data_17672 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Backbone 1H, 13C, and 15N Chemical Shift Assignments for Human Just Another Zinc Finger Protein residues 228-277 ; _BMRB_accession_number 17672 _BMRB_flat_file_name bmr17672.str _Entry_type original _Submission_date 2011-05-28 _Accession_date 2011-05-28 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Burge Russell G. . 2 Martinez-Yamout Maria A. . 3 Dyson H. Jane . 4 Wright Peter E. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 31 "13C chemical shifts" 32 "15N chemical shifts" 31 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2014-02-26 update BMRB 'update entry citation' 2014-02-24 original author 'original release' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Structural Characterization of Interactions between the Double-Stranded RNA-Binding Zinc Finger Protein JAZ and Nucleic Acids.' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 24521053 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Burge Russell G. . 2 Martinez-Yamout Maria A. . 3 Dyson 'H. Jane' . . 4 Wright Peter E. . stop_ _Journal_abbreviation Biochemistry _Journal_name_full Biochemistry _Journal_volume . _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first . _Page_last . _Year 2014 _Details . loop_ _Keyword 'Just Another Zinc finger' stop_ save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'JAZ ZF4' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'JAZ ZF4' $JAZ_ZF4 Zinc $ZN stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_JAZ_ZF4 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common JAZ_ZF4 _Molecular_mass . _Mol_thiol_state 'all other bound' loop_ _Biological_function unknown stop_ _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 50 _Mol_residue_sequence ; PAVTDFPAGKGYPCKTCKIV LNSIEQYQAHVSGFKHKNQS PKTVASSLGQ ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 228 PRO 2 229 ALA 3 230 VAL 4 231 THR 5 232 ASP 6 233 PHE 7 234 PRO 8 235 ALA 9 236 GLY 10 237 LYS 11 238 GLY 12 239 TYR 13 240 PRO 14 241 CYS 15 242 LYS 16 243 THR 17 244 CYS 18 245 LYS 19 246 ILE 20 247 VAL 21 248 LEU 22 249 ASN 23 250 SER 24 251 ILE 25 252 GLU 26 253 GLN 27 254 TYR 28 255 GLN 29 256 ALA 30 257 HIS 31 258 VAL 32 259 SER 33 260 GLY 34 261 PHE 35 262 LYS 36 263 HIS 37 264 LYS 38 265 ASN 39 266 GLN 40 267 SER 41 268 PRO 42 269 LYS 43 270 THR 44 271 VAL 45 272 ALA 46 273 SER 47 274 SER 48 275 LEU 49 276 GLY 50 277 GLN stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-10-14 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value DBJ BAD18614 "unnamed protein product [Homo sapiens]" 100.00 319 100.00 100.00 6.74e-26 DBJ BAG72878 "zinc finger protein 346 [synthetic construct]" 100.00 294 100.00 100.00 4.04e-26 DBJ BAH12103 "unnamed protein product [Homo sapiens]" 100.00 262 100.00 100.00 3.72e-26 DBJ BAH12584 "unnamed protein product [Homo sapiens]" 100.00 196 100.00 100.00 5.42e-27 DBJ BAH12620 "unnamed protein product [Homo sapiens]" 86.00 118 100.00 100.00 7.09e-22 EMBL CAH18475 "hypothetical protein [Homo sapiens]" 100.00 294 100.00 100.00 4.04e-26 EMBL CAH93198 "hypothetical protein [Pongo abelii]" 100.00 310 100.00 100.00 4.79e-26 EMBL CAL37677 "hypothetical protein [synthetic construct]" 100.00 294 100.00 100.00 4.04e-26 EMBL CAL38643 "hypothetical protein, partial [synthetic construct]" 100.00 294 100.00 100.00 4.04e-26 GB AAD52018 "double-stranded RNA-binding zinc finger protein JAZ [Homo sapiens]" 100.00 294 100.00 100.00 3.96e-26 GB AAH07775 "Zinc finger protein 346 [Homo sapiens]" 100.00 294 100.00 100.00 3.96e-26 GB AIC50988 "ZNF346, partial [synthetic construct]" 100.00 294 100.00 100.00 3.96e-26 GB EAW85040 "zinc finger protein 346, isoform CRA_c [Homo sapiens]" 100.00 294 100.00 100.00 3.96e-26 GB EQB78229 "zinc finger protein 346 [Camelus ferus]" 64.00 262 100.00 100.00 2.01e-13 REF NP_001126881 "zinc finger protein 346 [Pongo abelii]" 100.00 310 100.00 100.00 4.79e-26 REF NP_001295142 "zinc finger protein 346 isoform b [Homo sapiens]" 100.00 319 100.00 100.00 6.74e-26 REF NP_001295143 "zinc finger protein 346 isoform c [Homo sapiens]" 100.00 262 100.00 100.00 3.72e-26 REF NP_001295147 "zinc finger protein 346 isoform f [Homo sapiens]" 100.00 196 100.00 100.00 5.42e-27 REF NP_001295150 "zinc finger protein 346 isoform h [Homo sapiens]" 86.00 118 100.00 100.00 7.09e-22 SP Q5R4W8 "RecName: Full=Zinc finger protein 346" 100.00 310 100.00 100.00 4.79e-26 SP Q9UL40 "RecName: Full=Zinc finger protein 346; AltName: Full=Just another zinc finger protein" 100.00 294 100.00 100.00 3.96e-26 stop_ save_ ############# # Ligands # ############# save_ZN _Saveframe_category ligand _Mol_type non-polymer _Name_common "ZN (ZINC ION)" _BMRB_code . _PDB_code ZN _Molecular_mass 65.409 _Mol_charge 2 _Mol_paramagnetic . _Mol_aromatic no _Details ; Information obtained from PDB's Chemical Component Dictionary at http://wwpdb-remediation.rutgers.edu/downloads.html Downloaded on Tue May 31 09:39:42 2011 ; loop_ _Atom_name _PDB_atom_name _Atom_type _Atom_chirality _Atom_charge _Atom_oxidation_number _Atom_unpaired_electrons ZN ZN ZN . 2 . ? stop_ _Mol_thiol_state . _Sequence_homology_query_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $JAZ_ZF4 Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $JAZ_ZF4 'recombinant technology' . Escherichia coli . pET21a stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $JAZ_ZF4 0.4 mM '[U-98% 13C; U-98% 15N]' H2O 93 % 'natural abundance' D2O 7 % 'natural abundance' KCl 50 mM 'natural abundance' Tris 25 mM 'natural abundance' DTT 2 mM 'natural abundance' $ZN 50 uM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ save_NMRView _Saveframe_category software _Name NMRView _Version . loop_ _Vendor _Address _Electronic_address 'Johnson, One Moon Scientific' . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DRX _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_CBCA(CO)NH_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $sample_1 save_ save_3D_HNCA_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCA' _Sample_label $sample_1 save_ save_3D_HNCACB_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ save_3D_HN(CO)CA_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(CO)CA' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details '25 mM Tris, pH 7.4, 50 mM KCl, 2 mM DTT, 50 uM ZnSO4 and 7% D2O' loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 75 . mM pH 7.4 . pH pressure 1 . atm temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.00 . indirect . 'separate tube (no insert) similar to the experimental sample tube' . 0.251449530 DSS H 1 'methyl protons' ppm 0.00 internal direct . 'separate tube (no insert) similar to the experimental sample tube' . 1.000000000 DSS N 15 'methyl protons' ppm 0.00 . indirect . 'separate tube (no insert) similar to the experimental sample tube' . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' '3D CBCA(CO)NH' '3D HNCA' '3D HNCACB' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'JAZ ZF4' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 228 1 PRO CA C 63.43 0.1 1 2 229 2 ALA H H 8.264 0.01 1 3 229 2 ALA CA C 52.511 0.1 1 4 229 2 ALA N N 122.779 0.2 1 5 230 3 VAL H H 7.838 0.01 1 6 230 3 VAL CA C 62.4 0.1 1 7 230 3 VAL N N 118.561 0.2 1 8 231 4 THR H H 7.857 0.01 1 9 231 4 THR CA C 62.160 0.1 1 10 231 4 THR N N 116.029 0.2 1 11 232 5 ASP H H 7.951 0.01 1 12 232 5 ASP CA C 54.084 0.1 1 13 232 5 ASP N N 122.311 0.2 1 14 233 6 PHE H H 7.935 0.01 1 15 233 6 PHE CA C 55.476 0.1 1 16 233 6 PHE N N 120.904 0.2 1 17 235 8 ALA H H 8.237 0.01 1 18 235 8 ALA CA C 52.576 0.1 1 19 235 8 ALA N N 124.373 0.2 1 20 236 9 GLY H H 8.178 0.01 1 21 236 9 GLY CA C 45.109 0.1 1 22 236 9 GLY N N 108.341 0.2 1 23 237 10 LYS H H 7.783 0.01 1 24 237 10 LYS CA C 55.543 0.1 1 25 237 10 LYS N N 120.154 0.2 1 26 238 11 GLY H H 7.807 0.01 1 27 238 11 GLY CA C 44.209 0.1 1 28 238 11 GLY N N 108.154 0.2 1 29 239 12 TYR H H 8.237 0.01 1 30 239 12 TYR CA C 55.209 0.1 1 31 239 12 TYR N N 118.654 0.2 1 32 246 19 ILE H H 7.474 0.01 1 33 246 19 ILE CA C 59.009 0.1 1 34 246 19 ILE N N 117.623 0.2 1 35 247 20 VAL H H 8.493 0.01 1 36 247 20 VAL CA C 62.776 0.1 1 37 247 20 VAL N N 123.061 0.2 1 38 249 22 ASN H H 8.546 0.01 1 39 249 22 ASN CA C 54.743 0.1 1 40 249 22 ASN N N 116.498 0.2 1 41 250 23 SER H H 7.327 0.01 1 42 250 23 SER CA C 61.475 0.1 1 43 250 23 SER N N 110.591 0.2 1 44 251 24 ILE H H 8.688 0.01 1 45 251 24 ILE CA C 62.442 0.1 1 46 251 24 ILE N N 123.623 0.2 1 47 252 25 GLU H H 8.688 0.01 1 48 252 25 GLU CA C 60.432 0.1 1 49 252 25 GLU N N 119.525 0.2 1 50 253 26 GLN H H 7.816 0.01 1 51 253 26 GLN CA C 58.476 0.1 1 52 253 26 GLN N N 119.404 0.2 1 53 254 27 TYR H H 8.32 0.01 1 54 254 27 TYR CA C 62.972 0.1 1 55 254 27 TYR N N 123.436 0.2 1 56 255 28 GLN H H 8.704 0.01 1 57 255 28 GLN CA C 58.542 0.1 1 58 255 28 GLN N N 118.842 0.2 1 59 256 29 ALA H H 7.675 0.01 1 60 256 29 ALA CA C 54.576 0.1 1 61 256 29 ALA N N 121.092 0.2 1 62 259 32 SER H H 8.016 0.01 1 63 259 32 SER CA C 58.746 0.1 1 64 259 32 SER N N 115.185 0.2 1 65 260 33 GLY H H 7.261 0.01 1 66 260 33 GLY CA C 44.443 0.1 1 67 260 33 GLY N N 109.373 0.2 1 68 269 42 LYS H H 8.266 0.01 1 69 269 42 LYS CA C 56.176 0.1 1 70 269 42 LYS N N 121.373 0.2 1 71 270 43 THR H H 7.996 0.01 1 72 270 43 THR CA C 61.742 0.1 1 73 270 43 THR N N 115.842 0.2 1 74 271 44 VAL H H 8.078 0.01 1 75 271 44 VAL CA C 62.242 0.1 1 76 271 44 VAL N N 122.873 0.2 1 77 272 45 ALA H H 8.293 0.01 1 78 272 45 ALA CA C 52.543 0.1 1 79 272 45 ALA N N 127.842 0.2 1 80 273 46 SER H H 8.153 0.01 1 81 273 46 SER CA C 58.448 0.1 1 82 273 46 SER N N 115.118 0.2 1 83 274 47 SER H H 8.205 0.01 1 84 274 47 SER CA C 54.382 0.1 1 85 274 47 SER N N 117.922 0.2 1 86 275 48 LEU H H 8.048 0.01 1 87 275 48 LEU CA C 55.384 0.1 1 88 275 48 LEU N N 123.368 0.2 1 89 276 49 GLY H H 8.245 0.01 1 90 276 49 GLY CA C 45.128 0.1 1 91 276 49 GLY N N 109.936 0.2 1 92 277 50 GLN H H 7.665 0.01 1 93 277 50 GLN CA C 56.947 0.1 1 94 277 50 GLN N N 124.464 0.2 1 stop_ save_