data_17385 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; TEX13A RanBP2 zinc finger backbone chemical shifts ; _BMRB_accession_number 17385 _BMRB_flat_file_name bmr17385.str _Entry_type original _Submission_date 2010-12-23 _Accession_date 2010-12-23 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Mansfield Robyn E. . 2 Mackay Joel P. . 3 Nguyen Cuong D. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 33 "13C chemical shifts" 71 "15N chemical shifts" 33 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2011-03-24 update BMRB 'update entry citation' 2011-01-28 original author 'original release' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Characterization of a Family of RanBP2-Type Zinc Fingers that Can Recognize Single-Stranded RNA.' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 21256132 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Nguyen Cuong D. . 2 Mansfield Robyn E. . 3 Leung Wilfred . . 4 Vaz Paula M. . 5 Loughlin Fionna E. . 6 Grant Richard P. . 7 Mackay Joel P. . stop_ _Journal_abbreviation 'J. Mol. Biol.' _Journal_name_full 'Journal of molecular biology' _Journal_volume 407 _Journal_issue 2 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 273 _Page_last 283 _Year 2011 _Details . loop_ _Keyword RanBP2 RNA-binding stop_ save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'TEX13A ZnF' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'protein component' $TEX13A_ZnF 'zinc ion' $ZN stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . loop_ _Biological_function RNA-binding stop_ _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_TEX13A_ZnF _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common TEX13A_ZnF _Molecular_mass . _Mol_thiol_state 'all other bound' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 42 _Mol_residue_sequence ; GSRPPVYRRPGDWDCPWCNA VNFSRRDTCFDCGKGIWLQK PH ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 368 GLY 2 369 SER 3 370 ARG 4 371 PRO 5 372 PRO 6 373 VAL 7 374 TYR 8 375 ARG 9 376 ARG 10 377 PRO 11 378 GLY 12 379 ASP 13 380 TRP 14 381 ASP 15 382 CYS 16 383 PRO 17 384 TRP 18 385 CYS 19 386 ASN 20 387 ALA 21 388 VAL 22 389 ASN 23 390 PHE 24 391 SER 25 392 ARG 26 393 ARG 27 394 ASP 28 395 THR 29 396 CYS 30 397 PHE 31 398 ASP 32 399 CYS 33 400 GLY 34 401 LYS 35 402 GLY 36 403 ILE 37 404 TRP 38 405 LEU 39 406 GLN 40 407 LYS 41 408 PRO 42 409 HIS stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-07-08 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value GB AAH42547 "TEX13A protein [Homo sapiens]" 95.24 409 100.00 100.00 2.77e-22 GB AAI08735 "Testis expressed 13A [Homo sapiens]" 95.24 409 100.00 100.00 2.77e-22 GB AAK31976 "testis protein TEX13A [Homo sapiens]" 95.24 409 100.00 100.00 2.77e-22 GB ABZ92542 "testis expressed 13A [synthetic construct]" 95.24 409 100.00 100.00 2.77e-22 GB ADZ15641 "testis expressed 13A [synthetic construct]" 95.24 409 100.00 100.00 2.77e-22 REF NP_001278206 "testis-expressed sequence 13A protein [Homo sapiens]" 95.24 409 100.00 100.00 2.77e-22 REF NP_112564 "testis-expressed sequence 13A protein [Homo sapiens]" 95.24 409 100.00 100.00 2.77e-22 REF XP_001092375 "PREDICTED: testis-expressed sequence 13A protein-like [Macaca mulatta]" 95.24 409 97.50 100.00 5.72e-22 REF XP_002831997 "PREDICTED: testis-expressed sequence 13A protein [Pongo abelii]" 95.24 410 97.50 100.00 1.23e-21 REF XP_003317653 "PREDICTED: testis-expressed sequence 13A protein [Pan troglodytes]" 95.24 409 97.50 100.00 3.19e-22 SP Q9BXU3 "RecName: Full=Testis-expressed sequence 13A protein" 95.24 409 100.00 100.00 2.77e-22 stop_ save_ ############# # Ligands # ############# save_ZN _Saveframe_category ligand _Mol_type non-polymer _Name_common "ZN (ZINC ION)" _BMRB_code . _PDB_code ZN _Molecular_mass 65.409 _Mol_charge 2 _Mol_paramagnetic . _Mol_aromatic no _Details ; Information obtained from PDB's Chemical Component Dictionary at http://wwpdb-remediation.rutgers.edu/downloads.html Downloaded on Fri Dec 9 11:09:24 2011 ; loop_ _Atom_name _PDB_atom_name _Atom_type _Atom_chirality _Atom_charge _Atom_oxidation_number _Atom_unpaired_electrons ZN ZN ZN . 2 . ? stop_ _Mol_thiol_state . _Sequence_homology_query_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $TEX13A_ZnF 'E. coli' 562 Bacteria . Escherichia coli stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $TEX13A_ZnF 'recombinant technology' . Escherichia coli . pGEX-2T stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $TEX13A_ZnF 1 mM '[U-100% 13C; U-100% 15N]' DTT 1 mM 'natural abundance' 'sodium phosphate' 25 mM 'natural abundance' 'sodium chloride' 100 mM 'natural abundance' DSS 20 uM 'natural abundance' H2O 93 % 'natural abundance' D2O 7 % 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_TOPSPIN _Saveframe_category software _Name TOPSPIN _Version . loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . stop_ loop_ _Task collection processing stop_ _Details . save_ save_SPARKY _Saveframe_category software _Name SPARKY _Version . loop_ _Vendor _Address _Electronic_address Goddard . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DRX _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_2D_1H-13C_HSQC_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HSQC' _Sample_label $sample_1 save_ save_3D_CBCA(CO)NH_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $sample_1 save_ save_3D_HNCACB_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ save_NMR_spectrometer_expt _Saveframe_category NMR_applied_experiment _Experiment_name . _BMRB_pulse_sequence_accession_number . _Details . save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.125 . M pH 6.5 . pH pressure 1 . atm temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio _Indirect_shift_ratio_citation_label _Correction_value_citation_label DSS C 13 'methyl protons' ppm 0.00 . indirect . . . 0.251449530 $entry_citation $entry_citation DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 $entry_citation $entry_citation DSS N 15 'methyl protons' ppm 0.00 . indirect . . . 0.101329118 $entry_citation $entry_citation stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' '3D CBCA(CO)NH' '3D HNCACB' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'protein component' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 372 5 PRO CA C 62.889 0.037 1 2 372 5 PRO CB C 31.999 0.065 1 3 373 6 VAL H H 8.064 0.008 1 4 373 6 VAL CA C 62.096 0.008 1 5 373 6 VAL CB C 33.083 0.005 1 6 373 6 VAL N N 120.184 0.024 1 7 374 7 TYR H H 8.272 0.020 1 8 374 7 TYR CA C 57.618 0.091 1 9 374 7 TYR CB C 38.857 0.043 1 10 374 7 TYR N N 125.060 0.029 1 11 375 8 ARG H H 8.118 0.006 1 12 375 8 ARG CA C 55.630 0.046 1 13 375 8 ARG CB C 31.732 0.019 1 14 375 8 ARG N N 124.895 0.023 1 15 376 9 ARG H H 8.808 0.007 1 16 376 9 ARG CA C 54.011 0.000 1 17 376 9 ARG CB C 30.007 0.000 1 18 376 9 ARG N N 126.398 0.013 1 19 378 11 GLY CA C 45.275 0.025 1 20 379 12 ASP H H 7.949 0.001 1 21 379 12 ASP CA C 55.954 0.116 1 22 379 12 ASP CB C 40.896 0.004 1 23 379 12 ASP N N 123.361 0.012 1 24 380 13 TRP H H 8.757 0.004 1 25 380 13 TRP CA C 54.080 0.035 1 26 380 13 TRP CB C 31.540 0.035 1 27 380 13 TRP N N 119.957 0.013 1 28 381 14 ASP H H 8.613 0.006 1 29 381 14 ASP CA C 52.887 0.030 1 30 381 14 ASP CB C 41.821 0.010 1 31 381 14 ASP N N 120.981 0.042 1 32 382 15 CYS H H 9.357 0.003 1 33 382 15 CYS CA C 57.211 0.000 1 34 382 15 CYS CB C 31.804 0.000 1 35 382 15 CYS N N 129.411 0.029 1 36 383 16 PRO CA C 63.986 0.002 1 37 383 16 PRO CB C 31.788 0.027 1 38 384 17 TRP H H 9.173 0.001 1 39 384 17 TRP CA C 59.023 0.108 1 40 384 17 TRP CB C 30.152 0.018 1 41 384 17 TRP N N 122.938 0.031 1 42 385 18 CYS H H 8.842 0.005 1 43 385 18 CYS CA C 58.862 0.099 1 44 385 18 CYS CB C 32.994 0.009 1 45 385 18 CYS N N 119.955 0.044 1 46 386 19 ASN H H 8.022 0.001 1 47 386 19 ASN CA C 55.898 0.040 1 48 386 19 ASN CB C 38.031 0.010 1 49 386 19 ASN N N 117.525 0.026 1 50 387 20 ALA H H 8.843 0.002 1 51 387 20 ALA CA C 53.802 0.035 1 52 387 20 ALA CB C 20.650 0.006 1 53 387 20 ALA N N 126.757 0.017 1 54 388 21 VAL H H 8.396 0.005 1 55 388 21 VAL CA C 62.622 0.036 1 56 388 21 VAL CB C 31.724 0.068 1 57 388 21 VAL N N 122.231 0.032 1 58 389 22 ASN H H 9.034 0.006 1 59 389 22 ASN CA C 52.263 0.055 1 60 389 22 ASN CB C 43.539 0.032 1 61 389 22 ASN N N 126.713 0.025 1 62 390 23 PHE H H 7.902 0.006 1 63 390 23 PHE CA C 56.623 0.058 1 64 390 23 PHE CB C 39.974 0.097 1 65 390 23 PHE N N 118.875 0.055 1 66 391 24 SER H H 8.440 0.002 1 67 391 24 SER CA C 60.747 0.049 1 68 391 24 SER CB C 63.501 0.038 1 69 391 24 SER N N 117.949 0.036 1 70 392 25 ARG H H 6.893 0.004 1 71 392 25 ARG CA C 56.634 0.071 1 72 392 25 ARG CB C 29.608 0.006 1 73 392 25 ARG N N 116.729 0.032 1 74 393 26 ARG H H 8.346 0.004 1 75 393 26 ARG CA C 56.984 0.089 1 76 393 26 ARG CB C 32.135 0.023 1 77 393 26 ARG N N 120.498 0.022 1 78 394 27 ASP H H 8.634 0.003 1 79 394 27 ASP CA C 53.934 0.095 1 80 394 27 ASP CB C 41.462 0.027 1 81 394 27 ASP N N 118.894 0.039 1 82 395 28 THR H H 7.699 0.002 1 83 395 28 THR CA C 59.508 0.067 1 84 395 28 THR CB C 72.632 0.005 1 85 395 28 THR N N 110.560 0.037 1 86 396 29 CYS H H 8.673 0.004 1 87 396 29 CYS CA C 58.788 0.046 1 88 396 29 CYS CB C 31.588 0.008 1 89 396 29 CYS N N 127.058 0.027 1 90 397 30 PHE H H 9.518 0.007 1 91 397 30 PHE CA C 60.245 0.031 1 92 397 30 PHE CB C 38.601 0.020 1 93 397 30 PHE N N 131.382 0.033 1 94 398 31 ASP H H 8.533 0.006 1 95 398 31 ASP CA C 55.562 0.076 1 96 398 31 ASP CB C 42.745 0.019 1 97 398 31 ASP N N 125.099 0.051 1 98 399 32 CYS H H 8.380 0.001 1 99 399 32 CYS CA C 59.354 0.051 1 100 399 32 CYS CB C 31.856 0.015 1 101 399 32 CYS N N 117.892 0.038 1 102 400 33 GLY H H 7.457 0.002 1 103 400 33 GLY CA C 46.328 0.011 1 104 400 33 GLY N N 111.729 0.032 1 105 401 34 LYS H H 8.206 0.001 1 106 401 34 LYS CA C 57.282 0.023 1 107 401 34 LYS CB C 33.326 0.048 1 108 401 34 LYS N N 121.968 0.032 1 109 402 35 GLY H H 8.275 0.006 1 110 402 35 GLY CA C 44.754 0.004 1 111 402 35 GLY N N 110.163 0.028 1 112 403 36 ILE H H 7.957 0.004 1 113 403 36 ILE CA C 62.152 0.028 1 114 403 36 ILE CB C 38.597 0.063 1 115 403 36 ILE N N 120.972 0.016 1 116 404 37 TRP H H 7.333 0.004 1 117 404 37 TRP CA C 57.102 0.076 1 118 404 37 TRP CB C 28.630 0.052 1 119 404 37 TRP N N 119.706 0.105 1 120 405 38 LEU H H 7.461 0.002 1 121 405 38 LEU CA C 54.957 0.040 1 122 405 38 LEU CB C 42.017 0.015 1 123 405 38 LEU N N 124.331 0.015 1 124 406 39 GLN H H 8.201 0.007 1 125 406 39 GLN CA C 55.592 0.043 1 126 406 39 GLN CB C 29.634 0.007 1 127 406 39 GLN N N 121.660 0.022 1 128 407 40 LYS H H 8.308 0.010 1 129 407 40 LYS CA C 54.300 0.000 1 130 407 40 LYS CB C 32.519 0.000 1 131 407 40 LYS N N 124.786 0.020 1 132 408 41 PRO CA C 63.468 0.014 1 133 408 41 PRO CB C 31.931 0.001 1 134 409 42 HIS H H 7.894 0.002 1 135 409 42 HIS CA C 57.375 0.000 1 136 409 42 HIS CB C 30.498 0.000 1 137 409 42 HIS N N 124.164 0.018 1 stop_ save_