data_16422 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Solution Structure and Dynamics of the N-terminal Cystosolic Domain of Rhomboid Intramembrane Protease from Pseudomonas aeruginosa: Insights into a Functional Role in Intramembrane Proteolysis ; _BMRB_accession_number 16422 _BMRB_flat_file_name bmr16422.str _Entry_type original _Submission_date 2009-07-23 _Accession_date 2009-07-23 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 'Del Rio' Armando . . 2 Dutta Kaushik . . 3 Chavez Jose . . 4 Ubarretxena-Belandia Iban . . 5 Ghose Ranajeet . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count binding_constants 1 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2010-03-08 original author . stop_ _Original_release_date 2010-03-08 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Solution Structure and Dynamics of the N-terminal Cystosolic Domain of Rhomboid Intramembrane Protease from Pseudomonas aeruginosa: Insights into a Functional Role in Intramembrane Proteolysis' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 17059825 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 'Del Rio' Armando . . 2 Dutta Kaushik . . 3 Chavez Jose . . 4 Ubarretxena-Belandia Iban . . 5 Ghose Ranajeet . . stop_ _Journal_abbreviation 'J. Mol. Biol.' _Journal_volume 1 _Journal_issue 365 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 109 _Page_last 122 _Year 2007 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'C16PN/NRho complex' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label NRho $NRho C16PN $C16PN stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_NRho _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common NRho _Molecular_mass . _Mol_thiol_state 'not present' _Details 'Rhomboid Intramembrane Protease' ############################## # Polymer residue sequence # ############################## _Residue_count 70 _Mol_residue_sequence ; MSAVQVLKFPLSVDLAGFVG LLRRLNVPHRVSEESGQQVL WVPDERLAEQVRELYRRYPE GDPQATLEAA ; loop_ _Residue_seq_code _Residue_label 1 MET 2 SER 3 ALA 4 VAL 5 GLN 6 VAL 7 LEU 8 LYS 9 PHE 10 PRO 11 LEU 12 SER 13 VAL 14 ASP 15 LEU 16 ALA 17 GLY 18 PHE 19 VAL 20 GLY 21 LEU 22 LEU 23 ARG 24 ARG 25 LEU 26 ASN 27 VAL 28 PRO 29 HIS 30 ARG 31 VAL 32 SER 33 GLU 34 GLU 35 SER 36 GLY 37 GLN 38 GLN 39 VAL 40 LEU 41 TRP 42 VAL 43 PRO 44 ASP 45 GLU 46 ARG 47 LEU 48 ALA 49 GLU 50 GLN 51 VAL 52 ARG 53 GLU 54 LEU 55 TYR 56 ARG 57 ARG 58 TYR 59 PRO 60 GLU 61 GLY 62 ASP 63 PRO 64 GLN 65 ALA 66 THR 67 LEU 68 GLU 69 ALA 70 ALA stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-11-04 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 2GQC "Solution Structure Of The N-Terminal Domain Of Rhomboid Intramembrane Protease From P. Aeruginosa" 100.00 70 100.00 100.00 4.07e-41 DBJ BAK91039 "hypothetical protein NCGM2_4205 [Pseudomonas aeruginosa NCGM2.S1]" 100.00 286 100.00 100.00 3.15e-39 DBJ BAP22307 "hypothetical protein NCGM1900_3216 [Pseudomonas aeruginosa]" 100.00 286 100.00 100.00 3.15e-39 DBJ BAP49934 "hypothetical protein NCGM1984_1953 [Pseudomonas aeruginosa]" 100.00 286 100.00 100.00 3.15e-39 DBJ BAQ38821 "hypothetical protein PA257_2215 [Pseudomonas aeruginosa]" 100.00 286 100.00 100.00 3.15e-39 DBJ BAR66878 "GlpG protein [Pseudomonas aeruginosa]" 100.00 286 100.00 100.00 3.58e-39 EMBL CAW26702 "hypothetical [Pseudomonas aeruginosa LESB58]" 100.00 286 100.00 100.00 3.36e-39 EMBL CCQ87103 "GlpG protein (membrane protein of glp regulon) [Pseudomonas aeruginosa 18A]" 100.00 286 100.00 100.00 3.58e-39 EMBL CDH70226 "hypothetical protein P38_1942 [Pseudomonas aeruginosa MH38]" 100.00 286 100.00 100.00 3.58e-39 EMBL CDH76316 "hypothetical protein PAMH27_1910 [Pseudomonas aeruginosa MH27]" 100.00 286 100.00 100.00 3.15e-39 EMBL CDI91489 "rhomboid-like protein [Pseudomonas aeruginosa PA38182]" 100.00 286 100.00 100.00 3.58e-39 GB AAG06474 "hypothetical protein PA3086 [Pseudomonas aeruginosa PAO1]" 100.00 286 100.00 100.00 3.58e-39 GB ABJ12318 "hypothetical protein PA14_24240 [Pseudomonas aeruginosa UCBPP-PA14]" 100.00 286 100.00 100.00 2.58e-39 GB ABR81084 "membrane protein, putative [Pseudomonas aeruginosa PA7]" 100.00 286 100.00 100.00 2.55e-39 GB AEO74363 "hypothetical protein PAM18_1877 [Pseudomonas aeruginosa M18]" 100.00 286 100.00 100.00 3.58e-39 GB AFM64074 "rhomboid family protein [Pseudomonas aeruginosa DK2]" 100.00 286 100.00 100.00 3.58e-39 REF NP_251776 "hypothetical protein PA3086 [Pseudomonas aeruginosa PAO1]" 100.00 286 100.00 100.00 3.58e-39 REF WP_003091333 "MULTISPECIES: rhomboid family intramembrane serine protease [Pseudomonas]" 100.00 286 100.00 100.00 3.15e-39 REF WP_003104987 "MULTISPECIES: rhomboid family intramembrane serine protease [Pseudomonas]" 100.00 286 100.00 100.00 3.58e-39 REF WP_003110073 "rhomboid family intramembrane serine protease [Pseudomonas aeruginosa]" 100.00 286 98.57 98.57 1.07e-38 REF WP_003138531 "rhomboid family intramembrane serine protease [Pseudomonas aeruginosa]" 100.00 286 100.00 100.00 2.58e-39 stop_ save_ ############# # Ligands # ############# save_C16PN _Saveframe_category ligand _Mol_type non-polymer _Name_common C16PN _Molecular_mass . _Details . _Mol_thiol_state 'not present' _Sequence_homology_query_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $NRho 'Pseudomonas aeruginosa' 287 Bacteria . Pseudomonas aeruginosa stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Variant _Vector_name $NRho 'recombinant technology' . Escherichia coli B12 D3 pET15b stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $NRho 1 mM [U-15N] $C16PN 20 mM 'natural abundance' TRIS 20 mM 'natural abundance' 'sodium chloride' 50 mM 'natural abundance' H2O 100 % 'natural abundance' stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $NRho 0.8 mM [U-15N] $C16PN 20 mM 'natural abundance' TRIS 20 mM 'natural abundance' 'sodium chloride' 50 mM 'natural abundance' H2O 100 % 'natural abundance' stop_ save_ save_sample_3 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $NRho 0.6 mM [U-15N] $C16PN 20 mM 'natural abundance' TRIS 20 mM 'natural abundance' 'sodium chloride' 50 mM 'natural abundance' H2O 100 % 'natural abundance' stop_ save_ save_sample_4 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $NRho 0.5 mM [U-15N] $C16PN 20 mM 'natural abundance' TRIS 20 mM 'natural abundance' 'sodium chloride' 50 mM 'natural abundance' H2O 100 % 'natural abundance' stop_ save_ save_sample_5 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $NRho 0.4 mM [U-15N] $C16PN 20 mM 'natural abundance' TRIS 20 mM 'natural abundance' 'sodium chloride' 50 mM 'natural abundance' H2O 100 % 'natural abundance' stop_ save_ save_sample_6 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $NRho 0.3 mM [U-15N] $C16PN 20 mM 'natural abundance' TRIS 20 mM 'natural abundance' 'sodium chloride' 50 mM 'natural abundance' H2O 100 % 'natural abundance' stop_ save_ save_sample_7 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $NRho 0.2 mM [U-15N] $C16PN 20 mM 'natural abundance' TRIS 20 mM 'natural abundance' 'sodium chloride' 50 mM 'natural abundance' H2O 100 % 'natural abundance' stop_ save_ save_sample_8 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $NRho 0.1 mM [U-15N] $C16PN 20 mM 'natural abundance' TRIS 20 mM 'natural abundance' 'sodium chloride' 50 mM 'natural abundance' H2O 100 % 'natural abundance' stop_ save_ save_sample_9 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $NRho 1 mM [U-15N] TRIS 20 mM 'natural abundance' 'sodium chloride' 50 mM 'natural abundance' H2O 100 % 'natural abundance' stop_ save_ save_sample_10 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $C16PN 20 mM 'natural abundance' TRIS 20 mM 'natural abundance' 'sodium chloride' 50 mM 'natural abundance' H2O 100 % 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_NMRView _Saveframe_category software _Name NMRView _Version . loop_ _Vendor _Address _Electronic_address 'Johnson, One Moon Scientific' . . stop_ loop_ _Task 'data analysis' stop_ _Details . save_ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model INOVA _Field_strength 600 _Details . save_ save_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 700 _Details 'equipped with cryoprobe' save_ save_spectrometer_3 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 800 _Details 'equipped with cryoprobe' save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_2D_1H-15N_HSQC_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_2 save_ save_2D_1H-15N_HSQC_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_3 save_ save_2D_1H-15N_HSQC_4 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_4 save_ save_2D_1H-15N_HSQC_5 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_5 save_ save_2D_1H-15N_HSQC_6 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_6 save_ save_2D_1H-15N_HSQC_7 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_7 save_ save_2D_1H-15N_HSQC_8 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_8 save_ save_2D_1H-15N_HSQC_9 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_9 save_ save_2D_1H-15N_HSQC_10 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_10 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units temperature 298 . K pH 5.5 . pH pressure 1 . atm stop_ save_