data_17460 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; The Stability and Dynamics of Ribosomal Protein L9: Investigations of a Molecular Strut by Amide Proton Exchange and Circular Dichroism ; _BMRB_accession_number 17460 _BMRB_flat_file_name bmr17460.str _Entry_type original _Submission_date 2011-02-11 _Accession_date 2011-02-11 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Lillemoen Jarle . . 2 Cameron Christopher S. . 3 Hoffman David W. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count chemical_rates 1 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2012-06-06 original author . stop_ _Original_release_date 2012-06-06 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'The Stability and Dynamics of Ribosomal Protein L9: Investigations of a Molecular Strut by Amide Proton Exchange and Circular Dichroism' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Lillemoen Jarle . . 2 Cameron Christopher S. . 3 Hoffman David W. . stop_ _Journal_abbreviation 'J. Mol. Biol.' _Journal_volume 268 _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 482 _Page_last 493 _Year 1997 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name L9 _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label L9 $L9 stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_L9 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common L9 _Molecular_mass . _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 149 _Mol_residue_sequence ; MKVIFLKDVKGKGKKGEIKN VADGYANNFLFKQGLAIEAT PANLKALEAQKQKEQRQAAE ELANAKKLKEQLEKLTVTIP AKAGEGGRLFGSITSKQIAE SLQAQHGLKLDKRKIELADA IRALGYTNVPVKLHPEVTAT LKVHVTEQK ; loop_ _Residue_seq_code _Residue_label 1 MET 2 LYS 3 VAL 4 ILE 5 PHE 6 LEU 7 LYS 8 ASP 9 VAL 10 LYS 11 GLY 12 LYS 13 GLY 14 LYS 15 LYS 16 GLY 17 GLU 18 ILE 19 LYS 20 ASN 21 VAL 22 ALA 23 ASP 24 GLY 25 TYR 26 ALA 27 ASN 28 ASN 29 PHE 30 LEU 31 PHE 32 LYS 33 GLN 34 GLY 35 LEU 36 ALA 37 ILE 38 GLU 39 ALA 40 THR 41 PRO 42 ALA 43 ASN 44 LEU 45 LYS 46 ALA 47 LEU 48 GLU 49 ALA 50 GLN 51 LYS 52 GLN 53 LYS 54 GLU 55 GLN 56 ARG 57 GLN 58 ALA 59 ALA 60 GLU 61 GLU 62 LEU 63 ALA 64 ASN 65 ALA 66 LYS 67 LYS 68 LEU 69 LYS 70 GLU 71 GLN 72 LEU 73 GLU 74 LYS 75 LEU 76 THR 77 VAL 78 THR 79 ILE 80 PRO 81 ALA 82 LYS 83 ALA 84 GLY 85 GLU 86 GLY 87 GLY 88 ARG 89 LEU 90 PHE 91 GLY 92 SER 93 ILE 94 THR 95 SER 96 LYS 97 GLN 98 ILE 99 ALA 100 GLU 101 SER 102 LEU 103 GLN 104 ALA 105 GLN 106 HIS 107 GLY 108 LEU 109 LYS 110 LEU 111 ASP 112 LYS 113 ARG 114 LYS 115 ILE 116 GLU 117 LEU 118 ALA 119 ASP 120 ALA 121 ILE 122 ARG 123 ALA 124 LEU 125 GLY 126 TYR 127 THR 128 ASN 129 VAL 130 PRO 131 VAL 132 LYS 133 LEU 134 HIS 135 PRO 136 GLU 137 VAL 138 THR 139 ALA 140 THR 141 LEU 142 LYS 143 VAL 144 HIS 145 VAL 146 THR 147 GLU 148 GLN 149 LYS stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-09-30 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 15883 CTL9 61.74 92 100.00 100.00 1.22e-55 BMRB 15884 CTL9 61.74 92 100.00 100.00 1.22e-55 PDB 1DIV "Ribosomal Protein L9" 100.00 149 100.00 100.00 9.95e-99 PDB 1GIY "Crystal Structure Of The Ribosome At 5.5 A Resolution. This File, 1giy, Contains The 50s Ribosome Subunit. The 30s Ribosome Sub" 100.00 149 100.00 100.00 9.95e-99 PDB 1YL3 "Crystal Structure Of 70s Ribosome With Thrs Operator And Trnas. Large Subunit. The Coordinates For The Small Subunit Are In The" 100.00 149 100.00 100.00 9.95e-99 PDB 2B66 "50s Ribosomal Subunit From A Crystal Structure Of Release Factor Rf1, Trnas And Mrna Bound To The Ribosome. This File Contains " 100.00 149 100.00 100.00 9.95e-99 PDB 2B9N "50s Ribosomal Subunit From A Crystal Structure Of Release Factor Rf2, Trnas And Mrna Bound To The Ribosome. This File Contains " 100.00 149 100.00 100.00 9.95e-99 PDB 2B9P "50s Ribosomal Subunit From A Crystal Structure Of The Ribosome In Complex With Trnas And Mrna With A Stop Codon In The A-Site. " 100.00 149 100.00 100.00 9.95e-99 PDB 487D "Seven Ribosomal Proteins Fitted To A Cryo-Electron Microscopic Map Of The Large 50s Subunit At 7.5 Angstroms Resolution" 100.00 149 100.00 100.00 9.95e-99 EMBL CAA43972 "ribosomal protein L9 [Geobacillus stearothermophilus]" 99.33 148 100.00 100.00 2.81e-98 GB AAA22701 "ribosomal protein L9 [Geobacillus stearothermophilus]" 100.00 149 99.33 99.33 6.37e-98 GB AKM20686 "50S ribosomal protein L9 [Geobacillus sp. 12AMOR1]" 100.00 149 100.00 100.00 9.95e-99 GB AKU26730 "50S ribosomal protein L9 [Geobacillus sp. LC300]" 100.00 149 99.33 99.33 4.03e-98 GB KFL16938 "50S ribosomal protein L9 [Geobacillus stearothermophilus]" 100.00 149 100.00 100.00 9.95e-99 GB KFX34457 "50S ribosomal protein L9 [Geobacillus stearothermophilus]" 100.00 149 100.00 100.00 9.95e-99 PRF 0701226A "ribosomal protein L17" 100.00 147 98.66 98.66 1.81e-93 PRF 1714237A "ribosomal protein L9" 99.33 148 100.00 100.00 2.81e-98 REF WP_033008865 "MULTISPECIES: 50S ribosomal protein L9 [Bacillaceae]" 100.00 149 100.00 100.00 9.95e-99 REF WP_050368025 "50S ribosomal protein L9 [Geobacillus sp. LC300]" 100.00 149 99.33 99.33 4.03e-98 SP P02417 "RecName: Full=50S ribosomal protein L9; AltName: Full=BL17" 100.00 149 100.00 100.00 9.95e-99 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $L9 'B. stearothermophilus' 1422 Bacteria . Geobacillus stearothermophilus stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $L9 'recombinant technology' . Escherichia coli . ns stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $L9 ns mM '[U-100% 15N]' D2O 100 % 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_MOLSCRIPT _Saveframe_category software _Name MOLSCRIPT _Version . loop_ _Vendor _Address _Electronic_address 'Kraulis, 1991' . . stop_ loop_ _Task 'ribbon drawing' stop_ _Details . save_ save_MIDAS _Saveframe_category software _Name MIDAS _Version . loop_ _Vendor _Address _Electronic_address 'Ferrin et al., 1988; Huang et al., 1991' . . stop_ loop_ _Task 'figure representation' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model INOVA _Field_strength 500 _Details . save_ ############################# # NMR applied experiments # ############################# save_1H-2H_HSMQC_exchange_1 _Saveframe_category NMR_applied_experiment _Experiment_name '1H-2H HSMQC exchange' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 7.0 . pH pressure 1 . atm temperature 305 . K stop_ save_