data_19412
#######################
# Entry information #
#######################
save_entry_information
_Saveframe_category entry_information
_Entry_title
;
K11-linked Diubiquitin average solution structure at pH 6.8, 150 mM NaCl
;
_BMRB_accession_number 19412
_BMRB_flat_file_name bmr19412.str
_Entry_type original
_Submission_date 2013-08-03
_Accession_date 2013-08-03
_Entry_origination author
_NMR_STAR_version 2.1.1
_Experimental_method NMR
_Details .
loop_
_Author_ordinal
_Author_family_name
_Author_given_name
_Author_middle_initials
_Author_family_title
1 Castaneda Carlos A. .
2 Fushman David . .
stop_
loop_
_Saveframe_category_type
_Saveframe_category_type_count
assigned_chemical_shifts 2
stop_
loop_
_Data_type
_Data_type_count
"1H chemical shifts" 138
"15N chemical shifts" 138
stop_
loop_
_Revision_date
_Revision_keyword
_Revision_author
_Revision_detail
2013-09-03 original author .
stop_
loop_
_Related_BMRB_accession_number
_Relationship
19406 'K11-linked Ub2 average solution structure in the absence of NaCl'
stop_
_Original_release_date 2013-09-03
save_
#############################
# Citation for this entry #
#############################
save_entry_citation
_Saveframe_category entry_citation
_Citation_full .
_Citation_title 'Unique structural, dynamical, and functional properties of K11-linked polyubiquitin chains'
_Citation_status published
_Citation_type journal
_CAS_abstract_code .
_MEDLINE_UI_code .
_PubMed_ID 23823328
loop_
_Author_ordinal
_Author_family_name
_Author_given_name
_Author_middle_initials
_Author_family_title
1 Castaneda Carlos A. .
2 Kashyap Tanuja R. .
3 Nakasone Mark A. .
4 Krueger Susan . .
5 Fushman David . .
stop_
_Journal_abbreviation Structure
_Journal_volume 21
_Journal_issue 7
_Journal_CSD .
_Book_chapter_title .
_Book_volume .
_Book_series .
_Book_ISBN .
_Conference_state_province .
_Conference_abstract_number .
_Page_first 1168
_Page_last 1181
_Year 2013
_Details .
save_
##################################
# Molecular system description #
##################################
save_assembly
_Saveframe_category molecular_system
_Mol_system_name 'K11-linked Diubiquitin'
_Enzyme_commission_number .
loop_
_Mol_system_component_name
_Mol_label
'K11-linked Diubiquitin_1' $entity
'K11-linked Diubiquitin_2' $entity
stop_
_System_molecular_weight .
_System_physical_state native
_System_oligomer_state ?
_System_paramagnetic no
_System_thiol_state .
_Database_query_date .
_Details .
save_
########################
# Monomeric polymers #
########################
save_entity
_Saveframe_category monomeric_polymer
_Mol_type polymer
_Mol_polymer_class protein
_Name_common entity
_Molecular_mass 8576.914
_Mol_thiol_state 'not present'
_Details .
##############################
# Polymer residue sequence #
##############################
_Residue_count 152
_Mol_residue_sequence
;
MQIFVKTLTGKTITLEVEPS
DTIENVKAKIQDKEGIPPDQ
QRLIFAGKQLEDGRTLSDYN
IQKESTLHLVLRLRGGMQIF
VKTLTGKTITLEVEPSDTIE
NVKAKIQDKEGIPPDQQRLI
FAGKQLEDGRTLSDYNIQKE
STLHLVLRLRGG
;
loop_
_Residue_seq_code
_Residue_label
1 MET 2 GLN 3 ILE 4 PHE 5 VAL
6 LYS 7 THR 8 LEU 9 THR 10 GLY
11 LYS 12 THR 13 ILE 14 THR 15 LEU
16 GLU 17 VAL 18 GLU 19 PRO 20 SER
21 ASP 22 THR 23 ILE 24 GLU 25 ASN
26 VAL 27 LYS 28 ALA 29 LYS 30 ILE
31 GLN 32 ASP 33 LYS 34 GLU 35 GLY
36 ILE 37 PRO 38 PRO 39 ASP 40 GLN
41 GLN 42 ARG 43 LEU 44 ILE 45 PHE
46 ALA 47 GLY 48 LYS 49 GLN 50 LEU
51 GLU 52 ASP 53 GLY 54 ARG 55 THR
56 LEU 57 SER 58 ASP 59 TYR 60 ASN
61 ILE 62 GLN 63 LYS 64 GLU 65 SER
66 THR 67 LEU 68 HIS 69 LEU 70 VAL
71 LEU 72 ARG 73 LEU 74 ARG 75 GLY
76 GLY 77 MET 78 GLN 79 ILE 80 PHE
81 VAL 82 LYS 83 THR 84 LEU 85 THR
86 GLY 87 LYS 88 THR 89 ILE 90 THR
91 LEU 92 GLU 93 VAL 94 GLU 95 PRO
96 SER 97 ASP 98 THR 99 ILE 100 GLU
101 ASN 102 VAL 103 LYS 104 ALA 105 LYS
106 ILE 107 GLN 108 ASP 109 LYS 110 GLU
111 GLY 112 ILE 113 PRO 114 PRO 115 ASP
116 GLN 117 GLN 118 ARG 119 LEU 120 ILE
121 PHE 122 ALA 123 GLY 124 LYS 125 GLN
126 LEU 127 GLU 128 ASP 129 GLY 130 ARG
131 THR 132 LEU 133 SER 134 ASP 135 TYR
136 ASN 137 ILE 138 GLN 139 LYS 140 GLU
141 SER 142 THR 143 LEU 144 HIS 145 LEU
146 VAL 147 LEU 148 ARG 149 LEU 150 ARG
151 GLY 152 GLY
stop_
_Sequence_homology_query_date .
_Sequence_homology_query_revised_last_date 2015-11-25
loop_
_Database_name
_Database_accession_code
_Database_entry_mol_name
_Sequence_query_to_submitted_percentage
_Sequence_subject_length
_Sequence_identity
_Sequence_positive
_Sequence_homology_expectation_value
BMRB 11505 entity 50.00 76 98.68 98.68 6.39e-44
BMRB 11547 ubiquitin 50.00 76 98.68 98.68 6.39e-44
BMRB 15047 denatured_ubiquitin 50.00 76 100.00 100.00 1.29e-44
BMRB 15410 Ubi 50.00 76 100.00 100.00 1.29e-44
BMRB 15907 Ubiquitin 50.00 76 100.00 100.00 1.29e-44
BMRB 16228 ubiquitin 50.00 76 97.37 98.68 2.79e-43
BMRB 16582 Ubiquitin 50.00 76 100.00 100.00 1.29e-44
BMRB 16626 Ubiquitin 50.00 76 100.00 100.00 1.29e-44
BMRB 17181 ubiquitin 50.00 76 100.00 100.00 1.29e-44
BMRB 17439 ubiquitin 50.00 76 100.00 100.00 1.29e-44
BMRB 17769 Ubiquitin 50.00 76 100.00 100.00 1.29e-44
BMRB 17919 entity 50.00 76 100.00 100.00 1.29e-44
BMRB 18582 ubiquitin 50.00 76 100.00 100.00 1.29e-44
BMRB 18583 ubiquitin 50.00 76 100.00 100.00 1.29e-44
BMRB 18584 ubiquitin 50.00 76 100.00 100.00 1.29e-44
BMRB 18610 Ubiquitin 50.00 76 100.00 100.00 1.29e-44
BMRB 18611 Ubiquitin_A_state 50.00 76 100.00 100.00 1.29e-44
BMRB 18737 UBIQUITIN 50.00 76 100.00 100.00 1.29e-44
BMRB 19406 entity 100.00 152 100.00 100.00 2.92e-102
BMRB 25070 Ubiquitin 50.00 79 100.00 100.00 1.32e-44
BMRB 25601 entity_1 50.00 76 97.37 97.37 6.94e-43
BMRB 26604 Ubiquitin_(microcrystalline) 50.00 76 100.00 100.00 1.29e-44
BMRB 4245 ubiquitin 50.00 76 100.00 100.00 1.29e-44
BMRB 4375 Ubiquitin 50.00 76 100.00 100.00 1.29e-44
PDB 1AAR "Structure Of A Diubiquitin Conjugate And A Model For Interaction With Ubiquitin Conjugating Enzyme (E2)" 50.00 76 100.00 100.00 1.29e-44
PDB 1D3Z "Ubiquitin Nmr Structure" 50.00 76 100.00 100.00 1.29e-44
PDB 1F9J "Structure Of A New Crystal Form Of Tetraubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 1FXT "Structure Of A Conjugating Enzyme-Ubiquitin Thiolester Complex" 50.00 76 100.00 100.00 1.29e-44
PDB 1OGW "Synthetic Ubiquitin With Fluoro-Leu At 50 And 67" 50.00 76 97.37 97.37 4.22e-43
PDB 1P3Q "Mechanism Of Ubiquitin Recognition By The Cue Domain Of Vps9" 50.00 76 100.00 100.00 1.29e-44
PDB 1Q5W "Ubiquitin Recognition By Npl4 Zinc-Fingers" 50.00 76 100.00 100.00 1.29e-44
PDB 1TBE "Structure Of Tetraubiquitin Shows How Multiubiquitin Chains Can Be Formed" 50.00 76 100.00 100.00 1.29e-44
PDB 1UBI "Synthetic Structural And Biological Studies Of The Ubiquitin System. Part 1" 50.00 76 100.00 100.00 1.29e-44
PDB 1UBQ "Structure Of Ubiquitin Refined At 1.8 Angstroms Resolution" 50.00 76 100.00 100.00 1.29e-44
PDB 1UZX "A Complex Of The Vps23 Uev With Ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 1V80 "Solution Structures Of Ubiquitin At 30 Bar And 3 Kbar" 50.00 76 100.00 100.00 1.29e-44
PDB 1V81 "Solution Structures Of Ubiquitin At 30 Bar And 3 Kbar" 50.00 76 100.00 100.00 1.29e-44
PDB 1VX7 "Cryo-em Structure Of The Plasmodium Falciparum 80s Ribosome Bound To The Anti-protozoan Drug Emetine, Large Subunit (protein On" 50.00 128 98.68 100.00 1.96e-44
PDB 1WR6 "Crystal Structure Of Gga3 Gat Domain In Complex With Ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 1WRD "Crystal Structure Of Tom1 Gat Domain In Complex With Ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 1XQQ "Simultaneous Determination Of Protein Structure And Dynamics" 50.00 76 100.00 100.00 1.29e-44
PDB 1YD8 "Complex Of Human Gga3 Gat Domain And Ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 1YIW "X-Ray Crystal Structure Of A Chemically Synthesized Ubiquitin" 50.00 76 98.68 100.00 5.74e-44
PDB 1YJ1 "X-Ray Crystal Structure Of A Chemically Synthesized [d-Gln35]ubiquitin" 50.00 76 97.37 98.68 6.50e-43
PDB 1YX5 "Solution Structure Of S5a Uim-1UBIQUITIN COMPLEX" 50.00 98 100.00 100.00 4.10e-45
PDB 1YX6 "Solution Structure Of S5a Uim-2UBIQUITIN COMPLEX" 50.00 98 100.00 100.00 4.10e-45
PDB 1ZGU "Solution Structure Of The Human Mms2-Ubiquitin Complex" 50.00 76 98.68 100.00 2.90e-44
PDB 2BGF "Nmr Structure Of Lys48-Linked Di-Ubiquitin Using Chemical Shift Perturbation Data Together With Rdcs And 15n- Relaxation Data" 50.00 76 100.00 100.00 1.29e-44
PDB 2C7M "Human Rabex-5 Residues 1-74 In Complex With Ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 2C7N "Human Rabex-5 Residues 1-74 In Complex With Ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 2D3G "Double Sided Ubiquitin Binding Of Hrs-Uim" 50.00 76 100.00 100.00 1.29e-44
PDB 2DEN "Solution Structure Of The Ubiquitin-Associated Domain Of Human Bmsc-Ubp And Its Complex With Ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 2DX5 "The Complex Structure Between The Mouse Eap45-Glue Domain And Ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 2FCM "X-Ray Crystal Structure Of A Chemically Synthesized [d-Gln35]ubiquitin With A Cubic Space Group" 50.00 76 97.37 98.68 6.50e-43
PDB 2FCN "X-Ray Crystal Structure Of A Chemically Synthesized [d-Val35]ubiquitin With A Cubic Space Group" 50.00 76 97.37 98.68 6.50e-43
PDB 2FCQ "X-Ray Crystal Structure Of A Chemically Synthesized Ubiquitin With A Cubic Space Group" 50.00 76 98.68 100.00 5.74e-44
PDB 2FCS "X-Ray Crystal Structure Of A Chemically Synthesized [l-Gln35]ubiquitin With A Cubic Space Group" 50.00 76 97.37 98.68 6.30e-43
PDB 2FID "Crystal Structure Of A Bovine Rabex-5 Fragment Complexed With Ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 2FIF "Crystal Structure Of A Bovine Rabex-5 Fragment Complexed With Ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 2FUH "Solution Structure Of The Ubch5cUB NON-Covalent Complex" 50.00 76 100.00 100.00 1.29e-44
PDB 2G45 "Co-Crystal Structure Of Znf Ubp Domain From The Deubiquitinating Enzyme Isopeptidase T (Isot) In Complex With Ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 2GMI Mms2UBC13~UBIQUITIN 50.00 76 100.00 100.00 1.29e-44
PDB 2HD5 "Usp2 In Complex With Ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 2HTH "Structural Basis For Ubiquitin Recognition By The Human Eap45ESCRT-Ii Glue Domain" 50.00 76 100.00 100.00 1.29e-44
PDB 2JF5 "Crystal Structure Of Lys63-Linked Di-Ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 2JRI "Solution Structure Of The Josephin Domain Of Ataxin-3 In Complex With Ubiquitin Molecule." 50.00 76 100.00 100.00 1.29e-44
PDB 2JY6 "Solution Structure Of The Complex Of Ubiquitin And Ubiquilin 1 Uba Domain" 50.00 76 100.00 100.00 1.29e-44
PDB 2JZZ "Solid-State Nmr Structure Of Microcrystalline Ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 2K39 "Recognition Dynamics Up To Microseconds Revealed From Rdc Derived Ubiquitin Ensemble In Solution" 50.00 76 100.00 100.00 1.29e-44
PDB 2K8B "Solution Structure Of Plaa Family Ubiquitin Binding Domain (Pfuc) Cis Isomer In Complex With Ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 2K8C "Solution Structure Of Plaa Family Ubiquitin Binding Domain (Pfuc) Trans Isomer In Complex With Ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 2KDE "Nmr Structure Of Major S5a (196-306):k48 Linked Diubiquitin Species" 50.00 76 100.00 100.00 1.29e-44
PDB 2KDF "Nmr Structure Of Minor S5a (196-306):k48 Linked Diubiquitin Species" 50.00 76 100.00 100.00 1.29e-44
PDB 2KHW "Solution Structure Of The Human Polymerase Iota Ubm2- Ubiquitin Complex" 50.00 79 100.00 100.00 1.32e-44
PDB 2KLG "Pere Nmr Structure Of Ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 2KN5 "A Correspondence Between Solution-State Dynamics Of An Individual Protein And The Sequence And Conformational Diversity Of Its " 50.00 76 100.00 100.00 1.29e-44
PDB 2KOX "Nmr Residual Dipolar Couplings Identify Long Range Correlated Motions In The Backbone Of The Protein Ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 2KTF "Solution Nmr Structure Of Human Polymerase Iota Ubm2 In Complex With Ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 2KWU "Solution Structure Of Ubm2 Of Murine Polymerase Iota In Complex With Ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 2KWV "Solution Structure Of Ubm1 Of Murine Polymerase Iota In Complex With Ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 2L0F "Solution Nmr Structure Of Human Polymerase Iota Ubm2 (P692a Mutant) In Complex With Ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 2L0T "Solution Structure Of The Complex Of Ubiquitin And The Vhs Domain Of Stam2" 50.00 76 100.00 100.00 1.29e-44
PDB 2L3Z "Proton-Detected 4d Dream Solid-State Nmr Structure Of Ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 2LD9 "Backbone Structure Of Ubiquitin Determined Using Backbone Amide Noes And Backbone N-H And N-C Rdcs" 50.00 77 100.00 100.00 1.54e-44
PDB 2LJ5 "Description Of The Structural Fluctuations Of Proteins From Structure- Based Calculations Of Residual Dipolar Couplings" 50.00 76 100.00 100.00 1.29e-44
PDB 2LVO "Structure Of The Gp78cue Domain Bound To Monubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 2LVP "Gp78cue Domain Bound To The Distal Ubiquitin Of K48-Linked Diubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 2LVQ "Gp78cue Domain Bound To The Proximal Ubiquitin Of K48-Linked Diubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 2LZ6 "Distinct Ubiquitin Binding Modes Exhibited By Sh3 Domains: Molecular Determinants And Functional Implications" 50.00 76 100.00 100.00 1.29e-44
PDB 2MBB "Solution Structure Of The Human Polymerase Iota Ubm1-ubiquitin Complex" 50.00 78 100.00 100.00 1.00e-44
PDB 2MBH "Nmr Structure Of Eklf(22-40)/ubiquitin Complex" 50.00 76 100.00 100.00 1.29e-44
PDB 2MBO "K11-linked Diubiquitin Average Solution Structure At Ph 6.8, 0 Mm Nacl" 50.00 76 100.00 100.00 1.29e-44
PDB 2MBQ "K11-linked Diubiquitin Average Solution Structure At Ph 6.8, 150 Mm Nacl" 50.00 76 100.00 100.00 1.29e-44
PDB 2MCN "Distinct Ubiquitin Binding Modes Exhibited By Sh3 Domains: Molecular Determinants And Functional Implications" 50.00 76 100.00 100.00 1.29e-44
PDB 2MJ5 "Structure Of The Uba Domain Of Human Nbr1 In Complex With Ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 2MJB "Solution Nmr Structure Of Ubiquitin Refined Against Dipolar Couplings In 4 Media" 50.00 76 100.00 100.00 1.29e-44
PDB 2MOR "A Tensor-free Method For The Structural And Dynamical Refinement Of Proteins Using Residual Dipolar Couplings" 50.00 76 100.00 100.00 1.29e-44
PDB 2MRE "Nmr Structure Of The Rad18-ubz/ubiquitin Complex" 50.00 79 100.00 100.00 1.32e-44
PDB 2MRO "Structure Of The Complex Of Ubiquitin And The Uba Domain From Dna- Damage-inducible 1 Protein (ddi1)" 50.00 76 100.00 100.00 1.29e-44
PDB 2MUR "Solution Structure Of The Human Faap20 Ubz-ubiquitin Complex" 50.00 78 100.00 100.00 1.00e-44
PDB 2MWS "Structure Of The Complex Of Ubiquitin And The Ubiquitin-like (ubl) Domain Of Ddi1" 50.00 76 98.68 98.68 7.28e-44
PDB 2N2K "Ensemble Structure Of The Closed State Of Lys63-linked Diubiquitin In The Absence Of A Ligand" 50.00 76 97.37 97.37 6.94e-43
PDB 2NR2 "The Mumo (Minimal Under-Restraining Minimal Over- Restraining) Method For The Determination Of Native States Ensembles Of Prote" 50.00 76 100.00 100.00 1.29e-44
PDB 2O6V "Crystal Structure And Solution Nmr Studies Of Lys48-Linked Tetraubiquitin At Neutral Ph" 50.00 76 100.00 100.00 1.29e-44
PDB 2OJR "Structure Of Ubiquitin Solved By Sad Using The Lanthanide- Binding Tag" 50.00 111 100.00 100.00 4.40e-44
PDB 2OOB "Crystal Structure Of The Uba Domain From Cbl-B Ubiquitin Ligase In Complex With Ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 2PE9 "Nmr Based Structure Of The Open Conformation Of Lys48- Linked Di-Ubiquitin Using Experimental Global Rotational Diffusion Tenso" 50.00 76 100.00 100.00 1.29e-44
PDB 2PEA "Nmr Based Structure Of The Closed Conformation Of Lys48- Linked Di-Ubiquitin Using Experimental Global Rotational Diffusion Ten" 50.00 76 100.00 100.00 1.29e-44
PDB 2QHO "Crystal Structure Of The Uba Domain From Edd Ubiquitin Ligase In Complex With Ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 2RR9 "The Solution Structure Of The K63-Ub2:tuims Complex" 50.00 76 100.00 100.00 1.29e-44
PDB 2RSU "Alternative Structure Of Ubiquitin" 50.00 76 98.68 98.68 6.39e-44
PDB 2RU6 "The Pure Alternative State Of Ubiquitin" 50.00 76 98.68 98.68 6.39e-44
PDB 2W9N "Crystal Structure Of Linear Di-Ubiquitin" 50.00 152 100.00 100.00 1.29e-43
PDB 2WWZ "Tab2 Nzf Domain In Complex With Lys63-Linked Di-Ubiquitin, P212121" 50.00 76 100.00 100.00 1.29e-44
PDB 2WX0 "Tab2 Nzf Domain In Complex With Lys63-Linked Di-Ubiquitin, P21" 50.00 76 100.00 100.00 1.29e-44
PDB 2WX1 "Tab2 Nzf Domain In Complex With Lys63-Linked Tri-Ubiquitin, P212121" 50.00 76 100.00 100.00 1.29e-44
PDB 2XBB "Nedd4 Hect:ub Complex" 50.00 76 100.00 100.00 1.29e-44
PDB 2XEW "Crystal Structure Of K11-Linked Diubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 2XK5 "Crystal Structure Of K6-Linked Diubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 2Y5B "Structure Of Usp21 In Complex With Linear Diubiquitin-Aldehyde" 99.34 152 99.34 99.34 2.00e-100
PDB 2Z59 "Complex Structures Of Mouse Rpn13 (22-130aa) And Ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 2ZCB "Crystal Structure Of Ubiquitin P37aP38A" 50.00 76 97.37 97.37 5.29e-43
PDB 2ZCC "Ubiquitin Crystallized Under High Pressure" 50.00 76 100.00 100.00 1.29e-44
PDB 2ZNV "Crystal Structure Of Human Amsh-Lp Dub Domain In Complex With Lys63-Linked Ubiquitin Dimer" 50.00 77 100.00 100.00 1.49e-44
PDB 2ZVN "Nemo Cozi Domain Incomplex With Diubiquitin In P212121 Space Group" 51.32 154 98.72 98.72 1.75e-44
PDB 2ZVO "Nemo Cozi Domain In Complex With Diubiquitin In C2 Space Group" 51.32 154 98.72 98.72 1.75e-44
PDB 3A1Q "Crystal Structure Of The Mouse Rap80 Uims In Complex With Lys63-Linked Di-Ubiquitin" 50.00 77 100.00 100.00 1.49e-44
PDB 3A33 "Ubch5b~ubiquitin Conjugate" 50.00 76 100.00 100.00 1.29e-44
PDB 3A9J "Crystal Structure Of The Mouse Tab2-Nzf In Complex With Lys63-Linked Di-Ubiquitin" 50.00 77 100.00 100.00 1.49e-44
PDB 3A9K "Crystal Structure Of The Mouse Tab3-Nzf In Complex With Lys63-Linked Di-Ubiquitin" 50.00 77 100.00 100.00 1.49e-44
PDB 3ALB "Cyclic Lys48-Linked Tetraubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 3AUL "Crystal Structure Of Wild-Type Lys48-Linked Diubiquitin In An Open Conformation" 50.00 76 100.00 100.00 1.29e-44
PDB 3AXC "Crystal Structure Of Linear Diubiquitin" 51.32 154 98.72 98.72 1.75e-44
PDB 3B08 "Crystal Structure Of The Mouse Hoil1-l-nzf In Complex With Linear Di- Ubiquitin" 100.00 152 100.00 100.00 2.92e-102
PDB 3B0A "Crystal Structure Of The Mouse Hoil1-l-nzf In Complex With Linear Di- Ubiquitin" 100.00 152 100.00 100.00 2.92e-102
PDB 3DVG "Crystal Structure Of K63-Specific Fab Apu.3a8 Bound To K63-Linked Di- Ubiquitin" 50.00 80 100.00 100.00 1.45e-44
PDB 3DVN "Crystal Structure Of K63-specific Fab Apu2.16 Bound To K63-linked Di- Ubiquitin" 50.00 80 100.00 100.00 1.45e-44
PDB 3EEC "X-Ray Structure Of Human Ubiquitin Cd(Ii) Adduct" 50.00 76 100.00 100.00 1.29e-44
PDB 3EFU "X-Ray Structure Of Human Ubiquitin-Hg(Ii) Adduct" 50.00 76 100.00 100.00 1.29e-44
PDB 3EHV "X-Ray Structure Of Human Ubiquitin Zn(Ii) Adduct" 50.00 76 100.00 100.00 1.29e-44
PDB 3H1U "Structure Of Ubiquitin In Complex With Cd Ions" 50.00 76 100.00 100.00 1.29e-44
PDB 3H7P "Crystal Structure Of K63-Linked Di-Ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 3H7S "Crystal Structures Of K63-Linked Di- And Tri-Ubiquitin Reveal A Highly Extended Chain Architecture" 50.00 76 98.68 98.68 4.72e-42
PDB 3HM3 "The Structure And Conformation Of Lys-63 Linked Tetra-Ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 3JSV "Crystal Structure Of Mouse Nemo Cozi In Complex With Lys63- Linked Di-Ubiquitin" 50.00 77 100.00 100.00 1.49e-44
PDB 3JVZ E2~ubiquitin-Hect 50.00 81 100.00 100.00 1.30e-44
PDB 3JW0 E2~ubiquitin-Hect 50.00 81 100.00 100.00 1.30e-44
PDB 3K9P "The Crystal Structure Of E2-25k And Ubiquitin Complex" 50.00 79 100.00 100.00 1.32e-44
PDB 3M3J "A New Crystal Form Of Lys48-Linked Diubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 3N30 "Crystal Structure Of Cubic Zn3-Hub (Human Ubiquitin) Adduct" 50.00 76 100.00 100.00 1.29e-44
PDB 3N32 "The Crystal Structure Of Human Ubiquitin Adduct With Zeise's Salt" 50.00 76 100.00 100.00 1.29e-44
PDB 3NHE "High Resolution Structure (1.26a) Of Usp2a In Complex With Ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 3NOB "Structure Of K11-linked Di-ubiquitin" 50.00 78 100.00 100.00 1.00e-44
PDB 3NS8 "Crystal Structure Of An Open Conformation Of Lys48-Linked Diubiquitin At Ph 7.5" 50.00 76 100.00 100.00 1.29e-44
PDB 3OFI "Crystal Structure Of Human Insulin-Degrading Enzyme In Complex With Ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 3OJ3 "Crystal Structure Of The A20 Znf4 And Ubiquitin Complex" 50.00 79 100.00 100.00 1.32e-44
PDB 3OJ4 "Crystal Structure Of The A20 Znf4, Ubiquitin And Ubch5a Complex" 50.00 79 100.00 100.00 1.32e-44
PDB 3PHD "Crystal Structure Of Human Hdac6 In Complex With Ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 3PTF "X-Ray Structure Of The Non-Covalent Complex Between Ubch5a And Ubiquitin" 50.00 79 100.00 100.00 1.32e-44
PDB 3TBL "Structure Of Mono-ubiquitinated Pcna: Implications For Dna Polymerase Switching And Okazaki Fragment Maturation" 50.00 76 100.00 100.00 1.29e-44
PDB 3U30 "Crystal Structure Of A Linear-Specific Ubiquitin Fab Bound To Linear Ubiquitin" 100.00 172 100.00 100.00 2.20e-102
PDB 3UGB "Ubch5c~ubiquitin Conjugate" 50.00 76 100.00 100.00 1.29e-44
PDB 3VDZ "Tailoring Encodable Lanthanide-Binding Tags As Mri Contrast Agents: Xq-Dse3-Ubiquitin At 2.4 Angstroms" 50.00 111 100.00 100.00 2.88e-44
PDB 3VHT "Crystal Structure Of Gfp-Wrnip1 Ubz Domain Fusion Protein In Complex With Ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 3VUW "Crystal Structure Of A20 Zf7 In Complex With Linear Ubiquitin, Form I" 50.00 76 100.00 100.00 1.29e-44
PDB 3VUX "Crystal Structure Of A20 Zf7 In Complex With Linear Ubiquitin, Form Ii" 50.00 76 100.00 100.00 1.29e-44
PDB 3VUY "Crystal Structure Of A20 Zf7 In Complex With Linear Tetraubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 3WWQ "Crystal Structure Of Faap20 Ubz Domain In Complex With Lys63-linked Diubiquitin" 50.00 77 100.00 100.00 1.49e-44
PDB 3WXE "Crystal Structure Of Cyld Usp Domain (c596s) In Complex With Met1- Linked Diubiquitin" 50.00 148 100.00 100.00 7.86e-44
PDB 3WXF "Crystal Structure Of Cyld Usp Domain (c596s E674q) In Complex With Met1-linked Diubiquitin" 50.00 148 100.00 100.00 7.86e-44
PDB 3WXG "Crystal Structure Of Cyld Usp Domain (c596a) In Complex With Lys63- Linked Diubiquitin" 50.00 76 98.68 100.00 2.90e-44
PDB 3ZLZ "Lys6-linked Tri-ubiquitin" 50.00 76 98.68 100.00 2.90e-44
PDB 3ZNI "Structure Of Phosphotyr363-cbl-b - Ubch5b-ub - Zap-70 Peptide Complex" 50.00 81 100.00 100.00 1.30e-44
PDB 3ZNZ "Crystal Structure Of Otulin Otu Domain (c129a) In Complex With Met1-di Ubiquitin" 100.00 152 100.00 100.00 2.92e-102
PDB 4A18 "T.Thermophila 60s Ribosomal Subunit In Complex With Initiation Factor 6. This File Contains 26s Rrna And Proteins Of Molecule 1" 50.00 129 97.37 98.68 3.70e-43
PDB 4A19 "T.Thermophila 60s Ribosomal Subunit In Complex With Initiation Factor 6. This File Contains 26s Rrna And Proteins Of Molecule 2" 50.00 129 97.37 98.68 3.70e-43
PDB 4A1B "T.Thermophila 60s Ribosomal Subunit In Complex With Initiation Factor 6. This File Contains 26s Rrna And Proteins Of Molecule 3" 50.00 129 97.37 98.68 3.70e-43
PDB 4A1D "T.Thermophila 60s Ribosomal Subunit In Complex With Initiation Factor 6. This File Contains 26s Rrna And Proteins Of Molecule 4" 50.00 129 97.37 98.68 3.70e-43
PDB 4ADX "The Cryo-em Structure Of The Archaeal 50s Ribosomal Subunit In Complex With Initiation Factor 6" 50.00 129 97.37 98.68 3.70e-43
PDB 4AP4 "Rnf4 - Ubch5a - Ubiquitin Heterotrimeric Complex" 50.00 80 100.00 100.00 1.37e-44
PDB 4AUQ "Structure Of Birc7-Ubch5b-Ub Complex." 50.00 81 100.00 100.00 1.30e-44
PDB 4BBN "Nedd4 Hect-ub:ub Complex" 50.00 76 100.00 100.00 1.29e-44
PDB 4BOS "Structure Of Otud2 Otu Domain In Complex With Ubiquitin K11- Linked Peptide" 50.00 76 100.00 100.00 1.29e-44
PDB 4BOZ "Structure Of Otud2 Otu Domain In Complex With K11-linked Di Ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 4BVU "Structure Of Shigella Effector Ospg In Complex With Host Ubch5c-ubiquitin Conjugate" 50.00 76 100.00 100.00 1.29e-44
PDB 4CXC "Regulation Of The Mammalian Elongation Cycle By 40s Subunit Rolling: A Eukaryotic-specific Ribosome Rearrangement" 50.00 156 100.00 100.00 1.19e-44
PDB 4CXD "Regulation Of The Mammalian Elongation Cycle By 40s Subunit Rolling: A Eukaryotic-specific Ribosome Rearrangement" 50.00 128 100.00 100.00 7.48e-45
PDB 4D5L "Cryo-em Structures Of Ribosomal 80s Complexes With Termination Factors And Cricket Paralysis Virus Ires Reveal The Ires In The " 50.00 156 100.00 100.00 1.19e-44
PDB 4D61 "Cryo-em Structures Of Ribosomal 80s Complexes With Termination Factors And Cricket Paralysis Virus Ires Reveal The Ires In The " 50.00 156 100.00 100.00 1.19e-44
PDB 4DDG "Crystal Structure Of Human Otub1UBCH5B~UBUB" 50.00 76 100.00 100.00 1.29e-44
PDB 4DDI "Crystal Structure Of Human Otub1UBCH5B~UBUB" 50.00 76 100.00 100.00 1.29e-44
PDB 4FJV "Crystal Structure Of Human Otubain2 And Ubiquitin Complex" 50.00 86 100.00 100.00 1.81e-44
PDB 4JIO "Bro1 V Domain And Ubiquitin" 50.00 76 98.68 98.68 8.95e-44
PDB 4JQW "Crystal Structure Of A Complex Of Nod1 Card And Ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 4K1R "Crystal Structure Of Schizosaccharomyces Pombe Sst2 Catalytic Domain And Ubiquitin" 50.00 81 100.00 100.00 2.04e-44
PDB 4K7S "Crystal Structure Of Zn2-hub (human Ubiquitin) Adduct From A Solution 35 Mm Zinc Acetate/1.3 Mm Hub" 50.00 76 100.00 100.00 1.29e-44
PDB 4K7U "Crystal Structure Of Zn2.3-hub (human Ubiquitin) Adduct From A Solution 70 Mm Zinc Acetate/1.3 Mm Hub" 50.00 76 100.00 100.00 1.29e-44
PDB 4K7W "Crystal Structure Of Zn3-hub(human Ubiquitin) Adduct From A Solution 100 Mm Zinc Acetate/1.3 Mm Hub" 50.00 76 100.00 100.00 1.29e-44
PDB 4KSK "Gumby/fam105b In Complex With Ubiquitin" 50.00 80 100.00 100.00 1.37e-44
PDB 4KSL "Gumby/fam105b In Complex With Linear Di-ubiquitin" 50.66 156 100.00 100.00 1.53e-44
PDB 4KZX "Rabbit 40s Ribosomal Subunit In Complex With Eif1." 50.00 156 100.00 100.00 1.19e-44
PDB 4KZY "Rabbit 40s Ribosomal Subunit In Complex With Eif1 And Eif1a." 50.00 156 100.00 100.00 1.19e-44
PDB 4KZZ "Rabbit 40s Ribosomal Subunit In Complex With Mrna, Initiator Trna And Eif1a" 50.00 156 100.00 100.00 1.19e-44
PDB 4LDT "The Structure Of H/ceotub1-ubiquitin Aldehyde-ubch5b~ub" 50.00 76 100.00 100.00 1.29e-44
PDB 4LJO "Structure Of An Active Ligase (hoip)/ubiquitin Transfer Complex" 50.00 76 100.00 100.00 1.29e-44
PDB 4LJP "Structure Of An Active Ligase (hoip-h889a)/ubiquitin Transfer Complex" 50.00 76 100.00 100.00 1.29e-44
PDB 4M0W "Crystal Structure Of Sars-cov Papain-like Protease C112s Mutant In Complex With Ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 4MDK "Cdc34-ubiquitin-cc0651 Complex" 50.00 80 100.00 100.00 1.37e-44
PDB 4MSM "Crystal Structure Of Schizosaccharomyces Pombe Amsh-like Protease Sst2 E286a Mutant Bound To Ubiquitin" 50.00 81 100.00 100.00 2.04e-44
PDB 4MSQ "Crystal Structure Of Schizosaccharomyces Pombe Amsh-like Protease Sst2 Catalytic Domain Bound To Ubiquitin" 50.00 81 100.00 100.00 2.04e-44
PDB 4NQK "Structure Of An Ubiquitin Complex" 50.00 79 100.00 100.00 2.18e-44
PDB 4NQL "The Crystal Structure Of The Dub Domain Of Amsh Orthologue, Sst2 From S. Pombe, In Complex With Lysine 63-linked Diubiquitin" 50.00 77 100.00 100.00 1.49e-44
PDB 4P4H "Caught-in-action Signaling Complex Of Rig-i 2card Domain And Mavs Card Domain" 50.00 79 100.00 100.00 2.18e-44
PDB 4PIG "Crystal Structure Of The Ubiquitin K11s Mutant" 50.00 76 98.68 98.68 4.77e-44
PDB 4PIH "X-ray Crystal Structure Of The K33s Mutant Of Ubiquitin" 50.00 76 98.68 98.68 4.77e-44
PDB 4PQT "Insights Into The Mechanism Of Deubiquitination By Jamm Deubiquitinases From Co-crystal Structures Of Enzyme With Substrate And" 50.00 81 100.00 100.00 2.04e-44
PDB 4R62 "Structure Of Rad6~ub" 50.00 78 100.00 100.00 1.18e-44
PDB 4S1Z "Crystal Structure Of Trabid Nzf1 In Complex With K29 Linked Di- Ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 4S22 "Crystal Structure Of K29 Linked Di-ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 4UN2 "Crystal Structure Of The Uba Domain Of Dsk2 In Complex With Ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 4UPX "Mammalian 80s Hcv-ires Initiation Complex With Eif5b Pre-like State" 50.00 128 100.00 100.00 7.48e-45
PDB 4UQ1 "Mammalian 80s Hcv-ires Initiation Complex With Eif5b Post-like State" 50.00 128 100.00 100.00 7.48e-45
PDB 4UQ4 "Mammalian 80s Hcv-ires Initiation Complex With Eif5b Post-like State" 50.00 156 100.00 100.00 1.19e-44
PDB 4UQ5 "Mammalian 80s Hcv-ires Initiation Complex With Eif5b Pre-like State" 50.00 156 100.00 100.00 1.19e-44
PDB 4V3K "Rnf38-ubch5b-ub Complex" 50.00 81 100.00 100.00 1.30e-44
PDB 4V3L "Rnf38-ub-ubch5b-ub Complex" 50.00 81 100.00 100.00 1.30e-44
PDB 4W20 "Structure Of The Mammalian 60s Ribosomal Subunit (this Entry Contains The Large Ribosomal Proteins)" 50.00 128 100.00 100.00 7.48e-45
PDB 4W22 "Structure Of The 80s Mammalian Ribosome Bound To Eef2 (this Entry Contains The Large Ribosomal Subunit Proteins)" 50.00 128 100.00 100.00 7.48e-45
PDB 4W23 "Structure Of The 80s Mammalian Ribosome Bound To Eef2 (this Entry Contains The Small Ribosomal Subunit)" 50.00 156 100.00 100.00 1.19e-44
PDB 4W25 "Structure Of The Idle Mammalian Ribosome-sec61 Complex (this Entry Contains The Large Ribosomal Subunit Proteins)" 50.00 128 100.00 100.00 7.48e-45
PDB 4W27 "Structure Of The Translating Mammalian Ribosome-sec61 Complex (this Entry Contains The Large Ribosomal Subunit Proteins)" 50.00 128 100.00 100.00 7.48e-45
PDB 4W28 "Structure Of The Translating Mammalian Ribosome-sec61 Complex (this Entry Contains The Small Ribosomal Subunit)" 50.00 156 100.00 100.00 1.19e-44
PDB 4WHV Rnf8/ubc13c87k~ub 50.00 83 100.00 100.00 3.41e-44
PDB 4WLR "Crystal Structure Of Much37-hrpn13 Ctd-hub Complex" 50.00 76 100.00 100.00 1.29e-44
PDB 4WUR "The Crystal Structure Of The Mers-cov Papain-like Protease (c111s) With Human Ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 4WZP "Ser65 Phosphorylated Ubiquitin, Major Conformation" 50.00 76 98.68 98.68 8.39e-44
PDB 4XKL "Crystal Structure Of Ndp52 Zf2 In Complex With Mono-ubiquitin" 50.00 80 100.00 100.00 1.90e-44
PDB 4XOF "Observing The Overall Rocking Motion Of A Protein In A Crystal - Orthorhombic Ubiquitin Crystals Without Zinc." 50.00 76 100.00 100.00 1.29e-44
PDB 4XOK "Observing The Overall Rocking Motion Of A Protein In A Crystal." 50.00 76 100.00 100.00 1.29e-44
PDB 4XOL "Observing The Overall Rocking Motion Of A Protein In A Crystal - Cubic Ubiquitin Crystals." 50.00 76 100.00 100.00 1.29e-44
PDB 4XYZ "Crystal Structure Of K33 Linked Di-ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 4Y1H "Crystal Structure Of K33 Linked Tri-ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 4Z9S "Non-covalent Assembly Of Monoubiquitin That Mimics K11 Poly-ubiquitin" 50.00 76 100.00 100.00 1.29e-44
PDB 4ZFR "Catalytic Domain Of Sst2 F403a Mutant Bound To Ubiquitin" 50.00 81 100.00 100.00 2.04e-44
PDB 4ZFT "Catalytic Domain Of Sst2 F403w Mutant Bound To Ubiquitin" 50.00 81 100.00 100.00 2.04e-44
PDB 4ZPZ "Crystal Structure Of Semi-synthetic Ubiquitin With Phospho-ser65 And Ala46cys" 50.00 76 97.37 97.37 2.65e-43
PDB 5AF4 "Structure Of Lys33-linked Diub" 50.00 76 98.68 100.00 2.90e-44
PDB 5AF6 "Structure Of Lys33-linked Diub Bound To Trabid Nzf1" 50.00 76 98.68 100.00 2.90e-44
PDB 5AIT "A Complex Of Of Rnf4-ring Domain, Ubev2, Ubc13-ub (isopeptide Crosslink)" 50.00 76 100.00 100.00 1.29e-44
PDB 5AIU "A Complex Of Rnf4-ring Domain, Ubc13-ub (isopeptide Crosslink)" 50.00 76 100.00 100.00 1.29e-44
DBJ BAA03983 "polyubiquitin [Rattus norvegicus]" 100.00 305 100.00 100.00 4.92e-100
DBJ BAA09096 "TI-225 [Mus musculus]" 50.00 126 100.00 100.00 9.24e-45
DBJ BAA09860 "polyubiquitin [Homo sapiens]" 51.97 611 100.00 100.00 2.66e-42
DBJ BAA11842 "ubiquitin [Cavia porcellus]" 50.66 311 98.70 100.00 4.62e-42
DBJ BAA11843 "ubiquitin extention protein [Cavia porcellus]" 50.00 156 100.00 100.00 1.19e-44
EMBL CAA25706 "unnamed protein product [Saccharomyces cerevisiae]" 75.00 191 97.37 99.12 1.01e-70
EMBL CAA26488 "unnamed protein product [Gallus gallus]" 52.63 157 98.75 100.00 1.63e-46
EMBL CAA28495 "ubiquitin [Homo sapiens]" 100.00 229 100.00 100.00 3.31e-101
EMBL CAA30183 "unnamed protein product [Dictyostelium discoideum]" 50.00 128 97.37 97.37 1.11e-42
EMBL CAA30815 "unnamed protein product [Cricetulus sp.]" 96.71 223 100.00 100.00 3.65e-97
GB AAA28154 "polyubiquitin [Caenorhabditis elegans]" 100.00 838 98.03 98.68 5.41e-92
GB AAA28997 "ubiquitin [Drosophila melanogaster]" 51.32 231 98.72 100.00 1.16e-43
GB AAA28998 "ubiquitin-hybrid protein precursor [Drosophila melanogaster]" 50.00 156 100.00 100.00 2.01e-44
GB AAA28999 "ubiquitin, partial [Drosophila melanogaster]" 50.00 76 100.00 100.00 1.29e-44
GB AAA29000 "ubiquitin, partial [Drosophila melanogaster]" 50.00 76 98.68 98.68 6.33e-44
PIR I50437 "polyubiquitin 4 - chicken [Gallus gallus]" 100.00 305 100.00 100.00 4.92e-100
PIR I51568 "polyubiquitin - African clawed frog (fragment)" 59.87 167 100.00 100.00 9.79e-55
PIR I65237 "ubiquitin / ribosomal protein L40, cytosolic [validated] - rat" 50.00 128 100.00 100.00 7.48e-45
PIR JN0790 "ubiquitin/ribosomal protein CEP52 fusion protein - Leishmania major" 50.00 128 97.37 98.68 4.09e-44
PIR JT0492 "ubiquitin 2 - Tetrahymena pyriformis (fragment)" 50.00 95 97.37 98.68 2.99e-43
PRF 1101405A "ubiquitin precursor" 75.00 191 97.37 99.12 1.02e-70
PRF 1212243A "ubiquitin S1" 50.00 76 100.00 100.00 1.29e-44
PRF 1212243C "ubiquitin S3" 50.00 76 100.00 100.00 1.29e-44
PRF 1212243F "ubiquitin S6(1)" 50.00 76 97.37 97.37 5.18e-43
PRF 1212243G "ubiquitin S6(2)" 50.00 75 97.37 97.37 2.21e-41
REF NP_001005123 "ubiquitin-60S ribosomal protein L40 [Xenopus (Silurana) tropicalis]" 50.00 128 100.00 100.00 7.48e-45
REF NP_001006688 "ubiquitin C [Xenopus (Silurana) tropicalis]" 50.66 609 98.70 100.00 2.11e-40
REF NP_001009117 "polyubiquitin-B [Pan troglodytes]" 100.00 229 100.00 100.00 3.31e-101
REF NP_001009202 "polyubiquitin-B [Ovis aries]" 100.00 305 99.34 100.00 8.67e-100
REF NP_001009286 "ubiquitin-60S ribosomal protein L40 [Ovis aries]" 50.00 128 100.00 100.00 7.48e-45
SP P0C273 "RecName: Full=Ubiquitin-60S ribosomal protein L40; AltName: Full=Ubiquitin A-52 residue ribosomal protein fusion product 1; Con" 50.00 128 100.00 100.00 7.48e-45
SP P0C275 "RecName: Full=Ubiquitin-60S ribosomal protein L40; AltName: Full=Ubiquitin A-52 residue ribosomal protein fusion product 1; Con" 50.00 128 100.00 100.00 7.48e-45
SP P0C276 "RecName: Full=Ubiquitin-60S ribosomal protein L40; AltName: Full=Ubiquitin A-52 residue ribosomal protein fusion product 1; Con" 50.00 128 100.00 100.00 7.48e-45
SP P0CG47 "RecName: Full=Polyubiquitin-B; Contains: RecName: Full=Ubiquitin; Flags: Precursor" 100.00 229 100.00 100.00 3.31e-101
SP P0CG48 "RecName: Full=Polyubiquitin-C; Contains: RecName: Full=Ubiquitin; Flags: Precursor" 50.66 685 98.70 100.00 3.14e-40
TPD FAA00319 "TPA: polyubiquitin [Cryptococcus neoformans var. neoformans B-3501A]" 100.00 456 97.37 98.68 3.76e-95
TPE CEL68433 "TPA: ubiquitin / ribosomal protein CEP52 fusion protein, putative [Neospora caninum Liverpool]" 50.00 129 98.68 100.00 2.59e-44
TPE CEL70397 "TPA: Ubiquitin, related [Neospora caninum Liverpool]" 100.00 535 98.68 100.00 5.18e-96
TPE CEL75964 "TPA: ubiquitin / ribosomal protein CEP52 fusion protein, putative [Toxoplasma gondii VEG]" 50.00 129 98.68 100.00 2.59e-44
TPE CEL78064 "TPA: polyubiquitin, putative [Toxoplasma gondii VEG]" 100.00 307 98.68 100.00 7.47e-99
TPG DAA18802 "TPA: polyubiquitin [Bos taurus]" 100.00 305 100.00 100.00 4.81e-100
TPG DAA20663 "TPA: ubiquitin C [Bos taurus]" 99.34 314 99.34 100.00 2.38e-98
TPG DAA20672 "TPA: ubiquitin B-like [Bos taurus]" 50.00 77 98.68 98.68 8.16e-44
TPG DAA24675 "TPA: 40S ribosomal protein S27a [Bos taurus]" 50.00 156 100.00 100.00 1.19e-44
TPG DAA28295 "TPA: ubiquitin and ribosomal protein L40 [Bos taurus]" 50.00 128 100.00 100.00 7.48e-45
stop_
save_
####################
# Natural source #
####################
save_natural_source
_Saveframe_category natural_source
loop_
_Mol_label
_Organism_name_common
_NCBI_taxonomy_ID
_Superkingdom
_Kingdom
_Genus
_Species
$entity Human 9606 Eukaryota Metazoa Homo sapiens
stop_
save_
#########################
# Experimental source #
#########################
save_experimental_source
_Saveframe_category experimental_source
loop_
_Mol_label
_Production_method
_Host_organism_name_common
_Genus
_Species
_Strain
_Vector_name
_Details
$entity 'recombinant technology' . Escherichia coli 'BL21(DE3) pJY2' pet3a 'Ubiquitin monomers were expressed in E. coli. The dimer was enzymatically assembled using E1 and Ube2S E2.'
stop_
save_
#####################################
# Sample contents and methodology #
#####################################
########################
# Sample description #
########################
save_sample_1
_Saveframe_category sample
_Sample_type solution
_Details 'K11 diubiquitin'
loop_
_Mol_label
_Concentration_value
_Concentration_value_units
_Isotopic_labeling
$entity 75 uM '[U-99% 15N]'
'sodium phosphate' 20 mM 'natural abundance'
D2O 7 % 'natural abundance'
H2O 93 % 'natural abundance'
'sodium chloride' 150 mM 'natural abundance'
stop_
save_
############################
# Computer software used #
############################
save_TOPSPIN
_Saveframe_category software
_Name TOPSPIN
_Version 2.1
loop_
_Vendor
_Address
_Electronic_address
'Bruker Biospin' . .
stop_
loop_
_Task
collection
stop_
_Details .
save_
save_NMRPipe
_Saveframe_category software
_Name NMRPipe
_Version .
loop_
_Vendor
_Address
_Electronic_address
'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . .
stop_
loop_
_Task
processing
stop_
_Details .
save_
save_SPARKY
_Saveframe_category software
_Name SPARKY
_Version .
loop_
_Vendor
_Address
_Electronic_address
Goddard . .
stop_
loop_
_Task
'chemical shift assignment'
stop_
_Details .
save_
#########################
# Experimental detail #
#########################
##################################
# NMR Spectrometer definitions #
##################################
save_spectrometer_1
_Saveframe_category NMR_spectrometer
_Manufacturer Bruker
_Model Avance
_Field_strength 600
_Details .
save_
#############################
# NMR applied experiments #
#############################
save_2D_1H-15N_HSQC_1
_Saveframe_category NMR_applied_experiment
_Experiment_name '2D 1H-15N HSQC'
_Sample_label $sample_1
save_
save_2D_1H-15N_IPAP_HSQC_2
_Saveframe_category NMR_applied_experiment
_Experiment_name '2D 1H-15N IPAP HSQC'
_Sample_label $sample_1
save_
save_NMR_spectrometer_expt
_Saveframe_category NMR_applied_experiment
_Experiment_name .
_BMRB_pulse_sequence_accession_number .
_Details .
save_
#######################
# Sample conditions #
#######################
save_sample_conditions_1
_Saveframe_category sample_conditions
_Details '150 mM NaCl'
loop_
_Variable_type
_Variable_value
_Variable_value_error
_Variable_value_units
'ionic strength' 150 . mM
pH 6.8 . pH
pressure 1 . atm
temperature 296 . K
stop_
save_
####################
# NMR parameters #
####################
##############################
# Assigned chemical shifts #
##############################
################################
# Chemical shift referencing #
################################
save_chemical_shift_reference_1
_Saveframe_category chemical_shift_reference
_Details .
loop_
_Mol_common_name
_Atom_type
_Atom_isotope_number
_Atom_group
_Chem_shift_units
_Chem_shift_value
_Reference_method
_Reference_type
_External_reference_sample_geometry
_External_reference_location
_External_reference_axis
_Indirect_shift_ratio
water H 1 protons ppm 4.7 internal direct . . . 1.00000000
water N 15 protons ppm 4.7 na indirect . . . 0.10000000
stop_
save_
###################################
# Assigned chemical shift lists #
###################################
###################################################################
# Chemical Shift Ambiguity Index Value Definitions #
# #
# The values other than 1 are used for those atoms with different #
# chemical shifts that cannot be assigned to stereospecific atoms #
# or to specific residues or chains. #
# #
# Index Value Definition #
# #
# 1 Unique (including isolated methyl protons, #
# geminal atoms, and geminal methyl #
# groups with identical chemical shifts) #
# (e.g. ILE HD11, HD12, HD13 protons) #
# 2 Ambiguity of geminal atoms or geminal methyl #
# proton groups (e.g. ASP HB2 and HB3 #
# protons, LEU CD1 and CD2 carbons, or #
# LEU HD11, HD12, HD13 and HD21, HD22, #
# HD23 methyl protons) #
# 3 Aromatic atoms on opposite sides of #
# symmetrical rings (e.g. TYR HE1 and HE2 #
# protons) #
# 4 Intraresidue ambiguities (e.g. LYS HG and #
# HD protons or TRP HZ2 and HZ3 protons) #
# 5 Interresidue ambiguities (LYS 12 vs. LYS 27) #
# 6 Intermolecular ambiguities (e.g. ASP 31 CA #
# in monomer 1 and ASP 31 CA in monomer 2 #
# of an asymmetrical homodimer, duplex #
# DNA assignments, or other assignments #
# that may apply to atoms in one or more #
# molecule in the molecular assembly) #
# 9 Ambiguous, specific ambiguity not defined #
# #
###################################################################
save_assigned_chem_shift_list_1
_Saveframe_category assigned_chemical_shifts
_Details .
loop_
_Experiment_label
'2D 1H-15N HSQC'
stop_
loop_
_Sample_label
$sample_1
stop_
_Sample_conditions_label $sample_conditions_1
_Chem_shift_reference_set_label $chemical_shift_reference_1
_Mol_system_component_name 'K11-linked Diubiquitin_1'
_Text_data_format .
_Text_data .
loop_
_Atom_shift_assign_ID
_Residue_author_seq_code
_Residue_seq_code
_Residue_label
_Atom_name
_Atom_type
_Chem_shift_value
_Chem_shift_value_error
_Chem_shift_ambiguity_code
1 2 2 GLN H H 8.868 . .
2 2 2 GLN N N 123.029 . .
3 3 3 ILE H H 8.284 . .
4 3 3 ILE N N 115.106 . .
5 4 4 PHE H H 8.571 . .
6 4 4 PHE N N 118.718 . .
7 5 5 VAL H H 9.278 . .
8 5 5 VAL N N 121.612 . .
9 6 6 LYS H H 9.032 . .
10 6 6 LYS N N 128.105 . .
11 7 7 THR H H 8.625 . .
12 7 7 THR N N 115.240 . .
13 8 8 LEU H H 9.194 . .
14 8 8 LEU N N 121.612 . .
15 9 9 THR H H 7.618 . .
16 9 9 THR N N 105.816 . .
17 10 10 GLY H H 7.849 . .
18 10 10 GLY N N 109.497 . .
19 11 11 LYS H H 7.252 . .
20 11 11 LYS N N 121.511 . .
21 12 12 THR H H 8.652 . .
22 12 12 THR N N 120.259 . .
23 13 13 ILE H H 9.519 . .
24 13 13 ILE N N 127.689 . .
25 14 14 THR H H 8.712 . .
26 14 14 THR N N 121.636 . .
27 15 15 LEU H H 8.676 . .
28 15 15 LEU N N 125.124 . .
29 16 16 GLU H H 8.114 . .
30 16 16 GLU N N 122.775 . .
31 17 17 VAL H H 8.913 . .
32 17 17 VAL N N 117.682 . .
33 18 18 GLU H H 8.635 . .
34 18 18 GLU N N 119.386 . .
35 20 20 SER H H 7.010 . .
36 20 20 SER N N 103.545 . .
37 21 21 ASP H H 8.034 . .
38 21 21 ASP N N 124.073 . .
39 22 22 THR H H 7.858 . .
40 22 22 THR N N 109.123 . .
41 23 23 ILE H H 8.508 . .
42 23 23 ILE N N 121.422 . .
43 25 25 ASN H H 7.909 . .
44 25 25 ASN N N 121.486 . .
45 26 26 VAL H H 8.100 . .
46 26 26 VAL N N 122.416 . .
47 27 27 LYS H H 8.540 . .
48 27 27 LYS N N 119.120 . .
49 28 28 ALA H H 7.964 . .
50 28 28 ALA N N 123.624 . .
51 29 29 LYS H H 7.849 . .
52 29 29 LYS N N 120.481 . .
53 30 30 ILE H H 8.271 . .
54 30 30 ILE N N 121.521 . .
55 31 31 GLN H H 8.547 . .
56 31 31 GLN N N 123.679 . .
57 32 32 ASP H H 8.031 . .
58 32 32 ASP N N 120.006 . .
59 33 33 LYS H H 7.421 . .
60 33 33 LYS N N 115.673 . .
61 34 34 GLU H H 8.725 . .
62 34 34 GLU N N 114.226 . .
63 35 35 GLY H H 8.498 . .
64 35 35 GLY N N 108.975 . .
65 39 39 ASP H H 8.521 . .
66 39 39 ASP N N 113.761 . .
67 40 40 GLN H H 7.798 . .
68 40 40 GLN N N 116.978 . .
69 41 41 GLN H H 7.472 . .
70 41 41 GLN N N 118.137 . .
71 42 42 ARG H H 8.476 . .
72 42 42 ARG N N 123.155 . .
73 43 43 LEU H H 8.759 . .
74 43 43 LEU N N 124.507 . .
75 44 44 ILE H H 9.108 . .
76 44 44 ILE N N 122.610 . .
77 45 45 PHE H H 8.824 . .
78 45 45 PHE N N 125.092 . .
79 46 46 ALA H H 8.986 . .
80 46 46 ALA N N 133.179 . .
81 47 47 GLY H H 8.117 . .
82 47 47 GLY N N 102.661 . .
83 48 48 LYS H H 7.936 . .
84 48 48 LYS N N 122.016 . .
85 49 49 GLN H H 8.642 . .
86 49 49 GLN N N 123.222 . .
87 50 50 LEU H H 8.531 . .
88 50 50 LEU N N 125.699 . .
89 51 51 GLU H H 8.390 . .
90 51 51 GLU N N 123.318 . .
91 52 52 ASP H H 8.157 . .
92 52 52 ASP N N 120.611 . .
93 54 54 ARG H H 7.434 . .
94 54 54 ARG N N 119.537 . .
95 55 55 THR H H 8.808 . .
96 55 55 THR N N 108.948 . .
97 56 56 LEU H H 8.137 . .
98 56 56 LEU N N 118.142 . .
99 57 57 SER H H 8.441 . .
100 57 57 SER N N 113.575 . .
101 58 58 ASP H H 7.918 . .
102 58 58 ASP N N 124.676 . .
103 59 59 TYR H H 7.235 . .
104 59 59 TYR N N 115.914 . .
105 60 60 ASN H H 8.125 . .
106 60 60 ASN N N 115.972 . .
107 61 61 ILE H H 7.241 . .
108 61 61 ILE N N 119.163 . .
109 62 62 GLN H H 7.643 . .
110 62 62 GLN N N 125.087 . .
111 63 63 LYS H H 8.452 . .
112 63 63 LYS N N 120.602 . .
113 64 64 GLU H H 9.287 . .
114 64 64 GLU N N 115.358 . .
115 65 65 SER H H 7.724 . .
116 65 65 SER N N 115.187 . .
117 66 66 THR H H 8.681 . .
118 66 66 THR N N 117.545 . .
119 67 67 LEU H H 9.377 . .
120 67 67 LEU N N 127.981 . .
121 68 68 HIS H H 9.223 . .
122 68 68 HIS N N 119.683 . .
123 69 69 LEU H H 8.272 . .
124 69 69 LEU N N 123.979 . .
125 70 70 VAL H H 9.145 . .
126 70 70 VAL N N 126.790 . .
127 71 71 LEU H H 8.187 . .
128 71 71 LEU N N 123.635 . .
129 72 72 ARG H H 8.518 . .
130 72 72 ARG N N 123.425 . .
131 73 73 LEU H H 8.307 . .
132 73 73 LEU N N 123.973 . .
133 74 74 ARG H H 8.589 . .
134 74 74 ARG N N 122.227 . .
135 75 75 GLY H H 8.655 . .
136 75 75 GLY N N 110.300 . .
137 76 76 GLY H H 8.295 . .
138 76 76 GLY N N 108.902 . .
stop_
save_
save_chainB
_Saveframe_category assigned_chemical_shifts
_Details .
loop_
_Experiment_label
'2D 1H-15N HSQC'
stop_
loop_
_Sample_label
$sample_1
stop_
_Sample_conditions_label $sample_conditions_1
_Chem_shift_reference_set_label $chemical_shift_reference_1
_Mol_system_component_name 'K11-linked Diubiquitin_2'
_Text_data_format .
_Text_data .
loop_
_Atom_shift_assign_ID
_Residue_author_seq_code
_Residue_seq_code
_Residue_label
_Atom_name
_Atom_type
_Chem_shift_value
_Chem_shift_value_error
_Chem_shift_ambiguity_code
1 2 2 GLN H H 8.880 . .
2 2 2 GLN N N 123.236 . .
3 3 3 ILE H H 8.279 . .
4 3 3 ILE N N 115.200 . .
5 4 4 PHE H H 8.557 . .
6 4 4 PHE N N 118.584 . .
7 5 5 VAL H H 9.264 . .
8 5 5 VAL N N 121.742 . .
9 6 6 LYS H H 8.973 . .
10 6 6 LYS N N 127.775 . .
11 7 7 THR H H 8.643 . .
12 7 7 THR N N 114.483 . .
13 8 8 LEU H H 9.243 . .
14 8 8 LEU N N 121.566 . .
15 9 9 THR H H 7.588 . .
16 9 9 THR N N 105.794 . .
17 10 10 GLY H H 7.756 . .
18 10 10 GLY N N 109.060 . .
19 11 11 LYS H H 7.256 . .
20 11 11 LYS N N 122.217 . .
21 12 12 THR H H 8.518 . .
22 12 12 THR N N 119.619 . .
23 13 13 ILE H H 9.540 . .
24 13 13 ILE N N 127.701 . .
25 14 14 THR H H 8.725 . .
26 14 14 THR N N 121.866 . .
27 15 15 LEU H H 8.640 . .
28 15 15 LEU N N 125.088 . .
29 16 16 GLU H H 8.108 . .
30 16 16 GLU N N 122.786 . .
31 17 17 VAL H H 8.917 . .
32 17 17 VAL N N 117.749 . .
33 18 18 GLU H H 8.630 . .
34 18 18 GLU N N 119.401 . .
35 20 20 SER H H 7.003 . .
36 20 20 SER N N 103.525 . .
37 21 21 ASP H H 8.033 . .
38 21 21 ASP N N 124.103 . .
39 22 22 THR H H 7.858 . .
40 22 22 THR N N 109.129 . .
41 23 23 ILE H H 8.495 . .
42 23 23 ILE N N 121.543 . .
43 25 25 ASN H H 7.904 . .
44 25 25 ASN N N 121.427 . .
45 26 26 VAL H H 8.106 . .
46 26 26 VAL N N 122.552 . .
47 27 27 LYS H H 8.494 . .
48 27 27 LYS N N 119.073 . .
49 28 28 ALA H H 7.958 . .
50 28 28 ALA N N 123.429 . .
51 29 29 LYS H H 7.905 . .
52 29 29 LYS N N 120.622 . .
53 30 30 ILE H H 8.233 . .
54 30 30 ILE N N 121.781 . .
55 31 31 GLN H H 8.453 . .
56 31 31 GLN N N 123.419 . .
57 32 32 ASP H H 8.130 . .
58 32 32 ASP N N 120.245 . .
59 33 33 LYS H H 7.526 . .
60 33 33 LYS N N 115.791 . .
61 34 34 GLU H H 8.601 . .
62 34 34 GLU N N 113.553 . .
63 35 35 GLY H H 8.480 . .
64 35 35 GLY N N 109.192 . .
65 39 39 ASP H H 8.517 . .
66 39 39 ASP N N 113.827 . .
67 40 40 GLN H H 7.755 . .
68 40 40 GLN N N 116.970 . .
69 41 41 GLN H H 7.458 . .
70 41 41 GLN N N 118.013 . .
71 42 42 ARG H H 8.453 . .
72 42 42 ARG N N 123.419 . .
73 43 43 LEU H H 8.738 . .
74 43 43 LEU N N 124.728 . .
75 44 44 ILE H H 9.138 . .
76 44 44 ILE N N 122.673 . .
77 45 45 PHE H H 8.791 . .
78 45 45 PHE N N 124.936 . .
79 46 46 ALA H H 8.963 . .
80 46 46 ALA N N 133.183 . .
81 47 47 GLY H H 8.115 . .
82 47 47 GLY N N 102.679 . .
83 48 48 LYS H H 7.925 . .
84 48 48 LYS N N 122.115 . .
85 49 49 GLN H H 8.635 . .
86 49 49 GLN N N 123.259 . .
87 50 50 LEU H H 8.534 . .
88 50 50 LEU N N 125.722 . .
89 51 51 GLU H H 8.389 . .
90 51 51 GLU N N 123.340 . .
91 52 52 ASP H H 8.163 . .
92 52 52 ASP N N 120.653 . .
93 54 54 ARG H H 7.441 . .
94 54 54 ARG N N 119.577 . .
95 55 55 THR H H 8.790 . .
96 55 55 THR N N 108.870 . .
97 56 56 LEU H H 8.128 . .
98 56 56 LEU N N 118.171 . .
99 57 57 SER H H 8.453 . .
100 57 57 SER N N 113.583 . .
101 58 58 ASP H H 7.916 . .
102 58 58 ASP N N 124.699 . .
103 59 59 TYR H H 7.228 . .
104 59 59 TYR N N 115.935 . .
105 60 60 ASN H H 8.119 . .
106 60 60 ASN N N 115.961 . .
107 61 61 ILE H H 7.234 . .
108 61 61 ILE N N 119.171 . .
109 62 62 GLN H H 7.640 . .
110 62 62 GLN N N 125.047 . .
111 63 63 LYS H H 8.450 . .
112 63 63 LYS N N 120.600 . .
113 64 64 GLU H H 9.286 . .
114 64 64 GLU N N 115.431 . .
115 65 65 SER H H 7.732 . .
116 65 65 SER N N 115.204 . .
117 66 66 THR H H 8.670 . .
118 66 66 THR N N 117.513 . .
119 67 67 LEU H H 9.384 . .
120 67 67 LEU N N 128.045 . .
121 68 68 HIS H H 9.198 . .
122 68 68 HIS N N 119.763 . .
123 69 69 LEU H H 8.314 . .
124 69 69 LEU N N 124.082 . .
125 70 70 VAL H H 9.145 . .
126 70 70 VAL N N 126.987 . .
127 71 71 LEU H H 8.203 . .
128 71 71 LEU N N 124.144 . .
129 72 72 ARG H H 8.550 . .
130 72 72 ARG N N 124.225 . .
131 73 73 LEU H H 8.334 . .
132 73 73 LEU N N 124.375 . .
133 74 74 ARG H H 8.487 . .
134 74 74 ARG N N 121.748 . .
135 75 75 GLY H H 8.576 . .
136 75 75 GLY N N 110.516 . .
137 76 76 GLY H H 8.178 . .
138 76 76 GLY N N 109.292 . .
stop_
save_