data_17187 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Elucidation of the poly-L-proline binding site in Acanthamoeba profilin I by NMR spectroscopy ; _BMRB_accession_number 17187 _BMRB_flat_file_name bmr17187.str _Entry_type new _Submission_date 2010-09-10 _Accession_date 2010-09-10 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Archer Sharon J. . 2 Vinson Valda K. . 3 Pollard Thomas D. . 4 Torchia Dennis A. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count binding_constants 1 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2010-11-10 original author . stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Elucidation of the poly-L-proline binding site in Acanthamoeba profilin I by NMR spectroscopy' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 8287969 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Archer Sharon J. . 2 Vinson Valda K. . 3 Pollard Thomas D. . 4 Torchia Dennis A. . stop_ _Journal_abbreviation 'FEBS Lett.' _Journal_name_full 'FEBS Letters' _Journal_volume 337 _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 145 _Page_last 151 _Year 1994 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'profilinI/pLp complex' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label profilinI $profilinI pLp $pLp stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_profilinI _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common profilinI _Molecular_mass . _Mol_thiol_state 'not reported' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 126 _Mol_residue_sequence ; MSWQTYVDTNLVGTGAVTQA AILGLDGNTWATSAGFAVTP AQGQTLASAFNNADPIRASG FDLAGVHYVTLRADDRSIYG KKGSAGVITVKTSKSILVGV YNEKIQPGTAANVVEKLADY LIGQGF ; loop_ _Residue_seq_code _Residue_label 1 MET 2 SER 3 TRP 4 GLN 5 THR 6 TYR 7 VAL 8 ASP 9 THR 10 ASN 11 LEU 12 VAL 13 GLY 14 THR 15 GLY 16 ALA 17 VAL 18 THR 19 GLN 20 ALA 21 ALA 22 ILE 23 LEU 24 GLY 25 LEU 26 ASP 27 GLY 28 ASN 29 THR 30 TRP 31 ALA 32 THR 33 SER 34 ALA 35 GLY 36 PHE 37 ALA 38 VAL 39 THR 40 PRO 41 ALA 42 GLN 43 GLY 44 GLN 45 THR 46 LEU 47 ALA 48 SER 49 ALA 50 PHE 51 ASN 52 ASN 53 ALA 54 ASP 55 PRO 56 ILE 57 ARG 58 ALA 59 SER 60 GLY 61 PHE 62 ASP 63 LEU 64 ALA 65 GLY 66 VAL 67 HIS 68 TYR 69 VAL 70 THR 71 LEU 72 ARG 73 ALA 74 ASP 75 ASP 76 ARG 77 SER 78 ILE 79 TYR 80 GLY 81 LYS 82 LYS 83 GLY 84 SER 85 ALA 86 GLY 87 VAL 88 ILE 89 THR 90 VAL 91 LYS 92 THR 93 SER 94 LYS 95 SER 96 ILE 97 LEU 98 VAL 99 GLY 100 VAL 101 TYR 102 ASN 103 GLU 104 LYS 105 ILE 106 GLN 107 PRO 108 GLY 109 THR 110 ALA 111 ALA 112 ASN 113 VAL 114 VAL 115 GLU 116 LYS 117 LEU 118 ALA 119 ASP 120 TYR 121 LEU 122 ILE 123 GLY 124 GLN 125 GLY 126 PHE stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-10-14 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 1PRQ "Acanthamoeba Castellanii Profilin Ia" 99.21 125 100.00 100.00 1.61e-82 PDB 2PRF "Three Dimensional Solution Structure Of Acanthamoeba Profilin I" 99.21 125 100.00 100.00 1.61e-82 GB AAA27710 "profilin I [Acanthamoeba castellanii]" 100.00 126 100.00 100.00 1.57e-83 GB ELR22869 "profilin, putative [Acanthamoeba castellanii str. Neff]" 100.00 126 100.00 100.00 1.57e-83 REF XP_004351646 "profilin, putative [Acanthamoeba castellanii str. Neff]" 100.00 126 100.00 100.00 1.57e-83 SP P68696 "RecName: Full=Profilin-1A; AltName: Full=Acidic profilin IA; AltName: Full=Profilin IA" 100.00 126 100.00 100.00 1.57e-83 stop_ save_ save_pLp _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common pLp _Molecular_mass . _Mol_thiol_state 'not present' _Details . _Residue_count 10 _Mol_residue_sequence PPPPPPPPPP loop_ _Residue_seq_code _Residue_label 1 PRO 2 PRO 3 PRO 4 PRO 5 PRO 6 PRO 7 PRO 8 PRO 9 PRO 10 PRO stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 1AWI . . . . . . stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $profilinI 'Acanthamoeba castellanii' 5755 Eukaryota . Acanthamoeba castellanii stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $profilinI 'purified from the natural source' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details 'profilinI was titrated with aliquots of a concentrated solution of pLp which also contained 0.1 mM profilinI so that the concentration of profilinI remained constant' loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $profilinI 0.1 mM . . '[U-13C; U-15N]' $pLp . mM 0 8 'natural abundance' H2O 93 % . . 'natural abundance' D2O 7 % . . 'natural abundance' stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type solution _Details 'profilinI was titrated with aliquots of a concentrated solution of pLp which also contained 0.1 mM profilinI so that the concentration of profilinI remained constant' loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $profilinI 1.0 mM . . '[U-13C; U-15N]' $pLp 0-8 mM 0 8 'natural abundance' H2O 93 % . . 'natural abundance' D2O 7 % . . 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_inhouse _Saveframe_category software _Name inhouse _Version . loop_ _Vendor _Address _Electronic_address 'F. Delaglio, nmrpipe , unpublished)' . . stop_ loop_ _Task processing stop_ _Details . save_ save_NMRi _Saveframe_category software _Name NMRi _Version . loop_ _Vendor _Address _Electronic_address '(New Methods Research Inc., Syracuse, NY' . . stop_ loop_ _Task processing stop_ _Details . save_ save_inhouse2 _Saveframe_category software _Name inhouse2 _Version . loop_ _Vendor _Address _Electronic_address 'Garrett, D.S., Powers, R., Gronenborn, A.M. and Clore, G.M.' . . stop_ loop_ _Task 'data analysis' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model AMX _Field_strength 500 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_2D_1H-15N_HSQC_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_2 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units temperature 303 . K pH 6.4 . pH pressure ambient . atm stop_ save_