data_16259 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Beta7 Integrin Cytoplasmic Tail 1H and 15N Chemical Shift Assignments ; _BMRB_accession_number 16259 _BMRB_flat_file_name bmr16259.str _Entry_type original _Submission_date 2009-04-17 _Accession_date 2009-04-17 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details ; 1H and 15N chemical shift assignments at pH 4.5 and backbone 1H and 15N chemical shift assignments at pH 6.1 for the cytoplasmic tail of the beta7 integrin. ; loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Memo Massimiliano . . 2 Anthis Nicholas J. . 3 Campbell Iain D. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 2 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 331 "15N chemical shifts" 99 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2010-01-21 update BMRB 'complete entry citation' 2009-12-11 original author 'original release' stop_ loop_ _Related_BMRB_accession_number _Relationship 15552 'Assigments of related molecules.' 16158 'Assigments of related molecules.' 16159 'Assigments of related molecules.' 16162 'Assigments of related molecules.' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Beta integrin tyrosine phosphorylation is a conserved mechanism for regulating talin-induced integrin activation.' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 19843520 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Anthis Nicholas J. . 2 Haling Jacob R. . 3 Oxley Camilla L. . 4 Memo Massimiliano . . 5 Wegener Kate L. . 6 Lim Chinten J. . 7 Ginsberg Mark H. . 8 Campbell Iain D. . stop_ _Journal_abbreviation 'J. Biol. Chem.' _Journal_name_full 'The Journal of biological chemistry' _Journal_volume 284 _Journal_issue 52 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 36700 _Page_last 36710 _Year 2009 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'beta7 integrin tail' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'beta7 integrain tail' $beta7_integrin stop_ _System_molecular_weight 6446 _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details 'beta7 integrin tail' save_ ######################## # Monomeric polymers # ######################## save_beta7_integrin _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common beta7_integrin _Molecular_mass 6446 _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 54 _Mol_residue_sequence ; GPRLSVEIYDRREYSRFEKE QQQLNWKQDSNPLYKSAITT TINPRFQEADSPTL ; loop_ _Residue_seq_code _Residue_label 1 GLY 2 PRO 3 ARG 4 LEU 5 SER 6 VAL 7 GLU 8 ILE 9 TYR 10 ASP 11 ARG 12 ARG 13 GLU 14 TYR 15 SER 16 ARG 17 PHE 18 GLU 19 LYS 20 GLU 21 GLN 22 GLN 23 GLN 24 LEU 25 ASN 26 TRP 27 LYS 28 GLN 29 ASP 30 SER 31 ASN 32 PRO 33 LEU 34 TYR 35 LYS 36 SER 37 ALA 38 ILE 39 THR 40 THR 41 THR 42 ILE 43 ASN 44 PRO 45 ARG 46 PHE 47 GLN 48 GLU 49 ALA 50 ASP 51 SER 52 PRO 53 THR 54 LEU stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-07-08 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 2BRQ "Crystal Structure Of The Filamin A Repeat 21 Complexed With The Integrin Beta7 Cytoplasmic Tail Peptide" 57.41 31 100.00 100.00 9.89e-13 DBJ BAG10580 "integrin beta-7 precursor [synthetic construct]" 96.30 798 100.00 100.00 8.85e-28 DBJ BAH12742 "unnamed protein product [Homo sapiens]" 96.30 626 100.00 100.00 1.77e-28 GB AAA36118 "integrin beta 7 subunit [Homo sapiens]" 96.30 798 100.00 100.00 8.85e-28 GB AAA59184 "integrin beta-7 subunit [Homo sapiens]" 96.30 798 100.00 100.00 8.85e-28 GB AAA59185 "integrin beta-7 subunit [Homo sapiens]" 96.30 798 100.00 100.00 8.85e-28 GB AAB21332 "integrin beta 7 subunit [Homo sapiens]" 96.30 798 100.00 100.00 8.85e-28 GB AAB23688 "integrin beta 7 subunit [Homo sapiens]" 96.30 798 100.00 100.00 8.85e-28 REF NP_000880 "integrin beta-7 precursor [Homo sapiens]" 96.30 798 100.00 100.00 8.85e-28 REF XP_001087864 "PREDICTED: integrin beta-7 [Macaca mulatta]" 96.30 673 98.08 98.08 1.38e-27 REF XP_001141622 "PREDICTED: integrin beta-7 [Pan troglodytes]" 96.30 798 100.00 100.00 7.72e-28 REF XP_003831078 "PREDICTED: integrin beta-7 isoform X1 [Pan paniscus]" 96.30 798 100.00 100.00 7.72e-28 REF XP_004053261 "PREDICTED: integrin beta-7 isoform 1 [Gorilla gorilla gorilla]" 96.30 798 100.00 100.00 8.35e-28 SP P26010 "RecName: Full=Integrin beta-7; AltName: Full=Gut homing receptor beta subunit; Flags: Precursor" 96.30 798 100.00 100.00 8.85e-28 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species _Gene_mnemonic $beta7_integrin Human 9606 Eukaryota Metazoa Homo sapiens ITGB7 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $beta7_integrin 'recombinant technology' . Escherichia coli BL21 pET16b stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_pH_6.1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling D2O 5 % [U-2H] 'sodium phosphate' 50 mM 'natural abundance' 'sodium chloride' 100 mM 'natural abundance' DTT 1 mM 'natural abundance' DSS 0.1 mM 'natural abundance' $beta7_integrin 0.05 mM [U-15N] H2O 95 % 'natural abundance' stop_ save_ save_pH_4.5 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling D2O 5 % [U-2H] 'sodium acetate' 20 mM 'natural abundance' 'sodium azide' 0.02 % 'natural abundance' DSS 0.1 mM 'natural abundance' $beta7_integrin 0.2 mM [U-15N] H2O 95 % 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ save_SPARKY _Saveframe_category software _Name SPARKY _Version . loop_ _Vendor _Address _Electronic_address Goddard . . stop_ loop_ _Task 'data analysis' stop_ _Details . save_ save_Omega_Spectrometer_Operating_Software _Saveframe_category software _Name 'Omega Spectrometer Operating Software' _Version 'Beta 6.0.3b2' loop_ _Vendor _Address _Electronic_address GE . . stop_ loop_ _Task collection stop_ _Details . save_ save_CcpNmr_Analysis _Saveframe_category software _Name ANALYSIS _Version . loop_ _Vendor _Address _Electronic_address 'Boucher and Stevens' . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer GE _Model OMEGA _Field_strength 600 _Details . save_ save_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer GE _Model OMEGA _Field_strength 950 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $pH_6.1 save_ save_3D_1H-15N_NOESY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-15N NOESY' _Sample_label $pH_4.5 save_ save_3D_1H-15N_TOCSY_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-15N TOCSY' _Sample_label $pH_4.5 save_ save_2D_1H-15N_HSQC_4 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $pH_4.5 save_ ####################### # Sample conditions # ####################### save_pH6.1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 200 . mM pH 6.1 . pH pressure 1 . atm temperature 298 . K stop_ save_ save_pH4.5 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 10 . mM pH 4.5 . pH pressure 1 . atm temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 DSS N 15 'methyl protons' ppm 0.00 na indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_pH-4.5 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '3D 1H-15N NOESY' '3D 1H-15N TOCSY' '2D 1H-15N HSQC' stop_ loop_ _Sample_label $pH_4.5 stop_ _Sample_conditions_label $pH4.5 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'beta7 integrain tail' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 746 2 PRO HA H 4.475 0.000 1 2 747 3 ARG H H 8.516 0.002 1 3 747 3 ARG HA H 4.322 0.002 1 4 747 3 ARG HB2 H 1.787 0.000 1 5 747 3 ARG HB3 H 1.798 0.011 1 6 747 3 ARG HD2 H 3.209 0.000 1 7 747 3 ARG HD3 H 3.209 0.000 1 8 747 3 ARG HG2 H 1.657 0.000 1 9 747 3 ARG HG3 H 1.657 0.000 1 10 747 3 ARG N N 121.951 0.021 1 11 748 4 LEU H H 8.398 0.003 1 12 748 4 LEU HA H 4.399 0.000 1 13 748 4 LEU HB2 H 1.629 0.000 1 14 748 4 LEU HB3 H 1.614 0.015 1 15 748 4 LEU HD1 H 0.873 0.000 1 16 748 4 LEU HD2 H 0.873 0.000 1 17 748 4 LEU HG H 1.609 0.000 1 18 748 4 LEU N N 124.689 0.018 1 19 749 5 SER H H 8.338 0.002 1 20 749 5 SER HA H 4.468 0.001 1 21 749 5 SER HB2 H 3.860 0.000 1 22 749 5 SER HB3 H 3.860 0.001 1 23 749 5 SER N N 117.161 0.014 1 24 750 6 VAL H H 8.076 0.003 1 25 750 6 VAL HA H 4.146 0.000 1 26 750 6 VAL HB H 2.090 0.002 1 27 750 6 VAL HG1 H 0.916 0.000 1 28 750 6 VAL HG2 H 0.916 0.000 1 29 750 6 VAL N N 121.051 0.022 1 30 751 7 GLU H H 8.386 0.002 1 31 751 7 GLU HA H 4.282 0.001 1 32 751 7 GLU HB2 H 1.890 0.000 1 33 751 7 GLU HB3 H 1.900 0.010 1 34 751 7 GLU HG2 H 2.245 0.001 1 35 751 7 GLU HG3 H 2.244 0.000 1 36 751 7 GLU N N 124.078 0.024 1 37 752 8 ILE H H 8.056 0.001 1 38 752 8 ILE HA H 4.065 0.000 1 39 752 8 ILE HB H 1.763 0.000 1 40 752 8 ILE HG12 H 1.089 0.000 2 41 752 8 ILE HG13 H 1.319 0.001 2 42 752 8 ILE HG2 H 0.787 0.002 1 43 752 8 ILE N N 121.458 0.028 1 44 753 9 TYR H H 8.096 0.002 1 45 753 9 TYR HA H 4.573 0.002 1 46 753 9 TYR HB2 H 2.902 0.001 2 47 753 9 TYR HB3 H 3.045 0.001 2 48 753 9 TYR N N 123.510 0.018 1 49 754 10 ASP H H 8.238 0.002 1 50 754 10 ASP HA H 4.593 0.000 1 51 754 10 ASP HB2 H 2.644 0.003 2 52 754 10 ASP HB3 H 2.766 0.006 2 53 754 10 ASP N N 122.145 0.067 1 54 755 11 ARG H H 8.264 0.002 1 55 755 11 ARG HA H 4.218 0.004 1 56 755 11 ARG HB2 H 1.763 0.006 2 57 755 11 ARG HB3 H 1.904 0.001 2 58 755 11 ARG HG2 H 1.619 0.001 1 59 755 11 ARG HG3 H 1.620 0.000 1 60 755 11 ARG N N 122.291 0.026 1 61 756 12 ARG H H 8.317 0.002 1 62 756 12 ARG HA H 4.178 0.000 1 63 756 12 ARG HB2 H 1.782 0.000 1 64 756 12 ARG HB3 H 1.782 0.000 1 65 756 12 ARG HD2 H 3.156 0.000 1 66 756 12 ARG HD3 H 3.156 0.000 1 67 756 12 ARG HG2 H 1.581 0.009 1 68 756 12 ARG HG3 H 1.573 0.000 1 69 756 12 ARG N N 121.328 0.029 1 70 757 13 GLU H H 8.303 0.002 1 71 757 13 GLU HA H 4.248 0.001 1 72 757 13 GLU HB2 H 1.917 0.006 2 73 757 13 GLU HB3 H 2.015 0.001 2 74 757 13 GLU HG2 H 2.221 0.001 1 75 757 13 GLU HG3 H 2.220 0.000 1 76 757 13 GLU N N 120.544 0.021 1 77 758 14 TYR H H 8.145 0.002 1 78 758 14 TYR HA H 4.525 0.000 1 79 758 14 TYR HB2 H 2.951 0.000 2 80 758 14 TYR HB3 H 3.082 0.001 2 81 758 14 TYR N N 120.951 0.028 1 82 759 15 SER H H 8.271 0.002 1 83 759 15 SER HA H 4.378 0.004 1 84 759 15 SER HB2 H 3.922 0.000 1 85 759 15 SER HB3 H 3.922 0.000 1 86 759 15 SER N N 116.848 0.024 1 87 760 16 ARG H H 8.194 0.001 1 88 760 16 ARG HA H 4.153 0.001 1 89 760 16 ARG HB2 H 1.706 0.000 1 90 760 16 ARG HB3 H 1.708 0.002 1 91 760 16 ARG HD2 H 3.080 0.000 1 92 760 16 ARG HD3 H 3.080 0.000 1 93 760 16 ARG HG2 H 1.429 0.002 1 94 760 16 ARG HG3 H 1.431 0.000 1 95 760 16 ARG N N 122.411 0.021 1 96 761 17 PHE H H 8.078 0.001 1 97 761 17 PHE HA H 4.540 0.004 1 98 761 17 PHE HB2 H 2.998 0.001 2 99 761 17 PHE HB3 H 3.149 0.001 2 100 761 17 PHE N N 119.792 0.021 1 101 762 18 GLU H H 8.136 0.001 1 102 762 18 GLU HA H 4.131 0.001 1 103 762 18 GLU HB2 H 2.008 0.000 1 104 762 18 GLU HB3 H 2.010 0.004 1 105 762 18 GLU HG2 H 2.304 0.001 1 106 762 18 GLU HG3 H 2.303 0.000 1 107 762 18 GLU N N 121.005 0.023 1 108 763 19 LYS H H 8.179 0.002 1 109 763 19 LYS HA H 4.137 0.000 1 110 763 19 LYS HB2 H 1.811 0.000 1 111 763 19 LYS HB3 H 1.816 0.004 1 112 763 19 LYS HD2 H 1.469 0.000 1 113 763 19 LYS HD3 H 1.469 0.000 1 114 763 19 LYS HE2 H 2.974 0.000 1 115 763 19 LYS HE3 H 2.974 0.000 1 116 763 19 LYS HG2 H 1.411 0.002 1 117 763 19 LYS HG3 H 1.410 0.000 1 118 763 19 LYS N N 120.911 0.020 1 119 764 20 GLU H H 8.278 0.002 1 120 764 20 GLU HA H 4.177 0.003 1 121 764 20 GLU HB2 H 2.018 0.000 1 122 764 20 GLU HB3 H 2.018 0.000 1 123 764 20 GLU HG2 H 2.353 0.000 1 124 764 20 GLU HG3 H 2.353 0.000 1 125 764 20 GLU N N 120.381 0.020 1 126 765 21 GLN H H 8.174 0.001 1 127 765 21 GLN HA H 4.125 0.004 1 128 765 21 GLN HB2 H 1.949 0.000 2 129 765 21 GLN HB3 H 2.027 0.000 2 130 765 21 GLN HG2 H 2.222 0.003 1 131 765 21 GLN HG3 H 2.225 0.000 1 132 765 21 GLN N N 119.856 0.025 1 133 766 22 GLN H H 8.153 0.003 1 134 766 22 GLN HA H 4.204 0.000 1 135 766 22 GLN HB2 H 1.966 0.000 2 136 766 22 GLN HB3 H 2.073 0.000 2 137 766 22 GLN HG2 H 2.303 0.000 1 138 766 22 GLN HG3 H 2.304 0.000 1 139 766 22 GLN N N 120.167 0.014 1 140 767 23 GLN H H 8.165 0.002 1 141 767 23 GLN HA H 4.178 0.000 1 142 767 23 GLN HB2 H 1.975 0.000 2 143 767 23 GLN HB3 H 2.081 0.001 2 144 767 23 GLN HG2 H 2.324 0.002 1 145 767 23 GLN HG3 H 2.322 0.000 1 146 767 23 GLN N N 120.168 0.018 1 147 768 24 LEU H H 8.090 0.002 1 148 768 24 LEU HA H 4.245 0.000 1 149 768 24 LEU HB2 H 1.597 0.000 1 150 768 24 LEU HB3 H 1.599 0.003 1 151 768 24 LEU HD1 H 0.852 0.000 1 152 768 24 LEU HD2 H 0.852 0.000 1 153 768 24 LEU HG H 1.454 0.006 1 154 768 24 LEU N N 122.164 0.025 1 155 769 25 ASN H H 8.271 0.002 1 156 769 25 ASN HA H 4.653 0.000 1 157 769 25 ASN HB2 H 2.721 0.006 2 158 769 25 ASN HB3 H 2.808 0.003 2 159 769 25 ASN N N 118.317 0.035 1 160 770 26 TRP H H 7.927 0.004 1 161 770 26 TRP HA H 4.585 0.005 1 162 770 26 TRP HB2 H 3.270 0.000 1 163 770 26 TRP HB3 H 3.270 0.000 1 164 770 26 TRP HD1 H 7.248 0.004 1 165 770 26 TRP HE1 H 10.111 0.001 1 166 770 26 TRP HZ2 H 7.463 0.000 1 167 770 26 TRP N N 121.271 0.023 1 168 770 26 TRP NE1 N 129.427 0.013 1 169 771 27 LYS H H 7.910 0.002 1 170 771 27 LYS HA H 4.109 0.003 1 171 771 27 LYS HB2 H 1.678 0.000 1 172 771 27 LYS HB3 H 1.679 0.001 1 173 771 27 LYS HD2 H 1.539 0.000 1 174 771 27 LYS HD3 H 1.543 0.004 1 175 771 27 LYS HG2 H 1.157 0.000 1 176 771 27 LYS HG3 H 1.157 0.000 1 177 771 27 LYS N N 122.712 0.021 1 178 772 28 GLN H H 8.109 0.002 1 179 772 28 GLN HA H 4.137 0.007 1 180 772 28 GLN HB2 H 1.921 0.000 2 181 772 28 GLN HB3 H 2.028 0.005 2 182 772 28 GLN HG2 H 2.286 0.001 1 183 772 28 GLN HG3 H 2.286 0.000 1 184 772 28 GLN N N 121.037 0.020 1 185 773 29 ASP H H 8.310 0.003 1 186 773 29 ASP HA H 4.568 0.001 1 187 773 29 ASP HB2 H 2.675 0.000 1 188 773 29 ASP HB3 H 2.681 0.005 1 189 773 29 ASP N N 121.030 0.035 1 190 774 30 SER H H 8.137 0.001 1 191 774 30 SER HA H 4.390 0.000 1 192 774 30 SER HB2 H 3.835 0.000 1 193 774 30 SER HB3 H 3.833 0.002 1 194 774 30 SER N N 115.587 0.024 1 195 775 31 ASN H H 8.326 0.003 1 196 775 31 ASN HA H 4.920 0.000 1 197 775 31 ASN HB2 H 2.712 0.000 2 198 775 31 ASN HB3 H 2.856 0.000 2 199 775 31 ASN N N 121.643 0.023 1 200 776 32 PRO HA H 4.367 0.000 1 201 776 32 PRO HB2 H 1.937 0.000 1 202 776 32 PRO HB3 H 1.937 0.000 1 203 776 32 PRO HD2 H 3.721 0.000 1 204 776 32 PRO HD3 H 3.721 0.000 1 205 776 32 PRO HG2 H 1.877 0.000 1 206 776 32 PRO HG3 H 1.877 0.000 1 207 777 33 LEU H H 8.104 0.001 1 208 777 33 LEU HA H 4.196 0.001 1 209 777 33 LEU HB2 H 1.495 0.000 1 210 777 33 LEU HB3 H 1.496 0.001 1 211 777 33 LEU HD1 H 0.895 0.000 1 212 777 33 LEU HD2 H 0.895 0.000 1 213 777 33 LEU HG H 1.372 0.002 1 214 777 33 LEU N N 120.069 0.050 1 215 778 34 TYR H H 7.784 0.001 1 216 778 34 TYR HA H 4.552 0.001 1 217 778 34 TYR HB2 H 2.949 0.003 2 218 778 34 TYR HB3 H 3.089 0.004 2 219 778 34 TYR N N 119.528 0.020 1 220 779 35 LYS H H 7.975 0.002 1 221 779 35 LYS HA H 4.261 0.000 1 222 779 35 LYS HB2 H 1.793 0.000 1 223 779 35 LYS HB3 H 1.790 0.003 1 224 779 35 LYS HD2 H 1.688 0.000 1 225 779 35 LYS HD3 H 1.687 0.000 1 226 779 35 LYS HG2 H 1.377 0.020 1 227 779 35 LYS HG3 H 1.357 0.000 1 228 779 35 LYS N N 122.844 0.015 1 229 780 36 SER H H 8.169 0.002 1 230 780 36 SER HA H 4.382 0.003 1 231 780 36 SER HB2 H 3.867 0.000 1 232 780 36 SER HB3 H 3.866 0.001 1 233 780 36 SER N N 116.800 0.020 1 234 781 37 ALA H H 8.309 0.003 1 235 781 37 ALA HA H 4.372 0.003 1 236 781 37 ALA HB H 1.392 0.000 1 237 781 37 ALA N N 126.043 0.021 1 238 782 38 ILE H H 8.094 0.002 1 239 782 38 ILE HA H 4.221 0.001 1 240 782 38 ILE HB H 1.876 0.001 1 241 782 38 ILE HG12 H 1.193 0.001 2 242 782 38 ILE HG13 H 1.481 0.001 2 243 782 38 ILE HG2 H 0.907 0.004 1 244 782 38 ILE N N 119.695 0.018 1 245 783 39 THR H H 8.217 0.002 1 246 783 39 THR HA H 4.447 0.000 1 247 783 39 THR HB H 4.251 0.011 1 248 783 39 THR HG2 H 1.210 0.004 1 249 783 39 THR N N 118.178 0.027 1 250 784 40 THR H H 8.156 0.004 1 251 784 40 THR HA H 4.420 0.004 1 252 784 40 THR HB H 4.248 0.000 1 253 784 40 THR HG2 H 1.205 0.002 1 254 784 40 THR N N 116.621 0.031 1 255 785 41 THR H H 8.163 0.001 1 256 785 41 THR HA H 4.339 0.009 1 257 785 41 THR HB H 4.162 0.004 1 258 785 41 THR HG2 H 1.188 0.002 1 259 785 41 THR N N 117.102 0.029 1 260 786 42 ILE H H 8.169 0.004 1 261 786 42 ILE HA H 4.157 0.000 1 262 786 42 ILE HB H 1.822 0.002 1 263 786 42 ILE HG12 H 1.171 0.001 2 264 786 42 ILE HG13 H 1.441 0.000 2 265 786 42 ILE HG2 H 0.853 0.003 1 266 786 42 ILE N N 123.468 0.016 1 267 787 43 ASN H H 8.513 0.005 1 268 787 43 ASN HA H 4.951 0.000 1 269 787 43 ASN HB2 H 2.684 0.000 2 270 787 43 ASN HB3 H 2.831 0.000 2 271 787 43 ASN N N 124.192 0.019 1 272 788 44 PRO HA H 4.368 0.000 1 273 788 44 PRO HB2 H 2.006 0.000 1 274 788 44 PRO HB3 H 2.006 0.000 1 275 788 44 PRO HD2 H 3.736 0.000 1 276 788 44 PRO HD3 H 3.736 0.000 1 277 788 44 PRO HG2 H 1.868 0.000 1 278 788 44 PRO HG3 H 1.868 0.000 1 279 789 45 ARG H H 8.197 0.001 1 280 789 45 ARG HA H 4.218 0.005 1 281 789 45 ARG HB2 H 1.658 0.000 1 282 789 45 ARG HB3 H 1.664 0.005 1 283 789 45 ARG HG2 H 1.506 0.000 1 284 789 45 ARG HG3 H 1.506 0.000 1 285 789 45 ARG N N 119.733 0.015 1 286 790 46 PHE H H 8.048 0.002 1 287 790 46 PHE HA H 4.621 0.000 1 288 790 46 PHE HB2 H 3.029 0.006 2 289 790 46 PHE HB3 H 3.160 0.003 2 290 790 46 PHE N N 120.535 0.019 1 291 791 47 GLN H H 8.160 0.001 1 292 791 47 GLN HA H 4.278 0.003 1 293 791 47 GLN HB2 H 1.923 0.004 2 294 791 47 GLN HB3 H 2.044 0.003 2 295 791 47 GLN HG2 H 2.266 0.000 1 296 791 47 GLN HG3 H 2.266 0.000 1 297 791 47 GLN N N 121.856 0.023 1 298 792 48 GLU H H 8.332 0.002 1 299 792 48 GLU HA H 4.264 0.001 1 300 792 48 GLU HB2 H 1.961 0.002 2 301 792 48 GLU HB3 H 2.085 0.002 2 302 792 48 GLU HG2 H 2.379 0.000 1 303 792 48 GLU HG3 H 2.379 0.000 1 304 792 48 GLU N N 122.173 0.020 1 305 793 49 ALA H H 8.360 0.002 1 306 793 49 ALA HA H 4.295 0.005 1 307 793 49 ALA HB H 1.389 0.002 1 308 793 49 ALA N N 124.929 0.021 1 309 794 50 ASP H H 8.314 0.003 1 310 794 50 ASP HA H 4.633 0.001 1 311 794 50 ASP HB2 H 2.704 0.000 1 312 794 50 ASP HB3 H 2.730 0.000 2 313 794 50 ASP N N 118.879 0.021 1 314 795 51 SER H H 8.116 0.002 1 315 795 51 SER HA H 4.747 0.000 1 316 795 51 SER HB2 H 3.795 0.000 2 317 795 51 SER HB3 H 3.845 0.000 2 318 795 51 SER N N 116.858 0.012 1 319 796 52 PRO HA H 4.516 0.000 1 320 797 53 THR H H 8.253 0.002 1 321 797 53 THR HA H 4.310 0.000 1 322 797 53 THR HB H 4.203 0.000 1 323 797 53 THR HG2 H 1.205 0.000 1 324 797 53 THR N N 115.274 0.027 1 325 798 54 LEU H H 7.955 0.002 1 326 798 54 LEU HA H 4.219 0.000 1 327 798 54 LEU HB2 H 1.608 0.000 1 328 798 54 LEU HB3 H 1.608 0.000 1 329 798 54 LEU HD1 H 0.876 0.000 1 330 798 54 LEU HD2 H 0.876 0.000 1 331 798 54 LEU HG H 1.481 0.000 1 332 798 54 LEU N N 130.305 0.062 1 stop_ save_ save_pH-6.1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $pH_6.1 stop_ _Sample_conditions_label $pH6.1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'beta7 integrain tail' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 747 3 ARG H H 8.520 . 1 2 747 3 ARG N N 122.042 . 1 3 748 4 LEU H H 8.404 . 1 4 748 4 LEU N N 124.619 . 1 5 749 5 SER H H 8.328 . 1 6 749 5 SER N N 117.128 . 1 7 750 6 VAL H H 8.072 . 1 8 750 6 VAL N N 120.972 . 1 9 751 7 GLU H H 8.406 . 1 10 751 7 GLU N N 124.147 . 1 11 752 8 ILE H H 8.047 . 1 12 752 8 ILE N N 121.328 . 1 13 753 9 TYR H H 8.091 . 1 14 753 9 TYR N N 123.624 . 1 15 754 10 ASP H H 8.239 . 1 16 754 10 ASP N N 122.269 . 1 17 755 11 ARG H H 8.251 . 1 18 755 11 ARG N N 122.321 . 1 19 756 12 ARG H H 8.322 . 1 20 756 12 ARG N N 121.623 . 1 21 757 13 GLU H H 8.357 . 1 22 757 13 GLU N N 120.984 . 1 23 758 14 TYR H H 8.149 . 1 24 758 14 TYR N N 121.045 . 1 25 759 15 SER H H 8.342 . 1 26 759 15 SER N N 117.204 . 1 27 760 16 ARG H H 8.241 . 1 28 760 16 ARG N N 122.613 . 1 29 761 17 PHE H H 8.092 . 1 30 761 17 PHE N N 119.853 . 1 31 762 18 GLU H H 8.162 . 1 32 762 18 GLU N N 121.265 . 1 33 763 19 LYS H H 8.184 . 1 34 763 19 LYS N N 120.898 . 1 35 764 20 GLU H H 8.322 . 1 36 764 20 GLU N N 120.596 . 1 37 765 21 GLN H H 8.165 . 1 38 765 21 GLN N N 119.773 . 1 39 766 22 GLN H H 8.156 . 1 40 766 22 GLN N N 120.180 . 1 41 767 23 GLN H H 8.151 . 1 42 767 23 GLN N N 120.118 . 1 43 768 24 LEU H H 8.080 . 1 44 768 24 LEU N N 122.184 . 1 45 769 25 ASN H H 8.271 . 1 46 769 25 ASN N N 118.438 . 1 47 770 26 TRP H H 7.928 . 1 48 770 26 TRP N N 121.306 . 1 49 771 27 LYS H H 7.916 . 1 50 771 27 LYS N N 122.778 . 1 51 772 28 GLN H H 8.110 . 1 52 772 28 GLN N N 121.107 . 1 53 773 29 ASP H H 8.310 . 1 54 773 29 ASP N N 121.239 . 1 55 774 30 SER H H 8.126 . 1 56 774 30 SER N N 115.530 . 1 57 775 31 ASN H H 8.345 . 1 58 775 31 ASN N N 121.714 . 1 59 777 33 LEU H H 8.109 . 1 60 777 33 LEU N N 120.214 . 1 61 778 34 TYR H H 7.792 . 1 62 778 34 TYR N N 119.625 . 1 63 779 35 LYS H H 7.983 . 1 64 779 35 LYS N N 122.903 . 1 65 780 36 SER H H 8.174 . 1 66 780 36 SER N N 116.849 . 1 67 781 37 ALA H H 8.311 . 1 68 781 37 ALA N N 126.101 . 1 69 782 38 ILE H H 8.095 . 1 70 782 38 ILE N N 119.751 . 1 71 783 39 THR H H 8.223 . 1 72 783 39 THR N N 118.318 . 1 73 784 40 THR H H 8.166 . 1 74 784 40 THR N N 116.767 . 1 75 785 41 THR H H 8.176 . 1 76 785 41 THR N N 117.315 . 1 77 786 42 ILE H H 8.176 . 1 78 786 42 ILE N N 123.609 . 1 79 787 43 ASN H H 8.525 . 1 80 787 43 ASN N N 124.321 . 1 81 789 45 ARG H H 8.221 . 1 82 789 45 ARG N N 119.977 . 1 83 790 46 PHE H H 8.082 . 1 84 790 46 PHE N N 120.736 . 1 85 791 47 GLN H H 8.170 . 1 86 791 47 GLN N N 122.029 . 1 87 792 48 GLU H H 8.385 . 1 88 792 48 GLU N N 122.650 . 1 89 793 49 ALA H H 8.348 . 1 90 793 49 ALA N N 124.810 . 1 91 794 50 ASP H H 8.299 . 1 92 794 50 ASP N N 119.483 . 1 93 795 51 SER H H 8.130 . 1 94 795 51 SER N N 116.895 . 1 95 797 53 THR H H 8.280 . 1 96 797 53 THR N N 115.468 . 1 97 798 54 LEU H H 7.918 . 1 98 798 54 LEU N N 130.937 . 1 stop_ save_