data_17383 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Crimean Congo Hemorrhagic Fever Gn zinc finger ; _BMRB_accession_number 17383 _BMRB_flat_file_name bmr17383.str _Entry_type original _Submission_date 2010-12-27 _Accession_date 2010-12-27 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details 'solution nmr structure of the crimean congo hemorrhagic fever cytoplasmic tail' loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Estrada David F. . 2 'De Guzman' Roberto N. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 heteronucl_NOE 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 155 "13C chemical shifts" 148 "15N chemical shifts" 70 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2011-06-24 update BMRB 'update entry citation' 2011-05-03 original author 'original release' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Structural characterization of the crimean-congo hemorrhagic Fever virus gn tail provides insight into virus assembly.' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 21507948 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Estrada 'D. Fernando' . . 2 'De Guzman' Roberto N. . stop_ _Journal_abbreviation 'J. Biol. Chem.' _Journal_name_full 'The Journal of biological chemistry' _Journal_volume 286 _Journal_issue 24 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 21678 _Page_last 21686 _Year 2011 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'Crimean Congo Hemorrhagic Fever Virus Cytoplasmic Gn Tail' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'Crimean Congo Hemorrhagic Fever Virus Cytoplasmic Gn Tail' $CRIMEAN_CONGO_GN_ZINC_FINGER 'ZINC ION 1' $ZN 'ZINC ION 2' $ZN stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_CRIMEAN_CONGO_GN_ZINC_FINGER _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common CRIMEAN_CONGO_GN_ZINC_FINGER _Molecular_mass 8936.340 _Mol_thiol_state 'all other bound' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 77 _Mol_residue_sequence ; RELKPQTCTICETTPVNAID AEMHDLNCSYNICPYCASRL TSDGLARHVTQCPKRKEKVE ETELYLNLERIPWVVRK ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 29 ARG 2 30 GLU 3 31 LEU 4 32 LYS 5 33 PRO 6 34 GLN 7 35 THR 8 36 CYS 9 37 THR 10 38 ILE 11 39 CYS 12 40 GLU 13 41 THR 14 42 THR 15 43 PRO 16 44 VAL 17 45 ASN 18 46 ALA 19 47 ILE 20 48 ASP 21 49 ALA 22 50 GLU 23 51 MET 24 52 HIS 25 53 ASP 26 54 LEU 27 55 ASN 28 56 CYS 29 57 SER 30 58 TYR 31 59 ASN 32 60 ILE 33 61 CYS 34 62 PRO 35 63 TYR 36 64 CYS 37 65 ALA 38 66 SER 39 67 ARG 40 68 LEU 41 69 THR 42 70 SER 43 71 ASP 44 72 GLY 45 73 LEU 46 74 ALA 47 75 ARG 48 76 HIS 49 77 VAL 50 78 THR 51 79 GLN 52 80 CYS 53 81 PRO 54 82 LYS 55 83 ARG 56 84 LYS 57 85 GLU 58 86 LYS 59 87 VAL 60 88 GLU 61 89 GLU 62 90 THR 63 91 GLU 64 92 LEU 65 93 TYR 66 94 LEU 67 95 ASN 68 96 LEU 69 97 GLU 70 98 ARG 71 99 ILE 72 100 PRO 73 101 TRP 74 102 VAL 75 103 VAL 76 104 ARG 77 105 LYS stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-02-05 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 2L7X "Crimean Congo Hemorrhagic Fever Gn Zinc Finger" 100.00 77 100.00 100.00 1.23e-48 DBJ BAB84574 "envelope glycoprotein precursor [Crimean-Congo hemorrhagic fever virus]" 100.00 1689 98.70 98.70 6.86e-44 DBJ BAB84575 "envelope glycoprotein precursor [Crimean-Congo hemorrhagic fever virus]" 100.00 1689 98.70 98.70 6.41e-44 DBJ BAB84577 "envelope glycoprotein precursor [Crimean-Congo hemorrhagic fever virus]" 100.00 1689 97.40 98.70 1.02e-43 DBJ BAB84578 "envelope glycoprotein precursor [Crimean-Congo hemorrhagic fever virus]" 100.00 1689 97.40 98.70 1.01e-43 EMBL CAD61343 "envelope glycoprotein precursor [Crimean-Congo hemorrhagic fever virus]" 100.00 1689 100.00 100.00 1.57e-44 EMBL CAD61345 "envelope glycoprotein precursor [Crimean-Congo hemorrhagic fever virus]" 100.00 1685 98.70 100.00 3.83e-44 GB AAA86616 "envelope glycoprotein precursor [Crimean-Congo hemorrhagic fever virus]" 100.00 1684 98.70 98.70 1.18e-43 GB AAK18286 "envelope glycoprotein precursor [Crimean-Congo hemorrhagic fever virus strain BA88166]" 100.00 1689 97.40 98.70 1.05e-43 GB AAM48106 "glycoprotein precursor [Crimean-Congo hemorrhagic fever virus]" 100.00 1684 98.70 98.70 1.19e-43 GB AAM48107 "glycoprotein precursor [Crimean-Congo hemorrhagic fever virus]" 100.00 1689 97.40 98.70 1.15e-43 GB AAP29978 "polyprotein [Crimean-Congo hemorrhagic fever virus]" 100.00 1689 97.40 98.70 9.63e-44 REF NP_950235 "glycoprotein precursor [Crimean-Congo hemorrhagic fever virus]" 100.00 1684 98.70 98.70 1.19e-43 SP Q8JSZ3 "RecName: Full=Envelope glycoprotein; Short=GP; AltName: Full=M polyprotein; Contains: RecName: Full=Mucin-like variable region;" 100.00 1684 98.70 98.70 1.19e-43 stop_ save_ ############# # Ligands # ############# save_ZN _Saveframe_category ligand _Mol_type non-polymer _Name_common "ZN (ZINC ION)" _BMRB_code . _PDB_code ZN _Molecular_mass 65.409 _Mol_charge 2 _Mol_paramagnetic . _Mol_aromatic no _Details ; Information obtained from PDB's Chemical Component Dictionary at http://wwpdb-remediation.rutgers.edu/downloads.html Downloaded on Fri Dec 9 11:09:24 2011 ; loop_ _Atom_name _PDB_atom_name _Atom_type _Atom_chirality _Atom_charge _Atom_oxidation_number _Atom_unpaired_electrons ZN ZN ZN . 2 . ? stop_ _Mol_thiol_state . _Sequence_homology_query_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _Organism_acronym _ICTVdb_decimal_code _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species _Strain $CRIMEAN_CONGO_GN_ZINC_FINGER 'Crimean-Congo hemorrhagic fever virus' CCHFV 00.011.0.03.002. 11593 viruses . nairovirus 'Crimean-Congo hemorrhagic fever virus' SPU103/87 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name _Details $CRIMEAN_CONGO_GN_ZINC_FINGER 'recombinant technology' . Escherichia coli BL21 pET21a 'modified to include GB1 solubility enhancement tag at N terminus' stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_cchfv_gn_729-805_n15 _Saveframe_category sample _Sample_type solution _Details '500 ul' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $CRIMEAN_CONGO_GN_ZINC_FINGER .7 mM '[U-99% 15N]' 'sodium phosphate' 20 mM 'natural abundance' 'sodium chloride' 20 mM 'natural abundance' 'zinc sulfate' 0.1 mM 'natural abundance' H2O 90 % 'natural abundance' D2O 10 % 'natural abundance' stop_ save_ save_cchfv_gn_729-805_n15_c13 _Saveframe_category sample _Sample_type solution _Details '500 ul' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $CRIMEAN_CONGO_GN_ZINC_FINGER .5 mM '[U-99% 13C; U-99% 15N]' 'sodium phosphate' 20 mM 'natural abundance' 'sodium chloride' 20 mM 'natural abundance' 'zinc sulfate' 0.1 mM 'natural abundance' H2O 90 % 'natural abundance' D2O 10 % 'natural abundance' stop_ save_ save_cchfv_gn_729-805_n15_c13_D2O _Saveframe_category sample _Sample_type solution _Details '500 ul' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $CRIMEAN_CONGO_GN_ZINC_FINGER .7 mM '[U-99% 13C; U-99% 15N]' 'sodium phosphate' 20 mM 'natural abundance' 'sodium chloride' 20 mM 'natural abundance' 'zinc sulfate' 0.1 mM 'natural abundance' D2O 100 % 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_CYANA _Saveframe_category software _Name CYANA _Version . loop_ _Vendor _Address _Electronic_address 'G ntert P.' . . stop_ loop_ _Task refinement stop_ _Details . save_ save_AMBER _Saveframe_category software _Name AMBER _Version 7 loop_ _Vendor _Address _Electronic_address 'Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm' . . stop_ loop_ _Task refinement stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 800 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $cchfv_gn_729-805_n15 save_ save_3D_1H-15N_NOESY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-15N NOESY' _Sample_label $cchfv_gn_729-805_n15 save_ save_3D_HNCA_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCA' _Sample_label $cchfv_gn_729-805_n15_c13 save_ save_3D_HNCACB_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $cchfv_gn_729-805_n15_c13 save_ save_3D_HBHA(CO)NH_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HBHA(CO)NH' _Sample_label $cchfv_gn_729-805_n15_c13 save_ save_3D_CBCA(CO)NH_6 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $cchfv_gn_729-805_n15_c13 save_ save_3D_1H-13C_NOESY_7 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-13C NOESY' _Sample_label $cchfv_gn_729-805_n15_c13_D2O save_ save_2D_1H-13C_HSQC_aliphatic_8 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HSQC aliphatic' _Sample_label $cchfv_gn_729-805_n15_c13_D2O save_ save_2D_1H-15N_HeteroNuclear_NOE_9 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HeteroNuclear NOE' _Sample_label $cchfv_gn_729-805_n15 save_ save_NMR_spectrometer_expt _Saveframe_category NMR_applied_experiment _Experiment_name . _BMRB_pulse_sequence_accession_number . _Details . save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 6.9 . pH pressure 1 . atm temperature 273 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio _Indirect_shift_ratio_citation_label _Correction_value_citation_label DSS C 13 'methyl protons' ppm 0.00 . indirect . . . 0.251449530 $entry_citation $entry_citation DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 $entry_citation $entry_citation DSS N 15 'methyl protons' ppm 0.00 . indirect . . . 0.101329118 $entry_citation $entry_citation stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '3D 1H-15N NOESY' '3D HNCA' '3D HNCACB' '3D CBCA(CO)NH' stop_ loop_ _Sample_label $cchfv_gn_729-805_n15 $cchfv_gn_729-805_n15_c13 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'Crimean Congo Hemorrhagic Fever Virus Cytoplasmic Gn Tail' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 29 1 ARG H H 8.539 . 1 2 29 1 ARG HA H 4.251 . 1 3 29 1 ARG CA C 56.423 . 1 4 29 1 ARG CB C 30.601 . 1 5 29 1 ARG N N 122.619 . 1 6 30 2 GLU H H 8.404 . 1 7 30 2 GLU HA H 4.251 . 1 8 30 2 GLU CA C 56.474 . 1 9 30 2 GLU CB C 30.388 . 1 10 30 2 GLU N N 121.916 . 1 11 31 3 LEU H H 8.177 . 1 12 31 3 LEU HA H 4.289 . 1 13 31 3 LEU CA C 55.085 . 1 14 31 3 LEU CB C 42.411 . 1 15 31 3 LEU N N 123.439 . 1 16 32 4 LYS H H 8.228 . 1 17 32 4 LYS HA H 4.520 . 1 18 32 4 LYS CA C 53.895 . 1 19 32 4 LYS CB C 32.404 . 1 20 32 4 LYS N N 123.645 . 1 21 33 5 PRO HA H 4.289 . 1 22 33 5 PRO CA C 62.794 . 1 23 33 5 PRO CB C 32.542 . 1 24 34 6 GLN H H 8.278 . 1 25 34 6 GLN HA H 4.206 . 1 26 34 6 GLN CA C 55.153 . 1 27 34 6 GLN CB C 29.843 . 1 28 34 6 GLN N N 119.956 . 1 29 35 7 THR H H 8.102 . 1 30 35 7 THR HA H 3.726 . 1 31 35 7 THR CA C 64.131 . 1 32 35 7 THR CB C 70.033 . 1 33 35 7 THR N N 116.865 . 1 34 36 8 CYS H H 8.160 . 1 35 36 8 CYS HA H 4.439 . 1 36 36 8 CYS CA C 59.591 . 1 37 36 8 CYS CB C 30.329 . 1 38 36 8 CYS N N 128.681 . 1 39 37 9 THR H H 9.553 . 1 40 37 9 THR HB H 4.064 . 1 41 37 9 THR CA C 63.839 . 1 42 37 9 THR CB C 69.111 . 1 43 37 9 THR N N 121.780 . 1 44 38 10 ILE H H 8.434 . 1 45 38 10 ILE HA H 4.101 . 1 46 38 10 ILE HB H 1.776 . 1 47 38 10 ILE CA C 63.370 . 1 48 38 10 ILE CB C 38.176 . 1 49 38 10 ILE N N 122.421 . 1 50 39 11 CYS H H 8.240 . 1 51 39 11 CYS HA H 4.589 . 1 52 39 11 CYS CA C 59.322 . 1 53 39 11 CYS CB C 31.036 . 1 54 39 11 CYS N N 117.943 . 1 55 40 12 GLU H H 7.496 . 1 56 40 12 GLU HA H 3.914 . 1 57 40 12 GLU CA C 58.070 . 1 58 40 12 GLU CB C 26.699 . 1 59 40 12 GLU N N 113.915 . 1 60 41 13 THR H H 8.463 . 1 61 41 13 THR HA H 4.251 . 1 62 41 13 THR HB H 4.626 . 1 63 41 13 THR CA C 62.208 . 1 64 41 13 THR CB C 70.690 . 1 65 41 13 THR N N 115.758 . 1 66 42 14 THR H H 8.791 . 1 67 42 14 THR HA H 4.422 . 1 68 42 14 THR CA C 60.862 . 1 69 42 14 THR N N 121.689 . 1 70 43 15 PRO HA H 4.326 . 1 71 43 15 PRO CA C 61.956 . 1 72 44 16 VAL H H 9.959 . 1 73 44 16 VAL HA H 4.026 . 1 74 44 16 VAL HB H 2.301 . 1 75 44 16 VAL CA C 63.696 . 1 76 44 16 VAL CB C 32.182 . 1 77 44 16 VAL N N 115.711 . 1 78 45 17 ASN H H 7.442 . 1 79 45 17 ASN HA H 4.514 . 1 80 45 17 ASN CA C 52.236 . 1 81 45 17 ASN CB C 39.547 . 1 82 45 17 ASN N N 112.059 . 1 83 46 18 ALA H H 8.841 . 1 84 46 18 ALA HA H 4.176 . 1 85 46 18 ALA CA C 55.854 . 1 86 46 18 ALA CB C 18.389 . 1 87 46 18 ALA N N 121.144 . 1 88 47 19 ILE H H 7.816 . 1 89 47 19 ILE HA H 3.914 . 1 90 47 19 ILE HB H 1.851 . 1 91 47 19 ILE CA C 64.077 . 1 92 47 19 ILE CB C 37.537 . 1 93 47 19 ILE N N 120.643 . 1 94 48 20 ASP H H 8.261 . 1 95 48 20 ASP HA H 4.694 . 1 96 48 20 ASP CA C 56.461 . 1 97 48 20 ASP CB C 41.389 . 1 98 48 20 ASP N N 120.423 . 1 99 49 21 ALA HA H 3.839 . 1 100 49 21 ALA CA C 56.109 . 1 101 49 21 ALA CB C 17.850 . 1 102 50 22 GLU H H 7.715 . 1 103 50 22 GLU HA H 4.214 . 1 104 50 22 GLU CA C 59.496 . 1 105 50 22 GLU CB C 29.367 . 1 106 50 22 GLU N N 119.591 . 1 107 51 23 MET H H 7.992 . 1 108 51 23 MET HA H 4.269 . 1 109 51 23 MET CA C 58.283 . 1 110 51 23 MET CB C 31.891 . 1 111 51 23 MET N N 118.274 . 1 112 52 24 HIS H H 8.068 . 1 113 52 24 HIS HA H 4.739 . 1 114 52 24 HIS CA C 60.871 . 1 115 52 24 HIS CB C 29.995 . 1 116 52 24 HIS N N 120.999 . 1 117 53 25 ASP H H 8.068 . 1 118 53 25 ASP HA H 4.476 . 1 119 53 25 ASP CA C 57.838 . 1 120 53 25 ASP CB C 40.828 . 1 121 53 25 ASP N N 120.713 . 1 122 54 26 LEU H H 7.939 . 1 123 54 26 LEU HA H 4.364 . 1 124 54 26 LEU CA C 56.467 . 1 125 54 26 LEU CB C 43.880 . 1 126 54 26 LEU N N 116.467 . 1 127 55 27 ASN H H 7.547 . 1 128 55 27 ASN HA H 5.001 . 1 129 55 27 ASN CA C 56.913 . 1 130 55 27 ASN CB C 41.143 . 1 131 55 27 ASN N N 114.750 . 1 132 56 28 CYS H H 9.048 . 1 133 56 28 CYS HA H 4.064 . 1 134 56 28 CYS CA C 66.429 . 1 135 56 28 CYS CB C 28.008 . 1 136 56 28 CYS N N 124.595 . 1 137 57 29 SER H H 8.261 . 1 138 57 29 SER HA H 3.914 . 1 139 57 29 SER CA C 60.916 . 1 140 57 29 SER CB C 62.228 . 1 141 57 29 SER N N 112.566 . 1 142 58 30 TYR H H 7.530 . 1 143 58 30 TYR HA H 4.551 . 1 144 58 30 TYR CA C 57.310 . 1 145 58 30 TYR CB C 38.507 . 1 146 58 30 TYR N N 119.570 . 1 147 59 31 ASN H H 8.169 . 1 148 59 31 ASN HA H 4.139 . 1 149 59 31 ASN CA C 55.159 . 1 150 59 31 ASN CB C 36.838 . 1 151 59 31 ASN N N 114.116 . 1 152 60 32 ILE H H 7.328 . 1 153 60 32 ILE HA H 4.502 . 1 154 60 32 ILE CA C 58.937 . 1 155 60 32 ILE CB C 40.207 . 1 156 60 32 ILE N N 119.165 . 1 157 61 33 CYS HA H 4.373 . 1 158 61 33 CYS CA C 58.960 . 1 159 61 33 CYS CB C 40.229 . 1 160 62 34 PRO HA H 3.951 . 1 161 62 34 PRO CA C 60.274 . 1 162 62 34 PRO CB C 31.579 . 1 163 63 35 TYR H H 9.245 . 1 164 63 35 TYR HA H 4.551 . 1 165 63 35 TYR CA C 59.625 . 1 166 63 35 TYR CB C 39.249 . 1 167 63 35 TYR N N 120.011 . 1 168 64 36 CYS H H 8.505 . 1 169 64 36 CYS HA H 5.001 . 1 170 64 36 CYS CA C 58.158 . 1 171 64 36 CYS CB C 32.484 . 1 172 64 36 CYS N N 117.569 . 1 173 65 37 ALA H H 7.774 . 1 174 65 37 ALA HA H 4.064 . 1 175 65 37 ALA CA C 53.975 . 1 176 65 37 ALA CB C 16.705 . 1 177 65 37 ALA N N 121.088 . 1 178 66 38 SER H H 8.131 . 1 179 66 38 SER HA H 4.214 . 1 180 66 38 SER CA C 60.741 . 1 181 66 38 SER CB C 62.814 . 1 182 66 38 SER N N 117.192 . 1 183 67 39 ARG H H 8.425 . 1 184 67 39 ARG HA H 4.514 . 1 185 67 39 ARG CA C 56.361 . 1 186 67 39 ARG CB C 30.086 . 1 187 67 39 ARG N N 123.723 . 1 188 68 40 LEU H H 8.555 . 1 189 68 40 LEU HA H 4.712 . 1 190 68 40 LEU CA C 52.718 . 1 191 68 40 LEU CB C 43.474 . 1 192 68 40 LEU N N 129.550 . 1 193 69 41 THR H H 7.544 . 1 194 69 41 THR HA H 4.551 . 1 195 69 41 THR CA C 60.455 . 1 196 69 41 THR CB C 70.951 . 1 197 69 41 THR N N 108.645 . 1 198 70 42 SER H H 8.934 . 1 199 70 42 SER HA H 4.139 . 1 200 70 42 SER CA C 61.501 . 1 201 70 42 SER CB C 62.535 . 1 202 70 42 SER N N 117.320 . 1 203 71 43 ASP H H 8.354 . 1 204 71 43 ASP HA H 4.589 . 1 205 71 43 ASP CA C 55.178 . 1 206 71 43 ASP CB C 40.374 . 1 207 71 43 ASP N N 117.899 . 1 208 72 44 GLY H H 7.715 . 1 209 72 44 GLY HA2 H 3.714 . 2 210 72 44 GLY HA3 H 4.278 . 2 211 72 44 GLY CA C 45.266 . 1 212 72 44 GLY N N 106.453 . 1 213 73 45 LEU H H 7.702 . 1 214 73 45 LEU HA H 4.026 . 1 215 73 45 LEU CA C 59.430 . 1 216 73 45 LEU CB C 41.957 . 1 217 73 45 LEU N N 123.902 . 1 218 74 46 ALA H H 8.732 . 1 219 74 46 ALA HA H 4.289 . 1 220 74 46 ALA CA C 56.229 . 1 221 74 46 ALA CB C 18.007 . 1 222 74 46 ALA N N 121.273 . 1 223 75 47 ARG H H 8.287 . 1 224 75 47 ARG HA H 4.064 . 1 225 75 47 ARG CA C 58.343 . 1 226 75 47 ARG CB C 30.122 . 1 227 75 47 ARG N N 117.070 . 1 228 76 48 HIS H H 7.799 . 1 229 76 48 HIS HA H 4.251 . 1 230 76 48 HIS CA C 59.690 . 1 231 76 48 HIS CB C 29.060 . 1 232 76 48 HIS N N 116.129 . 1 233 77 49 VAL H H 8.869 . 1 234 77 49 VAL HA H 3.576 . 1 235 77 49 VAL HB H 2.263 . 1 236 77 49 VAL CA C 67.776 . 1 237 77 49 VAL CB C 32.400 . 1 238 77 49 VAL N N 119.033 . 1 239 78 50 THR H H 7.723 . 1 240 78 50 THR HA H 3.870 . 1 241 78 50 THR CA C 65.714 . 1 242 78 50 THR CB C 69.058 . 1 243 78 50 THR N N 110.079 . 1 244 79 51 GLN H H 7.017 . 1 245 79 51 GLN HA H 4.476 . 1 246 79 51 GLN CA C 54.277 . 1 247 79 51 GLN CB C 29.278 . 1 248 79 51 GLN N N 116.322 . 1 249 80 52 CYS H H 7.253 . 1 250 80 52 CYS HA H 3.973 . 1 251 80 52 CYS CA C 58.624 . 1 252 80 52 CYS N N 127.046 . 1 253 81 53 PRO HA H 4.326 . 1 254 81 53 PRO CA C 65.023 . 1 255 82 54 LYS H H 8.163 . 1 256 82 54 LYS HA H 4.289 . 1 257 82 54 LYS CA C 56.558 . 1 258 82 54 LYS CB C 32.063 . 1 259 82 54 LYS N N 117.973 . 1 260 83 55 ARG H H 7.152 . 1 261 83 55 ARG HA H 3.089 . 1 262 83 55 ARG CA C 59.477 . 1 263 83 55 ARG CB C 29.781 . 1 264 83 55 ARG N N 123.340 . 1 265 84 56 LYS H H 7.499 . 1 266 84 56 LYS HA H 3.764 . 1 267 84 56 LYS CA C 59.641 . 1 268 84 56 LYS CB C 31.826 . 1 269 84 56 LYS N N 118.510 . 1 270 85 57 GLU H H 7.698 . 1 271 85 57 GLU HA H 4.345 . 1 272 85 57 GLU CA C 59.048 . 1 273 85 57 GLU CB C 30.156 . 1 274 85 57 GLU N N 116.424 . 1 275 86 58 LYS H H 7.906 . 1 276 86 58 LYS HA H 4.289 . 1 277 86 58 LYS CA C 57.794 . 1 278 86 58 LYS CB C 30.924 . 1 279 86 58 LYS N N 120.976 . 1 280 87 59 VAL H H 8.491 . 1 281 87 59 VAL HA H 3.651 . 1 282 87 59 VAL HB H 2.113 . 1 283 87 59 VAL CA C 66.372 . 1 284 87 59 VAL CB C 31.782 . 1 285 87 59 VAL N N 121.284 . 1 286 88 60 GLU H H 8.144 . 1 287 88 60 GLU HA H 4.064 . 1 288 88 60 GLU CA C 59.269 . 1 289 88 60 GLU CB C 29.217 . 1 290 88 60 GLU N N 120.018 . 1 291 89 61 GLU H H 7.976 . 1 292 89 61 GLU HA H 4.139 . 1 293 89 61 GLU CA C 58.888 . 1 294 89 61 GLU CB C 30.041 . 1 295 89 61 GLU N N 119.297 . 1 296 90 62 THR H H 7.816 . 1 297 90 62 THR HA H 4.326 . 1 298 90 62 THR HB H 4.176 . 1 299 90 62 THR CA C 65.117 . 1 300 90 62 THR CB C 69.298 . 1 301 90 62 THR N N 112.852 . 1 302 91 63 GLU H H 7.925 . 1 303 91 63 GLU HA H 4.101 . 1 304 91 63 GLU CA C 58.274 . 1 305 91 63 GLU CB C 29.733 . 1 306 91 63 GLU N N 121.224 . 1 307 92 64 LEU H H 7.806 . 1 308 92 64 LEU HA H 4.139 . 1 309 92 64 LEU CA C 56.862 . 1 310 92 64 LEU CB C 41.952 . 1 311 92 64 LEU N N 120.317 . 1 312 93 65 TYR H H 7.782 . 1 313 93 65 TYR HA H 4.364 . 1 314 93 65 TYR CA C 59.157 . 1 315 93 65 TYR CB C 38.141 . 1 316 93 65 TYR N N 118.117 . 1 317 94 66 LEU H H 7.880 . 1 318 94 66 LEU HA H 4.176 . 1 319 94 66 LEU CA C 56.315 . 1 320 94 66 LEU CB C 42.259 . 1 321 94 66 LEU N N 120.907 . 1 322 95 67 ASN H H 8.079 . 1 323 95 67 ASN HA H 4.626 . 1 324 95 67 ASN CA C 53.874 . 1 325 95 67 ASN CB C 38.588 . 1 326 95 67 ASN N N 117.450 . 1 327 96 68 LEU H H 7.946 . 1 328 96 68 LEU HA H 4.251 . 1 329 96 68 LEU CA C 55.975 . 1 330 96 68 LEU CB C 42.263 . 1 331 96 68 LEU N N 121.130 . 1 332 97 69 GLU H H 8.127 . 1 333 97 69 GLU HA H 4.176 . 1 334 97 69 GLU CA C 56.969 . 1 335 97 69 GLU CB C 29.997 . 1 336 97 69 GLU N N 119.590 . 1 337 98 70 ARG H H 7.950 . 1 338 98 70 ARG HA H 4.251 . 1 339 98 70 ARG CA C 56.022 . 1 340 98 70 ARG CB C 30.536 . 1 341 98 70 ARG N N 120.021 . 1 342 99 71 ILE H H 7.976 . 1 343 99 71 ILE CA C 58.954 . 1 344 99 71 ILE CB C 38.288 . 1 345 99 71 ILE N N 122.825 . 1 346 100 72 PRO HA H 4.364 . 1 347 100 72 PRO CA C 63.604 . 1 348 100 72 PRO CB C 31.793 . 1 349 101 73 TRP H H 7.849 . 1 350 101 73 TRP HA H 4.626 . 1 351 101 73 TRP CA C 57.052 . 1 352 101 73 TRP CB C 29.368 . 1 353 101 73 TRP N N 120.483 . 1 354 102 74 VAL H H 7.690 . 1 355 102 74 VAL HA H 3.989 . 1 356 102 74 VAL HB H 1.888 . 1 357 102 74 VAL CA C 62.197 . 1 358 102 74 VAL N N 122.073 . 1 359 103 75 VAL H H 8.068 . 1 360 103 75 VAL HA H 3.951 . 1 361 103 75 VAL HB H 1.963 . 1 362 103 75 VAL CA C 62.279 . 1 363 103 75 VAL CB C 32.627 . 1 364 103 75 VAL N N 124.564 . 1 365 104 76 ARG H H 8.299 . 1 366 104 76 ARG HA H 4.326 . 1 367 104 76 ARG CA C 55.977 . 1 368 104 76 ARG CB C 30.803 . 1 369 104 76 ARG N N 126.441 . 1 370 105 77 LYS H H 7.967 . 1 371 105 77 LYS CA C 57.824 . 1 372 105 77 LYS CB C 33.685 . 1 373 105 77 LYS N N 128.702 . 1 stop_ save_ save_heteronuclear_noe_list_1 _Saveframe_category heteronuclear_NOE _Details . loop_ _Experiment_label '2D 1H-15N HeteroNuclear NOE' stop_ loop_ _Sample_label $cchfv_gn_729-805_n15 stop_ _Sample_conditions_label $sample_conditions_1 _Spectrometer_frequency_1H 800 _Mol_system_component_name 'Crimean Congo Hemorrhagic Fever Virus Cytoplasmic Gn Tail' _Atom_one_atom_name N _Atom_two_atom_name H _Heteronuclear_NOE_value_type 'peak height' _NOE_reference_value 2.00E+07 _NOE_reference_description . _Text_data_format . _Text_data . loop_ _Residue_seq_code _Residue_label _Heteronuclear_NOE_value _Heteronuclear_NOE_value_error 2 GLU 0.314885 0.007791 3 LEU 0.337778 0.005238 4 LYS 0.397146 0.005006 6 GLN 0.558482 0.007782 7 THR 0.671959 0.008726 8 CYS 0.818975 0.016732 9 THR 0.778902 0.009333 10 ILE 0.730622 0.011674 11 CYS 0.791074 0.013573 12 GLU 0.761765 0.013422 13 THR 0.779868 0.015543 16 VAL 0.736633 0.029155 17 ASN 0.811933 0.014242 19 ILE 0.728276 0.01076 20 ASP 0.76649 0.010817 21 ALA 0.814764 0.01176 22 GLU 0.773142 0.010276 23 MET 0.797691 0.012848 25 ASP 0.816631 0.014389 26 LEU 0.822705 0.013212 27 ASN 0.773483 0.012679 28 CYS 0.779951 0.017274 29 SER 0.771414 0.01183 30 TYR 0.780715 0.01268 31 ASN 0.72397 0.014195 32 ILE 0.78879 0.010844 33 CYS 0.842718 0.013762 35 TYR 0.816215 0.013358 36 CYS 0.836264 0.011898 37 ALA 0.81586 0.011043 38 SER 0.817122 0.009395 39 ARG 0.79502 0.008619 40 LEU 0.759687 0.012027 41 THR 0.751378 0.013473 43 ASP 0.674545 0.020752 44 GLY 0.725566 0.011542 45 LEU 0.823391 0.014815 46 ALA 0.75771 0.012877 47 ARG 0.735292 0.012662 48 HIS 0.806306 0.009634 49 VAL 0.824354 0.018479 50 THR 0.770478 0.011537 51 GLN 0.791531 0.011549 52 CYS 0.793321 0.007436 54 LYS 0.843007 0.011807 55 ARG 0.839926 0.014041 56 LYS 0.757106 0.014241 57 GLU 0.814679 0.017352 58 LYS 0.77497 0.012919 59 VAL 0.765967 0.009342 60 GLU 0.734194 0.012866 61 GLU 0.70307 0.012823 62 THR 0.723212 0.012905 63 GLU 0.811846 0.01424 64 LEU 0.193892 0.019365 65 TYR 0.653802 0.010594 66 LEU 0.597994 0.009817 67 ASN 0.541111 0.012202 68 LEU 0.551411 0.00749 69 GLU 0.484799 0.00736 70 ARG 0.486439 0.011045 71 ILE 0.495516 0.007756 73 TRP 0.552648 0.005686 74 VAL 0.380906 0.005748 75 VAL 0.303109 0.00482 76 ARG 0.149804 0.006955 77 LYS -0.1513 -0.003044 stop_ save_