data_17295

#######################
#  Entry information  #
#######################

save_entry_information
   _Saveframe_category      entry_information

   _Entry_title            
;
Protein and metal cluster structure of the wheat metallothionein domain g-Ec-1. The second part of the puzzle.
;
   _BMRB_accession_number   17295
   _BMRB_flat_file_name     bmr17295.str
   _Entry_type              new
   _Submission_date         2010-11-12
   _Accession_date          2010-11-12
   _Entry_origination       author
   _NMR_STAR_version        2.1.1
   _Experimental_method     NMR
   _Details                 .

   loop_
      _Author_ordinal
      _Author_family_name
      _Author_given_name
      _Author_middle_initials
      _Author_family_title

      1 Loebus        J. .  . 
      2 Peroza        E. A. . 
      3 Bluethgen     N. .  . 
      4 Fox           T. .  . 
      5 Meyer-Klaucke W. .  . 
      6 Zerbe         O. .  . 
      7 FREISINGER    E. .  . 

   stop_

   loop_
      _Saveframe_category_type
      _Saveframe_category_type_count

      assigned_chemical_shifts 1 

   stop_

   loop_
      _Data_type
      _Data_type_count

      "1H chemical shifts"  108 
      "15N chemical shifts"  21 

   stop_

   loop_
      _Revision_date
      _Revision_keyword
      _Revision_author
      _Revision_detail

      2011-05-23 update   BMRB   'update entry citation' 
      2011-03-28 original author 'original release'      

   stop_

   loop_
      _Related_BMRB_accession_number
      _Relationship

      17294 'g-Ec-1 with Cd' 

   stop_

save_


#############################
#  Citation for this entry  #
#############################

save_citations
   _Saveframe_category           entry_citation

   _Citation_full                .
   _Citation_title              'Protein and metal cluster structure of the wheat metallothionein domain -E(c)-1: the second part of the puzzle.'
   _Citation_status              published
   _Citation_type                journal
   _CAS_abstract_code            .
   _MEDLINE_UI_code              .
   _PubMed_ID                    21437709

   loop_
      _Author_ordinal
      _Author_family_name
      _Author_given_name
      _Author_middle_initials
      _Author_family_title

      1 Loebus        Jens    .  . 
      2 Peroza        Estevao A. . 
      3 Bluthgen      Nancy   .  . 
      4 Fox           Thomas  .  . 
      5 Meyer-Klaucke Wolfram .  . 
      6 Zerbe         Oliver  .  . 
      7 Freisinger    Eva     .  . 

   stop_

   _Journal_abbreviation        'J. Biol. Inorg. Chem.'
   _Journal_name_full           'Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry'
   _Journal_volume               16
   _Journal_issue                5
   _Journal_CSD                  .
   _Book_chapter_title           .
   _Book_volume                  .
   _Book_series                  .
   _Book_ISBN                    .
   _Conference_state_province    .
   _Conference_abstract_number   .
   _Page_first                   683
   _Page_last                    694
   _Year                         2011
   _Details                      .

save_


##################################
#  Molecular system description  #
##################################

save_assembly
   _Saveframe_category         molecular_system

   _Mol_system_name           'EC PROTEIN I/II'
   _Enzyme_commission_number   .

   loop_
      _Mol_system_component_name
      _Mol_label

      'EC PROTEIN I/II' $EC_PROTEIN_I_II 
      'ZINC ION_1'      $ZN              
      'ZINC ION_2'      $ZN              

   stop_

   _System_molecular_weight    .
   _System_physical_state      native
   _System_oligomer_state      ?
   _System_paramagnetic        no
   _System_thiol_state         .
   _Database_query_date        .
   _Details                    .

save_


    ########################
    #  Monomeric polymers  #
    ########################

save_EC_PROTEIN_I_II
   _Saveframe_category                          monomeric_polymer

   _Mol_type                                    polymer
   _Mol_polymer_class                           protein
   _Name_common                                 EC_PROTEIN_I/II
   _Molecular_mass                              2464.794
   _Mol_thiol_state                            'not present'
   _Details                                     .

   	##############################
   	#  Polymer residue sequence  #
   	##############################
   
      _Residue_count                               26
   _Mol_residue_sequence                       
;
GSGCDDKCGCAVPCPGGTGC
RCTSAR
;

   loop_
      _Residue_seq_code
      _Residue_label

       1 GLY   2 SER   3 GLY   4 CYS   5 ASP 
       6 ASP   7 LYS   8 CYS   9 GLY  10 CYS 
      11 ALA  12 VAL  13 PRO  14 CYS  15 PRO 
      16 GLY  17 GLY  18 THR  19 GLY  20 CYS 
      21 ARG  22 CYS  23 THR  24 SER  25 ALA 
      26 ARG 

   stop_

   _Sequence_homology_query_date                .
   _Sequence_homology_query_revised_last_date   2015-07-08

   loop_
      _Database_name
      _Database_accession_code
      _Database_entry_mol_name
      _Sequence_query_to_submitted_percentage
      _Sequence_subject_length
      _Sequence_identity
      _Sequence_positive
      _Sequence_homology_expectation_value

      BMRB    17294  EC_PROTEIN_I/II                                                                                                 100.00  26 100.00 100.00 3.22e-07 
      PDB  2L61      "Protein And Metal Cluster Structure Of The Wheat Metallothionein Domain G-ec-1. The Second Part Of The Puzzle." 100.00  26 100.00 100.00 3.22e-07 
      PDB  2L62      "Protein And Metal Cluster Structure Of The Wheat Metallothionein Domain G-ec-1. The Second Part Of The Puzzle"  100.00  26 100.00 100.00 3.22e-07 
      DBJ  BAK06800  "predicted protein [Hordeum vulgare subsp. vulgare]"                                                              92.31  77 100.00 100.00 5.71e-06 
      EMBL CAA48349  "Ec protein, Metallothionein(class II) [Triticum aestivum]"                                                       92.31  81 100.00 100.00 5.80e-06 
      EMBL CAA48350  "Ec protein, Metallothionein(class II) [Triticum aestivum]"                                                       92.31  81 100.00 100.00 5.80e-06 
      EMBL CAD88267  "metallothionein-like protein type 4 [Hordeum vulgare subsp. vulgare]"                                            92.31  77 100.00 100.00 5.71e-06 
      GB   AAP54858  "expressed protein [Oryza sativa Japonica Group]"                                                                 69.23 134 100.00 100.00 7.79e-03 
      GB   AFK12212  "metallothionein [Hordeum vulgare]"                                                                               92.31  77 100.00 100.00 5.71e-06 
      SP   P30569    "RecName: Full=EC protein I/II; AltName: Full=Zinc metallothionein class II"                                      92.31  81 100.00 100.00 5.80e-06 

   stop_

save_


    #############
    #  Ligands  #
    #############

save_ZN
   _Saveframe_category             ligand

   _Mol_type                       non-polymer
   _Name_common                   "ZN (ZINC ION)"
   _BMRB_code                      .
   _PDB_code                       ZN
   _Molecular_mass                 65.409
   _Mol_charge                     2
   _Mol_paramagnetic               .
   _Mol_aromatic                   no
   _Details                       
;
Information obtained from PDB's Chemical Component Dictionary
at http://wwpdb-remediation.rutgers.edu/downloads.html
Downloaded on Tue Jun  9 16:52:42 2009
;

   loop_
      _Atom_name
      _PDB_atom_name
      _Atom_type
      _Atom_chirality
      _Atom_charge
      _Atom_oxidation_number
      _Atom_unpaired_electrons

      ZN ZN ZN . 2 . ? 

   stop_

   _Mol_thiol_state                .
   _Sequence_homology_query_date   .

save_


    ########################################
    #  Molecular bond linkage definitions  #
    ########################################

save_crosslink_bonds
   _Saveframe_category   crosslink_bonds


   loop_
      _Bond_order
      _Bond_type
      _Atom_one_mol_system_component_name
      _Atom_one_residue_seq_code
      _Atom_one_residue_label
      _Atom_one_atom_name
      _Atom_two_mol_system_component_name
      _Atom_two_residue_seq_code
      _Atom_two_residue_label
      _Atom_two_atom_name

      single coordination 'EC PROTEIN I/II'  4 CYS SG 'ZINC ION_1' 1 ZN ZN 
      single coordination 'EC PROTEIN I/II'  8 CYS SG 'ZINC ION_1' 1 ZN ZN 
      single coordination 'EC PROTEIN I/II' 10 CYS SG 'ZINC ION_1' 1 ZN ZN 
      single coordination 'EC PROTEIN I/II' 14 CYS SG 'ZINC ION_1' 1 ZN ZN 
      single coordination 'EC PROTEIN I/II' 20 CYS SG 'ZINC ION_1' 1 ZN ZN 
      single coordination 'EC PROTEIN I/II' 22 CYS SG 'ZINC ION_1' 1 ZN ZN 

   stop_

save_


    ####################
    #  Natural source  #
    ####################

save_natural_source
   _Saveframe_category   natural_source


   loop_
      _Mol_label
      _Organism_name_common
      _NCBI_taxonomy_ID
      _Superkingdom
      _Kingdom
      _Genus
      _Species

      $EC_PROTEIN_I_II 'Canadian hard wheat, common wheat, wheat' 4565 Eukaryota Viridiplantae Triticum aestivum 

   stop_

save_


    #########################
    #  Experimental source  #
    #########################

save_experimental_source
   _Saveframe_category   experimental_source


   loop_
      _Mol_label
      _Production_method
      _Host_organism_name_common
      _Genus
      _Species
      _Strain
      _Variant
      _Vector_name

      $EC_PROTEIN_I_II 'recombinant technology' . . . K12 BL21(DE3) PGEX-4T 
      $ZN              'obtained from a vendor' . . . .   .         .       

   stop_

save_


#####################################
#  Sample contents and methodology  #
#####################################
	 
    ########################
    #  Sample description  #
    ########################

save_sample_1
   _Saveframe_category   sample

   _Sample_type          solution
   _Details             '1.0 MM [U-99% 15N] EC PROTEIN, 15.0 MM TRIS, 50.0 MM NACL, 90% H2O/10% D2O'

   loop_
      _Mol_label
      _Concentration_value
      _Concentration_value_units
      _Isotopic_labeling

      $EC_PROTEIN_I_II  1.0 mM '[U-99% 15N]'       
       TRIS            15   mM '[U-98% 2H]'        
       D2O             10   %  '[U-99% 2H]'        
       NaCl            50   mM 'natural abundance' 
       H2O             90   %  'natural abundance' 

   stop_

save_


############################
#  Computer software used  #
############################

save_CYANA
   _Saveframe_category   software

   _Name                 CYANA
   _Version              3.0

   loop_
      _Vendor
      _Address
      _Electronic_address

      'P.GUNTERT ET AL. (CYANA), R. KORADI, P. GUNTERT, M. BILLETER (OPALP)' . . 

   stop_

   loop_
      _Task

      'structure solution' 

   stop_

   _Details              .

save_


save_OPALP
   _Saveframe_category   software

   _Name                 OPALP
   _Version              .

   loop_
      _Vendor
      _Address
      _Electronic_address

      'Luginb hl and Wuthrich' . . 

   stop_

   loop_
      _Task

      refinement 

   stop_

   _Details              .

save_


save_XEASY
   _Saveframe_category   software

   _Name                 XEASY
   _Version              1.2

   loop_
      _Vendor
      _Address
      _Electronic_address

      'Bartels C, Xia TH, Billeter M, Guentert P, Wuethrich K' . . 

   stop_

   loop_
      _Task

      'data analysis' 

   stop_

   _Details              .

save_


#########################
#  Experimental detail  #
#########################

    ##################################
    #  NMR Spectrometer definitions  #
    ##################################

save_spectrometer_1
   _Saveframe_category   NMR_spectrometer

   _Manufacturer         BRUKER
   _Model                AVANCE
   _Field_strength       700
   _Details              .

save_


save_spectrometer_2
   _Saveframe_category   NMR_spectrometer

   _Manufacturer         Bruker
   _Model                Avance
   _Field_strength       600
   _Details              .

save_


save_spectrometer_3
   _Saveframe_category   NMR_spectrometer

   _Manufacturer         Bruker
   _Model                DRX
   _Field_strength       500
   _Details              .

save_


    #############################
    #  NMR applied experiments  #
    #############################

save_2D_1H-1H_NOESY_1
   _Saveframe_category   NMR_applied_experiment

   _Experiment_name     '2D 1H-1H NOESY'
   _Sample_label        $sample_1

save_


save_2D_1H-1H_TOCSY_2
   _Saveframe_category   NMR_applied_experiment

   _Experiment_name     '2D 1H-1H TOCSY'
   _Sample_label        $sample_1

save_


save_3D_1H-15N_TOCSY_3
   _Saveframe_category   NMR_applied_experiment

   _Experiment_name     '3D 1H-15N TOCSY'
   _Sample_label        $sample_1

save_


save_3D_1H-_15N_NOESY_4
   _Saveframe_category   NMR_applied_experiment

   _Experiment_name     '3D 1H- 15N NOESY'
   _Sample_label        $sample_1

save_


#######################
#  Sample conditions  #
#######################

save_sample_conditions_1
   _Saveframe_category   sample_conditions

   _Details              .

   loop_
      _Variable_type
      _Variable_value
      _Variable_value_error
      _Variable_value_units

      'ionic strength'  50   . mM  
       pH                6.9 . pH  
       pressure          1   . atm 
       temperature     298   . K   

   stop_

save_


####################
#  NMR parameters  #
####################

    ##############################
    #  Assigned chemical shifts  #
    ##############################

	################################
	#  Chemical shift referencing  #
	################################

save_chemical_shift_reference_1
   _Saveframe_category   chemical_shift_reference

   _Details              .

   loop_
      _Mol_common_name
      _Atom_type
      _Atom_isotope_number
      _Atom_group
      _Chem_shift_units
      _Chem_shift_value
      _Reference_method
      _Reference_type
      _External_reference_sample_geometry
      _External_reference_location
      _External_reference_axis
      _Indirect_shift_ratio

      water H 1 protons ppm 4.74 internal direct . . . 1.0 

   stop_

save_


	###################################
	#  Assigned chemical shift lists  #
	###################################

###################################################################
#       Chemical Shift Ambiguity Index Value Definitions          #
#                                                                 #
# The values other than 1 are used for those atoms with different #
# chemical shifts that cannot be assigned to stereospecific atoms #
# or to specific residues or chains.                              #
#                                                                 #
#   Index Value            Definition                             #
#                                                                 #
#      1             Unique (including isolated methyl protons,   #
#                         geminal atoms, and geminal methyl       #
#                         groups with identical chemical shifts)  #
#                         (e.g. ILE HD11, HD12, HD13 protons)     #
#      2             Ambiguity of geminal atoms or geminal methyl #
#                         proton groups (e.g. ASP HB2 and HB3     #
#                         protons, LEU CD1 and CD2 carbons, or    #
#                         LEU HD11, HD12, HD13 and HD21, HD22,    #
#                         HD23 methyl protons)                    #
#      3             Aromatic atoms on opposite sides of          #
#                         symmetrical rings (e.g. TYR HE1 and HE2 #
#                         protons)                                #
#      4             Intraresidue ambiguities (e.g. LYS HG and    #
#                         HD protons or TRP HZ2 and HZ3 protons)  #
#      5             Interresidue ambiguities (LYS 12 vs. LYS 27) #
#      6             Intermolecular ambiguities (e.g. ASP 31 CA   #
#                         in monomer 1 and ASP 31 CA in monomer 2 #
#                         of an asymmetrical homodimer, duplex    #
#                         DNA assignments, or other assignments   #
#                         that may apply to atoms in one or more  #
#                         molecule in the molecular assembly)     #
#      9             Ambiguous, specific ambiguity not defined    #
#                                                                 #
###################################################################
save_assigned_chem_shift_list_1
   _Saveframe_category               assigned_chemical_shifts

   _Details                          .

   loop_
      _Software_label

      $XEASY 

   stop_

   loop_
      _Experiment_label

      '2D 1H-1H NOESY'   
      '2D 1H-1H TOCSY'   
      '3D 1H-15N TOCSY'  
      '3D 1H- 15N NOESY' 

   stop_

   loop_
      _Sample_label

      $sample_1 

   stop_

   _Sample_conditions_label         $sample_conditions_1
   _Chem_shift_reference_set_label  $chemical_shift_reference_1
   _Mol_system_component_name       'EC PROTEIN I/II'
   _Text_data_format                 .
   _Text_data                        .

   loop_
      _Atom_shift_assign_ID
      _Residue_author_seq_code
      _Residue_seq_code
      _Residue_label
      _Atom_name
      _Atom_type
      _Chem_shift_value
      _Chem_shift_value_error
      _Chem_shift_ambiguity_code

        1  3  3 GLY H   H   8.706 0.020 1 
        2  3  3 GLY HA2 H   3.716 0.020 2 
        3  3  3 GLY HA3 H   4.035 0.020 2 
        4  3  3 GLY N   N 111.481 0.400 1 
        5  4  4 CYS H   H   8.143 0.020 1 
        6  4  4 CYS HA  H   4.508 0.020 1 
        7  4  4 CYS HB2 H   2.751 0.020 2 
        8  4  4 CYS HB3 H   3.563 0.020 2 
        9  4  4 CYS N   N 120.743 0.400 1 
       10  5  5 ASP H   H   9.043 0.020 1 
       11  5  5 ASP HA  H   4.565 0.020 1 
       12  5  5 ASP HB2 H   2.673 0.020 2 
       13  5  5 ASP HB3 H   2.874 0.020 2 
       14  5  5 ASP N   N 125.018 0.400 1 
       15  6  6 ASP H   H   8.600 0.020 1 
       16  6  6 ASP HA  H   4.256 0.020 1 
       17  6  6 ASP HB2 H   2.470 0.020 2 
       18  6  6 ASP HB3 H   2.753 0.020 2 
       19  6  6 ASP N   N 116.125 0.400 1 
       20  7  7 LYS H   H   8.430 0.020 1 
       21  7  7 LYS HA  H   4.458 0.020 1 
       22  7  7 LYS HB2 H   1.663 0.020 1 
       23  7  7 LYS HB3 H   1.663 0.020 1 
       24  7  7 LYS HG2 H   1.190 0.020 1 
       25  7  7 LYS HG3 H   1.190 0.020 1 
       26  7  7 LYS HD2 H   1.430 0.020 1 
       27  7  7 LYS HD3 H   1.430 0.020 1 
       28  7  7 LYS HE2 H   2.819 0.020 1 
       29  7  7 LYS HE3 H   2.819 0.020 1 
       30  7  7 LYS N   N 117.741 0.400 1 
       31  8  8 CYS H   H   7.428 0.020 1 
       32  8  8 CYS HA  H   4.303 0.020 1 
       33  8  8 CYS HB2 H   3.005 0.020 1 
       34  8  8 CYS HB3 H   3.005 0.020 1 
       35  8  8 CYS N   N 119.025 0.400 1 
       36  9  9 GLY H   H   8.627 0.020 1 
       37  9  9 GLY HA2 H   3.412 0.020 2 
       38  9  9 GLY HA3 H   4.405 0.020 2 
       39  9  9 GLY N   N 112.263 0.400 1 
       40 10 10 CYS H   H   8.690 0.020 1 
       41 10 10 CYS HA  H   4.264 0.020 1 
       42 10 10 CYS HB2 H   3.181 0.020 2 
       43 10 10 CYS HB3 H   3.520 0.020 2 
       44 10 10 CYS N   N 123.700 0.400 1 
       45 11 11 ALA H   H   8.411 0.020 1 
       46 11 11 ALA HA  H   3.976 0.020 1 
       47 11 11 ALA HB  H   1.191 0.020 1 
       48 11 11 ALA N   N 124.393 0.400 1 
       49 12 12 VAL H   H   7.956 0.020 1 
       50 12 12 VAL HA  H   4.042 0.020 1 
       51 12 12 VAL HB  H   1.735 0.020 1 
       52 12 12 VAL HG1 H   0.640 0.020 2 
       53 12 12 VAL HG2 H   0.640 0.020 2 
       54 12 12 VAL N   N 118.562 0.400 1 
       55 13 13 PRO HA  H   4.579 0.020 1 
       56 13 13 PRO HB2 H   1.737 0.020 2 
       57 13 13 PRO HB3 H   2.157 0.020 2 
       58 13 13 PRO HG2 H   1.641 0.020 1 
       59 13 13 PRO HG3 H   1.641 0.020 1 
       60 13 13 PRO HD2 H   3.301 0.020 2 
       61 13 13 PRO HD3 H   3.402 0.020 2 
       62 14 14 CYS H   H   8.286 0.020 1 
       63 14 14 CYS HA  H   4.386 0.020 1 
       64 14 14 CYS HB2 H   2.723 0.020 2 
       65 14 14 CYS HB3 H   3.131 0.020 2 
       66 14 14 CYS N   N 124.784 0.400 1 
       67 15 15 PRO HA  H   4.344 0.020 1 
       68 15 15 PRO HB2 H   1.904 0.020 2 
       69 15 15 PRO HB3 H   2.061 0.020 2 
       70 15 15 PRO HG2 H   1.762 0.020 1 
       71 15 15 PRO HG3 H   1.762 0.020 1 
       72 15 15 PRO HD2 H   3.664 0.020 2 
       73 15 15 PRO HD3 H   4.072 0.020 2 
       74 16 16 GLY H   H   8.359 0.020 1 
       75 16 16 GLY HA2 H   3.574 0.020 2 
       76 16 16 GLY HA3 H   4.028 0.020 2 
       77 16 16 GLY N   N 110.530 0.400 1 
       78 17 17 GLY H   H   8.332 0.020 1 
       79 17 17 GLY HA2 H   3.630 0.020 2 
       80 17 17 GLY HA3 H   3.880 0.020 2 
       81 17 17 GLY N   N 107.409 0.400 1 
       82 18 18 THR HA  H   4.169 0.020 1 
       83 18 18 THR HB  H   3.933 0.020 1 
       84 18 18 THR HG2 H   1.116 0.020 1 
       85 19 19 GLY H   H   8.061 0.020 1 
       86 19 19 GLY HA2 H   3.462 0.020 2 
       87 19 19 GLY HA3 H   3.992 0.020 2 
       88 19 19 GLY N   N 108.276 0.400 1 
       89 20 20 CYS H   H   7.047 0.020 1 
       90 20 20 CYS HA  H   4.337 0.020 1 
       91 20 20 CYS HB2 H   2.124 0.020 2 
       92 20 20 CYS HB3 H   2.838 0.020 2 
       93 20 20 CYS N   N 122.822 0.400 1 
       94 21 21 ARG H   H   8.088 0.020 1 
       95 21 21 ARG HA  H   4.203 0.020 1 
       96 21 21 ARG HB2 H   1.854 0.020 1 
       97 21 21 ARG HB3 H   1.854 0.020 1 
       98 21 21 ARG HG2 H   1.613 0.020 1 
       99 21 21 ARG HG3 H   1.613 0.020 1 
      100 21 21 ARG HD2 H   3.028 0.020 1 
      101 21 21 ARG HD3 H   3.028 0.020 1 
      102 21 21 ARG N   N 131.038 0.400 1 
      103 22 22 CYS H   H   8.379 0.020 1 
      104 22 22 CYS HA  H   4.207 0.020 1 
      105 22 22 CYS HB2 H   2.441 0.020 2 
      106 22 22 CYS HB3 H   2.840 0.020 2 
      107 22 22 CYS N   N 122.500 0.400 1 
      108 23 23 THR H   H   7.530 0.020 1 
      109 23 23 THR HB  H   4.124 0.020 1 
      110 23 23 THR HG2 H   1.044 0.020 1 
      111 23 23 THR N   N 113.838 0.400 1 
      112 24 24 SER H   H   7.925 0.020 1 
      113 24 24 SER HA  H   4.277 0.020 1 
      114 24 24 SER HB2 H   3.732 0.020 1 
      115 24 24 SER HB3 H   3.732 0.020 1 
      116 24 24 SER N   N 117.756 0.400 1 
      117 25 25 ALA H   H   8.006 0.020 1 
      118 25 25 ALA HA  H   4.176 0.020 1 
      119 25 25 ALA HB  H   1.225 0.020 1 
      120 25 25 ALA N   N 126.257 0.400 1 
      121 26 26 ARG H   H   7.713 0.020 1 
      122 26 26 ARG HA  H   3.989 0.020 1 
      123 26 26 ARG HB2 H   1.533 0.020 2 
      124 26 26 ARG HB3 H   1.688 0.020 2 
      125 26 26 ARG HG2 H   1.430 0.020 1 
      126 26 26 ARG HG3 H   1.430 0.020 1 
      127 26 26 ARG HD2 H   3.027 0.020 1 
      128 26 26 ARG HD3 H   3.027 0.020 1 
      129 26 26 ARG N   N 125.425 0.400 1 

   stop_

save_