data_25997 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Solution structure of the J domain of EMCV IRES ; _BMRB_accession_number 25997 _BMRB_flat_file_name bmr25997.str _Entry_type original _Submission_date 2016-03-16 _Accession_date 2016-06-10 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Imai Shunsuke . . 2 D'Souza Victoria . . 3 Wagner Gerhard . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 154 "13C chemical shifts" 143 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2016-10-12 update BMRB 'update entry citation' 2016-08-08 original author 'original release' stop_ loop_ _Related_BMRB_accession_number _Relationship 25999 'Solution structure of the St domain of EMCV IRES' 26000 'Solution structure of the delta-J-delta-K domain of EMCV IRES' 26996 'Solution structure of the J-K region of EMCV IRES' 26998 'Solution structure of the K domain of EMCV IRES' stop_ _Original_release_date 2016-08-08 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; An accurately preorganized IRES RNA structure enables eIF4G capture for initiation of viral translation ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 27525590 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Imai Shunsuke . . 2 Hellen Christopher . . 3 D'Souza Victoria . . 4 Wagner Gerhard . . stop_ _Journal_abbreviation 'Nat. Struct. Mol. Biol.' _Journal_volume 23 _Journal_issue 9 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 859 _Page_last 864 _Year 2016 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'J domain of EMCV IRES' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'RNA (39-MER)' $RNA_(39-MER) stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_RNA_(39-MER) _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class RNA _Name_common RNA_(39-MER) _Molecular_mass 12538.552 _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 39 _Mol_residue_sequence ; GGGCAGAAGGUACCCCAUUG UAUGGGAUCUGAUCUGCCC ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 693 G 2 694 G 3 695 G 4 696 C 5 697 A 6 698 G 7 699 A 8 700 A 9 701 G 10 702 G 11 703 U 12 704 A 13 705 C 14 706 C 15 707 C 16 708 C 17 709 A 18 710 U 19 711 U 20 712 G 21 713 U 22 714 A 23 715 U 24 716 G 25 717 G 26 718 G 27 719 A 28 720 U 29 721 C 30 722 U 31 723 G 32 724 A 33 725 U 34 726 C 35 727 U 36 728 G 37 729 C 38 730 C 39 731 C stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $RNA_(39-MER) 'Encephalomyocarditis virus' 12104 Viruses . Cardiovirus 'Cardiovirus A' stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $RNA_(39-MER) 'enzymatic semisynthesis' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $RNA_(39-MER) 0.5 mM 'natural abundance' 'potassium phosphate' 10 mM 'natural abundance' 'sodium chloride' 10 mM 'natural abundance' D2O 100 % [U-2H] stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $RNA_(39-MER) 0.5 mM 'natural abundance' 'potassium phosphate' 10 mM 'natural abundance' 'sodium chloride' 10 mM 'natural abundance' H2O 90 % 'natural abundance' D2O 10 % [U-2H] stop_ save_ save_sample_3 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $RNA_(39-MER) 0.5 mM '[U-13C; U-15N]-Ade, [U-13C; U-15N]-Cyt' 'potassium phosphate' 10 mM 'natural abundance' 'sodium chloride' 10 mM 'natural abundance' D2O 100 % [U-2H] stop_ save_ save_sample_4 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $RNA_(39-MER) 0.5 mM '[U-13C; U-15N]-Gua, [U-13C; U-15N]-Ura' 'potassium phosphate' 10 mM 'natural abundance' 'sodium chloride' 10 mM 'natural abundance' D2O 100 % [U-2H] stop_ save_ save_sample_5 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $RNA_(39-MER) 0.5 mM '[U-13C; U-15N]-Ade, [U-13C; U-15N]-Cyt' 'potassium phosphate' 10 mM 'natural abundance' 'sodium chloride' 10 mM 'natural abundance' H2O 90 % 'natural abundance' D2O 10 % [U-2H] stop_ save_ save_sample_6 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $RNA_(39-MER) 0.5 mM '[U-13C; U-15N]-Gua, [U-13C; U-15N]-Ura' 'potassium phosphate' 10 mM 'natural abundance' 'sodium chloride' 10 mM 'natural abundance' H2O 90 % 'natural abundance' D2O 10 % [U-2H] stop_ save_ ############################ # Computer software used # ############################ save_CARA _Saveframe_category software _Name CARA _Version . loop_ _Vendor _Address _Electronic_address 'Keller and Wuthrich' . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ save_CYANA _Saveframe_category software _Name CYANA _Version . loop_ _Vendor _Address _Electronic_address 'Guntert, Mumenthaler and Wuthrich' . . stop_ loop_ _Task 'structure solution' stop_ _Details . save_ save_X-PLOR_NIH _Saveframe_category software _Name X-PLOR_NIH _Version . loop_ _Vendor _Address _Electronic_address 'Schwieters, Kuszewski, Tjandra and Clore' . . stop_ loop_ _Task refinement stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 800 _Details . save_ save_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 700 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-1H_NOESY_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_1 save_ save_2D_1H-1H_NOESY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_2 save_ save_2D_1H-1H_NOESY_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_2 save_ save_2D_1H-13C_HSQC_4 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HSQC' _Sample_label $sample_3 save_ save_3D_1H-13C_NOESY_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-13C NOESY' _Sample_label $sample_3 save_ save_2D_1H-13C_HSQC_6 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HSQC' _Sample_label $sample_4 save_ save_3D_1H-13C_NOESY_7 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-13C NOESY' _Sample_label $sample_4 save_ save_2D_1H-15N_HSQC_8 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_5 save_ save_2D_1H-15N_HSQC_9 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_5 save_ save_2D_1H-15N_HSQC_10 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_6 save_ save_2D_1H-15N_HSQC_11 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_6 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 20 . mM pH 6.5 . pH pressure 1 . atm temperature 308 . K stop_ save_ save_sample_conditions_2 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 20 . mM pH 6.5 . pH pressure 1 . atm temperature 283 . K stop_ save_ save_sample_conditions_3 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 20 . mM pH 5.5 . pH pressure 1 . atm temperature 283 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.00 na indirect . . . 0.251449530 DSS H 1 'methyl protons' ppm 0 external direct . . . 1 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-1H NOESY' '2D 1H-13C HSQC' stop_ loop_ _Sample_label $sample_1 $sample_3 $sample_4 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'RNA (39-MER)' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 693 1 G H1' H 5.88 0 1 2 693 1 G H2' H 4.96 0 1 3 693 1 G H8 H 8.17 0 1 4 693 1 G C1' C 91.33 0 1 5 693 1 G C2' C 75.23 0 1 6 693 1 G C8 C 139.07 0 1 7 694 2 G H1' H 5.97 0 1 8 694 2 G H2' H 4.75 0 1 9 694 2 G H8 H 7.60 0 1 10 694 2 G C1' C 92.83 0 1 11 694 2 G C2' C 75.60 0 1 12 694 2 G C8 C 136.89 0 1 13 695 3 G H1' H 5.81 0 1 14 695 3 G H2' H 4.55 0 1 15 695 3 G H8 H 7.29 0 1 16 695 3 G C1' C 93.10 0 1 17 695 3 G C2' C 75.39 0 1 18 695 3 G C8 C 135.99 0 1 19 696 4 C H1' H 5.52 0 1 20 696 4 C H2' H 4.60 0 1 21 696 4 C H5 H 5.23 0 1 22 696 4 C H6 H 7.59 0 1 23 696 4 C C1' C 93.82 0 1 24 696 4 C C2' C 75.41 0 1 25 696 4 C C5 C 97.42 0 1 26 696 4 C C6 C 140.65 0 1 27 697 5 A H1' H 5.95 0 1 28 697 5 A H2 H 6.98 0 1 29 697 5 A H2' H 4.67 0 1 30 697 5 A H8 H 7.94 0 1 31 697 5 A C1' C 93.10 0 1 32 697 5 A C2 C 152.32 0 1 33 697 5 A C2' C 75.79 0 1 34 697 5 A C8 C 139.35 0 1 35 698 6 G H1' H 5.54 0 1 36 698 6 G H2' H 4.46 0 1 37 698 6 G H8 H 7.13 0 1 38 698 6 G C1' C 92.55 0 1 39 698 6 G C2' C 75.55 0 1 40 698 6 G C8 C 135.86 0 1 41 699 7 A H1' H 5.88 0 1 42 699 7 A H2 H 7.57 0 1 43 699 7 A H2' H 4.55 0 1 44 699 7 A H8 H 7.70 0 1 45 699 7 A C1' C 92.18 0 1 46 699 7 A C2 C 154.03 0 1 47 699 7 A C2' C 75.91 0 1 48 699 7 A C8 C 139.42 0 1 49 700 8 A H1' H 5.67 0 1 50 700 8 A H2 H 7.84 0 1 51 700 8 A H2' H 4.50 0 1 52 700 8 A H8 H 7.75 0 1 53 700 8 A C1' C 92.05 0 1 54 700 8 A C2 C 154.76 0 1 55 700 8 A C2' C 74.15 0 1 56 700 8 A C8 C 139.85 0 1 57 701 9 G H1' H 5.14 0 1 58 701 9 G H2' H 4.46 0 1 59 701 9 G H3' H 4.27 0 1 60 701 9 G H8 H 7.49 0 1 61 701 9 G C1' C 90.94 0 1 62 701 9 G C2' C 75.31 0 1 63 701 9 G C8 C 137.64 0 1 64 702 10 G H1' H 5.75 0 1 65 702 10 G H2' H 4.65 0 1 66 702 10 G H8 H 7.61 0 1 67 702 10 G C1' C 90.36 0 1 68 702 10 G C2' C 75.67 0 1 69 703 11 U H1' H 5.89 0 1 70 703 11 U H2' H 4.49 0 1 71 703 11 U H5 H 5.79 0 1 72 703 11 U H6 H 7.80 0 1 73 703 11 U C1' C 92.46 0 1 74 703 11 U C2' C 75.93 0 1 75 703 11 U C5 C 105.21 0 1 76 703 11 U C6 C 143.18 0 1 77 704 12 A H1' H 5.93 0 1 78 704 12 A H2 H 7.89 0 1 79 704 12 A H2' H 4.88 0 1 80 704 12 A H5' H 4.25 0 2 81 704 12 A H5'' H 4.48 0 2 82 704 12 A H8 H 8.16 0 1 83 704 12 A C1' C 93.13 0 1 84 704 12 A C2 C 154.16 0 1 85 704 12 A C2' C 75.46 0 1 86 704 12 A C5' C 66.65 0 1 87 704 12 A C8 C 141.39 0 1 88 705 13 C H1' H 5.51 0 1 89 705 13 C H2' H 4.25 0 1 90 705 13 C H5 H 5.60 0 1 91 705 13 C H6 H 7.77 0 1 92 705 13 C C1' C 93.34 0 1 93 705 13 C C2' C 75.78 0 1 94 705 13 C C5 C 97.67 0 1 95 705 13 C C6 C 141.62 0 1 96 706 14 C H1' H 5.65 0 1 97 706 14 C H2' H 4.39 0 1 98 706 14 C H5 H 5.69 0 1 99 706 14 C H6 H 7.81 0 1 100 706 14 C C1' C 93.69 0 1 101 706 14 C C2' C 75.63 0 1 102 706 14 C C5 C 98.40 0 1 103 706 14 C C6 C 141.93 0 1 104 707 15 C H1' H 5.46 0 1 105 707 15 C H2' H 4.04 0 1 106 707 15 C H5 H 5.53 0 1 107 707 15 C H6 H 7.83 0 1 108 707 15 C C1' C 94.32 0 1 109 707 15 C C2' C 67.58 0 1 110 707 15 C C5 C 98.11 0 1 111 707 15 C C6 C 141.72 0 1 112 708 16 C H1' H 5.45 0 1 113 708 16 C H2' H 4.46 0 1 114 708 16 C H5 H 5.52 0 1 115 708 16 C H6 H 7.77 0 1 116 708 16 C C1' C 94.15 0 1 117 708 16 C C2' C 75.24 0 1 118 708 16 C C5 C 97.49 0 1 119 708 16 C C6 C 141.37 0 1 120 709 17 A H1' H 5.96 0 1 121 709 17 A H2 H 7.48 0 1 122 709 17 A H2' H 4.39 0 1 123 709 17 A H8 H 8.06 0 1 124 709 17 A C1' C 93.15 0 1 125 709 17 A C2 C 153.84 0 1 126 709 17 A C2' C 75.86 0 1 127 709 17 A C8 C 139.64 0 1 128 710 18 U H1' H 5.67 0 1 129 710 18 U H2' H 4.26 0 1 130 710 18 U H5 H 5.21 0 1 131 710 18 U H6 H 7.39 0 1 132 710 18 U C1' C 90.47 0 1 133 710 18 U C2' C 75.75 0 1 134 710 18 U C5 C 104.45 0 1 135 710 18 U C6 C 141.63 0 1 136 711 19 U H1' H 5.87 0 1 137 711 19 U H2' H 4.36 0 1 138 711 19 U H5 H 5.86 0 1 139 711 19 U H6 H 7.86 0 1 140 711 19 U C1' C 90.89 0 1 141 711 19 U C2' C 76.19 0 1 142 711 19 U C5 C 105.05 0 1 143 711 19 U C6 C 143.53 0 1 144 712 20 G H1' H 5.40 0 1 145 712 20 G H2' H 4.42 0 1 146 712 20 G H3' H 4.59 0 1 147 712 20 G H4' H 3.87 0 1 148 712 20 G H5' H 3.99 0 2 149 712 20 G H5'' H 4.13 0 2 150 712 20 G H8 H 7.84 0 1 151 712 20 G C1' C 89.20 0 1 152 712 20 G C2' C 77.00 0 1 153 712 20 G C4' C 86.08 0 1 154 712 20 G C5' C 68.06 0 1 155 712 20 G C8 C 140.06 0 1 156 713 21 U H1' H 5.88 0 1 157 713 21 U H2' H 4.33 0 1 158 713 21 U H3' H 4.70 0 1 159 713 21 U H4' H 4.43 0 1 160 713 21 U H5 H 5.86 0 1 161 713 21 U H5' H 3.98 0 2 162 713 21 U H5'' H 3.92 0 2 163 713 21 U H6 H 7.74 0 1 164 713 21 U C1' C 90.05 0 1 165 713 21 U C2' C 75.70 0 1 166 713 21 U C3' C 77.58 0 1 167 713 21 U C5 C 105.52 0 1 168 713 21 U C5' C 68.08 0 1 169 713 21 U C6 C 143.94 0 1 170 714 22 A H1' H 5.92 0 1 171 714 22 A H2 H 7.94 0 1 172 714 22 A H2' H 4.87 0 1 173 714 22 A H3' H 4.76 0 1 174 714 22 A H4' H 4.61 0 1 175 714 22 A H5' H 4.27 0 2 176 714 22 A H5'' H 4.30 0 2 177 714 22 A H8 H 8.45 0 1 178 714 22 A C1' C 89.65 0 1 179 714 22 A C2 C 154.83 0 1 180 714 22 A C2' C 75.08 0 1 181 714 22 A C3' C 76.43 0 1 182 714 22 A C4' C 84.65 0 1 183 714 22 A C5' C 68.32 0 1 184 714 22 A C8 C 141.39 0 1 185 715 23 U H1' H 5.85 0 1 186 715 23 U H2' H 4.68 0 1 187 715 23 U H5 H 5.20 0 1 188 715 23 U H6 H 7.74 0 1 189 715 23 U C1' C 93.26 0 1 190 715 23 U C2' C 75.20 0 1 191 715 23 U C5 C 103.62 0 1 192 715 23 U C6 C 142.52 0 1 193 716 24 G H1' H 5.93 0 1 194 716 24 G H2' H 4.66 0 1 195 716 24 G H8 H 7.76 0 1 196 716 24 G C1' C 92.47 0 1 197 716 24 G C2' C 75.60 0 1 198 716 24 G C8 C 136.48 0 1 199 717 25 G H1' H 5.72 0 1 200 717 25 G H2' H 4.47 0 1 201 717 25 G H8 H 7.20 0 1 202 717 25 G C1' C 92.71 0 1 203 717 25 G C2' C 72.99 0 1 204 717 25 G C8 C 135.89 0 1 205 718 26 G H1' H 5.74 0 1 206 718 26 G H2' H 4.61 0 1 207 718 26 G H8 H 7.11 0 1 208 718 26 G C1' C 92.98 0 1 209 718 26 G C2' C 75.55 0 1 210 718 26 G C8 C 136.27 0 1 211 719 27 A H1' H 6.05 0 1 212 719 27 A H2 H 8.16 0 1 213 719 27 A H2' H 4.62 0 1 214 719 27 A H8 H 7.85 0 1 215 719 27 A C1' C 93.27 0 1 216 719 27 A C2' C 75.80 0 1 217 719 27 A C8 C 140.76 0 1 218 720 28 U H1' H 5.40 0 1 219 720 28 U H2' H 4.32 0 1 220 720 28 U H5 H 5.33 0 1 221 720 28 U H6 H 7.58 0 1 222 720 28 U C1' C 93.40 0 1 223 720 28 U C2' C 75.48 0 1 224 720 28 U C5 C 103.43 0 1 225 720 28 U C6 C 141.20 0 1 226 721 29 C H1' H 5.59 0 1 227 721 29 C H2' H 4.42 0 1 228 721 29 C H5 H 5.63 0 1 229 721 29 C H6 H 7.79 0 1 230 721 29 C C1' C 93.40 0 1 231 721 29 C C2' C 75.61 0 1 232 721 29 C C5 C 97.97 0 1 233 721 29 C C6 C 141.82 0 1 234 722 30 U H1' H 5.52 0 1 235 722 30 U H2' H 4.14 0 1 236 722 30 U H5 H 5.57 0 1 237 722 30 U H6 H 7.65 0 1 238 722 30 U C1' C 93.51 0 1 239 722 30 U C2' C 75.51 0 1 240 722 30 U C5 C 104.70 0 1 241 722 30 U C6 C 141.47 0 1 242 723 31 G H1' H 5.56 0 1 243 723 31 G H2' H 4.54 0 1 244 723 31 G H3' H 4.79 0 1 245 723 31 G H8 H 7.83 0 1 246 723 31 G C1' C 90.02 0 1 247 723 31 G C2' C 75.97 0 1 248 723 31 G C3' C 75.64 0 1 249 723 31 G C8 C 138.67 0 1 250 724 32 A H1' H 5.97 0 1 251 724 32 A H2 H 7.87 0 1 252 724 32 A H2' H 4.75 0 1 253 724 32 A H8 H 8.29 0 1 254 724 32 A C1' C 90.11 0 1 255 724 32 A C2 C 154.91 0 1 256 724 32 A C2' C 76.16 0 1 257 724 32 A C8 C 141.55 0 1 258 725 33 U H1' H 5.61 0 1 259 725 33 U H2' H 4.59 0 1 260 725 33 U H5 H 5.80 0 1 261 725 33 U H6 H 7.91 0 1 262 725 33 U C1' C 92.21 0 1 263 725 33 U C2' C 75.13 0 1 264 725 33 U C5 C 104.55 0 1 265 725 33 U C6 C 143.11 0 1 266 726 34 C H1' H 5.53 0 1 267 726 34 C H2' H 4.42 0 1 268 726 34 C H5 H 5.78 0 1 269 726 34 C H6 H 7.85 0 1 270 726 34 C C1' C 94.42 0 1 271 726 34 C C2' C 75.36 0 1 272 726 34 C C5 C 97.92 0 1 273 726 34 C C6 C 142.12 0 1 274 727 35 U H1' H 5.50 0 1 275 727 35 U H2' H 4.60 0 1 276 727 35 U H5 H 5.44 0 1 277 727 35 U H6 H 7.90 0 1 278 727 35 U C1' C 93.51 0 1 279 727 35 U C2' C 75.27 0 1 280 727 35 U C5 C 103.63 0 1 281 727 35 U C6 C 141.84 0 1 282 728 36 G H1' H 5.82 0 1 283 728 36 G H2' H 4.73 0 1 284 728 36 G H8 H 7.81 0 1 285 728 36 G C1' C 92.58 0 1 286 728 36 G C8 C 136.42 0 1 287 729 37 C H1' H 5.49 0 1 288 729 37 C H2' H 4.36 0 1 289 729 37 C H5 H 5.25 0 1 290 729 37 C H6 H 7.71 0 1 291 729 37 C C1' C 93.82 0 1 292 729 37 C C5 C 96.97 0 1 293 729 37 C C6 C 141.11 0 1 294 730 38 C H5 H 5.50 0 1 295 730 38 C H6 H 7.79 0 1 296 730 38 C C5 C 97.70 0 1 297 730 38 C C6 C 141.40 0 1 stop_ save_