV KIIM ; loop_ _Residue_seq_code _Residue_label 1 VAL 2 ILE 3 ARG 4 SER 5 ILE 6 ILE 7 LYS 8 SER 9 SER 10 ARG 11 LEU 12 GLU 13 GLU 14 ASP 15 ARG 16 LYS 17 ARG 18 TYR 19 LEU 20 MET 21 THR 22 LEU 23 LEU 24 ASP 25 ASP 26 ILE 27 LYS 28 GLY 29 ALA 30 ASN 31 ASP 32 LEU 33 ALA 34 LYS 35 PHE 36 HIS 37 GLN 38 MET 39 LEU 40 VAL 41 LYS 42 ILE 43 ILE 44 MET stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-07-08 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 15994 XD 100.00 44 100.00 100.00 5.62e-21 BMRB 16000 XD_domain 97.73 44 100.00 100.00 2.85e-20 PDB 1T6O "Nucleocapsid-Binding Domain Of The Measles Virus P Protein (Amino Acids 457-507) In Complex With Amino Acids 486-505 Of The Mea" 100.00 51 97.73 100.00 1.70e-20 PDB 2K9D "Solution Structure Of The Domain X Of Measle Phosphoprotein" 97.73 44 100.00 100.00 2.85e-20 DBJ BAA09955 "phosphoprotein [Measles virus]" 100.00 507 97.73 100.00 1.33e-19 DBJ BAA09962 "phosphoprotein [Measles virus]" 100.00 507 97.73 100.00 1.33e-19 DBJ BAA33868 "phosphoprotein [Measles virus]" 100.00 507 97.73 100.00 1.33e-19 DBJ BAA33874 "phosphoprotein [Measles virus]" 100.00 507 97.73 100.00 1.33e-19 DBJ BAA34978 "phosphoprotein [Measles virus]" 100.00 507 97.73 100.00 1.33e-19 EMBL CAA34578 "phosphoprotein [Measles virus]" 100.00 507 97.73 100.00 1.33e-19 EMBL CAA34585 "unnamed protein product [Measles virus]" 100.00 507 97.73 100.00 1.33e-19 EMBL CAA91364 "phosphoprotein [Measles virus]" 100.00 507 97.73 100.00 1.33e-19 EMBL CAQ15998 "phosphoprotein [Measles virus MVi/Ibadan.NIE/8.98/9]" 100.00 507 97.73 100.00 1.33e-19 EMBL CAQ15999 "phosphoprotein [Measles virus MVi/Ibadan.NIE/9.98/5]" 100.00 507 97.73 100.00 1.33e-19 GB AAA46434 "phosphoprotein [Measles virus]" 100.00 507 97.73 100.00 1.33e-19 GB AAA46435 "phosphoprotein [Measles virus]" 100.00 507 97.73 100.00 1.33e-19 GB AAA46437 "phosphoprotein [Measles virus]" 100.00 507 97.73 100.00 1.33e-19 GB AAA63284 "P protein [Measles virus]" 100.00 507 97.73 100.00 1.33e-19 GB AAA63285 "P protein [Measles virus]" 100.00 507 97.73 100.00 1.33e-19 REF NP_056919 "phosphoprotein [Measles virus]" 100.00 507 97.73 100.00 1.33e-19 SP P03422 "RecName: Full=Phosphoprotein; Short=Protein P" 100.00 507 97.73 100.00 1.33e-19 SP P35974 "RecName: Full=Phosphoprotein; Short=Protein P" 100.00 507 97.73 100.00 1.33e-19 SP Q9WMB4 "RecName: Full=Phosphoprotein; Short=Protein P" 100.00 507 97.73 100.00 1.33e-19 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $XD Measles 11234 Viruses . Morbillivirus 'Measles virus' stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $XD 'recombinant technology' . Escherichia coli . pET21a stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $XD 1.2 mM '[U-100% 15N]' H2O 90 % 'natural abundance' D2O 10 % 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_xwinnmr _Saveframe_category software _Name xwinnmr _Version . loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . stop_ loop_ _Task collection stop_ _Details . save_ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ save_NMRView _Saveframe_category software _Name NMRView _Version . loop_ _Vendor _Address _Electronic_address 'Johnson, One Moon Scientific' . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 600 _Details Cryo-probe save_ save_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DRX _Field_strength 500 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_2D_1H-15N_HSQC-NOESY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC-NOESY' _Sample_label $sample_1 save_ save_2D_1H-1H_TOCSY_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H TOCSY' _Sample_label $sample_1 save_ save_2D_1H-1H_NOESY_4 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_1 save_ save_3D_1H-15N_NOESY_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-15N NOESY' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 8 . pH pressure 1 . atm temperature 300 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details 'only backbone atoms are deposited' loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio water H 1 protons ppm 4.689 na direct . . . 1 water N 15 protons ppm 4.698 na indirect . . . . stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' '2D 1H-15N HSQC-NOESY' '2D 1H-1H TOCSY' '2D 1H-1H NOESY' '3D 1H-15N NOESY' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name XD _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 463 1 VAL H H 7.568 . 1 2 463 1 VAL N N 124.990 . 1 3 464 2 ILE H H 8.230 . 1 4 464 2 ILE N N 120.553 . 1 5 465 3 ARG H H 8.608 . 1 6 465 3 ARG N N 118.677 . 1 7 466 4 SER H H 7.857 . 1 8 466 4 SER N N 114.311 . 1 9 467 5 ILE H H 8.335 . 1 10 467 5 ILE N N 124.384 . 1 11 468 6 ILE H H 8.204 . 1 12 468 6 ILE N N 118.479 . 1 13 469 7 LYS H H 8.595 . 1 14 469 7 LYS N N 119.302 . 1 15 470 8 SER H H 8.162 . 1 16 470 8 SER N N 111.766 . 1 17 471 9 SER H H 7.529 . 1 18 471 9 SER N N 117.943 . 1 19 472 10 ARG H H 9.338 . 1 20 472 10 ARG N N 118.011 . 1 21 473 11 LEU H H 6.936 . 1 22 473 11 LEU N N 117.884 . 1 23 474 12 GLU H H 9.227 . 1 24 474 12 GLU N N 119.961 . 1 25 475 13 GLU H H 9.132 . 1 26 475 13 GLU N N 123.238 . 1 27 476 14 ASP H H 9.111 . 1 28 476 14 ASP N N 116.704 . 1 29 477 15 ARG H H 7.383 . 1 30 477 15 ARG N N 121.479 . 1 31 478 16 LYS H H 8.325 . 1 32 478 16 LYS N N 119.187 . 1 33 479 17 ARG H H 8.157 . 1 34 479 17 ARG N N 117.047 . 1 35 480 18 TYR H H 7.934 . 1 36 480 18 TYR N N 120.969 . 1 37 481 19 LEU H H 8.556 . 1 38 481 19 LEU N N 119.137 . 1 39 482 20 MET H H 8.381 . 1 40 482 20 MET N N 117.261 . 1 41 483 21 THR H H 7.991 . 1 42 483 21 THR N N 115.676 . 1 43 484 22 LEU H H 7.936 . 1 44 484 22 LEU N N 122.157 . 1 45 485 23 LEU H H 8.025 . 1 46 485 23 LEU N N 118.525 . 1 47 486 24 ASP H H 7.486 . 1 48 486 24 ASP N N 117.162 . 1 49 487 25 ASP H H 7.538 . 1 50 487 25 ASP N N 118.593 . 1 51 488 26 ILE H H 7.550 . 1 52 488 26 ILE N N 121.728 . 1 53 489 27 LYS H H 9.443 . 1 54 489 27 LYS N N 127.933 . 1 55 490 28 GLY H H 8.763 . 1 56 490 28 GLY N N 112.504 . 1 57 491 29 ALA H H 7.932 . 1 58 491 29 ALA N N 125.886 . 1 59 492 30 ASN H H 8.375 . 1 60 492 30 ASN N N 118.503 . 1 61 493 31 ASP H H 8.901 . 1 62 493 31 ASP N N 123.117 . 1 63 494 32 LEU H H 8.239 . 1 64 494 32 LEU N N 122.294 . 1 65 495 33 ALA H H 8.018 . 1 66 495 33 ALA N N 121.358 . 1 67 496 34 LYS H H 7.974 . 1 68 496 34 LYS N N 119.384 . 1 69 497 35 PHE H H 8.772 . 1 70 497 35 PHE N N 121.439 . 1 71 498 36 HIS H H 8.827 . 1 72 498 36 HIS N N 118.260 . 1 73 499 37 GLN H H 8.144 . 1 74 499 37 GLN N N 116.291 . 1 75 500 38 MET H H 7.940 . 1 76 500 38 MET N N 117.445 . 1 77 501 39 LEU H H 8.866 . 1 78 501 39 LEU N N 120.987 . 1 79 502 40 VAL H H 8.196 . 1 80 502 40 VAL N N 116.383 . 1 81 503 41 LYS H H 7.160 . 1 82 503 41 LYS N N 118.718 . 1 83 504 42 ILE H H 8.052 . 1 84 504 42 ILE N N 120.232 . 1 85 505 43 ILE H H 8.502 . 1 86 505 43 ILE N N 119.924 . 1 stop_ save_