data_25078 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; CSD1-UNR bound to msl2 mRNA and Sex-lethal ; _BMRB_accession_number 25078 _BMRB_flat_file_name bmr25078.str _Entry_type original _Submission_date 2014-07-04 _Accession_date 2014-07-04 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details 'Cold shock domain 1 (CSD1) of protein Upstream-of-N-Ras (UNR) in complex with Sex-lethal (SXL) and msl2 mRNA' loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Hennig Janosch . . 2 Wang Iren . . 3 Sattler Michael . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 64 "13C chemical shifts" 91 "15N chemical shifts" 62 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2014-11-07 original author . stop_ loop_ _Related_BMRB_accession_number _Relationship 25059 'CSD1 in the free state' 25060 'CSD1 bound to RNA' 25072 SXL-UNR-msl2-mRNA stop_ _Original_release_date 2014-11-07 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Structural basis for the assembly of the Sxl-Unr translation regulatory complex' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 25209665 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Hennig Janosch . . 2 Militti Cristina . . 3 Popowicz Grzegorz M. . 4 Wang Iren . . 5 Sonntag Miriam . . 6 Geerlof Arie . . 7 Gabel Frank . . 8 Gebauer Fatima . . 9 Sattler Michael . . stop_ _Journal_abbreviation Nature _Journal_volume 515 _Journal_issue 7526 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 287 _Page_last 290 _Year 2014 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'CSD1-SXL-18-mer msl2 mRNA' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label CSD1 $UNR stop_ _System_molecular_weight 33500 _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details 'The assembly contains SXL-RRM1-RRM2 and 18-mer_RNA.' save_ ######################## # Monomeric polymers # ######################## save_UNR _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common UNR _Molecular_mass . _Mol_thiol_state 'all free' loop_ _Biological_function 'dosage compensation in Drosophila' stop_ _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 72 _Mol_residue_sequence ; GAMATRETGIIEKLLHSYGF IQCCERQARLFFHFSQFSGN IDHLKIGDPVEFEMTYDRRT GKPIASQVSKIA ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 181 GLY 2 182 ALA 3 183 MET 4 184 ALA 5 185 THR 6 186 ARG 7 187 GLU 8 188 THR 9 189 GLY 10 190 ILE 11 191 ILE 12 192 GLU 13 193 LYS 14 194 LEU 15 195 LEU 16 196 HIS 17 197 SER 18 198 TYR 19 199 GLY 20 200 PHE 21 201 ILE 22 202 GLN 23 203 CYS 24 204 CYS 25 205 GLU 26 206 ARG 27 207 GLN 28 208 ALA 29 209 ARG 30 210 LEU 31 211 PHE 32 212 PHE 33 213 HIS 34 214 PHE 35 215 SER 36 216 GLN 37 217 PHE 38 218 SER 39 219 GLY 40 220 ASN 41 221 ILE 42 222 ASP 43 223 HIS 44 224 LEU 45 225 LYS 46 226 ILE 47 227 GLY 48 228 ASP 49 229 PRO 50 230 VAL 51 231 GLU 52 232 PHE 53 233 GLU 54 234 MET 55 235 THR 56 236 TYR 57 237 ASP 58 238 ARG 59 239 ARG 60 240 THR 61 241 GLY 62 242 LYS 63 243 PRO 64 244 ILE 65 245 ALA 66 246 SER 67 247 GLN 68 248 VAL 69 249 SER 70 250 LYS 71 251 ILE 72 252 ALA stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-02-04 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 25059 dCSD1 100.00 72 100.00 100.00 1.32e-45 BMRB 25060 cold_shock_domain_1_of_upstream_of_N-ras 100.00 72 100.00 100.00 1.32e-45 PDB 4QQB "Structural Basis For The Assembly Of The Sxl-unr Translation Regulatory Complex" 100.00 72 100.00 100.00 1.32e-45 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $UNR 'fruit fly' 7227 Eukaryota Metazoa Drosophila melanogaster stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $UNR 'recombinant technology' . Escherichia coli Bl21 pTRX-CSD stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $UNR . mM 0.2 0.5 '[U-13C; U-15N; U-2H]' SXL-RRM1-RRM2 . mM 0.2 0.5 'natural abundance' '18-mer RNA' . mM 0.2 0.5 'natural abundance' DTT 10 mM . . 'natural abundance' 'potassium phosphate' 10 mM . . 'natural abundance' 'sodium chloride' 50 mM . . 'natural abundance' 'sodium azide' 0.02 % . . 'natural abundance' 'sodium chloride' 90 % . . 'natural abundance' 'sodium azide' 10 % . . 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_CARA _Saveframe_category software _Name CARA _Version . loop_ _Vendor _Address _Electronic_address 'Fred Damberger' . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 750 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_TROSY-HNCA_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D TROSY-HNCA' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units temperature 310 . K pH 6.0 . pH pressure 1 . atm stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 DSS C 13 'methyl protons' ppm 0.00 na indirect . . . 0.251449530 DSS N 15 'methyl protons' ppm 0.00 na indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' '3D TROSY-HNCA' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name CSD1 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 183 3 MET H H 8.379 0.020 1 2 183 3 MET C C 174.787 0.300 1 3 183 3 MET CA C 54.591 0.300 1 4 183 3 MET N N 120.278 0.300 1 5 184 4 ALA H H 8.649 0.020 1 6 184 4 ALA C C 177.149 0.300 1 7 184 4 ALA CA C 51.740 0.300 1 8 184 4 ALA N N 130.004 0.300 1 9 185 5 THR H H 8.137 0.020 1 10 185 5 THR CA C 61.672 0.300 1 11 185 5 THR N N 115.379 0.300 1 12 187 7 GLU H H 8.505 0.020 1 13 187 7 GLU C C 173.191 0.300 1 14 187 7 GLU CA C 53.933 0.300 1 15 187 7 GLU N N 121.132 0.300 1 16 188 8 THR H H 7.973 0.020 1 17 188 8 THR CA C 60.012 0.300 1 18 188 8 THR N N 106.326 0.300 1 19 189 9 GLY H H 9.048 0.020 1 20 189 9 GLY C C 170.891 0.300 1 21 189 9 GLY CA C 46.100 0.300 1 22 189 9 GLY N N 109.427 0.300 1 23 190 10 ILE H H 8.626 0.020 1 24 190 10 ILE C C 176.383 0.300 1 25 190 10 ILE CA C 58.006 0.300 1 26 190 10 ILE N N 118.776 0.300 1 27 191 11 ILE H H 8.503 0.020 1 28 191 11 ILE CA C 60.513 0.300 1 29 191 11 ILE N N 124.579 0.300 1 30 192 12 GLU H H 9.048 0.020 1 31 192 12 GLU CA C 56.534 0.300 1 32 192 12 GLU N N 131.953 0.300 1 33 193 13 LYS H H 7.460 0.020 1 34 193 13 LYS C C 173.339 0.300 1 35 193 13 LYS CA C 55.970 0.300 1 36 193 13 LYS N N 118.090 0.300 1 37 194 14 LEU H H 8.425 0.020 1 38 194 14 LEU CA C 54.560 0.300 1 39 194 14 LEU N N 125.299 0.300 1 40 198 18 TYR H H 7.964 0.020 1 41 198 18 TYR C C 170.953 0.300 1 42 198 18 TYR CA C 54.905 0.300 1 43 198 18 TYR N N 123.918 0.300 1 44 199 19 GLY H H 7.525 0.020 1 45 199 19 GLY C C 168.818 0.300 1 46 199 19 GLY CA C 45.348 0.300 1 47 199 19 GLY N N 104.632 0.300 1 48 200 20 PHE H H 8.539 0.020 1 49 200 20 PHE C C 175.326 0.300 1 50 200 20 PHE CA C 56.503 0.300 1 51 200 20 PHE N N 112.320 0.300 1 52 201 21 ILE H H 9.466 0.020 1 53 201 21 ILE C C 175.098 0.300 1 54 201 21 ILE CA C 59.228 0.300 1 55 201 21 ILE N N 124.313 0.300 1 56 202 22 GLN H H 9.122 0.020 1 57 202 22 GLN HE21 H 6.709 0.020 1 58 202 22 GLN HE22 H 7.954 0.020 1 59 202 22 GLN C C 175.740 0.300 1 60 202 22 GLN CA C 54.247 0.300 1 61 202 22 GLN N N 126.911 0.300 1 62 202 22 GLN NE2 N 111.819 0.300 1 63 203 23 CYS H H 8.507 0.020 1 64 203 23 CYS CA C 62.424 0.300 1 65 203 23 CYS N N 127.704 0.300 1 66 204 24 CYS H H 8.737 0.020 1 67 204 24 CYS N N 118.727 0.300 1 68 205 25 GLU H H 9.104 0.020 1 69 205 25 GLU C C 174.704 0.300 1 70 205 25 GLU CA C 56.503 0.300 1 71 205 25 GLU N N 120.074 0.300 1 72 206 26 ARG H H 7.476 0.020 1 73 206 26 ARG CA C 53.589 0.300 1 74 206 26 ARG N N 114.709 0.300 1 75 208 28 ALA H H 7.870 0.020 1 76 208 28 ALA C C 173.651 0.300 1 77 208 28 ALA CA C 51.991 0.300 1 78 208 28 ALA N N 124.927 0.300 1 79 209 29 ARG H H 8.250 0.020 1 80 209 29 ARG C C 175.699 0.300 1 81 209 29 ARG CA C 54.278 0.300 1 82 209 29 ARG N N 123.232 0.300 1 83 210 30 LEU H H 8.909 0.020 1 84 210 30 LEU C C 175.574 0.300 1 85 210 30 LEU CA C 52.774 0.300 1 86 210 30 LEU N N 125.051 0.300 1 87 211 31 PHE H H 8.531 0.020 1 88 211 31 PHE C C 173.523 0.300 1 89 211 31 PHE CA C 56.691 0.300 1 90 211 31 PHE N N 125.271 0.300 1 91 212 32 PHE H H 7.559 0.020 1 92 212 32 PHE C C 170.269 0.300 1 93 212 32 PHE CA C 54.999 0.300 1 94 212 32 PHE N N 123.191 0.300 1 95 213 33 HIS H H 8.844 0.020 1 96 213 33 HIS C C 177.439 0.300 1 97 213 33 HIS CA C 55.970 0.300 1 98 213 33 HIS N N 121.207 0.300 1 99 214 34 PHE H H 7.652 0.020 1 100 214 34 PHE CA C 57.568 0.300 1 101 214 34 PHE N N 125.797 0.300 1 102 216 36 GLN H H 8.169 0.020 1 103 216 36 GLN C C 174.310 0.300 1 104 216 36 GLN CA C 54.717 0.300 1 105 216 36 GLN N N 120.030 0.300 1 106 217 37 PHE H H 7.876 0.020 1 107 217 37 PHE C C 174.020 0.300 1 108 217 37 PHE CA C 56.628 0.300 1 109 217 37 PHE N N 122.215 0.300 1 110 218 38 SER H H 8.069 0.020 1 111 218 38 SER C C 172.466 0.300 1 112 218 38 SER CA C 56.659 0.300 1 113 218 38 SER N N 124.133 0.300 1 114 219 39 GLY H H 7.117 0.020 1 115 219 39 GLY CA C 43.656 0.300 1 116 219 39 GLY N N 110.915 0.300 1 117 221 41 ILE H H 8.649 0.020 1 118 221 41 ILE C C 176.217 0.300 1 119 221 41 ILE CA C 61.578 0.300 1 120 221 41 ILE N N 128.288 0.300 1 121 222 42 ASP H H 7.980 0.020 1 122 222 42 ASP C C 176.818 0.300 1 123 222 42 ASP CA C 55.531 0.300 1 124 222 42 ASP N N 119.911 0.300 1 125 223 43 HIS H H 7.424 0.020 1 126 223 43 HIS C C 173.543 0.300 1 127 223 43 HIS CA C 53.714 0.300 1 128 223 43 HIS N N 115.346 0.300 1 129 224 44 LEU H H 7.111 0.020 1 130 224 44 LEU C C 174.393 0.300 1 131 224 44 LEU CA C 54.497 0.300 1 132 224 44 LEU N N 123.728 0.300 1 133 225 45 LYS H H 8.639 0.020 1 134 225 45 LYS C C 175.719 0.300 1 135 225 45 LYS CA C 53.589 0.300 1 136 225 45 LYS N N 125.299 0.300 1 137 226 46 ILE H H 7.954 0.020 1 138 226 46 ILE C C 177.315 0.300 1 139 226 46 ILE CA C 62.456 0.300 1 140 226 46 ILE N N 118.622 0.300 1 141 227 47 GLY H H 9.003 0.020 1 142 227 47 GLY C C 173.999 0.300 1 143 227 47 GLY CA C 44.220 0.300 1 144 227 47 GLY N N 115.585 0.300 1 145 228 48 ASP H H 7.842 0.020 1 146 228 48 ASP CA C 53.025 0.300 1 147 228 48 ASP N N 122.050 0.300 1 148 230 50 VAL H H 8.721 0.020 1 149 230 50 VAL CA C 58.069 0.300 1 150 230 50 VAL N N 114.676 0.300 1 151 231 51 GLU H H 8.976 0.020 1 152 231 51 GLU C C 174.435 0.300 1 153 231 51 GLU CA C 53.244 0.300 1 154 231 51 GLU N N 120.320 0.300 1 155 232 52 PHE H H 8.103 0.020 1 156 232 52 PHE CA C 56.283 0.300 1 157 232 52 PHE N N 117.569 0.300 1 158 233 53 GLU H H 8.641 0.020 1 159 233 53 GLU CA C 53.620 0.300 1 160 233 53 GLU N N 117.236 0.300 1 161 234 54 MET H H 9.547 0.020 1 162 234 54 MET C C 175.284 0.300 1 163 234 54 MET CA C 55.625 0.300 1 164 234 54 MET N N 129.721 0.300 1 165 235 55 THR H H 8.687 0.020 1 166 235 55 THR CA C 59.228 0.300 1 167 235 55 THR N N 121.815 0.300 1 168 236 56 TYR H H 8.369 0.020 1 169 236 56 TYR N N 117.693 0.300 1 170 237 57 ASP H H 8.734 0.020 1 171 237 57 ASP CA C 56.816 0.300 1 172 237 57 ASP N N 126.379 0.300 1 173 238 58 ARG H H 8.856 0.020 1 174 238 58 ARG N N 128.014 0.300 1 175 239 59 ARG H H 8.414 0.020 1 176 239 59 ARG CA C 57.317 0.300 1 177 239 59 ARG N N 115.709 0.300 1 178 241 61 GLY H H 8.426 0.020 1 179 241 61 GLY CA C 45.317 0.300 1 180 241 61 GLY N N 111.824 0.300 1 181 242 62 LYS H H 7.562 0.020 1 182 242 62 LYS CA C 54.309 0.300 1 183 242 62 LYS N N 119.843 0.300 1 184 244 64 ILE H H 8.969 0.020 1 185 244 64 ILE C C 173.958 0.300 1 186 244 64 ILE CA C 60.262 0.300 1 187 244 64 ILE N N 118.148 0.300 1 188 245 65 ALA H H 8.558 0.020 1 189 245 65 ALA CA C 50.267 0.300 1 190 245 65 ALA N N 121.372 0.300 1 191 246 66 SER H H 8.655 0.020 1 192 246 66 SER C C 172.777 0.300 1 193 246 66 SER CA C 55.751 0.300 1 194 246 66 SER N N 114.641 0.300 1 195 247 67 GLN H H 9.278 0.020 1 196 247 67 GLN HE21 H 6.802 0.020 1 197 247 67 GLN HE22 H 7.664 0.020 1 198 247 67 GLN C C 174.973 0.300 1 199 247 67 GLN CA C 56.064 0.300 1 200 247 67 GLN N N 120.173 0.300 1 201 247 67 GLN NE2 N 113.369 0.300 1 202 248 68 VAL H H 8.301 0.020 1 203 248 68 VAL CA C 61.296 0.300 1 204 248 68 VAL N N 121.083 0.300 1 205 249 69 SER H H 9.043 0.020 1 206 249 69 SER N N 118.561 0.300 1 207 250 70 LYS H H 8.748 0.020 1 208 250 70 LYS C C 176.839 0.300 1 209 250 70 LYS CA C 56.753 0.300 1 210 250 70 LYS N N 122.736 0.300 1 211 251 71 ILE H H 7.791 0.020 1 212 251 71 ILE C C 174.372 0.300 1 213 251 71 ILE CA C 61.547 0.300 1 214 251 71 ILE N N 124.803 0.300 1 215 252 72 ALA H H 7.740 0.020 1 216 252 72 ALA CA C 53.244 0.300 1 217 252 72 ALA N N 133.276 0.300 1 stop_ save_