data_26591 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; NMR Characterization of the Type III Secretion System Tip Chaperone Protein PcrG of Pseudomonas aeruginosa ; _BMRB_accession_number 26591 _BMRB_flat_file_name bmr26591.str _Entry_type original _Submission_date 2015-06-22 _Accession_date 2015-06-22 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Kaur Kawaljit . . 2 'De Guzman' Roberto . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 74 "13C chemical shifts" 145 "15N chemical shifts" 75 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2015-12-18 original BMRB . stop_ loop_ _Related_BMRB_accession_number _Relationship 26592 LcrG stop_ _Original_release_date 2015-12-18 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; The LcrG Tip Chaperone Protein of the Yersinia pestis Type III Secretion System Is Partially Folded ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 26259880 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Chaudhury Sukanya . . 2 Nordhues Bryce . . 3 Kaur Kawaljit . . 4 Zhang Na . . 5 'De Guzman' Roberto . . stop_ _Journal_abbreviation 'J. Mol. Biol.' _Journal_volume 427 _Journal_issue 19 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 3096 _Page_last 3109 _Year 2015 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name PcrG9-76 _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label PcrG9-76 $PcrG9-76 stop_ _System_molecular_weight 8520.5 _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_PcrG9-76 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common PcrG9-76 _Molecular_mass . _Mol_thiol_state 'not present' loop_ _Biological_function 'Chaperone to type III secretion system tip protein PcrV' stop_ _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 76 _Mol_residue_sequence ; EDTLRATVQAAELAIRDSEE RGRLLAEMWQGLGLAADAGE LLFQAPERELARAAEEELLA ELRRMRSSGSENLYFQ ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 9 GLU 2 10 ASP 3 11 THR 4 12 LEU 5 13 ARG 6 14 ALA 7 15 THR 8 16 VAL 9 17 GLN 10 18 ALA 11 19 ALA 12 20 GLU 13 21 LEU 14 22 ALA 15 23 ILE 16 24 ARG 17 25 ASP 18 26 SER 19 27 GLU 20 28 GLU 21 29 ARG 22 30 GLY 23 31 ARG 24 32 LEU 25 33 LEU 26 34 ALA 27 35 GLU 28 36 MET 29 37 TRP 30 38 GLN 31 39 GLY 32 40 LEU 33 41 GLY 34 42 LEU 35 43 ALA 36 44 ALA 37 45 ASP 38 46 ALA 39 47 GLY 40 48 GLU 41 49 LEU 42 50 LEU 43 51 PHE 44 52 GLN 45 53 ALA 46 54 PRO 47 55 GLU 48 56 ARG 49 57 GLU 50 58 LEU 51 59 ALA 52 60 ARG 53 61 ALA 54 62 ALA 55 63 GLU 56 64 GLU 57 65 GLU 58 66 LEU 59 67 LEU 60 68 ALA 61 69 GLU 62 70 LEU 63 71 ARG 64 72 ARG 65 73 MET 66 74 ARG 67 75 SER 68 76 SER 69 77 GLY 70 78 SER 71 79 GLU 72 80 ASN 73 81 LEU 74 82 TYR 75 83 PHE 76 84 GLN stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species _Strain $PcrG9-76 g-proteobacteria 208964 Bacteria . Pseudomonas aeruginosa PAO1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name _Details $PcrG9-76 'recombinant technology' . Escherichia coli . pET21a 'A modified pET21a with a C-terminal TEV cleavage site, followed by a GB1 and Hexa-His affinity tag was used' stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $PcrG9-76 0.7 mM '[U-99% 13C; U-99% 15N]' 'sodium chloride' . mM 'natural abundance' 'sodium phosphate' . mM 'natural abundance' D2O 10 mM 'natural abundance' H2O 90 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_NMRView _Saveframe_category software _Name NMRView _Version . loop_ _Vendor _Address _Electronic_address 'Johnson, One Moon Scientific' . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 800 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_HNCA_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCA' _Sample_label $sample_1 save_ save_3D_HNCACB_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ save_3D_CBCA(CO)NH_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 10 . mM pH 7 . pH pressure 1 . atm temperature 273 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl carbon' ppm 0 external indirect . . . 1 DSS H 1 'methyl protons' ppm 0 external indirect . . . 1 DSS N 15 nitrogen ppm 0 external indirect . . . 1 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Software_label $NMRView stop_ loop_ _Experiment_label '2D 1H-15N HSQC' '3D HNCA' '3D HNCACB' '3D CBCA(CO)NH' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name PcrG9-76 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 9 1 GLU H H 8.204 . 1 2 9 1 GLU CA C 56.987 . 1 3 9 1 GLU CB C 30.333 . 1 4 9 1 GLU N N 121.039 . 1 5 10 2 ASP H H 8.614 . 1 6 10 2 ASP CA C 54.778 . 1 7 10 2 ASP CB C 41.103 . 1 8 10 2 ASP N N 122.277 . 1 9 11 3 THR H H 8.157 . 1 10 11 3 THR CA C 62.816 . 1 11 11 3 THR CB C 69.436 . 1 12 11 3 THR N N 114.746 . 1 13 12 4 LEU H H 8.176 . 1 14 12 4 LEU CA C 56.062 . 1 15 12 4 LEU CB C 41.824 . 1 16 12 4 LEU N N 124.065 . 1 17 13 5 ARG H H 8.172 . 1 18 13 5 ARG CA C 56.871 . 1 19 13 5 ARG CB C 30.465 . 1 20 13 5 ARG N N 120.795 . 1 21 14 6 ALA H H 8.227 . 1 22 14 6 ALA CA C 53.376 . 1 23 14 6 ALA CB C 19.205 . 1 24 14 6 ALA N N 123.807 . 1 25 15 7 THR H H 8.087 . 1 26 15 7 THR CA C 63.213 . 1 27 15 7 THR CB C 69.651 . 1 28 15 7 THR N N 114.162 . 1 29 16 8 VAL H H 8.153 . 1 30 16 8 VAL CA C 63.429 . 1 31 16 8 VAL CB C 32.495 . 1 32 16 8 VAL N N 122.877 . 1 33 17 9 GLN H H 8.432 . 1 34 17 9 GLN CA C 56.622 . 1 35 17 9 GLN CB C 29.180 . 1 36 17 9 GLN N N 122.997 . 1 37 18 10 ALA H H 8.276 . 1 38 18 10 ALA CA C 53.684 . 1 39 18 10 ALA CB C 18.881 . 1 40 18 10 ALA N N 124.596 . 1 41 19 11 ALA H H 8.246 . 1 42 19 11 ALA CA C 53.655 . 1 43 19 11 ALA CB C 18.965 . 1 44 19 11 ALA N N 122.681 . 1 45 20 12 GLU H H 8.260 . 1 46 20 12 GLU CA C 57.806 . 1 47 20 12 GLU CB C 29.861 . 1 48 20 12 GLU N N 118.771 . 1 49 21 13 LEU H H 7.989 . 1 50 21 13 LEU CA C 56.111 . 1 51 21 13 LEU CB C 42.053 . 1 52 21 13 LEU N N 121.590 . 1 53 22 14 ALA H H 7.993 . 1 54 22 14 ALA CA C 53.542 . 1 55 22 14 ALA CB C 18.884 . 1 56 22 14 ALA N N 123.042 . 1 57 23 15 ILE H H 7.867 . 1 58 23 15 ILE CA C 62.136 . 1 59 23 15 ILE CB C 38.370 . 1 60 23 15 ILE N N 119.176 . 1 61 24 16 ARG H H 8.198 . 1 62 24 16 ARG CA C 57.138 . 1 63 24 16 ARG CB C 30.577 . 1 64 24 16 ARG N N 123.953 . 1 65 25 17 ASP H H 8.341 . 1 66 25 17 ASP CA C 55.251 . 1 67 25 17 ASP CB C 41.186 . 1 68 25 17 ASP N N 120.672 . 1 69 26 18 SER H H 8.233 . 1 70 26 18 SER CA C 61.253 . 1 71 26 18 SER CB C 63.409 . 1 72 26 18 SER N N 116.051 . 1 73 27 19 GLU H H 8.482 . 1 74 27 19 GLU CA C 58.174 . 1 75 27 19 GLU CB C 29.677 . 1 76 27 19 GLU N N 123.337 . 1 77 28 20 GLU H H 8.246 . 1 78 28 20 GLU CA C 58.496 . 1 79 28 20 GLU CB C 30.068 . 1 80 28 20 GLU N N 120.270 . 1 81 29 21 ARG H H 8.330 . 1 82 29 21 ARG CA C 58.563 . 1 83 29 21 ARG CB C 30.219 . 1 84 29 21 ARG N N 121.270 . 1 85 30 22 GLY H H 8.274 . 1 86 30 22 GLY CA C 46.610 . 1 87 30 22 GLY N N 106.863 . 1 88 31 23 ARG H H 7.936 . 1 89 31 23 ARG CA C 58.106 . 1 90 31 23 ARG CB C 30.409 . 1 91 31 23 ARG N N 121.936 . 1 92 32 24 LEU H H 8.107 . 1 93 32 24 LEU CA C 56.796 . 1 94 32 24 LEU CB C 41.880 . 1 95 32 24 LEU N N 121.121 . 1 96 33 25 LEU H H 8.159 . 1 97 33 25 LEU CA C 56.786 . 1 98 33 25 LEU CB C 41.779 . 1 99 33 25 LEU N N 120.940 . 1 100 34 26 ALA H H 7.941 . 1 101 34 26 ALA CA C 54.425 . 1 102 34 26 ALA CB C 18.464 . 1 103 34 26 ALA N N 121.502 . 1 104 35 27 GLU H H 8.101 . 1 105 35 27 GLU CA C 58.137 . 1 106 35 27 GLU CB C 29.893 . 1 107 35 27 GLU N N 117.924 . 1 108 36 28 MET H H 8.063 . 1 109 36 28 MET CA C 57.444 . 1 110 36 28 MET CB C 32.765 . 1 111 36 28 MET N N 120.004 . 1 112 37 29 TRP H H 8.200 . 1 113 37 29 TRP CA C 58.514 . 1 114 37 29 TRP CB C 29.261 . 1 115 37 29 TRP N N 120.375 . 1 116 38 30 GLN H H 8.089 . 1 117 38 30 GLN CA C 57.348 . 1 118 38 30 GLN CB C 28.945 . 1 119 38 30 GLN N N 120.082 . 1 120 39 31 GLY H H 7.941 . 1 121 39 31 GLY CA C 45.806 . 1 122 39 31 GLY N N 108.168 . 1 123 40 32 LEU H H 7.879 . 1 124 40 32 LEU CA C 55.336 . 1 125 40 32 LEU CB C 42.608 . 1 126 40 32 LEU N N 120.577 . 1 127 41 33 GLY H H 8.231 . 1 128 41 33 GLY CA C 45.612 . 1 129 41 33 GLY N N 108.525 . 1 130 42 34 LEU H H 7.907 . 1 131 42 34 LEU CA C 54.966 . 1 132 42 34 LEU CB C 42.542 . 1 133 42 34 LEU N N 120.971 . 1 134 43 35 ALA H H 8.220 . 1 135 43 35 ALA CA C 52.388 . 1 136 43 35 ALA CB C 19.340 . 1 137 43 35 ALA N N 124.220 . 1 138 44 36 ALA H H 8.233 . 1 139 44 36 ALA CA C 53.025 . 1 140 44 36 ALA CB C 19.234 . 1 141 44 36 ALA N N 123.030 . 1 142 45 37 ASP H H 8.193 . 1 143 45 37 ASP CA C 54.242 . 1 144 45 37 ASP CB C 41.054 . 1 145 45 37 ASP N N 118.349 . 1 146 46 38 ALA H H 8.095 . 1 147 46 38 ALA CA C 53.203 . 1 148 46 38 ALA CB C 19.217 . 1 149 46 38 ALA N N 124.087 . 1 150 47 39 GLY H H 8.331 . 1 151 47 39 GLY CA C 45.602 . 1 152 47 39 GLY N N 107.239 . 1 153 48 40 GLU H H 8.163 . 1 154 48 40 GLU CA C 56.983 . 1 155 48 40 GLU CB C 30.182 . 1 156 48 40 GLU N N 120.179 . 1 157 49 41 LEU H H 8.067 . 1 158 49 41 LEU CA C 55.504 . 1 159 49 41 LEU CB C 42.011 . 1 160 49 41 LEU N N 121.718 . 1 161 50 42 LEU H H 7.967 . 1 162 50 42 LEU CA C 55.283 . 1 163 50 42 LEU CB C 42.236 . 1 164 50 42 LEU N N 121.588 . 1 165 51 43 PHE H H 7.993 . 1 166 51 43 PHE CA C 57.808 . 1 167 51 43 PHE CB C 39.428 . 1 168 51 43 PHE N N 119.559 . 1 169 52 44 GLN H H 8.096 . 1 170 52 44 GLN CA C 55.249 . 1 171 52 44 GLN CB C 29.691 . 1 172 52 44 GLN N N 121.453 . 1 173 53 45 ALA H H 8.259 . 1 174 53 45 ALA CA C 51.119 . 1 175 53 45 ALA CB C 18.046 . 1 176 53 45 ALA N N 126.312 . 1 177 54 46 PRO CA C 63.705 . 1 178 54 46 PRO CB C 31.753 . 1 179 55 47 GLU H H 8.621 . 1 180 55 47 GLU CA C 57.799 . 1 181 55 47 GLU CB C 29.582 . 1 182 55 47 GLU N N 119.876 . 1 183 56 48 ARG H H 8.331 . 1 184 56 48 ARG CA C 57.624 . 1 185 56 48 ARG CB C 29.660 . 1 186 56 48 ARG N N 121.175 . 1 187 57 49 GLU H H 8.327 . 1 188 57 49 GLU CA C 57.821 . 1 189 57 49 GLU CB C 29.705 . 1 190 57 49 GLU N N 121.100 . 1 191 58 50 LEU H H 8.108 . 1 192 58 50 LEU CA C 56.360 . 1 193 58 50 LEU CB C 42.081 . 1 194 58 50 LEU N N 122.373 . 1 195 59 51 ALA H H 8.121 . 1 196 59 51 ALA CA C 53.591 . 1 197 59 51 ALA CB C 18.752 . 1 198 59 51 ALA N N 123.525 . 1 199 60 52 ARG H H 8.145 . 1 200 60 52 ARG CA C 57.461 . 1 201 60 52 ARG CB C 30.400 . 1 202 60 52 ARG N N 119.879 . 1 203 61 53 ALA H H 8.196 . 1 204 61 53 ALA CA C 53.981 . 1 205 61 53 ALA CB C 18.528 . 1 206 61 53 ALA N N 123.330 . 1 207 62 54 ALA H H 8.111 . 1 208 62 54 ALA CA C 54.000 . 1 209 62 54 ALA CB C 18.544 . 1 210 62 54 ALA N N 121.879 . 1 211 63 55 GLU H H 8.298 . 1 212 63 55 GLU CA C 59.086 . 1 213 63 55 GLU CB C 29.708 . 1 214 63 55 GLU N N 119.829 . 1 215 64 56 GLU H H 8.329 . 1 216 64 56 GLU CA C 59.163 . 1 217 64 56 GLU CB C 29.465 . 1 218 64 56 GLU N N 119.237 . 1 219 65 57 GLU H H 8.127 . 1 220 65 57 GLU CA C 58.928 . 1 221 65 57 GLU CB C 29.459 . 1 222 65 57 GLU N N 120.574 . 1 223 66 58 LEU H H 7.887 . 1 224 66 58 LEU CA C 57.727 . 1 225 66 58 LEU CB C 41.687 . 1 226 66 58 LEU N N 121.492 . 1 227 67 59 LEU H H 8.109 . 1 228 67 59 LEU CA C 57.686 . 1 229 67 59 LEU CB C 41.583 . 1 230 67 59 LEU N N 119.392 . 1 231 68 60 ALA H H 8.031 . 1 232 68 60 ALA CA C 55.032 . 1 233 68 60 ALA CB C 18.118 . 1 234 68 60 ALA N N 121.606 . 1 235 69 61 GLU H H 8.014 . 1 236 69 61 GLU CA C 58.746 . 1 237 69 61 GLU CB C 29.360 . 1 238 69 61 GLU N N 119.726 . 1 239 70 62 LEU H H 8.166 . 1 240 70 62 LEU CA C 58.013 . 1 241 70 62 LEU CB C 41.695 . 1 242 70 62 LEU N N 120.552 . 1 243 71 63 ARG H H 8.055 . 1 244 71 63 ARG CA C 59.043 . 1 245 71 63 ARG CB C 30.231 . 1 246 71 63 ARG N N 118.182 . 1 247 72 64 ARG H H 7.852 . 1 248 72 64 ARG CA C 58.607 . 1 249 72 64 ARG CB C 30.350 . 1 250 72 64 ARG N N 119.900 . 1 251 73 65 MET H H 8.119 . 1 252 73 65 MET CA C 57.293 . 1 253 73 65 MET CB C 32.801 . 1 254 73 65 MET N N 119.015 . 1 255 74 66 ARG H H 8.031 . 1 256 74 66 ARG CA C 56.963 . 1 257 74 66 ARG CB C 30.485 . 1 258 74 66 ARG N N 119.615 . 1 259 75 67 SER H H 8.100 . 1 260 75 67 SER CA C 59.023 . 1 261 75 67 SER CB C 63.755 . 1 262 75 67 SER N N 115.597 . 1 263 76 68 SER H H 8.261 . 1 264 76 68 SER CA C 59.014 . 1 265 76 68 SER CB C 63.835 . 1 266 76 68 SER N N 117.354 . 1 267 77 69 GLY H H 8.418 . 1 268 77 69 GLY CA C 45.535 . 1 269 77 69 GLY N N 110.969 . 1 270 78 70 SER CA C 58.626 . 1 271 78 70 SER CB C 63.797 . 1 272 78 70 SER N N 115.568 . 1 273 79 71 GLU H H 8.614 . 1 274 79 71 GLU CA C 57.095 . 1 275 79 71 GLU CB C 29.990 . 1 276 79 71 GLU N N 122.330 . 1 277 80 72 ASN H H 8.336 . 1 278 80 72 ASN CA C 53.368 . 1 279 80 72 ASN CB C 38.739 . 1 280 80 72 ASN N N 118.813 . 1 281 81 73 LEU H H 7.995 . 1 282 81 73 LEU CA C 55.256 . 1 283 81 73 LEU N N 121.962 . 1 284 82 74 TYR H H 7.954 . 1 285 82 74 TYR CA C 57.891 . 1 286 82 74 TYR CB C 38.610 . 1 287 82 74 TYR N N 119.481 . 1 288 83 75 PHE H H 7.983 . 1 289 83 75 PHE CA C 57.640 . 1 290 83 75 PHE CB C 39.606 . 1 291 83 75 PHE N N 121.314 . 1 292 84 76 GLN H H 7.740 . 1 293 84 76 GLN CA C 57.489 . 1 294 84 76 GLN N N 126.087 . 1 stop_ save_