data_18128 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Structural Model for a 40-Residue Beta-Amyloid Fibril with Two-Fold Symmetry, Negative Stagger ; _BMRB_accession_number 18128 _BMRB_flat_file_name bmr18128.str _Entry_type original _Submission_date 2011-12-08 _Accession_date 2011-12-08 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Tycko Robert . . 2 Petkova Aneta . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "13C chemical shifts" 93 "15N chemical shifts" 17 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2012-01-11 update author 'update chemical shifts' 2012-01-05 original author 'original release' stop_ loop_ _Related_BMRB_accession_number _Relationship 18131 '40-residue Beta-Amyloid Fibril with Three-Fold Symmetry, Negative Stagger' 18129 '40-residue Beta-Amyloid Fibril with Three-Fold Symmetry, Positive Stagger' 18127 '40-Residue Beta-Amyloid Fibril with Two-Fold Symmetry' stop_ save_ ############################# # Citation for this entry # ############################# save_citation_1 _Saveframe_category entry_citation _Citation_full . _Citation_title 'Experimental Constraints on Quaternary Structure in Alzheimer's Beta-Amyloid Fibrils' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 16401079 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Petkova Aneta . . 2 Yau Wai-Ming . . 3 Tycko Robert . . stop_ _Journal_abbreviation Biochemistry _Journal_volume 45 _Journal_issue 498 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 498 _Page_last 512 _Year 2006 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name '40-Residue Beta-Amyloid Fibril with Two-Fold Symmetry, Negative Stagger' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label entity_1 $beta-amyloid entity_2 $beta-amyloid entity_3 $beta-amyloid entity_4 $beta-amyloid entity_5 $beta-amyloid entity_6 $beta-amyloid entity_7 $beta-amyloid entity_8 $beta-amyloid entity_9 $beta-amyloid entity_10 $beta-amyloid entity_11 $beta-amyloid entity_12 $beta-amyloid stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_beta-amyloid _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common beta-amyloid _Molecular_mass 3375.924 _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 40 _Mol_residue_sequence ; DAEFRHDSGYEVHHQKLVFF AEDVGSNKGAIIGLMVGGVV ; loop_ _Residue_seq_code _Residue_label 1 ASP 2 ALA 3 GLU 4 PHE 5 ARG 6 HIS 7 ASP 8 SER 9 GLY 10 TYR 11 GLU 12 VAL 13 HIS 14 HIS 15 GLN 16 LYS 17 LEU 18 VAL 19 PHE 20 PHE 21 ALA 22 GLU 23 ASP 24 VAL 25 GLY 26 SER 27 ASN 28 LYS 29 GLY 30 ALA 31 ILE 32 ILE 33 GLY 34 LEU 35 MET 36 VAL 37 GLY 38 GLY 39 VAL 40 VAL stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-11-04 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 11435 Amyloid-beta-(1-40) 100.00 40 100.00 100.00 1.45e-18 BMRB 15775 APP_C99 100.00 122 100.00 100.00 1.17e-18 BMRB 17159 Amyloid_beta-Peptide 100.00 40 100.00 100.00 1.45e-18 BMRB 17186 Abeta 100.00 40 100.00 100.00 1.45e-18 BMRB 17764 Abeta 100.00 40 100.00 100.00 1.45e-18 BMRB 17793 Abeta(1-42) 100.00 42 100.00 100.00 1.25e-18 BMRB 17794 Abeta(1-42) 100.00 42 100.00 100.00 1.25e-18 BMRB 17795 Abeta(1-40) 100.00 40 100.00 100.00 1.45e-18 BMRB 17796 Abeta40 100.00 40 100.00 100.00 1.45e-18 BMRB 18052 Pyroglutamate_Abeta 92.50 38 100.00 100.00 2.67e-16 BMRB 18127 beta-amyloid 100.00 40 100.00 100.00 1.45e-18 BMRB 18129 beta-amyloid 100.00 40 100.00 100.00 1.45e-18 BMRB 18131 beta-amyloid 100.00 40 100.00 100.00 1.45e-18 BMRB 19009 beta-amyloid_peptide 100.00 40 100.00 100.00 1.45e-18 BMRB 19309 amyloid_peptide 100.00 40 100.00 100.00 1.45e-18 BMRB 19393 Abeta 100.00 39 97.50 97.50 5.22e-16 BMRB 25218 amyloid_peptide 100.00 42 100.00 100.00 1.25e-18 BMRB 25289 amyloid_beta 100.00 39 97.50 97.50 5.22e-16 BMRB 25429 entity 100.00 42 100.00 100.00 1.25e-18 BMRB 26508 amyloid_B 100.00 40 100.00 100.00 1.45e-18 BMRB 26516 amyloid_B 100.00 40 100.00 100.00 1.45e-18 PDB 1AMB "Solution Structure Of Residues 1-28 Of The Amyloid Beta- Peptide" 70.00 28 100.00 100.00 2.06e-10 PDB 1AMC "Solution Structure Of Residues 1-28 Of The Amyloid Beta- Peptide" 70.00 28 100.00 100.00 2.06e-10 PDB 1AML "The Alzheimer`s Disease Amyloid A4 Peptide (Residues 1-40)" 100.00 40 100.00 100.00 1.45e-18 PDB 1BA4 "The Solution Structure Of Amyloid Beta-Peptide (1-40) In A Water-Micelle Environment. Is The Membrane-Spanning Domain Where We " 100.00 40 100.00 100.00 1.45e-18 PDB 1BA6 "Solution Structure Of The Methionine-Oxidized Amyloid Beta- Peptide (1-40). Does Oxidation Affect Conformational Switching? Nmr" 100.00 40 97.50 97.50 1.63e-17 PDB 1HZ3 "Alzheimer's Disease Amyloid-Beta Peptide (Residues 10-35)" 65.00 26 100.00 100.00 2.01e-08 PDB 1IYT "Solution Structure Of The Alzheimer's Disease Amyloid Beta- Peptide (1-42)" 100.00 42 100.00 100.00 1.25e-18 PDB 1Z0Q "Aqueous Solution Structure Of The Alzheimer's Disease Abeta Peptide (1-42)" 100.00 42 100.00 100.00 1.25e-18 PDB 2BEG "3d Structure Of Alzheimer's Abeta(1-42) Fibrils" 100.00 42 100.00 100.00 1.25e-18 PDB 2G47 "Crystal Structure Of Human Insulin-Degrading Enzyme In Complex With Amyloid-Beta (1-40)" 100.00 40 100.00 100.00 1.45e-18 PDB 2LFM "A Partially Folded Structure Of Amyloid-Beta(1 40) In An Aqueous Environment" 100.00 40 100.00 100.00 1.45e-18 PDB 2LMN "Structural Model For A 40-Residue Beta-Amyloid Fibril With Two-Fold Symmetry, Positive Stagger" 100.00 40 100.00 100.00 1.45e-18 PDB 2LMO "Structural Model For A 40-Residue Beta-Amyloid Fibril With Two-Fold Symmetry, Negative Stagger" 100.00 40 100.00 100.00 1.45e-18 PDB 2LMP "Structural Model For A 40-Residue Beta-Amyloid Fibril With Three-Fold Symmetry, Positive Stagger" 100.00 40 100.00 100.00 1.45e-18 PDB 2LMQ "Structural Model For A 40-Residue Beta-Amyloid Fibril With Three-Fold Symmetry, Negative Stagger" 100.00 40 100.00 100.00 1.45e-18 PDB 2LNQ "40-residue D23n Beta Amyloid Fibril" 100.00 40 97.50 100.00 4.98e-18 PDB 2LP1 "The Solution Nmr Structure Of The Transmembrane C-Terminal Domain Of The Amyloid Precursor Protein (C99)" 100.00 122 100.00 100.00 1.17e-18 PDB 2M4J "40-residue Beta-amyloid Fibril Derived From Alzheimer's Disease Brain" 100.00 40 100.00 100.00 1.45e-18 PDB 2M9R "3d Nmr Structure Of A Complex Between The Amyloid Beta Peptide (1-40) And The Polyphenol Epsilon-viniferin Glucoside" 100.00 40 100.00 100.00 1.45e-18 PDB 2M9S "3d Nmr Structure Of A Complex Between The Amyloid Beta Peptide (1-40) And The Polyphenol Epsilon-viniferin Glucoside" 100.00 40 100.00 100.00 1.45e-18 PDB 2MVX "Atomic-resolution 3d Structure Of Amyloid-beta Fibrils: The Osaka Mutation" 100.00 39 97.50 97.50 5.22e-16 PDB 2MXU "42-residue Beta Amyloid Fibril" 100.00 42 100.00 100.00 1.25e-18 PDB 2OTK "Structure Of Alzheimer Ab Peptide In Complex With An Engineered Binding Protein" 100.00 40 100.00 100.00 1.45e-18 PDB 2WK3 "Crystal Structure Of Human Insulin-Degrading Enzyme In Complex With Amyloid-Beta (1-42)" 100.00 42 100.00 100.00 1.25e-18 PDB 3BAE "Crystal Structure Of Fab Wo2 Bound To The N Terminal Domain Of Amyloid Beta Peptide (1-28)" 70.00 28 100.00 100.00 2.06e-10 PDB 3IFN "X-ray Structure Of Amyloid Beta Peptide:antibody (abeta1-40:12a11) Complex" 100.00 40 100.00 100.00 1.45e-18 PDB 4HIX "Crystal Structure Of A Humanised 3d6 Fab Bound To Amyloid Beta Peptide" 70.00 28 100.00 100.00 2.06e-10 PDB 4M1C "Crystal Structure Analysis Of Fab-bound Human Insulin Degrading Enzyme (ide) In Complex With Amyloid-beta (1-40)" 100.00 40 100.00 100.00 1.45e-18 PDB 4MVI "Crystal Structure Of An Engineered Lipocalin (anticalin Us7) In Complex With The Alzheimer Amyloid Peptide Abeta(1-40)" 100.00 40 100.00 100.00 1.45e-18 PDB 4MVL "Crystal Structure Of An Engineered Lipocalin (anticalin H1ga) In Complex With The Alzheimer Amyloid Peptide Abeta1-40" 100.00 40 100.00 100.00 1.45e-18 PDB 4NGE "Crystal Structure Of Human Presequence Protease In Complex With Amyloid-beta (1-40)" 100.00 40 100.00 100.00 1.45e-18 PDB 4ONG "Fab Fragment Of 3d6 In Complex With Amyloid Beta 1-40" 100.00 40 100.00 100.00 1.45e-18 PDB 5AEF "Electron Cryo-microscopy Of An Abeta(1-42)amyloid Fibril" 65.00 28 100.00 100.00 3.64e-07 DBJ BAA22264 "amyloid precursor protein [Homo sapiens]" 100.00 770 100.00 100.00 1.24e-18 DBJ BAA84580 "amyloid precursor protein [Sus scrofa]" 100.00 770 100.00 100.00 1.24e-18 DBJ BAB71958 "amyloid precursor protein [Homo sapiens]" 100.00 52 97.50 100.00 1.70e-18 DBJ BAD51938 "amyloid beta A4 precursor protein [Macaca fascicularis]" 100.00 696 100.00 100.00 1.11e-18 DBJ BAE01907 "unnamed protein product [Macaca fascicularis]" 100.00 751 100.00 100.00 1.39e-18 EMBL CAA30050 "amyloid A4 protein [Homo sapiens]" 100.00 751 100.00 100.00 1.21e-18 EMBL CAA31830 "A4 amyloid protein precursor [Homo sapiens]" 100.00 695 100.00 100.00 1.27e-18 EMBL CAA39589 "amyloid precursor protein [Bos taurus]" 100.00 59 100.00 100.00 3.47e-19 EMBL CAA39590 "amyloid precursor protein [Canis lupus familiaris]" 100.00 58 100.00 100.00 3.58e-19 EMBL CAA39591 "amyloid precursor protein [Cavia sp.]" 100.00 58 100.00 100.00 3.58e-19 GB AAA35540 "amyloid protein, partial [Homo sapiens]" 95.00 97 100.00 100.00 2.29e-17 GB AAA36829 "amyloid b-protein precursor [Macaca fascicularis]" 100.00 695 100.00 100.00 1.27e-18 GB AAA51564 "amyloid beta protein, partial [Homo sapiens]" 75.00 30 100.00 100.00 1.06e-11 GB AAA51722 "amyloid beta-protein precursor, partial [Homo sapiens]" 100.00 412 100.00 100.00 8.90e-19 GB AAA51726 "beta-amyloid A4, partial [Homo sapiens]" 100.00 264 100.00 100.00 1.69e-18 PIR A60045 "Alzheimer's disease amyloid beta/A4 protein precursor - dog (fragment)" 100.00 57 100.00 100.00 3.90e-19 PIR D60045 "Alzheimer's disease amyloid beta/A4 protein precursor - bovine (fragment)" 100.00 57 100.00 100.00 3.90e-19 PIR E60045 "Alzheimer's disease amyloid beta/A4 protein precursor - sheep (fragment)" 100.00 57 100.00 100.00 3.90e-19 PIR G60045 "Alzheimer's disease amyloid beta/A4 protein precursor - guinea pig (fragment)" 100.00 57 100.00 100.00 3.90e-19 PIR PQ0438 "Alzheimer's disease amyloid A4 protein precursor - rabbit (fragment)" 100.00 82 100.00 100.00 3.95e-19 PRF 1303338A "amyloid A4 protein precursor" 100.00 695 100.00 100.00 1.27e-18 PRF 1403400A "amyloid protein A4" 100.00 751 100.00 100.00 1.21e-18 PRF 1405204A "amyloid protein" 100.00 42 100.00 100.00 1.25e-18 PRF 1507304A "beta amyloid peptide precursor" 100.00 412 100.00 100.00 9.17e-19 PRF 1507304B "beta amyloid peptide precursor" 100.00 574 100.00 100.00 3.96e-18 REF NP_000475 "amyloid beta A4 protein isoform a precursor [Homo sapiens]" 100.00 770 100.00 100.00 1.24e-18 REF NP_001006601 "amyloid beta A4 protein isoform APP-770 precursor [Canis lupus familiaris]" 100.00 770 100.00 100.00 1.24e-18 REF NP_001013036 "amyloid beta A4 protein precursor [Pan troglodytes]" 100.00 770 100.00 100.00 1.24e-18 REF NP_001070264 "amyloid beta A4 protein precursor [Bos taurus]" 100.00 695 100.00 100.00 1.27e-18 REF NP_001127014 "amyloid beta A4 protein precursor [Pongo abelii]" 100.00 695 100.00 100.00 1.60e-18 SP P05067 "RecName: Full=Amyloid beta A4 protein; AltName: Full=ABPP; AltName: Full=APPI; Short=APP; AltName: Full=Alzheimer disease amylo" 100.00 770 100.00 100.00 1.24e-18 SP P53601 "RecName: Full=Amyloid beta A4 protein; AltName: Full=ABPP; Short=APP; AltName: Full=Alzheimer disease amyloid A4 protein homolo" 100.00 770 100.00 100.00 1.24e-18 SP P79307 "RecName: Full=Amyloid beta A4 protein; AltName: Full=ABPP; Short=APP; AltName: Full=Alzheimer disease amyloid A4 protein homolo" 100.00 770 100.00 100.00 1.24e-18 SP P86906 "RecName: Full=Amyloid beta A4 protein; AltName: Full=ABPP; Short=APP; AltName: Full=Alzheimer disease amyloid A4 protein homolo" 100.00 40 97.50 100.00 5.37e-18 SP Q28053 "RecName: Full=Amyloid beta A4 protein; AltName: Full=ABPP; Short=APP; AltName: Full=Alzheimer disease amyloid A4 protein homolo" 100.00 59 100.00 100.00 3.47e-19 TPG DAA33655 "TPA: amyloid beta A4 protein [Bos taurus]" 100.00 695 100.00 100.00 1.27e-18 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $beta-amyloid human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $beta-amyloid 'chemical synthesis' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type 'amyloid fibril' _Details 'grown at room temperature with gentle agitation, 1 mg/ml peptide, pH 7.4' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $beta-amyloid 1 mg/mL 'selective 13C and 15N' 'phosphate buffer' 10 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_X-PLOR_NIH _Saveframe_category software _Name X-PLOR_NIH _Version . loop_ _Vendor _Address _Electronic_address 'Schwieters, Kuszewski, Tjandra and Clore' . . stop_ loop_ _Task 'structure solution' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model Infinity _Field_strength 400 _Details . save_ save_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model Infinity _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_fpRFDR_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D fpRFDR' _Sample_label $sample_1 save_ save_2D_RAD_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D RAD' _Sample_label $sample_1 save_ save_2D_TEDOR_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D TEDOR' _Sample_label $sample_1 save_ save_fsREDOR_4 _Saveframe_category NMR_applied_experiment _Experiment_name fsREDOR _Sample_label $sample_1 save_ save_15N-BARE_5 _Saveframe_category NMR_applied_experiment _Experiment_name 15N-BARE _Sample_label $sample_1 save_ save_13C-BARE_6 _Saveframe_category NMR_applied_experiment _Experiment_name 13C-BARE _Sample_label $sample_1 save_ save_PITHIRDS-CT_7 _Saveframe_category NMR_applied_experiment _Experiment_name PITHIRDS-CT _Sample_label $sample_1 save_ save_2D_NCACX_8 _Saveframe_category NMR_applied_experiment _Experiment_name '2D NCACX' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units temperature 300 . K pH 7.4 . pH pressure 1 . atm 'ionic strength' 10 . mM stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details 'DSS reference for 13C shifts. Liquid NH3 reference for 15N shifts.' loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl carbons' ppm 0 external direct . . . 1.0 'liquid anhydrous ammonia' N 15 nitrogen ppm 0 external direct . . . 1.0 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D fpRFDR' '2D NCACX' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name entity_1 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 2 2 ALA C C 175.4 0.3 1 2 2 2 ALA CA C 51.1 0.3 1 3 2 2 ALA CB C 22.5 0.3 1 4 7 7 ASP C C 174 0.3 1 5 7 7 ASP CA C 53.6 0.3 1 6 7 7 ASP CB C 42.4 0.3 1 7 8 8 SER C C 172.7 0.3 1 8 8 8 SER CA C 58.1 0.3 1 9 8 8 SER CB C 65.3 0.3 1 10 9 9 GLY C C 172.1 0.3 1 11 9 9 GLY CA C 44.7 0.3 1 12 10 10 TYR C C 175.0 0.3 1 13 10 10 TYR CA C 57.3 0.3 1 14 10 10 TYR CB C 42.1 0.3 1 15 12 12 VAL C C 174.6 0.3 1 16 12 12 VAL CA C 60.4 0.3 1 17 12 12 VAL CB C 35.3 0.3 1 18 13 13 HIS C C 174.2 0.3 1 19 13 13 HIS CA C 54.2 0.3 1 20 13 13 HIS CB C 34.8 0.3 1 21 14 14 HIS C C 174.2 0.3 1 22 14 14 HIS CA C 55.4 0.3 1 23 14 14 HIS CB C 33.1 0.3 1 24 15 15 GLN C C 173.6 0.3 1 25 15 15 GLN CA C 55.0 0.3 1 26 15 15 GLN CB C 33.5 0.3 1 27 15 15 GLN N N 123.8 0.5 1 28 16 16 LYS C C 174.1 0.3 1 29 16 16 LYS CA C 54.7 0.3 1 30 16 16 LYS CB C 36.0 0.3 1 31 16 16 LYS N N 126.4 0.5 1 32 17 17 LEU C C 174.6 0.3 1 33 17 17 LEU CA C 54.5 0.3 1 34 17 17 LEU CB C 46.4 0.3 1 35 18 18 VAL C C 172.8 0.3 1 36 18 18 VAL CA C 60.9 0.3 1 37 18 18 VAL CB C 35.6 0.3 1 38 19 19 PHE C C 171.9 0.3 1 39 19 19 PHE CA C 56.5 0.3 1 40 19 19 PHE CB C 42.6 0.3 1 41 20 20 PHE C C 172.2 0.3 1 42 20 20 PHE CA C 56.0 0.3 1 43 20 20 PHE CB C 42.9 0.3 1 44 21 21 ALA C C 175 0.3 1 45 21 21 ALA CA C 50.1 0.3 1 46 21 21 ALA CB C 22.7 0.3 1 47 22 22 GLU C C 174.9 0.3 1 48 22 22 GLU CA C 53.9 0.3 1 49 22 22 GLU CB C 34.1 0.3 1 50 23 23 ASP C C 174.2 0.3 1 51 23 23 ASP CA C 56.8 0.3 1 52 23 23 ASP CB C 39.2 0.3 1 53 23 23 ASP N N 126.9 0.5 1 54 24 24 VAL C C 174.5 0.3 1 55 24 24 VAL CA C 60.3 0.3 1 56 24 24 VAL CB C 32.9 0.3 1 57 24 24 VAL N N 121.2 0.5 1 58 25 25 GLY C C 171.3 0.3 1 59 25 25 GLY CA C 47.0 0.3 1 60 25 25 GLY N N 118.0 0.5 1 61 26 26 SER C C 174.1 0.3 1 62 26 26 SER CA C 56.4 0.3 1 63 26 26 SER CB C 65.7 0.3 1 64 26 26 SER N N 112.6 0.5 1 65 27 27 ASN C C 174.3 0.3 1 66 27 27 ASN CA C 53.3 0.3 1 67 27 27 ASN CB C 40.4 0.3 1 68 27 27 ASN N N 124.0 0.5 1 69 28 28 LYS C C 173.6 0.3 1 70 28 28 LYS CA C 55.5 0.3 1 71 28 28 LYS CB C 35.0 0.3 1 72 28 28 LYS N N 124.0 0.5 1 73 29 29 GLY C C 171.4 0.3 1 74 29 29 GLY CA C 44.0 0.3 1 75 29 29 GLY N N 107.5 0.5 1 76 30 30 ALA C C 174.8 0.3 1 77 30 30 ALA CA C 49.9 0.3 1 78 30 30 ALA CB C 22.7 0.3 1 79 30 30 ALA N N 127.8 0.5 1 80 31 31 ILE C C 174.1 0.3 1 81 31 31 ILE CA C 60.8 0.3 1 82 31 31 ILE CB C 40.2 0.3 1 83 31 31 ILE N N 117.9 0.5 1 84 32 32 ILE C C 174.8 0.3 1 85 32 32 ILE CA C 58.2 0.3 1 86 32 32 ILE CB C 42.4 0.3 1 87 33 33 GLY C C 171.6 0.3 1 88 33 33 GLY CA C 48.8 0.3 1 89 33 33 GLY N N 110.8 0.5 1 90 34 34 LEU C C 173.5 0.3 1 91 34 34 LEU CA C 53.8 0.3 1 92 34 34 LEU CB C 45.9 0.3 1 93 35 35 MET C C 173.3 0.3 1 94 35 35 MET CA C 54.4 0.3 1 95 35 35 MET CB C 37.1 0.3 1 96 35 35 MET N N 122.1 0.5 1 97 36 36 VAL C C 175.2 0.3 1 98 36 36 VAL CA C 59.7 0.3 1 99 36 36 VAL CB C 34.9 0.3 1 100 36 36 VAL N N 124.3 0.5 1 101 37 37 GLY C C 171.0 0.3 1 102 37 37 GLY CA C 44.9 0.3 1 103 37 37 GLY N N 116.5 0.5 1 104 38 38 GLY C C 170.4 0.3 1 105 38 38 GLY CA C 44.8 0.3 1 106 38 38 GLY N N 104.2 0.5 1 107 39 39 VAL C C 173.2 0.3 1 108 39 39 VAL CA C 61.1 0.3 1 109 39 39 VAL CB C 35.2 0.3 1 110 39 39 VAL N N 114.7 0.5 1 stop_ save_