data_19277 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Structure of d[GGTTGGCGCGAAGCATTCGCGGGTTGG] duplex-quadruplex hybrid ; _BMRB_accession_number 19277 _BMRB_flat_file_name bmr19277.str _Entry_type original _Submission_date 2013-05-30 _Accession_date 2013-05-30 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Lim 'Kah Wai' . . 2 Phan 'Anh Tuan' . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 240 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2013-10-09 update BMRB 'update entry citation' 2013-07-08 original author 'original release' stop_ loop_ _Related_BMRB_accession_number _Relationship 19276 'd[CGCGAAGCATTCGCG] hairpin' 19278 'd[GCGCGAAGCATTCGCGGGGAGGTGGGGAAGGG] duplex-quadruplex hybrid' 19279 'd[GGGAAGGGCGCGAAGCATTCGCGAGGTAGG] duplex-quadruplex hybrid' 19280 'd[AGGGTGGGTGCTGGGGCGCGAAGCATTCGCGAGG] duplex-quadruplex hybrid' 19281 'd[TTGGGTGGGCGCGAAGCATTCGCGGGGTGGGT] duplex-quadruplex hybrid' stop_ save_ ############################# # Citation for this entry # ############################# save_Citation_1 _Saveframe_category entry_citation _Citation_full . _Citation_title 'Structural basis of DNA quadruplex-duplex junction formation.' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 23794476 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Lim 'Kah Wai' . . 2 Phan 'Anh Tuan' . . stop_ _Journal_abbreviation 'Angew. Chem. Int. Ed. Engl.' _Journal_name_full 'Angewandte Chemie (International ed. in English)' _Journal_volume 52 _Journal_issue 33 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 8566 _Page_last 8569 _Year 2013 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'd[GGTTGGCGCGAAGCATTCGCGGGTTGG] duplex-quadruplex hybrid' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'DNA (27-MER)' $DNA_(27-MER) stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_DNA_(27-MER) _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class DNA _Name_common DNA_(27-MER) _Molecular_mass 8445.473 _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 27 _Mol_residue_sequence ; GGTTGGCGCGAAGCATTCGC GGGTTGG ; loop_ _Residue_seq_code _Residue_label 1 DG 2 DG 3 DT 4 DT 5 DG 6 DG 7 DC 8 DG 9 DC 10 DG 11 DA 12 DA 13 DG 14 DC 15 DA 16 DT 17 DT 18 DC 19 DG 20 DC 21 DG 22 DG 23 DG 24 DT 25 DT 26 DG 27 DG stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $DNA_(27-MER) . . . . . . stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $DNA_(27-MER) 'chemical synthesis' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_DNA-1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $DNA_(27-MER) 0.5-2.0 mM 'natural abundance' H2O 90 % 'natural abundance' D2O 10 % 'natural abundance' stop_ save_ save_DNA-2 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $DNA_(27-MER) 0.5-2.0 mM 'natural abundance' D2O 100 % 'natural abundance' stop_ save_ save_DNA-3 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $DNA_(27-MER) 0.5-2.0 mM '[U-2% 15N]' H2O 90 % 'natural abundance' D2O 10 % 'natural abundance' stop_ save_ save_DNA-4 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $DNA_(27-MER) 0.5-2.0 mM '[U-100% 2H]' D2O 100 % 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_TOPSPIN _Saveframe_category software _Name TOPSPIN _Version 2.1 loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . stop_ loop_ _Task processing stop_ _Details . save_ save_Felix _Saveframe_category software _Name Felix _Version 2007 loop_ _Vendor _Address _Electronic_address 'Felix NMR, Inc.' . . stop_ loop_ _Task 'peak picking' stop_ _Details . save_ save_X-PLOR_NIH _Saveframe_category software _Name X-PLOR_NIH _Version 2.29 loop_ _Vendor _Address _Electronic_address 'Schwieters, Kuszewski, Tjandra and Clore' . . stop_ loop_ _Task refinement stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 400 _Details . save_ save_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 600 _Details . save_ save_spectrometer_3 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 700 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-1H_NOESY_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $DNA-2 save_ save_2D_1H-1H_JR_NOESY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H JR NOESY' _Sample_label $DNA-1 save_ save_2D_1H-1H_JR_NOESY_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H JR NOESY' _Sample_label $DNA-1 save_ save_2D_1H-1H_COSY_4 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H COSY' _Sample_label $DNA-2 save_ save_2D_1H-1H_TOCSY_5 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H TOCSY' _Sample_label $DNA-2 save_ save_2D_1H-13C_HSQC_6 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HSQC' _Sample_label $DNA-2 save_ save_2D_1H-13C_JR_HMBC_7 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C JR HMBC' _Sample_label $DNA-1 save_ save_2D_1H-31P_HSQC_8 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-31P HSQC' _Sample_label $DNA-2 save_ save_H-D_EXCHANGE_9 _Saveframe_category NMR_applied_experiment _Experiment_name 'H-D EXCHANGE' _Sample_label $DNA-2 save_ save_15N-FILTERED_10 _Saveframe_category NMR_applied_experiment _Experiment_name 15N-FILTERED _Sample_label $DNA-3 save_ save_D-LABELED_11 _Saveframe_category NMR_applied_experiment _Experiment_name D-LABELED _Sample_label $DNA-4 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units temperature 298 . K pH 7 . pH pressure 1 . atm 'ionic strength' 40 . mM stop_ save_ save_sample_conditions_2 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units temperature 278 . K pH 7 . pH pressure 1 . atm 'ionic strength' 40 . mM stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-1H NOESY' '2D 1H-1H JR NOESY' 15N-FILTERED D-LABELED stop_ loop_ _Sample_label $DNA-2 $DNA-1 $DNA-3 $DNA-4 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'DNA (27-MER)' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 1 1 DG H1 H 12.103 0.010 1 2 1 1 DG H1' H 6.051 0.010 1 3 1 1 DG H2' H 2.931 0.010 1 4 1 1 DG H2'' H 2.980 0.010 1 5 1 1 DG H3' H 5.047 0.010 1 6 1 1 DG H4' H 4.395 0.010 1 7 1 1 DG H5' H 3.960 0.010 2 8 1 1 DG H5'' H 3.960 0.010 2 9 1 1 DG H8 H 7.457 0.010 1 10 2 2 DG H1 H 12.103 0.010 1 11 2 2 DG H1' H 6.059 0.010 1 12 2 2 DG H2' H 3.069 0.010 1 13 2 2 DG H2'' H 2.408 0.010 1 14 2 2 DG H3' H 5.179 0.010 1 15 2 2 DG H4' H 4.454 0.010 1 16 2 2 DG H5' H 4.283 0.010 2 17 2 2 DG H5'' H 4.283 0.010 2 18 2 2 DG H8 H 8.281 0.010 1 19 3 3 DT H1' H 6.197 0.010 1 20 3 3 DT H2' H 2.214 0.010 1 21 3 3 DT H2'' H 2.585 0.010 1 22 3 3 DT H3' H 4.905 0.010 1 23 3 3 DT H4' H 4.288 0.010 1 24 3 3 DT H5' H 4.335 0.010 2 25 3 3 DT H5'' H 4.245 0.010 2 26 3 3 DT H6 H 7.888 0.010 1 27 3 3 DT H71 H 2.008 0.010 2 28 3 3 DT H72 H 2.008 0.010 2 29 3 3 DT H73 H 2.008 0.010 2 30 4 4 DT H1' H 6.001 0.010 1 31 4 4 DT H2' H 2.042 0.010 1 32 4 4 DT H2'' H 2.657 0.010 1 33 4 4 DT H3 H 10.768 0.010 1 34 4 4 DT H3' H 4.900 0.010 1 35 4 4 DT H4' H 4.277 0.010 1 36 4 4 DT H5' H 4.181 0.010 2 37 4 4 DT H5'' H 3.919 0.010 2 38 4 4 DT H6 H 7.074 0.010 1 39 4 4 DT H71 H 0.949 0.010 2 40 4 4 DT H72 H 0.949 0.010 2 41 4 4 DT H73 H 0.949 0.010 2 42 5 5 DG H1 H 11.892 0.010 1 43 5 5 DG H1' H 6.126 0.010 1 44 5 5 DG H2' H 3.461 0.010 1 45 5 5 DG H2'' H 3.052 0.010 1 46 5 5 DG H3' H 4.916 0.010 1 47 5 5 DG H4' H 4.464 0.010 1 48 5 5 DG H8 H 7.475 0.010 1 49 6 6 DG H1 H 11.922 0.010 1 50 6 6 DG H1' H 5.668 0.010 1 51 6 6 DG H2' H 2.715 0.010 1 52 6 6 DG H2'' H 2.478 0.010 1 53 6 6 DG H3' H 5.021 0.010 1 54 6 6 DG H4' H 4.444 0.010 1 55 6 6 DG H5' H 4.321 0.010 2 56 6 6 DG H5'' H 4.246 0.010 2 57 6 6 DG H8 H 8.099 0.010 1 58 7 7 DC H1' H 6.146 0.010 1 59 7 7 DC H2' H 1.963 0.010 1 60 7 7 DC H2'' H 2.499 0.010 1 61 7 7 DC H3' H 5.025 0.010 1 62 7 7 DC H4' H 4.346 0.010 1 63 7 7 DC H5 H 5.554 0.010 1 64 7 7 DC H6 H 7.547 0.010 1 65 7 7 DC H41 H 8.125 0.010 1 66 7 7 DC H42 H 6.262 0.010 1 67 8 8 DG H1 H 12.887 0.010 1 68 8 8 DG H1' H 5.795 0.010 1 69 8 8 DG H2' H 2.658 0.010 1 70 8 8 DG H2'' H 2.709 0.010 1 71 8 8 DG H3' H 5.000 0.010 1 72 8 8 DG H4' H 4.352 0.010 1 73 8 8 DG H8 H 7.937 0.010 1 74 9 9 DC H1' H 5.596 0.010 1 75 9 9 DC H2' H 1.833 0.010 1 76 9 9 DC H2'' H 2.289 0.010 1 77 9 9 DC H3' H 4.789 0.010 1 78 9 9 DC H4' H 4.136 0.010 1 79 9 9 DC H5 H 5.388 0.010 1 80 9 9 DC H6 H 7.256 0.010 1 81 9 9 DC H41 H 8.281 0.010 1 82 9 9 DC H42 H 6.379 0.010 1 83 10 10 DG H1 H 12.733 0.010 1 84 10 10 DG H1' H 5.414 0.010 1 85 10 10 DG H2' H 2.632 0.010 1 86 10 10 DG H2'' H 2.715 0.010 1 87 10 10 DG H3' H 4.974 0.010 1 88 10 10 DG H4' H 4.285 0.010 1 89 10 10 DG H8 H 7.863 0.010 1 90 11 11 DA H1' H 5.917 0.010 1 91 11 11 DA H2 H 7.599 0.010 1 92 11 11 DA H2' H 2.328 0.010 1 93 11 11 DA H2'' H 2.642 0.010 1 94 11 11 DA H3' H 4.985 0.010 1 95 11 11 DA H4' H 4.320 0.010 1 96 11 11 DA H8 H 7.928 0.010 1 97 12 12 DA H1' H 5.909 0.010 1 98 12 12 DA H2 H 7.728 0.010 1 99 12 12 DA H2' H 2.088 0.010 1 100 12 12 DA H2'' H 2.454 0.010 1 101 12 12 DA H3' H 4.910 0.010 1 102 12 12 DA H4' H 4.376 0.010 1 103 12 12 DA H8 H 7.480 0.010 1 104 13 13 DG H1' H 5.412 0.010 1 105 13 13 DG H2' H 2.663 0.010 1 106 13 13 DG H2'' H 2.373 0.010 1 107 13 13 DG H3' H 4.840 0.010 1 108 13 13 DG H4' H 4.404 0.010 1 109 13 13 DG H8 H 8.071 0.010 1 110 14 14 DC H1' H 5.730 0.010 1 111 14 14 DC H2' H 1.622 0.010 1 112 14 14 DC H2'' H 2.073 0.010 1 113 14 14 DC H3' H 4.443 0.010 1 114 14 14 DC H4' H 2.125 0.010 1 115 14 14 DC H5 H 5.237 0.010 1 116 14 14 DC H5' H 3.387 0.010 2 117 14 14 DC H5'' H 3.074 0.010 2 118 14 14 DC H6 H 7.224 0.010 1 119 15 15 DA H1' H 6.348 0.010 1 120 15 15 DA H2 H 8.064 0.010 1 121 15 15 DA H2' H 3.025 0.010 1 122 15 15 DA H2'' H 2.980 0.010 1 123 15 15 DA H3' H 4.846 0.010 1 124 15 15 DA H4' H 4.366 0.010 1 125 15 15 DA H5' H 4.008 0.010 2 126 15 15 DA H5'' H 3.867 0.010 2 127 15 15 DA H8 H 8.114 0.010 1 128 16 16 DT H1' H 5.710 0.010 1 129 16 16 DT H2' H 2.142 0.010 1 130 16 16 DT H2'' H 2.533 0.010 1 131 16 16 DT H3 H 13.303 0.010 1 132 16 16 DT H3' H 4.783 0.010 1 133 16 16 DT H4' H 4.324 0.010 1 134 16 16 DT H5' H 4.367 0.010 2 135 16 16 DT H5'' H 4.123 0.010 2 136 16 16 DT H6 H 7.439 0.010 1 137 16 16 DT H71 H 1.861 0.010 2 138 16 16 DT H72 H 1.861 0.010 2 139 16 16 DT H73 H 1.861 0.010 2 140 17 17 DT H1' H 6.080 0.010 1 141 17 17 DT H2' H 2.222 0.010 1 142 17 17 DT H2'' H 2.512 0.010 1 143 17 17 DT H3 H 13.891 0.010 1 144 17 17 DT H3' H 4.899 0.010 1 145 17 17 DT H4' H 4.254 0.010 1 146 17 17 DT H6 H 7.454 0.010 1 147 17 17 DT H71 H 1.653 0.010 2 148 17 17 DT H72 H 1.653 0.010 2 149 17 17 DT H73 H 1.653 0.010 2 150 18 18 DC H1' H 5.606 0.010 1 151 18 18 DC H2' H 2.064 0.010 1 152 18 18 DC H2'' H 2.356 0.010 1 153 18 18 DC H3' H 4.833 0.010 1 154 18 18 DC H4' H 4.141 0.010 1 155 18 18 DC H5 H 5.684 0.010 1 156 18 18 DC H6 H 7.501 0.010 1 157 18 18 DC H41 H 8.521 0.010 1 158 18 18 DC H42 H 6.902 0.010 1 159 19 19 DG H1 H 12.801 0.010 1 160 19 19 DG H1' H 5.737 0.010 1 161 19 19 DG H2' H 2.474 0.010 1 162 19 19 DG H2'' H 2.519 0.010 1 163 19 19 DG H3' H 4.862 0.010 1 164 19 19 DG H4' H 4.279 0.010 1 165 19 19 DG H5' H 4.052 0.010 2 166 19 19 DG H5'' H 3.986 0.010 2 167 19 19 DG H8 H 7.784 0.010 1 168 20 20 DC H1' H 5.564 0.010 1 169 20 20 DC H2' H 1.713 0.010 1 170 20 20 DC H2'' H 2.188 0.010 1 171 20 20 DC H3' H 4.608 0.010 1 172 20 20 DC H4' H 4.078 0.010 1 173 20 20 DC H5 H 5.174 0.010 1 174 20 20 DC H5' H 4.068 0.010 2 175 20 20 DC H5'' H 3.948 0.010 2 176 20 20 DC H6 H 7.138 0.010 1 177 20 20 DC H41 H 8.080 0.010 1 178 20 20 DC H42 H 6.213 0.010 1 179 21 21 DG H1 H 13.219 0.010 1 180 21 21 DG H1' H 5.872 0.010 1 181 21 21 DG H2' H 2.623 0.010 1 182 21 21 DG H2'' H 3.021 0.010 1 183 21 21 DG H3' H 5.000 0.010 1 184 21 21 DG H4' H 4.287 0.010 1 185 21 21 DG H5' H 4.048 0.010 2 186 21 21 DG H5'' H 3.944 0.010 2 187 21 21 DG H8 H 7.651 0.010 1 188 22 22 DG H1 H 11.296 0.010 1 189 22 22 DG H1' H 5.809 0.010 1 190 22 22 DG H2' H 3.110 0.010 1 191 22 22 DG H2'' H 2.686 0.010 1 192 22 22 DG H3' H 5.010 0.010 1 193 22 22 DG H4' H 4.347 0.010 1 194 22 22 DG H5' H 4.222 0.010 2 195 22 22 DG H5'' H 4.222 0.010 2 196 22 22 DG H8 H 7.326 0.010 1 197 23 23 DG H1 H 11.780 0.010 1 198 23 23 DG H1' H 5.952 0.010 1 199 23 23 DG H2' H 2.946 0.010 1 200 23 23 DG H2'' H 2.329 0.010 1 201 23 23 DG H3' H 5.100 0.010 1 202 23 23 DG H4' H 4.381 0.010 1 203 23 23 DG H8 H 8.183 0.010 1 204 24 24 DT H1' H 6.178 0.010 1 205 24 24 DT H2' H 2.151 0.010 1 206 24 24 DT H2'' H 2.531 0.010 1 207 24 24 DT H3' H 4.865 0.010 1 208 24 24 DT H4' H 4.273 0.010 1 209 24 24 DT H5' H 4.290 0.010 2 210 24 24 DT H5'' H 4.205 0.010 2 211 24 24 DT H6 H 7.818 0.010 1 212 24 24 DT H71 H 1.952 0.010 2 213 24 24 DT H72 H 1.952 0.010 2 214 24 24 DT H73 H 1.952 0.010 2 215 25 25 DT H1' H 6.016 0.010 1 216 25 25 DT H2' H 2.043 0.010 1 217 25 25 DT H2'' H 2.614 0.010 1 218 25 25 DT H3 H 10.784 0.010 1 219 25 25 DT H3' H 4.883 0.010 1 220 25 25 DT H4' H 4.266 0.010 1 221 25 25 DT H5' H 4.144 0.010 2 222 25 25 DT H5'' H 3.897 0.010 2 223 25 25 DT H6 H 7.078 0.010 1 224 25 25 DT H71 H 0.890 0.010 2 225 25 25 DT H72 H 0.890 0.010 2 226 25 25 DT H73 H 0.890 0.010 2 227 26 26 DG H1 H 11.671 0.010 1 228 26 26 DG H1' H 6.133 0.010 1 229 26 26 DG H2' H 3.576 0.010 1 230 26 26 DG H2'' H 3.069 0.010 1 231 26 26 DG H3' H 4.970 0.010 1 232 26 26 DG H4' H 4.447 0.010 1 233 26 26 DG H8 H 7.541 0.010 1 234 27 27 DG H1 H 11.945 0.010 1 235 27 27 DG H1' H 6.178 0.010 1 236 27 27 DG H2' H 2.699 0.010 1 237 27 27 DG H2'' H 2.449 0.010 1 238 27 27 DG H3' H 4.811 0.010 1 239 27 27 DG H4' H 4.247 0.010 1 240 27 27 DG H8 H 8.153 0.010 1 stop_ save_