data_19478 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Backbone assignments for TNRC6B motif I (599-683) ; _BMRB_accession_number 19478 _BMRB_flat_file_name bmr19478.str _Entry_type original _Submission_date 2013-09-06 _Accession_date 2013-09-06 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details 'The backbone assignments (H,N,CA,CB) for the human GW-protein TNRC6B motif I, residues 599-683.' loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Hennig Janosch . . 2 Sattler Michael . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 73 "13C chemical shifts" 139 "15N chemical shifts" 73 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2013-02-03 update BMRB 'update entry citation' 2010-10-04 original author 'original release' stop_ save_ ############################# # Citation for this entry # ############################# save_Pfaff_PNAS2013 _Saveframe_category entry_citation _Citation_full . _Citation_title 'Structural features of Argonaute-GW182 protein interactions.' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 24043833 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Pfaff Janina . . 2 Hennig Janosch . . 3 Herzog Franz . . 4 Aebersold Ruedi . . 5 Sattler Michael . . 6 Niessing Dierk . . 7 Meister Gunter . . stop_ _Journal_abbreviation 'Proc. Natl. Acad. Sci. U.S.A.' _Journal_name_full 'Proceedings of the National Academy of Sciences of the United States of America' _Journal_volume 110 _Journal_issue 40 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first . _Page_last . _Year 2013 _Details . loop_ _Keyword 'Argonaute proteins' 'gene regulation' 'gene silencing' 'GW182 proteins' microRNA stop_ save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name TNRC6Bm1 _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label TNRC6Bm1 $TNRC6Bm1 stop_ _System_molecular_weight . _System_physical_state 'intrinsically disordered' _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_TNRC6Bm1 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common TNRC6Bm1 _Molecular_mass . _Mol_thiol_state 'all free' loop_ _Biological_function 'TNRC6B is a gene regulator involved in gene silencing via Argonaute interactions' stop_ _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 90 _Mol_residue_sequence ; GPDSMDCQAVLQTLLSRTDL DPRVLSNTGWGQTQIKQDTV WDIEEVPRPEGKSDKGTEGW ESAATQTKNSGGWGDAPSQS NQMKSGWGEL ; loop_ _Residue_seq_code _Residue_label 1 GLY 2 PRO 3 ASP 4 SER 5 MET 6 ASP 7 CYS 8 GLN 9 ALA 10 VAL 11 LEU 12 GLN 13 THR 14 LEU 15 LEU 16 SER 17 ARG 18 THR 19 ASP 20 LEU 21 ASP 22 PRO 23 ARG 24 VAL 25 LEU 26 SER 27 ASN 28 THR 29 GLY 30 TRP 31 GLY 32 GLN 33 THR 34 GLN 35 ILE 36 LYS 37 GLN 38 ASP 39 THR 40 VAL 41 TRP 42 ASP 43 ILE 44 GLU 45 GLU 46 VAL 47 PRO 48 ARG 49 PRO 50 GLU 51 GLY 52 LYS 53 SER 54 ASP 55 LYS 56 GLY 57 THR 58 GLU 59 GLY 60 TRP 61 GLU 62 SER 63 ALA 64 ALA 65 THR 66 GLN 67 THR 68 LYS 69 ASN 70 SER 71 GLY 72 GLY 73 TRP 74 GLY 75 ASP 76 ALA 77 PRO 78 SER 79 GLN 80 SER 81 ASN 82 GLN 83 MET 84 LYS 85 SER 86 GLY 87 TRP 88 GLY 89 GLU 90 LEU stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $TNRC6Bm1 Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $TNRC6Bm1 'recombinant technology' . Escherichia coli 'Bl21(DE3) Gold pRARE' pET-TNRC6Bm1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details '5 C, pH 6.5' loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $TNRC6Bm1 . uM 100 300 '[U-13C; U-15N]' D2O 10 % . . '[U-99% 2H]' H2O 90 % . . 'natural abundance' 'sodium phosphate' 20 mM . . 'natural abundance' 'sodium chloride' 250 mM . . 'natural abundance' 'sodium azide' 0.02 % . . 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_CARA _Saveframe_category software _Name CARA _Version . loop_ _Vendor _Address _Electronic_address 'Keller and Wuthrich' . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model 'Avance III' _Field_strength 800 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_HNCA_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCA' _Sample_label $sample_1 save_ save_3D_HNCACB_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ save_3D_CBCA(CO)NH_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 6.5 . pH pressure 1 . atm temperature 278 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_TNRC6Bm1_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.00 na indirect . . . 0.251449530 DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 DSS N 15 'methyl protons' ppm 0.00 na indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_TNRC6Bm1_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' '3D HNCA' '3D HNCACB' '3D CBCA(CO)NH' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $TNRC6Bm1_reference_1 _Mol_system_component_name TNRC6Bm1 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 3 3 ASP H H 8.670 0.020 1 2 3 3 ASP CA C 53.957 0.3 1 3 3 3 ASP CB C 39.845 0.3 1 4 3 3 ASP N N 120.133 0.3 1 5 4 4 SER H H 8.268 0.020 1 6 4 4 SER CA C 57.667 0.3 1 7 4 4 SER CB C 62.412 0.3 1 8 4 4 SER N N 116.171 0.3 1 9 6 6 ASP H H 8.404 0.020 1 10 6 6 ASP CA C 53.940 0.3 1 11 6 6 ASP CB C 39.889 0.3 1 12 6 6 ASP N N 121.493 0.3 1 13 7 7 CYS H H 8.386 0.020 1 14 7 7 CYS CA C 57.728 0.3 1 15 7 7 CYS CB C 26.768 0.3 1 16 7 7 CYS N N 119.780 0.3 1 17 8 8 GLN H H 8.522 0.020 1 18 8 8 GLN CA C 54.924 0.3 1 19 8 8 GLN CB C 28.574 0.3 1 20 8 8 GLN N N 122.461 0.3 1 21 9 9 ALA H H 8.302 0.020 1 22 9 9 ALA CA C 52.015 0.3 1 23 9 9 ALA CB C 18.274 0.3 1 24 9 9 ALA N N 123.031 0.3 1 25 10 10 VAL H H 8.242 0.020 1 26 10 10 VAL CA C 61.422 0.3 1 27 10 10 VAL CB C 31.495 0.3 1 28 10 10 VAL N N 120.316 0.3 1 29 11 11 LEU H H 8.434 0.020 1 30 11 11 LEU CA C 53.940 0.3 1 31 11 11 LEU CB C 41.592 0.3 1 32 11 11 LEU N N 126.881 0.3 1 33 13 13 THR H H 7.972 0.020 1 34 13 13 THR CA C 62.334 0.3 1 35 13 13 THR CB C 69.876 0.3 1 36 13 13 THR N N 121.591 0.3 1 37 15 15 LEU H H 8.763 0.020 1 38 15 15 LEU CA C 54.001 0.3 1 39 15 15 LEU CB C 41.714 0.3 1 40 15 15 LEU N N 125.447 0.3 1 41 16 16 SER H H 8.644 0.020 1 42 16 16 SER CA C 56.755 0.3 1 43 16 16 SER CB C 63.324 0.3 1 44 16 16 SER N N 118.482 0.3 1 45 17 17 ARG H H 8.623 0.020 1 46 17 17 ARG CA C 55.826 0.3 1 47 17 17 ARG CB C 29.670 0.3 1 48 17 17 ARG N N 123.483 0.3 1 49 18 18 THR H H 8.387 0.020 1 50 18 18 THR CA C 61.439 0.3 1 51 18 18 THR CB C 68.799 0.3 1 52 18 18 THR N N 114.375 0.3 1 53 19 19 ASP H H 8.424 0.020 1 54 19 19 ASP CA C 53.940 0.3 1 55 19 19 ASP CB C 39.889 0.3 1 56 19 19 ASP N N 122.213 0.3 1 57 20 20 LEU H H 8.049 0.020 1 58 20 20 LEU CA C 53.940 0.3 1 59 20 20 LEU CB C 41.714 0.3 1 60 20 20 LEU N N 121.479 0.3 1 61 21 21 ASP H H 8.341 0.020 1 62 21 21 ASP CA C 51.142 0.3 1 63 21 21 ASP CB C 40.801 0.3 1 64 21 21 ASP N N 123.275 0.3 1 65 23 23 ARG H H 8.494 0.020 1 66 23 23 ARG CA C 55.903 0.3 1 67 23 23 ARG CB C 29.566 0.3 1 68 23 23 ARG N N 119.831 0.3 1 69 24 24 VAL H H 7.966 0.020 1 70 24 24 VAL CA C 61.864 0.3 1 71 24 24 VAL CB C 31.451 0.3 1 72 24 24 VAL N N 120.133 0.3 1 73 25 25 LEU H H 8.234 0.020 1 74 25 25 LEU CA C 54.244 0.3 1 75 25 25 LEU CB C 40.862 0.3 1 76 25 25 LEU N N 125.150 0.3 1 77 26 26 SER H H 8.248 0.020 1 78 26 26 SER CA C 57.667 0.3 1 79 26 26 SER CB C 62.838 0.3 1 80 26 26 SER N N 115.830 0.3 1 81 27 27 ASN H H 8.496 0.020 1 82 27 27 ASN CA C 52.480 0.3 1 83 27 27 ASN CB C 37.943 0.3 1 84 27 27 ASN N N 120.913 0.3 1 85 28 28 THR H H 8.171 0.020 1 86 28 28 THR CA C 61.439 0.3 1 87 28 28 THR CB C 68.920 0.3 1 88 28 28 THR N N 113.272 0.3 1 89 29 29 GLY H H 8.434 0.020 1 90 29 29 GLY CA C 44.590 0.3 1 91 29 29 GLY N N 110.964 0.3 1 92 32 32 GLN H H 8.193 0.020 1 93 32 32 GLN CA C 54.930 0.3 1 94 32 32 GLN CB C 28.592 0.3 1 95 32 32 GLN N N 119.713 0.3 1 96 33 33 THR H H 8.287 0.020 1 97 33 33 THR CA C 61.439 0.3 1 98 33 33 THR CB C 68.920 0.3 1 99 33 33 THR N N 115.500 0.3 1 100 34 34 GLN H H 8.464 0.020 1 101 34 34 GLN CA C 54.852 0.3 1 102 34 34 GLN CB C 28.636 0.3 1 103 34 34 GLN N N 123.165 0.3 1 104 35 35 ILE H H 8.304 0.020 1 105 35 35 ILE CA C 60.509 0.3 1 106 35 35 ILE CB C 37.943 0.3 1 107 35 35 ILE N N 123.136 0.3 1 108 36 36 LYS H H 8.538 0.020 1 109 36 36 LYS CA C 55.339 0.3 1 110 36 36 LYS CB C 32.286 0.3 1 111 36 36 LYS N N 126.658 0.3 1 112 37 37 GLN H H 8.619 0.020 1 113 37 37 GLN CA C 54.913 0.3 1 114 37 37 GLN CB C 28.636 0.3 1 115 37 37 GLN N N 123.056 0.3 1 116 38 38 ASP H H 8.568 0.020 1 117 38 38 ASP CA C 54.062 0.3 1 118 38 38 ASP CB C 39.950 0.3 1 119 38 38 ASP N N 122.114 0.3 1 120 39 39 THR H H 8.178 0.020 1 121 39 39 THR CA C 61.439 0.3 1 122 39 39 THR CB C 68.920 0.3 1 123 39 39 THR N N 115.098 0.3 1 124 40 40 VAL H H 8.197 0.020 1 125 40 40 VAL CA C 61.439 0.3 1 126 40 40 VAL CB C 31.451 0.3 1 127 40 40 VAL N N 122.987 0.3 1 128 41 41 TRP H H 8.235 0.020 1 129 41 41 TRP CA C 55.826 0.3 1 130 41 41 TRP CB C 28.697 0.3 1 131 41 41 TRP N N 125.199 0.3 1 132 42 42 ASP H H 8.297 0.020 1 133 42 42 ASP CA C 53.149 0.3 1 134 42 42 ASP CB C 39.889 0.3 1 135 42 42 ASP N N 122.610 0.3 1 136 43 43 ILE H H 8.056 0.020 1 137 43 43 ILE CA C 60.465 0.3 1 138 43 43 ILE CB C 37.960 0.3 1 139 43 43 ILE N N 120.989 0.3 1 140 44 44 GLU H H 8.503 0.020 1 141 44 44 GLU CA C 55.826 0.3 1 142 44 44 GLU CB C 29.549 0.3 1 143 44 44 GLU N N 124.693 0.3 1 144 45 45 GLU H H 8.452 0.020 1 145 45 45 GLU CA C 55.278 0.3 1 146 45 45 GLU CB C 29.549 0.3 1 147 45 45 GLU N N 122.987 0.3 1 148 46 46 VAL H H 8.322 0.020 1 149 46 46 VAL CA C 59.353 0.3 1 150 46 46 VAL CB C 31.495 0.3 1 151 46 46 VAL N N 123.939 0.3 1 152 48 48 ARG H H 8.570 0.020 1 153 48 48 ARG CA C 52.967 0.3 1 154 48 48 ARG CB C 29.366 0.3 1 155 48 48 ARG N N 123.066 0.3 1 156 50 50 GLU H H 8.758 0.020 1 157 50 50 GLU CA C 55.826 0.3 1 158 50 50 GLU CB C 29.549 0.3 1 159 50 50 GLU N N 121.509 0.3 1 160 51 51 GLY H H 8.515 0.020 1 161 51 51 GLY CA C 44.529 0.3 1 162 51 51 GLY N N 110.372 0.3 1 163 52 52 LYS H H 8.313 0.020 1 164 52 52 LYS CA C 54.913 0.3 1 165 52 52 LYS CB C 32.347 0.3 1 166 52 52 LYS N N 121.122 0.3 1 167 53 53 SER H H 8.546 0.020 1 168 53 53 SER CA C 57.667 0.3 1 169 53 53 SER CB C 63.263 0.3 1 170 53 53 SER N N 117.584 0.3 1 171 54 54 ASP H H 8.498 0.020 1 172 54 54 ASP CA C 53.149 0.3 1 173 54 54 ASP CB C 39.889 0.3 1 174 54 54 ASP N N 122.907 0.3 1 175 55 55 LYS H H 8.380 0.020 1 176 55 55 LYS CA C 55.826 0.3 1 177 55 55 LYS CB C 31.495 0.3 1 178 55 55 LYS N N 121.717 0.3 1 179 56 56 GLY H H 8.461 0.020 1 180 56 56 GLY CA C 44.529 0.3 1 181 56 56 GLY N N 109.985 0.3 1 182 57 57 THR H H 8.115 0.020 1 183 57 57 THR CA C 60.830 0.3 1 184 57 57 THR CB C 68.920 0.3 1 185 57 57 THR N N 113.313 0.3 1 186 58 58 GLU H H 8.668 0.020 1 187 58 58 GLU CA C 56.738 0.3 1 188 58 58 GLU CB C 28.697 0.3 1 189 58 58 GLU N N 123.423 0.3 1 190 59 59 GLY H H 8.641 0.020 1 191 59 59 GLY CA C 44.529 0.3 1 192 59 59 GLY N N 110.498 0.3 1 193 60 60 TRP H H 8.009 0.020 1 194 60 60 TRP CA C 56.755 0.3 1 195 60 60 TRP CB C 28.592 0.3 1 196 60 60 TRP N N 121.040 0.3 1 197 61 61 GLU H H 8.346 0.020 1 198 61 61 GLU CA C 55.886 0.3 1 199 61 61 GLU CB C 29.244 0.3 1 200 61 61 GLU N N 122.838 0.3 1 201 62 62 SER H H 8.183 0.020 1 202 62 62 SER CA C 57.667 0.3 1 203 62 62 SER CB C 62.777 0.3 1 204 62 62 SER N N 116.460 0.3 1 205 63 63 ALA H H 8.339 0.020 1 206 63 63 ALA CA C 52.054 0.3 1 207 63 63 ALA CB C 18.356 0.3 1 208 63 63 ALA N N 125.844 0.3 1 209 64 64 ALA H H 8.279 0.020 1 210 64 64 ALA CA C 52.054 0.3 1 211 64 64 ALA CB C 18.296 0.3 1 212 64 64 ALA N N 122.501 0.3 1 213 65 65 THR H H 8.087 0.020 1 214 65 65 THR CA C 61.439 0.3 1 215 65 65 THR CB C 68.859 0.3 1 216 65 65 THR N N 113.137 0.3 1 217 66 66 GLN H H 8.366 0.020 1 218 66 66 GLN CA C 54.852 0.3 1 219 66 66 GLN CB C 28.636 0.3 1 220 66 66 GLN N N 122.669 0.3 1 221 67 67 THR H H 8.327 0.020 1 222 67 67 THR CA C 61.439 0.3 1 223 67 67 THR CB C 68.920 0.3 1 224 67 67 THR N N 116.171 0.3 1 225 68 68 LYS H H 8.459 0.020 1 226 68 68 LYS CA C 55.826 0.3 1 227 68 68 LYS CB C 32.164 0.3 1 228 68 68 LYS N N 123.939 0.3 1 229 69 69 ASN H H 8.572 0.020 1 230 69 69 ASN CA C 52.132 0.3 1 231 69 69 ASN CB C 37.960 0.3 1 232 69 69 ASN N N 120.032 0.3 1 233 70 70 SER H H 8.427 0.020 1 234 70 70 SER CA C 57.728 0.3 1 235 70 70 SER CB C 62.655 0.3 1 236 70 70 SER N N 116.697 0.3 1 237 71 71 GLY H H 8.549 0.020 1 238 71 71 GLY CA C 44.529 0.3 1 239 71 71 GLY N N 111.332 0.3 1 240 72 72 GLY H H 8.256 0.020 1 241 72 72 GLY CA C 44.529 0.3 1 242 72 72 GLY N N 108.787 0.3 1 243 75 75 ASP H H 8.146 0.020 1 244 75 75 ASP CA C 52.984 0.3 1 245 75 75 ASP CB C 39.967 0.3 1 246 75 75 ASP N N 120.418 0.3 1 247 76 76 ALA H H 8.286 0.020 1 248 76 76 ALA CA C 50.108 0.3 1 249 76 76 ALA CB C 17.383 0.3 1 250 76 76 ALA N N 125.249 0.3 1 251 78 78 SER H H 8.501 0.020 1 252 78 78 SER CA C 57.728 0.3 1 253 78 78 SER CB C 63.324 0.3 1 254 78 78 SER N N 116.068 0.3 1 255 79 79 GLN H H 8.512 0.020 1 256 79 79 GLN CA C 54.913 0.3 1 257 79 79 GLN CB C 28.636 0.3 1 258 79 79 GLN N N 122.203 0.3 1 259 80 80 SER H H 8.392 0.020 1 260 80 80 SER CA C 57.667 0.3 1 261 80 80 SER CB C 62.473 0.3 1 262 80 80 SER N N 116.532 0.3 1 263 82 82 GLN H H 8.341 0.020 1 264 82 82 GLN CA C 55.052 0.3 1 265 82 82 GLN CB C 28.532 0.3 1 266 82 82 GLN N N 120.167 0.3 1 267 83 83 MET H H 8.151 0.020 1 268 83 83 MET CA C 56.677 0.3 1 269 83 83 MET CB C 32.407 0.3 1 270 83 83 MET N N 127.462 0.3 1 271 85 85 SER H H 8.437 0.020 1 272 85 85 SER CA C 57.667 0.3 1 273 85 85 SER CB C 62.473 0.3 1 274 85 85 SER N N 117.130 0.3 1 275 86 86 GLY H H 8.504 0.020 1 276 86 86 GLY CA C 44.529 0.3 1 277 86 86 GLY N N 110.768 0.3 1 278 89 89 GLU H H 8.075 0.020 1 279 89 89 GLU CA C 55.052 0.3 1 280 89 89 GLU CB C 29.566 0.3 1 281 89 89 GLU N N 120.452 0.3 1 282 90 90 LEU H H 8.020 0.020 1 283 90 90 LEU CA C 55.826 0.3 1 284 90 90 LEU CB C 42.565 0.3 1 285 90 90 LEU N N 128.795 0.3 1 stop_ save_