data_18610 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; 1H, 13C and 15N assignments of Ubiquitin for both folded and denatured states at 258K and 2500 bar ; _BMRB_accession_number 18610 _BMRB_flat_file_name bmr18610.str _Entry_type original _Submission_date 2012-07-23 _Accession_date 2012-07-23 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Vajpai Navratna . . 2 Nisius Lydia . . 3 Wiktor Maciej . . 4 Grzesiek Stephan . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 2 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 115 "13C chemical shifts" 287 "15N chemical shifts" 115 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2013-02-12 original author . stop_ loop_ _Related_BMRB_accession_number _Relationship 18611 'Pressure & methanol-induced A-state of Ubiquitin' stop_ _Original_release_date 2013-02-12 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'High-pressure NMR reveals close similarity between cold and alcohol protein denaturation in ubiquitin.' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 23284170 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Vajpai Navratna . . 2 Nisius Lydia . . 3 Wiktor Maciej . . 4 Grzesiek Stephan . . stop_ _Journal_abbreviation 'Proc. Natl. Acad. Sci. U.S.A.' _Journal_name_full 'Proceedings of the National Academy of Sciences of the United States of America' _Journal_volume 110 _Journal_issue 5 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first . _Page_last . _Year 2013 _Details . loop_ _Keyword 'cold denaturation' 'heteronuclear NMR' 'High pressure' 'protein folding' thermodynamics stop_ save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name Ubiquitin _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label Ubiquitin_folded $Ubiquitin Ubiquitin_denatured $Ubiquitin stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_Ubiquitin _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common Ubiquitin _Molecular_mass . _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 76 _Mol_residue_sequence ; MQIFVKTLTGKTITLEVEPS DTIENVKAKIQDKEGIPPDQ QRLIFAGKQLEDGRTLSDYN IQKESTLHLVLRLRGG ; loop_ _Residue_seq_code _Residue_label 1 MET 2 GLN 3 ILE 4 PHE 5 VAL 6 LYS 7 THR 8 LEU 9 THR 10 GLY 11 LYS 12 THR 13 ILE 14 THR 15 LEU 16 GLU 17 VAL 18 GLU 19 PRO 20 SER 21 ASP 22 THR 23 ILE 24 GLU 25 ASN 26 VAL 27 LYS 28 ALA 29 LYS 30 ILE 31 GLN 32 ASP 33 LYS 34 GLU 35 GLY 36 ILE 37 PRO 38 PRO 39 ASP 40 GLN 41 GLN 42 ARG 43 LEU 44 ILE 45 PHE 46 ALA 47 GLY 48 LYS 49 GLN 50 LEU 51 GLU 52 ASP 53 GLY 54 ARG 55 THR 56 LEU 57 SER 58 ASP 59 TYR 60 ASN 61 ILE 62 GLN 63 LYS 64 GLU 65 SER 66 THR 67 LEU 68 HIS 69 LEU 70 VAL 71 LEU 72 ARG 73 LEU 74 ARG 75 GLY 76 GLY stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-11-25 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 11505 entity 100.00 76 98.68 98.68 3.54e-45 BMRB 11547 ubiquitin 100.00 76 98.68 98.68 3.54e-45 BMRB 15047 denatured_ubiquitin 100.00 76 100.00 100.00 6.54e-46 BMRB 15410 Ubi 100.00 76 100.00 100.00 6.54e-46 BMRB 15689 UBB 98.68 103 98.67 100.00 6.21e-44 BMRB 15907 Ubiquitin 100.00 76 100.00 100.00 6.54e-46 BMRB 16228 ubiquitin 100.00 76 97.37 98.68 1.73e-44 BMRB 16582 Ubiquitin 100.00 76 100.00 100.00 6.54e-46 BMRB 16626 Ubiquitin 100.00 76 100.00 100.00 6.54e-46 BMRB 16895 UBB+1 98.68 103 98.67 100.00 6.21e-44 BMRB 17181 ubiquitin 100.00 76 100.00 100.00 6.54e-46 BMRB 17439 ubiquitin 100.00 76 100.00 100.00 6.54e-46 BMRB 17769 Ubiquitin 100.00 76 100.00 100.00 6.54e-46 BMRB 17919 entity 100.00 76 100.00 100.00 6.54e-46 BMRB 18582 ubiquitin 100.00 76 100.00 100.00 6.54e-46 BMRB 18583 ubiquitin 100.00 76 100.00 100.00 6.54e-46 BMRB 18584 ubiquitin 100.00 76 100.00 100.00 6.54e-46 BMRB 18611 Ubiquitin_A_state 100.00 76 100.00 100.00 6.54e-46 BMRB 18737 UBIQUITIN 100.00 76 100.00 100.00 6.54e-46 BMRB 19406 entity 100.00 152 100.00 100.00 6.43e-45 BMRB 19412 entity 100.00 152 100.00 100.00 6.43e-45 BMRB 25070 Ubiquitin 100.00 79 100.00 100.00 6.66e-46 BMRB 25123 Ubiquitin 94.74 72 100.00 100.00 8.52e-43 BMRB 25601 entity_1 100.00 76 97.37 97.37 5.31e-44 BMRB 26604 Ubiquitin_(microcrystalline) 100.00 76 100.00 100.00 6.54e-46 BMRB 4245 ubiquitin 100.00 76 100.00 100.00 6.54e-46 BMRB 4375 Ubiquitin 100.00 76 100.00 100.00 6.54e-46 PDB 1AAR "Structure Of A Diubiquitin Conjugate And A Model For Interaction With Ubiquitin Conjugating Enzyme (E2)" 100.00 76 100.00 100.00 6.54e-46 PDB 1CMX "Structural Basis For The Specificity Of Ubiquitin C- Terminal Hydrolases" 98.68 76 100.00 100.00 4.40e-45 PDB 1D3Z "Ubiquitin Nmr Structure" 100.00 76 100.00 100.00 6.54e-46 PDB 1F9J "Structure Of A New Crystal Form Of Tetraubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 1FXT "Structure Of A Conjugating Enzyme-Ubiquitin Thiolester Complex" 100.00 76 100.00 100.00 6.54e-46 PDB 1G6J "Structure Of Recombinant Human Ubiquitin In Aot Reverse Micelles" 98.68 76 100.00 100.00 7.58e-45 PDB 1GJZ "Solution Structure Of A Dimeric N-Terminal Fragment Of Human Ubiquitin" 67.11 53 100.00 100.00 3.14e-26 PDB 1NBF "Crystal Structure Of A Ubp-Family Deubiquitinating Enzyme In Isolation And In Complex With Ubiquitin Aldehyde" 98.68 76 100.00 100.00 4.40e-45 PDB 1OGW "Synthetic Ubiquitin With Fluoro-Leu At 50 And 67" 100.00 76 97.37 97.37 2.65e-44 PDB 1P3Q "Mechanism Of Ubiquitin Recognition By The Cue Domain Of Vps9" 100.00 76 100.00 100.00 6.54e-46 PDB 1Q5W "Ubiquitin Recognition By Npl4 Zinc-Fingers" 100.00 76 100.00 100.00 6.54e-46 PDB 1S1Q "Tsg101(Uev) Domain In Complex With Ubiquitin" 98.68 76 100.00 100.00 7.58e-45 PDB 1TBE "Structure Of Tetraubiquitin Shows How Multiubiquitin Chains Can Be Formed" 100.00 76 100.00 100.00 6.54e-46 PDB 1UBI "Synthetic Structural And Biological Studies Of The Ubiquitin System. Part 1" 100.00 76 100.00 100.00 6.54e-46 PDB 1UBQ "Structure Of Ubiquitin Refined At 1.8 Angstroms Resolution" 100.00 76 100.00 100.00 6.54e-46 PDB 1UZX "A Complex Of The Vps23 Uev With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 1V80 "Solution Structures Of Ubiquitin At 30 Bar And 3 Kbar" 100.00 76 100.00 100.00 6.54e-46 PDB 1V81 "Solution Structures Of Ubiquitin At 30 Bar And 3 Kbar" 100.00 76 100.00 100.00 6.54e-46 PDB 1VX7 "Cryo-em Structure Of The Plasmodium Falciparum 80s Ribosome Bound To The Anti-protozoan Drug Emetine, Large Subunit (protein On" 100.00 128 98.68 100.00 1.38e-45 PDB 1WR6 "Crystal Structure Of Gga3 Gat Domain In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 1WRD "Crystal Structure Of Tom1 Gat Domain In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 1XD3 "Crystal Structure Of Uchl3-Ubvme Complex" 98.68 75 100.00 100.00 4.28e-45 PDB 1XQQ "Simultaneous Determination Of Protein Structure And Dynamics" 100.00 76 100.00 100.00 6.54e-46 PDB 1YD8 "Complex Of Human Gga3 Gat Domain And Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 1YIW "X-Ray Crystal Structure Of A Chemically Synthesized Ubiquitin" 100.00 76 98.68 100.00 2.84e-45 PDB 1YJ1 "X-Ray Crystal Structure Of A Chemically Synthesized [d-Gln35]ubiquitin" 100.00 76 97.37 98.68 3.36e-44 PDB 1YX5 "Solution Structure Of S5a Uim-1UBIQUITIN COMPLEX" 100.00 98 100.00 100.00 2.31e-46 PDB 1YX6 "Solution Structure Of S5a Uim-2UBIQUITIN COMPLEX" 100.00 98 100.00 100.00 2.31e-46 PDB 1ZGU "Solution Structure Of The Human Mms2-Ubiquitin Complex" 100.00 76 98.68 100.00 1.56e-45 PDB 2AYO "Structure Of Usp14 Bound To Ubquitin Aldehyde" 98.68 76 100.00 100.00 4.40e-45 PDB 2BGF "Nmr Structure Of Lys48-Linked Di-Ubiquitin Using Chemical Shift Perturbation Data Together With Rdcs And 15n- Relaxation Data" 100.00 76 100.00 100.00 6.54e-46 PDB 2C7M "Human Rabex-5 Residues 1-74 In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2C7N "Human Rabex-5 Residues 1-74 In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2D3G "Double Sided Ubiquitin Binding Of Hrs-Uim" 100.00 76 100.00 100.00 6.54e-46 PDB 2DEN "Solution Structure Of The Ubiquitin-Associated Domain Of Human Bmsc-Ubp And Its Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2DX5 "The Complex Structure Between The Mouse Eap45-Glue Domain And Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2FCM "X-Ray Crystal Structure Of A Chemically Synthesized [d-Gln35]ubiquitin With A Cubic Space Group" 100.00 76 97.37 98.68 3.36e-44 PDB 2FCN "X-Ray Crystal Structure Of A Chemically Synthesized [d-Val35]ubiquitin With A Cubic Space Group" 100.00 76 97.37 98.68 3.36e-44 PDB 2FCQ "X-Ray Crystal Structure Of A Chemically Synthesized Ubiquitin With A Cubic Space Group" 100.00 76 98.68 100.00 2.84e-45 PDB 2FCS "X-Ray Crystal Structure Of A Chemically Synthesized [l-Gln35]ubiquitin With A Cubic Space Group" 100.00 76 97.37 98.68 3.83e-44 PDB 2FID "Crystal Structure Of A Bovine Rabex-5 Fragment Complexed With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2FIF "Crystal Structure Of A Bovine Rabex-5 Fragment Complexed With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2FUH "Solution Structure Of The Ubch5cUB NON-Covalent Complex" 100.00 76 100.00 100.00 6.54e-46 PDB 2G45 "Co-Crystal Structure Of Znf Ubp Domain From The Deubiquitinating Enzyme Isopeptidase T (Isot) In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2GMI Mms2UBC13~UBIQUITIN 100.00 76 100.00 100.00 6.54e-46 PDB 2HD5 "Usp2 In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2HTH "Structural Basis For Ubiquitin Recognition By The Human Eap45ESCRT-Ii Glue Domain" 100.00 76 100.00 100.00 6.54e-46 PDB 2IBI "Covalent Ubiquitin-Usp2 Complex" 98.68 75 100.00 100.00 4.28e-45 PDB 2J7Q "Crystal Structure Of The Ubiquitin-Specific Protease Encoded By Murine Cytomegalovirus Tegument Protein M48 In Complex With A U" 98.68 75 100.00 100.00 4.28e-45 PDB 2JF5 "Crystal Structure Of Lys63-Linked Di-Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2JRI "Solution Structure Of The Josephin Domain Of Ataxin-3 In Complex With Ubiquitin Molecule." 100.00 76 100.00 100.00 6.54e-46 PDB 2JY6 "Solution Structure Of The Complex Of Ubiquitin And Ubiquilin 1 Uba Domain" 100.00 76 100.00 100.00 6.54e-46 PDB 2JZZ "Solid-State Nmr Structure Of Microcrystalline Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2K25 "Automated Nmr Structure Of The Ubb By Fapsy" 98.68 103 98.67 100.00 6.21e-44 PDB 2K39 "Recognition Dynamics Up To Microseconds Revealed From Rdc Derived Ubiquitin Ensemble In Solution" 100.00 76 100.00 100.00 6.54e-46 PDB 2K6D "Cin85 Sh3-C Domain In Complex With Ubiquitin" 98.68 76 100.00 100.00 3.99e-45 PDB 2K8B "Solution Structure Of Plaa Family Ubiquitin Binding Domain (Pfuc) Cis Isomer In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2K8C "Solution Structure Of Plaa Family Ubiquitin Binding Domain (Pfuc) Trans Isomer In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2KDE "Nmr Structure Of Major S5a (196-306):k48 Linked Diubiquitin Species" 100.00 76 100.00 100.00 6.54e-46 PDB 2KDF "Nmr Structure Of Minor S5a (196-306):k48 Linked Diubiquitin Species" 100.00 76 100.00 100.00 6.54e-46 PDB 2KHW "Solution Structure Of The Human Polymerase Iota Ubm2- Ubiquitin Complex" 100.00 79 100.00 100.00 6.66e-46 PDB 2KJH "Nmr Based Structural Model Of The Ubch8-Ubiquitin Complex" 98.68 76 100.00 100.00 3.99e-45 PDB 2KLG "Pere Nmr Structure Of Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2KN5 "A Correspondence Between Solution-State Dynamics Of An Individual Protein And The Sequence And Conformational Diversity Of Its " 100.00 76 100.00 100.00 6.54e-46 PDB 2KOX "Nmr Residual Dipolar Couplings Identify Long Range Correlated Motions In The Backbone Of The Protein Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2KTF "Solution Nmr Structure Of Human Polymerase Iota Ubm2 In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2KWU "Solution Structure Of Ubm2 Of Murine Polymerase Iota In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2KWV "Solution Structure Of Ubm1 Of Murine Polymerase Iota In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2KX0 "The Solution Structure Of Ubb+1, Frameshift Mutant Of Ubiquitin B" 98.68 103 98.67 100.00 6.21e-44 PDB 2L0F "Solution Nmr Structure Of Human Polymerase Iota Ubm2 (P692a Mutant) In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2L0T "Solution Structure Of The Complex Of Ubiquitin And The Vhs Domain Of Stam2" 100.00 76 100.00 100.00 6.54e-46 PDB 2L3Z "Proton-Detected 4d Dream Solid-State Nmr Structure Of Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2LD9 "Backbone Structure Of Ubiquitin Determined Using Backbone Amide Noes And Backbone N-H And N-C Rdcs" 100.00 77 100.00 100.00 7.92e-46 PDB 2LJ5 "Description Of The Structural Fluctuations Of Proteins From Structure- Based Calculations Of Residual Dipolar Couplings" 100.00 76 100.00 100.00 6.54e-46 PDB 2LVO "Structure Of The Gp78cue Domain Bound To Monubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2LVP "Gp78cue Domain Bound To The Distal Ubiquitin Of K48-Linked Diubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2LVQ "Gp78cue Domain Bound To The Proximal Ubiquitin Of K48-Linked Diubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2LZ6 "Distinct Ubiquitin Binding Modes Exhibited By Sh3 Domains: Molecular Determinants And Functional Implications" 100.00 76 100.00 100.00 6.54e-46 PDB 2MBB "Solution Structure Of The Human Polymerase Iota Ubm1-ubiquitin Complex" 100.00 78 100.00 100.00 5.38e-46 PDB 2MBH "Nmr Structure Of Eklf(22-40)/ubiquitin Complex" 100.00 76 100.00 100.00 6.54e-46 PDB 2MBO "K11-linked Diubiquitin Average Solution Structure At Ph 6.8, 0 Mm Nacl" 100.00 76 100.00 100.00 6.54e-46 PDB 2MBQ "K11-linked Diubiquitin Average Solution Structure At Ph 6.8, 150 Mm Nacl" 100.00 76 100.00 100.00 6.54e-46 PDB 2MCN "Distinct Ubiquitin Binding Modes Exhibited By Sh3 Domains: Molecular Determinants And Functional Implications" 100.00 76 100.00 100.00 6.54e-46 PDB 2MJ5 "Structure Of The Uba Domain Of Human Nbr1 In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2MJB "Solution Nmr Structure Of Ubiquitin Refined Against Dipolar Couplings In 4 Media" 100.00 76 100.00 100.00 6.54e-46 PDB 2MOR "A Tensor-free Method For The Structural And Dynamical Refinement Of Proteins Using Residual Dipolar Couplings" 100.00 76 100.00 100.00 6.54e-46 PDB 2MRE "Nmr Structure Of The Rad18-ubz/ubiquitin Complex" 100.00 79 100.00 100.00 6.66e-46 PDB 2MRO "Structure Of The Complex Of Ubiquitin And The Uba Domain From Dna- Damage-inducible 1 Protein (ddi1)" 100.00 76 100.00 100.00 6.54e-46 PDB 2MSG "Solid-state Nmr Structure Of Ubiquitin" 94.74 72 100.00 100.00 8.52e-43 PDB 2MUR "Solution Structure Of The Human Faap20 Ubz-ubiquitin Complex" 100.00 78 100.00 100.00 5.38e-46 PDB 2MWS "Structure Of The Complex Of Ubiquitin And The Ubiquitin-like (ubl) Domain Of Ddi1" 100.00 76 98.68 98.68 4.40e-45 PDB 2N2K "Ensemble Structure Of The Closed State Of Lys63-linked Diubiquitin In The Absence Of A Ligand" 93.42 71 100.00 100.00 6.21e-42 PDB 2NR2 "The Mumo (Minimal Under-Restraining Minimal Over- Restraining) Method For The Determination Of Native States Ensembles Of Prote" 100.00 76 100.00 100.00 6.54e-46 PDB 2O6V "Crystal Structure And Solution Nmr Studies Of Lys48-Linked Tetraubiquitin At Neutral Ph" 100.00 76 100.00 100.00 6.54e-46 PDB 2OJR "Structure Of Ubiquitin Solved By Sad Using The Lanthanide- Binding Tag" 100.00 111 100.00 100.00 2.29e-45 PDB 2OOB "Crystal Structure Of The Uba Domain From Cbl-B Ubiquitin Ligase In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2PE9 "Nmr Based Structure Of The Open Conformation Of Lys48- Linked Di-Ubiquitin Using Experimental Global Rotational Diffusion Tenso" 100.00 76 100.00 100.00 6.54e-46 PDB 2PEA "Nmr Based Structure Of The Closed Conformation Of Lys48- Linked Di-Ubiquitin Using Experimental Global Rotational Diffusion Ten" 100.00 76 100.00 100.00 6.54e-46 PDB 2QHO "Crystal Structure Of The Uba Domain From Edd Ubiquitin Ligase In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2RR9 "The Solution Structure Of The K63-Ub2:tuims Complex" 100.00 76 100.00 100.00 6.54e-46 PDB 2RSU "Alternative Structure Of Ubiquitin" 100.00 76 98.68 98.68 3.54e-45 PDB 2RU6 "The Pure Alternative State Of Ubiquitin" 100.00 76 98.68 98.68 3.54e-45 PDB 2W9N "Crystal Structure Of Linear Di-Ubiquitin" 98.68 152 100.00 100.00 5.62e-44 PDB 2WDT "Crystal Structure Of Plasmodium Falciparum Uchl3 In Complex With The Suicide Inhibitor Ubvme" 98.68 76 100.00 100.00 4.40e-45 PDB 2WWZ "Tab2 Nzf Domain In Complex With Lys63-Linked Di-Ubiquitin, P212121" 100.00 76 100.00 100.00 6.54e-46 PDB 2WX0 "Tab2 Nzf Domain In Complex With Lys63-Linked Di-Ubiquitin, P21" 100.00 76 100.00 100.00 6.54e-46 PDB 2WX1 "Tab2 Nzf Domain In Complex With Lys63-Linked Tri-Ubiquitin, P212121" 100.00 76 100.00 100.00 6.54e-46 PDB 2XBB "Nedd4 Hect:ub Complex" 100.00 76 100.00 100.00 6.54e-46 PDB 2XEW "Crystal Structure Of K11-Linked Diubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2XK5 "Crystal Structure Of K6-Linked Diubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2Y5B "Structure Of Usp21 In Complex With Linear Diubiquitin-Aldehyde" 98.68 152 100.00 100.00 4.15e-44 PDB 2Z59 "Complex Structures Of Mouse Rpn13 (22-130aa) And Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 2ZCB "Crystal Structure Of Ubiquitin P37aP38A" 100.00 76 97.37 97.37 3.44e-44 PDB 2ZCC "Ubiquitin Crystallized Under High Pressure" 100.00 76 100.00 100.00 6.54e-46 PDB 2ZNV "Crystal Structure Of Human Amsh-Lp Dub Domain In Complex With Lys63-Linked Ubiquitin Dimer" 100.00 77 100.00 100.00 7.42e-46 PDB 2ZVN "Nemo Cozi Domain Incomplex With Diubiquitin In P212121 Space Group" 100.00 154 100.00 100.00 5.98e-45 PDB 2ZVO "Nemo Cozi Domain In Complex With Diubiquitin In C2 Space Group" 100.00 154 100.00 100.00 5.98e-45 PDB 3A1Q "Crystal Structure Of The Mouse Rap80 Uims In Complex With Lys63-Linked Di-Ubiquitin" 100.00 77 100.00 100.00 7.42e-46 PDB 3A33 "Ubch5b~ubiquitin Conjugate" 100.00 76 100.00 100.00 6.54e-46 PDB 3A9J "Crystal Structure Of The Mouse Tab2-Nzf In Complex With Lys63-Linked Di-Ubiquitin" 100.00 77 100.00 100.00 7.42e-46 PDB 3A9K "Crystal Structure Of The Mouse Tab3-Nzf In Complex With Lys63-Linked Di-Ubiquitin" 100.00 77 100.00 100.00 7.42e-46 PDB 3AI5 "Crystal Structure Of Yeast Enhanced Green Fluorescent Protein- Ubiquitin Fusion Protein" 97.37 307 100.00 100.00 1.64e-41 PDB 3ALB "Cyclic Lys48-Linked Tetraubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 3AUL "Crystal Structure Of Wild-Type Lys48-Linked Diubiquitin In An Open Conformation" 100.00 76 100.00 100.00 6.54e-46 PDB 3AXC "Crystal Structure Of Linear Diubiquitin" 100.00 154 100.00 100.00 5.98e-45 PDB 3B08 "Crystal Structure Of The Mouse Hoil1-l-nzf In Complex With Linear Di- Ubiquitin" 100.00 152 100.00 100.00 6.43e-45 PDB 3B0A "Crystal Structure Of The Mouse Hoil1-l-nzf In Complex With Linear Di- Ubiquitin" 100.00 152 100.00 100.00 6.43e-45 PDB 3BY4 "Structure Of Ovarian Tumor (Otu) Domain In Complex With Ubiquitin" 98.68 75 100.00 100.00 4.28e-45 PDB 3C0R "Structure Of Ovarian Tumor (Otu) Domain In Complex With Ubiquitin" 98.68 75 100.00 100.00 4.28e-45 PDB 3DVG "Crystal Structure Of K63-Specific Fab Apu.3a8 Bound To K63-Linked Di- Ubiquitin" 100.00 80 100.00 100.00 8.24e-46 PDB 3DVN "Crystal Structure Of K63-specific Fab Apu2.16 Bound To K63-linked Di- Ubiquitin" 100.00 80 100.00 100.00 8.24e-46 PDB 3EEC "X-Ray Structure Of Human Ubiquitin Cd(Ii) Adduct" 100.00 76 100.00 100.00 6.54e-46 PDB 3EFU "X-Ray Structure Of Human Ubiquitin-Hg(Ii) Adduct" 100.00 76 100.00 100.00 6.54e-46 PDB 3EHV "X-Ray Structure Of Human Ubiquitin Zn(Ii) Adduct" 100.00 76 100.00 100.00 6.54e-46 PDB 3H1U "Structure Of Ubiquitin In Complex With Cd Ions" 100.00 76 100.00 100.00 6.54e-46 PDB 3H7P "Crystal Structure Of K63-Linked Di-Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 3H7S "Crystal Structures Of K63-Linked Di- And Tri-Ubiquitin Reveal A Highly Extended Chain Architecture" 100.00 76 98.68 98.68 2.81e-43 PDB 3HM3 "The Structure And Conformation Of Lys-63 Linked Tetra-Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 3I3T "Crystal Structure Of Covalent Ubiquitin-usp21 Complex" 98.68 75 100.00 100.00 4.28e-45 PDB 3IFW "Crystal Structure Of The S18y Variant Of Ubiquitin Carboxy T Hydrolase L1 Bound To Ubiquitin Vinylmethylester." 98.68 75 100.00 100.00 4.28e-45 PDB 3IHP "Covalent Ubiquitin-Usp5 Complex" 98.68 75 100.00 100.00 4.28e-45 PDB 3JSV "Crystal Structure Of Mouse Nemo Cozi In Complex With Lys63- Linked Di-Ubiquitin" 100.00 77 100.00 100.00 7.42e-46 PDB 3JVZ E2~ubiquitin-Hect 100.00 81 100.00 100.00 7.68e-46 PDB 3JW0 E2~ubiquitin-Hect 100.00 81 100.00 100.00 7.68e-46 PDB 3K9O "The Crystal Structure Of E2-25k And Ubb+1 Complex" 98.68 96 100.00 100.00 1.27e-44 PDB 3K9P "The Crystal Structure Of E2-25k And Ubiquitin Complex" 100.00 79 100.00 100.00 6.66e-46 PDB 3KVF "Crystal Structure Of The I93m Mutant Of Ubiquitin Carboxy Te Hydrolase L1 Bound To Ubiquitin Vinylmethylester" 98.68 75 100.00 100.00 4.28e-45 PDB 3KW5 "Crystal Structure Of Ubiquitin Carboxy Terminal Hydrolase L1 Ubiquitin Vinylmethylester" 98.68 75 100.00 100.00 4.28e-45 PDB 3LDZ "Crystal Structure Of Human Stam1 Vhs Domain In Complex With Ubiquitin" 96.05 73 100.00 100.00 1.79e-43 PDB 3M3J "A New Crystal Form Of Lys48-Linked Diubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 3MHS "Structure Of The Saga Ubp8SGF11SUS1SGF73 DUB MODULE BOUND Ubiquitin Aldehyde" 98.68 76 100.00 100.00 4.40e-45 PDB 3MTN "Usp21 In Complex With A Ubiquitin-based, Usp21-specific Inhibitor" 88.16 85 98.51 98.51 1.47e-37 PDB 3N30 "Crystal Structure Of Cubic Zn3-Hub (Human Ubiquitin) Adduct" 100.00 76 100.00 100.00 6.54e-46 PDB 3N32 "The Crystal Structure Of Human Ubiquitin Adduct With Zeise's Salt" 100.00 76 100.00 100.00 6.54e-46 PDB 3NHE "High Resolution Structure (1.26a) Of Usp2a In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 3NOB "Structure Of K11-linked Di-ubiquitin" 100.00 78 100.00 100.00 5.38e-46 PDB 3NS8 "Crystal Structure Of An Open Conformation Of Lys48-Linked Diubiquitin At Ph 7.5" 100.00 76 100.00 100.00 6.54e-46 PDB 3O65 "Crystal Structure Of A Josephin-Ubiquitin Complex: Evolutionary Restraints On Ataxin-3 Deubiquitinating Activity" 98.68 76 100.00 100.00 4.40e-45 PDB 3OFI "Crystal Structure Of Human Insulin-Degrading Enzyme In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 3OJ3 "Crystal Structure Of The A20 Znf4 And Ubiquitin Complex" 100.00 79 100.00 100.00 6.66e-46 PDB 3OJ4 "Crystal Structure Of The A20 Znf4, Ubiquitin And Ubch5a Complex" 100.00 79 100.00 100.00 6.66e-46 PDB 3ONS "Crystal Structure Of Human Ubiquitin In A New Crystal Form" 94.74 72 100.00 100.00 8.52e-43 PDB 3PHD "Crystal Structure Of Human Hdac6 In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 3PHW "Otu Domain Of Crimean Congo Hemorrhagic Fever Virus In Complex With Ubiquitin" 98.68 75 100.00 100.00 4.28e-45 PDB 3PRM "Structural Analysis Of A Viral Otu Domain Protease From The Crimean- Congo Hemorrhagic Fever Virus In Complex With Human Ubiqui" 98.68 75 100.00 100.00 4.28e-45 PDB 3PRP "Structural Analysis Of A Viral Otu Domain Protease From The Crimean- Congo Hemorrhagic Fever Virus In Complex With Human Ubiqui" 98.68 75 100.00 100.00 4.28e-45 PDB 3PT2 "Structure Of A Viral Otu Domain Protease Bound To Ubiquitin" 98.68 75 100.00 100.00 4.28e-45 PDB 3PTF "X-Ray Structure Of The Non-Covalent Complex Between Ubch5a And Ubiquitin" 100.00 79 100.00 100.00 6.66e-46 PDB 3Q3F "Engineering Domain-Swapped Binding Interfaces By Mutually Exclusive Folding: Insertion Of Ubiquitin Into Position 103 Of Barnas" 98.68 189 100.00 100.00 5.35e-44 PDB 3RUL "New Strategy To Analyze Structures Of Glycopeptide-Target Complexes" 98.68 79 100.00 100.00 3.97e-45 PDB 3TBL "Structure Of Mono-ubiquitinated Pcna: Implications For Dna Polymerase Switching And Okazaki Fragment Maturation" 100.00 76 100.00 100.00 6.54e-46 PDB 3TMP "The Catalytic Domain Of Human Deubiquitinase Duba In Complex With Ubiquitin Aldehyde" 98.68 76 100.00 100.00 4.40e-45 PDB 3U30 "Crystal Structure Of A Linear-Specific Ubiquitin Fab Bound To Linear Ubiquitin" 100.00 172 100.00 100.00 6.89e-45 PDB 3UGB "Ubch5c~ubiquitin Conjugate" 100.00 76 100.00 100.00 6.54e-46 PDB 3VDZ "Tailoring Encodable Lanthanide-Binding Tags As Mri Contrast Agents: Xq-Dse3-Ubiquitin At 2.4 Angstroms" 100.00 111 100.00 100.00 1.47e-45 PDB 3VFK "The Structure Of Monodechloro-teicoplanin In Complex With Its Ligand, Using Ubiquitin As A Ligand Carrier" 98.68 79 100.00 100.00 3.97e-45 PDB 3VHT "Crystal Structure Of Gfp-Wrnip1 Ubz Domain Fusion Protein In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 3VUW "Crystal Structure Of A20 Zf7 In Complex With Linear Ubiquitin, Form I" 100.00 76 100.00 100.00 6.54e-46 PDB 3VUX "Crystal Structure Of A20 Zf7 In Complex With Linear Ubiquitin, Form Ii" 100.00 76 100.00 100.00 6.54e-46 PDB 3VUY "Crystal Structure Of A20 Zf7 In Complex With Linear Tetraubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 3WWQ "Crystal Structure Of Faap20 Ubz Domain In Complex With Lys63-linked Diubiquitin" 100.00 77 100.00 100.00 7.42e-46 PDB 3WXE "Crystal Structure Of Cyld Usp Domain (c596s) In Complex With Met1- Linked Diubiquitin" 94.74 148 100.00 100.00 9.19e-42 PDB 3WXF "Crystal Structure Of Cyld Usp Domain (c596s E674q) In Complex With Met1-linked Diubiquitin" 94.74 148 100.00 100.00 9.19e-42 PDB 3WXG "Crystal Structure Of Cyld Usp Domain (c596a) In Complex With Lys63- Linked Diubiquitin" 94.74 72 100.00 100.00 8.52e-43 PDB 3ZLZ "Lys6-linked Tri-ubiquitin" 100.00 76 98.68 100.00 1.56e-45 PDB 3ZNH "Crimean Congo Hemorrhagic Fever Virus Otu Domain In Complex With Ubiquitin-propargyl." 98.68 76 100.00 100.00 4.40e-45 PDB 3ZNI "Structure Of Phosphotyr363-cbl-b - Ubch5b-ub - Zap-70 Peptide Complex" 100.00 81 100.00 100.00 7.68e-46 PDB 3ZNZ "Crystal Structure Of Otulin Otu Domain (c129a) In Complex With Met1-di Ubiquitin" 100.00 152 100.00 100.00 6.43e-45 PDB 4A18 "T.Thermophila 60s Ribosomal Subunit In Complex With Initiation Factor 6. This File Contains 26s Rrna And Proteins Of Molecule 1" 100.00 129 97.37 98.68 2.69e-44 PDB 4A19 "T.Thermophila 60s Ribosomal Subunit In Complex With Initiation Factor 6. This File Contains 26s Rrna And Proteins Of Molecule 2" 100.00 129 97.37 98.68 2.69e-44 PDB 4A1B "T.Thermophila 60s Ribosomal Subunit In Complex With Initiation Factor 6. This File Contains 26s Rrna And Proteins Of Molecule 3" 100.00 129 97.37 98.68 2.69e-44 PDB 4A1D "T.Thermophila 60s Ribosomal Subunit In Complex With Initiation Factor 6. This File Contains 26s Rrna And Proteins Of Molecule 4" 100.00 129 97.37 98.68 2.69e-44 PDB 4ADX "The Cryo-em Structure Of The Archaeal 50s Ribosomal Subunit In Complex With Initiation Factor 6" 100.00 129 97.37 98.68 2.69e-44 PDB 4AP4 "Rnf4 - Ubch5a - Ubiquitin Heterotrimeric Complex" 100.00 80 100.00 100.00 6.85e-46 PDB 4AUQ "Structure Of Birc7-Ubch5b-Ub Complex." 100.00 81 100.00 100.00 7.68e-46 PDB 4BBN "Nedd4 Hect-ub:ub Complex" 100.00 76 100.00 100.00 6.54e-46 PDB 4BOS "Structure Of Otud2 Otu Domain In Complex With Ubiquitin K11- Linked Peptide" 100.00 76 100.00 100.00 6.54e-46 PDB 4BOZ "Structure Of Otud2 Otu Domain In Complex With K11-linked Di Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 4BVU "Structure Of Shigella Effector Ospg In Complex With Host Ubch5c-ubiquitin Conjugate" 100.00 76 100.00 100.00 6.54e-46 PDB 4CXC "Regulation Of The Mammalian Elongation Cycle By 40s Subunit Rolling: A Eukaryotic-specific Ribosome Rearrangement" 100.00 156 100.00 100.00 7.65e-46 PDB 4CXD "Regulation Of The Mammalian Elongation Cycle By 40s Subunit Rolling: A Eukaryotic-specific Ribosome Rearrangement" 100.00 128 100.00 100.00 4.55e-46 PDB 4D5L "Cryo-em Structures Of Ribosomal 80s Complexes With Termination Factors And Cricket Paralysis Virus Ires Reveal The Ires In The " 100.00 156 100.00 100.00 7.65e-46 PDB 4D61 "Cryo-em Structures Of Ribosomal 80s Complexes With Termination Factors And Cricket Paralysis Virus Ires Reveal The Ires In The " 100.00 156 100.00 100.00 7.65e-46 PDB 4DDG "Crystal Structure Of Human Otub1UBCH5B~UBUB" 100.00 76 100.00 100.00 6.54e-46 PDB 4DDI "Crystal Structure Of Human Otub1UBCH5B~UBUB" 100.00 76 100.00 100.00 6.54e-46 PDB 4DHJ "The Structure Of A Ceotub1 Ubiquitin Aldehyde Ubc13~ub Complex" 98.68 76 100.00 100.00 3.99e-45 PDB 4DHZ "The Structure Of HCEOTUB1-Ubiquitin Aldehyde-Ubc13~ub" 98.68 76 100.00 100.00 3.99e-45 PDB 4FJV "Crystal Structure Of Human Otubain2 And Ubiquitin Complex" 100.00 86 100.00 100.00 9.86e-46 PDB 4HXD "Diversity Of Ubiquitin And Isg15 Specificity Amongst Nairoviruses Viral Ovarian Tumor Domain Proteases" 98.68 75 100.00 100.00 4.28e-45 PDB 4I6N "Crystal Structure Of Trichinella Spiralis Uch37 Catalytic Domain Bound To Ubiquitin Vinyl Methyl Ester" 97.37 75 100.00 100.00 4.54e-44 PDB 4IG7 "Crystal Structure Of Trichinella Spiralis Uch37 Bound To Ubiquitin Vinyl Methyl Ester" 98.68 75 100.00 100.00 4.28e-45 PDB 4IUM "Equine Arteritis Virus Papain-like Protease 2 (plp2) Covalently Bound To Ubiquitin" 98.68 76 100.00 100.00 4.40e-45 PDB 4JIO "Bro1 V Domain And Ubiquitin" 100.00 76 98.68 98.68 4.85e-45 PDB 4JQW "Crystal Structure Of A Complex Of Nod1 Card And Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 4K1R "Crystal Structure Of Schizosaccharomyces Pombe Sst2 Catalytic Domain And Ubiquitin" 100.00 81 100.00 100.00 1.15e-45 PDB 4K7S "Crystal Structure Of Zn2-hub (human Ubiquitin) Adduct From A Solution 35 Mm Zinc Acetate/1.3 Mm Hub" 100.00 76 100.00 100.00 6.54e-46 PDB 4K7U "Crystal Structure Of Zn2.3-hub (human Ubiquitin) Adduct From A Solution 70 Mm Zinc Acetate/1.3 Mm Hub" 100.00 76 100.00 100.00 6.54e-46 PDB 4K7W "Crystal Structure Of Zn3-hub(human Ubiquitin) Adduct From A Solution 100 Mm Zinc Acetate/1.3 Mm Hub" 100.00 76 100.00 100.00 6.54e-46 PDB 4KSK "Gumby/fam105b In Complex With Ubiquitin" 100.00 80 100.00 100.00 6.85e-46 PDB 4KSL "Gumby/fam105b In Complex With Linear Di-ubiquitin" 100.00 156 100.00 100.00 6.34e-45 PDB 4KZX "Rabbit 40s Ribosomal Subunit In Complex With Eif1." 100.00 156 100.00 100.00 7.65e-46 PDB 4KZY "Rabbit 40s Ribosomal Subunit In Complex With Eif1 And Eif1a." 100.00 156 100.00 100.00 7.65e-46 PDB 4KZZ "Rabbit 40s Ribosomal Subunit In Complex With Mrna, Initiator Trna And Eif1a" 100.00 156 100.00 100.00 7.65e-46 PDB 4LCD "Structure Of An Rsp5xubxsna3 Complex: Mechanism Of Ubiquitin Ligation And Lysine Prioritization By A Hect E3" 97.37 83 100.00 100.00 1.79e-44 PDB 4LDT "The Structure Of H/ceotub1-ubiquitin Aldehyde-ubch5b~ub" 100.00 76 100.00 100.00 6.54e-46 PDB 4LJO "Structure Of An Active Ligase (hoip)/ubiquitin Transfer Complex" 100.00 76 100.00 100.00 6.54e-46 PDB 4LJP "Structure Of An Active Ligase (hoip-h889a)/ubiquitin Transfer Complex" 100.00 76 100.00 100.00 6.54e-46 PDB 4M0W "Crystal Structure Of Sars-cov Papain-like Protease C112s Mutant In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 4MDK "Cdc34-ubiquitin-cc0651 Complex" 100.00 80 100.00 100.00 6.85e-46 PDB 4MM3 "Crystal Structure Of Sars-cov Papain-like Protease Plpro In Complex With Ubiquitin Aldehyde" 98.68 76 100.00 100.00 4.40e-45 PDB 4MSM "Crystal Structure Of Schizosaccharomyces Pombe Amsh-like Protease Sst2 E286a Mutant Bound To Ubiquitin" 100.00 81 100.00 100.00 1.15e-45 PDB 4MSQ "Crystal Structure Of Schizosaccharomyces Pombe Amsh-like Protease Sst2 Catalytic Domain Bound To Ubiquitin" 100.00 81 100.00 100.00 1.15e-45 PDB 4NQK "Structure Of An Ubiquitin Complex" 100.00 79 100.00 100.00 1.20e-45 PDB 4NQL "The Crystal Structure Of The Dub Domain Of Amsh Orthologue, Sst2 From S. Pombe, In Complex With Lysine 63-linked Diubiquitin" 100.00 77 100.00 100.00 7.42e-46 PDB 4P4H "Caught-in-action Signaling Complex Of Rig-i 2card Domain And Mavs Card Domain" 100.00 79 100.00 100.00 1.20e-45 PDB 4PIG "Crystal Structure Of The Ubiquitin K11s Mutant" 100.00 76 98.68 98.68 2.81e-45 PDB 4PIH "X-ray Crystal Structure Of The K33s Mutant Of Ubiquitin" 100.00 76 98.68 98.68 2.81e-45 PDB 4PIJ "X-ray Crystal Structure Of The K11s/k63s Double Mutant Of Ubiquitin" 98.68 75 97.33 97.33 7.90e-44 PDB 4PQT "Insights Into The Mechanism Of Deubiquitination By Jamm Deubiquitinases From Co-crystal Structures Of Enzyme With Substrate And" 100.00 81 100.00 100.00 1.15e-45 PDB 4R62 "Structure Of Rad6~ub" 100.00 78 100.00 100.00 6.20e-46 PDB 4RF0 "Crystal Structure Of The Middle-east Respiratory Syndrome Coronavirus Papain-like Protease In Complex With Ubiquitin (space Gro" 98.68 75 100.00 100.00 4.28e-45 PDB 4RF1 "Crystal Structure Of The Middle-east Respiratory Syndrome Coronavirus Papain-like Protease In Complex With Ubiquitin (space Gro" 98.68 75 100.00 100.00 4.28e-45 PDB 4S1Z "Crystal Structure Of Trabid Nzf1 In Complex With K29 Linked Di- Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 4S22 "Crystal Structure Of K29 Linked Di-ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 4UEL "Uch-l5 In Complex With Ubiquitin-propargyl Bound To The Rpn13 Deubad Domain" 98.68 76 100.00 100.00 4.40e-45 PDB 4UF6 "Uch-l5 In Complex With Ubiquitin-propargyl Bound To An Activating Fragment Of Ino80g" 98.68 76 100.00 100.00 4.40e-45 PDB 4UN2 "Crystal Structure Of The Uba Domain Of Dsk2 In Complex With Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 4UPX "Mammalian 80s Hcv-ires Initiation Complex With Eif5b Pre-like State" 100.00 128 100.00 100.00 4.55e-46 PDB 4UQ1 "Mammalian 80s Hcv-ires Initiation Complex With Eif5b Post-like State" 100.00 128 100.00 100.00 4.55e-46 PDB 4UQ4 "Mammalian 80s Hcv-ires Initiation Complex With Eif5b Post-like State" 100.00 156 100.00 100.00 7.65e-46 PDB 4UQ5 "Mammalian 80s Hcv-ires Initiation Complex With Eif5b Pre-like State" 100.00 156 100.00 100.00 7.65e-46 PDB 4V3K "Rnf38-ubch5b-ub Complex" 100.00 81 100.00 100.00 7.68e-46 PDB 4V3L "Rnf38-ub-ubch5b-ub Complex" 100.00 81 100.00 100.00 7.68e-46 PDB 4W20 "Structure Of The Mammalian 60s Ribosomal Subunit (this Entry Contains The Large Ribosomal Proteins)" 100.00 128 100.00 100.00 4.55e-46 PDB 4W22 "Structure Of The 80s Mammalian Ribosome Bound To Eef2 (this Entry Contains The Large Ribosomal Subunit Proteins)" 100.00 128 100.00 100.00 4.55e-46 PDB 4W23 "Structure Of The 80s Mammalian Ribosome Bound To Eef2 (this Entry Contains The Small Ribosomal Subunit)" 100.00 156 100.00 100.00 7.65e-46 PDB 4W25 "Structure Of The Idle Mammalian Ribosome-sec61 Complex (this Entry Contains The Large Ribosomal Subunit Proteins)" 100.00 128 100.00 100.00 4.55e-46 PDB 4W27 "Structure Of The Translating Mammalian Ribosome-sec61 Complex (this Entry Contains The Large Ribosomal Subunit Proteins)" 100.00 128 100.00 100.00 4.55e-46 PDB 4W28 "Structure Of The Translating Mammalian Ribosome-sec61 Complex (this Entry Contains The Small Ribosomal Subunit)" 100.00 156 100.00 100.00 7.65e-46 PDB 4WHV Rnf8/ubc13c87k~ub 100.00 83 100.00 100.00 1.61e-45 PDB 4WLR "Crystal Structure Of Much37-hrpn13 Ctd-hub Complex" 100.00 76 100.00 100.00 6.54e-46 PDB 4WUR "The Crystal Structure Of The Mers-cov Papain-like Protease (c111s) With Human Ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 4WZP "Ser65 Phosphorylated Ubiquitin, Major Conformation" 100.00 76 98.68 98.68 4.64e-45 PDB 4XKL "Crystal Structure Of Ndp52 Zf2 In Complex With Mono-ubiquitin" 100.00 80 100.00 100.00 1.02e-45 PDB 4XOF "Observing The Overall Rocking Motion Of A Protein In A Crystal - Orthorhombic Ubiquitin Crystals Without Zinc." 100.00 76 100.00 100.00 6.54e-46 PDB 4XOK "Observing The Overall Rocking Motion Of A Protein In A Crystal." 100.00 76 100.00 100.00 6.54e-46 PDB 4XOL "Observing The Overall Rocking Motion Of A Protein In A Crystal - Cubic Ubiquitin Crystals." 100.00 76 100.00 100.00 6.54e-46 PDB 4XYZ "Crystal Structure Of K33 Linked Di-ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 4Y1H "Crystal Structure Of K33 Linked Tri-ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 4Z9S "Non-covalent Assembly Of Monoubiquitin That Mimics K11 Poly-ubiquitin" 100.00 76 100.00 100.00 6.54e-46 PDB 4ZFR "Catalytic Domain Of Sst2 F403a Mutant Bound To Ubiquitin" 100.00 81 100.00 100.00 1.15e-45 PDB 4ZFT "Catalytic Domain Of Sst2 F403w Mutant Bound To Ubiquitin" 100.00 81 100.00 100.00 1.15e-45 PDB 4ZPZ "Crystal Structure Of Semi-synthetic Ubiquitin With Phospho-ser65 And Ala46cys" 100.00 76 97.37 97.37 1.81e-44 PDB 5A5B "Structure Of The 26s Proteasome-ubp6 Complex" 98.68 76 100.00 100.00 4.40e-45 PDB 5AF4 "Structure Of Lys33-linked Diub" 100.00 76 98.68 100.00 1.56e-45 PDB 5AF5 "Structure Of Lys33-linked Triub S.g. P 212121" 96.05 73 98.63 100.00 5.42e-43 PDB 5AF6 "Structure Of Lys33-linked Diub Bound To Trabid Nzf1" 100.00 76 98.68 100.00 1.56e-45 PDB 5AIT "A Complex Of Of Rnf4-ring Domain, Ubev2, Ubc13-ub (isopeptide Crosslink)" 100.00 76 100.00 100.00 6.54e-46 PDB 5AIU "A Complex Of Rnf4-ring Domain, Ubc13-ub (isopeptide Crosslink)" 100.00 76 100.00 100.00 6.54e-46 PDB 5CAW "Structure Of Pediculus Humanus Parkin Bound To Phospho-ubiquitin" 98.68 76 98.67 98.67 2.77e-44 DBJ BAA03983 "polyubiquitin [Rattus norvegicus]" 100.00 305 100.00 100.00 3.25e-43 DBJ BAA09860 "polyubiquitin [Homo sapiens]" 100.00 611 98.68 98.68 1.53e-40 DBJ BAA11842 "ubiquitin [Cavia porcellus]" 100.00 311 100.00 100.00 3.52e-43 DBJ BAA11843 "ubiquitin extention protein [Cavia porcellus]" 100.00 156 100.00 100.00 7.65e-46 DBJ BAA23486 "polyubiquitin [Homo sapiens]" 100.00 609 98.68 98.68 8.23e-41 EMBL CAA25706 "unnamed protein product [Saccharomyces cerevisiae]" 50.00 191 100.00 100.00 4.38e-16 EMBL CAA26488 "unnamed protein product [Gallus gallus]" 100.00 157 98.68 98.68 3.90e-44 EMBL CAA28495 "ubiquitin [Homo sapiens]" 100.00 229 100.00 100.00 5.18e-44 EMBL CAA30183 "unnamed protein product [Dictyostelium discoideum]" 100.00 128 97.37 97.37 7.90e-44 EMBL CAA30815 "unnamed protein product [Cricetulus sp.]" 93.42 223 100.00 100.00 5.43e-40 GB AAA02769 "polyprotein [Bovine viral diarrhea virus 1-Osloss]" 98.68 3975 97.33 100.00 2.69e-39 GB AAA28154 "polyubiquitin [Caenorhabditis elegans]" 100.00 838 97.37 98.68 1.06e-39 GB AAA28997 "ubiquitin [Drosophila melanogaster]" 100.00 231 100.00 100.00 4.85e-44 GB AAA28998 "ubiquitin-hybrid protein precursor [Drosophila melanogaster]" 100.00 156 100.00 100.00 1.42e-45 GB AAA28999 "ubiquitin, partial [Drosophila melanogaster]" 100.00 76 100.00 100.00 6.54e-46 PIR I50437 "polyubiquitin 4 - chicken [Gallus gallus]" 100.00 305 100.00 100.00 3.25e-43 PIR I51568 "polyubiquitin - African clawed frog (fragment)" 100.00 167 100.00 100.00 1.00e-44 PIR I65237 "ubiquitin / ribosomal protein L40, cytosolic [validated] - rat" 100.00 128 100.00 100.00 4.55e-46 PIR JN0790 "ubiquitin/ribosomal protein CEP52 fusion protein - Leishmania major" 100.00 128 97.37 98.68 3.43e-45 PIR S13928 "ubiquitin precursor - chicken [Gallus gallus]" 100.00 229 100.00 100.00 5.35e-44 PRF 0412265A ubiquitin 98.68 75 98.67 98.67 1.80e-44 PRF 1101405A "ubiquitin precursor" 50.00 191 100.00 100.00 4.33e-16 PRF 1212243A "ubiquitin S1" 100.00 76 100.00 100.00 6.54e-46 PRF 1212243B "ubiquitin S5" 92.11 77 98.57 98.57 1.12e-40 PRF 1212243C "ubiquitin S3" 100.00 76 100.00 100.00 6.54e-46 REF NP_001005123 "ubiquitin-60S ribosomal protein L40 [Xenopus (Silurana) tropicalis]" 100.00 128 100.00 100.00 4.55e-46 REF NP_001006688 "ubiquitin C [Xenopus (Silurana) tropicalis]" 100.00 609 100.00 100.00 1.45e-41 REF NP_001009117 "polyubiquitin-B [Pan troglodytes]" 100.00 229 100.00 100.00 5.18e-44 REF NP_001009202 "polyubiquitin-B [Ovis aries]" 100.00 305 98.68 100.00 5.72e-43 REF NP_001009286 "ubiquitin-60S ribosomal protein L40 [Ovis aries]" 100.00 128 100.00 100.00 4.55e-46 SP P0C273 "RecName: Full=Ubiquitin-60S ribosomal protein L40; AltName: Full=Ubiquitin A-52 residue ribosomal protein fusion product 1; Con" 100.00 128 100.00 100.00 4.55e-46 SP P0C275 "RecName: Full=Ubiquitin-60S ribosomal protein L40; AltName: Full=Ubiquitin A-52 residue ribosomal protein fusion product 1; Con" 100.00 128 100.00 100.00 4.55e-46 SP P0C276 "RecName: Full=Ubiquitin-60S ribosomal protein L40; AltName: Full=Ubiquitin A-52 residue ribosomal protein fusion product 1; Con" 100.00 128 100.00 100.00 4.55e-46 SP P0CG47 "RecName: Full=Polyubiquitin-B; Contains: RecName: Full=Ubiquitin; Flags: Precursor" 100.00 229 100.00 100.00 5.18e-44 SP P0CG48 "RecName: Full=Polyubiquitin-C; Contains: RecName: Full=Ubiquitin; Flags: Precursor" 100.00 685 100.00 100.00 2.13e-41 TPD FAA00319 "TPA: polyubiquitin [Cryptococcus neoformans var. neoformans B-3501A]" 100.00 456 97.37 98.68 1.11e-40 TPE CEL68433 "TPA: ubiquitin / ribosomal protein CEP52 fusion protein, putative [Neospora caninum Liverpool]" 100.00 129 98.68 100.00 1.60e-45 TPE CEL70397 "TPA: Ubiquitin, related [Neospora caninum Liverpool]" 100.00 535 98.68 100.00 3.17e-41 TPE CEL75964 "TPA: ubiquitin / ribosomal protein CEP52 fusion protein, putative [Toxoplasma gondii VEG]" 100.00 129 98.68 100.00 1.60e-45 TPE CEL78064 "TPA: polyubiquitin, putative [Toxoplasma gondii VEG]" 100.00 307 98.68 100.00 1.16e-42 TPG DAA18802 "TPA: polyubiquitin [Bos taurus]" 100.00 305 100.00 100.00 3.43e-43 TPG DAA20663 "TPA: ubiquitin C [Bos taurus]" 98.68 314 98.67 100.00 1.30e-41 TPG DAA20672 "TPA: ubiquitin B-like [Bos taurus]" 100.00 77 98.68 98.68 4.88e-45 TPG DAA24675 "TPA: 40S ribosomal protein S27a [Bos taurus]" 100.00 156 100.00 100.00 7.65e-46 TPG DAA28295 "TPA: ubiquitin and ribosomal protein L40 [Bos taurus]" 100.00 128 100.00 100.00 4.55e-46 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $Ubiquitin Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $Ubiquitin 'recombinant technology' . Escherichia coli . pET-21b(+) stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details 'Ubiquitin-13C,15N at 258K and 2500 bar' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $Ubiquitin 1.0 mM '[U-100% 13C; U-100% 15N]' D2O 10 % [U-2H] 'potassium phosphate' 10 mM 'natural abundance' 'sodium azide' 0.02 % 'natural abundance' H2O 90 % 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ save_NMRView _Saveframe_category software _Name NMRView _Version . loop_ _Vendor _Address _Electronic_address 'Johnson, One Moon Scientific' . . stop_ loop_ _Task 'data analysis' stop_ _Details . save_ save_PIPP _Saveframe_category software _Name PIPP _Version . loop_ _Vendor _Address _Electronic_address Garrett . . stop_ loop_ _Task 'peak picking' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DRX _Field_strength 800 _Details . save_ save_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DRX _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_HNCO_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO' _Sample_label $sample_1 save_ save_3D_HNCA_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCA' _Sample_label $sample_1 save_ save_3D_CBCA(CO)NH_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $sample_1 save_ save_3D_1H-15N_NOESY_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-15N NOESY' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details 'Ubiquitin-13C,15N at 258K and 2500 bar' loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 10 . mM pH 6.5 . pH pressure 2500 . bar temperature 258 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio TSP C 13 'methyl protons' ppm 0.00 . indirect . . . 0.251449530 TSP H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 TSP N 15 'methyl protons' ppm 0.00 . indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details 'Folded state of ubiquitin at 258K and 2500 bar' loop_ _Experiment_label '2D 1H-15N HSQC' '3D HNCO' '3D HNCA' '3D CBCA(CO)NH' '3D 1H-15N NOESY' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name Ubiquitin_folded _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 1 1 MET C C 170.115 0.040 1 2 1 1 MET CA C 54.395 0.040 1 3 1 1 MET CB C 32.985 0.040 1 4 2 2 GLN H H 9.138 0.005 1 5 2 2 GLN C C 176.025 0.040 1 6 2 2 GLN CA C 54.405 0.040 1 7 2 2 GLN CB C 30.705 0.040 1 8 2 2 GLN N N 123.249 0.040 1 9 3 3 ILE H H 8.228 0.005 1 10 3 3 ILE C C 172.325 0.040 1 11 3 3 ILE CA C 59.635 0.040 1 12 3 3 ILE CB C 41.675 0.040 1 13 3 3 ILE N N 114.989 0.040 1 14 4 4 PHE H H 8.998 0.005 1 15 4 4 PHE C C 175.715 0.040 1 16 4 4 PHE CA C 54.925 0.040 1 17 4 4 PHE N N 118.659 0.040 1 18 5 5 VAL H H 9.318 0.005 1 19 5 5 VAL C C 174.715 0.040 1 20 5 5 VAL CA C 60.285 0.040 1 21 5 5 VAL CB C 34.415 0.040 1 22 5 5 VAL N N 119.899 0.040 1 23 6 6 LYS H H 8.858 0.005 1 24 6 6 LYS C C 176.905 0.040 1 25 6 6 LYS CA C 54.435 0.040 1 26 6 6 LYS N N 128.529 0.040 1 27 7 7 THR H H 9.348 0.005 1 28 7 7 THR CA C 60.755 0.040 1 29 7 7 THR N N 118.409 0.040 1 30 8 8 LEU C C 179.285 0.040 1 31 8 8 LEU CA C 57.385 0.040 1 32 8 8 LEU CB C 41.935 0.040 1 33 9 9 THR H H 7.828 0.005 1 34 9 9 THR C C 175.735 0.040 1 35 9 9 THR CA C 61.235 0.040 1 36 9 9 THR CB C 68.775 0.040 1 37 9 9 THR N N 106.649 0.040 1 38 10 10 GLY H H 7.918 0.005 1 39 10 10 GLY C C 173.775 0.040 1 40 10 10 GLY CA C 45.405 0.040 1 41 10 10 GLY N N 110.429 0.040 1 42 11 11 LYS H H 7.438 0.005 1 43 11 11 LYS C C 175.875 0.040 1 44 11 11 LYS CA C 55.805 0.040 1 45 11 11 LYS CB C 33.285 0.040 1 46 11 11 LYS N N 122.879 0.040 1 47 12 12 THR H H 9.268 0.005 1 48 12 12 THR C C 174.745 0.040 1 49 12 12 THR CA C 62.585 0.040 1 50 12 12 THR N N 122.349 0.040 1 51 13 13 ILE H H 9.538 0.005 1 52 13 13 ILE C C 174.675 0.040 1 53 13 13 ILE CA C 59.395 0.040 1 54 13 13 ILE N N 126.239 0.040 1 55 14 14 THR H H 9.128 0.005 1 56 14 14 THR C C 173.535 0.040 1 57 14 14 THR CA C 61.235 0.040 1 58 14 14 THR N N 119.779 0.040 1 59 15 15 LEU H H 9.058 0.005 1 60 15 15 LEU C C 174.825 0.040 1 61 15 15 LEU CA C 52.285 0.040 1 62 15 15 LEU N N 124.679 0.040 1 63 16 16 GLU H H 8.278 0.005 1 64 16 16 GLU C C 175.315 0.040 1 65 16 16 GLU CA C 53.745 0.040 1 66 16 16 GLU CB C 29.555 0.040 1 67 16 16 GLU N N 123.039 0.040 1 68 17 17 VAL H H 9.078 0.005 1 69 17 17 VAL C C 173.825 0.040 1 70 17 17 VAL CA C 58.355 0.040 1 71 17 17 VAL N N 117.909 0.040 1 72 18 18 GLU H H 8.428 0.005 1 73 18 18 GLU CA C 52.455 0.040 1 74 18 18 GLU N N 118.459 0.040 1 75 19 19 PRO C C 175.435 0.040 1 76 19 19 PRO CA C 65.265 0.040 1 77 19 19 PRO CB C 33.405 0.040 1 78 20 20 SER H H 7.388 0.005 1 79 20 20 SER C C 174.865 0.040 1 80 20 20 SER CA C 57.505 0.040 1 81 20 20 SER CB C 63.375 0.040 1 82 20 20 SER N N 104.219 0.040 1 83 21 21 ASP H H 8.328 0.005 1 84 21 21 ASP C C 176.185 0.040 1 85 21 21 ASP CA C 55.495 0.040 1 86 21 21 ASP N N 125.279 0.040 1 87 22 22 THR H H 8.368 0.005 1 88 22 22 THR C C 173.865 0.040 1 89 22 22 THR CA C 59.825 0.040 1 90 22 22 THR N N 110.609 0.040 1 91 23 23 ILE H H 8.828 0.005 1 92 23 23 ILE CA C 62.145 0.040 1 93 23 23 ILE N N 121.579 0.040 1 94 24 24 GLU CA C 56.825 0.040 1 95 25 25 ASN H H 8.248 0.005 1 96 25 25 ASN C C 178.425 0.040 1 97 25 25 ASN CA C 56.365 0.040 1 98 25 25 ASN CB C 38.965 0.040 1 99 25 25 ASN N N 122.729 0.040 1 100 26 26 VAL H H 8.388 0.005 1 101 26 26 VAL C C 177.685 0.040 1 102 26 26 VAL CA C 61.645 0.040 1 103 26 26 VAL N N 123.179 0.040 1 104 27 27 LYS H H 8.848 0.005 1 105 27 27 LYS CA C 58.955 0.040 1 106 27 27 LYS N N 120.069 0.040 1 107 28 28 ALA H H 8.068 0.005 1 108 28 28 ALA C C 180.225 0.040 1 109 28 28 ALA CA C 52.845 0.040 1 110 28 28 ALA N N 124.449 0.040 1 111 29 29 LYS H H 8.018 0.005 1 112 29 29 LYS CA C 59.525 0.040 1 113 29 29 LYS N N 121.239 0.040 1 114 30 30 ILE C C 177.795 0.040 1 115 31 31 GLN H H 8.848 0.005 1 116 31 31 GLN C C 179.225 0.040 1 117 31 31 GLN N N 124.019 0.040 1 118 32 32 ASP H H 8.438 0.005 1 119 32 32 ASP CA C 57.115 0.040 1 120 32 32 ASP N N 120.989 0.040 1 121 33 33 LYS C C 177.785 0.040 1 122 34 34 GLU H H 8.738 0.005 1 123 34 34 GLU C C 177.625 0.040 1 124 34 34 GLU N N 115.189 0.040 1 125 35 35 GLY H H 8.768 0.005 1 126 35 35 GLY CA C 45.885 0.040 1 127 35 35 GLY N N 110.689 0.040 1 128 38 38 PRO C C 178.135 0.040 1 129 39 39 ASP H H 8.918 0.005 1 130 39 39 ASP N N 115.639 0.040 1 131 40 40 GLN H H 8.038 0.005 1 132 40 40 GLN C C 176.485 0.040 1 133 40 40 GLN CA C 56.695 0.040 1 134 40 40 GLN N N 117.279 0.040 1 135 41 41 GLN H H 7.888 0.005 1 136 41 41 GLN CA C 56.415 0.040 1 137 41 41 GLN N N 120.869 0.040 1 138 44 44 ILE C C 176.235 0.040 1 139 45 45 PHE H H 8.748 0.005 1 140 45 45 PHE C C 174.795 0.040 1 141 45 45 PHE CA C 57.205 0.040 1 142 45 45 PHE N N 122.899 0.040 1 143 46 46 ALA H H 9.178 0.005 1 144 46 46 ALA C C 177.595 0.040 1 145 46 46 ALA CA C 52.425 0.040 1 146 46 46 ALA N N 135.359 0.040 1 147 47 47 GLY H H 8.618 0.005 1 148 47 47 GLY C C 173.725 0.040 1 149 47 47 GLY CA C 45.505 0.040 1 150 47 47 GLY N N 103.959 0.040 1 151 48 48 LYS H H 8.068 0.005 1 152 48 48 LYS C C 174.635 0.040 1 153 48 48 LYS CA C 54.305 0.040 1 154 48 48 LYS CB C 34.465 0.040 1 155 48 48 LYS N N 122.929 0.040 1 156 49 49 GLN H H 8.958 0.005 1 157 49 49 GLN C C 175.585 0.040 1 158 49 49 GLN CA C 55.945 0.040 1 159 49 49 GLN N N 123.199 0.040 1 160 50 50 LEU H H 8.518 0.005 1 161 50 50 LEU CA C 53.705 0.040 1 162 50 50 LEU N N 125.589 0.040 1 163 51 51 GLU C C 175.725 0.040 1 164 51 51 GLU CA C 56.575 0.040 1 165 52 52 ASP H H 8.678 0.005 1 166 52 52 ASP CA C 55.425 0.040 1 167 52 52 ASP N N 121.749 0.040 1 168 53 53 GLY C C 174.985 0.040 1 169 53 53 GLY CA C 45.325 0.040 1 170 54 54 ARG H H 7.738 0.005 1 171 54 54 ARG C C 175.215 0.040 1 172 54 54 ARG CA C 54.335 0.040 1 173 54 54 ARG N N 121.019 0.040 1 174 55 55 THR H H 9.018 0.005 1 175 55 55 THR C C 176.465 0.040 1 176 55 55 THR CA C 59.425 0.040 1 177 55 55 THR N N 109.079 0.040 1 178 56 56 LEU H H 8.318 0.005 1 179 56 56 LEU C C 180.955 0.040 1 180 56 56 LEU CA C 58.455 0.040 1 181 56 56 LEU N N 118.289 0.040 1 182 57 57 SER H H 8.938 0.005 1 183 57 57 SER C C 178.675 0.040 1 184 57 57 SER CA C 60.765 0.040 1 185 57 57 SER N N 114.529 0.040 1 186 58 58 ASP H H 8.118 0.005 1 187 58 58 ASP C C 177.275 0.040 1 188 58 58 ASP CA C 57.055 0.040 1 189 58 58 ASP N N 125.279 0.040 1 190 59 59 TYR H H 7.418 0.005 1 191 59 59 TYR C C 174.815 0.040 1 192 59 59 TYR CA C 58.095 0.040 1 193 59 59 TYR N N 116.899 0.040 1 194 60 60 ASN H H 8.258 0.005 1 195 60 60 ASN CA C 53.965 0.040 1 196 60 60 ASN N N 116.969 0.040 1 197 61 61 ILE C C 174.355 0.040 1 198 61 61 ILE CA C 62.305 0.040 1 199 62 62 GLN H H 8.078 0.005 1 200 62 62 GLN C C 175.295 0.040 1 201 62 62 GLN CA C 53.545 0.040 1 202 62 62 GLN CB C 31.835 0.040 1 203 62 62 GLN N N 126.059 0.040 1 204 63 63 LYS H H 8.878 0.005 1 205 63 63 LYS C C 176.785 0.040 1 206 63 63 LYS CA C 57.495 0.040 1 207 63 63 LYS CB C 32.495 0.040 1 208 63 63 LYS N N 120.929 0.040 1 209 64 64 GLU H H 9.678 0.005 1 210 64 64 GLU C C 175.335 0.040 1 211 64 64 GLU CA C 57.795 0.040 1 212 64 64 GLU N N 115.739 0.040 1 213 65 65 SER H H 7.988 0.005 1 214 65 65 SER C C 172.115 0.040 1 215 65 65 SER CA C 60.925 0.040 1 216 65 65 SER CB C 64.995 0.040 1 217 65 65 SER N N 116.799 0.040 1 218 66 66 THR H H 9.218 0.005 1 219 66 66 THR C C 173.795 0.040 1 220 66 66 THR CA C 62.155 0.040 1 221 66 66 THR N N 117.569 0.040 1 222 67 67 LEU H H 9.558 0.005 1 223 67 67 LEU C C 175.745 0.040 1 224 67 67 LEU CA C 53.905 0.040 1 225 67 67 LEU N N 128.739 0.040 1 226 68 68 HIS H H 9.198 0.005 1 227 68 68 HIS C C 173.745 0.040 1 228 68 68 HIS CA C 55.145 0.040 1 229 68 68 HIS N N 116.689 0.040 1 230 69 69 LEU H H 8.408 0.005 1 231 69 69 LEU C C 176.525 0.040 1 232 69 69 LEU CA C 54.235 0.040 1 233 69 69 LEU N N 124.369 0.040 1 234 70 70 VAL H H 8.888 0.005 1 235 70 70 VAL CA C 59.535 0.040 1 236 70 70 VAL N N 117.569 0.040 1 237 71 71 LEU H H 8.758 0.005 1 238 71 71 LEU C C 175.275 0.040 1 239 71 71 LEU CA C 55.405 0.040 1 240 71 71 LEU N N 123.919 0.040 1 241 72 72 ARG H H 8.478 0.005 1 242 72 72 ARG C C 175.895 0.040 1 243 72 72 ARG CA C 56.115 0.040 1 244 72 72 ARG CB C 30.705 0.040 1 245 72 72 ARG N N 123.859 0.040 1 246 73 73 LEU H H 8.798 0.005 1 247 73 73 LEU C C 177.515 0.040 1 248 73 73 LEU CA C 54.745 0.040 1 249 73 73 LEU CB C 41.985 0.040 1 250 73 73 LEU N N 126.139 0.040 1 251 74 74 ARG H H 8.928 0.005 1 252 74 74 ARG C C 177.245 0.040 1 253 74 74 ARG CA C 56.555 0.040 1 254 74 74 ARG CB C 30.355 0.040 1 255 74 74 ARG N N 123.899 0.040 1 256 75 75 GLY H H 8.848 0.005 1 257 75 75 GLY C C 173.965 0.040 1 258 75 75 GLY CA C 45.355 0.040 1 259 75 75 GLY N N 112.369 0.040 1 260 76 76 GLY H H 8.338 0.005 1 261 76 76 GLY CA C 46.145 0.040 1 262 76 76 GLY N N 115.919 0.040 1 stop_ save_ save_assigned_chem_shift_list_2 _Saveframe_category assigned_chemical_shifts _Details 'Denatured state of ubiquitin at 258K and 2500 bar' loop_ _Experiment_label '2D 1H-15N HSQC' '3D HNCO' '3D HNCA' '3D CBCA(CO)NH' '3D 1H-15N NOESY' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name Ubiquitin_denatured _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 1 1 MET C C 172.285 0.040 1 2 1 1 MET CA C 54.675 0.040 1 3 1 1 MET CB C 32.565 0.040 1 4 2 2 GLN H H 9.108 0.005 1 5 2 2 GLN C C 175.355 0.040 1 6 2 2 GLN CA C 55.515 0.040 1 7 2 2 GLN CB C 29.545 0.040 1 8 2 2 GLN N N 124.939 0.040 1 9 3 3 ILE H H 8.818 0.005 1 10 3 3 ILE C C 175.835 0.040 1 11 3 3 ILE CA C 60.695 0.040 1 12 3 3 ILE CB C 38.555 0.040 1 13 3 3 ILE N N 125.429 0.040 1 14 4 4 PHE H H 8.978 0.005 1 15 4 4 PHE C C 175.345 0.040 1 16 4 4 PHE CA C 57.825 0.040 1 17 4 4 PHE CB C 39.755 0.040 1 18 4 4 PHE N N 127.339 0.040 1 19 5 5 VAL H H 8.518 0.005 1 20 5 5 VAL C C 175.195 0.040 1 21 5 5 VAL CA C 61.535 0.040 1 22 5 5 VAL CB C 33.095 0.040 1 23 5 5 VAL N N 126.589 0.040 1 24 6 6 LYS H H 8.758 0.005 1 25 6 6 LYS C C 176.915 0.040 1 26 6 6 LYS CA C 56.375 0.040 1 27 6 6 LYS CB C 33.015 0.040 1 28 6 6 LYS N N 127.559 0.040 1 29 7 7 THR H H 8.778 0.005 1 30 7 7 THR C C 174.585 0.040 1 31 7 7 THR CA C 61.935 0.040 1 32 7 7 THR CB C 69.825 0.040 1 33 7 7 THR N N 119.209 0.040 1 34 8 8 LEU H H 8.928 0.005 1 35 8 8 LEU C C 177.865 0.040 1 36 8 8 LEU CA C 55.235 0.040 1 37 8 8 LEU CB C 41.965 0.040 1 38 8 8 LEU N N 126.589 0.040 1 39 9 9 THR H H 8.598 0.005 1 40 9 9 THR C C 175.235 0.040 1 41 9 9 THR CA C 61.625 0.040 1 42 9 9 THR CB C 69.815 0.040 1 43 9 9 THR N N 115.389 0.040 1 44 10 10 GLY H H 8.668 0.005 1 45 10 10 GLY C C 174.075 0.040 1 46 10 10 GLY CA C 45.315 0.040 1 47 10 10 GLY N N 112.439 0.040 1 48 11 11 LYS H H 8.508 0.005 1 49 11 11 LYS C C 177.065 0.040 1 50 11 11 LYS CA C 55.935 0.040 1 51 11 11 LYS CB C 33.135 0.040 1 52 11 11 LYS N N 122.029 0.040 1 53 12 12 THR H H 8.808 0.005 1 54 12 12 THR C C 174.505 0.040 1 55 12 12 THR CA C 62.285 0.040 1 56 12 12 THR CB C 69.615 0.040 1 57 12 12 THR N N 119.129 0.040 1 58 13 13 ILE H H 8.838 0.005 1 59 13 13 ILE C C 176.365 0.040 1 60 13 13 ILE CA C 60.735 0.040 1 61 13 13 ILE CB C 38.605 0.040 1 62 13 13 ILE N N 126.459 0.040 1 63 14 14 THR H H 8.858 0.005 1 64 14 14 THR C C 174.145 0.040 1 65 14 14 THR CA C 61.805 0.040 1 66 14 14 THR CB C 69.545 0.040 1 67 14 14 THR N N 122.519 0.040 1 68 15 15 LEU H H 8.838 0.005 1 69 15 15 LEU C C 177.145 0.040 1 70 15 15 LEU CA C 54.785 0.040 1 71 15 15 LEU CB C 42.225 0.040 1 72 15 15 LEU N N 127.439 0.040 1 73 16 16 GLU H H 8.838 0.005 1 74 16 16 GLU C C 176.315 0.040 1 75 16 16 GLU CA C 56.085 0.040 1 76 16 16 GLU CB C 29.995 0.040 1 77 16 16 GLU N N 124.229 0.040 1 78 17 17 VAL H H 8.688 0.005 1 79 17 17 VAL C C 176.105 0.040 1 80 17 17 VAL CA C 61.645 0.040 1 81 17 17 VAL CB C 32.975 0.040 1 82 17 17 VAL N N 123.209 0.040 1 83 18 18 GLU H H 8.998 0.005 1 84 18 18 GLU CA C 54.185 0.040 1 85 18 18 GLU N N 127.799 0.040 1 86 19 19 PRO C C 177.115 0.040 1 87 19 19 PRO CA C 63.205 0.040 1 88 19 19 PRO CB C 32.275 0.040 1 89 20 20 SER H H 8.918 0.005 1 90 20 20 SER C C 174.605 0.040 1 91 20 20 SER CA C 58.305 0.040 1 92 20 20 SER CB C 63.845 0.040 1 93 20 20 SER N N 117.589 0.040 1 94 21 21 ASP H H 8.788 0.005 1 95 21 21 ASP C C 176.535 0.040 1 96 21 21 ASP CA C 54.205 0.040 1 97 21 21 ASP CB C 40.925 0.040 1 98 21 21 ASP N N 123.409 0.040 1 99 22 22 THR H H 8.538 0.005 1 100 22 22 THR C C 174.805 0.040 1 101 22 22 THR CA C 61.925 0.040 1 102 22 22 THR CB C 69.855 0.040 1 103 22 22 THR N N 115.869 0.040 1 104 23 23 ILE H H 8.638 0.005 1 105 23 23 ILE C C 176.905 0.040 1 106 23 23 ILE CA C 61.585 0.040 1 107 23 23 ILE CB C 40.125 0.040 1 108 23 23 ILE N N 124.639 0.040 1 109 24 24 GLU H H 8.858 0.005 1 110 24 24 GLU C C 176.735 0.040 1 111 24 24 GLU CA C 56.875 0.040 1 112 24 24 GLU CB C 29.885 0.040 1 113 24 24 GLU N N 125.339 0.040 1 114 25 25 ASN H H 8.828 0.005 1 115 25 25 ASN C C 175.835 0.040 1 116 25 25 ASN CA C 53.545 0.040 1 117 25 25 ASN CB C 38.495 0.040 1 118 25 25 ASN N N 121.719 0.040 1 119 26 26 VAL H H 8.538 0.005 1 120 26 26 VAL C C 176.955 0.040 1 121 26 26 VAL CA C 63.065 0.040 1 122 26 26 VAL N N 122.449 0.040 1 123 27 27 LYS H H 8.658 0.005 1 124 27 27 LYS C C 177.025 0.040 1 125 27 27 LYS CA C 56.685 0.040 1 126 27 27 LYS N N 125.759 0.040 1 127 28 28 ALA H H 8.608 0.005 1 128 28 28 ALA C C 178.165 0.040 1 129 28 28 ALA CA C 52.765 0.040 1 130 28 28 ALA CB C 19.365 0.040 1 131 28 28 ALA N N 125.979 0.040 1 132 29 29 LYS H H 8.568 0.005 1 133 29 29 LYS C C 177.275 0.040 1 134 29 29 LYS CA C 56.465 0.040 1 135 29 29 LYS CB C 32.895 0.040 1 136 29 29 LYS N N 122.219 0.040 1 137 30 30 ILE H H 8.588 0.005 1 138 30 30 ILE C C 176.685 0.040 1 139 30 30 ILE CA C 61.295 0.040 1 140 30 30 ILE N N 124.339 0.040 1 141 31 31 GLN H H 8.848 0.005 1 142 31 31 GLN C C 176.125 0.040 1 143 31 31 GLN CA C 55.835 0.040 1 144 31 31 GLN CB C 29.265 0.040 1 145 31 31 GLN N N 125.899 0.040 1 146 32 32 ASP H H 8.818 0.005 1 147 32 32 ASP C C 176.625 0.040 1 148 32 32 ASP CA C 54.675 0.040 1 149 32 32 ASP CB C 40.895 0.040 1 150 32 32 ASP N N 123.529 0.040 1 151 33 33 LYS H H 8.668 0.005 1 152 33 33 LYS C C 176.985 0.040 1 153 33 33 LYS CA C 56.465 0.040 1 154 33 33 LYS CB C 32.825 0.040 1 155 33 33 LYS N N 122.489 0.040 1 156 34 34 GLU H H 8.718 0.005 1 157 34 34 GLU C C 177.215 0.040 1 158 34 34 GLU CA C 56.505 0.040 1 159 34 34 GLU CB C 29.925 0.040 1 160 34 34 GLU N N 122.389 0.040 1 161 35 35 GLY H H 8.668 0.005 1 162 35 35 GLY C C 173.875 0.040 1 163 35 35 GLY CA C 45.245 0.040 1 164 35 35 GLY N N 111.119 0.040 1 165 36 36 ILE H H 8.428 0.005 1 166 36 36 ILE CA C 58.575 0.040 1 167 36 36 ILE N N 122.819 0.040 1 168 38 38 PRO C C 177.395 0.040 1 169 38 38 PRO CA C 63.835 0.040 1 170 38 38 PRO CB C 32.205 0.040 1 171 39 39 ASP H H 8.908 0.005 1 172 39 39 ASP C C 177.055 0.040 1 173 39 39 ASP CA C 54.885 0.040 1 174 39 39 ASP N N 119.739 0.040 1 175 40 40 GLN H H 8.438 0.005 1 176 40 40 GLN C C 177.035 0.040 1 177 40 40 GLN CA C 56.655 0.040 1 178 40 40 GLN N N 121.549 0.040 1 179 41 41 GLN H H 8.588 0.005 1 180 41 41 GLN CA C 57.015 0.040 1 181 41 41 GLN N N 121.589 0.040 1 182 45 45 PHE C C 174.105 0.040 9 183 46 46 ALA H H 8.798 0.005 9 184 46 46 ALA C C 178.585 0.040 9 185 46 46 ALA CA C 52.765 0.040 9 186 46 46 ALA CB C 19.345 0.040 9 187 46 46 ALA N N 129.829 0.040 9 188 47 47 GLY H H 8.028 0.005 1 189 47 47 GLY C C 174.575 0.040 1 190 47 47 GLY CA C 45.695 0.040 1 191 47 47 GLY N N 108.179 0.040 1 192 48 48 LYS H H 8.348 0.005 1 193 48 48 LYS CA C 56.555 0.040 1 194 48 48 LYS N N 122.179 0.040 1 195 49 49 GLN C C 176.775 0.040 1 196 50 50 LEU H H 8.608 0.005 1 197 50 50 LEU C C 177.895 0.040 1 198 50 50 LEU CA C 55.715 0.040 1 199 50 50 LEU CB C 41.745 0.040 1 200 50 50 LEU N N 124.259 0.040 1 201 51 51 GLU H H 8.648 0.005 1 202 51 51 GLU C C 176.845 0.040 1 203 51 51 GLU CA C 56.665 0.040 1 204 51 51 GLU N N 123.419 0.040 1 205 52 52 ASP H H 8.778 0.005 1 206 52 52 ASP C C 177.425 0.040 1 207 52 52 ASP CA C 54.755 0.040 1 208 52 52 ASP CB C 40.765 0.040 1 209 52 52 ASP N N 121.929 0.040 1 210 53 53 GLY H H 8.668 0.005 1 211 53 53 GLY C C 174.975 0.040 1 212 53 53 GLY CA C 45.905 0.040 1 213 53 53 GLY N N 110.219 0.040 1 214 54 54 ARG H H 8.218 0.005 1 215 54 54 ARG C C 177.025 0.040 1 216 54 54 ARG CA C 56.505 0.040 1 217 54 54 ARG N N 121.699 0.040 1 218 55 55 THR H H 8.738 0.005 1 219 55 55 THR C C 175.325 0.040 1 220 55 55 THR CA C 62.555 0.040 1 221 55 55 THR N N 116.839 0.040 1 222 56 56 LEU H H 8.708 0.005 1 223 56 56 LEU C C 178.055 0.040 1 224 56 56 LEU CA C 56.005 0.040 1 225 56 56 LEU N N 125.549 0.040 1 226 57 57 SER H H 8.678 0.005 1 227 57 57 SER CA C 59.125 0.040 1 228 57 57 SER N N 116.849 0.040 1 229 58 58 ASP C C 177.065 0.040 1 230 59 59 TYR H H 8.408 0.005 1 231 59 59 TYR C C 176.595 0.040 1 232 59 59 TYR CA C 59.295 0.040 1 233 59 59 TYR N N 121.779 0.040 1 234 60 60 ASN H H 8.588 0.005 1 235 60 60 ASN CA C 53.955 0.040 1 236 60 60 ASN N N 121.009 0.040 1 237 67 67 LEU C C 177.795 0.040 1 238 67 67 LEU CA C 56.075 0.040 1 239 67 67 LEU CB C 41.915 0.040 1 240 68 68 HIS H H 8.638 0.005 1 241 68 68 HIS C C 175.315 0.040 1 242 68 68 HIS CA C 56.155 0.040 1 243 68 68 HIS N N 119.509 0.040 1 244 69 69 LEU H H 8.308 0.005 1 245 69 69 LEU C C 175.745 0.040 9 246 69 69 LEU CA C 56.145 0.040 1 247 69 69 LEU N N 123.809 0.040 1 248 70 70 VAL H H 8.628 0.005 9 249 70 70 VAL CA C 63.435 0.040 9 250 70 70 VAL N N 117.829 0.040 9 251 74 74 ARG C C 177.155 0.040 1 252 74 74 ARG CA C 56.495 0.040 1 253 74 74 ARG CB C 30.355 0.040 1 254 75 75 GLY H H 8.608 0.005 1 255 75 75 GLY N N 111.159 0.040 1 stop_ save_