data_17626 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Backbone 1H, 13C, and 15N Chemical Shift Assignments for Act2-EF34 in complex with palladin peptide ; _BMRB_accession_number 17626 _BMRB_flat_file_name bmr17626.str _Entry_type original _Submission_date 2011-05-05 _Accession_date 2011-05-05 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Beck Moriah R. . 2 Campbell Sharon L. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 52 "13C chemical shifts" 103 "15N chemical shifts" 54 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2012-03-07 update BMRB 'update entry citation' 2011-10-12 original author 'original release' stop_ loop_ _Related_BMRB_accession_number _Relationship 4453 Act2EF34-Zr7 4454 Act2EF34-Zr7 17627 'apo actinin-2 C-terminal EF-hand (Act2-EF34)' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Structural characterization of the interactions between palladin and -actinin.' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 21925511 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Beck Moriah R. . 2 Otey Carol A. . 3 Campbell Sharon L. . stop_ _Journal_abbreviation 'J. Mol. Biol.' _Journal_name_full 'Journal of molecular biology' _Journal_volume 413 _Journal_issue 3 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 712 _Page_last 725 _Year 2011 _Details . loop_ _Keyword palladin alpha-actinin 'actin cytoskeleton' stop_ save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'Act2-EF34-palladin complex' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label protein $Act2-EF34 ligand $palladin stop_ _System_molecular_weight 9848 _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_Act2-EF34 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common Act2-EF34 _Molecular_mass 8071.05 _Mol_thiol_state 'all free' loop_ _Biological_function 'F-actin crosslinking protein' stop_ _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 75 _Mol_residue_sequence ; GAMADTDTAEQVIASFRILA SDKPYILAEELRRELPPDQA QYCIKRMPAYSGPGSVPGAL DYAAFSSALYGESDL ; loop_ _Residue_seq_code _Residue_label 1 GLY 2 ALA 3 MET 4 ALA 5 ASP 6 THR 7 ASP 8 THR 9 ALA 10 GLU 11 GLN 12 VAL 13 ILE 14 ALA 15 SER 16 PHE 17 ARG 18 ILE 19 LEU 20 ALA 21 SER 22 ASP 23 LYS 24 PRO 25 TYR 26 ILE 27 LEU 28 ALA 29 GLU 30 GLU 31 LEU 32 ARG 33 ARG 34 GLU 35 LEU 36 PRO 37 PRO 38 ASP 39 GLN 40 ALA 41 GLN 42 TYR 43 CYS 44 ILE 45 LYS 46 ARG 47 MET 48 PRO 49 ALA 50 TYR 51 SER 52 GLY 53 PRO 54 GLY 55 SER 56 VAL 57 PRO 58 GLY 59 ALA 60 LEU 61 ASP 62 TYR 63 ALA 64 ALA 65 PHE 66 SER 67 SER 68 ALA 69 LEU 70 TYR 71 GLY 72 GLU 73 SER 74 ASP 75 LEU stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-02-05 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 17627 Act2-EF34 100.00 75 100.00 100.00 4.17e-45 BMRB 4453 "alpha-actinin C-terminal EF-hands" 100.00 75 100.00 100.00 4.17e-45 BMRB 4454 "alpha-actinin C-terminal EF-hands" 100.00 75 100.00 100.00 4.17e-45 PDB 1H8B "Ef-Hands 3,4 From Alpha-Actinin Z-Repeat 7 From Titin" 100.00 75 100.00 100.00 4.17e-45 PDB 4D1E "The Crystal Structure Of Human Muscle Alpha-actinin-2" 96.00 876 100.00 100.00 7.65e-41 DBJ BAB22865 "unnamed protein product [Mus musculus]" 96.00 225 97.22 97.22 1.70e-41 DBJ BAD92758 "actinin, alpha 2 variant [Homo sapiens]" 96.00 664 100.00 100.00 1.07e-40 DBJ BAG37672 "unnamed protein product [Homo sapiens]" 96.00 894 100.00 100.00 7.52e-41 DBJ BAH11921 "unnamed protein product [Homo sapiens]" 96.00 803 98.61 98.61 1.84e-40 DBJ BAH12587 "unnamed protein product [Homo sapiens]" 96.00 679 100.00 100.00 1.20e-40 EMBL CAB61269 "alpha-actinin 2 protein [Homo sapiens]" 96.00 894 100.00 100.00 6.75e-41 GB AAA51583 "alpha-actinin [Homo sapiens]" 96.00 894 100.00 100.00 6.75e-41 GB AAF76325 "alpha-actinin 2 [Mus musculus]" 96.00 894 97.22 97.22 1.12e-39 GB AAH47901 "Actinin, alpha 2 [Homo sapiens]" 96.00 894 100.00 100.00 6.75e-41 GB AAH51770 "Actinin, alpha 2 [Homo sapiens]" 96.00 894 100.00 100.00 6.75e-41 GB AAH89579 "Actinin alpha 2 [Mus musculus]" 96.00 894 97.22 97.22 1.20e-39 REF NP_001029807 "alpha-actinin-2 [Bos taurus]" 96.00 894 98.61 98.61 2.41e-40 REF NP_001094 "alpha-actinin-2 isoform 1 [Homo sapiens]" 96.00 894 100.00 100.00 6.75e-41 REF NP_001163796 "alpha-actinin-2 [Rattus norvegicus]" 96.00 894 97.22 97.22 1.33e-39 REF NP_001230595 "alpha-actinin-2 [Sus scrofa]" 96.00 894 98.61 98.61 2.61e-40 REF NP_001265272 "alpha-actinin-2 isoform 2 [Homo sapiens]" 96.00 894 100.00 100.00 7.52e-41 SP P35609 "RecName: Full=Alpha-actinin-2; AltName: Full=Alpha-actinin skeletal muscle isoform 2; AltName: Full=F-actin cross-linking prote" 96.00 894 100.00 100.00 6.75e-41 SP Q3ZC55 "RecName: Full=Alpha-actinin-2; AltName: Full=Alpha-actinin skeletal muscle isoform 2; AltName: Full=F-actin cross-linking prote" 96.00 894 98.61 98.61 2.41e-40 SP Q9JI91 "RecName: Full=Alpha-actinin-2; AltName: Full=Alpha-actinin skeletal muscle isoform 2; AltName: Full=F-actin cross-linking prote" 96.00 894 97.22 97.22 1.33e-39 stop_ save_ save_palladin _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common palladin _Molecular_mass 1777 _Mol_thiol_state 'not present' loop_ _Biological_function 'Cytoskeletal protein required for organization of normal actin cytoskeleton; scaffolding protein' stop_ _Details . _Residue_count 17 _Mol_residue_sequence HGQTPAAFLSALLPSQP loop_ _Residue_seq_code _Residue_label 1 HIS 2 GLY 3 GLN 4 THR 5 PRO 6 ALA 7 ALA 8 PHE 9 LEU 10 SER 11 ALA 12 LEU 13 LEU 14 PRO 15 SER 16 GLN 17 PRO stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-01-30 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value DBJ BAA76836 "KIAA0992 protein [Homo sapiens]" 100.00 772 100.00 100.00 7.57e-01 DBJ BAG09933 "palladin [synthetic construct]" 100.00 672 100.00 100.00 6.14e-01 GB AAG00079 "90 kDa actin-associated protein palladin [Mus musculus]" 94.12 323 100.00 100.00 9.73e+00 GB EDL87206 "rCG59206 [Rattus norvegicus]" 94.12 580 100.00 100.00 6.50e+00 REF XP_004852973 "PREDICTED: palladin isoform X1 [Heterocephalus glaber]" 94.12 1370 100.00 100.00 6.22e+00 REF XP_004852974 "PREDICTED: palladin isoform X2 [Heterocephalus glaber]" 94.12 1154 100.00 100.00 7.16e+00 REF XP_005003446 "PREDICTED: LOW QUALITY PROTEIN: palladin [Cavia porcellus]" 94.12 1375 100.00 100.00 6.45e+00 REF XP_005369855 "PREDICTED: palladin isoform X1 [Microtus ochrogaster]" 94.12 1407 100.00 100.00 6.53e+00 REF XP_005369857 "PREDICTED: palladin isoform X3 [Microtus ochrogaster]" 94.12 1001 100.00 100.00 7.44e+00 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $Act2-EF34 human 9606 Eukaryota Metazoa Homo sapiens $palladin human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name _Details $Act2-EF34 'recombinant technology' . . . . pET9d 'N-terminal 6-His tag followed by TEV protease site' $palladin 'chemical synthesis' . . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $Act2-EF34 . mM 0.2 0.5 '[U-100% 15N]' $palladin . mM 0.5 2 'natural abundance' D2O 10 % . . 'natural abundance' 'sodium azide' 0.05 mM . . 'natural abundance' TCEP . mM 2 5 'natural abundance' MOPS 20 mM . . 'natural abundance' 'sodium chloride' 10 mM . . 'natural abundance' H2O 90 % . . 'natural abundance' stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $Act2-EF34 . mM 0.2 0.5 '[U-100% 13C; U-100% 15N]' $palladin . mM 0.5 2 'natural abundance' D2O 10 % . . 'natural abundance' 'sodium azide' 0.05 mM . . 'natural abundance' TCEP . mM 2 5 'natural abundance' MOPS 20 mM . . 'natural abundance' 'sodium chloride' 10 mM . . 'natural abundance' H2O 90 % . . 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_NMRDraw _Saveframe_category software _Name NMRDraw _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ save_NMRViewJ _Saveframe_category software _Name NMRViewJ _Version 8.1 loop_ _Vendor _Address _Electronic_address 'Johnson, One Moon Scientific' . . stop_ loop_ _Task 'peak picking' 'data analysis' 'chemical shift assignment' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model INOVA _Field_strength 600 _Details . save_ save_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model INOVA _Field_strength 700 _Details cryoprobe save_ save_spectrometer_3 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model INOVA _Field_strength 700 _Details 'without cryoprobe' save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_CBCA(CO)NH_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $sample_2 save_ save_3D_HNCO_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO' _Sample_label $sample_2 save_ save_3D_HNCACB_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_2 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units temperature 300 . K pH 6.6 . pH pressure 1 . atm 'ionic strength' 0.01 . M stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS H 1 'methyl protons' ppm 0.00 internal direct . 'separate tube (no insert) similar to the experimental sample tube' . 1.000000000 DSS C 13 'methyl protons' ppm 0.00 . indirect . 'separate tube (no insert) similar to the experimental sample tube' . 0.251449530 DSS N 15 'methyl protons' ppm 0.00 . indirect . 'separate tube (no insert) similar to the experimental sample tube' . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' '3D CBCA(CO)NH' '3D HNCO' '3D HNCACB' stop_ loop_ _Sample_label $sample_1 $sample_2 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name protein _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 3 3 MET H H 8.48 . 1 2 3 3 MET CA C 55.62 . 1 3 3 3 MET CB C 32.9 . 1 4 3 3 MET N N 119.49 . 1 5 4 4 ALA H H 8.35 . 1 6 4 4 ALA CA C 52.64 . 1 7 4 4 ALA CB C 19.28 . 1 8 4 4 ALA N N 124.85 . 1 9 5 5 ASP H H 8.39 . 1 10 5 5 ASP CA C 54.65 . 1 11 5 5 ASP CB C 41.09 . 1 12 5 5 ASP N N 119.76 . 1 13 6 6 THR H H 8.08 . 1 14 6 6 THR CA C 62.6 . 1 15 6 6 THR CB C 70.22 . 1 16 6 6 THR N N 113.22 . 1 17 7 7 ASP H H 8.43 . 1 18 7 7 ASP CA C 55.13 . 1 19 7 7 ASP CB C 41.01 . 1 20 7 7 ASP N N 122.71 . 1 21 9 9 ALA H H 8.52 . 1 22 9 9 ALA N N 122.51 . 1 23 11 11 GLN H H 8.01 . 1 24 11 11 GLN CA C 58.75 . 1 25 11 11 GLN CB C 28.74 . 1 26 11 11 GLN N N 119.71 . 1 27 13 13 ILE H H 8.64 . 1 28 13 13 ILE CA C 67.81 . 1 29 13 13 ILE CB C 38.03 . 1 30 13 13 ILE N N 120.97 . 1 31 15 15 SER H H 7.81 . 1 32 15 15 SER CA C 61.96 . 1 33 15 15 SER CB C 64.95 . 1 34 15 15 SER N N 114.16 . 1 35 16 16 PHE CA C 55.93 . 1 36 16 16 PHE CB C 32.41 . 1 37 17 17 ARG H H 8.38 . 1 38 17 17 ARG CA C 60.48 . 1 39 17 17 ARG CB C 29.55 . 1 40 17 17 ARG N N 119.19 . 1 41 18 18 ILE H H 7.39 . 1 42 18 18 ILE CA C 65.14 . 1 43 18 18 ILE CB C 37.97 . 1 44 18 18 ILE N N 118.80 . 1 45 19 19 LEU H H 7.56 . 1 46 19 19 LEU CA C 54.37 . 1 47 19 19 LEU CB C 42.47 . 1 48 19 19 LEU N N 122.75 . 1 49 20 20 ALA H H 8.11 . 1 50 20 20 ALA CA C 51.46 . 1 51 20 20 ALA CB C 18.75 . 1 52 20 20 ALA N N 119.39 . 1 53 22 22 ASP H H 8.77 . 1 54 22 22 ASP CA C 56.15 . 1 55 22 22 ASP CB C 40.15 . 1 56 22 22 ASP N N 111.73 . 1 57 23 23 LYS H H 7.24 . 1 58 23 23 LYS CA C 56.15 . 1 59 23 23 LYS CB C 31.55 . 1 60 23 23 LYS N N 118.91 . 1 61 25 25 TYR H H 6.51 . 1 62 25 25 TYR CA C 54.73 . 1 63 25 25 TYR CB C 40.54 . 1 64 25 25 TYR N N 114.03 . 1 65 26 26 ILE H H 9.16 . 1 66 26 26 ILE CA C 59.12 . 1 67 26 26 ILE CB C 44.06 . 1 68 26 26 ILE N N 116.97 . 1 69 27 27 LEU H H 8.83 . 1 70 27 27 LEU CA C 53.24 . 1 71 27 27 LEU CB C 44.19 . 1 72 27 27 LEU N N 124.34 . 1 73 28 28 ALA H H 9.76 . 1 74 28 28 ALA CA C 56.39 . 1 75 28 28 ALA CB C 17.77 . 1 76 28 28 ALA N N 126.32 . 1 77 29 29 GLU H H 9.36 . 1 78 29 29 GLU CA C 59.82 . 1 79 29 29 GLU CB C 28.93 . 1 80 29 29 GLU N N 114.29 . 1 81 30 30 GLU H H 7.19 . 1 82 30 30 GLU CA C 59.11 . 1 83 30 30 GLU CB C 28.97 . 1 84 30 30 GLU N N 118.72 . 1 85 31 31 LEU H H 7.43 . 1 86 31 31 LEU CA C 58.73 . 1 87 31 31 LEU CB C 42.56 . 1 88 31 31 LEU N N 118.44 . 1 89 32 32 ARG H H 8.22 . 1 90 32 32 ARG CA C 58.72 . 1 91 32 32 ARG CB C 30.34 . 1 92 32 32 ARG N N 113.92 . 1 93 33 33 ARG H H 7.48 . 1 94 33 33 ARG CA C 58.75 . 1 95 33 33 ARG CB C 30.96 . 1 96 33 33 ARG N N 116.87 . 1 97 34 34 GLU H H 7.21 . 1 98 34 34 GLU CA C 56.57 . 1 99 34 34 GLU CB C 32.64 . 1 100 34 34 GLU N N 113.12 . 1 101 35 35 LEU H H 7.77 . 1 102 35 35 LEU CA C 51.88 . 1 103 35 35 LEU CB C 42.92 . 1 104 35 35 LEU N N 117.89 . 1 105 38 39 GLN H H 9.01 . 1 106 38 39 GLN CA C 57.62 . 1 107 38 39 GLN CB C 39.66 . 1 108 38 39 GLN N N 116.59 . 1 109 39 40 ALA H H 7.64 . 1 110 39 40 ALA CA C 59.09 . 1 111 39 40 ALA CB C 17.41 . 1 112 39 40 ALA N N 121.34 . 1 113 40 41 GLN H H 8.88 . 1 114 40 41 GLN CA C 56.53 . 1 115 40 41 GLN CB C 17.41 . 1 116 40 41 GLN N N 122.70 . 1 117 41 42 TYR H H 8.36 . 1 118 41 42 TYR CA C 58.89 . 1 119 41 42 TYR CB C 28.13 . 1 120 41 42 TYR N N 115.43 . 1 121 42 43 CYS H H 7.71 . 1 122 42 43 CYS N N 120.10 . 1 123 44 45 LYS H H 8.49 . 1 124 44 45 LYS CA C 66.09 . 1 125 44 45 LYS CB C 38.56 . 1 126 44 45 LYS N N 120.27 . 1 127 45 46 ARG H H 7.72 . 1 128 45 46 ARG CA C 57.29 . 1 129 45 46 ARG CB C 32.8 . 1 130 45 46 ARG N N 114.89 . 1 131 46 47 MET H H 7.27 . 1 132 46 47 MET CA C 56.16 . 1 133 46 47 MET CB C 32.24 . 1 134 46 47 MET N N 115.14 . 1 135 47 48 PRO CA C 56.99 . 1 136 47 48 PRO CB C 43.49 . 1 137 47 48 PRO N N 127.44 . 1 138 49 50 TYR H H 8.51 . 1 139 49 50 TYR CA C 52.81 . 1 140 49 50 TYR CB C 18.38 . 1 141 49 50 TYR N N 122.19 . 1 142 50 51 SER H H 8.31 . 1 143 50 51 SER CA C 57.69 . 1 144 50 51 SER CB C 38.96 . 1 145 50 51 SER N N 122.71 . 1 146 51 52 GLY H H 8.76 . 1 147 51 52 GLY CA C 56.73 . 1 148 51 52 GLY N N 124.49 . 1 149 54 56 VAL CA C 45.7 . 1 150 54 56 VAL CB C 60 . 1 151 55 57 PRO CA C 59.91 . 1 152 55 57 PRO CB C 63.17 . 1 153 55 57 PRO N N 111.80 . 1 154 56 58 GLY H H 6.29 . 1 155 56 58 GLY CA C 58.06 . 1 156 56 58 GLY N N 114.26 . 1 157 58 60 LEU H H 8.63 . 1 158 58 60 LEU CA C 44.8 . 1 159 58 60 LEU CB C 20 . 1 160 58 60 LEU N N 112.10 . 1 161 59 61 ASP H H 7.79 . 1 162 59 61 ASP CA C 53.72 . 1 163 59 61 ASP CB C 44.81 . 1 164 59 61 ASP N N 121.77 . 1 165 60 62 TYR H H 9.40 . 1 166 60 62 TYR CA C 54.58 . 1 167 60 62 TYR CB C 43.8 . 1 168 60 62 TYR N N 126.65 . 1 169 61 63 ALA H H 9.20 . 1 170 61 63 ALA CA C 52.29 . 1 171 61 63 ALA CB C 41.26 . 1 172 61 63 ALA N N 121.96 . 1 173 62 64 ALA H H 7.14 . 1 174 62 64 ALA CA C 58.71 . 1 175 62 64 ALA CB C 37.22 . 1 176 62 64 ALA N N 122.80 . 1 177 63 65 PHE H H 8.52 . 1 178 63 65 PHE N N 132.99 . 1 179 64 66 SER H H 7.90 . 1 180 64 66 SER CA C 54.63 . 1 181 64 66 SER CB C 18.56 . 1 182 64 66 SER N N 122.77 . 1 183 65 67 SER H H 7.35 . 1 184 65 67 SER CA C 59.97 . 1 185 65 67 SER CB C 41.59 . 1 186 65 67 SER N N 117.26 . 1 187 67 69 LEU H H 8.34 . 1 188 67 69 LEU CA C 59.59 . 1 189 67 69 LEU CB C 61.46 . 1 190 67 69 LEU N N 117.45 . 1 191 68 70 TYR H H 7.76 . 1 192 68 70 TYR CA C 58.31 . 1 193 68 70 TYR CB C 41.41 . 1 194 68 70 TYR N N 123.24 . 1 195 69 71 GLY H H 7.46 . 1 196 69 71 GLY CA C 57 . 1 197 69 71 GLY N N 118.07 . 1 198 71 73 SER H H 7.77 . 1 199 71 73 SER CA C 45.5 . 1 200 71 73 SER CB C 50 . 1 201 71 73 SER N N 109.22 . 1 202 72 74 ASP H H 8.35 . 1 203 72 74 ASP CA C 56.43 . 1 204 72 74 ASP CB C 30.45 . 1 205 72 74 ASP N N 120.38 . 1 206 73 75 LEU H H 8.35 . 1 207 73 75 LEU CA C 58.51 . 1 208 73 75 LEU CB C 64.47 . 1 209 73 75 LEU N N 117.00 . 1 stop_ save_