data_19447 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; DISTINCT UBIQUITIN BINDING MODES EXHIBITED BY SH3 DOMAINS: MOLECULAR DETERMINANTS AND FUNCTIONAL IMPLICATIONS ; _BMRB_accession_number 19447 _BMRB_flat_file_name bmr19447.str _Entry_type original _Submission_date 2013-08-22 _Accession_date 2013-08-22 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Ortega-Roldan J. . . 2 Salmon L. . . 3 Azuaga A. . . 4 Blackledge M. . . 5 'Van Nuland' N. . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 2 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 132 "15N chemical shifts" 132 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2014-02-03 original author . stop_ _Original_release_date 2014-02-03 save_ ############################# # Citation for this entry # ############################# save_citations _Saveframe_category entry_citation _Citation_full . _Citation_title 'Distinct ubiquitin binding modes exhibited by SH3 domains: molecular determinants and functional implications' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 24039852 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Ortega-Roldan J. . . 2 Casares S. . . 3 Ringkjobing-Jensen M. . . 4 Cardenes N. . . 5 Bravo N. . . 6 Blackledge M. . . 7 Azuaga A. . . 8 'Van Nuland' N. . . stop_ _Journal_abbreviation 'PLoS One' _Journal_volume 8 _Journal_issue 9 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first e73018 _Page_last e73018 _Year 2013 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'Ubiquitin Binding with SH3 domains' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label SH3A $SH3A Ubiquitin $Ubiquitin stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_SH3A _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common SH3A _Molecular_mass 6764.630 _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 61 _Mol_residue_sequence ; VDYIVEYDYDAVHDDELTIR VGEIIRNVKKLQEEGWLEGE LNGRRGMFPDNFVKEIKRET E ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 2 VAL 2 3 ASP 3 4 TYR 4 5 ILE 5 6 VAL 6 7 GLU 7 8 TYR 8 9 ASP 9 10 TYR 10 11 ASP 11 12 ALA 12 13 VAL 13 14 HIS 14 15 ASP 15 16 ASP 16 17 GLU 17 18 LEU 18 19 THR 19 20 ILE 20 21 ARG 21 22 VAL 22 23 GLY 23 24 GLU 24 25 ILE 25 26 ILE 26 27 ARG 27 28 ASN 28 29 VAL 29 30 LYS 30 31 LYS 31 32 LEU 32 33 GLN 33 34 GLU 34 35 GLU 35 36 GLY 36 37 TRP 37 38 LEU 38 39 GLU 39 40 GLY 40 41 GLU 41 42 LEU 42 43 ASN 43 44 GLY 44 45 ARG 45 46 ARG 46 47 GLY 47 48 MET 48 49 PHE 49 50 PRO 50 51 ASP 51 52 ASN 52 53 PHE 53 54 VAL 54 55 LYS 55 56 GLU 56 57 ILE 57 58 LYS 58 59 ARG 59 60 GLU 60 61 THR 61 62 GLU stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-11-04 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 16641 SH3-A 100.00 61 100.00 100.00 2.43e-34 PDB 2J6F "N-Terminal Sh3 Domain Of Cms (Cd2ap Human Homolog) Bound To Cbl-B Peptide" 100.00 62 100.00 100.00 2.21e-34 PDB 2J6K "N-Terminal Sh3 Domain Of Cms (Cd2ap Human Homolog)" 100.00 62 100.00 100.00 2.21e-34 PDB 2J6O "Atypical Polyproline Recognition By The Cms N-Terminal Sh3 Domain. Cms:cd2 Heterotrimer" 100.00 62 100.00 100.00 2.21e-34 PDB 2J7I "Atypical Polyproline Recognition By The Cms N-Terminal Sh3 Domain. Cms:cd2 Heterodimer" 100.00 62 100.00 100.00 2.21e-34 PDB 2KRM "Rdc Refined Solution Structure Of The First Sh3 Domain Of Cd2ap" 93.44 57 100.00 100.00 1.68e-31 PDB 2MCN "Distinct Ubiquitin Binding Modes Exhibited By Sh3 Domains: Molecular Determinants And Functional Implications" 93.44 57 100.00 100.00 1.68e-31 PDB 4WCI "Crystal Structure Of The 1st Sh3 Domain From Human Cd2ap (cms) In Complex With A Proline-rich Peptide (aa 378-393) From Human R" 96.72 65 100.00 100.00 7.20e-33 GB ELW65647 "CD2-associated protein [Tupaia chinensis]" 91.80 268 98.21 98.21 1.05e-28 stop_ save_ save_Ubiquitin _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common Ubiquitin _Molecular_mass 8576.914 _Mol_thiol_state 'not present' _Details . _Residue_count 76 _Mol_residue_sequence ; MQIFVKTLTGKTITLEVEPS DTIENVKAKIQDKEGIPPDQ QRLIFAGKQLEDGRTLSDYN IQKESTLHLVLRLRGG ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 1 MET 2 2 GLN 3 3 ILE 4 4 PHE 5 5 VAL 6 6 LYS 7 7 THR 8 8 LEU 9 9 THR 10 10 GLY 11 11 LYS 12 12 THR 13 13 ILE 14 14 THR 15 15 LEU 16 16 GLU 17 17 VAL 18 18 GLU 19 19 PRO 20 20 SER 21 21 ASP 22 22 THR 23 23 ILE 24 24 GLU 25 25 ASN 26 26 VAL 27 27 LYS 28 28 ALA 29 29 LYS 30 30 ILE 31 31 GLN 32 32 ASP 33 33 LYS 34 34 GLU 35 35 GLY 36 36 ILE 37 37 PRO 38 38 PRO 39 39 ASP 40 40 GLN 41 41 GLN 42 42 ARG 43 43 LEU 44 44 ILE 45 45 PHE 46 46 ALA 47 47 GLY 48 48 LYS 49 49 GLN 50 50 LEU 51 51 GLU 52 52 ASP 53 53 GLY 54 54 ARG 55 55 THR 56 56 LEU 57 57 SER 58 58 ASP 59 59 TYR 60 60 ASN 61 61 ILE 62 62 GLN 63 63 LYS 64 64 GLU 65 65 SER 66 66 THR 67 67 LEU 68 68 HIS 69 69 LEU 70 70 VAL 71 71 LEU 72 72 ARG 73 73 LEU 74 74 ARG 75 75 GLY 76 76 GLY stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-01-29 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 11505 entity 100.00 76 98.68 98.68 2.41e-45 BMRB 11547 ubiquitin 100.00 76 98.68 98.68 2.41e-45 BMRB 15047 denatured_ubiquitin 100.00 76 100.00 100.00 4.45e-46 BMRB 15410 Ubi 100.00 76 100.00 100.00 4.45e-46 BMRB 15689 UBB 98.68 103 98.67 100.00 4.23e-44 BMRB 15866 ubiquitin 98.68 76 100.00 100.00 2.71e-45 BMRB 15907 Ubiquitin 100.00 76 100.00 100.00 4.45e-46 BMRB 16228 ubiquitin 100.00 76 97.37 98.68 1.18e-44 BMRB 16582 Ubiquitin 100.00 76 100.00 100.00 4.45e-46 BMRB 16626 Ubiquitin 100.00 76 100.00 100.00 4.45e-46 BMRB 16763 ubiquitin 100.00 76 100.00 100.00 4.45e-46 BMRB 16880 Ubiquitin 100.00 76 100.00 100.00 4.45e-46 BMRB 16885 Ubiquitin 100.00 76 100.00 100.00 4.45e-46 BMRB 16895 UBB+1 98.68 103 98.67 100.00 4.23e-44 BMRB 17059 ubiquitin 100.00 156 100.00 100.00 5.21e-46 BMRB 17181 ubiquitin 100.00 76 100.00 100.00 4.45e-46 BMRB 17239 ubiquitin 100.00 76 100.00 100.00 4.45e-46 BMRB 17333 UB 100.00 76 100.00 100.00 4.45e-46 BMRB 17439 ubiquitin 100.00 76 100.00 100.00 4.45e-46 BMRB 17769 Ubiquitin 100.00 76 100.00 100.00 4.45e-46 BMRB 17919 entity 100.00 76 100.00 100.00 4.45e-46 BMRB 18582 ubiquitin 100.00 76 100.00 100.00 4.45e-46 BMRB 18583 ubiquitin 100.00 76 100.00 100.00 4.45e-46 BMRB 18584 ubiquitin 100.00 76 100.00 100.00 4.45e-46 BMRB 18610 Ubiquitin 100.00 76 100.00 100.00 4.45e-46 BMRB 18611 Ubiquitin_A_state 100.00 76 100.00 100.00 4.45e-46 BMRB 18737 UBIQUITIN 100.00 76 100.00 100.00 4.45e-46 BMRB 19394 ubiquitin 100.00 79 100.00 100.00 4.53e-46 BMRB 19399 Ubiquitin 100.00 76 100.00 100.00 4.45e-46 BMRB 19406 entity 100.00 152 100.00 100.00 4.37e-45 BMRB 19412 entity 100.00 152 100.00 100.00 4.37e-45 BMRB 25070 Ubiquitin 100.00 79 100.00 100.00 4.53e-46 BMRB 25230 Ubiquitin 100.00 78 100.00 100.00 3.66e-46 BMRB 4245 ubiquitin 100.00 76 100.00 100.00 4.45e-46 BMRB 4375 Ubiquitin 100.00 76 100.00 100.00 4.45e-46 BMRB 4983 Ubiquitin 98.68 76 97.33 100.00 3.66e-44 BMRB 5101 uq1_51 67.11 53 100.00 100.00 2.13e-26 BMRB 5387 ubq 100.00 76 100.00 100.00 4.45e-46 BMRB 6457 Ub 100.00 76 100.00 100.00 4.45e-46 BMRB 6466 Ub 100.00 76 100.00 100.00 4.45e-46 BMRB 6470 Ub 100.00 76 100.00 100.00 4.45e-46 BMRB 6488 Ub 100.00 76 100.00 100.00 4.45e-46 BMRB 68 ubiquitin 100.00 76 100.00 100.00 4.45e-46 BMRB 7111 human_ubiquitin 100.00 76 100.00 100.00 4.45e-46 PDB 1AAR "Structure Of A Diubiquitin Conjugate And A Model For Interaction With Ubiquitin Conjugating Enzyme (E2)" 100.00 76 100.00 100.00 4.45e-46 PDB 1CMX "Structural Basis For The Specificity Of Ubiquitin C- Terminal Hydrolases" 98.68 76 100.00 100.00 2.99e-45 PDB 1D3Z "Ubiquitin Nmr Structure" 100.00 76 100.00 100.00 4.45e-46 PDB 1F9J "Structure Of A New Crystal Form Of Tetraubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 1FXT "Structure Of A Conjugating Enzyme-Ubiquitin Thiolester Complex" 100.00 76 100.00 100.00 4.45e-46 PDB 1G6J "Structure Of Recombinant Human Ubiquitin In Aot Reverse Micelles" 98.68 76 100.00 100.00 5.16e-45 PDB 1GJZ "Solution Structure Of A Dimeric N-Terminal Fragment Of Human Ubiquitin" 67.11 53 100.00 100.00 2.13e-26 PDB 1NBF "Crystal Structure Of A Ubp-Family Deubiquitinating Enzyme In Isolation And In Complex With Ubiquitin Aldehyde" 98.68 76 100.00 100.00 2.99e-45 PDB 1OGW "Synthetic Ubiquitin With Fluoro-Leu At 50 And 67" 100.00 76 97.37 97.37 1.80e-44 PDB 1P3Q "Mechanism Of Ubiquitin Recognition By The Cue Domain Of Vps9" 100.00 76 100.00 100.00 4.45e-46 PDB 1Q5W "Ubiquitin Recognition By Npl4 Zinc-Fingers" 100.00 76 100.00 100.00 4.45e-46 PDB 1S1Q "Tsg101(Uev) Domain In Complex With Ubiquitin" 98.68 76 100.00 100.00 5.16e-45 PDB 1TBE "Structure Of Tetraubiquitin Shows How Multiubiquitin Chains Can Be Formed" 100.00 76 100.00 100.00 4.45e-46 PDB 1UBI "Synthetic Structural And Biological Studies Of The Ubiquitin System. Part 1" 100.00 76 100.00 100.00 4.45e-46 PDB 1UBQ "Structure Of Ubiquitin Refined At 1.8 Angstroms Resolution" 100.00 76 100.00 100.00 4.45e-46 PDB 1UZX "A Complex Of The Vps23 Uev With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 1V80 "Solution Structures Of Ubiquitin At 30 Bar And 3 Kbar" 100.00 76 100.00 100.00 4.45e-46 PDB 1V81 "Solution Structures Of Ubiquitin At 30 Bar And 3 Kbar" 100.00 76 100.00 100.00 4.45e-46 PDB 1VX7 "Cryo-em Structure Of The Plasmodium Falciparum 80s Ribosome Bound To The Anti-protozoan Drug Emetine, Large Subunit (protein On" 100.00 128 98.68 100.00 9.37e-46 PDB 1WR6 "Crystal Structure Of Gga3 Gat Domain In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 1WRD "Crystal Structure Of Tom1 Gat Domain In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 1XD3 "Crystal Structure Of Uchl3-Ubvme Complex" 98.68 75 100.00 100.00 2.91e-45 PDB 1XQQ "Simultaneous Determination Of Protein Structure And Dynamics" 100.00 76 100.00 100.00 4.45e-46 PDB 1YD8 "Complex Of Human Gga3 Gat Domain And Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 1YIW "X-Ray Crystal Structure Of A Chemically Synthesized Ubiquitin" 100.00 76 98.68 100.00 1.94e-45 PDB 1YJ1 "X-Ray Crystal Structure Of A Chemically Synthesized [d-Gln35]ubiquitin" 100.00 76 97.37 98.68 2.29e-44 PDB 1YX5 "Solution Structure Of S5a Uim-1UBIQUITIN COMPLEX" 100.00 98 100.00 100.00 1.57e-46 PDB 1YX6 "Solution Structure Of S5a Uim-2UBIQUITIN COMPLEX" 100.00 98 100.00 100.00 1.57e-46 PDB 1ZGU "Solution Structure Of The Human Mms2-Ubiquitin Complex" 100.00 76 98.68 100.00 1.06e-45 PDB 2AYO "Structure Of Usp14 Bound To Ubquitin Aldehyde" 98.68 76 100.00 100.00 2.99e-45 PDB 2BGF "Nmr Structure Of Lys48-Linked Di-Ubiquitin Using Chemical Shift Perturbation Data Together With Rdcs And 15n- Relaxation Data" 100.00 76 100.00 100.00 4.45e-46 PDB 2C7M "Human Rabex-5 Residues 1-74 In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2C7N "Human Rabex-5 Residues 1-74 In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2D3G "Double Sided Ubiquitin Binding Of Hrs-Uim" 100.00 76 100.00 100.00 4.45e-46 PDB 2DEN "Solution Structure Of The Ubiquitin-Associated Domain Of Human Bmsc-Ubp And Its Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2DX5 "The Complex Structure Between The Mouse Eap45-Glue Domain And Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2FCM "X-Ray Crystal Structure Of A Chemically Synthesized [d-Gln35]ubiquitin With A Cubic Space Group" 100.00 76 97.37 98.68 2.29e-44 PDB 2FCN "X-Ray Crystal Structure Of A Chemically Synthesized [d-Val35]ubiquitin With A Cubic Space Group" 100.00 76 97.37 98.68 2.29e-44 PDB 2FCQ "X-Ray Crystal Structure Of A Chemically Synthesized Ubiquitin With A Cubic Space Group" 100.00 76 98.68 100.00 1.94e-45 PDB 2FCS "X-Ray Crystal Structure Of A Chemically Synthesized [l-Gln35]ubiquitin With A Cubic Space Group" 100.00 76 97.37 98.68 2.61e-44 PDB 2FID "Crystal Structure Of A Bovine Rabex-5 Fragment Complexed With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2FIF "Crystal Structure Of A Bovine Rabex-5 Fragment Complexed With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2FUH "Solution Structure Of The Ubch5cUB NON-Covalent Complex" 100.00 76 100.00 100.00 4.45e-46 PDB 2G45 "Co-Crystal Structure Of Znf Ubp Domain From The Deubiquitinating Enzyme Isopeptidase T (Isot) In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2GMI Mms2UBC13~UBIQUITIN 100.00 76 100.00 100.00 4.45e-46 PDB 2HD5 "Usp2 In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2HTH "Structural Basis For Ubiquitin Recognition By The Human Eap45ESCRT-Ii Glue Domain" 100.00 76 100.00 100.00 4.45e-46 PDB 2IBI "Covalent Ubiquitin-Usp2 Complex" 98.68 75 100.00 100.00 2.91e-45 PDB 2J7Q "Crystal Structure Of The Ubiquitin-Specific Protease Encoded By Murine Cytomegalovirus Tegument Protein M48 In Complex With A U" 98.68 75 100.00 100.00 2.91e-45 PDB 2JF5 "Crystal Structure Of Lys63-Linked Di-Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2JRI "Solution Structure Of The Josephin Domain Of Ataxin-3 In Complex With Ubiquitin Molecule." 100.00 76 100.00 100.00 4.45e-46 PDB 2JY6 "Solution Structure Of The Complex Of Ubiquitin And Ubiquilin 1 Uba Domain" 100.00 76 100.00 100.00 4.45e-46 PDB 2JZZ "Solid-State Nmr Structure Of Microcrystalline Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2K25 "Automated Nmr Structure Of The Ubb By Fapsy" 98.68 103 98.67 100.00 4.23e-44 PDB 2K39 "Recognition Dynamics Up To Microseconds Revealed From Rdc Derived Ubiquitin Ensemble In Solution" 100.00 76 100.00 100.00 4.45e-46 PDB 2K6D "Cin85 Sh3-C Domain In Complex With Ubiquitin" 98.68 76 100.00 100.00 2.71e-45 PDB 2K8B "Solution Structure Of Plaa Family Ubiquitin Binding Domain (Pfuc) Cis Isomer In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2K8C "Solution Structure Of Plaa Family Ubiquitin Binding Domain (Pfuc) Trans Isomer In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2KDE "Nmr Structure Of Major S5a (196-306):k48 Linked Diubiquitin Species" 100.00 76 100.00 100.00 4.45e-46 PDB 2KDF "Nmr Structure Of Minor S5a (196-306):k48 Linked Diubiquitin Species" 100.00 76 100.00 100.00 4.45e-46 PDB 2KHW "Solution Structure Of The Human Polymerase Iota Ubm2- Ubiquitin Complex" 100.00 79 100.00 100.00 4.53e-46 PDB 2KJH "Nmr Based Structural Model Of The Ubch8-Ubiquitin Complex" 98.68 76 100.00 100.00 2.71e-45 PDB 2KLG "Pere Nmr Structure Of Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2KN5 "A Correspondence Between Solution-State Dynamics Of An Individual Protein And The Sequence And Conformational Diversity Of Its " 100.00 76 100.00 100.00 4.45e-46 PDB 2KOX "Nmr Residual Dipolar Couplings Identify Long Range Correlated Motions In The Backbone Of The Protein Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2KTF "Solution Nmr Structure Of Human Polymerase Iota Ubm2 In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2KWU "Solution Structure Of Ubm2 Of Murine Polymerase Iota In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2KWV "Solution Structure Of Ubm1 Of Murine Polymerase Iota In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2KX0 "The Solution Structure Of Ubb+1, Frameshift Mutant Of Ubiquitin B" 98.68 103 98.67 100.00 4.23e-44 PDB 2L0F "Solution Nmr Structure Of Human Polymerase Iota Ubm2 (P692a Mutant) In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2L0T "Solution Structure Of The Complex Of Ubiquitin And The Vhs Domain Of Stam2" 100.00 76 100.00 100.00 4.45e-46 PDB 2L3Z "Proton-Detected 4d Dream Solid-State Nmr Structure Of Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2LD9 "Backbone Structure Of Ubiquitin Determined Using Backbone Amide Noes And Backbone N-H And N-C Rdcs" 100.00 77 100.00 100.00 5.39e-46 PDB 2LJ5 "Description Of The Structural Fluctuations Of Proteins From Structure- Based Calculations Of Residual Dipolar Couplings" 100.00 76 100.00 100.00 4.45e-46 PDB 2LVO "Structure Of The Gp78cue Domain Bound To Monubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2LVP "Gp78cue Domain Bound To The Distal Ubiquitin Of K48-Linked Diubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2LVQ "Gp78cue Domain Bound To The Proximal Ubiquitin Of K48-Linked Diubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2LZ6 "Distinct Ubiquitin Binding Modes Exhibited By Sh3 Domains: Molecular Determinants And Functional Implications" 100.00 76 100.00 100.00 4.45e-46 PDB 2MBB "Solution Structure Of The Human Polymerase Iota Ubm1-ubiquitin Complex" 100.00 78 100.00 100.00 3.66e-46 PDB 2MBH "Nmr Structure Of Eklf(22-40)/ubiquitin Complex" 100.00 76 100.00 100.00 4.45e-46 PDB 2MBO "K11-linked Diubiquitin Average Solution Structure At Ph 6.8, 0 Mm Nacl" 100.00 76 100.00 100.00 4.45e-46 PDB 2MBQ "K11-linked Diubiquitin Average Solution Structure At Ph 6.8, 150 Mm Nacl" 100.00 76 100.00 100.00 4.45e-46 PDB 2MCN "Distinct Ubiquitin Binding Modes Exhibited By Sh3 Domains: Molecular Determinants And Functional Implications" 100.00 76 100.00 100.00 4.45e-46 PDB 2MJ5 "Structure Of The Uba Domain Of Human Nbr1 In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2MJB "Solution Nmr Structure Of Ubiquitin Refined Against Dipolar Couplings In 4 Media" 100.00 76 100.00 100.00 4.45e-46 PDB 2MOR "A Tensor-free Method For The Structural And Dynamical Refinement Of Proteins Using Residual Dipolar Couplings" 100.00 76 100.00 100.00 4.45e-46 PDB 2MRE "Nmr Structure Of The Rad18-ubz/ubiquitin Complex" 100.00 79 100.00 100.00 4.53e-46 PDB 2MUR "Solution Structure Of The Human Faap20 Ubz-ubiquitin Complex" 100.00 78 100.00 100.00 3.66e-46 PDB 2NR2 "The Mumo (Minimal Under-Restraining Minimal Over- Restraining) Method For The Determination Of Native States Ensembles Of Prote" 100.00 76 100.00 100.00 4.45e-46 PDB 2O6V "Crystal Structure And Solution Nmr Studies Of Lys48-Linked Tetraubiquitin At Neutral Ph" 100.00 76 100.00 100.00 4.45e-46 PDB 2OJR "Structure Of Ubiquitin Solved By Sad Using The Lanthanide- Binding Tag" 100.00 111 100.00 100.00 1.56e-45 PDB 2OOB "Crystal Structure Of The Uba Domain From Cbl-B Ubiquitin Ligase In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2PE9 "Nmr Based Structure Of The Open Conformation Of Lys48- Linked Di-Ubiquitin Using Experimental Global Rotational Diffusion Tenso" 100.00 76 100.00 100.00 4.45e-46 PDB 2PEA "Nmr Based Structure Of The Closed Conformation Of Lys48- Linked Di-Ubiquitin Using Experimental Global Rotational Diffusion Ten" 100.00 76 100.00 100.00 4.45e-46 PDB 2QHO "Crystal Structure Of The Uba Domain From Edd Ubiquitin Ligase In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2RR9 "The Solution Structure Of The K63-Ub2:tuims Complex" 100.00 76 100.00 100.00 4.45e-46 PDB 2RSU "Alternative Structure Of Ubiquitin" 100.00 76 98.68 98.68 2.41e-45 PDB 2RU6 "The Pure Alternative State Of Ubiquitin" 100.00 76 98.68 98.68 2.41e-45 PDB 2W9N "Crystal Structure Of Linear Di-Ubiquitin" 98.68 152 100.00 100.00 3.82e-44 PDB 2WDT "Crystal Structure Of Plasmodium Falciparum Uchl3 In Complex With The Suicide Inhibitor Ubvme" 98.68 76 100.00 100.00 2.99e-45 PDB 2WWZ "Tab2 Nzf Domain In Complex With Lys63-Linked Di-Ubiquitin, P212121" 100.00 76 100.00 100.00 4.45e-46 PDB 2WX0 "Tab2 Nzf Domain In Complex With Lys63-Linked Di-Ubiquitin, P21" 100.00 76 100.00 100.00 4.45e-46 PDB 2WX1 "Tab2 Nzf Domain In Complex With Lys63-Linked Tri-Ubiquitin, P212121" 100.00 76 100.00 100.00 4.45e-46 PDB 2XBB "Nedd4 Hect:ub Complex" 100.00 76 100.00 100.00 4.45e-46 PDB 2XEW "Crystal Structure Of K11-Linked Diubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2XK5 "Crystal Structure Of K6-Linked Diubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2Y5B "Structure Of Usp21 In Complex With Linear Diubiquitin-Aldehyde" 98.68 152 100.00 100.00 2.82e-44 PDB 2Z59 "Complex Structures Of Mouse Rpn13 (22-130aa) And Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2ZCB "Crystal Structure Of Ubiquitin P37aP38A" 100.00 76 97.37 97.37 2.34e-44 PDB 2ZCC "Ubiquitin Crystallized Under High Pressure" 100.00 76 100.00 100.00 4.45e-46 PDB 2ZNV "Crystal Structure Of Human Amsh-Lp Dub Domain In Complex With Lys63-Linked Ubiquitin Dimer" 100.00 77 100.00 100.00 5.05e-46 PDB 2ZVN "Nemo Cozi Domain Incomplex With Diubiquitin In P212121 Space Group" 100.00 154 100.00 100.00 4.07e-45 PDB 2ZVO "Nemo Cozi Domain In Complex With Diubiquitin In C2 Space Group" 100.00 154 100.00 100.00 4.07e-45 PDB 3A1Q "Crystal Structure Of The Mouse Rap80 Uims In Complex With Lys63-Linked Di-Ubiquitin" 100.00 77 100.00 100.00 5.05e-46 PDB 3A33 "Ubch5b~ubiquitin Conjugate" 100.00 76 100.00 100.00 4.45e-46 PDB 3A9J "Crystal Structure Of The Mouse Tab2-Nzf In Complex With Lys63-Linked Di-Ubiquitin" 100.00 77 100.00 100.00 5.05e-46 PDB 3A9K "Crystal Structure Of The Mouse Tab3-Nzf In Complex With Lys63-Linked Di-Ubiquitin" 100.00 77 100.00 100.00 5.05e-46 PDB 3AI5 "Crystal Structure Of Yeast Enhanced Green Fluorescent Protein- Ubiquitin Fusion Protein" 97.37 307 100.00 100.00 1.12e-41 PDB 3ALB "Cyclic Lys48-Linked Tetraubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 3AUL "Crystal Structure Of Wild-Type Lys48-Linked Diubiquitin In An Open Conformation" 100.00 76 100.00 100.00 4.45e-46 PDB 3AXC "Crystal Structure Of Linear Diubiquitin" 100.00 154 100.00 100.00 4.07e-45 PDB 3B08 "Crystal Structure Of The Mouse Hoil1-l-nzf In Complex With Linear Di- Ubiquitin" 100.00 152 100.00 100.00 4.37e-45 PDB 3B0A "Crystal Structure Of The Mouse Hoil1-l-nzf In Complex With Linear Di- Ubiquitin" 100.00 152 100.00 100.00 4.37e-45 PDB 3BY4 "Structure Of Ovarian Tumor (Otu) Domain In Complex With Ubiquitin" 98.68 75 100.00 100.00 2.91e-45 PDB 3C0R "Structure Of Ovarian Tumor (Otu) Domain In Complex With Ubiquitin" 98.68 75 100.00 100.00 2.91e-45 PDB 3DVG "Crystal Structure Of K63-Specific Fab Apu.3a8 Bound To K63-Linked Di- Ubiquitin" 100.00 80 100.00 100.00 5.61e-46 PDB 3DVN "Crystal Structure Of K63-specific Fab Apu2.16 Bound To K63-linked Di- Ubiquitin" 100.00 80 100.00 100.00 5.61e-46 PDB 3EEC "X-Ray Structure Of Human Ubiquitin Cd(Ii) Adduct" 100.00 76 100.00 100.00 4.45e-46 PDB 3EFU "X-Ray Structure Of Human Ubiquitin-Hg(Ii) Adduct" 100.00 76 100.00 100.00 4.45e-46 PDB 3EHV "X-Ray Structure Of Human Ubiquitin Zn(Ii) Adduct" 100.00 76 100.00 100.00 4.45e-46 PDB 3H1U "Structure Of Ubiquitin In Complex With Cd Ions" 100.00 76 100.00 100.00 4.45e-46 PDB 3H7P "Crystal Structure Of K63-Linked Di-Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 3H7S "Crystal Structures Of K63-Linked Di- And Tri-Ubiquitin Reveal A Highly Extended Chain Architecture" 100.00 76 98.68 98.68 1.91e-43 PDB 3HM3 "The Structure And Conformation Of Lys-63 Linked Tetra-Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 3I3T "Crystal Structure Of Covalent Ubiquitin-usp21 Complex" 98.68 75 100.00 100.00 2.91e-45 PDB 3IFW "Crystal Structure Of The S18y Variant Of Ubiquitin Carboxy T Hydrolase L1 Bound To Ubiquitin Vinylmethylester." 98.68 75 100.00 100.00 2.91e-45 PDB 3IHP "Covalent Ubiquitin-Usp5 Complex" 98.68 75 100.00 100.00 2.91e-45 PDB 3JSV "Crystal Structure Of Mouse Nemo Cozi In Complex With Lys63- Linked Di-Ubiquitin" 100.00 77 100.00 100.00 5.05e-46 PDB 3JVZ E2~ubiquitin-Hect 100.00 81 100.00 100.00 5.23e-46 PDB 3JW0 E2~ubiquitin-Hect 100.00 81 100.00 100.00 5.23e-46 PDB 3K9O "The Crystal Structure Of E2-25k And Ubb+1 Complex" 98.68 96 100.00 100.00 8.68e-45 PDB 3K9P "The Crystal Structure Of E2-25k And Ubiquitin Complex" 100.00 79 100.00 100.00 4.53e-46 PDB 3KVF "Crystal Structure Of The I93m Mutant Of Ubiquitin Carboxy Te Hydrolase L1 Bound To Ubiquitin Vinylmethylester" 98.68 75 100.00 100.00 2.91e-45 PDB 3KW5 "Crystal Structure Of Ubiquitin Carboxy Terminal Hydrolase L1 Ubiquitin Vinylmethylester" 98.68 75 100.00 100.00 2.91e-45 PDB 3LDZ "Crystal Structure Of Human Stam1 Vhs Domain In Complex With Ubiquitin" 96.05 73 100.00 100.00 1.22e-43 PDB 3M3J "A New Crystal Form Of Lys48-Linked Diubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 3MHS "Structure Of The Saga Ubp8SGF11SUS1SGF73 DUB MODULE BOUND Ubiquitin Aldehyde" 98.68 76 100.00 100.00 2.99e-45 PDB 3MTN "Usp21 In Complex With A Ubiquitin-based, Usp21-specific Inhibitor" 88.16 85 98.51 98.51 1.00e-37 PDB 3N30 "Crystal Structure Of Cubic Zn3-Hub (Human Ubiquitin) Adduct" 100.00 76 100.00 100.00 4.45e-46 PDB 3N32 "The Crystal Structure Of Human Ubiquitin Adduct With Zeise's Salt" 100.00 76 100.00 100.00 4.45e-46 PDB 3NHE "High Resolution Structure (1.26a) Of Usp2a In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 3NOB "Structure Of K11-linked Di-ubiquitin" 100.00 78 100.00 100.00 3.66e-46 PDB 3NS8 "Crystal Structure Of An Open Conformation Of Lys48-Linked Diubiquitin At Ph 7.5" 100.00 76 100.00 100.00 4.45e-46 PDB 3O65 "Crystal Structure Of A Josephin-Ubiquitin Complex: Evolutionary Restraints On Ataxin-3 Deubiquitinating Activity" 98.68 76 100.00 100.00 2.99e-45 PDB 3OFI "Crystal Structure Of Human Insulin-Degrading Enzyme In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 3OJ3 "Crystal Structure Of The A20 Znf4 And Ubiquitin Complex" 100.00 79 100.00 100.00 4.53e-46 PDB 3OJ4 "Crystal Structure Of The A20 Znf4, Ubiquitin And Ubch5a Complex" 100.00 79 100.00 100.00 4.53e-46 PDB 3ONS "Crystal Structure Of Human Ubiquitin In A New Crystal Form" 94.74 72 100.00 100.00 5.80e-43 PDB 3PHD "Crystal Structure Of Human Hdac6 In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 3PHW "Otu Domain Of Crimean Congo Hemorrhagic Fever Virus In Complex With Ubiquitin" 98.68 75 100.00 100.00 2.91e-45 PDB 3PRM "Structural Analysis Of A Viral Otu Domain Protease From The Crimean- Congo Hemorrhagic Fever Virus In Complex With Human Ubiqui" 98.68 75 100.00 100.00 2.91e-45 PDB 3PRP "Structural Analysis Of A Viral Otu Domain Protease From The Crimean- Congo Hemorrhagic Fever Virus In Complex With Human Ubiqui" 98.68 75 100.00 100.00 2.91e-45 PDB 3PT2 "Structure Of A Viral Otu Domain Protease Bound To Ubiquitin" 98.68 75 100.00 100.00 2.91e-45 PDB 3PTF "X-Ray Structure Of The Non-Covalent Complex Between Ubch5a And Ubiquitin" 100.00 79 100.00 100.00 4.53e-46 PDB 3Q3F "Engineering Domain-Swapped Binding Interfaces By Mutually Exclusive Folding: Insertion Of Ubiquitin Into Position 103 Of Barnas" 98.68 189 100.00 100.00 3.64e-44 PDB 3RUL "New Strategy To Analyze Structures Of Glycopeptide-Target Complexes" 98.68 79 100.00 100.00 2.70e-45 PDB 3TBL "Structure Of Mono-ubiquitinated Pcna: Implications For Dna Polymerase Switching And Okazaki Fragment Maturation" 100.00 76 100.00 100.00 4.45e-46 PDB 3TMP "The Catalytic Domain Of Human Deubiquitinase Duba In Complex With Ubiquitin Aldehyde" 98.68 76 100.00 100.00 2.99e-45 PDB 3U30 "Crystal Structure Of A Linear-Specific Ubiquitin Fab Bound To Linear Ubiquitin" 100.00 172 100.00 100.00 4.69e-45 PDB 3UGB "Ubch5c~ubiquitin Conjugate" 100.00 76 100.00 100.00 4.45e-46 PDB 3VDZ "Tailoring Encodable Lanthanide-Binding Tags As Mri Contrast Agents: Xq-Dse3-Ubiquitin At 2.4 Angstroms" 100.00 111 100.00 100.00 9.99e-46 PDB 3VFK "The Structure Of Monodechloro-teicoplanin In Complex With Its Ligand, Using Ubiquitin As A Ligand Carrier" 98.68 79 100.00 100.00 2.70e-45 PDB 3VHT "Crystal Structure Of Gfp-Wrnip1 Ubz Domain Fusion Protein In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 3VUW "Crystal Structure Of A20 Zf7 In Complex With Linear Ubiquitin, Form I" 100.00 76 100.00 100.00 4.45e-46 PDB 3VUX "Crystal Structure Of A20 Zf7 In Complex With Linear Ubiquitin, Form Ii" 100.00 76 100.00 100.00 4.45e-46 PDB 3VUY "Crystal Structure Of A20 Zf7 In Complex With Linear Tetraubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 3ZLZ "Lys6-linked Tri-ubiquitin" 100.00 76 98.68 100.00 1.06e-45 PDB 3ZNH "Crimean Congo Hemorrhagic Fever Virus Otu Domain In Complex With Ubiquitin-propargyl." 98.68 76 100.00 100.00 2.99e-45 PDB 3ZNI "Structure Of Phosphotyr363-cbl-b - Ubch5b-ub - Zap-70 Peptide Complex" 100.00 81 100.00 100.00 5.23e-46 PDB 3ZNZ "Crystal Structure Of Otulin Otu Domain (c129a) In Complex With Met1-di Ubiquitin" 100.00 152 100.00 100.00 4.37e-45 PDB 4A18 "T.Thermophila 60s Ribosomal Subunit In Complex With Initiation Factor 6. This File Contains 26s Rrna And Proteins Of Molecule 1" 100.00 129 97.37 98.68 1.83e-44 PDB 4A19 "T.Thermophila 60s Ribosomal Subunit In Complex With Initiation Factor 6. This File Contains 26s Rrna And Proteins Of Molecule 2" 100.00 129 97.37 98.68 1.83e-44 PDB 4A1B "T.Thermophila 60s Ribosomal Subunit In Complex With Initiation Factor 6. This File Contains 26s Rrna And Proteins Of Molecule 3" 100.00 129 97.37 98.68 1.83e-44 PDB 4A1D "T.Thermophila 60s Ribosomal Subunit In Complex With Initiation Factor 6. This File Contains 26s Rrna And Proteins Of Molecule 4" 100.00 129 97.37 98.68 1.83e-44 PDB 4ADX "The Cryo-em Structure Of The Archaeal 50s Ribosomal Subunit In Complex With Initiation Factor 6" 100.00 129 97.37 98.68 1.83e-44 PDB 4AP4 "Rnf4 - Ubch5a - Ubiquitin Heterotrimeric Complex" 100.00 80 100.00 100.00 4.66e-46 PDB 4AUQ "Structure Of Birc7-Ubch5b-Ub Complex." 100.00 81 100.00 100.00 5.23e-46 PDB 4BBN "Nedd4 Hect-ub:ub Complex" 100.00 76 100.00 100.00 4.45e-46 PDB 4BOS "Structure Of Otud2 Otu Domain In Complex With Ubiquitin K11- Linked Peptide" 100.00 76 100.00 100.00 4.45e-46 PDB 4BOZ "Structure Of Otud2 Otu Domain In Complex With K11-linked Di Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 4BVU "Structure Of Shigella Effector Ospg In Complex With Host Ubch5c-ubiquitin Conjugate" 100.00 76 100.00 100.00 4.45e-46 PDB 4CXC "Regulation Of The Mammalian Elongation Cycle By 40s Subunit Rolling: A Eukaryotic-specific Ribosome Rearrangement" 100.00 156 100.00 100.00 5.21e-46 PDB 4CXD "Regulation Of The Mammalian Elongation Cycle By 40s Subunit Rolling: A Eukaryotic-specific Ribosome Rearrangement" 100.00 128 100.00 100.00 3.09e-46 PDB 4DDG "Crystal Structure Of Human Otub1UBCH5B~UBUB" 100.00 76 100.00 100.00 4.45e-46 PDB 4DDI "Crystal Structure Of Human Otub1UBCH5B~UBUB" 100.00 76 100.00 100.00 4.45e-46 PDB 4DHJ "The Structure Of A Ceotub1 Ubiquitin Aldehyde Ubc13~ub Complex" 98.68 76 100.00 100.00 2.71e-45 PDB 4DHZ "The Structure Of HCEOTUB1-Ubiquitin Aldehyde-Ubc13~ub" 98.68 76 100.00 100.00 2.71e-45 PDB 4FJV "Crystal Structure Of Human Otubain2 And Ubiquitin Complex" 100.00 86 100.00 100.00 6.71e-46 PDB 4HXD "Diversity Of Ubiquitin And Isg15 Specificity Amongst Nairoviruses Viral Ovarian Tumor Domain Proteases" 98.68 75 100.00 100.00 2.91e-45 PDB 4I6N "Crystal Structure Of Trichinella Spiralis Uch37 Catalytic Domain Bound To Ubiquitin Vinyl Methyl Ester" 97.37 75 100.00 100.00 3.09e-44 PDB 4IG7 "Crystal Structure Of Trichinella Spiralis Uch37 Bound To Ubiquitin Vinyl Methyl Ester" 98.68 75 100.00 100.00 2.91e-45 PDB 4IUM "Equine Arteritis Virus Papain-like Protease 2 (plp2) Covalently Bound To Ubiquitin" 98.68 76 100.00 100.00 2.99e-45 PDB 4JIO "Bro1 V Domain And Ubiquitin" 100.00 76 98.68 98.68 3.30e-45 PDB 4JQW "Crystal Structure Of A Complex Of Nod1 Card And Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 4K1R "Crystal Structure Of Schizosaccharomyces Pombe Sst2 Catalytic Domain And Ubiquitin" 100.00 81 100.00 100.00 7.82e-46 PDB 4K7S "Crystal Structure Of Zn2-hub (human Ubiquitin) Adduct From A Solution 35 Mm Zinc Acetate/1.3 Mm Hub" 100.00 76 100.00 100.00 4.45e-46 PDB 4K7U "Crystal Structure Of Zn2.3-hub (human Ubiquitin) Adduct From A Solution 70 Mm Zinc Acetate/1.3 Mm Hub" 100.00 76 100.00 100.00 4.45e-46 PDB 4K7W "Crystal Structure Of Zn3-hub(human Ubiquitin) Adduct From A Solution 100 Mm Zinc Acetate/1.3 Mm Hub" 100.00 76 100.00 100.00 4.45e-46 PDB 4KSK "Gumby/fam105b In Complex With Ubiquitin" 100.00 80 100.00 100.00 4.66e-46 PDB 4KSL "Gumby/fam105b In Complex With Linear Di-ubiquitin" 100.00 156 100.00 100.00 4.31e-45 PDB 4KZX "Rabbit 40s Ribosomal Subunit In Complex With Eif1." 100.00 156 100.00 100.00 5.21e-46 PDB 4KZY "Rabbit 40s Ribosomal Subunit In Complex With Eif1 And Eif1a." 100.00 156 100.00 100.00 5.21e-46 PDB 4KZZ "Rabbit 40s Ribosomal Subunit In Complex With Mrna, Initiator Trna And Eif1a" 100.00 156 100.00 100.00 5.21e-46 PDB 4LCD "Structure Of An Rsp5xubxsna3 Complex: Mechanism Of Ubiquitin Ligation And Lysine Prioritization By A Hect E3" 97.37 83 100.00 100.00 1.22e-44 PDB 4LDT "The Structure Of H/ceotub1-ubiquitin Aldehyde-ubch5b~ub" 100.00 76 100.00 100.00 4.45e-46 PDB 4LJO "Structure Of An Active Ligase (hoip)/ubiquitin Transfer Complex" 100.00 76 100.00 100.00 4.45e-46 PDB 4LJP "Structure Of An Active Ligase (hoip-h889a)/ubiquitin Transfer Complex" 100.00 76 100.00 100.00 4.45e-46 PDB 4M0W "Crystal Structure Of Sars-cov Papain-like Protease C112s Mutant In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 4MDK "Cdc34-ubiquitin-cc0651 Complex" 100.00 80 100.00 100.00 4.66e-46 PDB 4MM3 "Crystal Structure Of Sars-cov Papain-like Protease Plpro In Complex With Ubiquitin Aldehyde" 98.68 76 100.00 100.00 2.99e-45 PDB 4MSM "Crystal Structure Of Schizosaccharomyces Pombe Amsh-like Protease Sst2 E286a Mutant Bound To Ubiquitin" 100.00 81 100.00 100.00 7.82e-46 PDB 4MSQ "Crystal Structure Of Schizosaccharomyces Pombe Amsh-like Protease Sst2 Catalytic Domain Bound To Ubiquitin" 100.00 81 100.00 100.00 7.82e-46 PDB 4NQK "Structure Of An Ubiquitin Complex" 100.00 79 100.00 100.00 8.16e-46 PDB 4NQL "The Crystal Structure Of The Dub Domain Of Amsh Orthologue, Sst2 From S. Pombe, In Complex With Lysine 63-linked Diubiquitin" 100.00 77 100.00 100.00 5.05e-46 PDB 4P4H "Caught-in-action Signaling Complex Of Rig-i 2card Domain And Mavs Card Domain" 100.00 79 100.00 100.00 8.16e-46 PDB 4PIG "Crystal Structure Of The Ubiquitin K11s Mutant" 100.00 76 98.68 98.68 1.92e-45 PDB 4PIH "X-ray Crystal Structure Of The K33s Mutant Of Ubiquitin" 100.00 76 98.68 98.68 1.92e-45 PDB 4PIJ "X-ray Crystal Structure Of The K11s/k63s Double Mutant Of Ubiquitin" 98.68 75 97.33 97.33 5.38e-44 PDB 4PQT "Insights Into The Mechanism Of Deubiquitination By Jamm Deubiquitinases From Co-crystal Structures Of Enzyme With Substrate And" 100.00 81 100.00 100.00 7.82e-46 PDB 4RF0 "Crystal Structure Of The Middle-east Respiratory Syndrome Coronavirus Papain-like Protease In Complex With Ubiquitin (space Gro" 98.68 75 100.00 100.00 2.91e-45 PDB 4RF1 "Crystal Structure Of The Middle-east Respiratory Syndrome Coronavirus Papain-like Protease In Complex With Ubiquitin (space Gro" 98.68 75 100.00 100.00 2.91e-45 PDB 4UN2 "Crystal Structure Of The Uba Domain Of Dsk2 In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 4UPX "Mammalian 80s Hcv-ires Initiation Complex With Eif5b Pre-like State" 100.00 128 100.00 100.00 3.09e-46 PDB 4UQ1 "Mammalian 80s Hcv-ires Initiation Complex With Eif5b Post-like State" 100.00 128 100.00 100.00 3.09e-46 PDB 4UQ4 "Mammalian 80s Hcv-ires Initiation Complex With Eif5b Post-like State" 100.00 156 100.00 100.00 5.21e-46 PDB 4UQ5 "Mammalian 80s Hcv-ires Initiation Complex With Eif5b Pre-like State" 100.00 156 100.00 100.00 5.21e-46 PDB 4W20 "Structure Of The Mammalian 60s Ribosomal Subunit (this Entry Contains The Large Ribosomal Proteins)" 100.00 128 100.00 100.00 3.09e-46 PDB 4W22 "Structure Of The 80s Mammalian Ribosome Bound To Eef2 (this Entry Contains The Large Ribosomal Subunit Proteins)" 100.00 128 100.00 100.00 3.09e-46 PDB 4W23 "Structure Of The 80s Mammalian Ribosome Bound To Eef2 (this Entry Contains The Small Ribosomal Subunit)" 100.00 156 100.00 100.00 5.21e-46 PDB 4W25 "Structure Of The Idle Mammalian Ribosome-sec61 Complex (this Entry Contains The Large Ribosomal Subunit Proteins)" 100.00 128 100.00 100.00 3.09e-46 PDB 4W27 "Structure Of The Translating Mammalian Ribosome-sec61 Complex (this Entry Contains The Large Ribosomal Subunit Proteins)" 100.00 128 100.00 100.00 3.09e-46 PDB 4W28 "Structure Of The Translating Mammalian Ribosome-sec61 Complex (this Entry Contains The Small Ribosomal Subunit)" 100.00 156 100.00 100.00 5.21e-46 PDB 4WUR "The Crystal Structure Of The Mers-cov Papain-like Protease (c111s) With Human Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 4WZP "Ser65 Phosphorylated Ubiquitin, Major Conformation" 100.00 76 98.68 98.68 3.16e-45 DBJ BAA03983 "polyubiquitin [Rattus norvegicus]" 100.00 305 100.00 100.00 2.21e-43 DBJ BAA09860 "polyubiquitin [Homo sapiens]" 100.00 611 98.68 98.68 1.04e-40 DBJ BAA11842 "ubiquitin [Cavia porcellus]" 100.00 311 100.00 100.00 2.40e-43 DBJ BAA11843 "ubiquitin extention protein [Cavia porcellus]" 100.00 156 100.00 100.00 5.21e-46 DBJ BAA23486 "polyubiquitin [Homo sapiens]" 100.00 609 98.68 98.68 5.60e-41 EMBL CAA25706 "unnamed protein product [Saccharomyces cerevisiae]" 50.00 191 100.00 100.00 2.98e-16 EMBL CAA26488 "unnamed protein product [Gallus gallus]" 100.00 157 98.68 98.68 2.66e-44 EMBL CAA28495 "ubiquitin [Homo sapiens]" 100.00 229 100.00 100.00 3.53e-44 EMBL CAA30183 "unnamed protein product [Dictyostelium discoideum]" 100.00 128 97.37 97.37 5.38e-44 EMBL CAA30815 "unnamed protein product [Cricetulus sp.]" 93.42 223 100.00 100.00 3.70e-40 GB AAA02769 "polyprotein [Bovine viral diarrhea virus 1-Osloss]" 98.68 3975 97.33 100.00 1.83e-39 GB AAA28154 "polyubiquitin [Caenorhabditis elegans]" 100.00 838 97.37 98.68 7.19e-40 GB AAA28997 "ubiquitin [Drosophila melanogaster]" 100.00 231 100.00 100.00 3.30e-44 GB AAA28998 "ubiquitin-hybrid protein precursor [Drosophila melanogaster]" 100.00 156 100.00 100.00 9.66e-46 GB AAA28999 "ubiquitin, partial [Drosophila melanogaster]" 100.00 76 100.00 100.00 4.45e-46 PIR I50437 "polyubiquitin 4 - chicken [Gallus gallus]" 100.00 305 100.00 100.00 2.21e-43 PIR I51568 "polyubiquitin - African clawed frog (fragment)" 100.00 167 100.00 100.00 6.83e-45 PIR I65237 "ubiquitin / ribosomal protein L40, cytosolic [validated] - rat" 100.00 128 100.00 100.00 3.09e-46 PIR JN0790 "ubiquitin/ribosomal protein CEP52 fusion protein - Leishmania major" 100.00 128 97.37 98.68 2.33e-45 PIR S13928 "ubiquitin precursor - chicken [Gallus gallus]" 100.00 229 100.00 100.00 3.64e-44 PRF 0412265A ubiquitin 98.68 75 98.67 98.67 1.22e-44 PRF 1101405A "ubiquitin precursor" 50.00 191 100.00 100.00 2.95e-16 PRF 1212243A "ubiquitin S1" 100.00 76 100.00 100.00 4.45e-46 PRF 1212243B "ubiquitin S5" 92.11 77 98.57 98.57 7.61e-41 PRF 1212243C "ubiquitin S3" 100.00 76 100.00 100.00 4.45e-46 REF NP_001005123 "ubiquitin A-52 residue ribosomal protein fusion product 1 [Xenopus (Silurana) tropicalis]" 100.00 128 100.00 100.00 3.09e-46 REF NP_001006688 "ubiquitin C [Xenopus (Silurana) tropicalis]" 100.00 609 100.00 100.00 9.87e-42 REF NP_001009117 "polyubiquitin-B [Pan troglodytes]" 100.00 229 100.00 100.00 3.53e-44 REF NP_001009202 "polyubiquitin-B [Ovis aries]" 100.00 305 98.68 100.00 3.89e-43 REF NP_001009286 "ubiquitin-60S ribosomal protein L40 [Ovis aries]" 100.00 128 100.00 100.00 3.09e-46 SP P0C273 "RecName: Full=Ubiquitin-60S ribosomal protein L40; AltName: Full=Ubiquitin A-52 residue ribosomal protein fusion product 1; Con" 100.00 128 100.00 100.00 3.09e-46 SP P0C275 "RecName: Full=Ubiquitin-60S ribosomal protein L40; AltName: Full=Ubiquitin A-52 residue ribosomal protein fusion product 1; Con" 100.00 128 100.00 100.00 3.09e-46 SP P0C276 "RecName: Full=Ubiquitin-60S ribosomal protein L40; AltName: Full=Ubiquitin A-52 residue ribosomal protein fusion product 1; Con" 100.00 128 100.00 100.00 3.09e-46 SP P0CG47 "RecName: Full=Polyubiquitin-B; Contains: RecName: Full=Ubiquitin; Flags: Precursor [Homo sapiens]" 100.00 229 100.00 100.00 3.53e-44 SP P0CG48 "RecName: Full=Polyubiquitin-C; Contains: RecName: Full=Ubiquitin; Flags: Precursor [Homo sapiens]" 100.00 685 100.00 100.00 1.45e-41 TPD FAA00319 "TPA: polyubiquitin [Cryptococcus neoformans var. neoformans B-3501A]" 100.00 456 97.37 98.68 7.53e-41 TPG DAA18802 "TPA: polyubiquitin [Bos taurus]" 100.00 305 100.00 100.00 2.33e-43 TPG DAA20663 "TPA: ubiquitin C [Bos taurus]" 98.68 314 98.67 100.00 8.85e-42 TPG DAA20672 "TPA: ubiquitin B-like [Bos taurus]" 100.00 77 98.68 98.68 3.32e-45 TPG DAA24675 "TPA: 40S ribosomal protein S27a [Bos taurus]" 100.00 156 100.00 100.00 5.21e-46 TPG DAA28295 "TPA: ubiquitin and ribosomal protein L40 [Bos taurus]" 100.00 128 100.00 100.00 3.09e-46 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $SH3A 'house mouse' 10090 Eukaryota Metazoa Mus musculus $Ubiquitin human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $SH3A 'recombinant technology' . Escherichia coli . pET21a $Ubiquitin 'recombinant technology' . Escherichia coli . pET29b stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details '0.2 mM [U-99% 15N] CD2AP SH3-A, 1 mM UBIQUITIN, 90% H2O/10% D2O' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $SH3A 0.2 mM '[U-99% 15N]' $Ubiquitin 1 mM 'natural abundance' H2O 90 % 'natural abundance' D2O 10 % '[U-100% 2H]' stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type solution _Details '0.25 mM [U-99% 15N] UBIQUITIN, mM CD2AP SH3-A, 90% H2O/10% D2O' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $SH3A . mM 'natural abundance' $Ubiquitin 0.25 mM '[U-99% 15N]' H2O 90 % 'natural abundance' D2O 10 % '[U-100% 2H]' stop_ save_ save_sample_3 _Saveframe_category sample _Sample_type solution _Details '0.9 mM [U-99% 13CU-99% 15N] CD2AP SH3-A, 0.46 mM [U-99% 15N] UBIQUITIN,' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $SH3A 0.9 mM '[U-99% 13C; U-99% 15N]' $Ubiquitin 0.46 mM '[U-99% 15N]' H2O 90 % 'natural abundance' D2O 10 % '[U-100% 2H]' stop_ save_ save_sample_4 _Saveframe_category sample _Sample_type solution _Details '0.8 mM [U-99% 13CU-99% 15N] CD2AP SH3-A, 1.37 mM [U-99% 15N] UBIQUITIN,' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $SH3A 0.8 mM '[U-99% 13C; U-99% 15N]' $Ubiquitin 1.37 mM '[U-99% 15N]' H2O 90 % 'natural abundance' D2O 10 % '[U-100% 2H]' stop_ save_ save_sample_5 _Saveframe_category sample _Sample_type solution _Details '0.7 mM [U-99% 13CU-99% 15N] CD2AP SH3-A, 1.75 mM [U-99% 15N] UBIQUITIN,' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $SH3A 0.7 mM '[U-99% 13C; U-99% 15N]' $Ubiquitin 1.75 mM '[U-99% 15N]' H2O 90 % 'natural abundance' D2O 10 % '[U-100% 2H]' stop_ save_ save_sample_6 _Saveframe_category sample _Sample_type solution _Details '0.25 mM [U-99% 13C; U-99% 15N] CD2AP SH3-A, 1.75 MM [U-99% 15N] UBIQUITIN,' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $SH3A 0.25 mM '[U-99% 13C; U-99% 15N]' $Ubiquitin 1.75 mM '[U-99% 15N]' H2O 90 % 'natural abundance' D2O 10 % '[U-100% 2H]' stop_ save_ ############################ # Computer software used # ############################ save_HADDOCK _Saveframe_category software _Name HADDOCK _Version CNS/SCULPTOR loop_ _Vendor _Address _Electronic_address 'DOMINGUEZ, BOELENS' . . stop_ loop_ _Task refinement stop_ _Details . save_ save_CNS-SCULPTOR _Saveframe_category software _Name CNS/SCULPTOR _Version . loop_ _Vendor _Address _Electronic_address 'Brunger, Adams, Clore, Gros, Nilges and Read' . . stop_ loop_ _Task 'structure solution' stop_ _Details . save_ save_NMRVIEW _Saveframe_category software _Name NMRView _Version . loop_ _Vendor _Address _Electronic_address 'Johnson, One Moon Scientific' . . stop_ loop_ _Task 'structure solution' stop_ _Details . save_ save_HADDOCK1 _Saveframe_category software _Name HADDOCK _Version . loop_ _Vendor _Address _Electronic_address 'Brunger, Adams, Clore, Gros, Nilges and Read' . . stop_ loop_ _Task 'structure solution' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer VARIAN _Model 'UNIFORM NMR SYSTEM' _Field_strength 600 _Details . save_ save_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model 'Uniform NMR System' _Field_strength 800 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_HNCO_JNH,_JCC,_JCH,_JCAHA_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO JNH, JCC, JCH, JCAHA' _Sample_label $sample_3 save_ save_3D_HNCO_JNH,_JCC,_JCH,_JCAHA_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO JNH, JCC, JCH, JCAHA' _Sample_label $sample_4 save_ save_3D_HNCO_JNH,_JCC,_JCH,_JCAHA_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO JNH, JCC, JCH, JCAHA' _Sample_label $sample_5 save_ save_2D_1H-15N_HSQC_FILTERED_DIPSAP_5 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC FILTERED DIPSAP' _Sample_label $sample_6 save_ save_2D_1H-15N_HSQC_6 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_2 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0 . mM pH 6.0 . pH pressure 1 . atm temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio water H 1 protons ppm 4.773 internal direct . . . 1.000000000 water N 15 protons ppm 117.460 internal indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_CD2AP_SH3-A _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chem_shift_reference _Mol_system_component_name SH3A _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 2 1 VAL H H 7.1985 . 1 2 2 1 VAL N N 104.2422 . 1 3 3 2 ASP H H 9.2149 . 1 4 3 2 ASP N N 128.6060 . 1 5 4 3 TYR H H 9.3242 . 1 6 4 3 TYR N N 120.1495 . 1 7 5 4 ILE H H 9.4176 . 1 8 5 4 ILE N N 119.2504 . 1 9 6 5 VAL H H 8.5314 . 1 10 6 5 VAL N N 126.1500 . 1 11 7 6 GLU H H 9.2403 . 1 12 7 6 GLU N N 129.2890 . 1 13 8 7 TYR H H 7.7246 . 1 14 8 7 TYR N N 117.5000 . 1 15 9 8 ASP H H 8.0263 . 1 16 9 8 ASP N N 118.9636 . 1 17 10 9 TYR H H 8.1342 . 1 18 10 9 TYR N N 118.3098 . 1 19 11 10 ASP H H 7.8067 . 1 20 11 10 ASP N N 128.4192 . 1 21 12 11 ALA H H 7.7854 . 1 22 12 11 ALA N N 123.0842 . 1 23 13 12 VAL H H 8.5167 . 1 24 13 12 VAL N N 118.5913 . 1 25 14 13 HIS H H 7.0489 . 1 26 14 13 HIS N N 115.1454 . 1 27 15 14 ASP H H 8.8229 . 1 28 15 14 ASP N N 120.6948 . 1 29 16 15 ASP H H 8.4443 . 1 30 16 15 ASP N N 115.8856 . 1 31 17 16 GLU H H 7.7616 . 1 32 17 16 GLU N N 119.6430 . 1 33 18 17 LEU H H 8.9244 . 1 34 18 17 LEU N N 120.6865 . 1 35 19 18 THR H H 7.4620 . 1 36 19 18 THR N N 117.2276 . 1 37 20 19 ILE H H 9.2689 . 1 38 20 19 ILE N N 121.4752 . 1 39 21 20 ARG H H 8.8093 . 1 40 21 20 ARG N N 124.9405 . 1 41 22 21 VAL H H 7.9940 . 1 42 22 21 VAL N N 118.2997 . 1 43 23 22 GLY H H 8.6898 . 1 44 23 22 GLY N N 114.5722 . 1 45 24 23 GLU H H 8.0757 . 1 46 24 23 GLU N N 120.0048 . 1 47 25 24 ILE H H 7.9721 . 1 48 25 24 ILE N N 119.3760 . 1 49 26 25 ILE H H 9.3210 . 1 50 26 25 ILE N N 128.3572 . 1 51 27 26 ARG H H 8.6547 . 1 52 27 26 ARG N N 123.6202 . 1 53 28 27 ASN H H 8.6079 . 1 54 28 27 ASN N N 117.4519 . 1 55 29 28 VAL H H 8.6610 . 1 56 29 28 VAL N N 120.5800 . 1 57 30 29 LYS H H 9.3840 . 1 58 30 29 LYS N N 127.3724 . 1 59 31 30 LYS H H 8.4928 . 1 60 31 30 LYS N N 121.8100 . 1 61 32 31 LEU H H 7.6534 . 1 62 32 31 LEU N N 123.3408 . 1 63 33 32 GLN H H 8.4348 . 1 64 33 32 GLN N N 118.3678 . 1 65 34 33 GLU H H 7.3361 . 1 66 34 33 GLU N N 119.3453 . 1 67 35 34 GLU H H 8.3521 . 1 68 35 34 GLU N N 126.2513 . 1 69 36 35 GLY H H 8.5104 . 1 70 36 35 GLY N N 111.3438 . 1 71 37 36 TRP H H 7.7272 . 1 72 37 36 TRP N N 119.2001 . 1 73 38 37 LEU H H 8.4018 . 1 74 38 37 LEU N N 123.0249 . 1 75 39 38 GLU H H 8.4573 . 1 76 39 38 GLU N N 117.5759 . 1 77 40 39 GLY H H 8.7652 . 1 78 40 39 GLY N N 110.7932 . 1 79 41 40 GLU H H 9.0394 . 1 80 41 40 GLU N N 118.9580 . 1 81 42 41 LEU H H 9.0324 . 1 82 42 41 LEU N N 127.6811 . 1 83 43 42 ASN H H 9.5823 . 1 84 43 42 ASN N N 126.6870 . 1 85 44 43 GLY H H 8.9839 . 1 86 44 43 GLY N N 104.0386 . 1 87 45 44 ARG H H 7.7144 . 1 88 45 44 ARG N N 120.1037 . 1 89 46 45 ARG H H 8.5652 . 1 90 46 45 ARG N N 121.8399 . 1 91 47 46 GLY H H 8.6619 . 1 92 47 46 GLY N N 109.6915 . 1 93 48 47 MET H H 8.8615 . 1 94 48 47 MET N N 117.1998 . 1 95 49 48 PHE H H 8.8294 . 1 96 49 48 PHE N N 116.7175 . 1 97 51 50 ASP H H 8.1756 . 1 98 51 50 ASP N N 120.4852 . 1 99 52 51 ASN H H 8.7211 . 1 100 52 51 ASN N N 110.0397 . 1 101 53 52 PHE H H 7.5357 . 1 102 53 52 PHE N N 117.9612 . 1 103 54 53 VAL H H 7.4643 . 1 104 54 53 VAL N N 109.9805 . 1 105 55 54 LYS H H 9.1562 . 1 106 55 54 LYS N N 120.3570 . 1 107 56 55 GLU H H 8.6768 . 1 108 56 55 GLU N N 128.6891 . 1 109 57 56 ILE H H 8.4395 . 1 110 57 56 ILE N N 127.1399 . 1 111 58 57 LYS H H 8.3463 . 1 112 58 57 LYS N N 128.7337 . 1 113 59 58 ARG H H 8.5355 . 1 114 59 58 ARG N N 123.6777 . 1 115 60 59 GLU H H 8.6789 . 1 116 60 59 GLU N N 122.6065 . 1 117 61 60 THR H H 8.0855 . 1 118 61 60 THR N N 114.0068 . 1 119 62 61 GLU H H 8.0214 . 1 120 62 61 GLU N N 128.0030 . 1 stop_ save_ save_Ubiquitin-CS _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_2 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chem_shift_reference _Mol_system_component_name Ubiquitin _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 2 2 GLN H H 8.9406 . 1 2 2 2 GLN N N 122.7356 . 1 3 3 3 ILE H H 8.2877 . 1 4 3 3 ILE N N 114.9128 . 1 5 4 4 PHE H H 8.6037 . 1 6 4 4 PHE N N 119.0784 . 1 7 5 5 VAL H H 9.2264 . 1 8 5 5 VAL N N 120.9824 . 1 9 6 6 LYS H H 8.7626 . 1 10 6 6 LYS N N 127.9308 . 1 11 7 7 THR H H 8.8171 . 1 12 7 7 THR N N 116.7931 . 1 13 8 8 LEU H H 8.8958 . 1 14 8 8 LEU N N 121.0708 . 1 15 9 9 THR H H 7.7265 . 1 16 9 9 THR N N 106.1762 . 1 17 10 10 GLY H H 7.8418 . 1 18 10 10 GLY N N 110.0909 . 1 19 11 11 LYS H H 7.2077 . 1 20 11 11 LYS N N 121.6026 . 1 21 12 12 THR H H 8.6550 . 1 22 12 12 THR N N 120.6988 . 1 23 13 13 ILE H H 9.4433 . 1 24 13 13 ILE N N 126.8434 . 1 25 14 14 THR H H 8.7054 . 1 26 14 14 THR N N 121.2559 . 1 27 15 15 LEU H H 8.7480 . 1 28 15 15 LEU N N 125.3824 . 1 29 16 16 GLU H H 8.1422 . 1 30 16 16 GLU N N 122.6980 . 1 31 17 17 VAL H H 8.9398 . 1 32 17 17 VAL N N 117.8273 . 1 33 18 18 GLU H H 8.6633 . 1 34 18 18 GLU N N 119.4972 . 1 35 20 20 SER H H 7.0325 . 1 36 20 20 SER N N 103.5587 . 1 37 21 21 ASP H H 8.0019 . 1 38 21 21 ASP N N 123.6872 . 1 39 22 22 THR H H 7.9010 . 1 40 22 22 THR N N 109.1640 . 1 41 23 23 ILE H H 8.5541 . 1 42 23 23 ILE N N 121.5496 . 1 43 24 24 GLU H H 9.7612 . 1 44 24 24 GLU N N 121.3562 . 1 45 25 25 ASN H H 7.9271 . 1 46 25 25 ASN N N 121.4736 . 1 47 26 26 VAL H H 8.1056 . 1 48 26 26 VAL N N 122.2827 . 1 49 27 27 LYS H H 8.5966 . 1 50 27 27 LYS N N 119.0579 . 1 51 28 28 ALA H H 8.0587 . 1 52 28 28 ALA N N 124.0684 . 1 53 29 29 LYS H H 7.8509 . 1 54 29 29 LYS N N 120.3390 . 1 55 30 30 ILE H H 8.2675 . 1 56 30 30 ILE N N 121.3845 . 1 57 31 31 GLN H H 8.5770 . 1 58 31 31 GLN N N 123.7750 . 1 59 32 32 ASP H H 8.0106 . 1 60 32 32 ASP N N 119.8750 . 1 61 33 33 LYS H H 7.4134 . 1 62 33 33 LYS N N 115.6012 . 1 63 34 34 GLU H H 8.7332 . 1 64 34 34 GLU N N 114.3947 . 1 65 35 35 GLY H H 8.5128 . 1 66 35 35 GLY N N 108.9926 . 1 67 36 36 ILE H H 6.1512 . 1 68 36 36 ILE N N 120.4212 . 1 69 39 39 ASP H H 8.5464 . 1 70 39 39 ASP N N 113.6537 . 1 71 40 40 GLN H H 7.8016 . 1 72 40 40 GLN N N 116.9316 . 1 73 41 41 GLN H H 7.4604 . 1 74 41 41 GLN N N 118.0586 . 1 75 42 42 ARG H H 8.4036 . 1 76 42 42 ARG N N 122.7206 . 1 77 43 43 LEU H H 8.8891 . 1 78 43 43 LEU N N 124.5336 . 1 79 44 44 ILE H H 9.2342 . 1 80 44 44 ILE N N 122.9075 . 1 81 45 45 PHE H H 8.9617 . 1 82 45 45 PHE N N 126.4654 . 1 83 46 46 ALA H H 8.9254 . 1 84 46 46 ALA N N 133.3630 . 1 85 47 47 GLY H H 8.3916 . 1 86 47 47 GLY N N 103.1330 . 1 87 48 48 LYS H H 7.8460 . 1 88 48 48 LYS N N 121.3570 . 1 89 49 49 GLN H H 8.6081 . 1 90 49 49 GLN N N 121.9214 . 1 91 50 50 LEU H H 8.6235 . 1 92 50 50 LEU N N 125.9136 . 1 93 51 51 GLU H H 8.4386 . 1 94 51 51 GLU N N 123.3652 . 1 95 52 52 ASP H H 8.1692 . 1 96 52 52 ASP N N 120.7119 . 1 97 53 53 GLY H H 8.2322 . 1 98 53 53 GLY N N 108.5236 . 1 99 54 54 ARG H H 7.4811 . 1 100 54 54 ARG N N 119.5451 . 1 101 55 55 THR H H 8.8399 . 1 102 55 55 THR N N 108.9745 . 1 103 56 56 LEU H H 8.1351 . 1 104 56 56 LEU N N 118.2391 . 1 105 57 57 SER H H 8.4942 . 1 106 57 57 SER N N 113.6076 . 1 107 58 58 ASP H H 7.9472 . 1 108 58 58 ASP N N 124.7085 . 1 109 59 59 TYR H H 7.2603 . 1 110 59 59 TYR N N 115.9306 . 1 111 60 60 ASN H H 8.1617 . 1 112 60 60 ASN N N 116.0464 . 1 113 61 61 ILE H H 7.2489 . 1 114 61 61 ILE N N 119.0773 . 1 115 62 62 GLN H H 7.6501 . 1 116 62 62 GLN N N 125.0868 . 1 117 63 63 LYS H H 8.4949 . 1 118 63 63 LYS N N 120.6913 . 1 119 64 64 GLU H H 9.3047 . 1 120 64 64 GLU N N 114.7634 . 1 121 65 65 SER H H 7.6469 . 1 122 65 65 SER N N 114.9971 . 1 123 66 66 THR H H 8.7434 . 1 124 66 66 THR N N 117.8919 . 1 125 67 67 LEU H H 9.2992 . 1 126 67 67 LEU N N 127.2772 . 1 127 68 68 HIS H H 8.9807 . 1 128 68 68 HIS N N 118.4695 . 1 129 69 69 LEU H H 8.1310 . 1 130 69 69 LEU N N 123.5272 . 1 131 70 70 VAL H H 9.1458 . 1 132 70 70 VAL N N 125.2070 . 1 133 71 71 LEU H H 7.9182 . 1 134 71 71 LEU N N 123.3845 . 1 135 72 72 ARG H H 8.7248 . 1 136 72 72 ARG N N 123.7981 . 1 137 73 73 LEU H H 8.7052 . 1 138 73 73 LEU N N 125.2946 . 1 139 74 74 ARG H H 8.5483 . 1 140 74 74 ARG N N 122.5154 . 1 141 75 75 GLY H H 8.5294 . 1 142 75 75 GLY N N 110.4934 . 1 143 76 76 GLY H H 8.1557 . 1 144 76 76 GLY N N 115.1597 . 1 stop_ save_