data_16815 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Structure of the three-Cys2His2 domain of mouse testis zinc finger protein ; _BMRB_accession_number 16815 _BMRB_flat_file_name bmr16815.str _Entry_type original _Submission_date 2010-03-31 _Accession_date 2010-03-31 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Chou Chun-Chi . . 2 Lou Yuan-Chao . . 3 Chen Chinpan . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 139 "13C chemical shifts" 103 "15N chemical shifts" 25 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2010-07-27 original author . stop_ loop_ _Related_BMRB_accession_number _Relationship 16814 'mouse testis zinc finger protein' stop_ _Original_release_date 2010-07-27 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Structure and DNA binding characteristics of the three-Cys2His2 domain of mouse testis zinc finger protein.' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 20544958 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Chou Chun-Chi . . 2 Lou Yuan-Chao . . 3 Tang Tang K. . 4 Chen Chinpan . . stop_ _Journal_abbreviation Proteins _Journal_name_full Proteins _Journal_volume 78 _Journal_issue 10 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 2202 _Page_last 2212 _Year 2010 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name mTZD_finger3 _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label mTZD_finger3 $mTZD_finger3 'ZINC ION' $ZN stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_mTZD_finger3 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common mTZD_finger3 _Name_variant @ _Abbreviation_common @ _Molecular_mass 2872.3 _Mol_thiol_state 'free and other bound' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 27 _Mol_residue_sequence ; AAPYRCPLCRAGCPSLASMQ AHMRGHS ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 56 ALA 2 57 ALA 3 58 PRO 4 59 TYR 5 60 ARG 6 61 CYS 7 62 PRO 8 63 LEU 9 64 CYS 10 65 ARG 11 66 ALA 12 67 GLY 13 68 CYS 14 69 PRO 15 70 SER 16 71 LEU 17 72 ALA 18 73 SER 19 74 MET 20 75 GLN 21 76 ALA 22 77 HIS 23 78 MET 24 79 ARG 25 80 GLY 26 81 HIS 27 82 SER stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-09-30 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 2KVG "Structure Of The Three-Cys2his2 Domain Of Mouse Testis Zinc Finger Protein" 100.00 27 100.00 100.00 3.13e-09 DBJ BAB30015 "unnamed protein product [Mus musculus]" 100.00 101 100.00 100.00 3.22e-09 GB AAF61244 "repressor of GATA [Mus musculus]" 100.00 465 100.00 100.00 1.33e-08 GB AAI37688 "Zinc finger and BTB domain containing 32 [Mus musculus]" 100.00 465 100.00 100.00 1.33e-08 GB AAK13198 "zinc finger protein TZFP [Mus musculus]" 100.00 465 100.00 100.00 1.33e-08 GB EDL24005 "zinc finger and BTB domain containing 32 [Mus musculus]" 100.00 465 100.00 100.00 1.33e-08 GB EDM07732 "rCG53796 [Rattus norvegicus]" 100.00 483 100.00 100.00 1.48e-08 REF NP_001102987 "zinc finger and BTB domain-containing protein 32 [Rattus norvegicus]" 100.00 483 100.00 100.00 1.48e-08 REF NP_067372 "zinc finger and BTB domain-containing protein 32 [Mus musculus]" 100.00 465 100.00 100.00 1.33e-08 REF XP_002920975 "PREDICTED: zinc finger and BTB domain-containing protein 32 isoform X1 [Ailuropoda melanoleuca]" 100.00 488 100.00 100.00 1.29e-08 REF XP_004396532 "PREDICTED: zinc finger and BTB domain-containing protein 32 [Odobenus rosmarus divergens]" 100.00 488 100.00 100.00 1.44e-08 REF XP_005616754 "PREDICTED: zinc finger and BTB domain-containing protein 32 isoform X3 [Canis lupus familiaris]" 100.00 486 100.00 100.00 1.85e-08 SP Q9JKD9 "RecName: Full=Zinc finger and BTB domain-containing protein 32; AltName: Full=Repressor of GATA; AltName: Full=Testis zinc fing" 100.00 465 100.00 100.00 1.33e-08 stop_ save_ ############# # Ligands # ############# save_ZN _Saveframe_category ligand _Mol_type non-polymer _Name_common "ZN (ZINC ION)" _BMRB_code . _PDB_code ZN _Molecular_mass 65.409 _Mol_charge 2 _Mol_paramagnetic no _Mol_aromatic no _Details ; Information obtained from PDB's Chemical Component Dictionary at http://wwpdb-remediation.rutgers.edu/downloads.html Downloaded on Tue Jun 9 16:52:42 2009 ; loop_ _Atom_name _PDB_atom_name _Atom_type _Atom_chirality _Atom_charge _Atom_oxidation_number _Atom_unpaired_electrons ZN ZN ZN . 2 . ? stop_ _Mol_thiol_state 'not present' _Sequence_homology_query_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $mTZD_finger3 'house mouse' 10090 Eukaryota Metazoa Mus musculus stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $mTZD_finger3 'recombinant technology' . Escherichia coli Tuner(DE3)pLacI pETBlue-1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling 'acetic acid' 50 mM '[U-99% 2H]' 'sodium chloride' 20 mM 'natural abundance' H2O 90 % 'natural abundance' D2O 10 % 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_X-PLOR_NIH _Saveframe_category software _Name 'X-PLOR NIH' _Version 2.18 loop_ _Vendor _Address _Electronic_address 'Schwieters, Kuszewski, Tjandra and Clore' . . stop_ loop_ _Task 'structure solution' stop_ _Details . save_ save_NMRView _Saveframe_category software _Name NMRView _Version 5 loop_ _Vendor _Address _Electronic_address 'Johnson, One Moon Scientific' . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ save_TALOS _Saveframe_category software _Name TALOS _Version . loop_ _Vendor _Address _Electronic_address 'Cornilescu, Delaglio and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 800 _Details . save_ save_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_2D_1H-13C_HSQC_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HSQC' _Sample_label $sample_1 save_ save_3D_CBCA(CO)NH_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $sample_1 save_ save_3D_HNCACB_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ save_3D_HNCO_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO' _Sample_label $sample_1 save_ save_3D_HN(CO)CA_6 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(CO)CA' _Sample_label $sample_1 save_ save_3D_HBHA(CO)NH_7 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HBHA(CO)NH' _Sample_label $sample_1 save_ save_3D_HCCH-TOCSY_8 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HCCH-TOCSY' _Sample_label $sample_1 save_ save_3D_1H-15N_NOESY_9 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-15N NOESY' _Sample_label $sample_1 save_ save_3D_1H-13C_NOESY_10 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-13C NOESY' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 20 . mM pH 6.0 . pH pressure 1 . atm temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.00 na indirect . . . 0.251449530 DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 DSS N 15 'methyl protons' ppm 0.00 na indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assignments_zf3 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' '2D 1H-13C HSQC' '3D CBCA(CO)NH' '3D HNCACB' '3D HNCO' '3D HN(CO)CA' '3D HBHA(CO)NH' '3D HCCH-TOCSY' '3D 1H-15N NOESY' '3D 1H-13C NOESY' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name mTZD_finger3 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 56 1 ALA H H 8.062 . 1 2 56 1 ALA HA H 4.277 . 1 3 56 1 ALA HB H 1.307 . 1 4 56 1 ALA C C 177.180 . 1 5 56 1 ALA CA C 51.985 . 1 6 56 1 ALA CB C 19.284 . 1 7 56 1 ALA N N 123.086 . 1 8 57 2 ALA H H 8.178 . 1 9 57 2 ALA HA H 4.482 . 1 10 57 2 ALA HB H 1.209 . 1 11 57 2 ALA C C 175.420 . 1 12 57 2 ALA CA C 49.982 . 1 13 57 2 ALA CB C 18.693 . 1 14 57 2 ALA N N 123.538 . 1 15 58 3 PRO HA H 4.183 . 1 16 58 3 PRO HB2 H 1.495 . 2 17 58 3 PRO HB3 H 2.022 . 2 18 58 3 PRO HD2 H 3.602 . 2 19 58 3 PRO HD3 H 3.715 . 2 20 58 3 PRO HG2 H 1.921 . 2 21 58 3 PRO HG3 H 1.921 . 2 22 58 3 PRO C C 175.790 . 1 23 58 3 PRO CA C 63.543 . 1 24 58 3 PRO CB C 31.500 . 1 25 58 3 PRO CD C 50.369 . 1 26 58 3 PRO CG C 26.912 . 1 27 59 4 TYR H H 7.498 . 1 28 59 4 TYR HA H 4.483 . 1 29 59 4 TYR HB2 H 2.444 . 2 30 59 4 TYR HB3 H 2.933 . 2 31 59 4 TYR HD1 H 6.889 . 3 32 59 4 TYR HD2 H 6.914 . 3 33 59 4 TYR HE1 H 6.770 . 3 34 59 4 TYR C C 174.240 . 1 35 59 4 TYR CA C 56.965 . 1 36 59 4 TYR CB C 39.957 . 1 37 59 4 TYR CD1 C 57.927 . 3 38 59 4 TYR CD2 C 57.927 . 3 39 59 4 TYR CE1 C 57.593 . 3 40 59 4 TYR N N 116.473 . 1 41 60 5 ARG H H 8.421 . 1 42 60 5 ARG HA H 4.956 . 1 43 60 5 ARG HB2 H 1.380 . 2 44 60 5 ARG HB3 H 1.571 . 2 45 60 5 ARG HD2 H 3.037 . 2 46 60 5 ARG HD3 H 3.037 . 2 47 60 5 ARG HG2 H 1.324 . 2 48 60 5 ARG HG3 H 1.324 . 2 49 60 5 ARG C C 175.140 . 1 50 60 5 ARG CA C 53.677 . 1 51 60 5 ARG CB C 32.558 . 1 52 60 5 ARG CD C 42.911 . 1 53 60 5 ARG CG C 26.719 . 1 54 60 5 ARG N N 121.716 . 1 55 61 6 CYS H H 8.828 . 1 56 61 6 CYS HA H 4.961 . 1 57 61 6 CYS HB2 H 2.621 . 2 58 61 6 CYS HB3 H 3.187 . 2 59 61 6 CYS C C 176.320 . 1 60 61 6 CYS CA C 57.061 . 1 61 61 6 CYS CB C 30.815 . 1 62 61 6 CYS N N 128.049 . 1 63 62 7 PRO HA H 4.474 . 1 64 62 7 PRO HB2 H 2.013 . 2 65 62 7 PRO HB3 H 2.355 . 2 66 62 7 PRO HG2 H 2.016 . 2 67 62 7 PRO HG3 H 2.199 . 2 68 62 7 PRO C C 176.640 . 1 69 62 7 PRO CA C 64.237 . 1 70 62 7 PRO CB C 32.250 . 1 71 62 7 PRO CD C 51.491 . 1 72 62 7 PRO CG C 26.924 . 1 73 63 8 LEU H H 9.014 . 1 74 63 8 LEU HA H 4.374 . 1 75 63 8 LEU HB2 H 1.089 . 2 76 63 8 LEU HB3 H 1.089 . 2 77 63 8 LEU HD1 H 0.650 . 2 78 63 8 LEU HD2 H 0.583 . 2 79 63 8 LEU HG H 0.906 . 1 80 63 8 LEU C C 177.850 . 1 81 63 8 LEU CA C 56.753 . 1 82 63 8 LEU CB C 42.297 . 1 83 63 8 LEU CD1 C 24.648 . 1 84 63 8 LEU CD2 C 22.351 . 1 85 63 8 LEU CG C 27.257 . 1 86 63 8 LEU N N 121.436 . 1 87 64 9 CYS H H 8.538 . 1 88 64 9 CYS HA H 5.097 . 1 89 64 9 CYS HB2 H 2.820 . 2 90 64 9 CYS HB3 H 3.429 . 2 91 64 9 CYS C C 175.540 . 1 92 64 9 CYS CA C 58.804 . 1 93 64 9 CYS CB C 31.840 . 1 94 64 9 CYS N N 118.190 . 1 95 65 10 ARG H H 7.945 . 1 96 65 10 ARG HA H 4.062 . 1 97 65 10 ARG HB2 H 2.053 . 2 98 65 10 ARG HB3 H 2.214 . 2 99 65 10 ARG HD2 H 3.146 . 2 100 65 10 ARG HD3 H 3.146 . 2 101 65 10 ARG C C 174.670 . 1 102 65 10 ARG CA C 58.701 . 1 103 65 10 ARG CB C 26.919 . 1 104 65 10 ARG CD C 42.514 . 1 105 65 10 ARG N N 116.493 . 1 106 66 11 ALA H H 8.334 . 1 107 66 11 ALA HA H 4.133 . 1 108 66 11 ALA HB H 1.029 . 1 109 66 11 ALA C C 177.360 . 1 110 66 11 ALA CA C 53.667 . 1 111 66 11 ALA CB C 19.435 . 1 112 66 11 ALA N N 124.818 . 1 113 67 12 GLY H H 8.551 . 1 114 67 12 GLY HA2 H 3.470 . 2 115 67 12 GLY HA3 H 4.504 . 2 116 67 12 GLY C C 174.050 . 1 117 67 12 GLY CA C 45.065 . 1 118 67 12 GLY N N 108.864 . 1 119 68 13 CYS H H 8.693 . 1 120 68 13 CYS HA H 4.989 . 1 121 68 13 CYS HB2 H 2.628 . 2 122 68 13 CYS HB3 H 2.980 . 2 123 68 13 CYS C C 181.510 . 1 124 68 13 CYS CA C 55.728 . 1 125 68 13 CYS CB C 31.020 . 1 126 68 13 CYS N N 121.792 . 1 127 69 14 PRO HA H 4.585 . 1 128 69 14 PRO HB2 H 2.134 . 2 129 69 14 PRO HB3 H 2.304 . 2 130 69 14 PRO HD2 H 3.550 . 2 131 69 14 PRO HD3 H 3.795 . 2 132 69 14 PRO HG2 H 2.014 . 2 133 69 14 PRO HG3 H 2.176 . 2 134 69 14 PRO C C 175.500 . 1 135 69 14 PRO CA C 64.105 . 1 136 69 14 PRO CB C 32.558 . 1 137 69 14 PRO CD C 50.723 . 1 138 69 14 PRO CG C 26.689 . 1 139 70 15 SER H H 7.189 . 1 140 70 15 SER HA H 4.665 . 1 141 70 15 SER HB2 H 3.942 . 2 142 70 15 SER HB3 H 4.133 . 2 143 70 15 SER C C 172.940 . 1 144 70 15 SER CA C 55.933 . 1 145 70 15 SER CB C 66.904 . 1 146 70 15 SER N N 110.436 . 1 147 71 16 LEU H H 8.656 . 1 148 71 16 LEU HA H 3.329 . 1 149 71 16 LEU HB2 H 1.250 . 2 150 71 16 LEU HB3 H 1.511 . 2 151 71 16 LEU HD1 H 0.800 . 2 152 71 16 LEU HD2 H 0.767 . 2 153 71 16 LEU HG H 1.272 . 1 154 71 16 LEU C C 178.670 . 1 155 71 16 LEU CA C 57.576 . 1 156 71 16 LEU CB C 40.965 . 1 157 71 16 LEU CD1 C 24.801 . 1 158 71 16 LEU CD2 C 22.983 . 1 159 71 16 LEU CG C 26.534 . 1 160 71 16 LEU N N 124.573 . 1 161 72 17 ALA H H 8.563 . 1 162 72 17 ALA HA H 4.092 . 1 163 72 17 ALA HB H 1.350 . 1 164 72 17 ALA C C 181.570 . 1 165 72 17 ALA CA C 55.318 . 1 166 72 17 ALA CB C 17.897 . 1 167 72 17 ALA N N 121.083 . 1 168 73 18 SER H H 7.980 . 1 169 73 18 SER HA H 4.243 . 1 170 73 18 SER HB2 H 3.942 . 2 171 73 18 SER HB3 H 3.942 . 2 172 73 18 SER C C 177.640 . 1 173 73 18 SER CA C 61.264 . 1 174 73 18 SER CB C 62.802 . 1 175 73 18 SER N N 113.924 . 1 176 74 19 MET H H 8.116 . 1 177 74 19 MET HA H 4.648 . 1 178 74 19 MET HB2 H 1.796 . 2 179 74 19 MET HB3 H 2.349 . 2 180 74 19 MET HG2 H 2.619 . 2 181 74 19 MET HG3 H 2.619 . 2 182 74 19 MET C C 178.090 . 1 183 74 19 MET CA C 59.009 . 1 184 74 19 MET CB C 32.865 . 1 185 74 19 MET CG C 31.707 . 1 186 74 19 MET N N 125.408 . 1 187 75 20 GLN H H 8.669 . 1 188 75 20 GLN HA H 3.974 . 1 189 75 20 GLN HB2 H 1.971 . 2 190 75 20 GLN HB3 H 2.217 . 2 191 75 20 GLN HE21 H 6.821 . 2 192 75 20 GLN HE22 H 7.352 . 2 193 75 20 GLN HG2 H 2.345 . 2 194 75 20 GLN HG3 H 2.514 . 2 195 75 20 GLN C C 179.160 . 1 196 75 20 GLN CA C 59.316 . 1 197 75 20 GLN CB C 27.944 . 1 198 75 20 GLN CG C 33.956 . 1 199 75 20 GLN N N 119.420 . 1 200 75 20 GLN NE2 N 110.781 . 1 201 76 21 ALA H H 7.799 . 1 202 76 21 ALA HA H 4.055 . 1 203 76 21 ALA HB H 1.491 . 1 204 76 21 ALA C C 180.420 . 1 205 76 21 ALA CA C 55.318 . 1 206 76 21 ALA CB C 17.897 . 1 207 76 21 ALA N N 121.521 . 1 208 77 22 HIS H H 8.429 . 1 209 77 22 HIS HA H 4.107 . 1 210 77 22 HIS HB2 H 3.301 . 2 211 77 22 HIS HB3 H 3.721 . 2 212 77 22 HIS HD2 H 7.308 . 3 213 77 22 HIS HE1 H 7.790 . 3 214 77 22 HIS C C 178.360 . 1 215 77 22 HIS CA C 59.726 . 1 216 77 22 HIS CB C 27.944 . 1 217 77 22 HIS N N 119.702 . 1 218 78 23 MET H H 8.678 . 1 219 78 23 MET HA H 4.219 . 1 220 78 23 MET HB2 H 2.288 . 2 221 78 23 MET HB3 H 2.288 . 2 222 78 23 MET HG2 H 3.011 . 2 223 78 23 MET HG3 H 3.011 . 2 224 78 23 MET C C 179.550 . 1 225 78 23 MET CA C 57.881 . 1 226 78 23 MET CB C 30.917 . 1 227 78 23 MET CG C 31.701 . 1 228 78 23 MET N N 117.580 . 1 229 79 24 ARG H H 7.542 . 1 230 79 24 ARG HA H 4.127 . 1 231 79 24 ARG HB2 H 1.910 . 2 232 79 24 ARG HB3 H 1.910 . 2 233 79 24 ARG HD2 H 3.283 . 2 234 79 24 ARG HD3 H 3.283 . 2 235 79 24 ARG HG2 H 1.670 . 2 236 79 24 ARG HG3 H 1.670 . 2 237 79 24 ARG C C 177.950 . 1 238 79 24 ARG CA C 58.599 . 1 239 79 24 ARG CB C 29.687 . 1 240 79 24 ARG CD C 43.212 . 1 241 79 24 ARG CG C 27.665 . 1 242 79 24 ARG N N 119.500 . 1 243 80 25 GLY H H 7.647 . 1 244 80 25 GLY HA2 H 3.667 . 2 245 80 25 GLY HA3 H 4.004 . 2 246 80 25 GLY C C 174.650 . 1 247 80 25 GLY CA C 45.476 . 1 248 80 25 GLY N N 105.594 . 1 249 81 26 HIS H H 7.325 . 1 250 81 26 HIS HA H 4.679 . 1 251 81 26 HIS HB2 H 3.167 . 2 252 81 26 HIS HB3 H 3.167 . 2 253 81 26 HIS HD2 H 6.690 . 3 254 81 26 HIS HE1 H 7.999 . 3 255 81 26 HIS C C 174.370 . 1 256 81 26 HIS CA C 55.933 . 1 257 81 26 HIS CB C 28.969 . 1 258 81 26 HIS CD2 C 66.896 . 1 259 81 26 HIS N N 118.297 . 1 260 82 27 SER H H 7.799 . 1 261 82 27 SER HA H 4.292 . 1 262 82 27 SER HB2 H 3.199 . 2 263 82 27 SER HB3 H 3.898 . 2 264 82 27 SER C C 178.620 . 1 265 82 27 SER CA C 60.547 . 1 266 82 27 SER CB C 64.995 . 1 267 82 27 SER N N 122.247 . 1 stop_ save_