data_17179 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; NMR Studies of the Backbone Flexibility and Structure of Human Growth Hormone: A Comparison of High and Low pH Conformations ; _BMRB_accession_number 17179 _BMRB_flat_file_name bmr17179.str _Entry_type original _Submission_date 2010-09-10 _Accession_date 2010-09-10 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Kasimova Marina R. . 2 Kristensen Soren M. . 3 Howe Peter W.A. . 4 Christensen Thorkild . . 5 Matthiesen Finn . . 6 Petersen Jorgen . . 7 Sorensen Hans H. . 8 Led Jens J. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count chemical_rates 2 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2010-11-10 original author . stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'NMR Studies of the Backbone Flexibility and Structure of Human Growh Hormone: A Comparison of High and Low pH Conformations' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 12054815 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Kasimova Marina R. . 2 Kristensen Soren M. . 3 Howe Peter W.A. . 4 Christensen Thorkild . . 5 Matthiesen Finn . . 6 Petersen Jorgen . . 7 Sorensen Hans H. . 8 Led Jens J. . stop_ _Journal_abbreviation 'J. Mol. Biol.' _Journal_name_full 'Journal of Molecular Biology' _Journal_volume 318 _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 679 _Page_last 695 _Year 2002 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'rhGH hydrogen exchange' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label rhGH $rhGH stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_rhGH _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common rhGH _Molecular_mass . _Mol_thiol_state 'not reported' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 191 _Mol_residue_sequence ; FPTIPLSRLFDNAMLRAHRL HQLAFDTYEEFEEAYIPKEQ KYSFLQNPQASLCFSESIPT PSNREQAQQKSNLQLLRISL LLIQSWLEPVGFLRSVFANS LVYGASDSDVYDLLKDLEEG IQTLMGRLEDGSPRTGQIFK QTYSKFDANSHNDDALLKNY GLLYCFRKDMDKVETFLRIV QCRSVEGSCGF ; loop_ _Residue_seq_code _Residue_label 1 PHE 2 PRO 3 THR 4 ILE 5 PRO 6 LEU 7 SER 8 ARG 9 LEU 10 PHE 11 ASP 12 ASN 13 ALA 14 MET 15 LEU 16 ARG 17 ALA 18 HIS 19 ARG 20 LEU 21 HIS 22 GLN 23 LEU 24 ALA 25 PHE 26 ASP 27 THR 28 TYR 29 GLU 30 GLU 31 PHE 32 GLU 33 GLU 34 ALA 35 TYR 36 ILE 37 PRO 38 LYS 39 GLU 40 GLN 41 LYS 42 TYR 43 SER 44 PHE 45 LEU 46 GLN 47 ASN 48 PRO 49 GLN 50 ALA 51 SER 52 LEU 53 CYS 54 PHE 55 SER 56 GLU 57 SER 58 ILE 59 PRO 60 THR 61 PRO 62 SER 63 ASN 64 ARG 65 GLU 66 GLN 67 ALA 68 GLN 69 GLN 70 LYS 71 SER 72 ASN 73 LEU 74 GLN 75 LEU 76 LEU 77 ARG 78 ILE 79 SER 80 LEU 81 LEU 82 LEU 83 ILE 84 GLN 85 SER 86 TRP 87 LEU 88 GLU 89 PRO 90 VAL 91 GLY 92 PHE 93 LEU 94 ARG 95 SER 96 VAL 97 PHE 98 ALA 99 ASN 100 SER 101 LEU 102 VAL 103 TYR 104 GLY 105 ALA 106 SER 107 ASP 108 SER 109 ASP 110 VAL 111 TYR 112 ASP 113 LEU 114 LEU 115 LYS 116 ASP 117 LEU 118 GLU 119 GLU 120 GLY 121 ILE 122 GLN 123 THR 124 LEU 125 MET 126 GLY 127 ARG 128 LEU 129 GLU 130 ASP 131 GLY 132 SER 133 PRO 134 ARG 135 THR 136 GLY 137 GLN 138 ILE 139 PHE 140 LYS 141 GLN 142 THR 143 TYR 144 SER 145 LYS 146 PHE 147 ASP 148 ALA 149 ASN 150 SER 151 HIS 152 ASN 153 ASP 154 ASP 155 ALA 156 LEU 157 LEU 158 LYS 159 ASN 160 TYR 161 GLY 162 LEU 163 LEU 164 TYR 165 CYS 166 PHE 167 ARG 168 LYS 169 ASP 170 MET 171 ASP 172 LYS 173 VAL 174 GLU 175 THR 176 PHE 177 LEU 178 ARG 179 ILE 180 VAL 181 GLN 182 CYS 183 ARG 184 SER 185 VAL 186 GLU 187 GLY 188 SER 189 CYS 190 GLY 191 PHE stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2014-11-10 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 1HGU "Human Growth Hormone" 100.00 191 100.00 100.00 8.37e-138 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $rhGH Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name _Details $rhGH 'obtained from a vendor' . . . . . 'Novo Nordisk' stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details 'pH was adjusted using DCl' loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $rhGH . mM 0.7 1 'natural abundance' H2O 99.996 % . . 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_software _Saveframe_category software _Name software _Version . loop_ _Vendor _Address _Electronic_address na . . stop_ loop_ _Task na stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model 'Unity Inova' _Field_strength 500 _Details . save_ ############################# # NMR applied experiments # ############################# save_HN_exchange_1 _Saveframe_category NMR_applied_experiment _Experiment_name 'HN exchange' _Sample_label $sample_1 save_ save_HN_exchange_2 _Saveframe_category NMR_applied_experiment _Experiment_name 'HN exchange' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units temperature 305 . K pH 7.0 . pH pressure ambient . atm stop_ save_ save_sample_conditions_2 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units temperature 305 . K pH 2.7 . pH pressure ambient . atm stop_ save_