data_16274 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; SLBP PAPA peptide ; _BMRB_accession_number 16274 _BMRB_flat_file_name bmr16274.str _Entry_type original _Submission_date 2009-05-04 _Accession_date 2009-05-04 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details 'Assignments for a 20-residue peptide from the RNA binding domain of SLBP that is phosphorylated and undergoes prolyl isomerization.' loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Thapar Roopa . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 98 "15N chemical shifts" 17 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2014-05-16 update BMRB 'update entry citation' 2012-08-06 original author 'original release' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'The prolyl isomerase Pin1 targets stem-loop binding protein (SLBP) to dissociate the SLBP-histone mRNA complex linking histone mRNA decay with SLBP ubiquitination.' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 22907757 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Krishnan Nithya . . 2 Lam Tukiet T. . 3 Fritz Andrew . . 4 Rempinski Donald . . 5 Minderman Kieran . . 6 Berezney Hans . . 7 Marzluff Ronald . . 8 Thapar William F. . stop_ _Journal_abbreviation 'Mol. Cell. Biol.' _Journal_name_full 'Molecular and cellular biology' _Journal_volume 32 _Journal_issue 21 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 4306 _Page_last 4322 _Year 2012 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name SLBP _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label SLBP $SLBP stop_ _System_molecular_weight 2432.8 _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . loop_ _Biological_function 'RNA binding' stop_ _Database_query_date . _Details 'peptide from the RNA binding domain of Stem-Loop Binding Protein' save_ ######################## # Monomeric polymers # ######################## save_SLBP _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common SLBP _Molecular_mass . _Mol_thiol_state 'not present' loop_ _Biological_function 'Histone mRNA binding and processing. Protein is required for processing, export, and translation of replication-dependent histone mRNAs' stop_ _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 21 _Mol_residue_sequence ; HLRQPGIHAAKTANKFKKYS R ; loop_ _Residue_seq_code _Residue_label 1 HIS 2 LEU 3 ARG 4 GLN 5 PRO 6 GLY 7 ILE 8 HIS 9 ALA 10 ALA 11 LYS 12 THR 13 ALA 14 ASN 15 LYS 16 PHE 17 LYS 18 LYS 19 TYR 20 SER 21 ARG stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value GB NP_006518 SLBP . . . . . stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species _Gene_mnemonic $SLBP Human 9606 Eukaryota Metazoa Homo sapiens 'Stem-Loop Binding Protein' stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name _Details $SLBP 'chemical synthesis' . . . . . 'Peptide was synthesized and not expressed' stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details '20 mM Tris acetate, 50 mM NaCl, 90% H2O' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $SLBP 5 mM 'natural abundance' H2O 90 % 'natural abundance' D2O 10 % 'natural abundance' 'Tris acetate' 20 mM 'natural abundance' NaCl 50 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_Felix _Saveframe_category software _Name FELIX _Version . loop_ _Vendor _Address _Electronic_address 'Accelrys Software Inc.' . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model INOVA _Field_strength 700 _Details . save_ save_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model INOVA _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-1H_TOCSY_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H TOCSY' _Sample_label $sample_1 save_ save_2D_DQF-COSY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D DQF-COSY' _Sample_label $sample_1 save_ save_2D_1H-1H_NOESY_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_1 save_ save_2D_1H-15N_HSQC_4 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details ; 20 mM Tris acetate buffer, 50 mM NaCl, 90% H2O, pH 5 ; loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 50 . mM pH 5 0.05 pH pressure 1 . atm temperature 278 0.2 K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS H 1 'methyl protons' ppm 0.00 external direct . . . 1.00 DSS N 15 'methyl protons' ppm 0.00 internal indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Software_label $Felix stop_ loop_ _Experiment_label '2D 1H-1H TOCSY' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name SLBP _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 1 1 HIS H H 8.02 0.02 1 2 1 1 HIS N N 121.55 0.05 1 3 2 2 LEU H H 8.68 0.02 1 4 2 2 LEU HA H 4.19 0.02 1 5 2 2 LEU HB2 H 1.41 0.02 1 6 2 2 LEU HB3 H 1.41 0.02 1 7 2 2 LEU HD1 H 0.73 0.02 1 8 2 2 LEU HD2 H 0.69 0.02 1 9 2 2 LEU HG H 1.37 0.02 1 10 2 2 LEU N N 126.66 0.05 1 11 3 3 ARG H H 8.54 0.02 1 12 3 3 ARG HA H 4.13 0.02 1 13 3 3 ARG HB2 H 1.60 0.02 1 14 3 3 ARG HB3 H 1.60 0.02 1 15 3 3 ARG HD2 H 3.00 0.02 1 16 3 3 ARG HD3 H 3.00 0.02 1 17 3 3 ARG HG2 H 1.47 0.02 1 18 3 3 ARG HG3 H 1.41 0.02 1 19 3 3 ARG N N 125.21 0.05 1 20 4 4 GLN H H 8.47 0.02 1 21 4 4 GLN HA H 4.43 0.02 1 22 4 4 GLN HB2 H 1.89 0.02 1 23 4 4 GLN HB3 H 1.74 0.02 1 24 4 4 GLN HG2 H 2.19 0.02 1 25 4 4 GLN HG3 H 2.19 0.02 1 26 4 4 GLN N N 125.04 0.05 1 27 6 6 GLY H H 8.38 0.02 1 28 6 6 GLY HA2 H 3.76 0.02 1 29 6 6 GLY HA3 H 3.72 0.02 1 30 6 6 GLY N N 110.27 0.05 1 31 7 7 ILE H H 7.87 0.02 1 32 7 7 ILE HA H 3.91 0.02 1 33 7 7 ILE HB H 1.60 0.02 1 34 7 7 ILE HD1 H 0.62 0.02 1 35 7 7 ILE HG12 H 1.11 0.02 1 36 7 7 ILE HG13 H 0.93 0.02 1 37 7 7 ILE N N 121.08 0.05 1 38 8 8 HIS H H 8.55 0.02 1 39 8 8 HIS HA H 4.55 0.02 1 40 8 8 HIS HB2 H 3.06 0.02 1 41 8 8 HIS HB3 H 2.95 0.02 1 42 8 8 HIS N N 124.05 0.05 1 43 9 9 ALA H H 8.29 0.02 1 44 9 9 ALA HA H 4.11 0.02 1 45 9 9 ALA HB H 1.18 0.02 1 46 9 9 ALA N N 127.05 0.05 1 47 10 10 ALA H H 8.25 0.02 1 48 10 10 ALA HA H 4.10 0.02 1 49 10 10 ALA HB H 1.20 0.02 1 50 11 11 LYS H H 8.41 0.02 1 51 11 11 LYS HA H 4.16 0.02 1 52 11 11 LYS HB2 H 1.65 0.02 1 53 11 11 LYS HD2 H 1.59 0.02 1 54 11 11 LYS HD3 H 1.50 0.02 1 55 11 11 LYS HE2 H 3.06 0.02 1 56 11 11 LYS HE3 H 2.80 0.02 1 57 11 11 LYS HG2 H 1.30 0.02 1 58 11 11 LYS HG3 H 1.24 0.02 1 59 12 12 THR H H 8.07 0.02 1 60 12 12 THR HA H 4.00 0.02 1 61 12 12 THR HB H 4.11 0.02 1 62 12 12 THR N N 117.13 0.05 1 63 13 13 ALA H H 8.34 0.02 1 64 13 13 ALA HA H 4.11 0.02 1 65 13 13 ALA HB H 1.19 0.02 1 66 13 13 ALA N N 127.93 0.05 1 67 14 14 ASN H H 8.20 0.02 1 68 14 14 ASN HA H 4.40 0.02 1 69 14 14 ASN HB2 H 2.86 0.02 1 70 14 14 ASN HB3 H 2.76 0.02 1 71 14 14 ASN N N 122.93 0.05 1 72 15 15 LYS H H 8.08 0.02 1 73 15 15 LYS HA H 4.09 0.02 1 74 15 15 LYS HB2 H 1.70 0.02 1 75 15 15 LYS HD2 H 1.56 0.02 1 76 15 15 LYS HD3 H 1.56 0.02 1 77 15 15 LYS HE2 H 2.99 0.02 1 78 15 15 LYS HE3 H 2.99 0.02 1 79 15 15 LYS HG2 H 1.43 0.02 1 80 15 15 LYS HG3 H 1.43 0.02 1 81 15 15 LYS N N 126.04 0.05 1 82 16 16 PHE H H 8.06 0.02 1 83 16 16 PHE HA H 4.22 0.02 1 84 16 16 PHE HB2 H 3.62 0.02 1 85 16 16 PHE HB3 H 3.62 0.02 1 86 16 16 PHE N N 124.55 0.05 1 87 17 17 LYS H H 8.13 0.02 1 88 17 17 LYS HA H 4.00 0.02 1 89 17 17 LYS HB2 H 1.47 0.02 1 90 17 17 LYS HB3 H 1.47 0.02 1 91 17 17 LYS HD2 H 1.15 0.02 1 92 17 17 LYS HD3 H 1.15 0.02 1 93 17 17 LYS HG2 H 1.06 0.02 1 94 17 17 LYS HG3 H 1.06 0.02 1 95 17 17 LYS N N 124.38 0.05 1 96 18 18 LYS H H 8.18 0.02 1 97 18 18 LYS HA H 3.99 0.02 1 98 18 18 LYS HB2 H 1.46 0.02 1 99 18 18 LYS HB3 H 1.46 0.02 1 100 18 18 LYS HD2 H 1.39 0.02 1 101 18 18 LYS HD3 H 1.39 0.02 1 102 18 18 LYS HG2 H 1.02 0.02 1 103 18 18 LYS HG3 H 1.02 0.02 1 104 18 18 LYS N N 123.25 0.05 1 105 19 19 TYR H H 8.04 0.02 1 106 19 19 TYR HA H 4.41 0.02 1 107 19 19 TYR HB2 H 2.96 0.02 1 108 19 19 TYR HB3 H 2.79 0.02 1 109 19 19 TYR N N 119.48 0.05 1 110 20 20 SER H H 8.37 0.02 1 111 20 20 SER HA H 4.15 0.02 1 112 20 20 SER HB2 H 3.77 0.02 1 113 20 20 SER HB3 H 3.73 0.02 1 114 21 21 ARG H H 7.01 0.02 1 115 21 21 ARG N N 130.05 0.05 1 stop_ save_